JPH0640882A - Whitening cosmetic - Google Patents

Abstract

PURPOSE:To provide a cosmetic having excellent melanogenicity-inhibitory activity, useful for removing spots etc. CONSTITUTION:The cosmetic characterized by containing (A) an extract obtained by extracting Momordica charantia with a polar solvent, (B) a liquor obtained by squeezing the plant, or (C) a concentrate therefrom.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a whitening cosmetic composition containing an extract or a squeezed liquid of a spinach and a polar solvent, or a concentrate thereof.

[0002]

2. Description of the Related Art Tsurureishi [Scientific name: Mormodica
charantia L. ] Is an annual vine of the cucumber family that is native to tropical Asia and is also cultivated in Japan, and is also known as ganiuri. It is known that the fruits and seeds of Tsutsurishishi have antipyretic action, detoxification action, analgesic action, and therapeutic action against diarrhea.

On the other hand, conventionally, as a whitening cosmetic having an effect of removing spots on the skin, a preparation containing L-ascorbic acid, arbutin which is a hydroquinone derivative, or kojic acid as its active ingredient has been proposed.

[0004]

[Problems to be Solved by the Invention] Pigmentation of the skin, such as that seen in spots, freckles, and sunburn, is caused by the action of tyrosinase on the tyrosine present in the skin to form melanin, which then migrates to epidermal keratinocytes. It is caused by being taken in. Therefore, if the activity of tyrosinase which is a melanin-producing enzyme in cells is suppressed, it is possible to remove spots, freckles, etc., but the above-mentioned L-ascorbic acid and arbutin are not sufficiently effective in suppressing the tyrosinase activity, and kojic acid is There is a drawback in that it is not suitable for whitening cosmetics in terms of stability.

[0005]

The inventor of the present invention suppresses melanin production by suppressing tyrosinase activity in a liquid obtained by extracting a fruit of Tsurumishi and the like with a polar solvent and a liquid obtained by pressing. The present invention has been completed by discovering that it contains a component exhibiting excellent effects on, and is useful for removing spots on the skin.

The whitening cosmetic composition of the present invention contains an extract or squeeze solution of a succulent with a polar solvent or a concentrate thereof. The extract of Tsurugi is to be added with polar solvent (eg water, methanol, ethanol, acetonitrile, acetone, etc.) to root, stem, flower, leaf, fruit, seed or whole plant of Tsurushi and extract while heating appropriately. Can be obtained by When using the fruit part of the smoothie, after freeze-drying by a conventional method,
It can also be crushed and then extracted. The squeezing liquid of the smoothie can be obtained by squeezing the fruit of the smoothie using a squeezing machine or the like. The extract and the squeezed liquid of the thus-obtained polar tuna sponge can be made into a concentrate by performing vacuum drying, freeze-drying and the like by a conventional method. Here, the concentrate includes both a concentrate and a concentrate dried product.

Furthermore, the concentrate of the extract or squeezed liquid with the polar solvent of Tsutsurishi used in the whitening cosmetic composition of the present invention can be purified by subjecting it to column chromatography and eluting with an aqueous solvent. Examples of the column chromatography used include synthetic adsorbents (for example, HP-20, SP-200, SP-800, HPMG:
Mitsubishi Kasei Co., Ltd., and those with a filler such as alumina can be used. Examples of the solvent used for elution include aqueous solvents such as water and hydroalcohol (for example, hydrous methanol, hydrous ethanol, etc.).

The fraction thus obtained is treated with yeast to remove sugars and then subjected to ion exchange column chromatography with an acidic or basic solvent (eg, 1N hydrochloric acid solution, 1N sodium hydroxide solution). It can be further purified by eluting.

The whitening cosmetic composition of the present invention comprises a concentrate of an extract or a squeezed liquid with a polar solvent for sluice and a conventional method for the base of various types such as emulsions, creams and lotions. It can be manufactured by mixing. At this time, it is more preferable to use the eluate obtained by the above-mentioned purification method, after appropriately neutralizing it, and lyophilize or vacuum-dry it by a conventional method. The amount of the concentrated extract of sardine extract or the squeezed liquid in these various preparations is not particularly limited, but is usually preferably about 0.1 to 20% by weight.

The whitening cosmetic composition of the present invention can also be produced by directly mixing the extract or squeezed liquid of the smoothing powder with a polar solvent with the base of each preparation.

