KR102040245B1

KR102040245B1 - Skin moisture and anti-aging cosmetic composition with the extract of Aloe Vera by Chojeong mineral water

Info

Publication number: KR102040245B1
Application number: KR1020170052353A
Authority: KR
Inventors: 박성민; 이정노; 김지은; 박진실
Original assignee: 주식회사 코씨드바이오팜
Priority date: 2017-04-24
Filing date: 2017-04-24
Publication date: 2019-11-07
Also published as: KR20180119189A

Abstract

The present invention relates to a cosmetic composition for moisturizing or preventing skin aging, wherein the cosmetic composition of the present invention containing an extract extracted by adding primary mineral water to aloe vera leaves exhibits an excellent skin moisturizing effect and anti-aging effect.

Description

Skin moisturizing or anti-aging cosmetic composition containing extract extracted by adding primary mineral water to aloe vera leaves {Skin moisture and anti-aging cosmetic composition with the extract of Aloe Vera by Chojeong mineral water}

The present invention relates to a skin moisturizing or anti-aging cosmetic composition, and relates to a skin moisturizing or anti-aging cosmetic composition containing an extract extracted by adding the primary mineral water to aloe vera leaves.

Skin is an organ that functions as a protective film to protect the living body from the external environment, and prevents the loss of biological components such as water or electrolyte in the human body and prevents harmful substances from entering the human body from the outside. The skin is divided into three layers of epidermis, dermis and subcutaneous tissue in order from the outside. Among them, the epidermal layer plays an important role in preventing moisture evaporation inside the human body.

Generally, the moisture content of the skin is about 70% in the dermal layer, but decreases toward the epidermal layer, which is about 10% to 30% in the keratinocyte layer.When the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and loses the protective function of the body. Aging occurs. In addition, the water supplied from the dermal layer is mainly moved to the upper stratum corneum by passive diffusion and finally discharged to the outside. This is called Trans Epidermal Water Loss (TEWL), and it is known that it is the sebaceous component and the epidermal lipid that are the lipid membrane components of the keratinocyte layer to maintain the appropriate level of transdermal moisture loss.

Therefore, it is very important to maintain the moisture content of the keratinocyte layer of the skin properly. To this end, cosmetics are used in addition to ingredients similar to sebum, moisturizers such as NMF components or polyols. For example, glycerin, solitol, and the like having three or more hydroxyl groups (OH groups) as water-soluble polyols exhibit excellent moisturizing power. However, it is very sticky and makes you feel uncomfortable, and hydroxyglycol (OH group) having two propylene glycol, 1,3-butylene glycol, etc. may cause side effects of the skin. In addition, other natural moisturizing factors, such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate, urea, etc., have a strong electrolytic property and thus impair the emulsion stability of the cosmetic. In addition, amino acids, collagen, elastin and the like also have a moisturizing ability, but its moisturizing ability is known to be limited.

On the other hand, water is the source of life and occupies more than 70% of our body. Water in the human body maintains cell morphology, enhances metabolism, facilitates circulation of blood and tissue fluids, dissolves nutrients, absorbs and transports them, and supplies them to cells that need nutrients. Maintains neutral to weak alkaline. In addition, the body wastes unnecessary waste in the body, and serves to maintain body temperature by dissipating heat in the body.

Water is one of the most important ingredients in cosmetic compositions. In general, water used in cosmetics is purified water purified by distillation, ion exchange and ultrafiltration. In recent years, consumers' awareness of the function and importance of water has increased greatly, and with the modern man's longing for uncontaminated pure nature, interest in water that can replace purified water has increased. Recently, a lot of cosmetics using natural raw materials have been developed, and research on water having a special function in place of purified water is being actively conducted.

On the other hand, mineral water is water containing trace amounts of minerals such as calcium, magnesium and potassium and is also called mineral water. It is a spring that springs out from the ground and contains a large amount of gaseous or solid matter. The hot spring is 25 ℃ or higher in Cheonwon, and the cold spring is called the hot spring.

On the other hand, Chojeong mineral water is a natural mineral water from Chojeong-ri, Cheongwon-gun, Chungcheongbuk-do. Rich in minerals beneficial to the human body, it is an international sparkling water with a 600-year history called "Oriental Mysterious Water."

