JP3431383B2 - Melanin inhibitor - Google Patents

Melanin inhibitor

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Publication number
JP3431383B2
JP3431383B2 JP02670996A JP2670996A JP3431383B2 JP 3431383 B2 JP3431383 B2 JP 3431383B2 JP 02670996 A JP02670996 A JP 02670996A JP 2670996 A JP2670996 A JP 2670996A JP 3431383 B2 JP3431383 B2 JP 3431383B2
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JP
Japan
Prior art keywords
extract
melanin
duck
water
extraction
Prior art date
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JP02670996A
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Japanese (ja)
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JPH09221429A (en
Inventor
利彦 大多和
保徳 藤原
誠 細川
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T Hasegawa Co Ltd
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T Hasegawa Co Ltd
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Priority to JP02670996A priority Critical patent/JP3431383B2/en
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  • Medicines Containing Plant Substances (AREA)

Description

【発明の詳細な説明】 【0001】 【発明の属する技術分野】本発明は、しみ、そばかすな
どの色素沈着症の原因となるメラニン抑制剤に関し、さ
らに詳しくはカモカモを水、親水性有機溶媒またはこれ
らの混合物で抽出処理することにより得られるカモカモ
の抽出物を有効成分として含有するメラニン抑制剤に係
るものである。 【0002】 【従来の技術】しみ、そばかす、肝斑および日焼け後の
色素沈着は、加齢に伴い発生、増加あるいは消失しにく
くなり、中高年齢層の肌の悩みのひとつとなっている。
これらの色素沈着症の発症メカニズムは、いまだ明確に
はされていないが、紫外線、メラノサイト刺激ホルモン
(MSH)などの作用によりメラノサイトのメラニン合
成機能が亢進したためと考えられている。また、表皮角
化細胞(ケラチノサイト)の加齢に伴う角化遅延傾向
も、メラニンの表皮外への排泄速度を遅延させ、メラニ
ン合成機能の亢進と合わせて、表皮内のメラニン顆粒密
度の増加によるものと考えられている。さらにそれらの
色素沈着部は局部的に存在し、周囲の正常皮膚色と明ら
かな差異が生じることから、局部的なメラニン合成機能
の亢進、あるいはメラニン合成をコントロ−ルする機構
の変調の結果とも考えられている。従来から、これらの
後天的な色素、すなわちメラニンの沈着部を正常な皮膚
色にまで回復させる研究が行われ、これまでに数多くの
提案が報告されている。 【0003】例えば、アシタバの茎、葉または根の熱水
またはエタノール抽出物を化粧料基剤に配合してなる、
メラニン抑制作用を有する色白化粧料(特開昭60−2
09509号公報参照)、オグルマ及び/又はその同属
植物の抽出物を含有してなる、皮膚の炎症を防止、緩和
すると共に、日焼けによる皮膚の黒色化、シミ、ソバカ
スの防止及び改善効果をも有する皮膚外用剤(特開昭6
3−303919号公報参照)、また霊芝の子実体から
の抽出成分を配合した化粧料であって、メラニン生成抑
制作用、メラニン淡色化作用を有する製剤(特開平2−
124809号公報参照)等が提案されている。 【0004】 【発明が解決しようとする課題】しかしながら、これら
のメラニン抑制剤の抑制効果は、必ずしも満足できるも
のではなく、更に優れた効果を有するメラニン抑制剤の
開発が強く求められている。 【0005】 【課題を解決するための手段】そこで本発明者らは上記
課題を解決するため、安全性、安定性が高く、メラニン
抑制効果に優れた機能を有するメラニン抑制物質、
該機能を有する種々の植物の抽出物について鋭意研究の
結果、カモカモ(MYRCIARIA DUBIA)を
水、親水性有機溶媒またはこれらの混合物で抽出処理す
ることにより得られるカモカモの抽出物が、メラニンの
生成を抑制する機能を保有すること、美白作用、さらに
は保湿作用、皮膚の老化防止にも有効であることを見い
だし、本発明に至った。 【0006】カモカモはフトモモ科に属する食用植物で
あって、ペルー東部からブラジル西北部のアマゾン盆地
の河岸又は流水のある湖沼の岸に生える潅木で、その果
実のビタミンC含有量はレモンの約100倍に達すると
言われ、又、ミネラルの含有量も高く、その他有効成分
が多く含まれていることが知られている。現地ではその
果実をそのまま食用に、または果汁を絞ってジュースな
どとして飲用に供されている。カモカモは、上述のよう
にフトモモ科の果実であり、本発明においては、生、半
乾燥、乾燥品のいずれでも使用でき、これらの原料は粉
砕して用いるのが好適である。 【0007】すなわち、本発明は、カモカモを水、親水
性有機溶媒またはこれらの混合物で抽出処理することに
より得られるカモカモの抽出物を有効成分として含有す
るメラニン抑制剤を要旨とするものである。 【0008】 【発明の実施の形態】本発明のカモカモエキスは、水、
親水性有機溶媒またはこれらの混合物で抽出処理するこ
とにより容易に得ることができる。かかる抽出処理に用
いる親水性有機溶媒としては、例えば、メタノール、エ
タノール、n−プロピルアルコール、アセトン、プロピ
レングリコール、グリセリンなどの溶媒を挙げることが
できる。これらの溶媒は、例えば水と任意の割合で混合
した含水溶剤の形で用いることもできるが、約10〜約
99.9%の含水溶媒で抽出を行うのが好ましい。 【0009】抽出操作および抽出条件は用いる抽出溶剤
の種類に応じて、種々変更することができるが、抽出溶
剤として前記の如き親水性有機溶媒またはそれと水との
混合液を用いて抽出を行う場合には、例えば、カモカモ
に約1.5倍〜約50倍量の溶媒を加え、室温ないし使
用溶媒の沸点温度で、約5分ないし約24時間、静置も
しくは撹拌して行うことができる。このようにして抽出
操作を行った後、例えば遠心分離、ろ過、圧搾その他の
固液分離手段によって不溶性残渣を除去することによ
り、カモカモエキスを得ることができる。さらに必要な
らば、不溶性抽出残渣に抽出溶剤を加えて、同様の操作
をくり返し、抽出することもできる。また更に、該抽出
操作の際に、ペクチナーゼなどの酵素を添加して、酵素
処理を行うことにより、抽出効率を上げることができ
る。 【0010】本発明においては、カモカモから上記のご
とき方法で得られる抽出液をそのまま本発明の抽出物と
して使用してもよく、或いは該抽出液から溶剤を除去す
ることにより得られる濃縮物、さらには、それを減圧乾
燥または凍結乾燥して得られる乾燥物を本発明の抽出物
として利用することもできる。 【0011】さらに、上記のような方法によって得られ
る抽出物を、シリカゲル、活性炭、活性アルミナ、ベン
トナイト、酸性白土、珪藻土などの吸着剤により、脱
色、脱臭を行ってもよい。吸着操作は、抽出物をそのま
