JPH1095704A - Composition suitable for external use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a composition suitable for external use such as cosmetics and medicines for external use, capable of sufficiently exhibiting actions which a medicine essentially has by combining an extract of the root bark or the trunk bark of Acanthopanax gracilistylus with a specific medicine. SOLUTION: This medicine comprises an extract of the root bark or the trunk bark of Acanthopanax gracilistylus and a medicine selected from a tyrosinase activity inhibitor, a cell activator, an antiinflammatory agent and a humectant. A vitamin C, a glutathione, a cystine, glabrene, etc., are used as the tyrosinase activator. A deoxyribonucleic acid, adenosine triphosphate, a ribonucleic acid, etc., are used as the cell activator. Glycyrrhizinic acid, mefenamic acid, indomethacin, ibuprofen, etc., are used as antiinflammatory agent. Hyaluronic acid, chondroitinsulfuric acid, dermatan sulfate, etc., are used as the humectant. The content of the dermatan sulfate is about 0.00001-1wt.% calculated as a dried solid content. The amount of the tyrosinase activator inhibitor blended is about 0.0001-10wt.% calculated as a dried solid content, that of the cell activator is about 0.001-5wt.% and that of the antiinflammatory agent is about 0.0001-5wt.%.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition suitable for external use, and more particularly to a composition suitable for external use, such as a cosmetic product or a pharmaceutical product for external use, in which a moss extract and a medicinal agent are blended.

[0002]

2. Description of the Related Art Conventionally, cosmetics such as milky lotions, creams, lotions, packs, dispersions, and detergents, and external medicines such as ointments, creams, and liquids for external use have a predetermined medicinal effect. Medicinal ingredients have been added for this purpose. For example, skin blackening and irritation caused by sunburn,
In order to prevent phenomena such as spots and freckles caused by pigmentation, calamine and the like, ascorbic acids, glutathione, colloidal sulfur, hydroquinone, cinamic aldehyde, and the like are blended. In addition to activation, treatment of wounds after cuts and shave, treatment of wounds such as improvement of cracks, irritations, sores, hemorrhoids, burns, etc., allantoin, aloe extract, ginseng extract, sicon extract, placenta extract, It contains medicinal ingredients such as bovine blood deproteination products and fermentation metabolites.

[0003]

However, compositions suitable for external use such as cosmetics and external medicines containing these medicinal ingredients (hereinafter sometimes referred to as "external compositions").
Then, the effect of the medicinal ingredient is not enough, or
In many cases, the desired medicinal effect cannot be obtained due to deterioration in the preparation, and such improvement has been desired.

[0004]

Means for Solving the Problems The present inventors have conducted intensive studies to improve the effect of the medicinal component of the composition for external use. As a result, when the extract of Kokahi is combined with a specific medicinal agent,
The present inventors have found that the action originally possessed by a medicinal agent is sufficiently exhibited, and completed the present invention.

That is, the present invention relates to the following components (A) and (B): (A) extract of Kokahi (B) an active agent selected from the group consisting of tyrosinase activity inhibitor, cell activator, anti-inflammatory agent and humectant. It is intended to provide a composition containing one or more kinds.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The extract of Kokahi, which is the component (A) of the present invention, is not particularly limited as to the source plant as long as the name of the crude drug is five skins (references; (Lower) p.136-138, Nursery Company). For example, Acanthopanax gracilistylus WW SMITH, A. g
iraldii HARMS, A.sessiliflorus SEEM, A.senticosus
Root bark or stem bark such as HARM (Eleuthero) can be used. The preparation method is not particularly limited, and extraction is performed at low temperature or at room temperature to heating using various appropriate solvents.

As the extraction solvent, for example, one or more of water; lower monohydric alcohols such as methyl alcohol and ethyl alcohol; and liquid polyhydric alcohols such as glycerin, propylene glycol and 1,3-butylene glycol are used. be able to. Examples of the extraction method include a method of chopping or pulverizing the original plant, adding an extraction solvent, immersing and extracting as it is, and optionally performing concentration under reduced pressure, centrifugation, and filtration. As an example of a preferable extraction method, extraction was performed at a low temperature for 10 to 30 days using 1,3-butylene glycol or ethyl alcohol having a water content of 20 to 70% (v / v), followed by filtration. There is a method in which the filtrate is left to age for about 10 days and then filtered again.

[0008] The content of the extract of Kokahi in the composition of the present invention is preferably 0.00001 to 1 as a dry solid content.
% By weight (hereinafter simply referred to as “%”), preferably 0.001 to 20% by weight as an extract,
More preferably, it is in the range of 0.01 to 10%. Within this range, the effects of the tyrosinase activity inhibitor, cell activator, anti-inflammatory agent and humectant can be increased additively or synergistically.

On the other hand, the pharmaceutically active ingredient of the component (B) of the present invention is selected from a tyrosinase activity inhibitor, a cell activator, an anti-inflammatory agent and a moisturizing agent. The following are mentioned.

