WO2011129428A1

WO2011129428A1 - Cosmetic composition containing plum extract

Info

Publication number: WO2011129428A1
Application number: PCT/JP2011/059358
Authority: WO
Inventors: 浩一瀬田; 暢浩加藤; 大二郎大谷; 貴也峯尾; 郁子安達; 文佐々木
Original assignee: ワミレスコスメティックス株式会社
Priority date: 2010-04-15
Filing date: 2011-04-15
Publication date: 2011-10-20
Also published as: JP4795475B1; JP2013163643A

Abstract

Disclosed is a composition for external application to the skin, which contains an effective amount of an extract of at least one part selected from a root, a branch and a tree bark of plum (Prunus mume) with an aqueous solvent. The composition is particularly useful for the inhibition of an elastase activity and/or the inhibition of a hyaluronidase activity. The plum extract can be used for anti-aging purposes and/or skin whitening purposes.

Description

Cosmetic composition containing ume extract

The present invention relates to a composition containing extracts from various parts of ume. The composition of the present invention is particularly useful as a cosmetic or a food or drink.

While many umes are cultivated to harvest edible ume fruits, umes are discarded as edible seeds, branches, leaves, trunks, roots, etc. resulting from pruning or replanting due to economic life. There are also many sites.

Regarding effective use of ume seeds, for example, Patent Document 1 provides a cosmetic characterized by blending a ume seed extract. Here, radical scavenging action, collagenase activity inhibitory action (collageno kit CLN-100) was obtained from an extract obtained from ground pulverized seeds of ume (variety unknown) using water, ethanol, 1,3-butylene glycol, or methanol as a solvent. Use: It seems to be using fluorescently labeled type I collagen.), Hyaluronidase activity inhibitory action (details are unknown, such as origin of hyaluronidase used in the measurement), changes in cell hue in B16 cells confirmed Has been. In addition, lotions, shampoos, creams, body gels, hair packs, and granular bath preparations containing ume seed extract have been prepared, and sensory tests have confirmed the feeling of use.

Patent Documents 2 to 4 include at least one selected from the group consisting of plum tree trunks, plum tree branches, plum tree leaves, plum tree stems, plum tree roots, plum meat, ume seed shells and ume seeds. Antioxidant, gastric mucosa damage inhibitor, diabetic neurological disease preventive agent, blood sugar level increase inhibitor, alcohol absorption inhibitor, platelet aggregation promoter, liver inflammation inhibitor, Cosmetics are described as anti-inflammatory agents, melanin pigmentation inhibitors. In these, methanol extract of Minamitakaume Jin and / or leaf stem part has an effect on gastric mucosal damage by methanol in rats, a radical (DPPH) elimination effect, an inhibitory effect on blood sugar level increase after glucose load in healthy rats, Inhibition of aldose reductase, promotion of aggregation when platelets of healthy rabbits were washed with platelet-activating factor (PAF), suppression of D-galactosamine / LPS-induced liver inflammation in healthy mice, D-in rat primary cultured hepatocytes Inhibition of galactosamine-induced acute hepatitis and inhibition of NO acid from macrophages in the abdominal cavity of healthy mice by stimulation with LPS, tyrosinase inhibitory action, radical (DPPH) elimination action, and washing of healthy rabbits of ume flower methanol extract Aggregation-promoting action has been confirmed when platelets are stimulated with platelet-activating factor (PAF)The compounds presumed to be involved in such actions include 3β-hydroxy-12-olean-28-oic acid, 2 '' '-O-Acetylrutin, Eugenylglucoside, Benzyl Glucopyranoside, and Bezyl alcohol xylosyl ( 1 → 6) Glucside etc. are mentioned.

Further, Patent Document 5 describes a collagen production promoter characterized by containing an extract from the fruit part of ume as an active ingredient. Here, human fibroblasts are extracted from a dried fruit juice obtained by washing, crushing, and enucleating the ume (variety unknown) fruit juice and using a mixed solvent of ethanol and water. Has been confirmed to promote collagen production (using anti-human collagen type I rabbit IgG).

JP 2002-284633 A JP 2000-239297 A JP 2002-370922 A JP 2004-115542 A JP 2006-176425 A

The present inventors have focused on a material called ume having a high pharmacological effect, and have developed a cosmetic characterized by comprising a fermented ume juice (No. 2526362). In this study, we studied the bioactivity of extracts from various parts from a variety of ume varieties. Then, in addition to the high collagenase activity inhibitory action, the present inventors have completed the present invention by discovering previously unknown actions such as elastase activity inhibitory action, gelatinase activity inhibitory action, Maillard reaction inhibitory action and the like.

The present invention provides the following:
[1] A group consisting of water, water vapor, purified water, mineral water, ethanol, propylene glycol, and 1,3-butylene glycol from one or more sites selected from roots, branches and bark of ume A cosmetic composition containing, as a dry powder, 0.05 to 250 mg / 100 g of an extract from one or more solvents selected from the group consisting of 0.015 to 5 g / 100 g of an emulsifier.

[2] The composition according to [1], wherein the emulsifier is lysolecithin, PEG-60 hydrogenated castor oil, hydrogenated lecithin, soybean sterol, or any combination thereof.
[3] The composition according to [1], wherein a water extract and an ethanol extract of ume root are blended.
[4] applying an ethanol extract and / or an aqueous extract of ume root to the subject's skin, thereby inhibiting elastase activity and / or hyaluronidase activity in the subject's skin and A method of treating a skin condition that is improved by inhibiting hyaluronidase activity.
[5] The method according to [4], which is a method for suppressing the progress of aging.
[6] The method according to [4] or [5], further comprising a step of suppressing Maillard reaction or melanin production in the subject's skin, which is also a method for whitening the skin.

FIG. 1 is a photograph showing the results of a gelatinase activity inhibitory effect test. FIG. 2 is a photograph showing the results of the Maillard reaction inhibitory effect test. FIG. 3 is a photograph showing the results of a test for examining the amount of emulsifier. FIG. 4 is a photograph showing the results of a test for examining the amount of emulsifier.

In the present invention, “ume” refers to ume (plum, scientific name: Prunus mume), which is a deciduous tree of the genus Rosaceae. Ume can be roughly divided into flower plums and real plums.

Hanaume is further divided into 3 types (Noume, Ume and Bungo). Examples of Noume series are single winter solstice, full moon, long sleep, cold clothes, double winter solstice, Hanakami, moon palace, Mitaka Tamagaki, as you wish, Yae Kaido, red brush, Yae red brush, Jade fist, white Namba , Tsuki no Katsura, Tsukikage, green, examples of Ume series (Red plum series) are Red Chizuru, Tamamitsu, Karaume, Kagoshima, Tsuji no Tsuji, Kuroun, and examples of Bungo series are Manazuru, Yoneha They are butterflies, sea cucumber plums, wisteria peony, white lions, Yae fried feathers, kimonos, Musashino, Ichinotani, Gangnam Mujo, and Mikuni. The Haname-Bungo system can be further divided into Bungo and Apricot.