まあるいは濃縮した濃縮物を水など適当な溶媒に溶解し
た溶液に、適当量の吸着剤を添加することにより行うこ
とができる。吸着処理液は、そのまま或いは減圧下に濃
縮して得られる濃縮液、さらにそれを、例えば減圧乾燥
または凍結乾燥して得られる乾燥物を抽出物として利用
することもできる。 【0012】さらにまた、上記の脱色、脱臭処理前また
は処理後の抽出物を、例えば適当量の水に溶解して、ス
チレンビニルベンゼン、メタクリル酸エステルなどの樹
脂よりなる合成吸着剤を用いて有効成分を吸着処理し、
次いで例えば、エタノール、メタノール、アセトン、酢
酸エチルのごとき親水性有機溶媒あるいはこれらと水と
の混合溶媒で脱着操作を行って精製し、カモカモの抽出
物中の有効成分を濃縮して、本発明の抽出物として利用
することもできる。吸着、脱着操作は、バッチ方式また
はカラムによる連続方式で行うことができ、好ましくは
カラムによる連続方式が採用される。この操作後の脱着
液は、そのまま本発明の抽出物として利用することがで
きるが、また減圧下に濃縮して得られる濃縮液、更にこ
れを、例えば減圧乾燥または凍結乾燥して得られる乾燥
物を本発明の抽出物として利用することもできる。 【0013】以上述べたようにして調製されるカモカモ
の抽出物は、優れたメラニン抑制能を有する。本発明の
メラニン抑制剤は、その他の任意成分を加えて種々の形
態にすることができるが、一般的には例えば、ロ−ショ
ン、乳液状、クリ−ム状、軟膏状、スティック状、有機
溶剤による溶液状、パック状、ゲル状等とすることが好
ましい。また、該カモカモ抽出物以外に配合することの
できるその他の任意成分としては、化粧品に通常配合し
て使用されている成分、例えば、精製水、エタノール、
油性物質、保湿剤、美白剤、増粘剤、防腐剤、乳化剤、
薬効成分、粉体、紫外線吸収剤、色素、香料、乳化安定
剤、pH調整剤等を挙げることができる。上記の保湿剤
としては、例えば、γ−リノレイン酸、スフィンゴ脂
質、キサンタンガム、ヒアルロン酸、牛骨髄抽出物、水
溶性コラーゲン、キチン、キトサン、ムチン、乳酸菌培
養液、ビフィズス菌代謝物、酵母発酵代謝物、酵母抽出
物、ピロリドンカルボン酸、プルラン、マルトース、ヘ
チマエキス;美白剤としては、例えば、プラセンターエ
キス、コウジ酸、桑白皮、当帰抽出物、ワレモコウ、イ
ブキトラノオ、ビタミンCおよびその誘導体などと本発
明のカモカモ抽出物を併用すれば、美白作用、保湿作
用、皮膚の老化防止の効果を増強することができる。こ
のようにして得られる本発明のメラニン抑制剤は、皮膚
のしみ、そばかす、日焼け後の色素沈着部などの患部に
局所的に適用される。また一般にその使用量は、特に限
定されないが、通常クリーム状、軟膏状製剤の場合は皮
膚面1cm2当たり約0. 5〜10mg、液状製剤の場
合は約1〜15mg程度の範囲で使用される。 【0014】 【実施例】以下、実施例により、本発明の実施の態様に
ついて更に詳細に説明する。 実施例 (カモカモ抽出物試料の調製)カモカモ粉砕物50g
に、50%エタノール水溶液400gを添加し、時々撹
拌しながら3日間抽出処理を行い、遠心分離、濾過処理
して濾液350gを得た。この濾液を減圧下に濃縮乾固
して乾燥抽出物45gを得た。この抽出物について、メ
ラニン生成を抑制する能力即ち、チロシナーゼ活性阻害
能を測定する。 (チロシナーゼ活性阻害能の測定)上記の抽出物試料
0. 4mgに酵素溶液(和光純薬工業社製 25,000unit
のチロシナーゼを 0.1Mリン酸緩衝液(pH7.0)20 ミリリ
ットルに溶解したもの)0.1ミリリットルを加え、さ
らに 0.1Mリン酸緩衝液(pH7.0)を加え4ミリリットル
とし、これを25℃で10分間インキュベートした。こ
れに予め25℃に保っておいた基質溶液[L-DOPA( 東京
化成社製)198.0mgを 0.1Mリン酸緩衝液(pH7.0)100ミ
リリットルに溶解したもの]1. 0ミリリットルを加え
10分間反応せしめた。次いで、475 nmにおける吸光
度を測定しこれをODC 、さらに加熱失活させた前記酵
素を用いて同様に反応させたものの吸光度ODD 、試料
の代わりに水を加えた時の吸光度をODA 、試料の代わ
りに水を加え、かつ失活酵素を用いた時の吸光度をOD
B として、これらを測定し、次の数式によるチロシナー
ゼ活性阻害率を求めた。 チロシナーゼ活性阻害率(%)=[{ODA-ODB-(ODC-O
DD) }/(ODA-ODB )]× 100=82. 6(%) ただし、ODA :(水+酵素+基質)の吸光度 ODB :(水+失活酵素+基質)の吸光度 ODC :(試料+酵素+基質)の吸光度 ODD :(試料+失活酵素+基質)の吸光度 この結果からカモカモの抽出物は、優れたメラニン生成
抑制機能を有することがわかる。 【0015】 【発明の効果】本発明によれば、メラニン生成を効果的
に抑制する優れたチロシナーゼ活性阻害能を有するメラ
ニン抑制剤を提供することができる。
Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a melanin inhibitor which causes pigmentation such as spots and freckles, and more particularly to a duck with water, a hydrophilic organic solvent or this
The present invention relates to a melanin inhibitor containing, as an active ingredient, an extract of ducks obtained by performing an extraction treatment with these mixtures . [0002] Blots, freckles, liver spots and pigmentation after sunburn are less likely to occur, increase or disappear with age, and are one of the concerns for middle-aged and older people.
Although the mechanism of the onset of these pigmentation diseases has not been elucidated yet, it is considered that the melanocyte synthesizing function of melanocytes was enhanced by the action of ultraviolet rays, melanocyte stimulating hormone (MSH) and the like. In addition, the tendency of keratinocytes (keratinocytes) to delay keratinization with aging also delays the rate of excretion of melanin to the outside of the epidermis and increases the density of melanin granules in the epidermis together with the enhancement of melanin synthesis. Is believed to be something. Furthermore, since these pigmented areas are present locally and have a clear difference from the surrounding normal skin color, the pigmentation site is locally enhanced in melanin synthesizing function or the result of modulation of the mechanism controlling melanin synthesis. It is considered. Conventionally, studies have been conducted to restore these acquired pigments, that is, melanin deposits, to a normal skin color, and many proposals have been reported so far. [0003] For example, a hot water or ethanol extract of the stem, leaf or root of Ashitaba is mixed with a cosmetic base,
