KR20140147949A - Antioxidant composition comprising aloe vera extracts - Google Patents
Antioxidant composition comprising aloe vera extracts Download PDFInfo
- Publication number
- KR20140147949A KR20140147949A KR20130070759A KR20130070759A KR20140147949A KR 20140147949 A KR20140147949 A KR 20140147949A KR 20130070759 A KR20130070759 A KR 20130070759A KR 20130070759 A KR20130070759 A KR 20130070759A KR 20140147949 A KR20140147949 A KR 20140147949A
- Authority
- KR
- South Korea
- Prior art keywords
- aloe vera
- extract
- antioxidant composition
- aloe
- months
- Prior art date
Links
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 35
- 239000000203 mixture Substances 0.000 title claims abstract description 31
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 22
- 235000014104 aloe vera supplement Nutrition 0.000 title claims description 48
- 235000011399 aloe vera Nutrition 0.000 claims abstract description 48
- 235000002961 Aloe barbadensis Nutrition 0.000 claims abstract description 35
- 244000186892 Aloe vera Species 0.000 claims abstract description 35
- 239000000284 extract Substances 0.000 claims abstract description 22
- 208000019423 liver disease Diseases 0.000 claims abstract description 10
- 208000019425 cirrhosis of liver Diseases 0.000 claims abstract description 8
- 208000006454 hepatitis Diseases 0.000 claims abstract description 8
- 231100000283 hepatitis Toxicity 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 13
- 239000006228 supernatant Substances 0.000 claims description 10
- 229930003935 flavonoid Natural products 0.000 claims description 7
- 235000017173 flavonoids Nutrition 0.000 claims description 7
- 150000002215 flavonoids Chemical class 0.000 claims description 7
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 6
- 235000013824 polyphenols Nutrition 0.000 claims description 6
- 239000004480 active ingredient Substances 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 4
- 239000000654 additive Substances 0.000 claims description 3
- 230000000996 additive effect Effects 0.000 claims description 3
- 239000003937 drug carrier Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 235000012041 food component Nutrition 0.000 claims description 3
- 235000013376 functional food Nutrition 0.000 claims description 3
- 230000036541 health Effects 0.000 claims description 3
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 230000036542 oxidative stress Effects 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 8
- 230000006378 damage Effects 0.000 abstract description 3
- 206010016654 Fibrosis Diseases 0.000 abstract 1
- 230000003064 anti-oxidating effect Effects 0.000 abstract 1
- 230000007882 cirrhosis Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 17
- 239000000243 solution Substances 0.000 description 17
- 235000006708 antioxidants Nutrition 0.000 description 15
- 241001116389 Aloe Species 0.000 description 13
- 230000002292 Radical scavenging effect Effects 0.000 description 11
- 239000003642 reactive oxygen metabolite Substances 0.000 description 11
- 210000005229 liver cell Anatomy 0.000 description 8
- 239000000523 sample Substances 0.000 description 7
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- LXEKPEMOWBOYRF-QDBORUFSSA-N AAPH Chemical compound Cl.Cl.NC(=N)C(C)(C)\N=N\C(C)(C)C(N)=N LXEKPEMOWBOYRF-QDBORUFSSA-N 0.000 description 5
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 5
- 239000002953 phosphate buffered saline Substances 0.000 description 5
- 102100021569 Apoptosis regulator Bcl-2 Human genes 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 101000971171 Homo sapiens Apoptosis regulator Bcl-2 Proteins 0.000 description 4
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 4
- 230000006907 apoptotic process Effects 0.000 description 4
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 238000002376 fluorescence recovery after photobleaching Methods 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 4
- 102000007469 Actins Human genes 0.000 description 3
- 108010085238 Actins Proteins 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 125000000217 alkyl group Chemical group 0.000 description 3
- 102000055102 bcl-2-Associated X Human genes 0.000 description 3
- 108700000707 bcl-2-Associated X Proteins 0.000 description 3
- 230000030833 cell death Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000012086 standard solution Substances 0.000 description 3
