JPS5948808B2 - cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to cosmetics, and more particularly to highly safe cosmetics containing crude drug extracts with excellent skin whitening effects.

BACKGROUND OF THE INVENTION Among cosmetics, cosmetics containing medicinal agents that have a skin whitening effect are of great interest to consumers from the viewpoint of beauty, such as prevention of age spots and freckles.

Age spots and freckles are caused by the local production of large amounts of melanin pigment, which is synthesized in the body from tyrosine and dopa as starting materials through the action of enzymes and tyrosinase via dopaquinone and dopachrome. It is considered optimal to include a medicinal agent that inhibits tyrosinase activity.

On the other hand, in recent years, there has been an increase in skin disorders thought to be caused by medicinal agents incorporated into cosmetics, so it is necessary that the medicinal agents incorporated in cosmetics be even safer.

From this point of view, herbal medicines are natural products that have mild effects and have been used since ancient times, and can be said to be highly safe.Although the onset of effects is delayed, their effects are fully demonstrated with continuous use, and they are suitable for use in cosmetics. It is considered to be preferable as a medicinal agent to be incorporated.

In view of these circumstances, the present inventors have conducted various studies on herbal medicines that inhibit tyrosinase activity and exhibit excellent skin whitening effects.
It was discovered that extracts from Tsukizu, Seikatsu, Dokatsu, etc. have excellent skin whitening effects, and a patent application has already been filed for cosmetics containing these extracts (patent application filed in 1972).
No. 55313, Japanese Patent Application No. 52-3417).

Subsequently, the present inventors conducted further research and found that the extract of Agarious biaporous, a type of Western mushroom, is highly safe and has excellent skin benefits. The present invention was completed after discovering that the skin-whitening effect is exhibited, and that by mixing these with the extract, an even more excellent effect can be exhibited.

That is, the present invention provides a cosmetic product comprising extracts of one or more herbal medicines selected from Fufu, Hamabouji, and Western mushrooms, and optionally, a toki extract combined with these extracts. The cosmetics of the present invention exhibit excellent skin whitening effects without causing problems such as skin disorders.
Moreover, it provides a satisfying feeling of use.

Next, we will show the results of tests on the skin whitening effects of various herbal medicines.

The skin whitening effect can be expressed as the rate of inhibition of the action of tyrosinase, which produces melanin pigment from tyrosine.
The test was on the herbal medicine extract Tyrosinar-t! The test was carried out using 1g of total activity.

The crude drug was extracted by first heating and refluxing 100 tons of dried shredded crude drug powder with 300 rnl of 50% aqueous alcohol for 3 hours, and then filtering the mixture using a glass filter.

This treatment was performed twice, and then the F solution was combined and concentrated to dryness to obtain a crude drug extract.

Tyrosiner-h15g harmful activity 0 measurement was carried out as follows.

Sample solution: Appropriate amount of crude drug extract 1/1 syl
Dissolve in 10rnl of yate buffer (pH 6,8).

Enzyme solution: Dissolve 5 g of tyrosinase (2750 units/7v1 Sigma) in 10 g of distilled water.

Substrate solution: L-Dopa 30~ is dissolved by heating in 100 ml of 1/15M ΔDori buffer (pH 6,8).

Measurement procedure 7 Add 0.1rIll of enzyme solution to 1rnl of sample solution
and 0.9 ml of phosphate buffer (115M, pH 6,8) are added and incubated at 25°C for 10 minutes.

Then, 1 rnl of the substrate solution previously incubated at 25° C. is added to this reaction mixture and allowed to react for 5 minutes.

After the reaction, absorbance D1 at 475 mm is measured.

On the other hand, the absorbance D21 at 475 wnr/C# of a reaction solution similarly reacted using heat-inactivated enzyme
and the absorbance D3 in the case where no sample is added is measured, and the inhibition rate of tyrosinase activity is calculated from the following formula.