Fair-skin cosmetics having a melanin-suppressing action (JP-A-60-2
No. 09509), which contains an extract of oguruma and / or its congener, prevents and alleviates skin inflammation, and also has an effect of preventing and improving skin blackening due to sunburn, spots and freckles. External preparation for skin
JP-A-3-303919), and a cosmetic composition containing an extractive component from the fruiting body of Reishi, which has a melanin production inhibitory action and a melanin lightening action (Japanese Unexamined Patent Publication No.
124809) and the like have been proposed. [0004] However, the inhibitory effects of these melanin inhibitors are not always satisfactory, and there is a strong demand for the development of melanin inhibitors having even better effects. [0005] For Therefore SUMMARY OF THE INVENTION The present inventors to solve the above problems, safety, high stability, melanin inhibitors having a function having excellent melanin suppression effect, the function especially As a result of diligent research on extracts of various plants having sorghum , duck (MYRCIARIA DUBIA)
Extract with water, a hydrophilic organic solvent or a mixture of these.
Extract of Kamoka model obtained by Rukoto is to possess a function of inhibiting the formation of melanin, whitening effect, and more moisturizing effect, it found that it is also effective in anti-aging skin, leading to the present invention . Camo duck is an edible plant belonging to the family Myrtaceae and is a shrub that grows on the banks of the Amazon basin or the shores of flowing lakes in the eastern part of Peru to the northwestern part of Brazil. It is known that the amount of the active ingredient is increased, and that the mineral content is high and other active ingredients are included in a large amount. In the field, the fruit is used as it is for eating, or the fruit juice is squeezed for drinking as juice. The ducks are fruits of the family Myrtaceae as described above, and in the present invention, any of raw, semi-dried, and dried products can be used, and these raw materials are preferably ground and used. [0007] That is, the present invention provides a duck with water and hydrophilic water.
Extraction with a neutral organic solvent or a mixture of these
The gist of the present invention is a melanin inhibitor containing as an active ingredient the resulting extract of ducks. BEST MODE FOR CARRYING OUT THE INVENTION
It can be easily obtained by performing an extraction treatment with a hydrophilic organic solvent or a mixture thereof. Examples of the hydrophilic organic solvent used in the extraction treatment include solvents such as methanol, ethanol, n-propyl alcohol, acetone, propylene glycol, and glycerin. These solvents can be used, for example, in the form of a hydrated solvent mixed with water in an arbitrary ratio, but it is preferable to perform extraction with about 10 to about 99.9% of a hydrated solvent. The extraction operation and the extraction conditions can be variously changed according to the type of extraction solvent used. However, when the extraction is carried out using a hydrophilic organic solvent as described above or a mixed solution thereof