Republic of Korea Patent Publication No. 10-2011-0110995 (published date 2011.10.10), a cosmetic composition comprising a primary mineral water and a mask pack comprising the same is described. Republic of Korea Patent Publication No. 10-2012-0103851 (published date 2012.09.20), after the addition of moon-root to the mineral water of alkaline water obtained by electrolysis of mineral water, Saccharomyces cerevisisae KCTC 7904 Contains a fermented soybean extract obtained by inoculating fermentation as an active ingredient, has been described a cosmetic composition excellent in skin moisturizing effect, antioxidant effect, whitening effect.

In the present invention, to determine whether the primary mineral water is applicable to cosmetics, and to develop and provide a cosmetic composition utilizing this.

The present invention provides a skin moisturizing cosmetic composition comprising an extract extracted by adding primary mineral water to aloe vera leaves.

In the cosmetic composition for moisturizing the skin of the present invention, the extract may be extracted by adding 2.5 ~ 3.5 parts by weight of primary mineral water to 1 part by weight of aloe vera leaves in aloe vera leaves.

The present invention provides a cosmetic composition for preventing skin aging, comprising extract extracted by adding primary mineral water to aloe vera leaves.

In the cosmetic composition for preventing skin aging of the present invention, the extract may be preferably extracted by adding 2.5 to 3.5 parts by weight of primary mineral water to 1 part by weight of aloe vera leaves.

The extract extracted by adding ultra mineral water to the aloe vera leaf of the present invention is excellent in skin moisturizing ability, it can be used as a raw material for skin moisturizing cosmetics.

In addition, the extract extracted by adding primary mineral water to the aloe vera leaf of the present invention is excellent in antioxidant capacity, it can be used as a raw material of skin anti-aging cosmetics.

In addition, since the present invention uses aloe vera leaf and primary mineral water, which are natural materials, the present invention is harmless to humans and has excellent safety.

1 is a photograph qualitatively confirming the aquaporin 3 (AQP-3) expression of the aloe vera leaf extract of the present invention.
Figure 2 is a bar graph confirming aquaporin 3 (AQP-3) expression of the aloe vera leaf extract of the present invention quantitatively.

Chojeong mineral water used in the present invention means mineral water that is eluted from Chojeong-ri area, Naesu-eup, Cheongwon-gun, Chungcheongbuk-do, Korea, is classified as cold spring. According to the 1974 Chungbuk carbonate spring survey, the water temperature of Chojeong mineral spring water is 13-15 ℃. Also, Primary mineral water is dissolved, which is contained in the mineral water 1 kg collected in the water with regard to the content of the acid eluted at Chapter mineral, i.e. that does not contain CO ₂ teller state to a gaseous state, mineral jeongjeo (鑛泉井底) It has been reported that the concentration of free CO _{2 in the} state is up to 2460 mg / L. In addition, looking at the mineral content of the primary mineral water is shown in Table 1, the appearance is known as a colorless transparent liquid.

ingredient content Calcium (mg / 100g) 2.72 Magnesium (mg / 100 g) 2.05 Phosphorus (mg / 100 g) 0.13 Potassium (mg / 100 g) 2.13 Sodium (mg / 100 g) 2.47 Zinc (mg / 100 g) 0.02 Iron (mg / 100 g) 0.20 Manganese (mg / 100 g) 0.004 Copper (mg / 100 g) 0.02 Selenium (μ / 100 g) 1,325.76

According to the component analysis table, primary mineral water contains 1,325.76 ㎍ / 100 g of selenium (Selenium, Se, Atomic Number 34), which is known as an antioxidant as an essential trace inorganic substance in the body.

On the other hand, Aloe Vera (Aloe Vera) used in the present invention is a perennial plant of the genus Liliaceae, which is a kind of aloe, and is widely grown in the tropical and temperate regions today and is cultivated in other regions. Aloe vera leaves are long locusts with sharp tips, slightly sharp spines at the edges of leaves, fleshy, without petioles, displaced from the stem. Polysaccharides of aloe vera leaves are known to be effective in strengthening immune function and softening rough and tired skin, and have an excellent effect on skin calming and skin aging.

