KR102123806B1 - Composition for improvement of skin condition containing evening primrose and hydrangea flower as active ingredient and method of preparing the same - Google Patents

Abstract

The present invention relates to a composition for improving skin condition and containing a method for preparing the composition, which contains the extract of the evening primrose and hydrangea flowers as an active ingredient.
One embodiment of the present invention provides a composition for improving the skin condition containing the evening primrose and hydrangea flower extract as an active ingredient.

Description

Composition for improving skin condition containing baby primrose and hydrangea extract as an active ingredient and its manufacturing method {COMPOSITION FOR IMPROVEMENT OF SKIN CONDITION CONTAINING EVENING PRIMROSE AND HYDRANGEA FLOWER AS ACTIVE INGREDIENT AND METHOD OF PREPARING THE SAME}

The present invention relates to a composition for improving skin condition and containing a method for preparing the composition, which contains the extract of the evening primrose and hydrangea.

Recently, due to the active participation of women in society and the development of the cosmetics industry, not only women but also men became interested in young and beautiful skin, and the symbol of beautiful skin is recognized as clear and clean skin. However, when exposed to a large amount of ultraviolet light, antioxidants are reduced, reactive oxygen species (ROS) are generated in the skin, and free radicals such as hydroxyl radicals, superoxide radicals and lipids Lipid peroxide radicals are highly reactive and react with lipids, proteins, polysaccharides, and nucleic acids, which are major components of the skin, causing damage to cells and causing skin aging. In addition, the balance of the enzymatic and non-enzymatic antioxidant defenses in the skin is brought down, resulting in an oxidative stress state, resulting in rough and shiny skin, and repetition of this process causes wrinkles. On the other hand, melanin causes pigmentation on the skin and plays an important role in skin carcinogenesis. Abnormal melanin pigmentation causes skin physiological disorders related to blemishes, freckles, and age spots. Melanin synthesis in skin exposed by UV irradiation is initiated through a tyrosinase enzyme. Therefore, tyrosinase inhibitors are used for the treatment of melanin pigmentation and for skin whitening and depigmentation. However, many tyrosinase inhibitors, including arbutin, kojic acid, and linoleic acid, are widely used for skin whitening, but have many side effects.

Therefore, in order to prevent skin aging, it is necessary to suppress the generation of excess free radicals and to effectively remove the free radicals generated. Thus, the present inventors have been working on the above problem to improve the skin condition by checking the optimal mixing ratio of the evening primrose and hydrangea flower extracts to help inhibit the enzymes that cause wrinkles on the skin and inhibit the activity of the enzyme that proceeds with pigmentation. By preparing the solvent composition, the present invention was completed by discovering that the composition exhibits improved natural antioxidant and whitening functions than a single extract.

In this regard, Korean Patent Registration No. 10-0964723 discloses a cosmetic composition containing a wildflower extract.

The present invention has been devised to solve the above-mentioned problems, and an embodiment of the present invention provides a composition for improving the skin condition, which contains an extract of an evening primrose and a hydrangea flower as an active ingredient.

In addition, another embodiment of the present invention provides a method for preparing the composition for improving the skin condition.

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those having ordinary knowledge in the technical field to which the present invention belongs from the following description. There will be.

As a technical means for achieving the above-described technical problem, one aspect of the present invention,

Provided is a composition for improving skin condition, which contains the extracts of Oenothera laciniata Hill and Hydrangea macrophylla as active ingredients.

The primrose and hydrangea flower extract is mixed with dry pulverized flowers of primrose and hydrangea, water, C1-4 anhydrous or hydrous lower alcohol, acetone, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, 1,3 -It may be extracted with a solvent selected from the group consisting of butylene glycol and combinations thereof.

The mixing ratio of the crushed baby primrose and hydrangea flowers may be 50 parts by weight to 200 parts by weight of 100 parts by weight of the baby primrose.

The baby primrose and hydrangea flower extract may be one in which solvent is dissolved after concentration of the baby primrose and hydrangea flower extract under reduced pressure.