Examples of real plums are Bungo, Shirakaga, Bakujuku, Moon World, Koshu Minato, Tammei, Kojo, and Minami High School.

In the present invention, the term “Bungo” for ume varieties refers to Bungo classified as real plum, unless otherwise specified.

In the present invention, “ume” refers to a plant body of a ume plant or a part thereof unless otherwise specified. “Parts” include fruits, seeds, organs or parts thereof (including leaves, roots, stems, flowers, stamens, pistils, fragments thereof), plant culture cells, callus, protoplasts, transformed plant cells, traits Converted plants are included.

Referring to ume in the present invention, “Wakae” refers to a branch in which a new tree that grows in early spring begins to grow and grows about 5 to 30 cm, and the skin is soft, unless otherwise specified. With respect to plums in the present invention, the term “root” includes fine roots, medium roots and thick roots, as well as old roots and others. Fine roots are those with a diameter of less than 3 mm. The middle root means one having a diameter of 3 mm or more and less than 10 mm. A radish is one having a diameter of 10 mm or more. Old root is a general term for roots obtained from ume plants over 30 years old.

In the present invention, any part of ume can be used effectively, but from the viewpoint that various effects can be expected, roots and branches (especially young branches) are preferable, and roots are more preferable.

In the present invention, “elastase” refers to a protease having elastin as a typical substrate, unless otherwise specified. Elastin is a kind of a hard protein that is rich in elasticity and constitutes connective tissues, tendons, aortic skin, cervical cord and the like of higher animals. There are many crosslinks between peptide chains constituting this protein, which brings about elasticity.

The presence or absence of the ability to inhibit the target elastase activity and / or the degree thereof can be appropriately confirmed and measured by those skilled in the art using known means. For detailed conditions, reference can be made to the Examples section of this specification. In the present invention, the presence and / or degree of inhibition of elastase activity is based on the method described in the examples of the present specification, unless otherwise specified.

In the present invention, the term “gelatinase” is a protease using a type IV collagen that is a basement membrane component as a typical substrate, unless otherwise specified, and is a matrix metalloproteinase (MMP) group of MMP-2 and MMP-9. Is included.

The presence or absence and / or degree of the ability to inhibit the target gelatinase activity can be confirmed and measured appropriately by those skilled in the art using known means. For example, it can be carried out using a commercially available MMP marker for gelatin zymography, which contains MMP-2, ProMMP-2, ProMMP-9 or a mixture thereof. For detailed conditions, reference can be made to the Examples section of this specification. In the present invention, the presence and / or degree of inhibition of gelatinase activity is based on the method described in the examples of the present specification, unless otherwise specified.

In the present invention, “collagenase” refers to a type of protease using type I collagen as a typical substrate, unless otherwise specified. Collagenase includes MMP-1, which is a matrix metalloproteinase (MMP) that degrades type I and type III collagen, which are the main components of the skin matrix. Collagen is a main component of the extracellular matrix of animals, and is known to have a physical function of maintaining the structure and exhibit cell adhesion activity. It is also contained in large amounts in the skin and is related to elasticity and strength. There are I to VIII in collagen.

The presence or absence and / or degree of the ability to inhibit the target collagenase activity can be confirmed and measured appropriately by those skilled in the art using known means. For detailed conditions, reference can be made to the Examples section of this specification. In the present invention, the presence or absence and / or degree of inhibition of collagenase activity is based on the method described in the examples of the present specification, unless otherwise specified.

In the present invention, the term “hyaluronidase” refers to an enzyme that lowers the molecular weight of hyaluronic acid, unless otherwise specified, and an enzyme that hydrolyzes the N-acetyl-D-hexosaminide bond of hyaluronic acid. Hyaluronidase is present in the skin. Hyaluronic acid is a kind of glycosaminoglycan having a repeating structure of only disaccharides of O-β-D-glucuronosyl (1 → 3) -N-acetyl-β-D-glucosaminyl (1 → 4) units. It is present in connective tissues such as animal joint fluid and dermis.

The presence or absence and / or degree of the ability to inhibit the target hyaluronidase activity can be appropriately confirmed and measured by those skilled in the art using known means. For detailed conditions, reference can be made to the Examples section of this specification. In the present invention, the presence and / or degree of inhibition of hyaluronidase activity is based on the method described in the examples of the present specification, unless otherwise specified.

In the present invention, “fibroblast activation (activity, effect)” means that the proliferation ability of normal human dermal fibroblasts (NHDF) can be enhanced. The presence / absence / degree of cell proliferation can be determined by counting the number of living cells. Various methods are known for determining the number of viable cells.

The presence and / or degree of the ability to activate the target fibroblasts can be confirmed and measured appropriately by those skilled in the art using known means. For detailed conditions, reference can be made to the Examples section of this specification. In the present invention, the presence or absence and / or degree of fibroblast activation activity is based on the method described in the examples of the present specification, unless otherwise specified.

In the present invention, “melanin production inhibition” refers to inhibition of melanocyte activator synthesis, inhibition of melanocyte activator, tyrosinase synthesis / maturation inhibition, melanin synthase (tyrosinase etc.) activity inhibition, melanin, unless otherwise specified By one or a plurality of actions selected from the group consisting of inhibition of granule uptake and promotion of degradation of melanin granules, it means that formation of melanin granules is finally suppressed or melanin granules are reduced. Melanin is an oxidation polymer derived from tyrosine. In vertebrates, melanogenesis occurs in melanophores and melanocytes differentiated from neural crest-derived melanoblasts. Within the cell, the aromatic amino acid tyrosine changes stepwise within the melanosome, and finally polymerizes non-enzymatically into melanin, followed by the formation of melanin granules.

On the other hand, substances that are toxic to melanin-producing cells may be able to suppress melanin production, but are not preferred as an external preparation for skin. Therefore, it is preferable that the component of the agent of the present invention not only has high melanin production inhibitory activity but is not cytotoxic. Such a preferable effect can be represented by a value obtained by multiplying the melanin production inhibition rate and the cell survival rate, as described in Examples described later.

A person skilled in the art confirms and measures the presence and / or degree of the ability to suppress melanin production and the presence and / or degree of cytotoxicity using known means as appropriate, for example, using B16 melanoma cells. can do. For detailed conditions, reference can be made to the Examples section of this specification. In the present invention, the presence and / or degree of melanin production inhibitory activity is based on the method described in the examples of the present specification, unless otherwise specified.

