CN104173221A - Novel application of prunus mume extract - Google Patents
Novel application of prunus mume extract Download PDFInfo
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- CN104173221A CN104173221A CN201310193394.2A CN201310193394A CN104173221A CN 104173221 A CN104173221 A CN 104173221A CN 201310193394 A CN201310193394 A CN 201310193394A CN 104173221 A CN104173221 A CN 104173221A
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Abstract
The invention relates to a novel application of a prunus mume extract. Specifically, the invention discloses an application of the prunus mume extract as an anti-ageing effective component. Specifically, the anti-ageing effective component is an effective component capable of promoting fiber-forming cell proliferation. Experiments show that the prunus mume extract has the function of promoting the fiber-forming cell proliferation, can be made into various skin-caring cosmetic compositions and various preparations by being mixed with auxiliary materials and additives for the cosmetics, and has actual application values.
Description
Technical field
The present invention relates to the new purposes of plant extract, relate in particular to the new purposes of FLOS MUME extract.
Background technology
Fibroblast is most important cell in dermal layer of the skin, is often attached on collagen fiber, can synthesize and secrete the various kinds of cell epimatrixs such as a large amount of collagen protein, forms dermal scaffold, maintains the normal elasticity of skin and toughness.Along with people's aging, fibroblast quantity reduces, and collagen protein secretion reduces thereupon, causes the atrophy of skin middle level, shows epidermis depression, occurs the skin ageing phenomenons such as wrinkle.At present, medical cosmetology is means mainly with direct injection fibroblast, has potential safety hazard.By using some natural plant compositions, promote human body skin self fibroblast proliferation, increase collagen protein secretion, be expected to become safer, effective resisting age of skin method.
FLOS MUME is the plant Flos Mume (Prunus mume sieb.Et Zucc.) of Rosaceae Li Yake Prunus prunus mume (sieb.) sieb.et zucc. subgenus, originates in China, at existing more than 3000 cultivation history of China.Known FLOS MUME flower aspect the traditional Chinese medical science for " dispersing the stagnated live-QI to relieve the stagnation of QI, appetizing is promoted the production of body fluid, and reduces phlegm.Cure mainly stomachache due to hyperactive liver-QI attacking the stomach, uncomfortable in chest, globus hystericus, summer-heat excessive thirst, inappetence, vomiting during pregnancy, scrofula tuberculosis, varioliform exanthema." (< < Chinese medicine voluminous dictionary > > Shanghai science tech publishing house is write by Nanjing University of Traditional Chinese Medicine).The extract that discloses recently Flos Mume has effect (the Matsuda H that removes free radical, Chem Pharm Bull.2003,51 (4): 440～443), have antiallergic effect (CN102552076A), have treatment and prevention dermatitis effect (CN101461905A), there is the effect (CN1335881A that tyrosine-kinase enzyme inhibition activity check melanin generates; KR914963-B1).But, and the unexposed FLOS MUME extract of crossing has the effect that promotes fibroblast proliferation.
Summary of the invention
The object of the invention is the defect for prior art, the new purposes of FLOS MUME extract is provided.
First the present invention discloses the purposes of FLOS MUME extract as defying age effective ingredient, and described defying age effective ingredient is specially and promotes fibroblast proliferation effective ingredient effective ingredient.
Described FLOS MUME is for being derived from the alabastrum part of Rosaceae Prunus prunus mume (sieb.) sieb.et zucc. Subgenus FLOS MUME (Prunus mume sieb.Et Zucc.).
Described FLOS MUME extract is the extract that described FLOS MUME makes through extracting solvent extraction.Described extraction solvent, only otherwise damage effect of the present invention, has no particular limits, and can be water; The monohydric alcohol such as methanol, ethanol; The polyhydric alcohol such as 1,3 butylene glycol, propylene glycol; The lower alkyl esters such as ethyl acetate; Ether, acetone etc.In the present invention, also above-mentioned solvent can be used in combination.Preferred extraction solvent is water, methanol, ethanol, 1,3 butylene glycol or its combination.Especially preferred extraction solvent is water, ethanol or its combination (being ethanol water).