with water as described above. For example, about 1.5 times to about 50 times the amount of a solvent is added to duck and the mixture is allowed to stand or stirred at room temperature to the boiling point of the solvent used for about 5 minutes to about 24 hours. After performing the extraction operation in this manner, a duck extract can be obtained by removing insoluble residues by, for example, centrifugation, filtration, squeezing, or other solid-liquid separation means. If necessary, an extraction solvent can be added to the insoluble extraction residue, and the same operation can be repeated to perform the extraction. Furthermore, at the time of the extraction operation, by adding an enzyme such as pectinase and performing the enzyme treatment, the extraction efficiency can be increased. In the present invention, the extract obtained from the duck by the above method may be used as it is as the extract of the present invention, or a concentrate obtained by removing the solvent from the extract, The dried product obtained by drying it under reduced pressure or freeze-drying can also be used as the extract of the present invention. Further, the extract obtained by the above method may be decolorized and deodorized with an adsorbent such as silica gel, activated carbon, activated alumina, bentonite, acid clay, and diatomaceous earth. The adsorption operation can be performed by adding an appropriate amount of an adsorbent to a solution of the extract as it is or a concentrate obtained by dissolving the extract in a suitable solvent such as water. As the adsorption treatment liquid, a concentrated liquid obtained as it is or by concentrating it under reduced pressure, and a dried product obtained by drying it under reduced pressure or freeze-drying can be used as an extract. Further, the extract before or after the above decolorization and deodorization treatment is dissolved in, for example, an appropriate amount of water, and is effectively treated with a synthetic adsorbent made of a resin such as styrene vinyl benzene or methacrylic acid ester. Absorb components
Then, for example, ethanol, methanol, acetone, purified by performing a desorption operation with a hydrophilic organic solvent such as ethyl acetate or a mixed solvent of these and water, and concentrating the active ingredient in the extract of duck, the present invention It can also be used as an extract. The adsorption and desorption operations can be performed in a batch mode or a continuous mode using a column, and a continuous mode using a column is preferably employed. The desorbed solution after this operation can be used as it is as the extract of the present invention, and it is also a concentrated solution obtained by concentrating under reduced pressure, and further, for example, a dried product obtained by drying under reduced pressure or freeze-drying Can also be used as the extract of the present invention. [0013] The extract of ducks prepared as described above has excellent melanin-suppressing ability. The melanin inhibitor of the present invention can be made into various forms by adding other optional components. In general, for example, lotion, emulsion, cream, ointment, stick, organic It is preferable to use a solvent-based solution, pack, gel, or the like. In addition, as other optional components that can be blended in addition to the duck extract, components commonly blended and used in cosmetics, for example, purified water, ethanol,
Oily substances, humectants, whitening agents, thickeners, preservatives, emulsifiers,