In the present invention, “Maillard reaction” refers to a reaction in which a brown substance (melanoidin) is generated from a sugar and a protein by an aminocarbonyl reaction, unless otherwise specified. The Maillard reaction is usually a non-enzymatic reaction in which an Amadori rearrangement product (Amadori compound) is generated after a reducing sugar reacts with an amino group of a protein to form a Schiff base. Then, through complex reactions such as oxidation, dehydration, condensation, intramolecular and intermolecular cross-linking, the mixture gradually becomes yellowish brown and AGEs (advanced glycation end products) which are late stage products of the Maillard reaction are produced.

The Maillard reaction that occurs in vivo is considered to be involved in the formation of brown spots on the skin seen in diabetic patients. AGE is included in the pigment that causes brown spots. In vivo Maillard reaction has also been shown to be associated with lifestyle-related diseases, diseases accelerated by aging (eg, diabetic complications, Alzheimer's disease, arteriosclerosis). AGE is a generic name for various structures produced from the Amadori rearrangement product through complex reactions such as oxidation, dehydration, and condensation, including carboxymethyllysine, pyralin, pentosidine, croslin, imidazolone, etc. A polymer of protein dimer or higher is also known as AGE.

The presence and / or extent of the subject's ability to suppress the Maillard reaction is confirmed and measured by a person skilled in the art as appropriate using known means, for example, inhibition of Amadori rearrangement product production or inhibition of AGE production as an indicator. be able to. For detailed conditions, reference can be made to the Examples section of this specification. In the present invention, when the presence and / or degree of Maillard reaction suppression ability is mentioned, it is based on the method described in the examples of the present specification, unless otherwise specified.

In the present invention, the term “antioxidation” means that the active oxygen is suppressed (not generated, erased, or suppressed) unless otherwise specified.

A person skilled in the art can appropriately confirm and measure the presence and / or degree of the antioxidant ability of a subject using known means. For example, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, superoxide dismutase (SOD) -like action, hydrogen peroxide (H ₂ O ₂ ) scavenging, TRAP (Total Radical-trapping Antioxidant Parameter), FRAP (Ferric Reducing Ability of Plasma), β-carotene fading, etc. can be used as indicators.

In the present invention, when referring to “antioxidation”, detailed conditions for scavenging DPPH radicals, SOD-like action and hydrogen peroxide (H ₂ O ₂ ) can be referred to the Examples section of this specification. it can.

In the present invention, the presence or absence and / or degree of antioxidant ability refers to the methods described in the examples of the present specification (DPPH radical elimination method, SOD method, H ₂ O ₂ elimination method) unless otherwise specified. It depends on either.

In the present invention, “suppressing the progress of aging” means delaying the progress of aging. Suppression of the progress of aging is sometimes expressed as anti-aging or anti-aging.

The extract of ume that can be used in the composition of the present invention refers to a product obtained by extracting each part of the ume with a solvent (including liquid and gaseous states). You may use each part of a ume after freeze-drying. Examples of the solvent to be extracted include water (including a liquid obtained by subjecting a specific portion of ume to steam distillation), steam, purified water, mineral water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), polyhydric alcohols (glycerin, propylene glycol, 1,3-butylene glycol, etc.), ketones (acetone, methyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, etc.) ). Preferred are polar solvents such as steam, purified water, steam distilled liquid, mineral spring water, lower alcohols, polyhydric alcohols, particularly preferably purified water, steam distilled liquid, mineral water, ethanol, propylene glycol, 1,3 -Butylene glycol is good. These solvents may be used alone or in combination of two or more. The composition of the present invention may also contain an extract obtained from each of a plurality of extraction solvents.

When the composition of the present invention contains an ethanol extract (an extract using 90% or more of ethanol), according to the study by the present inventors, the ume ethanol extract contains a hardly water-soluble component. Therefore, in order to solubilize in the composition and enhance the stability, it is preferable to further include an emulsifier as described later.

According to the study by the present inventors, an ethanol extract (an extract using ethanol having a purity of 90% or more) and water (including a liquid obtained by subjecting a specific part of ume to steam distillation) are extracted. In the particularly preferable embodiment of the composition of the present invention, it is preferable to include both an ethanol extract and a water extract.

In the present invention, when expressing the purity of the solvent and the concentration of the mixed solvent (for example, 95% ethanol), it is based on the volume unless otherwise specified.

In a particularly preferred embodiment of the present invention, a steam distilled liquid is used as an extraction solvent and is added to the composition as it is. The steam distilled liquid is obtained by continuously applying heated steam to a target (for example, ume fruit or ume shoots) in a container and cooling and collecting the steam flowing out from the container. The obtained liquid may contain various components derived from the subject.

The composition of the present invention is composed of two or more components, preferably in a form suitable for external use on the skin or as a food, and more preferably in the form of a cosmetic or health food. The composition or agent of the present invention does not include existing cosmetics and the like. Regarding the present invention, the description of the composition also applies to the agent unless otherwise specified.

In the composition of the present invention, the content of the ume extract is not particularly limited as long as the intended effect is exhibited. In addition, when mentioning the content ratio or density | concentration of the ume extract in an agent by this invention, it is the value calculated based on the weight of the ume extract with respect to the composition total weight except the case where it describes especially. At this time, unless otherwise specified, the weight of the ume extract was soaked in a solvent so that the dried ume portion was 5% W / W, and was kept at 20 to 40 ° C. for 3 to 5 days (medium 2 to 4). It is the weight expressed as the filtrate or dried product of the extract obtained by extraction between the two days. That is, the extract or dry matter obtained under other conditions is converted into the equivalent amount under the above conditions.

The composition of the present invention includes fats, waxes, hydrocarbons, fatty acids, alcohols, esters, amino acids, vitamins, which are components used in ordinary external preparations, as long as the desired effects are not impaired. , Surfactants, pH adjusters, antiseptics, fragrances, moisturizers, powders, UV absorbers, thickeners, pigments, antioxidants, whitening agents, anti-inflammatory agents, anti-wrinkle agents, rough skin improvers, Components such as acne agents, alkalis, chelating agents, sequestering agents and the like can also be blended. In addition, ingredients such as sodium sulfate, sodium hydrogen carbonate, calcium carbonate, potassium chloride, oily ingredients, emulsifiers, succinic acid, herbal medicines, inorganic pigments, fragrances, and pigments, which are components used in ordinary bath additives, You can also.