On the other hand, in the present invention, using the above-mentioned primary mineral water as a solvent, extract the aloe vera leaf to prepare an extract, and develop and provide a cosmetic moisturizing cosmetic composition containing the same.

According to the experiment of the present invention, the aloe vera leaf primary mineral water extract of the present invention is based on a high water holding capacity (water holding capacity), to enhance the skin moisturizing power, prevent transdermal moisture loss (TEWL), and It has been confirmed to keep the skin clear.

In addition, in the present invention, in order to confirm the skin moisturizing effect, the degree of aquaporine expression was examined. Aquaporine selectively passes only water molecules and is a major intrinsic protein family present in all cell membranes, and has a mechanism of increasing skin moisture absorption. In mammals, 13 kinds are known, of which aquaporin 3 is expressed in keratinocytes of the skin, and the absorption and migration of water and glycerol through aquaporin 3 prevents the loss of moisture in the cells, thereby moisturizing and It is known to increase elasticity.

In the present invention, it was confirmed that the expression of aquaporin was increased when extracted by the addition of primary mineral water than the extract with purified water added to the aloe vera leaf. In view of these results, the cosmetic composition of the present invention was confirmed that the expression of aquaporin to increase the water absorption of the skin cells can increase the skin moisturizing power.

In addition, the present invention provides a cosmetic composition for preventing skin aging, characterized in that it comprises an extract extracted by adding primary mineral water to aloe vera leaves, according to the experiment of the present invention, the content of polyphenols having excellent antioxidant capacity Extraction using primary mineral water was higher than extraction using purified water.

On the other hand, in the skin moisturizing or skin anti-aging cosmetic composition of the present invention, the extract may be extracted by adding 2.5 to 3.5 parts by weight of primary mineral water to 1 part by weight of aloe vera leaves.

In addition, the aloe vera leaf in the present invention is preferably used after a sufficiently washed and pulverized, it is preferable to use after finely made using a 100 mesh sieve. In addition, aloe vera leaf may be used lyophilized.

Hereinafter, the present invention will be described in more detail in the following examples. However, the scope of the present invention is not limited only to the following examples, but includes all modifications of equivalent technical spirit.

[ Example One : Invitation Preparation of Aloe Vera Leaf Extract with Mineral Water]

Aloe vera leaves were selected as a sample and washed sufficiently, and then about 1 kg of aloe vera leaves were cut to a size of 5 cm. Then, 3 kg of primary mineral water was added thereto, stirred for 5 hours, and filtered using a 400-mesh sieve. After filtering, an extract was obtained and used in the following experiment.

[ Comparative example One : Purified water Preparation of Added Aloe Vera Leaf Extract]

Aloe vera leaf extract was obtained in the same manner as in Preparation Example 1, except that purified water was used. The extract obtained by the above method was confirmed to have the following characteristics.

Total Polyphenols (Poline-Siocalto; Gallic Acid Equivalent)) Example 1 Comparative Example 1 38.71 mg / g 0.71 mg / g

As shown in the results of Table 2, Example 1 was confirmed to have a significantly higher total polyphenol content compared to Comparative Example 1. Therefore, in the case of Example 1 of the present invention, it could be evaluated that the antioxidant ability is excellent.

[ Example 2 : Invitation Preparation of Hyaluronic Acid Solution Added Mineral Water]

Hyaluronic acid is theoretically capable of attracting hundreds of times its own weight and, in fact, about 80 times its own weight, making it one of the best moisturizing agents. The hyaluronic acid is a substance contained in a certain amount of the human body to deliver nutrients to the cells, and acts as a lubricant, and prevents water evaporation of the skin at the same time to absorb the moisture to keep the skin properly moisture.

In this embodiment, hyaluronic acid sodium salt (Sodium Hyaluronate) was added to the primary mineral water in 0.5% content to prepare a hyaluronic acid solution.

[ Comparative example 2 : Purified water Preparation of Added Hyaluronic Acid Solution]

A hyaluronic acid solution was prepared in the same manner as in Example 2, except that purified water was used.

