JP2009209063A - Skin preparation for external use, or skin cosmetic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin preparation for external use or a skin cosmetic containing an extracted liquid or extract essence obtained by extracting the fleshy stems of Cistanche tubulosa with water, a lower aliphatic alcohol or its hydrate, as an active ingredient. <P>SOLUTION: This skin preparation for external use or skin cosmetic is provided by extracting the fleshy stems of the C. tubulosa with water, the lower aliphatic alcohol or its hydrate, confirming the effectiveness of the extracted liquid or extract essence for the skin, and incorporating the extracted liquid or extract essence, or a specific compound contained in them as an active ingredient. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a skin external preparation or skin containing, as an active ingredient, an extract or an extract obtained by extracting meat stalks of the antarctic parasitoid kankanikuyuyo with water or a lower aliphatic alcohol or a hydrated product thereof. The present invention relates to a cosmetic and a skin external preparation or skin cosmetic containing a specific compound contained in the extract or extract as an active ingredient.

  Okanbanceae plant, Kankaniku Juyo (scientific name: Cistanche tubulosa (Shrenk) R. Wight] is a parasitic plant that grows naturally in northern Africa, the Arabian region, and regions from West Asia to Pakistan and India, and parasitizes the roots of Salvadora or Calotropis genus plants. The decoction is used for the treatment of diarrhea and swelling in Pakistan, and the fleshy stem is used for the treatment of infertility and Alzheimer's disease in China. For example, it is disclosed in Non-Patent Document 1 and Non-Patent Document 2. Has been.

  The tonicity or tonic agent obtained from an extract from citrus meat stalk, glycoside compound and their use were previously disclosed in the patent literature by the present inventors (Patent Literature 1).

However, no research has been conducted on skin external preparations and skin cosmetics containing an extract or extract from citrus carcass.
JP 2007-191416 A Kobayashi H. et al. , Et. al. , Chem. Pharm. Bull. , 35, 3309-3314 (1987) Xinjiang Chugoku National Institute of Ethnic Medicine "Shinpotsu Cultivation Technology for Common Use", Xinjiang Science and Technology Publishers, 84-88 (2004)

  The present invention relates to an extract or extract extracted from the fleshy stalk of kankanikuyu which has been conventionally used for the treatment or improvement of the above-mentioned symptoms and can be used not only for medicinal purposes but also for food. It is an object to provide a skin external preparation or a skin cosmetic used. The present invention further provides use of a specific compound contained in the extract or extract as a skin external preparation or skin cosmetic.

  As a result of diligent research on an extract or extract from citrus carcass, the present inventor has conducted a melanin production inhibition test using B16 melanoma as an index of activity evaluation as a topical skin preparation or skin cosmetic. And found that these have activity, and found applicability as a whitening agent. Furthermore, as a result of conducting an in vitro antioxidant test, an activity comparable to that of ascorbic acid and tocopherol was found, and an applicability as an anti-aging / anti-wrinkle agent was found.

  Furthermore, it was confirmed that the active ingredient permeated through the stratum corneum and distributed to the epidermis layer and the dermis layer, which are living cells, in a percutaneous absorption test using pork skin. It has been found that it can be applied to an external preparation for skin or a skin cosmetic that is expected to be applied to the skin. The present invention has been completed based on these findings.

  That is, the present invention includes a skin external preparation characterized by containing, as an active ingredient, an extract or extract obtained by extracting meat stalks of kankanikusuyo with water, a lower aliphatic alcohol or a hydrate thereof. A skin cosmetic is provided (claim 1).

  The extract or extract contains a compound named etinacoside, acteoside, cancanoside F, cancanoside G, cancanoside H, cancanoside A, cancanoside B, cancanoside C, cancanoside D, cancanol, cancanoside E. They exhibit activity as whitening agents or antioxidants. Therefore, the present invention provides the external preparation or cosmetic according to claim 1, wherein etinacoside, acteoside, cancanoside F, cancanoside G, cancanoside H contained in an extract or extract from citrus carcass. A skin external preparation or a skin cosmetic comprising, as an active ingredient, at least one selected from: Cancanoside A, Cancanoside B, Cancanoside C, Cancanoside D, Cancanol, and Cancanoside E (Claim 2).