After concentration under reduced pressure, the concentration of the solvent-solvated extract may be 1 μg/mL to 150 μg/mL.

The skin condition improvement may be skin wrinkle improvement, skin aging improvement, or skin whitening.

The composition for improving skin condition may be characterized by inhibiting elastase and tyrosinase.

In addition, another aspect of the present invention,

Crushing the baby primrose and hydrangea flowers after hot air drying; Mixing the crushed baby primrose and hydrangea flowers; Extracting the mixed baby primrose and hydrangea by adding a solvent; And concentrating the extracted baby evening primrose and hydrangea flower extracts under reduced pressure.

The hot air drying may be performed at a temperature of 50°C to 80°C for 12 hours to 30 hours.

The mixing ratio of the baby primrose and hydrangea flowers may be 50 parts by weight to 200 parts by weight of 100 parts by weight of the baby primrose.

The extraction may be performed by applying ultrasonic waves.

According to an embodiment of the present invention, the composition for improving skin condition may have an improved wrinkle improvement effect, skin whitening effect and antioxidant effect compared to a single component by containing an extract of a mixture of baby primrose and hydrangea flowers.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a schematic diagram showing a method of manufacturing a composition for improving skin condition according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail. However, the present invention may be implemented in various different forms, and the present invention is not limited by the examples described herein, and the present invention is only defined by the claims below.

In addition, the terms used in the present invention are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. 'Inclusion' of a component in the specification of the present invention means that other components may be further included instead of excluding other components, unless otherwise specified.

The first aspect of the present application,

Provided is a composition for improving skin condition, which contains the extracts of Oenothera laciniata Hill and Hydrangea macrophylla as active ingredients.

Hereinafter, the composition for improving the skin condition according to the first aspect of the present application will be described in detail.

In one embodiment of the present application, the baby primrose is a plant belonging to the Asteraceae family, which helps lower cholesterol, relieves pain, has an effect on skin disease or inflammation, diabetes, high blood pressure, and chronic diseases, and is toxic to the human body. It is known to have little. In addition, the hydrangea is an ornamental shrub belonging to the family Saxifragaceae, and the color of the flower changes from white to neutral if it is neutral, blue to acidic, and pink to alkaline. It is known that the outpost is medicinal and is used for medicinal purposes, and it is known to have an effect of treating malignancies, cardiac calorimeters, kidney cysts, and burns. There are alkaloids that act as anti-malaria on leaves and flowers. Rutin on flowers, daphnetin and umbelliferone on roots, hydrangenol, and hydragenic acids, Lunularic acid, and leaves contain skimmin.

In one embodiment of the present application, the primrose and hydrangea flower extracts are separated using conventional solvents according to conventional methods known in the art for extracting extracts from natural products, that is, under conditions of normal temperature and pressure. can do. In addition, the extract of the baby primrose and hydrangea flowers is not limited to the extraction sites of the primrose and hydrangea flowers, but preferably means an extract obtained from the leaves, stems, roots, or flowers of the primrose and hydrangea flowers, more preferably May mean an extract obtained from an outpost consisting of leaves, stems and roots, or an outpost consisting of leaves, stems, roots and flowers. In addition, the baby evening primrose and hydrangea flowers may be preferably using Jeju-made raw materials.

In one embodiment of the present application, the primrose and hydrangea flower extracts are mixed with dry crushed products of primrose and hydrangea flowers, water, C1-4 anhydrous or hydrous lower alcohol, acetone, ethyl acetate, butyl acetate, dichloromethane , It may be extracted with a solvent selected from the group consisting of chloroform, hexane, 1,3-butylene glycol and combinations thereof. According to an embodiment of the present invention, the extraction solvent may be ethanol, wherein the concentration of the ethanol may be 50% to 90%, more preferably the concentration may be 70%.

In one embodiment of the present application, the mixing ratio of the crushed baby primrose and hydrangea flowers may be 50 parts by weight to 200 parts by weight of 100 parts by weight of primrose flowers, preferably 100 parts by weight of the primrose flowers Contrast may be 50 parts by weight of hydrangea flowers. When the mixing ratio is outside the above range, the antioxidant activity and the elastase and tyrosinase enzyme inhibitory activity of the composition for improving skin condition may be reduced.