The composition of the present invention can also be prepared into various dosage forms such as a solid agent, a semi-solid agent, and a liquid agent depending on the purpose of use. The composition of the present invention can be in any form of cosmetics, quasi drugs, and pharmaceuticals. In the case of cosmetics, as skin care cosmetics, facial soap, facial cream, facial cleansing foam, lotion (replenishes the skin with moisture and moisturizing ingredients and keeps the skin fresh and is usually a transparent or translucent liquid. Depending on the intended use. , Moisturizing / softening, astringent, wiping, etc.), beauty essence (essence) (Liquid, like liquid lotion, higher in viscosity than lotion, combined with moisturizing function and emollient effect) .), Packs, massage creams, milky lotions, moisturizing creams, lip balms, etc., makeup cosmetics as foundation, white powder, lipstick, cheeks, eye shadows, etc., body care cosmetics such as soap, liquid detergent, sun cream, bathing agent, etc. , Shampoo, rinse, hair treatment, hair conditioner, hair tonic, as hair care cosmetics Hair dye, can be a scalp treatment and the like. Moreover, when it is a pharmaceutical, it can be set as a plaster, an ointment, a poultice, a liniment, and a lotion. In addition, when it mentions about the form of cosmetics in this specification, it is based on a cosmetics dictionary (Japan Cosmetics Engineers Association, 2003) unless otherwise specified.

When the composition of the present invention is in the form of a cosmetic liquid, an amount corresponding to the steam extract obtained by the method described in Example 1 of the present invention (preferably, a steam distilled liquid of ume shoots). 10 to 50 g / 100 g, preferably 15 to 45 g / 100 g, more preferably 20 to 40 g / 100 g, and a steam distilled liquid obtained by the method described in Example 1 of the present invention (preferably , A steam distilled liquid of ume fruit) can be blended in an amount of 35 to 65 g / 100 g, preferably 40 to 60 g / 100 g, more preferably 45 to 55 g / 100 g. As an amount corresponding to the dried powder of ume extract obtained by the method described above (preferably, dried powder of ume root extract with 95% ethanol), 0.05 to 250 mg / 100 g, preferably 0.1 to 15 mg / 100 g, More preferably, 1-10 mg / 100 g can be blended, and the method described in Example 1 of the present invention As an amount corresponding to the extract obtained in (preferably ume root, purified water extract), 0.05 to 15 g / 100 g, preferably 0.1 to 10 g / 100 g, more preferably 0.5 to 5 g / 100 g may be blended. it can. Also in this case, each may be blended alone in the above amounts, or may be blended in combination in the above amounts. It is more preferable to combine them. Furthermore, you may mix | blend combining the dried material of a ume extract. In addition, when referring to the blending amount of the ume extract in the present invention, it means an amount corresponding to the extract obtained by the method described in Example 1 of the present specification, unless otherwise specified.

When the composition of the present invention is in the form of a skin lotion, the ume extract is extracted with the ume extract obtained by the method described in Example 1 of the present invention (preferably, extraction of ume root with a ume shoot branch steam distillate). The amount corresponding to 0.1 to 20 g / 100 g, preferably 0.5 to 10 g / 100 g, more preferably 1 to 5 g / 100 g, was obtained by the method described in Example 1 of the present invention. The amount corresponding to dry powder of ume extract (preferably, dry powder of extract of ume root with 95% ethanol) is 0.1 to 250 mg / 100 g, preferably 0.3 to 15 mg / 100 g, more preferably 0.5 to 10 mg. / 100g can be blended. The ume extract and the dried product may be blended alone in the above amounts or in combination. It is more preferable to combine them.

In the case of another embodiment of the skin lotion, the ume extract corresponds to the ume extract obtained by the method described in Example 1 of the present invention (preferably, the extract of the ume root by the ume shoot water vapor distillation solution). 0.01 to 30 g / 100 g, preferably 0.05 to 20 g / 100 g, more preferably 0.1 to 10 g / 100 g, and the amount of ume extract obtained by the method described in Example 1 of the present invention. 0.10-250mg / 100g, preferably 0.20-70mg / 100g, more preferably 0.35-35mg / 100g as an amount corresponding to dry powder (preferably, dried powder of ume root extract with 95% ethanol) In addition, the amount corresponding to the ume steam distillate (preferably ume fruit steam distillate) obtained by the method described in Example 1 of the present invention is 5 to 70 g / 100 g, preferably 10 to 60 g / 100 g, more preferably 20 to 50 g / 100 g can be blended. Also in this case, each extract may be blended alone in the above amount, or may be blended in combination in the above amount. It is more preferable to combine them.

When the composition of the present invention is in the form of an emulsion, the ume extract is used as an amount corresponding to the ume steam distillate (preferably the ume fruit steam distillate) obtained by the method described in Example 1 of the present invention. 20 to 80 g / 100 g, preferably 30 to 70 g / 100 g, more preferably 40 to 60 g / 100 g.

In the case of the emulsion of another aspect, the ume extract corresponds to the ume extract obtained by the method described in Example 1 of the present invention (preferably, the extract of the ume root by the ume shoots steamed distillate). 0.1 to 20 g / 100 g, preferably 0.5 to 10 g / 100 g, more preferably 1 to 5 g / 100 g, and the dried ume extract obtained by the method described in Example 1 of the present invention. As an amount corresponding to powder (preferably dry powder of extract of ume root with 95% ethanol), 0.01 to 250 mg / 100 g, preferably 0.05 to 10 mg / 100 g, more preferably 0.2 to 5 mg / 100 g Can do. Also in this case, each extract may be blended alone in the above amount, or may be blended in combination in the above amount. It is more preferable to combine them.

When the composition of the present invention is in the form of a multilayer cream, it corresponds to a ume extract obtained by the method described in Example 1 of the present invention (preferably, an extract of ume roots using a ume sap branch steam distillation solution). The amount can be 0.05 to 10 g / 100 g, preferably 0.1 to 5 g / 100 g, more preferably 0.5 to 2 g / 100 g, and the dried ume extract obtained by the method described in Example 1 of the present invention. As an amount corresponding to powder (preferably, dried powder of ume root extract with 95% ethanol), 0.01 to 250 mg / 100 g, preferably 0.05 to 5 mg / 100 g, more preferably 0.3 to 1 mg / 100 g Can do. Also in this case, each extract may be blended alone in the above amount, or may be blended in combination in the above amount. It is more preferable to combine them.

When the composition of the present invention is in the form of a lip base, dried powder of ume extract obtained by the method described in Example 1 of the present invention (preferably dried powder of ume root extract with 95% ethanol) ) In an amount of 0.05 to 250 mg / 100 g, preferably 0.1 to 10 mg / 100 g, more preferably 0.5 to 5 mg / 100 g, and the ume obtained by the method described in Example 1 of the present invention. An extract (preferably, an extract of ume roots with a ume shoot steamed distillate and / or a purified water extract of ume roots) can be blended. Also in this case, each extract may be blended alone in the above amount, or may be blended in combination in the above amount. It is more preferable to combine them.

