KR20180128773A - A composition for antioxidating, whitening and improving wrinkle comprising forsythia fruit oil or peony oil - Google Patents
A composition for antioxidating, whitening and improving wrinkle comprising forsythia fruit oil or peony oil Download PDFInfo
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- KR20180128773A KR20180128773A KR1020170064304A KR20170064304A KR20180128773A KR 20180128773 A KR20180128773 A KR 20180128773A KR 1020170064304 A KR1020170064304 A KR 1020170064304A KR 20170064304 A KR20170064304 A KR 20170064304A KR 20180128773 A KR20180128773 A KR 20180128773A
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- oil
- peony
- present
- whitening
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Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/302—Foods, ingredients or supplements having a functional effect on health having a modulating effect on age
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
Abstract
Description
본 발명은 연교 또는 작약 오일을 함유하는 조성물에 관한 것으로, 보다 구체적으로는 연교 또는 작약 오일을 유효성분으로 함유하는 항산화, 미백 및 주름 개선을 위한 화장료 조성물 및 식품 조성물에 관한 것이다. More particularly, the present invention relates to a cosmetic composition and a food composition for antioxidation, whitening and wrinkle reduction which contain a crosslinked or peony oil as an active ingredient.
사람의 피부는 각질층을 포함하는 표피와 진피, 그리고 결합조직으로 구성되어 있는데, 그 중 각질층은 표피(epidermis)의 기저세포인 케라티노사이트(keratinocyte)의 분화과정을 통해 형성되는 죽은 세포층으로 구성되어 있고 인체를 외부환경의 영향으로부터 보호해주는 역할을 담당하고 있다. 이러한 피부에서의 노화의 원인에 대해서는 크게 두 가지로 연구되고 있는데, 하나는 "내적 노화"로서 연령의 증가에 따른 피부의 구성단위인 세포의 기능변화에 기인한 것이고, 다른 하나는 "외적 노화"로서 외부 환경 즉 자외선, 공해, 스트레스 등에 의한 것으로 나눌 수 있다.The human skin consists of the epidermis, dermis, and connective tissue including the stratum corneum, and the stratum corneum is composed of a dead cell layer formed through the differentiation process of keratinocyte, the basal cell of the epidermis And is responsible for protecting the human body from the effects of the external environment. There are two major researches on the causes of skin aging. One is "internal aging", which is caused by changes in the function of cells, which are constitutive units of skin, with age, and the other is "external aging" Which can be divided into external environment such as ultraviolet rays, pollution, stress and the like.
피부의 세포는 생체 내에서 필요한 에너지 공급을 위해 인간의 생체 내에서 필요한 에너지 공급을 위해 끊임없이 일어나는 생화학 반응에서 발생하는 활성산소종에 의해 산화적 스트레스가 가해지게 되면, 세포 구성 성분들인 지질, 단백질, 탄수화물과 DNA 산화적 손상 및 효소활성을 변화시켜 피부암 등의 각종 질병을 일으키는 원인이 되며, 노화를 촉진시킨다. 아울러 외적 노화의 원인 중에서 가장 큰 부분을 차지하고 있는 것은 자외선에 의한 광노화로서 UV에 지속적인 노출을 통해 피부암 등의 급성 및 만성적인 피부의 상해가 유발된다.As the cells of the skin are exposed to oxidative stress by reactive oxygen species generated in a continuous biochemical reaction for the necessary energy supply in the human body in order to supply necessary energy in vivo, the cellular components such as lipid, protein, It changes carbohydrate and DNA oxidative damage and enzymatic activity, causing various diseases such as skin cancer, and promotes aging. In addition, the biggest cause of external aging is photoaging due to ultraviolet rays, which causes acute and chronic skin injuries such as skin cancer through continuous exposure to UV.
피부 진피층 기질을 이루는 중요한 성분의 하나인 콜라겐의 생합성과 분해는 피부 노화 억제에 핵심이 되고 있는데, 상기 활성산소종은 피부 섬유아세포에서 MMPs(matrix metalloproteinases)의 발현을 촉진하며, 자외선에 의한 콜라게나아제의 합성을 촉진시킴으로써 피부 노화를 유발한다. The biosynthesis and degradation of collagen, which is one of the important components of the dermal matrix of skin, plays a key role in inhibiting skin aging. The active oxygen species promotes the expression of matrix metalloproteinases (MMPs) in skin fibroblasts, Aging of the skin is promoted by promoting the synthesis of azelaic acid.
또한, 엘라스틴(elastin) 섬유는 콜라겐과 가교 결합을 형성하며 피부 탄력에 관여하고 있는 주름 생성에 중요한 피부 구성성분이다. 엘라스틴 섬유의 결핍과 응집, 엘라스틴 분해 효소인 엘라스타아제(elastase)의 활성도의 현격한 증가는 피부 주름생성 요인 중의 하나로 밝혀지고 있다. 엘라스타아제는 엘라스틴을 분해할 수 있는 유일한 효소로서 이에 대한 저해는 피부 주름 개선을 근본적으로 줄여줄 수 있다고 알려져 있다.In addition, elastin fibers form crosslinks with collagen, and are skin constituents that are important for wrinkle formation that is involved in skin elasticity. The deficiency and aggregation of elastin fibers and the dramatic increase in activity of the elastase, elastase, have been found to be one of the causes of skin wrinkles. Elastase is the only enzyme capable of degrading elastin, and its inhibition is known to be able to radically reduce wrinkles.