In one embodiment of the present application, the primrose and hydrangea flower extracts may be one in which solvent is dissolved after concentration of the primrose and hydrangea flower extracts under reduced pressure.

In one embodiment of the present application, the concentration of the solvent-solvated extract after concentration under reduced pressure may be 1 μg/mL to 150 μg/mL, and preferably, the concentration may be 1 μg/mL to 100 μg/mL. have. When the concentration is less than 1 μg/mL, antioxidant activity and elastase and tyrosinase enzyme inhibitory activity of the composition for improving skin condition may be reduced.

In one embodiment of the present application, the skin condition improvement may be skin wrinkle improvement, skin aging improvement, or skin whitening.

In one embodiment of the present application, the composition for improving skin condition may be characterized by inhibiting elastase and tyrosinase. More specifically, by inhibiting the elastase, a wrinkle improvement effect may be exhibited, and by inhibiting the tyrosinase, a skin whitening effect may be exhibited.

In one embodiment of the present application, the composition for improving skin condition may be used in the form of a cosmetic composition, in which case the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, interface It can be formulated with active agent-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, and the like. More specifically, it can be prepared in the form of a flexible lotion, nutrition lotion, lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. In addition, when used as a cosmetic composition, in addition to the composition for improving skin condition and a carrier component, it may include ingredients commonly used in cosmetic compositions, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments And conventional auxiliaries such as fragrances.

In one embodiment of the present application, the composition for improving skin condition may be used as an active ingredient of a pharmaceutical composition for the treatment or prevention of melanin hyperpigmentation disease, in which case the pharmaceutical composition is for improving the skin condition In addition to the composition may include a pharmaceutically acceptable carrier. The carrier is commonly used in the formulation, for example, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, poly Vinyl pyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, and those containing a material selected from the group consisting of combinations thereof Can be. Further, the pharmaceutical composition may further include a lubricant, a wetting agent, a sweetener, a flavoring agent, an emulsifying agent, a suspending agent and a preservative in addition to the above substances.

The second aspect of the present application,

Crushing the baby primrose and hydrangea flowers after hot air drying; Mixing the crushed baby primrose and hydrangea flowers; Extracting the mixed baby primrose and hydrangea by adding a solvent; And concentrating the extracted baby evening primrose and hydrangea flower extracts under reduced pressure.

The detailed description of parts overlapping with the first aspect of the present application is omitted, but the description of the first aspect of the present application may be equally applied even if the description is omitted in the second aspect.

Hereinafter, a method for preparing a composition for improving skin condition according to the second aspect of the present application will be described in detail with reference to FIG. 1.

First, in one embodiment of the present application, the method for preparing the composition for improving the skin condition includes a step (S100) of drying the primrose and hydrangea flower after hot air drying and crushing.

In one embodiment of the present application, the hot air drying may be performed for 12 hours to 30 hours at a temperature of 50°C to 80°C, and preferably performed for 20 hours to 25 hours at a temperature of 60°C to 70°C May be In addition, the pulverization is not limited as long as it uses a conventional pulverization method.

Next, in one embodiment of the present application, the method for preparing the composition for improving skin condition includes mixing the crushed baby primrose and hydrangea flower (S200).

In one embodiment of the present application, the mixing ratio of the baby primrose and hydrangea flower may be 50 parts by weight to 200 parts by weight of 100 parts by weight of the baby primrose. When the mixing ratio is outside the above range, the antioxidant activity and the elastase and tyrosinase enzyme inhibitory activity of the composition for improving skin condition may be reduced.

Next, in one embodiment of the present application, the method for preparing the composition for improving skin condition includes a step (S300) of extracting by adding a solvent to the mixed primrose and hydrangea flowers.

In one embodiment of the present application, the extraction may be performed by applying ultrasonic waves, and the intensity of the applied ultrasonic waves may be 10 kHz to 100 kHz.