When the composition of the present invention is a soap, the ume extract is converted into a ume extract obtained by the method described in Example 1 of the present invention (preferably, an extract of ume roots using a ume sap branch steam distillation solution). As a corresponding amount, 0.01 to 30 g / 100 g, preferably 0.5 to 20 g / 100 g, more preferably 1 to 10 g / 100 g can be blended, and the ume extract obtained by the method described in Example 1 of the present invention. As an amount corresponding to the dry powder (preferably, dried powder of ume root extract with 95% ethanol) 0.01 to 250 mg / 100 g, preferably 0.05 to 10 mg / 100 g, more preferably 0.1 to 5 mg / 100 g can do. Also in this case, each extract may be blended alone in the above amount, or may be blended in combination in the above amount. It is more preferable to combine them.

When the composition of the present invention is in the form of a cosmetic, in a particularly preferred embodiment, in addition to the ume extract as an active ingredient, an effective amount of an emulsifier is included to solubilize the ume extract. The coexistence of emulsifiers is various effects of ume extract, namely elastase activity inhibition effect, gelatinase activity inhibition effect, collagenase activity inhibition effect, hyaluronidase activity inhibition effect, fibroblast activation (activity, effect), melanin production inhibition effect, Maillard The reaction suppressing effect and the antioxidant effect can be synergistically enhanced.

Examples of emulsifiers that can be used in the present invention are lecithin, lecithin derivatives, lysophospholipid, polymer emulsifier, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, sorbitan fatty acid ester, poly Oxyethylene sorbitan fatty acid ester, polyoxyethylene sorbit fatty acid ester, polyoxyethylene lanolin, lanolin alcohol, beeswax derivative, polyoxyethylene hydrogenated castor oil, polyoxyethylene sterol, hydrogenated sterol, polyoxyethylene alkyl ether, polyoxyethylene poly Oxypropylene alkyl ether, polyoxyethylene alkyl ether phosphate / phosphate, polyethylene glycol fatty acid ester It is an ether. Particularly preferred examples are lecithin and lecithin derivatives. Lecithin derivatives include fractionated lecithin, enzymatically degraded lecithin (including lysolecithin), and hydrogenated lecithin (sometimes referred to as “hydrogenated lecithin”). More specific preferable examples of the emulsifier that can be used in the present invention are expressed as lysolecithin, hydrogenated lecithin, hydrogenated lysolecithin (lysophospholipid, hydrogenated phospholipid, hydrogenated lysophospholipid) derived from soybean or egg yolk. There is also.). Lecithin and lecithin derivatives can also be used in combination.

Particularly preferred examples of emulsifiers include lysolecithin, PEG-60 hydrogenated castor oil, hydrogenated lecithin, soybean sterol, or any combination thereof in an amount effective to solubilize the ume extract in the composition of the present invention. When an emulsifier is included, the blending amount is 0.015 g / 100 g or more, preferably 0.025 g / 100 g, more preferably 0.05 g / 100 g. In any case, it is preferably 5 g / 100 g or less, more preferably 2.5 g / 100 g or less. The upper limit value may be determined in consideration of viscosity.

When the emulsifier is a lecithin derivative, the composition of the present invention may contain 0.001 to 10 g / 100 g, preferably 0.002 to 5 g / 100 g, more preferably 0.002 to 3 g / 100 g.

When the composition of the present invention is in the form of a cosmetic, in a particularly preferred embodiment, it is used immediately after washing the face. According to such usage, various effects of ume extract, namely elastase activity inhibitory effect, gelatinase activity inhibitory effect, collagenase activity inhibitory effect, hyaluronidase activity inhibitory effect, fibroblast activation (activity, effect), melanin production inhibitory effect, Maillard reaction inhibitory effect and antioxidant effect can be synergistically enhanced.

When the composition of the present invention is in the form of a cosmetic, its production method is not particularly limited as long as the effect of the ume extract is not significantly reduced, and can be appropriately produced by those skilled in the art.

In a particularly preferred embodiment, the production process includes a high-pressure emulsification treatment process. Thereby, the emulsified particles can be made fine and an emulsified state with high stability can be created. A ume extract that is relatively difficult to disperse can be sufficiently dispersed and stabilized, facilitating blending. In addition, the emulsified particles become finer, have better skin fit, increase permeability, and various effects of ume extract: elastase activity inhibitory effect, gelatinase activity inhibitory effect, collagenase activity inhibitory effect, hyaluronidase activity inhibitory effect, fiber A cosmetic product that synergistically enhances blast cell activation (activity, effect), melanin production inhibitory effect, Maillard reaction inhibitory effect, and antioxidant effect can be produced.

In a particularly preferred embodiment, the production process includes a deodorization process separately from or in combination with the high-pressure emulsification treatment process. According to the study by the present inventors, extracts from various parts of ume may have a characteristic odor derived from the raw material, and when a cosmetic composition is constituted, even in a relatively small amount, It may not be negligible to apply to the face. This tendency was particularly remarkable when roots were used.

The means for deodorization is not particularly limited as long as the effect of the ume extract is not significantly reduced, and an existing technique used for the same purpose can be applied. One typical method is to use activated carbon.

When the composition of the present invention is a health food, the ume extract is obtained from the ume extract obtained by the method described in Example 1 of the present invention (preferably, an extract of ume root using a ume shoot with a steamed ume branch). As an amount corresponding to the above, 0.01 to 30 g / 100 g, preferably 0.5 to 20 g / 100 g, more preferably 1 to 10 g / 100 g can be blended, and the ume extract obtained by the method described in Example 1 of the present invention The amount corresponding to the dry powder of the product (preferably, the dried powder of the extract of ume root with 95% ethanol) is 0.1 to 200 mg / 100 g, preferably 0.5 to 100 mg / 100 g, more preferably 5 to 50 mg / 100 g. Can be blended. Also in this case, each extract may be blended alone in the above amount, or may be blended in combination in the above amount. It is more preferable to combine them.

[Example 1: Preparation of ume part extract]
Each part of the ume was collected and lyophilized.

Each part of freeze-dried ume (all umes used in the examples, including those used below) were collected in this country) into purified water, 95% ethanol or steam distilled liquid. Then, it was soaked to 5% W / W and subjected to extraction at 30 ° C. for 4 days (3 days). After extraction, it was simply filtered with filter paper, and finally filtered aseptically with a 0.45 μm filter to prepare a ume part extract.

The ume part extract obtained as needed was powdered. The extract with purified water was freeze-dried and powdered. After removing the solvent from the ethanol extract once using an evaporator, an appropriate amount of purified water was added, freeze-dried, and powdered.

In addition, the steam distilled liquid of a ume fruit or a young branch was prepared as follows.

