JP5993724B2 - Elastin production promoter and skin cosmetics - Google Patents

Description

  The present invention relates to an elastin production promoter, an agent for preventing / ameliorating skin wrinkling / sagging / tension reduction, and a skin cosmetic.

  The dermis and epidermis of the skin is composed of epidermal cells, fibroblasts, and dermal extracellular matrix such as elastin and collagen that are outside these cells and support the skin structure. In young skin, these skin tissues By maintaining the homeostasis, moisture retention, flexibility, elasticity and the like are ensured, and the skin is maintained in a fresh state with a tension and gloss.

  However, elastin, which is a major component of the dermal extracellular matrix, due to the influence of certain external factors such as ultraviolet irradiation, significant air drying, excessive skin washing, etc. The production amount of collagen and the like is reduced, and degeneration and decomposition are caused. As a result, the stratum corneum begins to exfoliate abnormally, the skin loses its tension and gloss, and exhibits aging symptoms such as rough skin and wrinkles.

  As described above, changes accompanying aging of the skin, that is, formation of wrinkles, disappearance of tension, decrease in elasticity, and the like involve reduction and degeneration of dermal extracellular matrix components such as elastin and collagen.

  Among these matrix components, elastin is a fiber that gives elasticity to skin tissue, and its production amount decreases with aging and the like, and decomposition and degeneration are promoted by ultraviolet rays. When normal elastin is reduced, the elasticity of the skin is reduced, which can cause wrinkles and sagging. Therefore, if the production of elastin can be promoted, wrinkles and sagging are less likely to occur, and skin aging symptoms such as loss of tension and reduced elasticity can be prevented and improved.

  In addition to skin tissue, elastin is widely expressed in tissues that require elasticity in the body, such as lungs and blood vessels. It is known that when normal elastin decreases from these tissues with aging, elasticity in the lungs and blood vessels decreases, causing pulmonary diseases such as emphysema and vascular diseases such as hypertension and aneurysms. ing. Therefore, if the production of elastin can be promoted, it will be difficult for the elasticity of the lungs and blood vessels to decrease, and pulmonary diseases such as emphysema and vascular diseases such as hypertension and aneurysms may be prevented and treated. .

  Conventionally, for example, an extract of a buckwheat is known as one having an elastin production promoting action (see Patent Document 1). However, it is difficult to provide a substance having an elastin production promoting action, and there is a strong demand for the development and provision of further new elastin production promoting substances.

  Conventionally, for bitter gourd, it has been proposed to blend an extract from the fruit portion into cosmetics (see Patent Document 2), but the bitter gourd stem, leaf, or an extract thereof is made up. It has not been proposed for use in fees.

JP 2012-056933 A Japanese Patent Laid-Open No. 2002-212050

  The present invention finds an elastin production promoting action or a skin wrinkle / sagging / stretching prevention / amelioration action among highly safe natural products, and an elastin production promoter and skin containing the same as an active ingredient An object of the present invention is to provide a preventive / improving agent for wrinkles / sagging / tension reduction, and a skin cosmetic containing the natural product.

  In order to solve the above-mentioned problems, the elastin production promoter and the skin wrinkle / sagging / strain reduction / amelioration agent of the present invention contain an extract from bitter gourd stem and / or leaf as an active ingredient. It is characterized by doing. In addition, the skin cosmetic of the present invention is characterized by blending extracts from bitter gourd stems and / or leaves.

  According to the present invention, there is an excellent elastin production promoting action and a highly safe elastin production promoting agent, and an excellent skin wrinkling / sagging / strain reduction / strength reducing / strengthening and safety. It is possible to provide an agent for preventing / ameliorating skin wrinkles / sagging / reducing tension. Furthermore, according to the present invention, it is possible to provide a highly safe skin cosmetic that has an excellent elastin production promoting effect or a preventive / improving effect on the formation of skin wrinkles / sagging / reducing tension.

Embodiments of the present invention will be described below.
The elastin production promoter and the preventive / improving agent for skin wrinkle / sagging / tension reduction according to the present embodiment contain extracts from bitter gourd stems and / or leaves as active ingredients. Moreover, the skin cosmetic of this embodiment is blended with extracts from bitter gourd stems and / or leaves.

  Here, in the present embodiment, the “extract from bitter gourd stalk and / or leaf” refers to an extract obtained by using bitter gourd stalk and / or leaf as an extraction raw material, a diluted solution of the extract or A concentrated liquid, a dried product obtained by drying the extract, or a crudely purified product or a purified product thereof are included.