한편, 사람의 피부색은 피부 내부의 멜라닌(melanin) 농도와 분포에 따라 결정되는데, 유전적인 요인 외에도, 태양 자외선이나 피로, 스트레스 등의 환경적 또는 생리적 조건에 의해서도 영향을 받는다. 멜라닌은 피부 표피층에 있는 멜라노사이트에서 합성되는데, 멜라노사이트 내 소기관인 멜라노좀(melanosome)에서 아미노산의 일종인 티로신(tyrosine)에 티로시나아제(tyrosinase)라는 효소가 작용하여 도파(DOPA), 도파퀴논(dopaquinone)으로 바뀐 후 비효소적인 산화반응을 거쳐 만들어진다. 이와 같은 멜라닌의 합성이 피부 내에서 과도하게 일어나면, 피부 톤을 어둡게 하고, 기미, 주근깨 등을 발생시킨다. 따라서, 피부 내의 티로시나아제 활성을 저해하여 멜라닌 색소의 합성을 저해시키면, 피부 톤을 밝게 하여 피부 미백을 실현할 수 있을 뿐만 아니라 자외선, 호르몬 및 유전적인 원인에 기인하여 발생하는 기미, 주근깨 등의 피부 과색소 침착증을 개선시킬 수 있다.On the other hand, human skin color is determined by the concentration and distribution of melanin in the skin. In addition to genetic factors, skin color is also affected by environmental or physiological conditions such as sunlight, fatigue and stress. Melanin is synthesized in melanocytes in the epidermis of the skin. Melanosome, a melanosome in melanocytes, acts on tyrosine, an enzyme called amino acid tyrosinase, to form an enzyme called tyrosinase (DOPA) (dopaquinone) and then converted to non-enzymatic oxidation. When such a synthesis of melanin occurs excessively in the skin, it darkens skin tone, and causes spots and freckles. Thus, inhibition of tyrosinase activity in the skin to inhibit the synthesis of melanin pigment can lighten the skin tone and realize whitening of skin, as well as ultraviolet rays, hormones and skin caused by genetic causes, such as freckles Hypercholesterolemia can be improved.
따라서, 화장품 분야에서는 항산화, 미백 및 주름 개선효과를 가지는 기능성 화장품에 대한 연구 및 개발이 활발하게 진행되고 있으며, 특히 피부에 독성이나 자극을 주지 않기 위하여 천연 물질을 이용한 연구가 계속되고 있다.Accordingly, in the field of cosmetics, research and development on functional cosmetics having antioxidative, whitening and wrinkle-reducing effects have been actively carried out, and studies using natural substances have been continued to prevent toxicity or irritation to the skin.
한편, 연교(forsythia fruit)는 열매가 연꽃과 비슷하게 방(房)을 형성하고 있고 여러 풀 중에 길게 솟아나와(翹) 있어 얻은 이름으로, 또는 씨앗을 쪼개면 한 조각 한 조각이 깃털과 비슷하기 때문에 '잇닿다'라는 의미의 연(連)과 '깃털'이라는 의미의 교(翹)를 써서 연교라고 부른다고도 알려져 있다. 우리나라에서는 물푸레나무과의 의성개나리(Forsythia viridissima Lindley) 또는 당개나리(Forsythia suspensa Vahl)의 건조시킨 열매를 말한다. 이 약은 특이한 냄새가 있으며 그 맛은 쓰고, 기운은 약간 차다. 약효는 열을 내리고 해독하며, 풍열(風熱)을 없애고, 종기나 상처가 부은 것을 삭도록 하거나 뭉치거나 몰린것을 풀어주는 소종산결(消腫散結)하는 효능을 갖는다. 따라서 온열병 초기에 열을 내려서 고열과 정신이 혼몽한 것을 가라앉히거나 종기, 림프절염, 인후염 등에 활용할 수 있다. 사상의학에서는 소양인에게 활용할 수 있는 약재이다. 약리작용으로 항균작용, 항염증작용, 혈압강하, 지혈작용, 간치료작용, 해열, 진토, 이뇨작용이 보고되었다. 주요 화학성분으로는 포르시톨(forsythol), 스테롤(sterol) 화합물, 사포닌(saponin), 올레아놀산(oleanolic acid) 등이 있다.On the other hand, forsythia fruit is a fruit that is formed in a pool similar to that of a lotus flower, in the name of a long spring in various grasses, or because a piece of a seed is similar to a feather in a split seed, It is also known that it uses a bridge of the meaning of "come on" and a meaning of "feather" to call it an alliance. In Korea, dried fruits of Forsythia viridissima Lindley or Forsythia suspensa Vahl from Aspira. This medicine has a peculiar smell, its taste is used, its energy is a bit cold. It has the efficacy of lowering and deciphering heat, eliminating the wind heat, cutting off swelling and wounding, or dissolving or aggregating. Therefore, fever in the early stages of fever can be relieved by fever and mental congestion, constipation, lymphadenitis, sore throat can be used. In medicinal medicine, it is a medicine that can be applied to Soyangin. Antimicrobial action, anti-inflammatory action, blood pressure lowering, hemostatic action, liver treatment action, fever, swelling, diuretic action were reported by pharmacological action. Major chemical components include forsythol, sterol compounds, saponin, and oleanolic acid.
또한, 작약은 미나리아재비과에 속하는 다년생 초본식물로서 꽃이 크고 탐스러워서 함박꽃이라고도 한다. 백작약(화이트)ㅇ적작약(와인)ㅇ호작약(태백)ㅇ참작약 등 다양한 품종이 있다. 백작약은 높이 40∼50㎝로 밑부분이 비늘 같은 잎으로 싸여 있으며, 뿌리는 육질(肉質)이고 굵다. 잎은 3, 4개가 어긋나며, 3개씩 2회 갈라진다. 소엽(小葉)은 타원형 또는 도란형(倒卵形:거꿀달걀형)으로 가장자리가 밋밋하다. 꽃은 6월에 피며 백색이다.Peony is also a perennial herbaceous plant belonging to the butterfly family. There are various varieties such as peony (white) ㅇ peony (wine) ㅇ Hojo peony (Taebaek) 참 peony. It is 40 to 50 cm high and its lower part is wrapped with scaly leaves. The roots are fleshy and thick. Leaves are 3, 4 slices and divide into 2 by 3 slices. Lobules are elliptical or obovate, with flat edges. Flowers bloom in June and are white.