[ Experimental Example One : DPPH method Antioxidant Effect Measurement Experiment]

Example 1 and Comparative Example 1 It was intended to confirm the free radical scavenging action of the raw material. The 1,1-Diphenyl-2-pycryl-Hydrazyl (DPPH) method, which is used as a primary method for confirming free radical scavenging action due to the presence of relatively stable free radicals, was used.

After treating the aloe vera leaf extract in each concentration (300, 150, 75, 37.5, 18.75 ppm) in Example 1 and Comparative Example 1 to prepare each sample, the DPPH solution dissolved in 2 mM ethanol solution The sample is added to a total volume of 1 ml.

Each sample was left at 37 ° C. for 30 minutes, and then transferred to 96 wells, where the absorbance was measured using a 517 nm ELISA reader.

Samples used were aloe vera leaf ultra mineral water extract prepared in Example 1 and Comparative Example 1, aloe vera leaf purified water extract, vitamin C was used as a positive control. The free radical scavenging action (%) was calculated by the following Equation 1, and the results are shown in Table 3 below.

[Equation 1]

Free radical scavenging effect (%) = 100-B / A × 100

A: Absorbance of control wells without sample

B: Absorbance of Experimental Wells Treated with Samples

Sample name Treatment Concentration (ppm) Free radical scavenging rate (%) Example 1 Comparative Example 1 Vitamin c Aloe vera leaf
extract 300 91.54 75.41 93.22 150 86.72 61.64 85.33 75 72.30 49.88 51.23 37.5 63.49 30.29 35.45 18.75 52.21 26.54 31.23

As shown in the results of Table 3, when the aloe vera leaf extracted with primary mineral water (Example 1), it was confirmed that there is a higher active oxygen removal effect than when extracted with purified water (Comparative Example 1).

[ Experimental Example 2 measurement of water holding capacity]

This Experimental Example is a solution in which hyaluronic acid (HA) is added under laboratory conditions to determine the degree of water holding capacity of the extract extracted from aloe vera leaves using the primary mineral water obtained in Example 1 Was used as a comparative sample to measure the water holding capacity (water holding capacity).

In a 10 L desiccator containing 100 g of calcium chloride (CaCl ₂ ), 10 g of each of Examples 1 and 2 and Comparative Examples 1 and 2 were placed in a petri dish and left for 3 hours, after which the amount of evaporated water was measured. Water holding capacity was measured.

sample Initial weight (g) Weight after 3 hours (g) Example 1 10.0 7.5 Example 2 10.0 7.5 Comparative Example 1 10.0 3.1 Comparative Example 2 10.0 2.9

As described above, it was found that Examples 1 and 2 had better binding strength with water than Comparative Examples 1 and 2, so that the amount of evaporated water was smaller.

[ Experimental Example 3: Invitation Mineral water Aloe vera leaf Determination of Skin Moisturizing Effect of Extracts- Aquaporin ( Aquaporin 3) Confirmation of expression]

Example 1, Comparative Example 1 was used to measure the moisturizing effect by increasing the expression of aquaporin 3 (aquaporin 3) to increase the water absorption of the skin cells by the following method. Dexamethasone was used as a positive control.

Human keratinocytes were seeded in a 6 well cell culture dish at a density of 4 × 10 ⁵ and then incubated in 37 ° C., 5% CO ₂ incubator for 24 hours. Two samples (30 μg / ml of Example 1, 100 μg / ml) and 1 ppm of dexamethasone were added as a positive control and incubated for 24 hours, and cells were recovered by using trizol (RNA iso, DAKARA, Japan). RNA was isolated and quantified RNA at 260 nm using nanodrop, and cDNA was synthesized in an amplifier using 2 μg of RNA each (C1000 Thermal Cycler, Bio-Rad, USA).

Finally, real-time polymerase chain reaction was carried out in a real-time PCR machine using a mixture of the synthesized cDNA with the target protein aquaporin 3 primer and the cyanine dye cyberrin (SYBR Green supermis, Applied Biosystems, USA). The expression level of the aquaporin 3 gene was evaluated. The expression level of the gene was finally analyzed through the correction for the β-actin gene.

