JPH1129489A - Anti scurf agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject agent having anti-Pityrosprum ovale properties, high safety and slight adverse effects, by including a pressed substance and/or an extract derived from a plant belonging to the genus Calanthe. SOLUTION: This agent contains about 0.005-0.5 of a pressed substance (squeezed juice) and/or an extract (alcohol extract) containing at least one of especially Tryptanthrin, Calanthoside A and Calanthoside B derived from a plant selected from preferably Calanthe discolor Lindl, Calanthe aristurifera and Calanthe reflexa and has antimcrobial actions especially on Malasezia furfur as a scurf causative.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an anti-dandruff agent, particularly to an anti-dandruff agent containing a pressed product and / or an extract from a natural plant as an active ingredient.

[0002]

2. Description of the Related Art In general, dandruff is caused by secretions from sebaceous glands, sweat glands, and the like, and exfoliation of the stratum corneum based on skin metabolism. Plant-derived antidandruff agents, hair restorers, hair loss inhibitors, and the like that suppress these secretions and keratinization are widely known. On the other hand, it is said that secretion and keratinization are promoted by the propagation of microorganisms which are present on the scalp and cause dandruff. As an antibacterial substance against dandruff, it is common to add an antibiotic or a chemically synthesized substance, and these substances have a strong antibacterial action but also have strong side effects. For this reason, in recent years, a highly safe plant-derived anti-dandruff agent has been demanded and proposed. For example, cypress plants (Japanese Unexamined Patent Publication No.
-150208), Rutaceae plants (JP-A-7-178)
No. 35) and other Japanese and Chinese herbal medicines (JP-A-3-176413).
And Japanese Patent Application Laid-Open No. 3-176414) are known.

[0003]

The above-mentioned anti-dandruff agents are mainly used for the dandruff fungus Pytyrosprum ov.
Although the antimicrobial activity against ole has been studied, the present inventors have conducted intensive studies on the pressed product and / or extract of the genus Ebine and found that Malas, one of the dandruff-causing bacteria, was
The present inventors have found that they have an antibacterial effect on ezia furfur, and have reached the present invention. The present invention is based on the above findings and has an object to provide a plant-derived anti-dandruff fungicide which has anti-dandruff properties and is safe.

[0004]

The anti-dandruff agent of the present invention, which has achieved the above object, is characterized in that it contains a pressed product and / or an extract from a plant belonging to the genus Ebine.

In the following, in the present invention, the terms "pressed material" and "extract" refer not only to pressed juice (liquid) and extract, but also to
It also includes their concentrated solutions and purified products.

[0006] Compresses and / or extracts from plants of the genus Ebine include indole compounds such as indirubin, trybutantrine, currantside A and currantside B,
Alternatively, it includes a dihydrophenanthrene compound such as carantenol. In addition, vomiforol and adenosine are also included. The chemical structure of the indole compound is as follows: indirubin is represented by chemical formula 1, and tributanthrin is represented by chemical formula 2.
The calantaside A is shown in Chemical formula 3, and the currant side B is shown in Chemical formula 4. Caranthenol, one of the dihydrophenanthrene compounds, is shown in Chemical formula 5. Adenosine is shown in Chemical formula 6, and vomiforiol is shown in Chemical formula 7. Among them, tryptantrine, currant side A, currant side B, and carantenol clearly show anti-dandruff properties.

[0007]

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[0008]

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[0009]

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[0010]

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[0011]

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[0012]

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[0013]

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[0014]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, the following orchids of the family Orbaceae can be exemplified. (1) diebine type: diebine (C. discocol)
r), C. sieboldii, C. aristulifera, C. amamiana, C. ezine (C. iz)
u-insularis), Salmen ebine (C. tr
icarinata), Skull shrimp (C. tok)
unoshimaensis), Alisan Ebine (C.
arisanensis), Hosobanana ebine (C. gr)
aciliflora), Himalayan house lobster (C.
plantginea), chlororeuka (C. chl)
oroleuca), Togaribaebin (C. cauda)
tilabella). (2) Tin free vine type: tin free vine (C. liu
kiensis), C. densi (C. densi)
flora), C. pulchra. (3) Taganelan type: Taganelan (C. bungoan)
a), Whiteana (C. whiteana). (4) Sakurajima shrimp type: Sakurajima shrimp (Co.
blanceolata), Manny (C. mann)
i). (5) Kiso ebine type: xo ebine (C. schlech
teri), C. alpina. (6) Kinseiran type: Kinseiran (C.nippo)
nica). (7) Hirohanokaran type: Hirohanokaran (C. jap
onica). (8) Asahyebine type: Asahyebine (C. hatto
ri). (9) Natsuebine type: Natsuebine (C. reflex
a). (10) Turran type: Turran (C. furcat
a), Long-tailed shrimp (C. longicarat)
a), Okinawa shrimp (C. okinawaensi)
s), masker (C. mascara), Madagascariensis, C. natalensis. (11) Toxaran type: Toxaran (C. venust
a), C. longipes, C. biloba. (12) Pastetor type: Vestita (C. vestit
a), Rosea (C. rosea), Hermerie (C. el)
meri), C. rubens, C. cardilossa. Particularly, plants belonging to the genus Ebine include diebine (Cal
anthe color Lindl. ), And extracts from C. reflexa and / or the like have strong anti-dandruff properties.