  In this embodiment, the extraction raw material used for producing the extract from the stem and / or leaf of bitter gourd is bitter gourd (scientific name: Momordica charantia var. Pavel).

  Bitter gourd (Momordica charantia var. Pavel) is an annual herb of vine that belongs to the genus Turresi, and is also known as vine reed or bitter gourd, and its fruit is used for food. Although bitter gourd originates in tropical Asia, it is widely cultivated in various parts of Japan, including the Nansei Islands and South Kyushu, and can be easily obtained from these areas.

  The types of bitter gourd used in the present embodiment are: blown bitter gourd, long litchi, thick litchi, medium long bitter gourd, white litchi, utchabordae, white long litchi, petite litchi, taigo moth, folding screen, group star, medium long bitter melon, longevity There are bitter gourd, vietnam bitter gourd, pure white bitter gourd, tempered bitter gourd, etc., and any kind, bitter gourd, etc. may be used, but long litchi is particularly preferable.

  The constituent parts of bitter gourd used as an extraction raw material in this embodiment are a stem part and / or a leaf part. In the present specification, the “stem portion” includes a vine portion. In addition, bitter gourd is used for food, but when the stem part and / or the leaf part of bitter gourd is collected as an extraction raw material in the present embodiment, it may be performed either before or after fruit collection.

  Extracts from bitter gourd stalks and / or leaves can be obtained by drying the raw material for extraction and then pulverizing it as it is or using a crusher and subjecting it to extraction with an extraction solvent. Drying may be performed in the sun or using a commonly used dryer. Moreover, after performing pretreatment, such as degreasing, with a nonpolar solvent such as hexane, it may be used as an extraction raw material. By performing pretreatment such as degreasing, extraction with polar solvent of bitter gourd stems and / or leaves can be efficiently performed.

  As the extraction solvent, it is preferable to use a polar solvent, and examples thereof include water and hydrophilic organic solvents. These are used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. It is preferable.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, and those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, the water that can be used as the extraction solvent in this embodiment includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of hydrophilic organic solvents that can be used as extraction solvents include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when a mixed liquid of water and lower aliphatic alcohol is used as an extraction solvent, the mixing ratio of water and lower aliphatic alcohol is preferably 9: 1 to 1: 9 (volume ratio), More preferably, it is 7: 3 to 2: 8 (capacity ratio). In addition, when a mixed liquid of water and a lower aliphatic ketone is used, the mixing ratio of water and the lower aliphatic ketone is preferably 9: 1 to 2: 8 (volume ratio). When using the liquid mixture with a monohydric alcohol, it is preferable that the mixing ratio of water and a polyhydric alcohol is 5: 5 to 1: 9 (volume ratio).

  The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted in the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent 5 to 15 times (mass ratio) of the extraction raw material, the soluble components are extracted at room temperature or under reflux, and then filtered to remove the extraction residue. A liquid can be obtained. When the solvent is distilled off from the obtained extract, a paste-like concentrate is obtained, and when this concentrate is further dried, a dried product is obtained.

  The extract obtained as described above can be used as an active ingredient of an elastin production promoter or an agent for preventing / ameliorating skin wrinkles / sagging / strain reduction as it is. It is easier to use.

  In addition, the extract from bitter gourd stalk and / or leaf has a unique odor, so it can be purified for the purpose of decolorization, deodorization, etc. within a range that does not reduce its physiological activity. However, since it is not used in a large amount when blended into skin cosmetics or the like, there is no practical problem even if it is not purified.

[Elastin production promoter, skin wrinkle / sagging / prevention / improvement of tension reduction]
The extract from the stems and / or leaves of bitter gourd obtained as described above has an excellent elastin production promoting effect and the prevention / improving effect of skin wrinkling / sagging / reducing tension, It can be used as an active ingredient of an elastin production promoter or a prophylactic / ameliorating agent for skin wrinkle / sagging / tension reduction. The elastin production promoter and the skin wrinkle / sagging / tension reduction / amelioration agent of the present embodiment can be used in a wide range of applications such as pharmaceuticals, quasi drugs and cosmetics.

  Here, the preventive / improving effect of skin wrinkle / sagging / decrease reduction of the extract from bitter gourd stem and / or leaf is exhibited based on elastin production promoting effect, for example. However, the extract from bitter gourd stalk and / or leaf has the effect of preventing or improving skin wrinkling / sagging / reducing tension on the skin based on the elastin production promoting effect / It is not limited to the prevention / improvement of tension reduction.