  And the content of the extract or extract from the fleshy stalk of kankanikuyu in skin external preparations or skin cosmetics, or etinacoside, acteoside, kancanoside F, kancanoside G contained in the extract or extract, The content of cancanoside H, cancanoside A, cancanoside B, cancanoside C, cancanoside D, cancanol and cancanoside E varies slightly depending on the form and use of the topical skin preparation or skin cosmetic, but it is 0.001 mass as a solid content. % To 20% by mass is preferable (Claim 3).

  As an active ingredient, an extract or extract obtained by extracting the meat stalk of kankanikusuyo with water or a lower aliphatic alcohol or its hydrate, or a compound contained in the extract or extract of the present invention. The skin external preparation or skin cosmetic to be contained exhibits excellent whitening and antioxidant effects. Therefore, human or animal skin external preparations or skin cosmetics containing them exhibit excellent whitening and antioxidant effects.

  The topical skin preparation or skin cosmetic of the present invention is an extract or extract obtained by extracting a carcass of citrus carp with water, a lower aliphatic alcohol or a hydrate thereof, or in the extract or extract And ethanocoside, acteoside, cancanoside F, cancanoside G, cancanoside H, cancanoside A, cancanoside B, cancanoside C, cancanoside D, cancanoside and cancanoside E contained as active ingredients.

  The extract or extract of citrus carcass used in the external preparation for skin or skin cosmetic of the present invention can be obtained, for example, by the method described in Patent Document 1. Specifically, it can be obtained by extracting the citrus meat stalk as it is or from the one obtained by changing the shape by crushing, crushing, cutting, grinding or the like, or by drying as follows. From the viewpoint of extraction efficiency, it is preferable to use a dried product of edible kankanikuyu, which has undergone shape change by pulverization, crushing, cutting, grinding or the like.

  Examples of the extraction solvent used for obtaining an extract from citrus carcass include lower alcohols having 1 to 4 carbon atoms, specifically, methanol, ethanol, 1-propanol, 2-propanol. 1-butanol, 2-butanol, 2-methyl-1-propanol, 2-methyl-2-propanol or a mixture thereof, or a hydrous alcohol containing up to 30% by volume of water. Of these, methanol or ethanol is preferred. These extraction solvents are used in an amount of about 1 to 50 times (volume), preferably about 10 to 30 times the extraction material.

  The extraction temperature can be arbitrarily set between room temperature and the boiling point of the solvent. For example, the extraction material is mixed with the extraction solvent at a temperature of 50 ° C. to the boiling point of the extraction solvent under shaking or non-shaking or refluxing. It is appropriate to immerse in the solution. When the extraction material is immersed under shaking, it is appropriate to carry out for about 30 minutes to 10 hours, and when it is immersed under non-shaking, it is appropriate to carry out for about 1 hour to 20 days. Moreover, when extracting under reflux of an extraction solvent, it is preferable to heat to reflux for 30 minutes to several hours. In addition, although it is possible to extract by immersing at a temperature lower than 50 ° C., it is preferable to immerse for a longer time than the above time. The extraction operation may be performed only once for the same material, but is preferably repeated a plurality of times, for example, about 2 to 5 times.

  In the extract obtained by the above extraction step, kankanikujuyo-containing components are eluted. The extract thus obtained may be added as it is to the skin external preparation or skin cosmetic of the present invention. Moreover, you may concentrate the said extract and add as an extract. Concentration is preferably carried out at a low temperature and under reduced pressure. This concentration may be carried out until dry. The filtrate may be concentrated by filtration before concentration. The extract may be in a concentrated state, or may be in the form of powder or lyophilized product. Methods known in the art can be used for the method of concentrating, powdered and freeze-dried.

  The obtained extract may be subjected to purification treatment before and after concentration. As the purification treatment, a chromatographic method, an elution method using an ion exchange resin, partition extraction with a solvent, or the like can be employed alone or in combination. For example, examples of the chromatographic method include normal phase chromatography, reverse phase chromatography, thin layer chromatography, centrifugal liquid chromatography, high performance liquid chromatography, and the like or a combination thereof. In this case, purification conditions such as a carrier and an elution solvent can be appropriately selected according to various chromatographies.