(Tyrosinase activity inhibitor) Examples of the tyrosinase activity inhibitor include vitamin C and its derivatives and salts thereof, glutathione and its derivatives and their salts, cysteine and its derivatives (for example,
N, N'-diacetylcystine dimethyl, etc.) and salts thereof, glabrene, liquiritin, isoliquiritin, and liquorice extract, calyx extract, soybean extract, eucalyptus extract, Ibukitlano extract, Clara extract, hawthorn extract containing these Extracts, white lily extracts, hop extracts, wild rose extracts, yoquinin extracts, molasses extracts, brown sugar extracts and the like.

Among the tyrosinase activity inhibitors, particularly preferred are vitamin C and its derivatives and salts thereof, cysteine and its derivatives (for example,
N, N'-diacetylcystine dimethyl) and its salts, glabrene, liquiritin, isoliquiritin, and liquorice extract containing these.

(Cell activator) Examples of the cell activator include deoxyribonucleic acid and salts thereof, adenylic acid derivatives such as adenosine triphosphate and adenosine monophosphate and salts thereof, ribonucleic acid and salts thereof, guanine and xanthine And nucleic acid-related substances such as their derivatives and their salts; serum-derived protein extracts, spleen extracts, placenta extracts, cockscomb extracts, royal jelly and other animal-derived extracts; yeast extracts, lactic acid bacteria extracts, bifidobacteria Extracts derived from microorganisms such as fungal extract; carrot extract, assembly extract, ginkgo extract, rosemary extract, oak extract,
Plant-derived extracts such as pentagonal extract, garlic extract, litchi extract, cepharanthin; α- or γ-linolenic acid, eicosapentaenoic acid and derivatives thereof,
Examples thereof include succinic acid and its derivatives and their salts, α-hydroxy acids such as glycolic acid, lactic acid, malic acid, and salicylic acid and their derivatives and their salts.

Of these cell activators, particularly preferred are deoxyribonucleic acid and its salts, adenosine triphosphate and its salts, serum deproteinized extract, placenta extract,
Examples include yeast extract, carrot extract, lactic acid, succinic acid and derivatives thereof, and salts thereof.

(Anti-inflammatory agents) Examples of anti-inflammatory agents include glycyrrhizic acid, mefenamic acid, phenylbutazone, indomethacin, ibuprofen, ketoprofen, guaiazulene and derivatives thereof and salts thereof, ε-aminocaproic acid, zinc oxide, diclofenac sodium , Aloe extract, siconium extract, salvia extract, arnica extract, chamomile extract, birch extract, hypericum extract, eucalyptus extract, mukuroji extract and the like.

Among these anti-inflammatory agents, particularly preferred are glycyrrhizic acid, guaiazulene and their derivatives and salts thereof, ε-aminocaproic acid, aloe extract, sicon extract and chamomile extract.

(Humectant) Examples of the humectant include mucopolysaccharides such as hyaluronic acid, chondroitin sulfate, dermatan sulfate, heparan sulfate, heparin and keratan sulfate and salts thereof, proteins such as collagen, elastin, keratin and derivatives thereof. And salts thereof, sorbitol, inositol, trehalose, urea, pyrrolidonecarboxylic acid and salts thereof.

Of these humectants, particularly preferred are constituents of the skin, such as hyaluronic acid, chondroitin sulfate, dermatan sulfate, heparan sulfate, heparin and keratan sulfate, which are conventionally incorporated in cosmetics. Sugars and their salts, collagen, elastin,
Proteins such as keratin and derivatives thereof and salts thereof. The origin of each of these components is not particularly limited, and may be any of animal origin, microorganism origin, and synthetic products. There are no particular restrictions on the production method, such as the extraction method and purification method.

The amount of the pharmaceutically active ingredient (B) to be added to the composition of the present invention varies depending on the type of the pharmaceutically active ingredient, but is preferably in the following range. Within this range,
When it is combined with the extract of Kokahi of the component (A), it is possible to sufficiently exhibit the inherent effects of the medicinal agent.

That is, the compounding amount of the tyrosinase activity inhibitor in the composition of the present invention is preferably 0.0001 to 10% as a dry solid content, more preferably 0.01 to 10%.
The range is from 01 to 5%. Within this range, a composition exhibiting a superior tyrosinase activity inhibitory effect than when used alone with the tyrosinase activity inhibitor and having a good feeling in use can be obtained.

The amount of the cell activator is preferably 0.001 to 5%, more preferably 0.05%, from the viewpoint of stability over time.
The content is in the range of 1 to 3%, and within this range, a composition having an excellent cell activating effect and an excellent feeling upon use can be obtained.

Further, the compounding amount of the anti-inflammatory agent which exhibits excellent anti-inflammatory effect and obtains a composition having excellent feeling upon use is preferably in the range of 0.0001 to 5%. More preferably, the range is 0.01 to 3%.