  Extracts from bitter gourd stems and / or leaves are used to promote elastin production or to prevent or improve skin wrinkling / sagging / reducing tension, thereby reducing tension and reducing elasticity. It may be used as an active ingredient of an agent for preventing or improving skin aging such as pulmonary disease such as emphysema and prevention of vascular diseases such as hypertension and aneurysm. It can also be used as an active ingredient of a therapeutic agent.

  The elastin production promoter or the skin wrinkle / sagging / tension reduction / improvement agent of the present embodiment may consist only of an extract from bitter gourd stalk and / or leaf, or bitter gourd stalk. And / or an extract from the leaf may be formulated.

  The elastin production promoter of this embodiment or the preventive / improving agent of skin wrinkle / sagging / tension reduction is a pharmaceutically acceptable carrier such as dextrin, cyclodextrin, etc. It can be formulated into any dosage form such as powder, granule, tablet, and liquid. In this case, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring / flavoring agent, and the like can be used. An elastin production promoter or an agent for preventing / ameliorating skin wrinkling / sagging / tension reduction can be used in combination with other compositions (for example, external preparations for skin, cosmetic foods and drinks, etc.) and ointments It can be used as an agent, an external solution, a patch and the like.

  When the elastin production promoter of this embodiment or the prophylactic / improving agent for skin wrinkle / sagging / tension reduction is formulated, the content of the extract from the stem and / or leaf part of bitter gourd is particularly limited. It can be set as appropriate according to the purpose.

  In addition, the elastin production promoter of this embodiment or the prevention / improving agent for skin wrinkle / sagging / tension reduction, if necessary, the elastin production promoting action or the prevention / improvement of skin wrinkle / sagging / tension reduction. Other natural extracts having an action and the like can be blended together with extracts from bitter gourd stems and / or leaves and used as active ingredients.

  Examples of the administration method for the patient of the elastin production promoter or the prevention / amelioration agent of skin wrinkle / sagging / tension reduction of the present embodiment include percutaneous administration, oral administration, etc., depending on the type of the disease, What is necessary is just to select a suitable method for the prevention, treatment, etc. suitably.

  In addition, the dose of the elastin production promoter or the agent for preventing / ameliorating skin wrinkling / sagging / tension reduction according to the present embodiment is also appropriately determined depending on the type of disease, severity, individual patient differences, administration method, administration period, etc. Just increase or decrease.

  The elastin production promoter of the present embodiment prevents and ameliorates skin aging symptoms such as loss of tension and reduced elasticity through elastin production promotion action of extracts from bitter gourd stems and / or leaves. It is also possible to prevent and treat pulmonary diseases such as emphysema and vascular diseases such as hypertension and aneurysms. However, the elastin production promoter of the present embodiment can be used for all purposes that are meaningful for exerting an elastin production promoting action in addition to these uses.

  The agent for preventing / ameliorating skin wrinkling / sagging / tension reduction according to the present embodiment is used for preventing / ameliorating skin wrinkling / sagging / tension reduction of an extract from the stem and / or leaf part of bitter gourd. In addition, it is possible to prevent or improve wrinkles, sagging in the skin, or reduction of tension in the skin. However, the preventive / improving agent for skin wrinkle / sagging / tension reduction according to the present embodiment is significant in exhibiting the effect of preventing / ameliorating skin wrinkle / sagging / tension reduction in addition to these uses. Can be used for all purposes.

  In addition, the elastin production promoter and the skin wrinkle / sag formation / tension reduction / reduction agent of the present embodiment have an excellent elastin production promotion effect and skin wrinkle / sag formation / tension reduction prevention / improvement action. Therefore, for example, it is suitable for blending into a skin external preparation or food and drink. In this case, an extract from bitter gourd stalk and / or leaf may be blended as it is, or an elastin production promoter formulated from an extract from bitter gourd stalk and / or leaf may be used. A prophylactic / improving agent for wrinkle / sagging / tension reduction may be added.

  Here, the external preparation for skin is not limited in its category, and includes a wide range of quasi-drugs and pharmaceuticals used transdermally, in addition to the skin cosmetics described below. Moreover, as food and drink, there is no restriction on the classification, and it includes a wide range of foods such as general foods, health foods, health functional foods and the like taken orally.