In one embodiment of the present application, the solvent is water, C1-4 anhydrous or hydrous lower alcohol, acetone, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, 1,3-butylene glycol and combinations thereof It may be using a solvent selected from the group consisting of. In addition, the content of the solvent may be carried out by adding 8 to 12 times the volume of the solvent compared to 100 g of the mixed evening primrose and hydrangea flowers, preferably by adding 10 times the volume of the solvent. .

In one embodiment of the present application, the extraction may be extracted for 30 minutes to 150 minutes at a temperature of 30°C to 50°C, and preferably for 1 hour at a temperature of 40°C. Further, the extraction may be performed repeatedly 3 to 5 times under the same conditions as above.

Next, in one embodiment of the present application, the method for preparing the composition for improving skin condition includes the step of concentrating the extracted baby primrose and hydrangea flower under reduced pressure (S400).

In one embodiment of the present application, the reduced pressure concentration may be performed according to a conventionally used method, for example, it may be performed using a reduced pressure concentration machine. In addition, the reduced pressure concentration may be performed for 6 hours to 8 hours at 40°C to 45°C using a reduced pressure concentrator after removing impurities by passing the primrose and hydrangea flower extract through a filter paper.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art to which the present invention pertains can easily practice. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein.

Example 1. Preparation of composition for improving skin condition

Baby primrose and hydrangea flowers were dried after hot air drying at 65° C. for 24 hours. After mixing the dried crushed baby primrose and hydrangea in a weight ratio of 1:1, 10 times (w/v) of 70% ethanol was added to 100 g of the mixture, and an ultrasonic disperser at 40°C (sonics vc750, USA) After extraction with ultrasonic waves at 40 kHz for 1 hour, the extraction was repeated 3 times at 1 hour intervals. The extract obtained above was concentrated using a vacuum concentrator, and then diluted by concentration in a solvent to be used as a sample.

Example 2. Preparation of composition for improving skin condition

It was prepared in the same manner as in Example 1, except that the baby primrose and hydrangea flowers were mixed in a weight ratio of 2:1.

Example 3. Preparation of composition for improving skin condition

It was prepared in the same manner as in Example 1, except that the baby primrose and hydrangea were mixed in a weight ratio of 1:2.

Comparative Example 1. Preparation of composition for improving skin condition (not including hydrangea flower extract)

It was prepared in the same manner as in Example 1 using only the baby primrose.

Comparative Example 2. Preparation of composition for improving skin condition (without evening primrose extract)

It was prepared in the same manner as in Example 1 using only hydrangea flowers.

Experimental Example 1. Evaluation of antioxidant activity

(1) DPPH Radical-Scavenging Activity assay

As the DPPH measurement method, a modified method such as Blois was used. DPPH (1,1-diphenyl-2-picrylhydrazyl) As a method of measuring the electron donating ability (EDA) for free radicals, when DPPH encounters an antioxidant activity, electrons or hydrogen are provided. The antioxidant ability of the sample was evaluated by measuring the degree of decolorization of the initial purple to yellow as the radicals were erased, that is, the degree of radical scavenging with a microplate reader.

Specifically, 180 μL of a 0.2 mM DPPH (1-diphenyl-2-picrylhydrazyl) solution for each concentration was added to 20 μL of Examples 1 to 3 and Comparative Examples 1 and 2, followed by a cancer reaction for 10 minutes, followed by a microplate reader (Model: Tecan AT/sunrise TW) and the remaining DPPH free radicals were measured at 515 nm. The absorbance value of the control was in the range of 0.94 to 0.97, and the DPPH radical scavenging activity was expressed as a percentage of the difference in absorbance between the addition and non-addition of the sample solution. As a control, ascorbic acid (Vitamin C) was used.

<Equation 1>

The results are shown in Table 1 below.