  The extract or extract contains at least one of the compounds represented by the following formulas (1) to (11). That is,

Echinacoside represented by the formula (1),

An isoacteoside represented by formula (2),

Cancanoside F represented by the formula (3),

Cancanoside G represented by formula (4),

Represented by the formula (5) is kankaside-H,

Formula (6) represents cancanoside A (kankanside-A),

In the formula (7), cancanoside B (kankanside-B),

Represented by the formula (8) is cancanoside-C,

In the formula (9), cancanoside D (kankanside-D),

Kankanol represented by formula (10),

It is cancanoside E (kankanside-E) represented by Formula (11).

  The activity of an extract or extract from citrus meat stalk was found in a melanin production inhibition test and an antioxidant test conducted as an index for evaluating the activity as a topical skin preparation or skin cosmetic. Therefore, each compound represented by the above formulas (1) to (11) and an extract or extract of kankanikujuo containing each compound can be used as a skin external preparation or a skin cosmetic. The topical skin preparation or skin cosmetic described in the present invention includes application as a functional cosmetic in addition to pharmaceuticals.

  The above extract or extract extract and the compounds of the above formulas (1) to (11) are diluted as they are or with an appropriate medium, respectively, to obtain a skin external preparation or skin. It can be used by a known method in the field of cosmetic production. Moreover, the skin external preparation or skin cosmetic of the present invention comprises the above-mentioned extract or extract of kankanikuyu and the compounds of the above formulas (1) to (11) in a normal skin external preparation or skin cosmetic. It can also be manufactured.

  The content of the citrus extract or extract or the compounds of the above formulas (1) to (11) in the external preparation for skin or skin cosmetic of the present invention is the skin external preparation or skin makeup as a solid content. It is 0.001-20 mass% in the total amount of a material, Preferably it is 0.01-10 mass%. If the content is less than the above range, the melanin production inhibitory effect and the antioxidant effect may not be sufficiently exhibited. On the other hand, even if the content exceeds the above range, a significant improvement in the effect commensurate with the content is not recognized.

  The topical skin preparation or skin cosmetic of the present invention is an extract or extract obtained by extracting a meat stalk of kankanikusuyo with water, a lower aliphatic alcohol or a hydrate thereof, or the extract or Although it is comprised by containing the compound of said Formula (1)-(11) contained in an extract, besides this essential component, it is normally used for skin external preparations and skin cosmetics, such as cosmetics and a pharmaceutical. Other components can be appropriately blended. Examples of such components include powder components, liquid fats and oils, solid fats and oils, waxes, hydrocarbons, higher fatty acids, higher alcohols, esters, silicones, anionic surfactants, cationic surfactants, amphoteric surfactants, non-surfactants. Ionic surfactants, moisturizers, water-soluble polymer compounds, thickeners, film forming agents, UV absorbers, sequestering agents, lower alcohols, polyhydric alcohols, sugars, amino acids, animals and plants or microorganisms Hydrolyzed proteins and their derivatives, organic amines, synthetic resin emulsions, pH adjusters, skin nutrients, vitamins, antioxidants, antioxidant aids, fragrances, water and the like. In addition, other whitening agents such as vitamin C, magnesium ascorbate phosphate, glucoside ascorbate, arbutin, and kojic acid can be appropriately blended. In addition to these, other components can be appropriately added as long as the properties of the external preparation for skin and skin cosmetics of the present invention are not impaired.

  The skin external preparation or skin cosmetic of the present invention is widely applicable in the fields of cosmetics, pharmaceuticals, and quasi drugs. The dosage form is not particularly limited as long as it can be applied to the skin. For example, it is a solution, emulsion, solid, semi-solid, powder, powder dispersion, water-oil two-layer separation, water-oil. -Arbitrary dosage forms such as powder three-layer separation, ointment, gel, aerosol, mousse, stick, etc. are applied. Moreover, the usage form is also arbitrary, for example, facial cosmetics, such as lotion, emulsion, cream, pack, essence, and gel, and makeup cosmetics such as foundation, makeup base, and concealer.

  The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples. In addition, content etc. are shown in mass% unless otherwise specified. In addition, prior to the examples, production examples of the citrus extract used in the examples are shown.