Further, mucopolysaccharides as humectants and / or
Alternatively, the amount of the protein in the composition of the present invention is preferably 0.0001 to 5%, more preferably 0.001 to 3%.
% Range. Within this range, a more excellent moisturizing effect is exhibited, and a composition having an excellent feeling upon use can be obtained.

These tyrosinase activity inhibitors, cell activators, anti-inflammatory agents and humectants can be used alone or in combination of two or more.

The composition of the present invention is prepared by blending the essential components (A) and (B) with various types of bases known as ordinary external compositions according to a conventional method. Can be.

Examples of the form of the composition are not particularly limited, and include, for example, emulsions, creams, lotions, packs, dispersions, cleaning agents, makeup cosmetics, cosmetics such as scalp and hair products, ointments, Creams, pharmaceuticals such as liquids for external use and the like can be used as the base of the composition for external use, bases according to the form of these external preparations, for example, purified water, lower alcohols, polyhydric alcohols, fats and oils, Activator, powder,
UV absorbers, thickeners, pigments, antioxidants, preservatives, fragrances, and the like can be used.

[0026]

EXAMPLES Next, the present invention will be described in more detail with reference to Reference Examples, Test Examples and Examples, but the present invention is not limited thereto.

REFERENCE EXAMPLE 1 Production of Gokahi Extract 1 kg of Gokahi is crushed, 10 l of 50% (v / v) ethyl alcohol is added, and the mixture is immersed at room temperature for 15 days and filtered. The filtrate was allowed to stand in a cool and dark place for 5 days, and then filtered to obtain a Kokahi extract.

Reference Example 2 Production of Plant Extract 7 g each of Licorice (JP), Souhakuhi (JP), Kujin (Clara) (JP), and Touki (JP)
100 ml of 0% (v / v) ethyl alcohol was added, extracted at room temperature with occasional stirring for 3 days, and filtered to obtain each plant extract.

Test Example 1 Tyrosinase Activity Inhibition Test The tyrosinase activity inhibition ratio of the extract of Kokahi obtained in Reference Example 1 and the plant extract obtained in Reference Example 2 was examined singly or in combination with each other by the following method. .
That is, an enzyme solution [Sigma, about 28.0
100 units of tyrosinase 10 mg dissolved in 0.1 M phosphate buffer (pH 6.8) 20 ml] 0.1 ml
And 0.1 M phosphate buffer (pH 6.8) was added to make 4.0 ml, which was incubated at 25 ° C. for 10 minutes.

Next, the substrate solution [L-DOPA (Tokyo Kasei) 198.
0 mg in 0.1 M phosphate buffer (pH 6.8) 100 m
dissolved in 1], and reacted for 10 minutes. After the reaction, absorbance at 475 nm (ODS)
Was measured. Similarly, the absorbance (ODHE) when the reaction was performed using the enzyme that had been inactivated by heating and the absorbance (ODB) when no sample was added were measured, and the activity inhibition rate of tyrosinase activity was calculated from Equation 1.

[0031]

(Equation 1)

(Result)

[Table 1]

As is evident from Table 1, the combination of the extract of Kokahi and the inhibitor of tyrosinase activity has a higher tyrosinase activity inhibitory effect than the use of the extract of Kokahi alone or the inhibitor of tyrosinase activity, and is synergistic. A whitening effect was exhibited. Therefore, the composition of the present invention in which the extract of Kokahi and the tyrosinase activity inhibitor are combined, by applying the composition to the skin, exerts an extremely excellent tyrosinase activity inhibitory action, such as skin blackening due to sunburn, spots, freckles, etc. Is effectively suppressed.

Example 1 Cream: A cream was prepared by the composition shown in Table 2 and by the following method, and its skin effect was examined. The results are also shown in Table 2.

(Composition and results)

[Table 2]

(Production method) A. Components (1)-(7), (11) and (12) are mixed and heated to 70 ° C. B. Components (8)-(10) and (13) are mixed and heated to 70 ° C. C. B was added to A, mixed, and cooled to obtain a cream.

(Test method) 27 per test cream
A panel consisting of 15 women aged 54 to 54
After washing the face for 12 weeks, an appropriate amount of the test cream was applied to the face. The beautiful skin effect of the application was evaluated according to the following criteria.

(Evaluation Criteria) <Evaluation> <Contents> Effective The dullness of the skin became inconspicuous. Slightly effective The dullness of the skin became less noticeable. Ineffective No change from before use.

As shown in the results of Table 2, the composition of the present invention obtained by combining the extract of Kokahi represented by the present invention 1 with N, N'-diacetylcystine dimethyl and the extract of Kokahi represented by the present invention 2 It has been clarified that the composition of the present invention, in which a substance and a liquorice extract are combined, can prevent and improve the occurrence of "dullness" or the like of the skin by applying them to the skin, and provide a beautiful skin. .