The pressed product and / or extract of Ebine, which is an active ingredient of the present invention, is obtained by pressing or extracting at least one kind of Ebine belonging to the genus Ebine from whole plants such as flowers, roots, stems and leaves. . As an extraction solvent at the time of extraction, lower alkyl alcohol, particularly ethyl alcohol is desirable from the viewpoint of safety. Ethyl alcohol may be pure ethyl alcohol, but industrially, hydrated ethyl alcohol is preferred. Spirits such as distilled spirits can also be used. The alcohol concentration in the hydrous ethyl alcohol is not particularly limited, but is practically about 30 to 90%, preferably about 40 to 70%.

In order to produce a pressed product from shrimp, mud adhering to the rhizome of shrimp and the like is dropped, and the whole plant is washed with water.
Next, it is roughly cut with a cutter, a mixer or the like, and squeezed by a press. The compression pressure of the press is 500-500.
It may be set to about 0 kg / cm ² . The squeezed juice can be used as it is or purified or diluted.

In order to produce an extract from shrimp, a conventional solvent extraction method may be used. For example, after washing the shrimp with water, it is directly immersed in the alcohol solvent without pressing. For about 10,000 g of ebine, for example, 20
Approximately 20,000 ml of 90% ethyl alcohol is mixed and stored tightly in a cool and dark place for 7 to 10 days, followed by solid-liquid separation and filtration. Desirably, the mixture is squeezed once as described above, and then mixed and extracted from the squeezed juice with 20-90% aqueous ethyl alcohol at a ratio of about 1: 1 (volume). The obtained extract is more effective when sealed and aged in a cool and dark place for about 1 to 2 weeks.

The squeezed product and / or extract from the plant of the genus Ebine can be used as an anti-dandruff agent by adding it to cosmetics, shampoos, hair restorers, soaps and the like. The concentration of the squeezed product and / or the extract is 0.005 to 0.5.
It is effective if about 5% is contained.

Mixing of the shrimp and / or extract with other compositions can be selected at any time according to the type of the composition. For example, some vitamins are water-soluble and some are oil-soluble, and include water, warm water, ethyl alcohol or a mixture thereof, including the relationship with the used animal and vegetable oils, and will be described later in general. It is desirable to follow the embodiment.
As an operation, heating is performed in principle with hot water and stirring if necessary.

[0020]

Example 1 As a raw material, diebine native to Yamano, Miyazaki Prefecture was used. First, mud attached to the rhizomes of shrimp was dropped, and the whole plant was washed with water. Then, it was roughly cut and squeezed using a compressor (model KS-1 manufactured by Komagata Kikai Seisakusho). The yield of the squeezed juice was 15-20% by weight based on the whole plant.

[0021]

Example 2 1 kg of the roughly cut shrimp of Example 1 was placed in 3 liters of 95% ethanol and allowed to stand at room temperature for 3 weeks to obtain a shrimp alcohol extract.

[0022]

Example 3 1 kg of the roughly cut shrimp of Example 1 was placed in 3 liters of 35% ethyl alcohol and allowed to stand at room temperature for 3 weeks to obtain a 35% alcohol extract of shrimp.

[0023]

[Analysis Example] (Component analysis) The specific components in the squeezed juice and the extract of Examples 1, 2 and 3 were confirmed by thin-layer chromatography. Confirmation method is to take 20 μl of each extract and use a glass plate for thin layer chromatography (Merck, silica gel 60F).
254), and chloroform: methanol = 10: 1 was used as a developing solvent. Detection was by UV lamp irradiation. FIG. 1 shows thin-layer chromatographic patterns of typical component compounds.

[0024]

[Test Example 1] (Antibacterial activity of extract) Using the juices and extracts of Examples 1, 2 and 3 as stock solutions, the solvent was completely distilled off at 45 ° C under reduced pressure, and each of the pressed shrimp of Ebine and A dried product of the extract was obtained. Next, it was dissolved in purified water and sterilized by filtration using a sterilized membrane filter. Each sample of the filtered shrimp was diluted with sterile purified water. 7.5 μl of a sample was added to 1 ml of a liquid medium to obtain a test medium. At this time, the dilution concentration of the sample was the following two-fold dilution. The liquid medium used was
Peptone 0.5%, yeast extract 0.3%, glucose 1.
0%, olive oil 1.0%, pH was 6.0.
For inoculation and culture of the bacteria, the cryopreserved bacterial solution was thawed, 10 μl of the solution was inoculated into 10 ml of a liquid medium, and cultured with shaking overnight. Depending on the number of bacteria after overnight shaking culture measured in advance,
The number of bacteria was 106 / ml, which was used as an inoculum. 10 μl of the inoculated bacterial solution (Malaseziafurfur) was inoculated into the test medium, and the final bacterial solution volume was set at 104 cells / ml to 25 μl.
The culture was performed with shaking at 18 ° C. for 18 hours. Judgment was made invalid when the growing bacteria were turbid or sedimented visually, and the concentration when it was transparent was regarded as the effective concentration. The results are shown in Table 1 at the minimum inhibitory concentration.