- AZKSAVLVSZKNRD-UHFFFAOYSA-M 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Chemical compound [Br-].S1C(C)=C(C)N=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 AZKSAVLVSZKNRD-UHFFFAOYSA-M 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- RNRMWTCECDHNQU-XYOKQWHBSA-N N-tert-butyl-1-(1-oxidopyridin-1-ium-4-yl)methanimine oxide Chemical compound CC(C)(C)[N+](\[O-])=C/C1=CC=[N+]([O-])C=C1 RNRMWTCECDHNQU-XYOKQWHBSA-N 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- GLEVLJDDWXEYCO-UHFFFAOYSA-N Trolox Chemical compound O1C(C)(C(O)=O)CCC2=C1C(C)=C(C)C(O)=C2C GLEVLJDDWXEYCO-UHFFFAOYSA-N 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 230000022131 cell cycle Effects 0.000 description 2
- 230000003833 cell viability Effects 0.000 description 2
- 230000001120 cytoprotective effect Effects 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical class O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 2
- -1 iron (Fe) ion Chemical class 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 230000005976 liver dysfunction Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 description 1
- VQVUBYASAICPFU-UHFFFAOYSA-N (6'-acetyloxy-2',7'-dichloro-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(Cl)=C(OC(C)=O)C=C1OC1=C2C=C(Cl)C(OC(=O)C)=C1 VQVUBYASAICPFU-UHFFFAOYSA-N 0.000 description 1
- KMVWNDHKTPHDMT-UHFFFAOYSA-N 2,4,6-tripyridin-2-yl-1,3,5-triazine Chemical compound N1=CC=CC=C1C1=NC(C=2N=CC=CC=2)=NC(C=2N=CC=CC=2)=N1 KMVWNDHKTPHDMT-UHFFFAOYSA-N 0.000 description 1
- YDQWDHRMZQUTBA-UHFFFAOYSA-N Aloe emodin Chemical compound C1=CC=C2C(=O)C3=CC(CO)=CC(O)=C3C(=O)C2=C1O YDQWDHRMZQUTBA-UHFFFAOYSA-N 0.000 description 1
- HKIKAXXIWJHWLY-ZIIYPAMZSA-N Aloesin Chemical compound C=12OC(CC(=O)C)=CC(=O)C2=C(C)C=C(O)C=1[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HKIKAXXIWJHWLY-ZIIYPAMZSA-N 0.000 description 1
- HKIKAXXIWJHWLY-QEVGBQTESA-N Aloesin Natural products O=C(CC=1Oc2c([C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)c(O)cc(C)c2C(=O)C=1)C HKIKAXXIWJHWLY-QEVGBQTESA-N 0.000 description 1
- AFHJQYHRLPMKHU-XXWVOBANSA-N Aloin Natural products O=C1c2c(O)cc(CO)cc2[C@H]([C@H]2[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O2)c2c1c(O)ccc2 AFHJQYHRLPMKHU-XXWVOBANSA-N 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 206010019837 Hepatocellular injury Diseases 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 208000001145 Metabolic Syndrome Diseases 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 239000006180 TBST buffer Substances 0.000 description 1
- LXEKPEMOWBOYRF-UHFFFAOYSA-N [2-[(1-azaniumyl-1-imino-2-methylpropan-2-yl)diazenyl]-2-methylpropanimidoyl]azanium;dichloride Chemical compound Cl.Cl.NC(=N)C(C)(C)N=NC(C)(C)C(N)=N LXEKPEMOWBOYRF-UHFFFAOYSA-N 0.000 description 1
- 201000000690 abdominal obesity-metabolic syndrome Diseases 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 235000015107 ale Nutrition 0.000 description 1
- 229940069521 aloe extract Drugs 0.000 description 1
- AFHJQYHRLPMKHU-OSYMLPPYSA-N aloin A Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1[C@@H]1C2=CC(CO)=CC(O)=C2C(=O)C2=C(O)C=CC=C21 AFHJQYHRLPMKHU-OSYMLPPYSA-N 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000006851 antioxidant defense Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 230000010001 cellular homeostasis Effects 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 238000002784 cytotoxicity assay Methods 0.000 description 1
- 231100000263 cytotoxicity test Toxicity 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 208000010643 digestive system disease Diseases 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 230000003090 exacerbative effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013861 fat-free Nutrition 0.000 description 1
- 229960002143 fluorescein Drugs 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 229940074391 gallic acid Drugs 0.000 description 1
- 235000004515 gallic acid Nutrition 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- AFHJQYHRLPMKHU-UHFFFAOYSA-N isobarbaloin Natural products OC1C(O)C(O)C(CO)OC1C1C2=CC(CO)=CC(O)=C2C(=O)C2=C(O)C=CC=C21 AFHJQYHRLPMKHU-UHFFFAOYSA-N 0.000 description 1
- 230000004322 lipid homeostasis Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 231100000849 liver cell damage Toxicity 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000003617 peroxidasic effect Effects 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000012925 reference material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000003656 tris buffered saline Substances 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/886—Aloeaceae (Aloe family), e.g. aloe vera
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/324—Foods, ingredients or supplements having a functional effect on health having an effect on the immune system
Abstract
Description
The present invention relates to an antioxidative composition comprising an extract extracted from aloe vera with a growth period of 4 to 6 months as an active ingredient.