Example 2 Lotion: (Formulation) (%) (1) Glycerin 5.0 (2) 1,3-butylene glycol 6.5 (3) Polyoxyethylene (20EO) Sorbitan 1.2 Monolauric acid ester (4) Ethyl alcohol 8.0 (5) Gokahi extract * 1 1.0 (6) Magnesium ascorbic acid phosphate * 2 0.5 (7) Preservatives proper amount (8) Fragrance proper amount (9) Remaining purified water * 1 Produced in Reference Example 1 * 2 Takeda Pharmaceutical

(Production method) Components (3), (4), (7) and (8) are mixed and dissolved. B. Components (1), (2), (5), (6) and (9) are mixed and dissolved. C. A and B were mixed and made uniform to obtain a lotion.

Example 3 Emulsion: (Formulation) (%) (1) Polyoxyethylene (10EO) Sorbitan 1.0 Monostearate (2) Polyoxyethylene (60EO) Sorbit 0.5 Tetra Oleate (3) Glyceryl monostearate 1.0 (4) Stearic acid 0.5 (5) Behenyl alcohol 0.5 (6) Squalane 8.0 (7) Gokahi extract * 1 5.0 (8) Licorice extract Product * 2 0.1 (9) Preservative 0.1 (10) Carboxyvinyl polymer 0.1 (11) Sodium hydroxide 0.05 (12) Ethyl alcohol 5.0 (13) Purified water Balance (14) Perfume Appropriate amount * 1 Manufactured in Reference Example 1 * 2 Maruzen Pharmaceutical

(Production method) The components (8) to (13) are mixed by heating and maintained at 70 ° C. B. The components (1) to (6) are mixed by heating and kept at 70 ° C. C. Add A to B, mix and emulsify uniformly. D. After cooling C, (7) and (14) were added and uniformly mixed to obtain an emulsion.

Example 4 Ointment: (Formulation) (%) (1) Stearic acid 18.0 (2) Cetanol 4.0 (3) Triethanolamine 2.0 (4) Glycerin 5.0 (5) Extraction Product * 1 1.0 (6) Saw whisker extract * 2 0.1 (7) Purified water balance * 1 Manufactured in Reference Example 1 * 2 Manufactured in Reference Example 2

(Production method) A part of components (3), (4) and (7) are mixed by heating and kept at 75 ° C. B. Heat mix components (1) and (2) and maintain at 75 ° C. C. Add A slowly to B. D. C was dissolved in the remainder of (7) while cooling (5),
(6) was added to obtain an ointment.

The lotion of Example 2, the lotion of Example 3, and the ointment of Example 4 are all excellent in stability over time, and can be applied to the skin to prevent the occurrence of "dullness" on the skin, Pigmentation such as spots could be improved, and the skin had a clear and beautiful appearance.

Test Example 2 Wound Healing Test Wistar male rats, 8 weeks old, were subjected to the experiment in groups of 10 rats. After shaving the back of the rat, under anesthesia, two left and right back skins were incised over a length of 4 cm so as to be symmetrical to the midline. One was used as a drug application site and the other was used as a control site. Immediately after the incision, three incision sites were sutured and wiped with ethanol for disinfection. Samples 10 to 16 shown in Table 3 were applied to the drug-applied site of the suturing portion.
(One dissolved in physiological saline)
Physiological saline was given to the control site by O.D. 1 ml twice a day, 1
It was applied over a week.

One week later, the skin on the back was peeled off to form a strip-shaped section centering on the incision, and the tensile strength of the skin section was measured using a rheometer NRM-2002J (manufactured by Fudo Kogyo Co., Ltd.). From the obtained measured values, the wound healing rate was calculated by Expression 2. The results are also shown in Table 3.

[0049]

(Equation 2)

[0050]

[Table 3]

As shown in the results in Table 3, when the composition of the present invention in which the extract of Kokahi and a cell activator such as ATP, placenta extract and yeast extract were applied to the skin, the wound healing rate was low. It was clear that it was possible to effectively improve and cure skin trauma, cracks, irritations and the like.

Example 5 Cream: A cream was prepared by the composition shown in Table 4 and by the following method, and its antiaging effect on the skin was examined. Table 4 also shows the results.

(Composition and results)

[Table 4]

(Production method) Components (1)-(7), (11) and (12) are mixed and heated to 70 ° C. B. Components (8)-(10) and (13) are mixed and heated to 70 ° C. C. B was added to A, mixed, and cooled to 30 ° C. to obtain a cream.

(Test method) 26 to 5 per test emulsion
A panel of 15 0-year-old women, twice daily, morning and night,
After washing the face for 12 weeks, an appropriate amount of the test emulsion was applied to the face. The antiaging effect of the skin by application was evaluated according to the following criteria.

Skin Aging Prevention Effect: [Evaluation] [Contents] Effective Skin peeling and gloss were improved. Slightly effective Skin glue, gloss slightly improved. Ineffective No change from before use.

As shown in the results of Table 4, the composition of the present invention comprising the extract of Kokahi typified by Products 3 and 4 of the present invention and a cell activator was applied to the skin to It has been found that the beam and gloss are improved and prevent skin aging.