  Furthermore, the elastin production promoter and skin wrinkle / sagging / tension reduction / reduction agent of the present embodiment have an excellent elastin production promoting action and skin wrinkling / sagging / tension reduction preventing / improving action. Therefore, it can be suitably used as a reagent for research related to, for example, the production mechanism of elastin, the formation mechanism of skin wrinkles and sagging, and the mechanism of skin tension reduction.

[Skin cosmetic]
Extracts from bitter gourd stalks and / or leaves have excellent elastin production promoting effects and skin wrinkle / sagging / reduction of tension reduction. It is suitable for. In this case, an extract from bitter gourd stalk and / or leaf may be blended as it is, or an elastin production promoter or skin wrinkle formulated from an extract from bitter gourd stalk and / or leaf. -An agent for preventing / ameliorating sagging / tension reduction may be added. By incorporating an extract from bitter gourd stalk and / or leaf, the above elastin production promoter or the above skin wrinkle / sagging / tension reduction preventive / improving agent into the skin cosmetic, elastin is added to the skin cosmetic. It can provide a production promoting effect or a preventive / improving effect on skin wrinkle / sagging / strain reduction.

  Skin cosmetics that can be blended with extracts from bitter gourd stalks and / or leaves, the above elastin production promoter or the above-mentioned skin wrinkle / sagging / tension reduction preventive / improving agent Instead, for example, ointments, creams, emulsions, lotions, packs, foundations, lip balms, bath salts, hair nicks, hair lotions, soaps, body shampoos and the like can be mentioned.

  When an extract from bitter gourd stalk and / or leaf is blended into the skin cosmetic, the blending amount can be adjusted as appropriate according to the type of skin cosmetic, but a suitable blending ratio is about 0. 0.0001 to 10% by mass, a more preferable compounding rate is about 0.001 to 1% by mass, and a further more preferable compounding rate is about 0.0015 to 0.1% by mass. Is about 0.002 to 0.005 mass%.

  The skin cosmetic of the present embodiment is a normal cosmetic unless it prevents the elastin production promoting action or the prevention / improving action of skin wrinkling / sagging / strain reduction of the extract from the stem and / or leaf part of bitter gourd. Main agents, auxiliaries or other components used in the manufacture of skin cosmetics, such as astringents, bactericides / antibacterial agents, whitening agents, UV absorbers, moisturizers, cell activators, anti-inflammatory / anti-allergic agents, antioxidant / Active oxygen scavengers, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances and the like can be used in combination. When used in combination, it becomes a more general product, and a synergistic effect with other active ingredients used in combination may lead to an excellent effect that is more than normally expected.

  The skin cosmetic according to the present embodiment is capable of producing wrinkles and sagging in the skin through the elastin production promoting action or the prevention / amelioration of skin wrinkling / sagging / strain reduction, which the extract from bitter gourd stem and / or leaves has. It is possible to prevent and improve the formation of skin and the decrease in tension in the skin, and to prevent and improve skin aging symptoms such as reduced elasticity.

  Although the elastin production promoter, the skin wrinkle / sagging / tension reduction preventive / improving agent and the skin cosmetic of the present embodiment are suitably applied to humans, their respective effects are As long as it is played, it can also be applied to animals other than humans (for example, mice, rats, hamsters, dogs, cats, cows, pigs, monkeys, etc.).

  Hereinafter, although a manufacture example and a test example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to each following example at all.

[Production Example 1] Manufacture of bitter gourd stalk / leaf extract The bitter gourd stalk and leaf were collected and dried. 100 g of the pulverized product of the dried product was refluxed and extracted with 1500 mL of 50% (v / v) ethanol (80 to 90 ° C., 2 hours), filtered and freeze-dried to obtain a bitter gourd stem / leaf extract. (Solid content 21.83 g, sample 1).

[Test Example 1] Elastin production promoting action test The bitter gourd stalk / leaf extract (sample 1) obtained in Production Example 1 was tested for elastin production promoting action as follows.

Human normal fibroblasts (NB1RGB) were cultured using Dulbecco's MEM medium containing 10% FBS, and then cells were collected by trypsin treatment. The collected cells were diluted with the above medium to a concentration of 2.2 × 10 ⁵ cells / mL, then seeded at 100 μL per well in a 96-well microplate, and cultured overnight. After completion of the culture, the medium was removed, and 0.25% FBS-containing Dulbecco MEM medium added with a test sample (Sample 1, see Table 1 below) or 0.25% FBS-containing Dulbecco MEM medium without the sample added Was added to each well and cultured for 5 days. After completion of the culture, the supernatant was collected, and the amount of elastin released into the culture supernatant was measured as follows.