Production example of kankanikuyuyo extract 5 kg of dried kankanikuyujo meat stalk was pulverized, and about 50 l of methanol was added thereto, followed by extraction by heating under reflux for 3 hours. After extraction, the extraction residue was separated by filtration, and the residue was extracted twice by the same operation again. The extracted filtrates were combined, concentrated to dryness under reduced pressure, and pulverized to obtain a kankanikujuyo extract. This extract was confirmed to contain compounds (1) to (11) and acteoside by the chromatography method described in the Examples of Patent Document 1. In the following Examples 1 to 3 and 5 to 16 (other than Example 4), this extract was dissolved in a 30% aqueous ethanol solution (w / w) to a concentration of 5% by mass of the total amount and used. Hereinafter, this solution (solid content: 5%) is referred to as Kankan extract. In Example 4, the powdered citrus extract was dissolved in a 30% aqueous ethanol solution and used.

  Hereinafter, the effectiveness of the kanka extract on the tyrosinase activity inhibitory effect, melanin production inhibitory effect, and transdermal absorbability effect will be described with reference to data.

Example 1. Tyrosinase activity inhibitory action The tyrosinase activity inhibitory action of Kanka extract was examined by the following test method. For comparison, kojic acid, which is considered to have a strong tyrosinase activity inhibitory effect, was used.

[Tyrosinase activity inhibition test]
1 mg of mushroom-derived tyrosinase (manufactured by SIGMA) was weighed and dissolved in 1 ml of a 50 mmol / l phosphate buffer (pH 6.8) as an enzyme solution. 100 μl of a 2 mmol / l tyrosine aqueous solution, 50 mmol / l phosphate buffer (pH 6.8), and a DMSO (dimethyl sulfoxide) solution of the test substance have a final concentration of 0.1% (v / v), 40 μl of mushroom-derived tyrosinase enzyme solution was added, and the total amount was adjusted to 200 μl with 50 mmol / l phosphate buffer (pH 6.8). The reaction was carried out in a constant temperature bath at 25 ° C., and the absorbance at 490 nm was measured after 30 minutes. The tyrosinase activity inhibition rate was calculated according to the following equation. The larger the tyrosinase activity inhibition rate, the higher the tyrosinase activity inhibition rate.

  In the above formula, A is the absorbance when an enzyme is added without adding a test substance, B is the absorbance when neither a test substance nor an enzyme is added, C is the absorbance when a test substance and an enzyme are added, and D is the test substance. Represents the absorbance when no enzyme is added.

  Table 1 shows the inhibition rate of tyrosinase activity of the extract of kanka and kojic acid used for comparison, and the inhibition rate is an average value ± standard error of four measurement results.

  As shown in Table 1, the kanka extract is less effective than kojic acid, which has been reported to have a tyrosinase activity inhibitory effect, but has a tyrosinase activity inhibitory action. It is.

Example 2 Melanin production inhibitory effect The following test method was used to examine the melanin production inhibitory effect of the kankanikuyuyo extract. For comparison, arbutin, which is considered to have a melanin production inhibitory effect, was used.

[Melanin production inhibition test]
Mouse B16 melanoma 4A5 cells were seeded at 20,000 cells / well in a 48-well plate. On the next day, a culture medium containing 0.2 mmol / l theophylline and a DMSO (dimethyl sulfoxide) solution of the test substance so that the concentration of the DMSO solution is 0.1% (v / v) with respect to the whole medium. Was replaced. Three days after the addition of the test substance, the protein amount of melanoma cells was quantified using BCA Protein assay kit (manufactured by PIERE), and the melanin production amount was determined by alkali solubilization method (after disrupting the cells, the final concentration was 2 mol). After adding the sodium hydroxide so that it becomes / l and heating at 60 ° C. for 15 minutes to solubilize melanin, the absorbance at 450 nm was measured using a microplate reader. The amount of melanin was calculated from a calibration curve prepared using synthetic melanin as a standard product, and the amount of melanin per unit protein was calculated by dividing the amount of melanin by the amount of protein. Wells in which theophylline and the test substance were not added to the medium were used as the normal group, and wells to which theophylline was added but the test substance was not added were used as the control group. The melanin production inhibition rate was calculated from the following equation.

  In the above formula, E is the amount of melanin per unit protein (g / g) at the time of sample addition, F is the amount of melanin per unit protein in the normal group (g / g), and G is the amount per unit of the control group The amount of melanin (g / g) is shown.

  Table 2 shows the melanin production inhibition rate of the kanka extract and arbutin used for comparison. The melanin production inhibition rate is an average value ± standard error of four tests.

  As is clear from Table 2, the kanka extract showed almost the same melanin production inhibitory effect as arbutin used for comparison.