Example 6 Lotion: (Formulation) (%) (1) Polyoxyethylene (60EO) hydrogenated castor oil 1.0 (2) Ethyl alcohol 15.0 (3) Preservative 0.1 ( 4) ATP * 1 0.01 (5) Appropriate amount of perfume (6) Kokahi extract * 2 2.0 (7) Citric acid 0.1 (8) Sodium citrate 0.3 (9) 1,3-butylene glycol 4.0 (10) Remaining amount of purified water * 1 Wako Pure Chemical Industries * 2 Manufactured in Reference Example 1

(Production method) A. Components (1) to (5) are mixed and dissolved. B. Components (6) to (10) are mixed and dissolved. C. A and B were mixed to obtain a lotion.

Example 7 Emulsion: (Formulation) (%) (1) Polyoxyethylene (10EO) Sorbitan 1.0 Monostearate (2) Polyoxyethylene (60EO) Sorbit 0.5 Tetra Oleate (3) Glyceryl monostearate 1.0 (4) Stearic acid 0.5 (5) Behenyl alcohol 0.5 (6) Squalane 8.0 (7) Eicosapentaenoic acid * 1 3.0 (8) Preservative 0.1 (9) Scarf extract * 2 0.1 (10) Carboxyvinyl polymer 0.1 (11) Sodium hydroxide 0.05 (12) Ethyl alcohol 5.0 (13) Purified water Remaining amount (14) Appropriate amount of fragrance * 1 Sigma * 2 Manufactured in Reference Example 1

(Production Method) The components (10) to (13) are mixed by heating and kept at 70 ° C. B. The components (1) to (8) are mixed by heating and kept at 70 ° C. C. Add A to B, mix and emulsify uniformly. D. After cooling C, (9) and (14) were added and mixed uniformly to obtain an emulsion.

Both the lotion of Example 6 and the emulsion of Example 7 are excellent in stability over time, and when applied to the skin,
It was intended to improve skin adhesion and luster and prevent skin aging.

Example 8 Ointment: (Formulation) (%) (1) Stearic acid 18.0 (2) Cetanol 4.0 (3) Triethanolamine 2.0 (4) Glycerin 5.0 (5) Extraction Product * 1 1.0 (6) Lactic acid * 2 0.1 (7) Remaining purified water * 1 Manufactured in Reference Example 1 * 2 Wako Pure Chemical Industries

(Production Method) A part of components (3), (4) and (7) are mixed by heating and kept at 75 ° C. B. Heat mix components (1) and (2) and maintain at 75 ° C. C. Add A slowly to B. D. C was dissolved in the remainder of (7) while cooling (5),
(6) was added to obtain an ointment.

The ointment of Example 8 was excellent in stability over time and effectively improved skin trauma, cracks, cracks, sores, hemorrhoids, burns and the like.

Test Example 3 Test for Anti-Inflammatory Effect and Pigmentation Inhibiting Effect The drug shown in Table 5 and ethyl alcohol 15% were contained.
A test drug consisting of purified water in the remainder was prepared, applied to the back of a colored guinea pig, and examined for its effects on inflammation and pigmentation due to sunburn. Table 6 shows the results.

(Test method) Colored guinea pig (10 per group)
) Were shaved and irradiated with ultraviolet light under anesthesia. Ultraviolet irradiation is performed by using FL20S / BLB lamp manufactured by Toshiba Corporation and F
Three L20S.E30 lamps were simultaneously irradiated, and the amount of ultraviolet light was 4.8 × 10 4 erg / cm 2. The sample was thoroughly rubbed 0.2 ml each in four places on the back of the guinea pig 24 hours before, immediately after, 12 hours after and 24 hours after the ultraviolet irradiation. The application site was thoroughly washed with warm water before ultraviolet irradiation. The degree of inflammation was observed 24 hours after irradiation and the degree of pigmentation 7 days later.

[0067]

[Table 5]

(Evaluation Criteria) Evaluation Criteria for Inflammation [Score] [State] 0: No inflammation is observed at all 1: Very slight inflammation is observed 2: Inflammation is observed but the boundary between non-irradiated sites is unclear 3: Inflammation is observed, and the boundary with the non-irradiated part is clear

Evaluation Criteria for Pigmentation [Score] [State] 0: No pigmentation is observed 1: Very slight pigmentation is observed 2: Pigmentation is observed, but the boundary between non-irradiated sites is unclear 3: Pigmentation was observed, and the boundary with the non-irradiated part was clear

According to the above evaluation criteria, each score is 1
Count how many out of 10 guinea pigs are below the score,
The determination was made according to the following criteria.

(Judgment Criteria) <Judgment> <Contents> Excellent Effect The number of guinea pigs with a score of 1 or less out of 10 is 8 or more. Effectiveness The number of guinea pigs with a score of 1 or less out of 10 is 6 to 7. Moderately effective Among 10 animals, the number of guinea pigs with a score of 1 or less is 4 to 5 animals. Ineffective Out of 10 animals, the number of guinea pigs with a score of 1 or less is 3 or less.