More specifically, FLOS MUME extract of the present invention adopts following method to make: take FLOS MUME alabastrum as raw material, crushed after being dried becomes coarse powder to sieve, get dried powder, with ethanol and/or deionized water, as extracting solvent extraction, filtrate merges, and after concentrating, obtains FLOS MUME extract.
Described concentrated method includes but not limited to: atmospheric evaporation, reduction vaporization, thin film evaporation, multiple-effect evaporation.
Preferably, when prepared by described FLOS MUME extract, meet any one in following condition or multinomial:
1) ethanol water that described extraction solvent is 50-95v% preferably, is the ethanol water of 50-90v%;
2) described FLOS MUME alabastrum is 1g:10ml-1g:50ml with the solid-liquid ratio that extracts solvent;
3) extraction time is 0.5-2 hour;
4) extract at room temperature;
When adopting 50% ethanol water to extract 1 hour with 50 times of solid-liquid ratios, can obtain higher extraction ratio.
Described FLOS MUME extract can directly be used as short fibroblast proliferation effective ingredient, or can further through conventional step purification and/or after being dried, be used as defying age effective ingredient.Wherein the method for purification includes but not limited to: the purification of saltouing, macroporous resin adsorption purification, ethanol deposition and purification, ion-exchange resin purification, flocculation sediment purification, membrane separation purification, polycaprolactam purification, silica gel adsorption chromatography purification etc., dry method includes but not limited to: oven drying method, drum-type seasoning, belt drying method, hygroscopic desiccation method, fluid-bed drying, spray drying method, hypobaric drying method, freeze-drying, infrared drying, micro-wave drying method etc.
Concrete, the purification process of described FLOS MUME extract can be: by FLOS MUME extract with after deionized water dissolving, be loaded to the absorption of macroporous adsorbent resin packed column, use successively the ethanol water eluting of deionized water, variable concentrations, collect stream part, evaporate to dryness, obtains the alcohol elution of FLOS MUME water elution position and variable concentrations.
The ethanol water concentration range of described variable concentrations is: 20-80v% ethanol water.
Further preferred, described FLOS MUME extract comprises described FLOS MUME water elution position and/or FLOS MUME 20v% alcohol elution.
Further, described purposes is: using described FLOS MUME extract as defying age effective ingredient, for the preparation of cosmetics, described defying age effective ingredient is specially and promotes fibroblast proliferation effective ingredient.
Describedly using FLOS MUME extract and refer to as defying age effective ingredient: in described cosmetic composition, can FLOS MUME extract be its unique defying age effective ingredient, or also can contain with the composition that other have senile-resistant efficacy equally FLOS MUME extract as one of its defying age effective ingredient simultaneously.
Can adopt conventional method, the FLOS MUME extract of effective dose and the conventional adjuvant of cosmetics are mixed with to various forms of cosmetics.
Each optimum condition of the present invention all can combination in any.
Test shows, FLOS MUME extract has short fibroblast proliferation and antioxidative effect, it and other used for cosmetic adjuvants and additive can be mixed and made into cosmetic composition and the various dosage form of various skin nursings.FLOS MUME extract does not have toxic and side effects to human body, has actual application value.
Accompanying drawing explanation
Fig. 1: FLOS MUME extract is to fibroblastic proliferation function result of the test
The specific embodiment
Below, by specific instantiation explanation embodiments of the present invention, those skilled in the art can understand other advantages of the present invention and effect easily by the disclosed content of this description.The present invention can also be implemented or be applied by the other different specific embodiment, and the every details in this description also can be based on different viewpoints and application, carries out various modifications or change not deviating under spirit of the present invention.
Embodiment 1 FLOS MUME extraction conditions preferably
Get FLOS MUME 10g, adopt L9 (3
3) orthogonal test (drug research common mathematical method and computer program, Guizhou science and technology publishing house, 1997) carries out the optimization of extraction process, take solid-liquid ratio (A), alcoholic solution concentration (B), extraction time (C) is experimental factor.The extraction ratio of take carries out positive quadraturing design test, Optimized Extraction Process condition as index.Factor level design is in Table 1, and wherein, from production cost consideration, extraction time is generally no more than 2 hours.The results are shown in Table 1-3.
Extracting method: by pulverizing after FLOS MUME alabastrum cleaning-drying, mix with ethanol water, at normal temperatures, extract certain hour, isolate extracting solution, after pressure reducing and steaming solvent, obtain FLOS MUME extract.According to extraction ratio=extract weight/raw material weight * 100%, calculate extraction ratio.