Ume fruit steam distillate:
Water vapor generated by boiling purified water was applied to 5 to 50% (w / w) of ume fruits, and the water vapor containing aroma components obtained was cooled.

Ume Wakae Steam Distillate:
Water vapor generated by boiling purified water was applied to 0.01 to 10% (w / w) of ume shoots to cool the water vapor containing aroma components obtained.

[Example 2: Evaluation of extract]
Test method:
(1) Elastase activity inhibitory effect test:
50 μL of an evaluation sample prepared using purified water on a 96 well plate, 35 μL of 0.1 M Tris-HCl buffer, 100 μL of 1 mM Suc-Ala-Ala-Ala-PNA, 0.25 U / mL (Wako Pure Chemical Industries, Ltd., 15 μL (derived from porcine pancreas) was added, and after reacting at 37 ° C. for 30 minutes, absorbance at 405 nm was measured with a microplate reader. The inhibition rate was calculated using the following formula with purified water as a control.

(2) DPPH radical scavenging effect test:
Microplate reader: 15 μL of evaluation sample prepared with purified water in 96 well plate, 15 μL of 0.2 M MES buffer 60 μL, 50 μL of 95% ethanol, 25 μL of 0.6 mM DPPH radical, reacted at 30 ° C. for 30 minutes The absorbance at 540 nm was measured. The elimination rate was calculated using the following formula with purified water as a control.

(3) SOD-like effect test:
Test sample 25 (L, 2 mM hypoxanthine 25 (L, 2 mM EDTA 25 (L, 0.5 mM NBT 25 (L, 0.1 M carbonate buffer 100 (L , 30 mU / mL xanthine oxidase (XOD) 50 (L was added, reacted at 37 ° C. for 30 minutes, and the absorbance at 570 nm was measured with a microplate reader. The elimination rate was calculated using the following formula with purified water as a control. .

(4) Hydrogen peroxide (H ₂ O ₂ ) erasing effect test:
25 μL of an evaluation sample prepared with purified water was added to a 96-well plate, and then 10 μL of 0.15 mM H ₂ O _{2 and} 25 μl of 0.1 M PIPES buffer (pH 7.0) were added and reacted at 37 ° C. for 20 minutes. . Immediately after the reaction, 172 μM DA-67 (180 μL) was added, ethanol (10 μL) was added, a color reaction was performed at 37 ° C. for 5 minutes, and the absorbance at 630 nm was measured with a microplate reader. The elimination rate was calculated using the following formula with purified water as a control.

(5) Hyaluronidase activity inhibitory effect test:
Evaluation sample prepared using purified water in a 96-well plate 12μL, 400U / mL hyaluronidase (SIGMA, cow testis) 12μL was mixed and incubated at 40 ° C for 20 minutes, 0.1mg / mL Compound 48/80 12μL And react at 40 ° C for 20 minutes, add 12 mg of 1 mg / mL potassium hyaluronate, and react at 40 ° C for 40 minutes.

After that, add 12μL of 0.4N NaOH, cool with ice water for 5 minutes to stop the reaction, add 12μL of 0.8M potassium borate solution, heat for 3 minutes with boiling water, and cool with ice water for 10 minutes. After adding 180 mg of 20 mg / mL p-DMAB and dispensing 180 μL to a 96-well plate, the mixture was reacted at 40 ° C. for 20 minutes, and the absorbance at 540 nm was measured with a microplate reader. The inhibition rate was calculated using the following formula with purified water as a control.

Calculation of hyaluronidase activity inhibitory effect:
The hyaluronidase activity inhibitory effect is calculated using the following formula.

(6) Collagenase activity inhibitory effect test:
60 μL of an evaluation sample prepared using purified water, 480 μL of 0.5 M pz-peptide adjusted with 0.1 M Tris-HCl buffer (pH 7.1, containing 20 mM CaCl ₂ ), and 50 CDU / mL collagenase adjusted with purified water ( 60 μL (from SIGMA, Clostridium histolyticum ) was added and reacted at 37 ° C. for 30 minutes. Immediately after the reaction, 1.2 mL of 2.5 mM citric acid was added, and then extracted with 5.0 mL of ethyl acetate. After centrifugation, the absorbance at 320 nm of the ethyl acetate layer was measured. Using purified water as a control, the inhibition rate of collagenase activity was calculated using the following formula.

(7) Fibroblast activation effect test:
Normal human dermal fibroblasts (NHDF) were seeded in a 96-well microplate at a density of 7 × 10 ⁴ cells / cm ² . As a seeding medium, an α-MEM medium containing 5% fetal bovine serum (FBS) was used and cultured in a CO ₂ incubator (5% CO ₂ , 37 ° C.) for 24 hours. After 24 hours, each sample was replaced with α-MEM medium containing 5% FBS, and cultured for 72 hours. After removing the supernatant, MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) was replaced with DMEM medium supplemented with 1.0 mg / ml and cultured for 4 hours. Thereafter, the produced formazan was extracted with acidic isopropanol, and the absorbance at wavelengths of 570 nm and 630 nm was measured with a plate reader. From the difference between the two measured values (absorbance at 570 nm−absorbance at 630 nm), the cell activation effect was evaluated using the following formula with the value in the case of addition of purified water as a control as the activation rate of 100%.

(8) B16 melanoma cell melanin production inhibitory effect test:
Prepare B16 melanoma cells in DMEM medium containing 10% fetal bovine serum (FBS) at 12 × 10 ⁴ cells / mL, seed the cell suspension at 0.5 mL / well in a 24-well plate (2 cm ² ), 37 ° C, Incubate under 5% CO ₂ . After 24 hours, the medium is replaced with 0.1 mg / mL theophylline and DMEM medium (10% FBS) supplemented with the evaluation sample. Two days after the medium change, the amount of melanin is washed twice with PBS, 1% TritonX-100 · 1N NaOH is added at 300 μL / well, and left at 50 ° C. for 1 hour to extract melanin. 250 μL of the extract is dispensed into a 96-well plate, the absorbance at 450 nm is measured with a plate reader, and the inhibition rate of melanin production is calculated using Equation 1. The number of viable cells is determined by the MTT method, and the cell viability is calculated by Equation 2. Evaluation is based on the data calculated using Equation 3 from the inhibition rate of melanin production and the cell viability.