Oxidation is an essential process in the production of energy to supply fuel in the biological processes of living organisms, but free radicals and reactive oxygen species (ROS), which are constantly produced in vivo, cause cell death and tissue damage (Turkoglu, Duru, Mercan, Kivrak, & Gezer, 2007).
Oxidative stress appears to be an imbalance between the production of reactive oxygen species and the antioxidant defense system of cells. Excessive production of reactive oxygen species causes oxidative stress, loss of cell function, ultimately apoptosis or necrosis. Oxidative stress caused by active oxygen species induces chronic diseases, so antioxidative activity or free radicals Inhibition is very important in protecting cells from toxic substances caused by oxidative stress.
The liver plays an important role in maintaining systemic lipid homeostasis and is inherently vulnerable to ROS damage (Aruoma, 1994). Meanwhile, the liver plays an important role in detoxification as well as supplying energy substrates to surrounding tissues through Cori cycle / glycogen catabolism (Hamelet, Demuth, Paul, Delabar, & Janel, 2007). Oxidative stress-related factors may be associated with liver dysfunction, and liver dysfunction is associated with oxidation of nutrients (Yang, Li, Shi, & Le, 2008). In addition, ROS has deleterious effects on liver cells by damaging DNA, lipids and proteins, destroying cellular homeostasis and exacerbating features of metabolic syndrome (Ravel, Lymas, Nitta, Mohuczy, Lemaster, Kim et al., 2006; Kohen, & Nyska, 2002).
Studies on antioxidants have been carried out in 1969 when McCord and Fridovich discovered SOD, an enzyme that clears superoxide radicals. In recent years, diseases such as aging and adult diseases have been associated with active oxygen, , And research on natural antioxidants using plant extracts has attracted much attention because of the potential side effects of synthetic antioxidants.
Aloe is a tropical or subtropical perennial plant belonging to the Liliaceae and is cultivated all over the world, with 300 to 360 species. Aloe vera (Aloe vera) and Alloe avoresence are the cultivars that are mainly grown in Korea. Aloe emodine, barbaloin, , Aloesin, and sterols and terpenoids, and it is known that they have antioxidant activity, antibacterial activity, antitumor activity, bactericidal action, anti-inflammatory action, Have been used for the treatment of burns, skin diseases and digestive system diseases by folk remedies and have been known to be effective for the prevention and improvement of incurable diseases (Doo-Ho Shin, Korean J. Food & Nutr, 2007, 20, 4 : 399-405).
However, studies on such efficacy and components have been conducted mainly on adult aloe leaves grown for 2 to 3 years, and there are few studies on the effect of aloe depending on the growth period. Aloe was different in size and thickness depending on the growth period. The average length of aloe with 3 months of growth was 20 cm and the average of aloe with 6 months was 45 cm. However, there is no study on the bioactivity of aloe according to the growth period.
Korean Patent Laid-Open No. 10-2012-0044583 (method for promoting antioxidative activity of aloe extract using radiation), Korean Patent Laid-Open No. 10-2013-0060954 (method for producing enzyme extract from aloe gel and enzyme extract of aloe gel However, there have been disclosed processes for further processing to enhance the antioxidative activity of aloe. However, technical features for promoting the antioxidative activity of aloe based on the growth period have not been disclosed.
An object of the present invention is to provide an antioxidative composition comprising, as an active ingredient, an extract obtained by selecting Aloe vera, which has the highest antioxidative activity from aloe vera with different antioxidative activities depending on the growth period.
In order to achieve the above object, the present invention provides an antioxidative composition comprising an extract extracted from aloe vera with a growth period of 4 to 6 months as an active ingredient.
The aloe vera extract is characterized by containing polyphenols and flavonoids.
The antioxidant composition is characterized in that the Aloe vera extract is contained in an amount of 40 to 100% by weight.
The aloe vera extract is prepared by (1) removing the crust of aloe vera leaves having a growth period of 4 to 6 months and pulverizing the same; (2) extracting Aloe vera extract by adding water to aloe vera crushed in step (1); (3) centrifuging the aloe vera extract extracted in step (2) for 10 to 20 minutes to obtain a supernatant; And (4) lyophilizing the supernatant obtained in the step (3).
The aloe vera pulverized in the step (2) and water are mixed with each other at a ratio of 1:20 to 1:30 (g / mL).
The temperature and the time to be extracted in the step (2) are 15 to 25 ° C and 20 to 30 hours.
The antioxidant composition is further characterized by being used as a health functional food for preventing and improving liver diseases including hepatitis and liver cirrhosis, further comprising a pharmaceutically acceptable extract, a nutritional component or a food supplementary additive.