Examples include medicinal ingredients, powders, ultraviolet absorbers, dyes, fragrances, emulsion stabilizers, pH adjusters, and the like. Examples of the humectant include, for example, γ-linoleic acid, sphingolipid, xanthan gum, hyaluronic acid, bovine bone marrow extract, water-soluble collagen, chitin, chitosan, mucin, lactic acid bacteria culture solution, bifidobacterium metabolite, yeast fermentation metabolite , Yeast extract, pyrrolidonecarboxylic acid, pullulan, maltose, loofah extract; Examples of whitening agents include placenta extract, kojic acid, mulberry bark, toki extract, waremokou, ivukitorano, vitamin C and derivatives thereof. When the extract of the duck of the present invention is used in combination, the whitening effect, the moisturizing effect, and the effect of preventing skin aging can be enhanced. The melanin inhibitor of the present invention thus obtained is applied topically to affected areas such as skin spots, freckles, and pigmented areas after sunburn. In general, the amount of use is not particularly limited, but it is usually used in the range of about 0.5 to 10 mg per 1 cm 2 of the skin surface in the case of a cream or ointment, and about 1 to 15 mg in the case of a liquid preparation. . The embodiments of the present invention will be described in more detail with reference to the following examples. Example (Preparation of duck duck extract sample) 50 g of duck duck
Then, 400 g of a 50% aqueous ethanol solution was added thereto, and an extraction treatment was carried out for 3 days with occasional stirring, followed by centrifugation and filtration to obtain 350 g of a filtrate. The filtrate was concentrated to dryness under reduced pressure to obtain 45 g of a dry extract. This extract is measured for its ability to suppress melanin production, that is, its ability to inhibit tyrosinase activity. (Measurement of tyrosinase activity inhibiting ability) An enzyme solution (25,000 units manufactured by Wako Pure Chemical Industries, Ltd.) was added to 0.4 mg of the above extract sample.
Tyrosinase was dissolved in 20 ml of 0.1 M phosphate buffer (pH 7.0)), 0.1 ml was added, and 0.1 M phosphate buffer (pH 7.0) was further added to make 4 ml. For 10 minutes. To this was added 1.0 ml of a substrate solution (198.0 mg of L-DOPA (manufactured by Tokyo Chemical Industry Co., Ltd.) dissolved in 100 ml of 0.1 M phosphate buffer (pH 7.0)) previously kept at 25 ° C. Allowed to react for minutes. Then, the absorbance at 475 nm was measured, and this was measured as OD C , and further, the absorbance OD D of the same reaction using the enzyme inactivated by heating, the absorbance when water was added instead of the sample as OD A , Add the water in place of the sample and use the inactivated enzyme to measure the absorbance at OD.
These were measured as B , and the tyrosinase activity inhibition rate was determined by the following equation. Tyrosinase activity inhibition rate (%) = [{OD A -OD B- (OD C -O
D D)} / (OD A -OD B)] × 100 = 82. 6 (%) However, OD A :( water + enzyme + substrate) absorbance OD B :( water + inactivation enzyme + substrate) absorbance of the OD C : absorbance of (sample + enzyme + substrate) OD D : absorbance of (sample + inactivated enzyme + substrate) From this result, it can be seen that the extract of duckling has an excellent melanin production inhibitory function. According to the present invention, a melanin inhibitor having an excellent ability to inhibit tyrosinase activity, which effectively suppresses melanin production, can be provided.