Table 1 orthogonal test factor level
Table 2L9 (3
3) orthogonal experiment plan takes into account result table
Table 3 orthogonal test intuitive analysis table
Intuitive analysis can obtain, and A3B2C1 is 50 times of solid-liquid ratios, 1 hour extraction time, 50% concentration of alcohol, is the optimum extraction condition of FLOS MUME extract.
Embodiment 2
By pulverizing after commercially available 100g FLOS MUME alabastrum cleaning-drying, mix with 2L70% alcoholic solution, at normal temperatures, extract 2 hours, isolate extracting solution, through decompression, steam after alcoholic solution, obtain FLOS MUME extract 13g.
Embodiment 3
By pulverizing after commercially available 50g FLOS MUME alabastrum cleaning-drying, mix with 2.5L50% alcoholic solution, at normal temperatures, extract 1 hour, isolate extracting solution, through decompression, steam after deionized water, obtain FLOS MUME extract 16g.
Embodiment 4
By pulverizing after commercially available 100g FLOS MUME alabastrum cleaning-drying, mix with 2L95% alcoholic solution, at normal temperatures, extract 2 hours, isolate extracting solution, through decompression, steam after alcoholic solution, obtain FLOS MUME extract 11g.
Embodiment 5
Get the FLOS MUME extract 10g in embodiment 2, with after deionized water dissolving, be loaded to AB-8 resin filling post, use successively deionized water, 20% ethanol, 40% ethanol, 60% ethanol and 80% ethanol elution, collect respectively eluent, decompression steams second alcohol and water, obtains respectively water elution position, 20% alcohol elution, 40% alcohol elution, 60% alcohol elution and 80% alcohol elution.
Embodiment 6
The water elution position of the FLOS MUME extract obtaining in embodiment 5,20% alcohol elution are mixed with to 10mg/mL solution with deionized water, join in fibroblast culture fluid, the deionized water sample of take not containing sample is contrast, cultivate after 48 hours, by cell dyeing, by microplate reader, measure 475nm place absorbance, assessment is to fibroblastic proliferation function.As shown in Figure 1, between blank and FLOS MUME extract, observed statistical significant difference, shown that the FLOS MUME water position of 0.1% mass concentration and 20% ethanol position of 1% mass concentration have good proliferation function to fibroblast.
Embodiment 7
The FLOS MUME extract preparing by embodiment 2 methods, with after deionized water dissolving, is mixed with 1mg/ml solution, by DPPH method, measures its oxidation resistance, with the positive contrast of VC.Result shows that 1mg/ml FLOS MUME extract DPPH clearance rate is 93.9%, and the DPPH clearance rate of positive control 5ug/ml VC is 74.0%.
The above; it is only preferred embodiment of the present invention; not to any formal and substantial restriction of the present invention; should be understood that; for those skilled in the art; do not departing under the prerequisite of the inventive method, also can make some improvement and supplement, these improvement and supplement and also should be considered as protection scope of the present invention.All those skilled in the art, without departing from the spirit and scope of the present invention, a little change of making when utilizing disclosed above technology contents, the equivalent variations of modifying and developing, be equivalent embodiment of the present invention; Meanwhile, the change of any equivalent variations that all foundations essence technology of the present invention is done above-described embodiment, modification and differentiation, all still belong in the scope of technical scheme of the present invention.
Claims (9)
1. FLOS MUME extract is as the purposes of defying age effective ingredient, and described defying age effective ingredient is specially and promotes fibroblast proliferation effective ingredient effective ingredient.
2. purposes as claimed in claim 1, is characterized in that, described FLOS MUME extract is the extract that described FLOS MUME makes through extracting solvent extraction.
3. purposes as claimed in claim 1, it is characterized in that, described FLOS MUME extract adopts following method to make: take FLOS MUME alabastrum as raw material, crushed after being dried becomes coarse powder to sieve, get dried powder, with ethanol and/or deionized water, as extracting solvent extraction, filtrate merges, and after concentrating, obtains FLOS MUME extract.