[0072]

[Table 6]

From the results shown in Table 6, it can be seen that the composition of the present invention containing the extract of Kokahi and the anti-inflammatory agent of dipotassium glycyrrhizinate, guaiazulene, and siconium extract was applied to the skin to give skin irritation and associated irritation. It was revealed that pigmentation was suppressed.

Example 9 Cream: A cream was prepared by the following formulation and the following method. (Prescription) (%) (1) Polyoxyethylene (40EO) monostearate 2.0 (2) Glycerin monostearate (self-emulsifying type) 5.0 (3) Stearic acid 5.0 (4) Behenyl alcohol 0.5 (5) squalane 15.0 (6) cetyl isooctanoate 5.0 (7) butyl paraben 0.1 (8) methyl paraben 0.1 (9) 1,3-butylene glycol 5.0 (10) Dipotassium glycyrrhizinate * 1 0.2 (11) Gokahi extract * 2 0.5 (12) Birch extract * 3 0.1 (13) Remaining purified water (14) Perfume appropriate amount * 1 Tokyo Chemicals * 2 Manufactured in Reference Example 1 * 3 Maruzen Pharmaceutical Co., Ltd.

(Production Method) The components (1) to (7) are heated and dissolved at 70 ° C. B. Heat components (8)-(13) to 70 ° C. C. Add A to B. D. Add component (14) to C and cool to obtain a cream.

Example 10 Emulsion: An emulsion was prepared according to the following formulation and the following production method. (Prescription) (%) (1) Polyoxyethylene (10EO) sorbitan 1.0 monostearate (2) Polyoxyethylene (60EO) sorbit 0.5 tetraoleate (3) Glyceryl monostea Rate 1.0 (4) Stearic acid 0.5 (5) Behenyl alcohol 0.5 (6) Purified avocado oil 4.0 (7) Glyceryl tri-2-ethylhexanoate 4.0 (8) Butylparaben 0.1 ( 9) Gokahi extract * 1 1.0 (10) Aloe extract * 2 0.2 (11) Methyl paraben 0.1 (12) Carboxyvinyl polymer 0.07 (13) 1,3-butylene glycol 5.0 ( 14) Remaining amount of purified water (15) Sodium hydroxide 0.025 (16) Purified water 7.5 (17) Perfume appropriate amount * 1 Produced in Reference Example 1 * 2 Maruzen Company made

(Production Method) The components (1) to (8) are heated and dissolved at 70 ° C. B. Components (9) to (14) are heated and dissolved at 70 ° C. C. Add A to B and emulsify. D. Add (15) to (17) to C and cool to obtain an emulsion.

Example 11 Lotion: A lotion was prepared according to the following formulation and the following method. (Prescription) (%) (1) Polyoxyethylene (60EO) hydrogenated castor oil 1.0 (2) Perfume appropriate amount (3) Ethanol 10.0 (4) Methyl paraben 0.1 (5) Chamomile extract * 1 1.0 (6) Citric acid 0.1 (7) Sodium citrate 0.3 (8) 1,3-butylene glycol 5.0 (9) Gokahi extract * 2 0.01 (10) Purified water residue * 1 Manufactured by Ichimaru Falcos * 2 Manufactured in Reference Example 1

(Production method) The components (1) to (4) are mixed and dissolved. B. Components (5) to (10) are mixed and dissolved. C. Add B to A and stir to obtain a lotion.

The cream of Example 9, the emulsion of Example 10,
Each of the lotions of Example 11 is excellent in stability over time, and by applying to the skin, suppresses dullness of the skin, roughness and the like,
It had an effect of making beautiful skin, and also had an excellent effect of preventing inflammation and pigmentation after sunburn.

Example 12 Hair tonic: A hair tonic was prepared according to the following formulation and the following production method. (Prescription) (%) (1) Kokahi extract * 1 10.0 (2) Chamomile extract * 2 0.3 (3) Menthol 0.1 (4) Ethanol 40.0 (5) Perfume appropriate amount (6) Remaining purified water * 1 Produced in Reference Example 1 * 2 Manufactured by Ichimaru Falcos

A. Components (3) to (5) are mixed and dissolved. B. Components (1), (2) and (6) are mixed and dissolved. C. A was added to B and mixed uniformly to obtain a hair tonic.

The hair tonic of Example 12 was applied to the scalp to suppress dandruff and Kayumi caused by scalp inflammation.
It had beautiful scalp and hair.

Example 13 Emulsion: An emulsion was prepared according to the composition shown in Table 7 and the following method, and the effect of improving skin roughness was examined. Table 7 also shows the results.

(Composition and Results)

[Table 7]

(Production Method) Each component of (6) to (10) and (14) is heated and mixed and kept at 70 ° C. B. The components (1) to (5), (11) and (12) are mixed by heating and maintained at 70 ° C. C. The above B was added to the above A and mixed, and the component (13) was added to emulsify uniformly, followed by cooling to 30 ° C. to obtain an emulsion.