As a result of the experiment, as shown in Figures 1 and 2 confirmed the expression of aquaporin 3 of two treated samples, Comparative Example 1 was poor expression of aquaporin 3 Example 1 is similar to dexamethasone treated with a positive control group The effect of increasing the expression of aquaporin 3 was confirmed.

[ Formulation example 1 to 2: Invitation Skin moisturizing containing extract extracted with mineral water Cosmetics Preparation of the composition]

In Formulation Examples 1 to 2, cosmetics were prepared using the aloe vera leaf extract added with the primary mineral water obtained in Example 1 and the aloe vera leaf extract added with the purified water obtained in Comparative Example 1.

The cosmetics used in the experiments were in the form of a cream, the composition of which is shown in Table 5. First, the b) phase shown in Table 5 was heated and stored at 70 degreeC. A) phase was added thereto, followed by preliminary emulsification to uniformly emulsify with a homomixer, and then gradually cooled to prepare a cream (Formulation Examples 1 to 2).

Raw material Formulation Example 1 Formulation Example 2 end Stearyl alcohol 8 8 Stearic acid 2 2 Stearic cholesterol 2 2 Squalane 4 4 2-octyldodecyl alcohol 6 6 Polyoxyethylene (25 mol addition)
Alcohol ester 3 3 Glyceryl Monostearic Acid Ester 2 2 I Example 1 5 - Comparative Example 1 - 5 Purified water Until 100 Until 100

Note) Unit: weight%

[ Experimental Example 4: skin Moisturizing effect Measurement experiment]

In this experimental example was to check the skin moisturizing effect of each cosmetic composition prepared in Formulation Examples 1 to 2.

Formulation Example 1 and Formulation Example 2 were applied to the lower chest, and the moisturizing ability (△ Hydration) was evaluated using a moisture meter (Corneometer, CM 820) before and after application for about 20 minutes. .

division Formulation Example 1 Formulation Example 2 Hydration 18 8

Note) Unit:%

As a result of measuring the skin moisturizing effect, it was found that the moisturizing effect was excellent in the lower part of the experimenter who applied the cream containing the aloe vera leaf extract added with primary mineral water (Formulation Example 1).

[ Experimental Example 5: Transdermal moisture loss Measurement experiment]

In the present experimental example, the transdermal moisture loss amount (TEWL: Trans-Epidermal Water Loss) of the skin moisturizing cosmetic composition prepared in Formulation Example 1 and Formulation Example 2 was attempted.

For each 40 women in their 20s and 40s, apply each sample on their right and left faces and take a 1 cm to 3 cm right side of their eyes, 1 hour, 2 hours, 4 hours, 6 hours before and after application. Percutaneous moisture loss was evaluated using the average value measured three times using Tewameter, TM300, Courage and Khazaka Electronic Co., Germany. Table 7 below is a comparison of the measured percutaneous moisture loss of the experimenter.

division 1 hours 2 hours 4 hours 6 hours Formulation Example 1 16.9 15.6 14.4 13.8 Formulation Example 2 16.2 13.4 9.9 7.3

Note) Unit: g / m²

As a result of the measurement of transdermal moisture loss, it was found that the amount of transdermal moisture loss was reduced in the facial skin of the experimenter who applied the cream containing Aloe vera leaf extract added with primary mineral water (Formulation Example 1) as shown in Table 7 above.

Claims

It includes a process of obtaining aloe vera leaf extract by adding 2.5 ~ 3.5 parts by weight of primary mineral water to 1 part by weight of aloe vera leaf to extract aloe vera leaf,
The primary mineral water is a manufacturing method of the skin moisturizing cosmetic composition, characterized in that the mineral water containing calcium, magnesium, phosphorus, potassium, sodium, zinc, iron, manganese, copper and selenium.

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It includes a process of obtaining aloe vera leaf extract by adding 2.5 ~ 3.5 parts by weight of primary mineral water to 1 part by weight of aloe vera leaf to extract aloe vera leaf,
The primary mineral water is a method for producing an antioxidant cosmetic composition, characterized in that the mineral water containing calcium, magnesium, phosphorus, potassium, sodium, zinc, iron, manganese, copper and selenium.

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