[0025]

[Table 1]

From these results, it can be seen that both the squeezed juice and the extract of shrimp are Malasezi, the causative bacteria of dandruff.
It showed strong antibacterial activity against a furfur.

[0027]

[Test Example 2] (Antibacterial activity of squeezed squeezed juice and extract-containing components) It was determined after infiltration culture at 25 ° C overnight using a liquid dilution method. The judgment was based on the following criteria. Table 2 shows the results. . Criteria for positive growth: When turbidity or sediment is visually observed. . Criteria for growth inhibition: When turbidity or sediment is not visually observed.

[0028]

[Table 2]

As can be seen from Table 2, tryptanthrin, currant side A, currant side B and carantenol all have anti-dandruff properties. In particular, tryptanthrin is the same as tetracyclic acid addition salt as a control. Shows strong anti-dandruff properties.

[0030]

[Test Example 3] (Primary skin irritation test) Examples 1, 2 and 3 used for external use
Was tested for skin irritation using guinea pigs. Hartley-type guinea pigs used about 300 g females as a group of 6 animals. Before the test, anesthetize with ether, and the back is shaved widely with an electric clipper and further shaved. The depilated portion was thoroughly wiped with rubbing alcohol, and as shown in FIG. 2, one of the application sites of the sample was cut into a lattice-like wound with an injection needle. 1. Extract 10.5 ml of sample.
It was adsorbed on a 5 × 25 cm filter paper, fixed firmly with a waterproof film (manufactured by Morishita Jintansha), and then fixed with a stretchable adhesive bandage. 24 hours after application, the batch was peeled off, and the degree of erythema and edema was compared with purified water as a blank in the case of Ebine squeezed juice (Example 1). In the case of the alcohol extract (Examples 2 and 3), Only each solvent was compared as a blank.

[0031] As a result, no response to irritating the skin was observed in any of the squeezed juice and the extract of the shrimp, which was the same as that of the blank.

[0032]

[Formulation Example 1] (Shampoo) Triethanolamine lauryl sulfate 12.0 (%) Propylene glycol 4.0 Diethanolamine laurylate 2.0 Disodium ethylenediaminetetraacetate 0.1 Ethanol extract containing 50% ebine 30.0 Preservative Appropriate amount Perfume Appropriate amount Purified water balance

[0033]

EFFECTS OF THE INVENTION The anti-dandruff agent of the present invention is prepared according to the above-mentioned Formulation Example 1.
It can be expected to be used in a wide range of applications such as shampoos, cosmetics, hair tonics, soaps, etc., exhibits strong anti-dandruff action as a natural product, and has high safety and few side effects.

[Brief description of the drawings]

FIG. 1 is a simulated view showing a typical thin-layer chromatographic pattern of a component containing a pressed shrimp and / or an extract.

FIG. 2 is a mimetic diagram showing the site of application of a sample to a Hartley-type guinea pig in a skin primary irritation test.

────────────────────────────────────────────────── ─── Continued on the front page (51) Int.Cl. ⁶ Identification code FI A61K 31/71 A61K 31/71 // A61K 7/075 7/075 31/12 31/12 C07D 209/34 C07D 209/34 487 / 04 140 487/04 140 C07H 17/02 C07H 17/02

Claims (6)

[Claims] 1. An anti-dandruff agent comprising a pressed product and / or an extract from a plant belonging to the genus Ebine. 2. A compressed product from a plant belonging to the genus Ebine,
The anti-dandruff agent according to claim 1, which is a pressed juice obtained by pressing a plant belonging to the genus Ebine. 3. An extract from a plant belonging to the genus Ebine,
The anti-dandruff agent according to claim 1, which is an alcoholic extract from a plant belonging to the genus Ebine. 4. The compressed product and / or extract from the plant of the genus Ebine contains at least one indole compound selected from the group consisting of trybutanthrin, currantside A and currantside B. Item 1
The anti-dandruff agent described in the above. 5. The anti-dandruff agent according to claim 1, wherein the squeezed product and / or extract from the plant of the genus Ebine contains carantenol. 6. The plant of the genus Ebine is a plant of the genus Ebine
he discolor Lindl. ), Kirishima prawn (Calanthe aristurifer)
a), Natsuebine (Calanthe reflex)
The anti-dandruff agent according to claim 1, which is at least one selected from the group consisting of a).