적작약은 뿌리가 붉은빛이 도는 품종으로 높이가 50∼80㎝이다. 뿌리는 방추형이고, 근생엽은 1∼2회 우상(羽狀)으로 갈라진다. 소엽은 피침형ㅇ타원형 또는 난형으로 가장자리가 밋밋하다. 꽃은 5∼6월에 피는데 백색ㅇ적색 등 여러 품종이 있다.It is a reddish-rooted variety with a height of 50-80cm. The roots are fusiform, and the root leaves are split one or two times in 羽 状 shape. Lobules are lanceolate o elliptical or oval, with flat edges. Flowers bloom from May to June, and there are many varieties including white o red.
한방에서는 뿌리를 약재로 사용한다. 성분은 배당체로서 파에오니플로린(paeoniflorin)과 알칼로이드인 파에오닌(paeonine)을 함유하고 탄닌ㅇ수지ㅇ안식향산도 있다. 약리적 작용으로는 흰쥐의 위장과 자궁의 평활근에 대하여 수축력을 약화시켰고, 토끼의 장관에는 진경(鎭痙) 효과를 나타내었다. 배당체 성분은 중추신경계를 억제한다. 이 밖에 항균작용이 있어서 황색포도상구균ㅇ이질균ㅇ용혈성연쇄상구균ㅇ폐렴쌍구균의 발육을 억제시킨다. 약성은 차고, 맛은 시고 쓰다. 위장염과 위장의 경련성동통에 진통효과를 나타내고, 소화장애로 복통, 설사, 복명(腹鳴)이 있을 때에 유효하며, 이질로 복통과 후중증이 있을 때에도 효과가 빠르다. 부인의 월경불순과 자궁출혈에 보혈, 진통, 통경의 효력을 나타낸다. 만성간염에도 사용되고 간장 부위의 동통에도 긴요하게 쓰인다. 또 빈혈로 인한 팔과 다리의 근육경련, 특히 배복근경련에 진경, 진통 효과가 있다. 한방에서는 많이 쓰이는 약에 속하며, 민간에서는 빈혈에 사용한다. 금기로는 산후에 발열이 심할 때에는 복용을 삼간다. 대표적인 처방으로는 작약감초탕(芍藥甘草湯)이 있다.In one room, root is used as a medicine. The ingredient contains paeoniflorin as a glycoside and paeonine as an alkaloid, and there is also tannic acid and benzoic acid. The pharmacological actions of the rats weakened the contraction of the gastrointestinal tract and uterine smooth muscle, and showed a gastric (spasmodic) effect in the intestinal tract of the rabbit. The glycoside component inhibits the central nervous system. In addition, it has antimicrobial action, so it inhibits the development of Staphylococcus aureus, Heterogeneous bacteria, hemolytic streptococci, and pneumococcal bacteria. The weakness is cold, the taste is bitter and bitter. It is effective when there is abdominal pain, diarrhea and abdominal pain due to gastroenteritis and gastrointestinal spastic pain, and it is effective even when there is abdominal pain after abdominal pain and diarrhea. It shows the effect of blood, pain, and blood pressure on the menstrual irregularity and uterine bleeding. It is also used for chronic hepatitis and is also used for the pain of the liver. In addition, muscle spasms of the arms and legs caused by anemia, especially epigastric seizures, have an effect of pain and analgesia. It belongs to medicine which is widely used in oriental medicine, and it is used for anemia in civilian. If the fever is severe in the postpartum period, do not take it. A typical prescription is peony liquorice (藥药 甘草 汤).
그러나, 아직까지 연교 및 작약의 항산화, 미백효과 및 주름개선 효과에 관해서는 보고된 바가 없다.However, the antioxidative, whitening effect and wrinkle-reducing effects of almonds and peanuts have not yet been reported.
이에 본 발명자들은 항산화, 미백효과 및 주름개선 효과를 가지면서 인체 부작용이 적은 새로운 천연물질을 개발하기 위해 계속 연구를 진행한 결과, 연교 또는 작약 오일이 세포 독성이 없으면서도 항산화, 미백효과뿐만 아니라 주름 개선효과를 나타낸다는 사실을 발견함으로써 본 발명을 완성하였다.Therefore, the inventors of the present invention have continued to develop new natural substances having antioxidative, whitening and wrinkle-reducing effects and fewer human side effects. As a result, it has been found that alcohols or peanut oil have antioxidative and whitening effects, The present invention has been completed.
따라서, 본 발명에서 해결하고자 하는 기술적 과제는 항산화, 미백효과 및 주름개선 효과를 가지면서 인체 안정성이 우수한 물질을 제공하기 위한 것이다.Therefore, a technical problem to be solved by the present invention is to provide a substance having an antioxidant, a whitening effect and a wrinkle-reducing effect while having excellent human stability.
상기한 기술적 과제를 해결하기 위하여, 본 발명에서는 연교 오일, 작약 오일 또는 이들의 혼합물을 유효성분으로 포함하는 것을 특징으로 하는 항산화, 미백 및 주름 개선을 위한 조성물을 제공한다.In order to solve the above-mentioned technical problems, the present invention provides a composition for antioxidant, whitening and wrinkle improvement, which comprises a combination oil, a peony oil or a mixture thereof as an active ingredient.
바람직하게, 본 발명에서 연교 오일, 작약 오일 또는 이들의 혼합물을 포함하는 조성물은 화장료 조성물 또는 식품 조성물일 수 있다.Preferably, in the present invention, the composition comprising a carrier oil, a peony oil or a mixture thereof may be a cosmetic composition or a food composition.
본 발명의 연교 오일 또는 작약 오일은 당업계에 공지된 일반적인 추출방법, 분리 및 정제방법을 이용하여 제조할 수 있다. 상기 추출방법으로는 유기용매를 이용하여 추출한 다음 감압증류하는 방법을 사용할 수 있으나, 이에 제한되지는 않는다.The associate oil or peanut oil of the present invention can be produced by a common extraction method, separation and purification method known in the art. As the extraction method, extraction using an organic solvent followed by vacuum distillation may be used, but the present invention is not limited thereto.