The antioxidant composition further comprises a pharmaceutically acceptable carrier or excipient and is characterized by being used as a medicine for preventing and treating liver diseases including hepatitis and liver cirrhosis.
According to the present invention, by using the extract extracted from aloe vera with a growth period of 4 to 6 months as a natural antioxidant, the antioxidative activity is remarkably improved as compared with the extract extracted from the adult aloe vera, Preventing damage to cells and preventing and treating liver diseases including hepatitis and liver cirrhosis.
1 is a cytotoxicity measurement of Aloe vera extract according to the present invention.
FIG. 2 is a graph showing the cytoprotective effect of Aloe vera extract according to the present invention.
3 is a graph showing the ROS inhibitory activity of Aloe vera extract according to the present invention.
FIG. 4 is a graph showing the ability of the extract of Aloe vera according to the present invention to inhibit the death of normal liver cells due to oxidative stress.
FIG. 5 shows the effect of the aloe vera extract according to the present invention on the expression of Bcl-2, Bax and β-actin proteins.
Hereinafter, the present invention will be described in detail.
The present invention provides an antioxidant composition comprising an extract extracted from aloe vera with a growth period of 4 to 6 months as an active ingredient.
The aloe vera extract preferably contains a large amount of polyphenols and flavonoids.
The antioxidant composition preferably contains 40 to 100% by weight, preferably 50 to 70% by weight, of the aloe vera extract.
The aloe vera extract is prepared by (1) removing the crust of aloe vera leaves having a growth period of 4 to 6 months and pulverizing the same; (2) extracting Aloe vera extract by adding water to aloe vera crushed in step (1); (3) centrifuging the aloe vera extract extracted in step (2) for 10 to 20 minutes to obtain a supernatant; And (4) lyophilizing the supernatant obtained in the step (3).
In step (2), the pulverized aloe vera and water are preferably mixed and mixed at a ratio of 1:20 to 1:30 (g / mL). The extract of Aloe vera according to the present invention can be obtained by extracting water with low toxicity by using water as a solvent and directly ingesting it.
The temperature and the time for extraction in the step (2) are preferably 15 to 25 ° C and 20 to 30 hours.
The antioxidant composition may further comprise a food-acceptable extract, a nutritional component or a food-aid additive and may be used as a health functional food for preventing and improving liver diseases including hepatitis and liver cirrhosis. 0.1 to 30 parts by weight of an antioxidant composition is preferably contained.
The antioxidant composition may further comprise a pharmaceutically acceptable carrier or excipient and may be used as a medicament for preventing and treating liver diseases including hepatitis and liver cirrhosis. The antioxidant composition may contain 0.01 to 30 parts by weight of an antioxidative composition per 100 parts by weight of the medicament .
Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not construed as being limited by these embodiments.
Examples. Production of Aloe Vera Extract
Aloe vera used Aloe barbadensis Miller ( Aloe vera Linne ).
Aloe vera and aloe samples were prepared by crushing the leaves with the bark of aloe vera and adult for 2 months, 4 months, 6 months and 8 months.
1 g of each aloe sample was mixed with 20 ml of tertiary distilled water, and the mixture was stirred at room temperature for 24 hours for extraction. The mixture was centrifuged at 3,000 rpm for 20 minutes, and the supernatant was lyophilized at -70 ° C for 3 days to obtain aloe vera The extract was prepared.
In addition, 8 mg of each of the aloe vera extracts prepared above was dissolved in 1 mL of distilled water and diluted several times to prepare aloe vera extract solutions having various concentrations.
Experimental Example 1. DPPH, hydroxyl, alkyl, and superoxide radical scavenging ability
1. DPPH radical scavenging ability
1,1-diphenyl-2-picryl hydrazyl (DPPH) exhibits a deep purple color and is itself a radical of the nitrogen center, which is stabilized by the delocalization of radical electrons Lt; / RTI > The measurement method of DPPH radical scavenging ability using ESR is as follows.
30 μl of 60 μM DPPH dissolved in methanol was mixed with 30 μl of aloe vera extract solution by concentration. After vortexing vigorously for 10 seconds, the mixture was allowed to stand at room temperature for 2 minutes, transferred to a capillary tube and analyzed by ESR spectrometer , JEOL Ltd., Tokyo, Japan). The ESR spectrometer conditions were central field, 3475 G; modulation frequency, 100 kHz; modulation amplitude, 2 G; microwave power, 5 mW; gain, 6.3 × 10 5, and temperature, 298 K.