Example 3 FIG. Antioxidant effect The antioxidant effect of ethinacoside and acteoside, which are the main constituent compounds contained in the extract of Kanka, was confirmed. For comparison, ascorbic acid and α-tocopherol were used. In addition, the following antioxidant effect is represented by the relative value compared with control. For control, a 25 mmol / l phosphate buffer solution having a pH of 7.0 and no added radical generator was used.

[Antioxidant effect confirmation test]
Antioxidant effect measurement measured the effect on peroxy radicals and hydroxy radicals with different polarities. For measurement of the antioxidant effect on water-soluble peroxy radicals, AAPH [2,2′-azobis (2-amidopropane) dihydrochloride] was used as a radical generator azo compound dissolved in a phosphate buffer. For the measurement of the antioxidant effect on the fat-soluble peroxy radical, 1 mmol / l AMVN [2,2′-azobis (2,4-dimethylvaleronitile)] was dissolved in a phosphate buffer and used. Hydroxyl radicals were generated by a Fenton reaction using cobalt and hydrogen peroxide (a reaction in which one molecule of hydrogen peroxide is a divalent metal ion and one molecule of hydroxide ion and one molecule of hydroxyl radical is generated). After adding various radical generating reagents in the phosphate buffer, a test substance was added and heated at 37 ° C. Then, the luminol reagent was mixed and the chemiluminescence intensity was measured using a photon counter. Relative chemiluminescence of luminol control, the halved value calculated as IC _50.

Table 3 shows the antioxidant effect results (IC ₅₀ values) of etinacoside, acteoside and ascorbic acid and α-tocophenol used for comparison. Since ascorbic acid is water-soluble, fat-soluble peroxy radicals cannot be measured, and since α-tocopherol is fat-soluble, water-soluble peroxy radicals and hydroxy radicals cannot be measured. .

  As is apparent from Table 3, echinacoside and acteoside, which are the main constituent compounds of kanka extract, are about 1.6 times ascorbic acid in water-soluble peroxide radicals, about 30 to 45 times in alpha-tocopherol in fat-soluble peroxide radicals, and hydroxy. Radicals were found to have four times the antioxidant effect of ascorbic acid.

Example 4 Transdermal absorbability test Using the skin of the back of a miniature pig (Yucatan Micropig Skin Set: manufactured by Charles River), the transdermal absorbability of Kankan extract was confirmed by the following test method.

[Transdermal absorption test]
The stratum corneum was cut into 2 × 2 cm by filling the receptor tube of Franz-type percutaneous absorption tube with an inner diameter of 11 mm with 0.1 mol / l isotonic phosphate buffer (pH 7.2), removing the subcutaneous fat thereon. Then, the skin of minipig with epidermis and dermis was placed, and the donor tube was placed and fixed with a clamp so that the center line of the receptor tube was the same. The sample was percutaneously absorbed by applying 0.3 ml of the percutaneously absorbable sample to the donor tube while keeping the receptor tube at 37 ° C. while stirring the solution in the tube at 600 rpm with a stir bar. Went for hours. The amount of kanka extract absorbed percutaneously is determined by thoroughly washing the sample remaining in the donor tube with water, separating the stratum corneum + epidermis and dermis, immersing each in 10 ml of 30% ethanol aqueous solution and crushing with a homogenizer. After extraction and membrane filtration, liquid chromatography was performed under the following conditions. In this test, acteoside and etinacoside, which are components contained in a large amount of kanka extract, were used as an index for percutaneous absorption of kanka extract.

Liquid chromatography analysis condition column: Cosmocil 5C18-MS-II (4.6 mm ID × 150 mm)
Mobile phase: A liquid; 0.1% acetic acid aqueous solution, B liquid; acetonitrile Gradient condition: B liquid concentration 0-2 min; 5%
2-15min: 5% → 80%
15-19min: 100%
Flow rate: 1 ml / min
Column temperature: 40 ° C
Measurement wavelength: 254 nm

  For the transdermally absorbable sample, a solution obtained by dissolving a powdered citrus extract into a 30% ethanol aqueous solution (w / w) to a concentration (w / w) of 1%, 5%, 10% Using. In addition, the powdered citrus extract used contained 9.1% by mass of acteoside and 25.5% by mass of etinacoside. Table 4 shows penetration amounts (μg) of acteoside and etinacoside that have penetrated into the epidermis (including the stratum corneum) and dermis.