(Test Method) Using a panel of 15 healthy persons aged 24 to 45 years old, the skin condition before causing experimental rough skin was photographed with a microscope camera, and the score was obtained according to the following criteria. Experimental rough skin was caused by treating the upper arm flexion side with a mixture of ether and acetone (1: 1). Thereafter, the test emulsion was applied twice a day, in the morning and at night, for 7 days, and the scores of the skin condition were obtained in the same manner as described above 1, 3, 5 and 7 days after the rough skin was induced.

Skin Condition Score <Score> <State> 1 Skin groove of skin is unclear and exfoliation of keratin is recognized. 2 Skin crevices are slightly unclear or strongly unidirectional. 3 Skin sulcus is recognized but shallow or strongly unidirectional. 4 Skin sulcus is recognized or slightly reticulated. 5 Skin crevices are clearly recognized or have a fine mesh.

As shown in the results of Table 7, the composition of the present invention obtained by combining the extract of Kokahi typified by the product 5 of the present invention with mucopolysaccharide, and the extract combined with the extract of Kokahi typified by the product 6 of the present invention Further, it has been clarified that the composition of the present invention can improve rough skin by applying these compositions to the skin.

Example 14 Cream: An emulsion was prepared according to the composition shown in Table 8 and the following method, and its skin effect was examined. The results are also shown in Table 8.

(Composition and results)

[Table 8]

(Production Method) Components (1)-(7), (11) and (12) are mixed and heated to 70 ° C. B. Components (8)-(10) and (13) are mixed and heated to 70 ° C. C. B was added to A, mixed, and cooled to obtain a cream.

(Test method) 27 per test cream
A panel consisting of 15 women aged 54 to 54
After washing the face for 12 weeks, an appropriate amount of the test cream was applied to the face. The beautiful skin effect of the application was evaluated according to the same criteria as in Example 1.

As shown in the results of Table 8, the composition of the present invention obtained by combining the extract of Kokahi represented by the product 7 of the present invention with mucopolysaccharide and the combination of the extract of Kokahi represented by the product 8 of the present invention and a protein By applying these compositions to the skin, the composition of the present invention prepares the texture of the skin, keeps the moisture content of the skin at an appropriate level, and prevents and improves the occurrence of "dullness" and the like on the skin. It became clear that.

Example 15 Lotion: A lotion was prepared according to the following formulation and the following method. (Prescription) (%) (1) Glycerin 5.0 (2) 1,3-butylene glycol 6.5 (3) Polyoxyethylene (20EO) sorbitan 1.2 Monolaurate (4) Ethyl alcohol 8 0.0 (5) Kokahi extract * 1 1.0 (6) Collagen * 2 0.5 (7) Appropriate amount of preservative (8) Appropriate amount of fragrance (9) Remaining amount of purified water * 1 Produced in Reference Example 1 * 2 manufactured by Koken

(Production method) Components (3), (4), (7) and (8) are mixed and dissolved. B. Components (1), (2), (5), (6) and (9) are mixed and dissolved. C. A and B were mixed and made uniform to obtain a lotion.

Example 16 Pack: A pack was prepared according to the following formulation and the following production method. (Prescription) (%) (1) Polyvinyl alcohol 20.0 (2) Ethyl alcohol 20.0 (3) Glycerin 5.0 (4) Kaolin 6.0 (5) Gokahi extract * 1 10.0 (6) Sodium hyaluronate * 2 0.5 (7) Preservative appropriate amount (8) Fragrance 0.05 (9) Purified water Remaining amount * 1 Produced in Reference Example 1 * 2 Made by Kewpie

(Production method) Mixing the components (1), (3) to (6) and (9),
Heat to 70 ° C. and stir. B. Mix components (2), (7) and (8). C. The above B was added to the above A, mixed, and then cooled to obtain a pack.

Both the lotion of Example 15 and the pack of Example 16 were excellent in stability over time. By applying them to the skin, the texture of the skin was adjusted, the moisture content of the skin was maintained at an appropriate level, and the It was possible to prevent and improve the occurrence of "dullness" and the like, and also to improve the skin shine and gloss to make the skin beautiful.

Example 17 Cleaning agent: A cleaning agent was prepared according to the following formulation and the following production method. (Prescription) (%) (1) Stearic acid 10.0 (2) Palmitic acid 8.0 (3) Myristic acid 12.0 (4) Lauric acid 4.0 (5) Oleyl alcohol 1.0 (6) Purification Lanolin 1.0 (7) Fragrance 0.1 (8) Preservative 0.2 (9) Glycerin 18.0 (10) Potassium hydroxide 6.0 (11) Gokahi extract * 1 0.5 (12) Keratin Hydrolyzed liquid * 2 0.05 (13) Remaining purified water * 1 Produced in Reference Example 1 * 2 Manufactured by Seiwa Kasei

(Production method) Mix components (9), (10) and (13) and mix
Heat to ° C. B. Mix components (1)-(6) and (8) and heat to 70 ° C. C. The above B was added to the above A, the components (7), (11) and (12) were added, and the mixture was cooled to obtain a cleaning agent.