The results are shown in Table 1. The DPPH radical scavenging activity of aloe vera samples by the growth period was highest (IC 50 = 0.26 mg / ml) in 6-month samples.
2. Hydroxyl radical scavenging ability
Hydroxyl radicals were generated based on the Fenton reaction in which iron (Fe) ion reacted with hydrogen peroxide to measure the amount of DMPO (5,5-dimethyl-1-pyrolin N-oxide) Were measured.
20 μl of 0.3 M DMPO, 20 μl of 10
The results are shown in Table 1. The hydroxyl radical scavenging activity of aloe vera samples at the growth period was about 31% at the concentration of 2.0 mg / ml for 4 months.
3. Alkyl radical scavenging ability
The alkyl radical is produced by AAPH (2,2'-azobis (2-amidinopropane) dihydride) to give POBN (a- (4-propynyl 1-oxide) And the amount of the reaction was measured.
20 [mu] l of phosphate buffered saline (PBS), 20 [mu] l of various concentrations of aloe vera extract solution, 20 [mu] l of 40 mM AAPH and 20 [mu] l of 40 mM POBN were added successively and vortexed vigorously for 10 seconds. The mixture was reacted in a 37 ° C water bath for 30 minutes, transferred to a capillary, and the amount of alkyl radicals generated was measured by an ESR spectrometer. The ESR spectrometer conditions at this time were the central field, 3475 G; modulation frequency, 100 kHz; modulation amplitude, 2 G; microwave power, 10 mW; gain, 6.3 × 10 5, and temperature, 298 K.
The results are shown in Table 1. The alkyl radical scavenging activity of aloe vera samples by the growth period was the best in 6 month samples (IC 50 = 0.499 mg / ml).
4. Superoxide radical scavenging ability
Superoxide radicals are produced by exposure to ultraviolet light on riboflavin / EDTA solutions. The method of measuring superoxide radical scavenging ability using ESR is as follows.
20 μl of 0.8 mM riboflamin, 20 μl of 0.8 M DMPO, 20 μl of 1.6 mM ethylenediamine tetraacetic acid (EDTA) and 20 μl of various concentrations of aloe vera extract solution (dissolved in ultrapure distilled water) , Vortexed vigorously for 10 seconds, exposed to ultraviolet light of 365 nm for 1 minute, transferred to a capillary, and measured by ESR spectrometer. The ESR spectrometer conditions at this time were the central field, 3475 G; modulation frequency, 100 kHz; modulation amplitude, 2 G; microwave power, 10 mW; gain, 6.3 × 10 5, and temperature, 298 K.
The results are shown in Table 1. The superoxide radical scavenging activity of aloe vera samples at the growth period was most excellent when the 6-month sample had a scavenging activity of about 20% at a concentration of 2.0 mg / ml.
Experimental Example 2. Measurement of ABTS, FRAP, ORAC, polyphenol and flavonoid
1. ABTS
The antioxidant activity of ABTS radicals was measured by the removal of ABTS free radicals produced by the reaction with potassium persulfate by antioxidants in the extract and discoloration of the radical - specific cyan color.
After dissolving potassium persulfate in 7 mM ABTS solution to 2.4 mM, it was reacted in the dark room for 12-16 hours. After adjusting with distilled water to an absorbance of 1.5 at 414 nm, 3.0 ml of aloe vera extract solution was added, and 1.0 ml of aloe vera extract solution was added and reacted at room temperature for 10 minutes to measure absorbance at 414 nm.
The results are shown in Table 2. The 6 - month samples showed the highest ABTS radical inhibitory activity in the aloe vera samples.
2. FRAP
The FRAP reagent was prepared by mixing 300 mM acetate buffer (pH 3.6), 10 mM TPTZ dissolved in 40 mM HCl and 20 mM FeCl 3 .6H 2 O at a ratio of 10: 1: 1 (v / v / v) . Next, 0.15 ml of the aloe vera extract solution diluted to a solids content of 1 to 5 mg and 3.0 ml of the FRAP reagent were mixed, reacted at 37 ° C for 5 minutes, and the absorbance was measured at 593 nm. Results in a 4 · 7H 2 O to a standard FeSO substance were expressed as mM FeSO 4 eq./mg extract.
The results are shown in Table 2. The 6 - month samples showed the highest reducing power among the aloe vera samples.
3. ORAC
The antioxidative activity of Aloe vera extracts was measured by the rate of decrease of fluorescent light by the production and disappearance of peroxy radical.
2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH) was used for the generation of peroxidation radicals and 20 uM Trolox was used as the standard for comparison of antioxidant activity. Fluorescent standard solutions were prepared by adding fluorescein sodium salt (Sigma-Aldrich Co.) to 75 mM phosphate buffer at a concentration of 78 nM.