(9) Gelatinase activity inhibitory effect test:
A 10% SDS polyacrylamide gel containing 0.2% gelatin is prepared without a comb by adding a pre-heated gelatin solution, and the MMP marker (manufactured by Primary Cell Co., Ltd.) is 1/4 and 1.5M Tris-HCl buffer (pH 8.8). Apply the sample diluted in step) and perform electrophoresis. After electrophoresis, wash repeatedly with 2.5% TritonX-100. Cut into strips with a length of 2 cm and a width of 0.4 cm from a position 5 mm below the boundary between the separation gel and the concentrated gel. Add 1350 μL of 0.05 M Tris-HCl buffer (5 mM CsCl ₂ , 5 μM ZnCl ₂ , pH 8.0) to the sample tube, and add 150 μL of the evaluation sample. Put the gels cut into strips one by one into the sample tube, and react for 48 hours while shaking at 37 ° C. After the reaction, stain and decolorize with CBB staining solution. The appearance after decolorization was determined visually by determining the disappearance of the MMP band, using as a comparative control the addition of purified water instead of the evaluation sample.

(10) Maillard reaction inhibitory effect test:
Add 0.5 μl ribose 40 μl, 50 mg / ml lysozyme 100 μl prepared with 800 μl 0.1M phosphate buffer (pH 7.4) and 800 μl 0.1M phosphate buffer (pH 7.4) to the sample tube, and adjust the concentration using purified water. 60 μL of the evaluation sample was added. The solution was sterilized by filtration using a 0.45 μm filter in a clean bench, and reacted for 7 days in a 40 ° C. incubator. After the reaction, electrophoresis was performed on SDS-PAGE, staining was performed with 0.1% Coomassie Brilliant Blue R-250, and after decolorization, the lysozyme dimer was visually determined. The determination was made by negative control using purified water instead of the evaluation sample, and the state when aminoguanidine sulfate was added was determined as a positive control.

result:
(1) SOD-like effect, DPPH radical scavenging effect, H ₂ O ₂ scavenging effect, elastase activity inhibitory effect The extract (stock solution) obtained by the method of Example 1 is made 100% and diluted with purified water for testing. Provided. In addition, the same test was performed on the extract of camellia plants, ascorbic acid (purified water), baicalin (5% DMSO / purified water). The table below shows the IC50 values for the test results and those for which effects were recognized. In the table, EtOH refers to ethanol (the same applies hereinafter).

In both Shirakaga and Bungo, high activity was observed in the shoots and roots. In particular, the SOD-like action effect, the DPPH elimination effect, and the H ₂ O ₂ elimination effect are remarkable for the young branch, and the SOD-like action effect, the DPPH radical elimination effect, and the elastase activity inhibition effect are remarkable for the root.

(2) Melanin production inhibitory effect The extract (stock solution) obtained by the method of Example 1 was taken as 100%, or the lyophilized product obtained by the method of Example 1 was redissolved in each solvent and used for the test. . A similar test was conducted for kojic acid (purified water). The test results are shown in the table below.

In the test with the extract, a high inhibitory effect was observed in the 95% ethanol extract of Shirakaga and Bungo young leaves and the 95% ethanol extract of Shirakaga leaves at the tested concentrations. The Shirakaga young leaf 95% ethanol extract also had a relatively high survival rate, and the desired effect (high inhibitory effect and did not reduce cell survival rate) was observed.

In the test with freeze-dried products, concentration-dependent suppression of melanin production was observed for any of the samples shown in the table. The purified water extract of Shirakaga root also had a relatively high survival rate, and the desired effect (high inhibitory effect and did not reduce the cell survival rate) was the highest.

(3) Fibrocyte activation effect
The lyophilized product obtained by the method of Example 1 was redissolved in each solvent and used for the test. Moreover, the same test was done also about sodium ascorbyl phosphate. The test results are shown in the table below.

There was a tendency for extracts from roots, bark, and branches to be highly effective over 0.5 mg / mL sodium ascorbyl phosphate.

(4) Collagenase activity inhibitory effect The lyophilized product obtained by the method of Example 1 was redissolved in each solvent and used for the test. Moreover, the same test was done also about the extract by the ginger family plant obtained by the same method. The test results are shown in the table below.

Equivalent effects were observed at lower concentrations than Ginger.

(5) Hyaluronidase activity inhibitory effect The lyophilized product obtained by the method of Example 1 was redissolved in each solvent and used for the test. A similar test was conducted for sodium cromoglycate. The test results are shown in the table below.

(6) Gelatinase activity inhibitory effect The lyophilized product obtained by the method of Example 1 was redissolved in each solvent and used for the test. Purified water and EDTA (purified water) were used as comparative controls. The test results are shown in FIG.

Inhibition of gelatinase activity was observed in the root ethanol extracts of Shirakaga and Bungo.

(7) Maillard reaction inhibitory effect The lyophilized product obtained by the method of Example 1 was redissolved in each solvent and subjected to the test. A similar test was also conducted on aaminoguanidine sulfate. The test results are shown in FIG.

High effect was observed in purified water extract and ethanol extract of each root of Shirakaga and Bungo. About Wakaeda, the effect was recognized in the purified water extract and ethanol extract of Bungo.

[Example 3: activated carbon treatment]
To the extract obtained by the method of Example 1, 10% (w / w) of granular white birch WH2c8 / 32-3 (Nippon EnviroChemicals Co., Ltd.), more specifically, activated carbon / (activated carbon + extracted liquid) ) × 100 = 10 (% w / w) was immersed and stirred well, and then allowed to stand at room temperature (20 ° C.) for 2 days. After immersion, filtration was performed to remove activated carbon in the extract. Filter sterilization was performed with a 0.45 μm filter, and a sensory test was conducted for odor.

The results are shown in the table below.

In addition, the elastase activity inhibitory effect test was carried out by the method described in Example 2 for the product after the activated carbon treatment, and compared with the result for the product before the treatment. In the table, “EtOH extraction” refers to extraction with 95% ethanol (the same applies to the following examples unless otherwise noted).

By the activated carbon treatment, the odor derived from the material was reduced, and the activity was not significantly reduced, making it more suitable as a raw material to be added to cosmetics.

[Example 4: Evaluation of use]
A serum containing ume extract was manufactured and used and evaluated.

(Manufacture of serum containing ume extract)
The aqueous phase A having the composition shown in the following table was heated and dissolved at 85 ° C., B was added, and the mixture was stirred. The oil phase C was heated and dissolved at 100 ° C. The oil phase C was added to the aqueous phase A and B mixture, heated to 85 ° C., and emulsified with an emulsifier. Thereafter, it was cooled to 35 ° C. and subjected to high-pressure treatment (220 MPa × 5 pass) to obtain a pre-treatment liquid for serum.

Next, the aqueous phase A shown in the table below was heated and dissolved at 85 ° C. and cooled to 37 ° C. Each component of B was added to aqueous phase A, and C (the essence pretreatment product obtained in the above step (a)) was added to the mixture of aqueous phases A and B, and dispersed uniformly to obtain a essence. .