The cleaning agent of Example 17 has excellent stability over time,
In addition, there was no feeling of firmness after rinsing, and it was excellent in moisturizing properties.

[0102]

According to the present invention, it is possible to sufficiently exert the inherent effects of a medicinal agent. For example, since the composition of the present invention containing a tyrosinase activity inhibitor as a medicinal agent has a stable and excellent whitening effect, it exerts a high inhibitory effect on pigmentation of the skin, and in particular, the "dullness" of the skin due to pigmentation Is excellent in prevention and improvement effects.
In addition, the composition of the present invention containing an anti-inflammatory agent exhibits a high inhibitory effect on inflammation of the skin and pigmentation accompanying it, so that the skin becomes rough due to sunburn, blackening of the skin,
It is effective in preventing and improving spots and freckles. On the other hand, the composition of the present invention containing a cell activator as a medicinal agent has an excellent cell activator, preventing skin aging, trauma,
It is effective in improving cracks, irritations, sores, hemorrhoids, burns, etc., and promoting wound healing. Furthermore, since the composition of the present invention containing a humectant as a medicinal agent has a stable and excellent skin roughness improving action and moisturizing action, it prevents and improves skin `` dullness '' etc. It is provided effectively. As described above, the composition of the present invention can sufficiently exhibit the intrinsic performance of a medicinal agent, and is extremely useful in beauty and medicine.

Claims (12)

[Claims] 1. One or two of the following components (A) and (B): (A) extract of Kokahi (B) a tyrosinase activity inhibitor, a cell activator, an anti-inflammatory agent and a humectant A composition comprising the above. 2. The composition according to claim 1, wherein the pharmaceutically active agent is a tyrosinase activity inhibitor. 3. The method according to claim 1, wherein the tyrosinase activity inhibitor is vitamin C.
And derivatives thereof, salts thereof, glutathione and derivatives thereof, and salts thereof, cysteine and derivatives thereof, and salts thereof, gravuren, liquiritin, isoliquiritin, and a liquorice extract, a saccharomycin extract, a soybean extract, and a squirrel extract containing them 3. The extract according to claim 2, wherein the extract is selected from the group consisting of: Ibukitrano extract, Clara extract, Hawthorn extract, Shirahuri extract, Hop extract, Neubara extract, Yoquinin extract, molasses extract and brown sugar extract. Composition. 4. The composition according to claim 1, wherein the medicinal agent is a cell activator. 5. The cell activator comprises deoxyribonucleic acid and salts thereof, adenylic acid derivatives such as adenosine triphosphate and adenosine monophosphate and salts thereof, ribonucleic acids and salts thereof, guanine, xanthine and derivatives thereof, and the like. Nucleic acid-related substances such as salt of the protein; extracts derived from animals such as serum deproteinized extract, spleen extract, placenta extract, cockscomb extract, royal jelly; microorganisms such as yeast extract, lactic acid extract, and bifidobacterium extract Extracts derived from plants; plant-derived extracts such as carrot extract, assembly extract, ginkgo extract, rosemary extract, oak extract, ogre extract, garlic extract, litchi extract, cepharanthin; α- or γ-linolenic acid, eicosapentaenoic acid and derivatives thereof, succinic acid and derivatives thereof, salts thereof, and glyco Le acid, lactic acid, malic acid, alpha-hydroxy acids and derivatives thereof, such as salicylic acid, as well as composition of claim 4, wherein a member selected from the salts thereof. 6. The composition according to claim 1, wherein the pharmaceutically active agent is an anti-inflammatory agent. 7. The anti-inflammatory agent is glycyrrhizic acid, mefenamic acid, phenylbutazone, indomethacin, ibuprofen, ketoprofen, guaiazulene and derivatives thereof and salts thereof, ε-aminocaproic acid, zinc oxide, diclofenac sodium, and aloe extract. 7. The composition according to claim 6, wherein the composition is selected from the group consisting of a radish extract, a radish extract, a salvia extract, an arnica extract, a chamomile extract, a birch extract, a hypericum extract, a eucalyptus extract and a sapling extract. 8. The composition according to claim 1, wherein the medicinal agent is a humectant. 9. The humectant is a mucopolysaccharide such as hyaluronic acid, chondroitin sulfate, dermatan sulfate, heparan sulfate, heparin and keratan sulfate and salts thereof, proteins such as collagen, elastin, keratin and derivatives thereof, and salts thereof. 9. The composition according to claim 8, which is selected from sorbitol, inositol, trehalose, urea, pyrrolidonecarboxylic acid and salts thereof. 10. The composition according to claim 1, which is an external preparation for skin. 11. The cosmetic according to claim 1, which is a cosmetic.
The composition according to any one of the above items. 12. The composition according to claim 1, which is a topical drug.