본 발명의 실시양태에 따르면, 연교 또는 작약을 2 내지 20 중량배의 n-hexane를 이용하여 3 내지 5일간 50 내지 70℃에서 추출한 다음 침적물을 여과하고 다시 동량의 핵산을 넣어 반복 추출한 후 30 내지 50℃에서 감압증류하여 핵산을 제거하여 연교 또는 작약 오일을 수득할 수 있다. According to the embodiment of the present invention, the allium or peony is extracted at 50 to 70 ° C for 3 to 5 days using 2 to 20 times by weight of n-hexane, and then the precipitate is filtered and repeatedly extracted with the same amount of nucleic acid, The nucleic acid is removed by distillation under reduced pressure at 50 캜 to obtain a bridged or peanut oil.
본 발명에서 작약의 사용부위는 잎, 꽃, 뿌리, 종자 모두 사용가능하나, 바람직한 일 양태로, 작약의 꽃을 사용하는 것을 특징으로 한다.In the present invention, the use site of the peony can be used as a leaf, a flower, a roots and a seed, but it is a preferred embodiment that a flower of a peony is used.
본 발명의 조성물에는 연교 오일, 작약 오일 또는 이들의 혼합물이 조성물의 총 중량을 기준으로 하여 0.005 내지 50 중량%, 보다 바람직하게는 0.01 내지 30 중량%, 가장 바람직하게는 0.1 내지 10 중량%로 포함할 수 있다. 이 때 연교 오일 또는 작약 오일의 함량이 0.005 중량% 미만일 경우 본 발명의 목적효과인 항산화, 미백 및 주름 개선효과를 수득할 수 없으며, 50 중량%를 초과할 경우 함량의 증가에 따라 효과가 비례적이지 않아 비효율적일 수 있으며 제형상의 안정성이 확보되지 않는 문제점이 있다.The compositions of the present invention include 0.005 to 50 wt.%, More preferably 0.01 to 30 wt.%, And most preferably 0.1 to 10 wt.%, Based on the total weight of the composition, of a carrier oil, can do. If the content of the aldehyde or peanut oil is less than 0.005 wt%, antioxidative, whitening and wrinkle reducing effects of the present invention can not be obtained. If the content is more than 50 wt%, the effect is proportionally increased There is a problem in that the stability of the shape can not be secured.
본 발명의 연교 오일, 작약 오일 또는 이들의 혼합물을 함유하는 조성물은 항산화 효과, 미백효과 및 주름 개선효과를 나타내며, 천연 물질로서 세포독성이 거의 없다. The composition of the present invention containing a perspex oil, a peony oil or a mixture thereof exhibits an antioxidative effect, a whitening effect and a wrinkle-reducing effect, and has almost no cytotoxicity as a natural substance.
본 발명의 하나의 실시양태에 따르면, 연교 오일, 작약 오일 또는 이들의 혼합물을 유효성분으로 포함하는 화장료 조성물을 제공한다.According to one embodiment of the present invention, there is provided a cosmetic composition comprising a combination oil, a peony oil or a mixture thereof as an active ingredient.
본 발명의 구체적인 하나의 실시양태에 따르면, 연교 오일을 유효성분으로 포함하는 항산화를 위한 화장료 조성물로서, 이 때 연교 오일의 농도는 바람직하게는 50 내지 800 ug/ml, 더욱 바람직하게는 400 내지 500 ug/ml의 범위이다. According to one specific embodiment of the present invention, a cosmetic composition for antioxidant comprising a carrier oil as an active ingredient, wherein the concentration of carrier oil is preferably 50 to 800 ug / ml, more preferably 400 to 500 gt; / ml < / RTI >
본 발명의 구체적인 하나의 실시양태에 따르면, 연교 오일을 유효성분으로 포함하는 미백을 위한 화장료 조성물로서, 이 때 연교 오일의 농도는 바람직하게는 50 내지 800 ug/ml, 더욱 바람직하게는 400 내지 500 ug/ml의 범위이다. According to one specific embodiment of the present invention, a cosmetic composition for whitening comprising a carrier oil as an active ingredient, wherein the concentration of the carrier oil is preferably 50 to 800 ug / ml, more preferably 400 to 500 gt; / ml < / RTI >
본 발명의 구체적인 하나의 실시양태에 따르면, 연교 오일을 유효성분으로 포함하는 주름 개선을 위한 화장료 조성물로서, 이 때 연교 오일의 농도는 바람직하게는 50 내지 800 ug/ml, 더욱 바람직하게는 400 내지 500 ug/ml의 범위이다. According to one specific embodiment of the present invention, a cosmetic composition for improving wrinkles comprising a carrier oil as an active ingredient, wherein the concentration of the carrier oil is preferably 50 to 800 ug / ml, more preferably 400 to 500 ug / 500 < / RTI > ug / ml.
본 발명의 구체적인 하나의 실시양태에 따르면, 작약 오일을 유효성분으로 포함하는 항산화를 위한 화장료 조성물로서, 이 때 작약 오일의 농도는 바람직하게는 50 내지 800 ug/ml, 더욱 바람직하게는 400 내지 500 ug/ml의 범위이다. According to one specific embodiment of the present invention, a cosmetic composition for antioxidant comprising a peony oil as an active ingredient, wherein the concentration of peony oil is preferably 50 to 800 ug / ml, more preferably 400 to 500 gt; / ml < / RTI >
본 발명의 구체적인 하나의 실시양태에 따르면, 작약 오일을 유효성분으로 포함하는 미백을 위한 화장료 조성물로서, 이 때 작약 오일의 농도는 바람직하게는 50 내지 800 ug/ml, 더욱 바람직하게는 400 내지 500 ug/ml의 범위이다. According to one specific embodiment of the present invention, a cosmetic composition for whitening comprising a peony oil as an active ingredient, wherein the concentration of peony oil is preferably 50 to 800 ug / ml, more preferably 400 to 500 gt; / ml < / RTI >
본 발명의 구체적인 하나의 실시양태에 따르면, 작약 오일을 유효성분으로 포함하는 주름 개선을 위한 화장료 조성물로서, 이 때 작약 오일의 농도는 바람직하게는 50 내지 800 ug/ml, 더욱 바람직하게는 400 내지 500 ug/ml의 범위이다. According to one specific embodiment of the present invention, a cosmetic composition for improving wrinkles comprising a peony oil as an active ingredient, wherein the concentration of peony oil is preferably 50 to 800 ug / ml, more preferably 400 to 500 ug / 500 < / RTI > ug / ml.