  As shown in Table 4, when the applied concentration exceeds 5%, the absorption of the kanka extract becomes rate limiting, so there is no difference in the amount of absorption. However, at a concentration lower than that, the higher the concentration of the applied solution, the higher the concentration. It can be seen that the percutaneous absorption amount of the extract of Kanka increases. For this reason, when applied at a concentration of 5% or more, it is clear that the extract of kanka stays in the epidermis and dermis for a long time, and is considered to exert a whitening effect.

  In the following, prescriptions and production methods for external preparations for skin and skin cosmetics using kanka extract are shown, but the present invention is not limited to these.

Example 5
A skin cream was prepared according to the following formulation and preparation method.
(Prescription)
Ingredient Mass%
Stearic acid 5.0
Stearyl alcohol 4.0
Isopropyl myristate 18.0
Glycerol monostearate 3.0
Propylene glycol 10.0
Kanka extract 0.1
Potassium hydroxide 0.2
Sodium bisulfite 0.05
Preservatives Appropriate amount of perfume Appropriate amount of ion-exchanged water 100
Propylene glycol and potassium hydroxide were added to ion-exchanged water and dissolved, and the mixture was heated and maintained at 70 ° C. (aqueous phase). The other components were mixed, heated and melted and kept at 70 ° C. (oil phase). The oil phase was gradually added to the aqueous phase, and after the addition was completed, the reaction was maintained at that temperature for a while, then emulsified uniformly with a homomixer, and cooled to 30 ° C. while stirring well to prepare a skin cream. .

Example 6
A skin cream was prepared according to the following formulation and preparation method.
(Prescription)
Ingredient Mass%
Stearic acid 5.0
Sorbitan monostearate ester 2.5
Polyoxyethylene (20) sorbitan monostearate 1.5
Arbutin 7.0
Sodium bisulfite 0.03
Propylene glycol 10.0
Kanka extract 0.05
Glycerin trioctanoate 10.0
Squalene 5.0
Octyl paradimethylaminobenzoate 3.0
Ethylenediaminetetraacetic acid disodium salt 0.01
Ethylparaben 0.3
Perfume Appropriate amount of ion-exchanged water Amount to be 100 in total (production method)
Ion exchanged water was dissolved by adding propylene glycol and ethylenediaminetetraacetic acid disodium salt and heated to 70 ° C. (aqueous phase). The other components were mixed, dissolved by heating and kept at 70 ° C. (oil phase). The oil phase was gradually added to the aqueous phase, pre-emulsified at 70 ° C., uniformly emulsified with a homomixer, and then cooled to 30 ° C. while stirring well to prepare a skin cream.

Example 7
A skin cream was prepared according to the following formulation and preparation method.
(Prescription)
Ingredient Mass%
Solid paraffin 5.0
Beeswax 10.0
Vaseline 15.0
Liquid paraffin 41.0
Glycerin monostearate ester 2.0
Polyoxyethylene (20) sorbitan monolaurate 2.0
Soap powder 0.1
Borax 0.2
Kanka extract 0.1
Sodium bisulfite 0.03
Ethylparaben 0.3
Perfume Appropriate amount of ion-exchanged water Amount to be 100 in total (production method)
Soap powder and borax were added to ion exchange water, dissolved by heating and kept at 70 ° C. (water phase). The other components were mixed, heated and melted and kept at 70 ° C. (oil phase). The oil phase was gradually added to the aqueous phase while stirring. After the addition, the mixture was uniformly emulsified with a homomixer. After emulsification, the mixture was cooled to 30 ° C. while stirring well to prepare a skin cream.

Example 8
An emulsion was prepared according to the following formulation and manufacturing method.
(Prescription)
Ingredient Mass%
Stearic acid 2.5
Cetyl alcohol 1.5
Vaseline 5.0
Liquid paraffin 10.0
Polyoxyethylene (10) monooleate 2.0
Polyethylene glycol 1500 3.0
Triethanolamine 1.0
Carboxyvinyl polymer 0.05
Kanka extract 0.01
Sodium bisulfite 0.01
Ethylparaben 0.3
Perfume Appropriate amount of ion-exchanged water Amount to be 100 in total (production method)
Carboxyvinyl polymer was dissolved in a small amount of ion-exchanged water (A phase). Polyethylene glycol 1500 and triethanolamine were added to the remaining ion-exchanged water, dissolved by heating and kept at 70 ° C. (aqueous phase). The other ingredients were mixed, heated and melted, and kept at 70 ° C. (oil phase). An oil phase was added to the aqueous phase, preliminarily emulsified, and the A phase was added, and the mixture was uniformly emulsified with a homomixer.