[Table 1]

As shown in Table 1, as a result of confirming the radical scavenging rate according to the sample concentration of each of the Examples and Comparative Examples, in the case of the mixed extracts (Examples 1 to 3), each individual extract (Comparative Examples 1 and 2) Higher antioxidant activity was observed, and Example 2 (baby primrose extract and hydrangea flower extract, 2:1) exhibited the highest radical scavenging rate of 52.71% at 100 μg/mL.

(2) ABTS Radical Scavenging Activity assay

ABTS+ radical scavenging activity was measured using a method such as Re. ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt] 7.0 mM was reacted with 2.45 mM potassium persulfate for 16 hours at 25°C for 16 hours to prepare ABTS+ before use When the ABTS free radical meets the antioxidant activity material, the radical is erased and decolorized from dark blue to transparent, and the degree of decolorization, that is, the degree of radical scavenging, is measured by a microplate reader. The antioxidant capacity was evaluated.

Specifically, 180 μL of radical-formed ABTS solution was added to 20 μL of Examples 1 to 3 and Comparative Examples 1 and 2 by dark reaction at room temperature for 15 minutes, and absorbance was measured at 700 nm. The absorbance of the control (control) was diluted so that the absorbance at 700 nm was 0.70±0.02. As a control, ascorbic acid (Vitamin C) was used.

<Equation 2>

The results are shown in Table 2 below.

[Table 2]

As shown in Table 2, experiments on antioxidant effects were performed using the ABTS Radical Scavenging Activity assay for each sample concentration in Examples and Comparative Examples. As a result of confirming the radical scavenging rate according to each sample concentration, in the case of the mixed extracts (Examples 1 to 3), antioxidant activity was higher than that of each of the individual extracts (Comparative Examples 1 and 2), and Example 2 ( Baby primrose extract and hydrangea extract, 2:1) showed the highest radical scavenging rate of 74.07% at 100 μg/mL.

Experimental Example 2. Evaluation of enzyme inhibitory activity

(1) Elastase inhibition assay

Elastase (Elastase) inhibitory ability was measured by applying the method of James et al. The substrate [N-succinyl-(L-Ala)3-p-nitroanilide] was dissolved in DMSO to prepare a 6.25 mM solution, and then mixed with a 0.15 M Tris-HCl buffer (buffer, pH 8.0) at a ratio of 1:12. Thereafter, 20 μL of samples according to the concentrations of Examples 1 to 3 and Comparative Examples 1 and 2 and 130 μL of a substrate in which a buffer was mixed were added to a 96 well plate, and absorbance was measured at 405 nm. After (Blank), 50 μL of Elastase (Porcine pancreas elastase, PPE) mixed with a buffer was added and reacted at 25° C. for 15 minutes, followed by absorbance at 405 nm through a microplate reader. Was measured. The degree of inhibitory activity is expressed in percent (%), and the percentage value was calculated in the same manner as in Equation 3 below. As a control of Elastase, oleanolic acid was used.

<Equation 3>

In Equation 3, S is the sample absorbance, SB is the absorbance of the sample before adding the enzyme, C is the absorbance of the control, and CB is the absorbance of the control before adding the enzyme.

The results are shown in Table 3 below.

[Table 3]

As shown in Table 3, the experiments for wrinkle-related enzyme inhibition activity were performed using Elastase inhibition assay for each sample concentration in Examples and Comparative Examples. As a result of confirming the enzyme inhibitory activity according to the concentration of each sample, in the case of the mixed extract (Examples 1 to 3), the enzyme inhibitory activity was higher than that of each of the individual extracts (Comparative Examples 1 and 2), and Example 2 (Baby Evening Primrose) Extract and Hydrangea Flower Extract, 2:1) showed the highest Elastase inhibitory activity of 66.24% at 100 μg/mL.