3-Dl Inhibition rate (correspondence) □ X100 3-D2 Inhibitory activity was compared based on 50% inhibition rate (ID, , ).

The results are shown in Table 1 below. As is clear from Table 1, Hamafu has an extremely excellent tyrosinase inhibitory activity, and Fufu and Western mushrooms also have sufficient tyrosinase-km harmful activity, although they are slightly lower than Toki, and a mixture of these or their equivalents can be used. It can be seen that by mixing the two, the effects are mutually exclusive and exhibit extremely excellent tyrosinase inhibitory activity.

Therefore, the cosmetic composition of the present invention contains the crude drug extract or the mixture thereof at 0.00%. O1 to 20.0% (by weight, the same applies hereinafter), preferably 1 to 10%.

Each herbal medicine extract, especially Hamafu, may be mixed alone, but as is clear from Table 1 above, it is possible to mix Fufu and Western mushrooms, or to mix them with Toki, or to mix Fufu or Western mushrooms. It is preferable to mix mushrooms and mushrooms,
Although the mixing ratio can be selected as appropriate, an equal situation mixture is usually preferred.

The crude drug extract to be used is, for example, an extract obtained by extracting a single item or several kinds of dried herbal powder powder several times with aqueous alcohol, filtering this extract, and concentrating to dryness. good.

In addition, this extract is further dissolved in distilled water and kept at a low temperature (e.g., 5°C or less) for several hours to several days (e.g., 96 hours).
After dialysis, the extra-dialysis fluid is collected and concentrated, or this can be further processed with Sephadex such as Sephadex G-15, DEAE-Sephadex, home lullose, Sepharose, Bio-gel, or ion exchange. On columns such as resin.

It may also be a purified extract, such as one obtained by subjecting it to chromatography using a solvent such as ion-exchanged water or a buffer solution (for example, a phosphate buffer solution with a pH of 4 to 8), and concentrating the active fraction.

The cosmetics of the present invention include bags, lotions, creams, emulsions,
It can be in conventional forms such as cleansers and soaps, each of which is manufactured according to conventional methods.

The following Table 2 shows the cream C (invention cream) of Example 5 described below containing 1% Hamabofu extract, and the cream (control cream) in which the Hamabofu extract was removed from the composition of the cream.
The results of a test on the skin whitening effect of 50 statistically equivalent women when used continuously for 3 months are shown.

As shown in Table 2, the cream of the present invention is more effective in improving dark spots, age spots, and freckles and in restoring skin from sunburn than the control cream.

In addition, using the milk lotion of Example 7 described below (product of the present invention) containing 1% of windproof, Western mushroom B and Toki mixed extract, and the milk lotion in which the crude drug extract was removed from the composition of the milk lotion, statistics were conducted. Table 3 below shows the results of a test conducted in the same manner as in Table 2 above on 100 women who were physically equivalent.

As shown in Table 3, the milk lotion of the present invention is more effective in improving dark skin, age spots, and freckles and restoring sunburned skin than the control milk lotion.

Next, the present invention will be explained in more detail with reference to Examples, but the present invention is not limited thereto.

Example 1 Lotion (oil-based) Sorbitol (70%) 3. (1
Guri De Rin 5.0
y Hamakaze Extract 1. O? water
70. (1) Mix and dissolve these components.

Add allantoin 0.
12 Polyoxyethylene hydrogenated castor oil derivative 0.5 y
Ethanol 20. Add an appropriate amount of the mixed solution while stirring to make a homogeneous solution, and obtain a lotion having a skin whitening effect.

Example 2 Lotion (drying) Ethylene glycol 2.5g Propylene glycol 5.0? Silicone 0.00011 Western mushroom extract 0.12 Water
74.9S' Mix and dissolve these ingredients.

To this, urea 1.
(1 Polyoxyethylene sorbitan monolaurate 1.22 Ethanol 15. OS'ε-
Aminocaproic acid 0.12 fragrance
Add an appropriate amount of the mixed solution while stirring to obtain a homogeneous solution to obtain a lotion having a skin whitening effect.