본 발명의 하나의 실시양태에 따른 화장료 조성물에는 유효성분으로서의 연교 오일 또는 작약 오일 이외에 화장품 조성물에 통상적으로 첨가되는 성분, 예컨대 항산화제, 안정화제, 가용화제, 비타민, 안료 및 향료와 같은 통상적인 보조제, 및 담체를 추가로 첨가할 수 있다. The cosmetic composition according to one embodiment of the present invention may contain conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances in addition to the carrier oil or peanut oil as an active ingredient, , And a carrier may be further added.
본 발명의 화장료 조성물은 당업계에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있으며, 예를 들어, 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성제-함유 클린싱, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이 등으로 제형화될 수 있으나, 이에 한정되는 것은 아니다. 보다 상세하게는, 영양 크림, 수렴 화장수, 유연 화장수, 로션, 에센스, 영양젤 또는 마사지 크림의 제형으로 제조될 수 있다.The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be prepared as a nutritional cream, a convergent lotion, a soft lotion, a lotion, an essence, a nutritional gel or a massage cream.
본 발명의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물성유, 식물성유, 왁스, 파라핀, 전분, 트라칸트 검, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the present invention is a paste, cream or gel, use is made of an animal oil, vegetable oil, wax, paraffin, starch, tragacanth gum, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .
본 발명의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the formulation of the present invention is a powder or a spray, tosse, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, chlorofluorohydrocarbons, propane / Propane or dimethyl ether.
본 발명의 제형이 용액 또는 유탁액인 경우에는 담체 성분으로서 용매, 가용화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.
본 발명의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 검등이 이용될 수 있다.In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Castellulose, aluminum metahydroxide, bentonite, agar or tracert, etc. may be used.
본 발명의 제형이 계면-활성제 함유 클린징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 라놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.
본 발명의 하나의 실시양태에 따르면, 연교 오일, 작약 오일 또는 이들의 혼합물을 유효성분으로 포함하는 식품 조성물을 제공한다.According to one embodiment of the present invention, there is provided a food composition comprising as an active ingredient a combination oil, a peony oil or a mixture thereof.
본 발명의 구체적인 하나의 실시양태에 따르면, 연교 오일을 유효성분으로 포함하는 항산화를 위한 식품 조성물로서, 이 때 연교 오일의 농도는 바람직하게는 50 내지 800 ug/ml, 더욱 바람직하게는 400 내지 500 ug/ml의 범위이다. According to one specific embodiment of the present invention, there is provided a food composition for antioxidant comprising a combination oil as an active ingredient, wherein the concentration of the combination oil is preferably 50 to 800 ug / ml, more preferably 400 to 500 gt; / ml < / RTI >
본 발명의 구체적인 하나의 실시양태에 따르면, 연교 오일을 유효성분으로 포함하는 미백을 위한 식품 조성물로서, 이 때 연교 오일의 농도는 바람직하게는 50 내지 800 ug/ml, 더욱 바람직하게는 400 내지 500 ug/ml의 범위이다. According to one specific embodiment of the present invention, a food composition for whitening comprising a carrier oil as an active ingredient, wherein the concentration of the carrier oil is preferably 50 to 800 ug / ml, more preferably 400 to 500 gt; / ml < / RTI >
본 발명의 구체적인 하나의 실시양태에 따르면, 연교 오일을 유효성분으로 포함하는 주름 개선을 위한 식품 조성물로서, 이 때 연교 오일의 농도는 바람직하게는 50 내지 800 ug/ml, 더욱 바람직하게는 400 내지 500 ug/ml의 범위이다. According to one specific embodiment of the present invention, there is provided a food composition for improving wrinkles comprising a waxy oil as an active ingredient, wherein the concentration of the waxy oil is preferably 50 to 800 ug / ml, 500 < / RTI > ug / ml.
본 발명의 구체적인 하나의 실시양태에 따르면, 작약 오일을 유효성분으로 포함하는 항산화를 위한 식품 조성물로서, 이 때 연교 오일의 농도는 바람직하게는 50 내지 800 ug/ml, 더욱 바람직하게는 400 내지 500 ug/ml의 범위이다. According to one specific embodiment of the present invention, there is provided a food composition for antioxidant comprising a peony oil as an active ingredient, wherein the concentration of the peony oil is preferably 50 to 800 ug / ml, more preferably 400 to 500 gt; / ml < / RTI >
본 발명의 구체적인 하나의 실시양태에 따르면, 작약 오일을 유효성분으로 포함하는 미백을 위한 식품 조성물로서, 이 때 연교 오일의 농도는 바람직하게는 50 내지 800 ug/ml, 더욱 바람직하게는 400 내지 500 ug/ml의 범위이다. According to one specific embodiment of the present invention, there is provided a food composition for whitening comprising peony oil as an active ingredient, wherein the concentration of peony oil is preferably 50 to 800 ug / ml, more preferably 400 to 500 gt; / ml < / RTI >
본 발명의 구체적인 하나의 실시양태에 따르면, 작약 오일을 유효성분으로 포함하는 주름 개선을 위한 식품 조성물로서, 이 때 연교 오일의 농도는 바람직하게는 50 내지 800 ug/ml, 더욱 바람직하게는 400 내지 500 ug/ml의 범위이다. According to one specific embodiment of the present invention, there is provided a food composition for improving wrinkles comprising peony oil as an active ingredient, wherein the concentration of the peony oil is preferably 50 to 800 ug / ml, 500 < / RTI > ug / ml.