To determine antioxidant activity, 50 μl of fluorescent standard solution was added to 50 μl of aloe vera extract and standard solution. AAPH, a peroxidative radical inducer, was added to phosphate buffer to dilute to 221 mM, and then 25 μl of the diluted solution was reacted with a spectrofluorometer (SpectraMax M2 / M2e, CA, USA), fluorescence was measured every 5 minutes for 2 hours at excitation 485 nm and emission 538 nm. ORAC was calculated using trolox, which is as follows. The AUC was calculated as the net area minus the curve area obtained by adding AAPH to the fluorescein in the curve area obtained by the sample.
The results are shown in Table 2. All samples showed similar activity.
4. Polyphenol
The total polyphenol content was determined by adding 1.0 ml of 1.0 N Folin-Ciocalteau reagent and 1.0 ml of 20% Na 2 CO 3 solution to 1.0 ml of the aloe vera extract solution, and allowing to stand at room temperature for 30 minutes. The resultant was analyzed with a spectrophotometer (SECOMAM, Ales, France ) Was used to measure the absorbance at 700 nm.
The total phenol content of the sample extracts was calculated from the standard calibration curve obtained by analyzing gallic acid (Sigma Co., St. Louis, Mo., USA) at a concentration of 0 to 200 μg / equivalents (mg GAE / g extract).
The results are shown in Table 2. The 6 - month samples showed the highest contents in the Aloe Vera samples.
5. Flavonoid
Total flavonoid content was determined by adding 0.1 ml of 10% aluminum nitrate, 0.1 ml of 1.0 M potassium acetate, and 4.3 ml of ethanol to 0.5 ml of aloe vera extract solution. The mixture was allowed to stand at room temperature for 40 minutes and then absorbed at 415 nm.
The total flavonoid content of the extract was calculated from a standard calibration curve obtained at a concentration range of 0 to 1 mg / ml using Catechin (Sigma Co.) as a reference material.
The results are shown in Table 2. The 6 - month samples showed the highest contents in the Aloe Vera samples.
Then, the following experiment was conducted using a 6-month sample having the best antioxidant activity.
Experimental Example 3. Cytotoxicity of Aloe Vera Extract
Cell viability was measured by 3- (4,5-dimethylthiazole-2-yl) -2,5-diphenyl-tetrazolium bromide (MTT) reduction method.
Chang cells were seeded on 24-well plates at a density of 7 × 10 4 cells / ml in 1.0 ml each, and cultured for 24 hours. Each sample was added to cells for final concentrations of 0, 0.25, 0.5, 1.0 and 2.0 mg / ml And cultured for 24 hours. After that, MTT solution (5.0 mg / ml) was added and cultured at 37 ° C for additional 4 hours. MTT was reduced and the medium was carefully removed so that the generated formazan did not fall off the medium. 200 μl of DMSO was added and mixed for 20 minutes. Absorbance was measured at 540 nm.
The results are shown in Fig. Cytotoxicity assay of Aloe vera extract for 6 months showed no toxicity to normal liver cells at all concentrations.
EXPERIMENTAL EXAMPLE 4 Cell Protection Effect of Aloe Vera Extract
Cell viability was measured by 3- (4,5-dimethylthiazole-2-yl) -2,5-diphenyl-tetrazolium bromide (MTT) reduction method.
Chang cells were plated on 24-well plates at a concentration of 7 × 10 4 cells / ml in 1.0 ml each and cultured for 24 hours. Each sample was treated with cells to final concentrations of 0, 0.25, 0.5, 1.0 and 2.0 mg / ml, and after 1 hour, 80 μM t- BHP was added and incubated for 24 hours. After that, MTT solution (5.0 mg / ml) was added and cultured at 37 ° C for additional 4 hours. MTT was reduced and the medium was carefully removed so that the generated formazan did not fall off the medium. 200 μl of DMSO was added and mixed for 20 minutes. Absorbance was measured at 540 nm.
The results are shown in Fig. The 6 - month - old Aloe vera extract showed a concentration - dependent cytoprotective effect in normal liver cells.
Experimental Example 5: ROS inhibition of aloe vera extract
The degree of reactive oxygen species generated in the cells was measured by fluorescence spectrophotometer using 2 ', 7'-dichlorofluorescein diacetate (DCF-DA) which reacts with reactive oxygen species and emits fluorescence.