  100 μL of the supernatant was transferred to an ELISA plate and adsorbed on the plate overnight at 4 ° C., and then the solution was discarded and washed with a phosphate physiological buffer solution (PBS-T) containing 0.05% Tween20. . Thereafter, a blocking operation was performed with PBS-T containing 1% bovine serum albumin. The solution was discarded, washed with PBS-T, and reacted with an anti-human elastin antibody (rabbit IgG, manufactured by NOVOTEC). The solution was discarded, washed with PBS-T, and reacted with a peroxidase-labeled anti-rabbit IgG antibody (manufactured by Nichirei Bioscience). After discarding the solution, the same washing operation was performed to perform a color reaction. From the obtained results, the elastin production promotion rate (%) was calculated by the following formula.

Elastin production promotion rate (%) = A / B × 100
In the formula, A represents “the amount of elastin when the test sample is added”, and B represents “the amount of elastin when no test sample is added”.
The results are shown in Table 1.

  As shown in Table 1, Sample 1 promoted elastin production in a concentration-dependent manner. Therefore, the bitter gourd stalk / leaf extract was recognized as an excellent elastin production promoting action. The bitter gourd stalk / leaf extract is considered to have a preventive / improving effect on skin wrinkle / sagging / tension reduction based on at least the above elastin production promoting effect.

[Formulation Example 1]
A milky lotion was produced by a conventional method according to the following composition.
0.01 g of bitter gourd stalk and leaf extract (Production Example 1)
Jojoba oil 4.00 g
1,3-butylene glycol 3.00 g
Arbutin 3.00g
Polyoxyethylene cetyl ether (20E.O.) 2.50g
2.00 g of olive oil
Squalane 2.00g
Cetanol 2.00g
Glyceryl monostearate 2.00g
Oleic acid polyoxyethylene sorbitan (20E.O.) 2.00g
Methyl paraoxybenzoate 0.15g
Stearyl glycyrrhizinate 0.10g
Twilight extract 0.10g
Dipotassium glycyrrhizinate 0.10g
Ginkgo biloba extract 0.10g
Conchiolin 0.10g
Oat extract 0.10g
Chamomile extract 0.10g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

[Formulation Example 2]
A cream having the following composition was produced by a conventional method.
Bitter melon stem / leaf extract (Production Example 1) 0.05 g
Kujin extract 0.1g
Ogon Extract 0.1g
Liquid paraffin 5.0g
Salami beeswax 4.0g
Squalane 10.0g
Cetanol 3.0g
Lanolin 2.0g
Stearic acid 1.0g
Oleic acid polyoxyethylene sorbitan (20E.O.) 1.5g
3.0 g glyceryl monostearate
Oil soluble licorice extract 0.1g
1,3-butylene glycol 6.0 g
1.5 g of methyl paraoxybenzoate
Fragrance 0.1g
Purified water remainder (total amount is 100 g)

[Composition Example 3]
A serum having the following composition was produced by a conventional method.
0.01 g of bitter gourd stalk and leaf extract (Production Example 1)
Chamomile extract 0.1g
Carrot extract 0.1g
Xanthan gum 0.3g
Hydroxyethylcellulose 0.1g
Carboxyvinyl polymer 0.1g
1,3-butylene glycol 4.0 g
0.1g dipotassium glycyrrhizinate
Glycerin 2.0g
Potassium hydroxide 0.25g
Fragrance 0.01g
Preservative (Methyl paraoxybenzoate) 0.15g
Ethanol 2.0g
Purified water remainder (total amount is 100 g)

  The elastin production promoter, the skin wrinkle / sagging / tension reduction preventive / improving agent and the skin cosmetics of the present invention are used for the prevention / improvement of skin aging symptoms such as loss of tension, reduced elasticity, emphysema, etc. It can greatly contribute to prevention and treatment of vascular diseases such as lung diseases, hypertension, and aneurysms.

Claims (3)

  An elastin production promoter comprising an extract from bitter gourd stalk and / or leaf as an active ingredient.   An agent for preventing / ameliorating skin wrinkle / sagging / tension reduction characterized by containing an extract from bitter gourd stalk and / or leaf as an active ingredient. It is a skin cosmetic used for the prevention / improvement of skin wrinkles / sagging / tension reduction or for the prevention / improvement of skin aging, which contains an extract from the stem and / or leaf part of bitter gourd The skin cosmetics featured.