또한, 본 발명의 하나의 실시양태에 따른 식품 조성물에는 유효성분으로서 연교 또는 작약 오일뿐만 아니라 식품 제조 시에 통상적으로 첨가되는 성분, 예를 들어, 단백질, 탄수화물, 지방, 영양소, 조미제 및 향미제를 추가로 포함할 수 있다.In addition, the food composition according to one embodiment of the present invention may contain, as an active ingredient, not only allied or peanut oil but also components normally added in the manufacture of food such as protein, carbohydrate, fat, nutrients, . ≪ / RTI >
상기 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어 말토스, 슈크로스, 올리고당 등; 및 폴리사카라이드, 예를 들어 덱스트린, 사이클로덱스트린 등과 같은 통상적인 당 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 향미제로서 천연 향미제 [타우마틴, 스테비아 추출물 (예를 들어 레바우디오시드 A, 글리시르히진 등]) 및 합성 향미제(사카린, 아스파르탐 등)를 사용할 수 있다.Examples of such carbohydrates are monosaccharides, such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings.
예컨대, 본 발명의 식품 조성물이 드링크제로 제조되는 경우에는 본 발명의 연교 또는 작약 오일 이외에 구연산, 액상과당, 설탕, 포도당, 초산, 사과산, 과즙, 두충 추출액, 대추 추출액, 감초 추출액 등을 추가로 포함시킬 수 있다.For example, when the food composition of the present invention is prepared as a drink, it may further contain citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, mulberry extract, jujube extract, licorice extract, etc., .
한편, 본 발명의 연교 오일 또는 작약 오일은 천연물질로서 인체에 무해하며, 독성 및 부작용이 거의 없으므로 장기간 사용시에도 안심하고 사용할 수 있으며, 특히 상기한 바와 같은 화장료, 약학적 및 식품 조성물에 안전하게 적용할 수 있다.Meanwhile, the artificial oil or peanut oil of the present invention is a natural substance which is harmless to the human body and has little toxicity and side effects. Therefore, it can be safely used for a long period of use and can be safely applied to the cosmetic, .
이와 같이, 본 발명의 연교 오일 또는 작약 오일을 유효성분으로 포함하는 조성물은 DPPH 및 ABTS 자유 라디칼 소거활성이 우수하여 항산화 효과가 있으며, DOPA 산화 저해활성을 가짐으로써 미백효과를 가질 뿐만 아니라 엘라스타아제 및 콜라게나아제 활성을 저해시킴으로써 주름 개선효과를 가진다. 또한 본 발명의 연교 또는 작약 오일은 세포 독성 및 피부 부작용이 없어 화장료 조성물 및 식품 조성물에 안전하게 사용할 수 있다. As described above, the composition comprising the associate oil or peanut oil of the present invention as an active ingredient is excellent in DPPH and ABTS free radical scavenging activity and has an antioxidative effect and has a DOPA oxidation inhibitory activity, thereby having a whitening effect, And collagenase activity, thereby having a wrinkle-reducing effect. In addition, the linkage or peony oil of the present invention is free from cytotoxicity and skin side effects, and can be safely used in cosmetic compositions and food compositions.
도 1은 본 발명의 연교 오일 및 작약 오일의 DPPH 자유 라디칼 소거활성 측정결과를 나타낸 그래프이다.
도 2는 본 발명의 연교 오일 및 작약 오일의 ABTS 라디칼 소거능을 나타낸 그래프이다.
도 3은 본 발명의 연교 및 작약 오일의 DOPA 산화 저해 활성억제 효과를 나타낸 그래프이다.
도 4는 본 발명의 연교 오일 및 작약 오일의 엘라스타아제 저해활성 측정결과를 나타낸 그래프이다.
도 5는 본 발명의 연교 오일 및 작약 오일의 콜라게나아제 저해 활성을 나타낸 그래프이다. 1 is a graph showing the results of measurement of DPPH free radical scavenging activity of persic oil and peanut oil of the present invention.
FIG. 2 is a graph showing the ABTS radical scavenging ability of the associate oil and peanut oil of the present invention.
FIG. 3 is a graph showing the effect of the present invention on inhibiting DOPA oxidation inhibitory activity of a linkage and peony oil.
4 is a graph showing the results of measuring the elastase inhibitory activity of the persic oil and peanut oil of the present invention.
FIG. 5 is a graph showing the collagenase inhibitory activity of the persic oil and peanut oil of the present invention.
이하, 본 발명의 이해를 돕기 위하여 실시예 등을 들어 상세하게 설명하기로 한다. 그러나, 본 발명에 따른 실시예들은 여러 가지 다른 형태로 변형될 수 있으며, 본 발명의 범위가 하기 실시예들에 한정되는 것으로 해석되어서는 안 된다. 본 발명의 실시예들은 당업계에서 평균적인 지식을 가진 자에게 본 발명을 보다 완전하게 설명하기 위해 제공되는 것이다. Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.
<실시예 1> 연교 및 작약 오일의 제조≪ Example 1 > Manufacture of allium and peony oil
연교 및 작약(화이트, 와인, 태백) 각각에 n-hexane 10 중량배의 양으로 첨가하여 4일간 60℃에서 추출하였다. 침적물을 여과하고 다시 동량의 핵산을 넣어 3회 반복 추출하였다. 추출물을 40℃에서 감압증류하여 핵산을 제거하였다. 추출한 성분은 콘크리트라고 하며, 지방산과 왁스류, 색소 등이 포함되어 있어 반고체 상태로 어두운 색상을 지닌다. 콘크리트를 원심분리기를 이용하여 15℃, 1H 15,000rpm 으로 분리하여 앱솔루트 오일을 제조하였다. (White, wine, Taebaek) in an amount of 10 times by weight of n-hexane and extracted at 60 캜 for 4 days. The precipitate was filtered, and the same amount of nucleic acid was added thereto and repeatedly extracted three times. The extract was subjected to vacuum distillation at 40 ° C to remove the nucleic acid. The extract is called concrete, and contains a fatty acid, wax, pigment, etc., and it has a semi-solid state and a dark color. Absolute oil was prepared by separating the concrete at 15 ℃ and 1H 15,000 rpm using a centrifuge.
