JP2006176425A - Collagen production promoter and skin care preparation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a collagen production promoter or skin care preparation containing, as active ingredient, a substance having collagen production-promoting activity that is found from highly safe natural matter. <P>SOLUTION: The collagen production promoter or skin care preparation contains, as the active ingredient, extract from the fruit part of Prunus mume Siebold et Zuccarini. The extract is obtained, preferably by the following process: fruit juice or a concentrate thereof is mixed with an extracting solvent and the resulting mixed liquid is left to stand at a dark cold place for 5-10 days, wherein the extracting solvent is preferably an organic solvent or its mixture with water. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a collagen production promoter and a skin external preparation.

  The epidermis and dermis of the skin are composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin, collagen, and hyaluronic acid that are outside these cells and support the skin structure. In young skin, the interaction between these skin tissues is kept constant, so that moisture retention, flexibility, elasticity, etc. are secured, and the skin is maintained in a fresh state with its tension and gloss. .

  However, when there is an influence of certain external factors such as irradiation of ultraviolet rays, significant drying of air, excessive skin washing, etc., or when aging progresses, the production amount of collagen, which is a main component of the extracellular matrix, is increased. In addition to a decrease, it causes a decrease in elasticity due to crosslinking. As a result, the skin's moisturizing function and elasticity are reduced, and the keratin begins to exfoliate abnormally, so that the skin loses tension and gloss, and exhibits aging symptoms such as rough skin and wrinkle formation.

As described above, changes associated with skin aging, that is, wrinkles, dullness, disappearance of texture, decrease in elasticity, and the like, are associated with reduction and degeneration of dermal matrix components such as collagen. Therefore, it is considered that collagen production in dermal fibroblasts can be promoted to prevent and / or improve skin aging. 1), a lotus germ extract (see Patent Document 2), a pentagon extract (see Patent Document 3), and the like.
JP 2000-191498 A JP 2002-29980 A JP 2002-226323 A

  An object of the present invention is to find a highly safe natural product having a collagen production promoting action, and to provide a collagen production promoter or an external preparation for skin containing the same as an active ingredient.

  In order to solve the above-mentioned problems, the collagen production promoter of the present invention contains an extract from a ume fruit part as an active ingredient, and the skin external preparation of the present invention is extracted from a ume fruit part. It is characterized by blending. The extract is preferably obtained by leaving a mixture obtained by mixing a fruit juice obtained from a fruit portion of ume or a concentrate thereof and an extraction solvent in a cool dark place for 5 to 10 days. Furthermore, the extraction solvent is preferably an organic solvent or a mixed solution of an organic solvent and water.

  ADVANTAGE OF THE INVENTION According to this invention, the collagen production promoter or skin external preparation which can accelerate | stimulate the production of collagen in a dermal layer fibroblast and can prevent and / or improve skin aging can be provided.

The present invention will be described below.
[Collagen production promoter]
The collagen production promoter of the present invention contains an extract from the fruit part of ume as an active ingredient.

  Here, in the present invention, the “extract” refers to an extract obtained from ume as an extraction raw material, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or a crude product thereof. Both products and purified products are included.

The extraction raw material used in the present invention is ume (scientific name: Prunus mume Siebold et Zuccarini).
Plum (Prunus mume Siebold et Zuccarini) is a deciduous small tree belonging to the family Rosaceae and is planted for ornamental and edible purposes. It is native to central China, and has come to Japan since ancient times and has become wild in Kyushu. The part which can be used as an extraction raw material is a fruit part.

  Although details of the substance having a collagen production promoting action contained in the extract from the ume fruit part are unknown, an extract having a collagen production promoting action can be obtained from the fruit part of the ume by the method described later.

  After washing the fruit portion of the ume, it is crushed as it is or using a crusher, the pulp and seeds are separated, and the ume juice obtained by squeezing the pulp or the ume juice is concentrated under reduced pressure. By subjecting the obtained concentrated solution to an extraction treatment with an extraction solvent, an extract having a collagen production promoting action can be obtained. The fruit portion of the ume may be used as an extraction raw material after pretreatment such as degreasing with a nonpolar solvent such as hexane. By performing pretreatment such as degreasing, extraction with a polar solvent can be performed efficiently.

