JP3217741B2 - Whitening agent - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to (in aka Mehajiki) Ekihahakusa having melanin production inhibitory activity characterized by containing an extract of sunburn only after pigmentation, age spots, freckles, etc. melasma The present invention relates to a whitening agent effective for prevention and improvement.

[0002]

2. Description of the Related Art In general, skin pigmentation observed in spots, freckles, sunburn, and the like is caused by excessive production of melanin by melanin-producing cells present in the skin. To improve the pigmentation, hydroquinone and its derivatives have been used externally to decompose melanin, and arbutin, glutathione, ascorbic acid and the like have been used externally as tyrosinase activity inhibitors.

[0003]

[SUMMARY OF THE INVENTION However, by way of conventional excluding hydroquinone, for to improve this pigmentation expression of the effect is very slow, the effect is not sufficient. Although hydroquinone has been shown to be effective, its use is restricted in Japan because of allergic contact dermatitis as a side effect, and its formulation is unstable. In view of such circumstances, the present inventors have repeatedly studied to obtain a whitening agent having high safety and an excellent whitening effect. As a result, the mother plant has an excellent whitening effect, and is extremely excellent in safety. And completed the present invention based on this finding.

[0004]

That is, the present invention is a whitening agent characterized by containing an extract of a mother plant (also known as a repellent swordfish). In the present invention, the mother plant is a plant belonging to the Labiatae family widely distributed in Asia such as Japan, Taiwan, Korea, and China, and is called Leonurus sibiricus L. The whole plant decoction is taken.

[0005] The extract of beneficial mother grass used in the present invention is obtained by extracting from a part or the whole plant of leaf, stem, flower, fruit and root plants of the beneficial mother grass. Preferably, it is obtained by extracting from one or both of the leaves and stems. The preparation method is not particularly limited, and may be, for example, one extracted by heating or one extracted at ordinary temperature. Examples of the solvent to be extracted include water, lower monohydric alcohols (such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol and 2-butanol), and liquid polyhydric alcohols. (1,3-butylene glycol, propylene glycol, etc.), esters (ethyl acetate, isopropyl myristate), hydrocarbons (benzene, hexane, pentane, liquid paraffin, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (Ethyl ether, tetrahydrofuran, propyl ether, etc.), acetonitrile and the like. These solvents may be used alone or as a mixture of two or more. Preferably, one or two or more of water or a water-soluble solvent (a solvent that can be mixed with water at an arbitrary ratio; for example, ethanol, 1,3-butylene glycol, propylene glycol, and the like) may be used. .

[0006] The extract of the primrose may be used as it is, or may be used after concentration, dilution, filtration, etc., if necessary.

The whitening agent of the present invention contains oils and fats, waxes, hydrocarbons,
Fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, humectants, powders, ultraviolet absorbers, thickeners, pigments, antioxidants, beauty ingredients, chelating agents And the like.

The whitening agent of the present invention can be used in any of pharmaceuticals, quasi-drugs, and cosmetics. Examples of the dosage form include lotions, creams, emulsions, gels, aerosols, essences, packs, and the like. Those applied to the skin such as cleaning agents, bath agents, foundations, powdering, lipsticks, ointments, cataplasms, pastes, plasters and the like can be mentioned.

[0009]

[0010]

EXAMPLES Next, the present invention will be described in detail with reference to examples, but the present invention is not limited thereto. In the examples, "parts" and "%" indicate "parts by weight" and "% by weight" respectively.

Production Example 1 Hot water extract of Echigo grass 1000 ml of water was added to 50 g of a mixture of stem and leaves of Eki mother grass,
After extraction at 0 ° C. for 2 hours, the mixture was filtered, and the filtrate was concentrated to obtain 7.2 g of hot water extract of Masu-cho.

Production Example 2 Ethanol extract of primrose grass 1000 ml of ethanol was added to 50 g of leaves of primrose grass, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to obtain 4.1 g of ethanol extract of primrose grass.

Production Example 3 Extract of 1,3-butylene glycol from Masu-boshi To 1000 g of a mixed solution of 1,3-butylene glycol and water (1: 1) was added to 50 g of a dry matter of the whole grass of Masu-masu, and the mixture was kept at room temperature for 10 days. After extraction, the mixture was filtered, and the filtrate was concentrated.
800 g of butylene glycol extract was obtained.

[0014]

[0015]

[0016]

[0017]

[0018]

[0019]

[0020]

[0021]

[0022]

EFFECTS OF THE INVENTION The extract of the mesophyll grass of the present invention has excellent whitening properties.
The effect was shown. Next, examples will be given to explain the whitening agent of the present invention.
The effect will be described in more detail.

EXPERIMENTAL EXAMPLE 1 Melanin Production Inhibition Test Using B16 Mouse Melanoma Melanoma in the logarithmic growth phase was seeded with 20,000 cells in a φ60 mm dish, and the hot water extract of Escherichia coli was adjusted to a final concentration of 0.01% and 0.03%. Production Example 1) Eagles' MEM (containing 10% FCS) containing the same ethanol extract (Production Example 2) was added, and the mixture was cultured at 37 ° C under 5% carbon dioxide. After 5 days of culture, detach the cells from the dish, sonicate the cells,
Add 2N NaOH, treat at 60 ° C for 2 hours, and use a spectrophotometer
OD 475 nm was measured. The cell lysate after sonication was quantified for protein by Lowry's method (J. Biol. Chem., 193, 265-275, 1951), and the melanin production inhibitory effect was compared by comparing the amount of melanin per protein amount. The index was used. As is evident from the results shown in Table 1, the mesophytes exhibited a remarkable melanin production inhibitory action.

[0024]

[Table 1] Melanin production inhibitory activity 試 料 Sample Sample concentration Melanin suppression Rate (%) (μg) (%) 対 照 Control 15.1 ± 0.2 Hot water extract of mother plant (Preparation example 1) 0.01 12.4 ± 0.3 17.9 0.03 9.2 ± 0.2 39.1 Ethanol extract of mother plant herb (Production example 2) 0.01 14.2 ± 0.1 6.0 0.03 8.1 ± 0.2 46.4 ───────────メ ラ The amount of melanin in the table indicates the weight per 1mg of protein

[0025]

[0026]

[0027]

[0028]

[0029]

[0030]

[0031]

[0032]

──────────────────────────────────────────────────続 き Continuation of the front page Examiner Ikuharu Oya (56) References JP-A-5-155751 (JP, A) JP-A-3-190809 (JP, A) JP-A-10-72311 (JP, A) JP-A-10-95735 (JP, A) JP-A-6-157259 (JP, A) JP-A-62-93221 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K 7/00-7/035 A61K 7/32-7/44 A61K 7/48-7/50

Claims (2)

(57) [Claims] 1. A whitening agent comprising an extract of Masu- boshi . 2. The whitening agent according to claim 1, wherein the whitening agent contains a water-soluble solvent extract of Masu mother grass.