<시험예 1> 항산화 효과 측정<Test Example 1> Measurement of antioxidant effect
(1) DPPH 자유 라디칼 소거활성 측정(1) Measurement of DPPH free radical scavenging activity
전자공여능(EDA; electron donating ability)은 Blois의 방법(1958)에 따라 측정하였다. 각 시료용액 2 ml에 0.2 mM의 1,1-diphenyl-2-picrylhydrazyl(DPPH) 1 ml 넣고 교반한 후 30분간 방치한 다음 517 nm에서 흡광도를 측정하였다. 전자공여 능은 시료용액의 첨가구와 무 첨가군의 흡광도 감소율로 나타내었다. The electron donating ability (EDA) was measured according to Blois's method (1958). 1 ml of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) was added to 2 ml of each sample solution. After stirring for 30 minutes, absorbance was measured at 517 nm. The electron donating ability was expressed by the absorbance reduction rate of the sample solution added group and the no added group.
전자공여능 측정에 사용된 1,1-diphenyl-2-picryl-hydrazyl(DPPH)는 화학적으로 안정화된 free radical을 가지고 있는 물질로 유기용매에 매우 안정하며 515~520nm 부근에서 최대 흡광도를 가지는 보라색 화합물이다. 항산화활성이 있는 물질과 만나면 전자를 내어주면서 radical이 소멸되고 탈색이 되기 때문에 항산화 활성을 육안으로 쉽게 관찰할 수 있는 장점이 있어 다양한 소재로부터 항산화 물질을 검색하는데 많이 이용되고 있다. DPPH로 먼저 산화를 개시한 후 각 시료에 의해서 free radical에 대한 소거 활성 측정 능력을 흡광도로 확인하였다. 환원시키거나 상쇄시키는 능력이 크면 높은 라디칼에 대한 소거 활성을 기대할 수 있으며, 인체 내에서 free radical에 의한 노화를 억제하는 척도로도 이용할 수 있다. 1,1-diphenyl-2-picryl-hydrazyl (DPPH), which is used for the determination of electron donating ability, is a substance having chemically stabilized free radicals and is a very stable organic solvent and a purple compound having maximum absorbance in the vicinity of 515 to 520 nm . The antioxidant activity of the antioxidant can be easily observed by the naked eye because the radical is destroyed and decolorized when the antioxidant is contacted with the substance having the antioxidant activity. After initial oxidation with DPPH, the ability to measure scavenging activity of free radicals was confirmed by absorbance. If the ability to reduce or offset is high, the scavenging activity against high radicals can be expected, and it can also be used as a measure to inhibit free radical scavenging in the human body.
도 1은 본 발명의 연교 및 작약 오일의 DPPH 자유 라디칼 소거활성 측정결과를 나타낸 그래프이다. 여기에서 FH는 연교 오일이고, PW는 작약 화이트 오일이고, PT는 작약 태백 오일이고, PWW는 작약 와인 오일이다. 도 1에서 보듯이, DPPH 자유 라디칼 소거 효과는 연교 및 작약 오일의 농도에 의존적임을 확인하였고, 특히 연교 오일은 500 ug/ml에서는 23.34%의 항산화 효과를 나타내었으며, 작약 오일은 태백이 93.95%로 이는 대조군으로 사용한 BHA의 94.05%와 유사한 항산화 효과를 나타내었다. 이는 작약 오일이 자유라디칼을 환원시키거나 상쇄시키는 능력을 수행하여 기능성화장품에 적용하였을 때 자유 라디칼에 의한 노화를 억제하는 역할을 수행할 수 있을 것이라 판단하였다.BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the results of measurement of DPPH free radical scavenging activity of the linkage and peanut oil of the present invention. FIG. Here, FH is a combination oil, PW is a peony oil, PT is a peony oil, and PWW is a peony wine oil. As shown in FIG. 1, DPPH free radical scavenging effect was found to depend on the concentration of persicolinate and peanut oil. In particular, the peanut oil showed 23.34% antioxidative effect at 500 ug / ml, and the peanut oil was 93.95% This showed similar antioxidative effect to that of BHA used as a control group. It was concluded that peony oil could play a role of suppressing free radical aging when applied to functional cosmetics by performing the ability to reduce or offset free radicals.
(2) ABTS radical cation 소거능 측정 (2) ABTS radical cation scavenging ability measurement
ABTS radical을 이용한 항산화력 측정은 ABTS+ cation decolorization assay 방법(Roberta, et al., 1999)에 의하여 측정하였다. 7 mM 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)와 2.4 mM potassium persulfate를 혼합하여 실온인 암소에서 24시간 동안 방치하여 ABTS+을 형성시킨 후 ethanol로 희석하여 ABTS+100 ul에 시료 100 ul를 가하여 1분 동안 방치한 후 732 nm에서 흡광도를 측정하였다.Antioxidant activity using ABTS radical was measured by ABTS + cation decolorization assay (Roberta, et al., 1999). After incubation at room temperature for 24 hours, ABTS + was formed, and the cells were diluted with ethanol to obtain ABTS + 100 μl. 100 μl of the sample was added and left for 1 minute, and the absorbance was measured at 732 nm.
DPPH radical의 소거활성과 같이 일반적으로 많이 사용되는 ABTS radical cation 소거능은 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt(ABTS)와potassium persulfate와의 반응으로 ABTS+· radical이 생성되면 특유의 색인 청록색을 띄게 되는데, 시료를 첨가함에 따라 연한 녹색으로decolorization되는 것을 측정하는 방법이며, hydrogen-donating antioxidant와 chain breaking antioxidant 모두를 측정할 수 있다. ABTS radical radical scavenging activity such as DPPH radical scavenging activity is generally observed in the reaction of potassium persulfate with 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) When it is generated, it becomes a specific cyan color. It is a method to measure decolorization by light green as a sample is added. It can measure both hydrogen-donating antioxidant and chain breaking antioxidant.