4. purposes as claimed in claim 3, is characterized in that, when prepared by described FLOS MUME extract, also meets any one in following condition or multinomial:
1) ethanol water that described extraction solvent is 50-95v%;
2) described FLOS MUME alabastrum is 1g:10ml-1g:50ml with the solid-liquid ratio that extracts solvent;
3) extraction time is 0.5-2 hour;
4) extract at room temperature.
5. purposes as claimed in claim 4, is characterized in that, described FLOS MUME extract is directly used as short fibroblast proliferation effective ingredient, or further through conventional step purification and/or after being dried, is used as defying age effective ingredient.
6. purposes as claimed in claim 5, it is characterized in that, the method of described purification is: by FLOS MUME extract with after deionized water dissolving, be loaded to the absorption of macroporous adsorbent resin packed column, use successively the ethanol water eluting of deionized water, variable concentrations, collect stream part, evaporate to dryness, obtains the alcohol elution of FLOS MUME water elution position and variable concentrations.
7. purposes as claimed in claim 6, is characterized in that, the ethanol water concentration range of described variable concentrations is: 20-80v% ethanol water.
8. purposes as claimed in claim 6, is characterized in that, described FLOS MUME extract comprises described FLOS MUME water elution position and/or FLOS MUME 20v% alcohol elution.
9. the purposes as described in claim as arbitrary in claim 1-8, it is characterized in that, described purposes is: using described FLOS MUME extract as defying age effective ingredient, for the preparation of cosmetics, described defying age effective ingredient is for promoting fibroblast proliferation effective ingredient.
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| CN201310193394.2A CN104173221A (en) | 2013-05-23 | 2013-05-23 | Novel application of prunus mume extract |
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| CN201310193394.2A CN104173221A (en) | 2013-05-23 | 2013-05-23 | Novel application of prunus mume extract |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106821851A (en) * | 2017-02-20 | 2017-06-13 | 西安科艺诗生物技术有限公司 | A kind of fresh processing method of flos mume |
| CN107802557A (en) * | 2017-12-12 | 2018-03-16 | 王露 | A kind of hand frost |
| CN107970154A (en) * | 2017-12-12 | 2018-05-01 | 王露 | A kind of shower cream with white-skinned face function |
| CN109106637A (en) * | 2018-08-28 | 2019-01-01 | 珠海欧维斯科技有限公司 | A kind of anti-oxidant and fermented plant extract to delay senescence and its preparation method and application |
| CN109303723A (en) * | 2017-07-27 | 2019-02-05 | 伽蓝（集团）股份有限公司 | Plum blossom embryo extract and its preparation method and application |
| CN109512732A (en) * | 2017-11-24 | 2019-03-26 | 北京东方淼森生物科技有限公司 | Have effects that repair exterior-applied plant extract, cosmetics of skin barrier and preparation method thereof |
| CN112451449A (en) * | 2020-12-26 | 2021-03-09 | 浙江英树生物科技有限公司 | Use of Prunus mume extract for preventing and/or repairing skin photodamage |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106821851A (en) * | 2017-02-20 | 2017-06-13 | 西安科艺诗生物技术有限公司 | A kind of fresh processing method of flos mume |
| CN106821851B (en) * | 2017-02-20 | 2019-08-02 | 西安科艺诗生物技术有限公司 | A kind of fresh processing method of flos mume |
| CN109303723A (en) * | 2017-07-27 | 2019-02-05 | 伽蓝（集团）股份有限公司 | Plum blossom embryo extract and its preparation method and application |
| CN109512732A (en) * | 2017-11-24 | 2019-03-26 | 北京东方淼森生物科技有限公司 | Have effects that repair exterior-applied plant extract, cosmetics of skin barrier and preparation method thereof |
| CN107802557A (en) * | 2017-12-12 | 2018-03-16 | 王露 | A kind of hand frost |
| CN107970154A (en) * | 2017-12-12 | 2018-05-01 | 王露 | A kind of shower cream with white-skinned face function |
| CN109106637A (en) * | 2018-08-28 | 2019-01-01 | 珠海欧维斯科技有限公司 | A kind of anti-oxidant and fermented plant extract to delay senescence and its preparation method and application |
| CN112451449A (en) * | 2020-12-26 | 2021-03-09 | 浙江英树生物科技有限公司 | Use of Prunus mume extract for preventing and/or repairing skin photodamage |
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