  As the extraction solvent, an organic solvent or a mixed liquid of an organic solvent and water is preferably used, and a hydrophilic organic solvent is preferably used as the organic solvent. In particular, it is preferable to use a hydrophilic organic solvent alone or in admixture of two or more, or to use a mixed liquid of a hydrophilic organic solvent and water.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, and those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of hydrophilic organic solvents that can be used as the extraction solvent include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; -C2-C5 polyhydric alcohols, such as butylene glycol, propylene glycol, and glycerol, etc. are mentioned.

  When using the liquid mixture of 2 or more types of hydrophilic organic solvents or the liquid mixture of a hydrophilic organic solvent and water as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when using a liquid mixture of water and a lower aliphatic alcohol, it is preferable to mix 1 to 90 parts by weight of a lower aliphatic alcohol with respect to 10 parts by weight of water. When using a mixed solution, it is preferable to mix 1 to 40 parts by mass of a lower aliphatic ketone with 10 parts by mass of water, and when using a mixed solution of water and a polyhydric alcohol, water 10 It is preferable to mix 10 to 90 parts by mass of polyhydric alcohol with respect to parts by mass.

  The extraction process can be performed as follows. For example, a ume juice or a mixture of concentrated ume juice and an extraction solvent is allowed to stand in a cool and dark place for a predetermined period to elute soluble components into the extraction solvent, and then filtered to remove sediment and precipitates. By removing it, an extract can be obtained. It is preferable that the temperature conditions of the said extraction process are 0-15 degreeC, and it is especially preferable that it is 5-10 degreeC. The illuminance condition for the extraction process is preferably 0 to 0.1 lx, particularly preferably 0 to 0.01 lx. Further, the period of leaving the ume juice or the concentrated solution of ume juice and the extraction solvent in a cool and dark place is preferably 5 to 10 days, and particularly preferably 6 to 8 days. By performing the extraction process within the above range, it is possible to improve the extraction efficiency of soluble components from the ume fruit part. The obtained extract is diluted, concentrated, dried, purified, etc. according to a conventional method in order to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crude purified product or a purified product thereof. Processing may be performed.

  Purification can be performed by, for example, activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like. The obtained extract can be used as it is as an active ingredient of a collagen production promoter, but its concentrated activity or dried product is easier to use since its physiological activity may be low.

  Extracts from the ume fruit part can be purified for the purpose of decoloring, deodorizing, etc. within a range that does not cause a decrease in their physiological activity, but they are used in large quantities when blended into skin external preparations. Since it is not a thing, there is no practical problem even if it is not purified.

  Since the extract from the ume fruit part obtained as described above has a collagen production promoting action, it can be used as an active ingredient of a collagen production promoting agent utilizing these actions.

  The collagen production promoter of the present invention may consist only of an extract from the ume fruit part, or may be a formulation of the above extract.

  Extract from ume fruit part is any powder, granule, tablet, liquid, etc., using pharmaceutically acceptable carriers such as dextrin, cyclodextrin, etc. It can be formulated into a form. The extract from the ume fruit part can be used by blending with other compositions (for example, a skin external preparation described later), and can also be used as an ointment, an external liquid, a patch, and the like. In this case, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring agent and the like can be used.

  In addition, the collagen production promoter of this invention can mix | blend the other natural extract which has a collagen production promotion effect | action as needed, and can use it as an active ingredient.

  The collagen production promoter of the present invention can promote the production of collagen in dermal fibroblasts through the collagen production promoting action of the extract from the fruit portion of ume. Thereby, the aging of the skin accompanying the decrease in the production amount of collagen can be prevented and / or improved. However, the collagen production promoter of the present invention can be used for all uses other than these uses that are meaningful for exerting a collagen production promoting action.