[0011]

[Effect] Next, the melanin production inhibitory effect of the concentrate of the extract of Tsurumi shirasu contained in the whitening cosmetic composition of the present invention will be described with reference to test examples. Test example 1. Test substance (1) Concentrate of the purified liquid extract of Tsurureshii obtained in Production Example 1 (2) Arbutin (manufactured by Nacalai Tesque) 2. Bacteria used pJOE810 Mel, ⁺ E. Coli (E. coli that produces melanin by genetic engineering; Nuc
leic Acid Research Vol. 1
6, No. 17, 1988) 3. Use medium modified L-agar Bact tryptone (manufactured by Difco) 10 g sodium chloride (manufactured by Nacalai Tesque) 10 g L-tyrosine (manufactured by Nacalai Tesque) 400 mg copper sulfate (manufactured by Nacalai Tesque) 15 mg isopropyl β-D-thiogalactoside (manufactured by Sigma) 35 μg 3. Ampicillin 100 mg Bitek Agar (manufactured by Difco) 15 g Purified water 1000 ml 4. Test method After making modified L-agar, the medium was heated to 50 °
Incubate with pJOE810 Mel ⁺ , E. Add 1% of cryopreserved bacterial solution of E. coli to the medium and add 2 to the dish.
It dispensed 0 ml at a time. Dissolve the test substance in sterile purified water 1
A solution of 00 mg / kg was prepared and further diluted with sterile purified water to prepare a solution of 100 to 12.5 mg / ml. A bioassay cup (inner diameter: 6 mm, outer diameter: 8 mm, height: 10 mm) was placed in a modified L-agar, 250 μl of each diluted concentration solution of the test substance was injected, and the mixture was allowed to stand at room temperature for 1 hour for pre-diffusion. 37 ° C
After culturing for 24 hours, the diameter of the circle for inhibiting melanin production was measured with a caliper to 0.1 mm. 5. Test results

[Table 1] The extract of dried tsurugi contained in the whitening cosmetic composition of the present invention has a superior melanin production inhibitory effect as compared with arbutin.

Production Example 1 Tsurishui fruit (1000 g) was freeze-dried by a conventional method and then pulverized, followed by 80% methanol (1000 ml) at 80 ° C.
It was extracted for 3 hours while heating to room temperature. The residue (50 g) obtained by concentrating the obtained extract under reduced pressure was subjected to 10 to 100-fold column chromatography (HP-20, manufactured by Mitsubishi Kasei).
And eluate with water (1000 ml), and lyophilize the water eluate by a conventional method to obtain a dried extract of sardine (40 g).
Got

[0013]

EXAMPLES The whitening cosmetic composition of the present invention will be described below with reference to examples. Example 1 A liquid (800 ml) obtained by squeezing a succulent fruit (1000 g) is freeze-dried by a conventional method. The freeze-dried product and the fragrance were added to 99% ethanol and stirred, and then purified water was added and stirred to obtain a lotion agent having the following composition.

Example 2 Whole bean sprouts (1000 g) were placed in a beaker for 8
Add 0% methanol (1000 ml) and extract at 80 ° C. for 4 hours. The extract is concentrated under reduced pressure to give a residue (40 g). The concentrate of the extract of the soybean paste thus obtained was added to purified water together with glycerin, 1,3-butylene glycol and methyl paraoxybenzoate to obtain 70
Heat to ℃ to dissolve. On the other hand, liquid paraffin, cetanol, olive oil, sorbitan sesquioleic acid and polyoxyethylene oleyl ether (10 EO) are mixed and heated to 70 ° C. to dissolve. The water phase component and the oil phase component were mixed at 70 ° C., uniformly emulsified with a homomixer and cooled to obtain an emulsion having the following composition. Weight% Concentrate of Tsuruishi extract 5.0 Glycerin 5.0 1,3-Butylene glycol 5.0 Liquid paraffin 15.0 Cetanol 2.0 Olive oil 3.0 Sorbitan sesquioleate 1.0 Polyoxyethylene oleyl ether (10 EO) 2.0 Methyl paraoxybenzoate 0.2 Purified water 61.8

Example 3 A fruit (1000 g) of a sardine was freeze-dried by a conventional method and then pulverized. The crushed and dried fruit of the vinegar is extracted with 80% methanol (1000 ml) at 80 ° C. for 3 hours. The extract is dried under reduced pressure to obtain a residue (50 g). This residue was subjected to column chromatography (HP-2
0: Attached to Mitsubishi Kasei (500g), water (1000m)
The fraction obtained by elution in 1) is lyophilized by a conventional method.
The freeze-dried product of the water content of the smooth screen thus obtained is added to purified water together with propylene glycol and methyl paraoxybenzoate, and heated to 70 ° C. to dissolve. Stearyl alcohol, polyoxyethylene hydrogenated castor oil, glyceryl monostearate and propyl paraoxybenzoate are added to white petrolatum and heated to 70 ° C. to dissolve. The aqueous phase component and the oil phase component were mixed under heating (70 ° C.), stirred and cooled to obtain a cream preparation having the following composition. % By weight Freeze-dried product of water content of vinegar 1 White petrolatum 25 Stearyl alcohol 20 Propylene glycol 12 Polyoxyethylene hydrogenated castor oil 4 Glycerin monostearate 1 Methyl paraoxybenzoate 0.1 Propyl paraoxybenzoate 0.1 Purified water 36 .8

Claims (1)

[Claims] 1. A whitening cosmetic characterized in that it contains an extract or a squeezed liquid of a screen solvent of a polar solvent, or a concentrate thereof.