2 × 10 4 Chang cells were plated on a 96-well black plate and cultured in a 5% CO 2 incubator at 37 ° C. for 24 hours. Then, the samples were pretreated for 1 hour. Then, 80 μM t- BHP was treated for 30 minutes, and DCF-DA was added to a final concentration of 10 μM and incubated at 37 ° C. for 30 minutes. After washing three times with PBS, the fluorescence was measured by excitation (485 nm; emission, 538 nm) using a fluorescence spectrometer (SpectraMax M2 / M2e, CA, USA).
The results are shown in Fig. Six months old Aloe vera extract showed concentration dependent ROS inhibitory activity in normal liver cells.
Experimental Example 6. Inhibition of normal liver cell death due to oxidative stress of aloe vera extract
We investigated whether aloe vera extract could inhibit normal liver cell damage due to oxidative stress. Propidium iodide (PI) is a fluorescent dye frequently used to measure apoptosis, an automatic apoptosis cell. Since it binds to DNA, apoptosis and cell cycle can be deduced from the amount of PI-stained DNA.
2.1 × 10 5 hepatocytes were inoculated into a 6-well plate, treated with 80 μM t- BHP or 80 μM t- BHP + aloe vera extract for 24 h, and incubated in a CO 2 incubator for 24 h . After completion of the reaction, the cells were harvested and centrifuged at 13,000 rpm for 3 minutes at 4 ° C. Discard the supernatant, add 300 μl of phosphate buffer, mix well, add 1 ml of cold ethanol containing 0.5% of tween-20, Respectively. After 24 hours, the mixture was centrifuged at 13,000 rpm for 3 minutes at 4 ° C. The supernatant was discarded, and 1 ml of phosphate buffer was added thereto. The mixture was mixed well and centrifuged at 13,000 rpm for 3 minutes. To this, 300 μl of iodopropidium solution (
The results are shown in Fig. t-BHP alone, and that 6-month-old Aloe vera extract decreased the normal liver cell death in a dose-dependent manner.
Experimental Example 7 Effect of Aloe Vera Extract on Bcl-2, Bax, and β-actin Protein Expression
Chang cells, a normal liver cell, were divided into 6-well plates at a concentration of 2 × 10 5 cells / ml and cultured for 24 hours. Then, 6-month aloe vera extracts at 0.25, 0.5 and 1.0 mg / 80 μM t- BHP and cultured for 24 hours. The cells were washed with phosphate buffered saline (PBS), harvested and centrifuged. The lysis buffer was added to the pellet, incubated on ice, and centrifuged at 13,000 rpm for 15 minutes. The collected supernatant was quantified by Bradford method using bovine serum albumin (BSA) as a standard. 20 μg of protein was inactivated in 5 × sample buffer at 100 ° C. for 5 minutes and electrophoresed on 12% SDS polyacrylamide gel. The separated proteins were transferred to PVDF membrane and blocked for 90 minutes in 5% non-fat dry milk. Bcl-2, Bax and actin were incubated overnight at 4 ° C in a ratio of 1: 1,000 and 1: 2,000, and then washed three times for 10 minutes with tris-buffered saline Tween-20 (TBST) Was diluted at a ratio of 1: 1,000 and adhered at room temperature for 2 hours. Western blotting was performed with LAS-3000 (Fujifilm, Tokyo, Japan) by treating ECL (Amersham Pharmacia Biotech, Piscataway, NJ, USA) with TBST buffer for 10 minutes three times.
The results are shown in Fig. Oxidative stress + 6 month Aloe vera extract treated group showed higher expression level in BCl-2 than oxidative stress-treated group, and similar amount in Bax.
Having described specific portions of the present invention in detail, it will be apparent to those skilled in the art that this specific description is only a preferred embodiment and that the scope of the present invention is not limited thereby. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.
Claims (8)
Wherein the aloe vera extract contains polyphenols and flavonoids.
Wherein the antioxidant composition comprises 40 to 100% by weight of an aloe vera extract.
The aloe vera extract is prepared by (1) removing the crust of aloe vera leaves having a growth period of 4 to 6 months and pulverizing the same; (2) extracting Aloe vera extract by adding water to aloe vera crushed in step (1); (3) centrifuging the aloe vera extract extracted in step (2) for 10 to 20 minutes to obtain a supernatant; And (4) lyophilizing the supernatant obtained in the step (3).
Wherein the aloe vera pulverized in the step (2) and water are mixed and extracted at a ratio of 1:20 to 1:30 (g / mL).
Wherein the temperature and the time of extraction in the step (2) are 15 to 25 DEG C and 20 to 30 hours.
The antioxidant composition according to claim 1, wherein the antioxidant composition further comprises a food-acceptable extract, a nutritional component or a food-aid additive, and is used as a health functional food for preventing and improving liver diseases including hepatitis and liver cirrhosis.