[Skin external preparation]
An extract from the fruit part of ume has a collagen production promoting action, can prevent and / or improve skin aging, and has excellent usability and safety when applied to the skin. Therefore, it is suitable for blending into an external preparation for skin. In this case, the external preparation for skin may be blended with an extract from the fruit part of ume or a collagen production promoter formulated from an extract from the fruit part of ume. A collagen production promoting action can be imparted to the external preparation for skin by blending an extract from the fruit part of ume or the above-mentioned collagen production promoter with the external preparation for skin.

  Here, “skin external preparation” means various drugs applied to the skin, and includes, for example, cosmetics, quasi drugs, pharmaceuticals, and the like.

  There are no particular limitations on the type of external preparation for skin that can be blended with an extract from the fruit part of ume. Examples include ointments, creams, emulsions, lotions, packs, jellies, bath preparations, lip balms, and lipsticks. Can be mentioned.

  When the extract from the fruit part of ume is blended into the external preparation for skin, the blending amount can be appropriately adjusted in consideration of the type of external skin preparation, the purpose of use, the sex of the user, the symptoms of the user, etc. A suitable blending ratio is about 0.005 to 10% by mass in terms of a standard extract.

  The skin external preparation of the present invention is a main agent, auxiliary agent or other component used for the production of a normal skin external preparation, for example, an astringent, as long as it does not interfere with the collagen production promoting action of the extract from the fruit portion of ume , Disinfectant / antibacterial agent, whitening agent, UV absorber, moisturizer, cell activator, anti-inflammatory / antiallergic agent, antioxidant / active oxygen remover, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, Esters, surfactants, fragrances and the like can be used in combination. By using together, it becomes a more general product, and the synergistic action between the above-mentioned components used in combination may bring about a superior use effect than would normally be expected.

  The collagen production promoter or skin external preparation of the present invention is preferably applied to humans, but may be applied to animals other than humans as long as the respective effects are exhibited. it can.

  Hereinafter, although a manufacture example, a test example, and a compounding example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to each following example at all.

[Production Example 1]
After washing, crushing, and enucleating 1000 g of ume fruit, juice was obtained to obtain ume fruit juice. The obtained ume fruit juice was concentrated under reduced pressure at 40 ° C., an extraction solvent was added, and the mixture was stirred and then allowed to age for 7 days in a cool dark place. The obtained filtrate was concentrated under reduced pressure at 40 ° C., and the obtained concentrate was dried with a vacuum dryer to obtain a plum extract. 70% by mass of ethanol (mass ratio of water to ethanol = 3: 7), 50% by mass of ethanol (mass ratio of water to ethanol = 1: 1), or each extract when using water as the extraction solvent The yield is shown in Table 1.

[Table 1]
Sample extraction solvent extract yield (% by mass)
1 70% by weight ethanol 1.8
2 50% ethanol 2.1
3 Water 2.8

[Test Example 1] Collagen production promoting action test The extracts from the fruit parts of ume obtained in Production Example 1 (Samples 1 to 3) were tested for collagen production promoting action.

Human fibroblasts were seeded in a 96-well plate and cultured for several days in a sample-added medium (sample concentrations: 100 μg / mL and 25 μg / mL) at 37 ° C. and 5% CO ₂ -95% air. Thereafter, 90 μL of the supernatant was transferred to an ELISA plate and adsorbed on the plate overnight at a temperature of 4 ° C., then the solution was removed, and a phosphate physiological buffer solution (PBS containing 0.05% Tween-20) was added. -T). Thereafter, a blocking operation was performed with a physiological phosphate buffer containing 1% bovine serum albumin. The culture solution was removed, washed with PBS-T, and reacted with an anti-human collagen type I antibody (rabbit IgG, manufactured by Chemicon). The culture solution was removed, washed with PBS-T, reacted with HRP-labeled anti-rabbit IgG antibody, and then the same washing operation was performed to perform a color reaction.

The collagen production acceleration rate was calculated by conducting the above ELISA using a standard product, creating a calibration curve, and setting the collagen production amount when no sample was added as 100%.
Table 2 shows the collagen production promotion rate (%) of each sample. In addition, the sample concentration of the sample 2 is a solid content conversion concentration.