Example 9
An emulsion was prepared according to the following formulation and manufacturing method.
(Prescription)
Ingredient Mass%
Microcrystalline wax 1.0
Beeswax 2.0
Lanolin 20.0
Liquid paraffin 10.0
Squalane 5.0
Sorbitan sesquioleate ester 4.0
Polyoxyethylene (20) sorbitan monooleate 1.0
Propylene glycol 7.0
Kanka extract 1.0
Sodium bisulfite 0.01
Ethylparaben 0.3
Perfume Appropriate amount of ion-exchanged water Amount to be 100 in total (production method)
Propylene glycol was added to ion-exchanged water and heated to maintain 70 ° C. (aqueous phase). The other components were mixed, heated and melted and kept at 70 ° C. (oil phase). While stirring the oil phase, the aqueous phase was gradually added thereto, and the mixture was uniformly emulsified with a homomixer. After emulsification, the mixture was cooled to 30 ° C. while stirring well to prepare an emulsion.

Example 10
A jelly-form skin external preparation was prepared according to the following formulation and production method.
(Prescription)
Ingredient Mass%
Ethanol (95%) 10.0
Dipropylene glycol 15.0
Polyoxyethylene (50) oleyl ether 2.0
Carboxyvinyl polymer 1.0
Sodium hydroxide 0.15
L-Arginine 0.1
Kanka extract 5.0
Sodium 2-hydroxy-4-methoxybenzophenone sulfonate 0.05
Ethylenediaminetetraacetate, trisodium, dihydrate 0.05
Methylparaben 0.2
Perfume Appropriate amount of ion-exchanged water Amount to be 100 in total (production method)
The carboxyvinyl polymer was uniformly dissolved in ion-exchanged water, while the kankanikujou extract and polyoxyethylene (50) oleyl ether were dissolved in 95% ethanol and added to the aqueous phase. Next, after adding other components, the mixture was neutralized with sodium hydroxide and L-arginine to increase the viscosity, thereby preparing a jelly-like skin external preparation.

Example 11
A cosmetic solution was prepared according to the following formulation and manufacturing method.
(Prescription)
Ingredient Mass%
[Phase A]
Ethyl alcohol (95%) 10.0
Polyoxyethylene (20) octyldodecanol 1.0
Pantotenyl ethyl ether 0.1
Kanka extract 2.0
Methylparaben 0.15
[Phase B]
Potassium hydroxide 0.1
[Phase C]
Glycerin 5.0
Dipropylene glycol 10.0
Sodium bisulfite 0.03
Carboxyvinyl polymer 0.2
Purified water Amount that makes 100 in total (production method)
The A phase and the C phase were uniformly dissolved, and the A phase was added to the C phase and solubilized. Subsequently, the phase B was added and then filled to prepare a cosmetic liquid.

Example 12
A pack was prepared according to the following formulation and manufacturing method.
(Prescription)
Ingredient Mass%
[Phase A]
Dipropylene glycol 5.0
Polyoxyethylene (60) hydrogenated castor oil 5.0
[Phase B]
Kanka extract 0.05
Olive oil 5.0
Tocopherol acetate 0.2
Ethylparaben 0.2
Fragrance 0.2
[Phase C]
Sodium bisulfite 0.03
Polyvinyl alcohol (degree of saponification 90, degree of polymerization 2,000) 13.0
Ethanol 7.0
Purified water Amount that makes 100 in total (production method)
A phase, B phase, and C phase were uniformly dissolved, and B phase was added to A phase to solubilize. Next, this was added to Phase C and then filled to prepare a pack agent.