Wherein the antioxidant composition further comprises a pharmaceutically acceptable carrier or excipient and is used as a medicament for preventing and treating liver diseases including hepatitis and liver cirrhosis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20130070759A KR20140147949A (en) | 2013-06-20 | 2013-06-20 | Antioxidant composition comprising aloe vera extracts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20130070759A KR20140147949A (en) | 2013-06-20 | 2013-06-20 | Antioxidant composition comprising aloe vera extracts |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR20150051035A Division KR20150046777A (en) | 2015-04-10 | 2015-04-10 | Antioxidant composition comprising aloe vera extracts |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20140147949A true KR20140147949A (en) | 2014-12-31 |
Family
ID=52676473
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR20130070759A KR20140147949A (en) | 2013-06-20 | 2013-06-20 | Antioxidant composition comprising aloe vera extracts |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR20140147949A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180119189A (en) * | 2017-04-24 | 2018-11-02 | 주식회사 코씨드바이오팜 | Skin moisture and anti-aging cosmetic composition with the extract of Aloe Vera by Chojeong mineral water |
-
2013
- 2013-06-20 KR KR20130070759A patent/KR20140147949A/en active Application Filing
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180119189A (en) * | 2017-04-24 | 2018-11-02 | 주식회사 코씨드바이오팜 | Skin moisture and anti-aging cosmetic composition with the extract of Aloe Vera by Chojeong mineral water |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ebrahimzadeh et al. | Antioxidant and free radical scavenging activity of H. officinalis L. var. angustifolius, V. odorata, B. hyrcana and C. speciosum | |
| Carvalho et al. | Human cancer cell antiproliferative and antioxidant activities of Juglans regia L. | |
| Cui et al. | Protective effects of polyphenols-enriched extract from Huangshan Maofeng green tea against CCl4-induced liver injury in mice | |
| Bittencourt et al. | The protective effects of guaraná extract (Paullinia cupana) on fibroblast NIH-3T3 cells exposed to sodium nitroprusside | |
| Farhan et al. | Preliminary phytochemical screening and extraction of polyphenol from stems and leaves of a Lebanese plant Malva parviflora L | |
| Heo et al. | Antioxidant activities of red algae from Jeju Island | |
| Chiu et al. | The antioxidant and cytoprotective activity of Ocimum gratissimum extracts against hydrogen peroxide-induced toxicity in human HepG2 cells | |
| Fahmy et al. | Antihepatotoxic efficacy of Mangifera indica L. polysaccharides against cyclophosphamide in rats | |
| Bhatt et al. | Camellia sinensis L: the medicinal beverage: a review | |
| SHAFAY et al. | Antioxidant, antidiabetic, anti-inflammatory and anticancer potential of some seaweed extracts | |
| Jin et al. | In vitro and in vivo antioxidant properties of water and methanol extracts of linden bee pollen | |
| Jadhav et al. | Free radical scavenging and antioxidant activity of Punica granatum Linn | |
| Jing et al. | Antioxidant activity, antitumor effect, and antiaging property of proanthocyanidins extracted from Kunlun Chrysanthemum flowers | |
| Tan et al. | Protective effects of papaya extracts on tert-butyl hydroperoxide mediated oxidative injury to human liver cells (An in-vitro study) | |
| Bhaskara Rao et al. | Phytochemical composition and antioxidant activity of Ficus benghalensis (Moraceae) leaf extract | |
| Lee et al. | Antioxidant properties of tidal pool microalgae, Halochlorococcum porphyrae and Oltamannsiellopsis unicellularis from Jeju Island, Korea | |
| Teoh et al. | Evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of Gynura procumbens (Compositae) | |
| KR20140135488A (en) | Antioxidant composition comprising cockscome flower extracts | |
| Zhao et al. | Antioxidant activities of crude phlorotannins from Sargassum hemiphyllum | |
| Kiran et al. | In vitro evaluation of antioxidant potentiality of seeds of Psoralea corylifolia L | |
| KR20150046777A (en) | Antioxidant composition comprising aloe vera extracts | |
| KR20140147949A (en) | Antioxidant composition comprising aloe vera extracts | |
| KR20160141463A (en) | Anti-oxidation and anti-inflammatory composition comprising the extract of aralia continentalis | |
| Bell et al. | In vivo antioxidant activity of bark extract of Bixa orellana L. against acetaminophen–induced oxidative stress | |
| Wang et al. | Pinus massoniana bark extract protects against oxidative damage in L-02 hepatic cells and mice |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| E902 | Notification of reason for refusal | ||
| N231 | Notification of change of applicant | ||
| E601 | Decision to refuse application | ||
| A107 | Divisional application of patent |