[Table 2]
Collagen production promotion rate (%)
Specimen sample concentration: 100 [mu] g / mL Sample concentration: 25 [mu] g / mL
1 487.0 256.7
2 322.2 198.4
3 102.5 98.9

  As shown in Table 2, it was confirmed that the extracts (samples 1 to 3) from the ume fruit part have a collagen production promoting action, and in particular, the ume fruit part obtained using an ethanol aqueous solution as an extraction solvent. It was confirmed that the extract (sample 1 and sample 2) from has excellent collagen production promoting action. In addition, the collagen production promoting action of the extract from the fruit part of ume changes depending on the extract concentration, and it was confirmed that the strength of the collagen production promoting action can be adjusted by adjusting the extract concentration. .

[Formulation Example 1]
An emulsion having the following composition was produced by a conventional method.
Plum fruit part 70 mass% ethanol extract (Production Example 1) 0.1 g
Jojoba oil 4g
2g olive oil
Squalane 2g
Cetanol 2g
2g glyceryl monostearate
Polyoxyethylene cetyl ether (20E.O.) 2.5g
Oleic acid polyoxyethylene sorbitan (20E.O.) 2g
1,3-butylene glycol 3g
Methyl paraoxybenzoate 0.15g
Twilight extract 0.1g
Ginkgo biloba extract 0.1g
Conchiolin 0.1g
Oat extract 0.1g
Chamomile extract 0.1g
0.1g dipotassium glycyrrhizinate
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

[Formulation Example 2]
A lotion having the following composition was produced by a conventional method.
Plum fruit part 70 mass% ethanol extract (Production Example 1) 2g
Glycerin 3g
1,3-butylene glycol 3g
Oleic acid polyoxyethylene sorbitan (20E.O.) 0.5g
Methyl paraoxybenzoate 0.15g
Citric acid 0.1g
Sodium citrate 0.1g
Oil soluble licorice extract 0.1g
Seaweed extract 0.1g
Xylobiose Mixture 0.5g
Kujin extract 0.1g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

[Composition Example 3]
A cream having the following composition was produced by a conventional method.
Plum fruit part 50 mass% ethanol extract (Production Example 2) 0.1 g
Liquid paraffin 5g
Salami beeswax 4g
Setanol 3g
Squalane 10g
Lanolin 2g
Stearic acid 1g
Oleic acid polyoxyethylene sorbitan (20E.O.) 1.5g
3g glyceryl monostearate
1,3-butylene glycol 6g
1.5 g of methyl paraoxybenzoate
Yeast extract 0.1g
Perilla extract 0.1g
Licorice leaf extract 0.1g
Jiuyu Extract 0.1g
Fragrance 0.1g
Purified water remainder (total amount is 100 g)

[Formulation Example 4]
A pack having the following composition was produced by a conventional method.
Plum fruit part 50 mass% ethanol extract (Production Example 1) 5g
Polyvinyl alcohol 15g
Polyethylene glycol 3g
7g of propylene glycol
Ethanol 10g
0.05 g ethyl paraoxybenzoate
Tocopherol acetate 0.1g
Sage extract 0.1g
Toki extract 0.1g
Carrot extract 0.1g
Star fruit leaf extract 0.1g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

The collagen production promoter or external preparation for skin of the present invention can greatly contribute to prevention and / or improvement of skin aging accompanying a decrease in the amount of collagen produced.

Claims (4)

  A collagen production promoter characterized by containing an extract from the fruit part of ume as an active ingredient.   The said extract is obtained by leaving the liquid mixture which mixed the fruit juice obtained from the fruit part of the plum or the concentrate, and the extraction solvent to stand in a cool dark place for 5 to 10 days. Collagen production promoter.   3. The collagen production promoter according to claim 2, wherein the extraction solvent is an organic solvent or a mixed solution of an organic solvent and water. An external preparation for skin characterized by containing an extract from the fruit part of ume.