Example 13
A solid foundation was prepared according to the following formulation and manufacturing method.
(Prescription)
Ingredient Mass%
Talc 43.1
Kaolin 15.0
Sericite 10.0
Zinc flower 7.0
Titanium dioxide 3.8
Yellow iron oxide 2.9
Black iron oxide 0.2
Squalane 8.0
Isostearic acid 4.0
Polyoxyethylene sorbitan monooleate 3.0
Isocetyl octoate 2.0
Kanka extract 0.5
Preservative Appropriate amount Fragrance Appropriate amount (Manufacturing method)
Thoroughly blend powder components of talc, kaolin, sericite, zinc white, titanium dioxide, yellow iron oxide and black iron oxide with a blender, and add oil to squalane, isostearic acid, polyoxyethylene sorbitan monooleate and isocetyl octanoate. Ingredients, kankanikujuyo extract, preservative, and fragrance were added and kneaded well, then filled into a container and molded to prepare a solid foundation.

Example 14
A cream type emulsified foundation was prepared according to the following formulation and production method.
(Prescription)
Ingredient Mass%
[Powder part]
Titanium dioxide 10.3
Sericite 5.4
Kaolin 3.0
Yellow iron oxide 0.8
Bengala 0.3
Black iron oxide 0.2
[Oil phase]
Decamethylcyclopentasiloxane 11.5
Liquid paraffin 4.5
Polyoxyethylene-modified dimethylpolysiloxane 4.0
[Water phase]
Kanka extract 0.5
Purified water 50.0
1,3-butylene glycol 4.5
Sorbitan sesquioleate 3.0
Preservative Appropriate amount Fragrance Appropriate amount (Manufacturing method)
After the aqueous phase was heated and stirred, the powder part sufficiently mixed and ground was added and homomixed. Furthermore, after adding the heat-mixed oil phase and carrying out the homomixer process, the fragrance | flavor was added with stirring, it cooled to room temperature, and the cream type emulsified foundation was prepared.

Example 15
A lotion was prepared according to the following formulation and manufacturing method.
(Prescription)
Ingredient Mass%
Kanka extract 0.05
Aspartic acid 1.0
Tocopherol acetate 0.01
Glycerin 4.0
1,3-butylene glycol 4.0
Ethanol 8.0
Polyoxyethylene (60) hydrogenated castor oil 0.5
Methylparaben 0.2
Citric acid 0.05
Sodium citrate 0.1
Fragrance 0.05
Purified water Amount that makes 100 in total (production method)
Aspartic acid, glycerin, 1,3-butylene glycol, citric acid and sodium citrate were dissolved in purified water to obtain a purified aqueous solution. Separately, citrus extract, tocopherol acetate, polyoxyethylene (60) hydrogenated castor oil, methylparaben and fragrance are dissolved in ethanol, solubilized by adding to the purified aqueous solution, filtered, and lotion Prepared.

Example 16
A lotion was prepared according to the following formulation and manufacturing method.
(Prescription)
Ingredient Mass%
[A: alcohol phase]
Ethanol 5.0
Polyoxyethylene (50) oleyl ether 2.0
2-Ethylhexyl-p-dimethylaminobenzoate 0.18
Kanka extract 0.1
Fragrance 0.05
[B: aqueous phase]
1,3 butylene glycol 9.5
Sodium pyrrolidonecarboxylate 0.5
Whey extract 5.0
Nicotinamide 0.3
Glycerin 5.0
Hydroxypropyl-β-cyclodextrin 1.0
Ethylenediamine hydroxyethyl triacetate trisodium 1.0
Lysine 0.05
Tranexamic acid 1.0
Purified water Amount that makes 100 in total (production method)
The alcohol phase of A was added to the aqueous phase of B and solubilized to prepare a lotion.

Claims (3)

  A skin external preparation or skin cosmetic comprising, as an active ingredient, an extract or an extract obtained by extracting water or a lower aliphatic alcohol or a hydrate thereof from a fleshy stalk of kankanikuyuyo.   Ethinacoside, acteoside, cancanoside F, cancanoside G, cancanoside H, cancanoside A, contained in an extract or extract obtained by extracting from the fleshy stalk of kankanikusuyo with water or a lower aliphatic alcohol or its hydrate. The skin external preparation or skin cosmetic according to claim 1, comprising at least one selected from the group consisting of: Cancanoside B, Cancanoside C, Cancanoside D, Cancanol, and Cancanoside E.   The content of the extract or extract from the fleshy stalk of Kankanikuyo in the external preparation for skin or skin cosmetic is 0.001% by mass to 20% by mass as a solid content. Topical skin preparations or skin cosmetics.