───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 FI A61P 43/00 101 A61P 43/00 101 (56)参考文献 特開 平2−200610(JP,A) 特開 昭51−142514(JP,A) J.Sci,FOOd,Agric, 1993,Vol.61,pp.349−351 (58)調査した分野(Int.Cl.7,DB名) A61K 35/78 A61K 7/00 BIOSIS(DIALOG) CA(STN) MEDLINE(STN)──────────────────────────────────────────────────続 き Continuation of the front page (51) Int.Cl. 7 Identification symbol FI A61P 43/00 101 A61P 43/00 101 (56) References JP-A-2-200610 (JP, A) JP-A-51-142514 (JP, A) Sci, FOOd, Agric, 1993, Vol. 61, pp. 349-351 (58) Fields investigated (Int. Cl. 7 , DB name) A61K 35/78 A61K 7/00 BIOSIS (DIALOG) CA (STN) MEDLINE (STN)

Claims (1)

(57)【特許請求の範囲】 【請求項1】 カモカモを水、親水性有機溶媒またはこ
れらの混合物で抽出処理することにより得られるカモカ
モの抽出物を有効成分として含有するメラニン抑制剤。
(57) [Claims] [Claim 1] A duck is added to water, a hydrophilic organic solvent or
A melanin inhibitor comprising, as an active ingredient, an extract of ducks obtained by performing an extraction treatment with these mixtures .
JP02670996A 1996-02-14 1996-02-14 Melanin inhibitor Expired - Lifetime JP3431383B2 (en)

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US8048456B2 (en) 2009-08-28 2011-11-01 Mary Kay Inc. Skin care formulations
US10130673B2 (en) 2006-01-19 2018-11-20 Mary Kay Inc. Compositions comprising kakadu plum extract or acai berry extract
WO2019149864A1 (en) 2018-01-30 2019-08-08 Isp Investments Llc Myrciaria dubia fruit extracts rich in organic acids, cosmetic compositions comprising same and cosmetic uses thereof

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JPH11246336A (en) * 1998-02-27 1999-09-14 Ichimaru Pharcos Co Ltd Activated oxygen scavenger and skin beautifying cosmetic composition
JP2001031558A (en) * 1999-05-19 2001-02-06 Kose Corp Skin lotion
JP2001031580A (en) * 1999-05-19 2001-02-06 Kose Corp Preparation for external use for skin
JP3635081B2 (en) * 2002-12-13 2005-03-30 株式会社ニチレイ Whitening agents, antioxidants, collagenase activity inhibitors, hyaluronidase activity inhibitors, anti-aging agents, external preparations for skin, cosmetics and foods
ECSP11011303A (en) 2011-09-03 2013-03-28 Pharmabrand S A ANTIOXIDANT, NATURAL ANTIGRIPAL COMPOSITION
JP6378969B2 (en) * 2014-08-19 2018-08-22 株式会社山田養蜂場本社 Whitening composition

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J.Sci,FOOd,Agric,1993,Vol.61,pp.349−351

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US10918591B2 (en) 2006-01-19 2021-02-16 Mary Kay Inc. Compositions comprising kakadu plum extract or acai berry extract
US10675323B2 (en) 2006-01-19 2020-06-09 Mary Kay Inc. Topical compositions comprising acai berry extract
US10668124B2 (en) 2006-01-19 2020-06-02 Mary Kay Inc. Compositions comprising kakadu plum extract or acai berry extract
US9833642B2 (en) 2009-08-28 2017-12-05 Mary Kay Inc. Skin care formulations
US10434340B2 (en) 2009-08-28 2019-10-08 Mary Kay Inc. Skin care formulations
US8048456B2 (en) 2009-08-28 2011-11-01 Mary Kay Inc. Skin care formulations
US8895082B2 (en) 2009-08-28 2014-11-25 Mary Kay Inc. Skin care formulations
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