(2) Tyrosinase inhibition assay

The measurement of tyrosinase inhibitory activity was used by applying the method of Ishihara et al. The enzyme solution was prepared at 250 unit/mL by adding 0.1 M potassium phosphate buffer solution (pH 6.8) to the tyrosinase (Mushroom tyrosinase) enzyme. Then, 20 μL of samples according to concentrations of Examples 1 to 3 and Comparative Examples 1 and 2 were mixed in a 96 well plate, and 130 μL of 2 mM L-tyrosin was mixed and measured at 480 nm (Blank). ), 50 μL of 250 unit/mL tyrosinase enzyme solution was added, reacted at 35° C. for 10 to 15 minutes, and measured at 480 nm (Data). The absorbance of the reference value (control) was in the range of 0.3 to 0.4. The degree of inhibitory activity is expressed in percent (%), and the percentage value was calculated in the same manner as in Equation 4 below. Arbutin was used as a control for tyrosinase.

<Equation 4>

In Equation 4, S is the sample (sample) absorbance, SB is the sample absorbance before the enzyme (sample), C is the absorbance of the reference value (control), and CB is the absorbance of the reference value (control) before adding the enzyme.

The results are shown in Table 4 below.

[Table 4]

As shown in Table 4, an enzyme inhibition activity experiment related to melanin production was performed using a Tyrosinase inhibition assay for each sample concentration in Examples and Comparative Examples. As a result of confirming the enzyme inhibitory activity according to the concentration of each sample, in the case of the mixed extract (Examples 1 to 3), the enzyme inhibitory activity was higher than that of each single extract (Comparative Examples 1 and 2), and Example 2 (Baby Evening Primrose) Extract and Hydrangea Flower Extract, 2:1) showed the highest tyrosinase inhibitory activity of 23.79% at 100 μg/mL.

The above description of the present invention is for illustration only, and those skilled in the art to which the present invention pertains can understand that the present invention can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all modifications or variations derived from the meaning and scope of the claims and their equivalent concepts should be interpreted to be included in the scope of the present invention.

Claims (11)

As a cosmetic composition for improving skin wrinkles and whitening skin, which contains extracts of Oenothera laciniata Hill and Hydrangea macrophylla as active ingredients,
The primrose and hydrangea flower extract is mixed with dry pulverized flowers of primrose and hydrangea, water, C1-4 anhydrous or hydrous lower alcohol, acetone, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, 1,3 -It is extracted with a solvent selected from the group consisting of butylene glycol and combinations thereof,
The mixing ratio of the crushed baby primrose and hydrangea flowers is 50 parts by weight to 200 parts by weight of 100 parts by weight of the baby primrose,
The cosmetic composition for skin wrinkle improvement and skin whitening is characterized in that it inhibits elastase and tyrosinase.
delete delete According to claim 1,
The baby primrose and hydrangea flower extract is a cosmetic composition for improving skin wrinkles and skin whitening, which is a solvent-soluble solution after concentration of the baby primrose and hydrangea flower extract under reduced pressure.
The method of claim 4,
After concentration under reduced pressure, the concentration of the solvent-solvated extract is 1 μg/mL to 150 μg/mL, which is a cosmetic composition for improving skin wrinkles and whitening the skin.
delete delete Crushing the baby primrose and hydrangea flowers after hot air drying;
Mixing the crushed baby primrose and hydrangea flowers;
Extracting the mixed baby primrose and hydrangea by adding a solvent; And
Concentrating the extracted baby primrose and hydrangea flower extracts under reduced pressure;
As a method for producing a cosmetic composition for improving skin wrinkles and whitening skin, comprising:
The solvent is a material selected from the group consisting of water, C1-4 anhydrous or hydrous lower alcohol, acetone, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, 1,3-butylene glycol and combinations thereof. To include,
The mixing ratio of the baby primrose and hydrangea flowers is 50 parts by weight to 200 parts by weight of 100 parts by weight of the baby primrose,
The cosmetic composition for skin wrinkle improvement and skin whitening is characterized by inhibiting elastase and tyrosinase.
The method of claim 8,
The hot air drying is performed for 12 hours to 30 hours at a temperature of 50 ℃ to 80 ℃ method for producing a cosmetic composition for skin wrinkle improvement and skin whitening.
delete The method of claim 8,
The extraction is a method of manufacturing a cosmetic composition for improving skin wrinkles and whitening skin, which is performed by applying ultrasound.

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