(Manufacture of comparative serum)
On the other hand, aqueous phase A having the composition shown in the table below was dissolved by heating to 75 ° C., cooled to 35 ° C., added with aqueous phase B, and stirred. Finally, C was added in small portions and stirred to obtain a comparative cosmetic liquid.

The sensory test of the manufactured cosmetics was conducted by 20 panelists (general women in their 20s and 60s).

】 After taking an appropriate amount (0.2-0.5ml) of the above-mentioned essence, it was applied to the right and left faces in half on the skin after washing. About 1 month, it was continuously used almost twice a day, and the results compared with the comparative example were judged on the following 7 criteria.

The cosmetic liquids of the examples were superior in all items as compared to the comparative examples.

[Example 5: Preparation of cosmetics, etc.]
(Manufacture of lotion 1)
In the following table, heat to 75 ° C and dissolve with stirring.
After cooling to 35 ° C., a lotion was obtained.

(Manufacture of lotion 2)
Process (a) Pre-treatment for lotion and emulsion:
Dissolve water phase A and oil phase B in the composition shown in the table below.
Add oil phase B to water phase A and emulsify with an emulsifier.
The emulsion is heated to 40 ° C. and C is added and stirred.
Thereafter, it was cooled to 35 ° C. and subjected to high-pressure treatment (220 MPa × 5 pass) to obtain a pre-treatment product for lotion 2.

Step (b):
The aqueous phase A having the composition shown in the table below is heated to 80 ° C. and dissolved.
Cool to 40 ° C., add B (pretreatment for lotion / milky emulsion obtained in the above step (a)) and stir.
After cooling to 35 ° C., lotion 2 was obtained.

(Manufacture of emulsion)
The aqueous phase A having the composition shown in the table below is heated to 85 ° C. and dissolved.
Add B (the pre-treatment for the lotion / milky emulsion obtained in step (a) of lotion 2) and stir.
Heat and dissolve only C.
C is added to the mixture of A and B, heated to 85 ° C. and emulsified with an emulsifier. The emulsion was cooled to 35 ° C. to obtain an emulsion.

(Cream production)
The water phase A and the oil phase B having the composition shown in the table below are heated to 80 ° C. and dissolved.
Add B to A and emulsify with an emulsifier.
Cooled to 35 ° C. to obtain a cream.

(Manufacture of multilayer cream)
Step (a):
The water phase A and the oil phase B having the composition shown in the table below are heated to 80 ° C. and dissolved.
Add B to A and emulsify with an emulsifier.
The emulsion was subjected to high-pressure emulsification (25 ° C., 220 MPa, 5 pass) to obtain a multilayer cream pretreatment product.

Step (b):
The water phase A and the oil phase B having the composition shown in the table below are heated to 80 ° C. and dissolved.
Add B to A and emulsify with an emulsifier.
C (the multilayer cream pretreatment product obtained in step (a) above) is added to the emulsion and stirred, and D is added and further stirred. Cooled to 35 ° C. to obtain a multilayer cream.

(Health food production)
The ingredients obtained by freeze-drying all the ingredients shown in the table below were mixed to obtain a food.

(Manufacture of soap)
Heat all ingredients in the following table until uniform and stir to dissolve.
A face washing soap was obtained after filtration, cooling, solidification, cutting, and drying.

(Manufacture of lip base)
Oil phase A having the composition shown in the following table is heated to 100 ° C. and dissolved.
Add B (the essence pretreatment obtained in step (a) of the essence) and stir.
Defoam and pour into a mold that has been set to 30 ° C. at 80 ° C. and cool.
Removed from the mold to get a lip base.

[Example 6: Test on blending amount of emulsifier]
[Test 1]
Test method:
Add ume extract (dry powder of 95% ethanol extract of Shirakaga ume root) to purified water at a concentration of 0.1 mg / mL (approximately the same as 0.1 mg / g), and add emulsifier (PEG-60 hydrogenated castor oil). The test samples were mixed at the concentrations shown in the table below. In addition, the sample was heated at 80 ° C. to 95 ° C., then mixed by vortexing for 1 minute or longer, and photographed to confirm the presence or absence of precipitation.

Test results:
The results are shown in the table below.

A photograph of the same sample taken at different angles is shown in FIG. It was found that the ume extract was dissolved by the addition of the emulsifier.
[Test 2]
Test method:
Ume extract (dry powder of Shirakaga ume root 95% ethanol extract) was dissolved in purified water at a concentration of 0.25 wt%, and the same emulsifier used in Test 1 was mixed at the following concentrations to prepare a test sample. . The sample was heated at 80 ° C. to 95 ° C., then mixed by vortexing for 1 minute or more and cooled to room temperature. The cooled sample was centrifuged at 12000 rpm for 2 minutes and photographed to confirm the presence or absence of precipitation.

result:

Although H-5 was dissolved, it was highly viscous and unsuitable for cosmetic production (Figure 4).
[Test 3]
Test method:
A ume extract (dry powder of Shirakaga ume root 95% ethanol extract) was dissolved in purified water at a concentration of 0.25 wt%, and an emulsifier was mixed at the following concentration to prepare a test sample. As the emulsifier, four kinds of lysolecithin, PEG-60 hydrogenated castor oil, hydrogenated lecithin, and soybean sterol were blended at the same ratio as in Example [Table 9] of the present specification. The sample was heated at 80 ° C. to 95 ° C., then mixed by vortexing for 1 minute or more and cooled to room temperature. The cooled sample was centrifuged at 500 rpm for 2 minutes and photographed to confirm the presence or absence of precipitation.

Test results:
The results are shown in the table below.

When the emulsifier was less than 0.01%, it was found that the plum extract cannot be dissolved.

Claims

Selected from the group consisting of water, steam, purified water, mineral water, ethanol, propylene glycol, and 1,3-butylene glycol from one or more sites selected from roots, branches and bark of ume A cosmetic composition comprising 0.05 to 250 mg / 100 g of an extract of one or more solvents as a dry powder and 0.015 to 5 g / 100 g of an emulsifier.
2. The composition of claim 1, wherein the emulsifier is lysolecithin, PEG-60 hydrogenated castor oil, hydrogenated lecithin, soybean sterol, or any combination thereof.
2. The composition according to claim 1, comprising a water extract and an ethanol extract of ume root.
Elastase activity and / or hyaluronidase comprising applying an extract of ume root ethanol and / or water to the skin of the subject, thereby inhibiting elastase activity and / or hyaluronidase activity in the skin of the subject A method of treating a skin condition that is improved by inhibition of activity.
5. The method according to claim 4, which is a method for suppressing the progress of aging.
6. The method according to claim 4 or 5, which further comprises a step of suppressing Maillard reaction or melanin production in the subject's skin, which is also a method for whitening the skin.