Example 3 Facial cleansing cream glycerin 2. O
t stearic acid 20. (H'
Myristic acid 10. (1
Lauric acid 5, Ot polyoxyethylene lauryl ether 1.0? Windproof extract 10.05'Fragrance S and preservative Appropriate amounts of these ingredients were mixed and dissolved by heating at about 75°C, and this was heated to about 75°C.Potassium hydroxide 5.5I water
After adding 46.5 g of the solution with stirring, it is cooled to obtain a facial cleansing cream with skin whitening effect.

Example 4 Back polyvinyl alcohol 15. (1 preservative 0.1r water
75.1' Windproof Extract 0.5v Mix these ingredients, heat and dissolve at about 80°C, then cool to room temperature. Glycerin 4-Of Ethanol 5.02 Fragrance
Mix appropriate amounts and dissolve uniformly to obtain a pack.

Example 5 Oil-based cream beeswax 10. Of paraffin wax 6.0g lanolin 3.0g isopropyl myristate 6.0g squalane
8.0g liquid paraffin
25.0. @ Hamakaze Extract
1.0g vitamin E attate
0.2g polyoxyethylene sorbitan monostearate 1.8g sorbitan monostearate 4.2g preservative
Prepylene glycol 2.0? Mix appropriate amounts of these ingredients, heat and dissolve at about 74°C, and then heat to about 75°C. Borax 0.72 glycyrrhizin 0.2 urea
5.0? water
Add the mixture of 26.6r with stirring, and after cooling, add an appropriate amount of fragrance at 55°C, cool to 45°C while stirring, and leave to obtain an oily cream.

Example 6 Soap beef tallow 40.0? Ya tank
10.0” castor oil
4,5t36°Be sodium hydroxide aqueous solution 45. Or these ingredients are heated and mixed, cooled to 45℃, and added to this, Windproof, Toki Equivalent Mixed Extract 0.05F Fragrance
Add appropriate amount, stir, and leave to obtain soap.

Example 7 Milk Lotion Atearic Acid 2.02 Tanol 0.51 Lanolin 2.02 Isopropyl Myristate 2.0? Squalane
3. Of liquid paraffin
8.01 Porioxyethylene cetyl ether 1.71 Sorbitan monostearate
0.82 Windproof, Western mushroom, Toki equivalent mixed extract 15.0 Vitamin A acetate) 0.2f Mix these ingredients, heat and dissolve at about 75°C, add about 7
Triethanolamine heated to 5℃ 1.0? Glycerin 4.0? water
59.5S' Flavor and preservative Add appropriate amounts, stir and cool to obtain milk lotion.

Claims (1)

[Claims] 1. Windbreak, Hamakazegyo Bikkkuritake (Agaricua)
-bisporous) or two selected from
A cosmetic product that is characterized by containing extracts of more than one species of herbal medicine. 2. The cosmetic according to item 1 above, wherein the herbal medicine is Hamafu. 3. The cosmetic according to item 1 above, wherein the crude drug is windproof. 4. The cosmetic according to item 1 above, which is made from the crude drug. 5. The above-mentioned No. 1, wherein the herbal medicine is a mixture of windbreak and tsukuritake.
Cosmetics. 6. Said first compound containing 0.01 to 20% of the extract by weight.
Cosmetics according to any one of Items 1 to 5. I. The cosmetic composition according to item 6 above, which contains 1 to 10% by weight of the extract. 8 Windproof, Hamakafu S, Surprise Take (Agaricus)
-bisporous) or two selected from
A cosmetic product characterized by containing an extract of more than one species of herbal medicine and an extract of Oriental chinensis. 9 A total of 0.01 to 2 of the herbal medicine extract and herbal extract
The cosmetic according to item 8 above, which is formulated in a weight ratio of 0.