도 2는 본 발명의 연교 오일 및 작약 오일의 ABTS 라디칼 소거능을 나타낸 그래프이다. 여기에서 FH는 연교 오일이고, PW는 작약 화이트 오일이고, PT는 작약 태백 오일이고, PWW는 작약 와인 오일이다. 도 2에서 보듯이, ABTS radical cation 소거능을 측정한 결과 500 ug/ml의 농도에서 연교, 화이트 태백, 와인 순으로 75.03%, 79.29%, 79.57%, 63.36%의 활성을 나타냈으며, 이는 대조군인 BHA와 비교해서도 더 우수한 결과를 나타내었다. FIG. 2 is a graph showing the ABTS radical scavenging ability of the associate oil and peanut oil of the present invention. Here, FH is a combination oil, PW is a peony oil, PT is a peony oil, and PWW is a peony wine oil. As shown in FIG. 2, the ABTS radical cation scavenging activity was 75.03%, 79.29%, 79.57%, and 63.36% in the order of Kwangyo, White Taebaek and Wine at the concentration of 500 ug / ml, The results were even better.
<시험예 2> 미백 활성 측정<Test Example 2> Measurement of whitening activity
미백 활성은 DOPA 산화 저해 활성으로 측정하였다. 측정은 Yagi 등의 방법에 따라 측정하였다. 반응구는 1/15M sodium phosphate buffer (pH 6.8) 0.5mL에 10mM L-DOPA을 녹인 기질액 0.2mL및 시료용액 0.1mL의 혼합액에 mushroom tyrosinase (110U/mL) 0.2mL 첨가하여 25℃에서 2분간 반응시켜 반응액 중에 생성된 DOPA chrome을 475nm에서 측정하였다. DOPA 산화 저해 활성 측정은 시료용액의 첨가구와 무 첨가구의 흡광도 감소율로 나타내었다. 양성대조군으로 알부틴(Arbutine)을 사용하였다.The whitening activity was measured by DOPA oxidation inhibitory activity. The measurement was carried out according to the method of Yagi et al. To the reaction mixture, 0.2 mL of mushroom tyrosinase (110 U / mL) was added to a mixture of 0.2 mL of the substrate solution in which 10 mM L-DOPA had been dissolved and 0.5 mL of the sample solution in 0.5 mL of 1 / 15M sodium phosphate buffer (pH 6.8) And the DOPA chrome produced in the reaction solution was measured at 475 nm. The DOPA oxidation inhibitory activity was measured by the absorbance reduction ratio of the sample solution with and without the sample solution. Arbutine was used as a positive control.
도 3은 본 발명의 연교 및 작약 오일의 DOPA 산화 저해 활성억제 효과를 나타낸 그래프이다. 여기에서, FH는 연교 오일이고, PW는 작약 화이트 오일이고, PT는 작약 태백 오일이고, PWW는 작약 와인 오일이다. 도 3에서 보듯이, 연교오일은 500 ug/ml 농도에서 93.73%의 저해활성을 나타내었으며, 작약 오일은 와인, 태백, 화이트 순으로 83.40%, 61.26%, 42.21%의 활성을 나타내었다. FIG. 3 is a graph showing the effect of the present invention on inhibiting DOPA oxidation inhibitory activity of a linking oil and peony oil. Here, FH is associate oil, PW is Peony white oil, PT is Peony Tae Baek Oil, and PWW is Peony Wine Oil. As shown in FIG. 3, the inhibitory activity of almond oil was 93.73% at the concentration of 500 ug / ml, and that of the peony oil was 83.40%, 61.26% and 42.21% in the order of wine, Taebaek and white.
<시험예 3> 주름개선 효과 측정Test Example 3 Measurement of wrinkle improvement effect
(1) 엘라스타아제(Elastase) 저해 활성 측정(1) Measurement of Elastase Inhibitory Activity
자외선 및 활성산소 등에 의해 유발되어 피부 진피층에 존재하는 matrix-metalloprotein ases (MMPs)는 피부노화, 특히 주름생성과 밀접한 관계가 있다. MMPs를 이루는 주요성분으로는 콜라게나아제(collagenase), 젤라티나아제(gelatinase) 및 엘라스타아제(elastase) 등이 있으며, 자외선과 같은 내ㅇ외적 스트레스로 인하여 탄력성, 윤택성이 감소하고 과다 발현된 엘라스타아제에 의하여 elastin의 그물망 구조가 깨지게 되면 피부가 처지고 주름이 생기므로 피부의 탄력감소 및 주름생성에 있어서 엘라스타아제의 활성 감소는 매우 중요하다. Matrix-metalloprotein ases (MMPs) present in the dermal layer of skin induced by ultraviolet and reactive oxygen species are closely related to skin aging, especially wrinkle formation. The main constituents of MMPs are collagenase, gelatinase and elastase, and they are reduced in elasticity and elasticity due to external stresses such as ultraviolet rays, It is very important to reduce the elasticity of the skin and reduce the activity of the elastase in wrinkle formation because the skin is sagged and wrinkled when the network structure of the elastin is broken by the starase.
엘라스타아제 저해활성 측정은 다음과 같이 측정하였다. 시료를 일정 농도가 되도록 조제하여 0.5 ml씩 시험관에 취하고, 50 mM tris-HCl buffer (pH 8.6)에 녹인 porcine pancreas elastase (2.5 Unit/ml)용액 0.5 ml을 가한 후 50 mM tris-HCl buffer(pH 8.6)에 녹인 기질 N-succinyl-(L-Ala)3-p-nitroanilide (0.5mg/ml)을 첨가하여 20분간 반응시켜 기질로부터 생성되는 p-nitroanilide의 생성량을 405 nm에서 측정하였다. Elastase 저해 활성은 시료용액의 첨가구와 무첨가군의 흡광도 감소율로 나타내었다. 양성대조군으로 우르솔산(Ursolic acid, UA)을 사용하였다. Elastase inhibitory activity was measured as follows. 0.5 ml of porcine pancreas elastase (2.5 Unit / ml) dissolved in 50 mM tris-HCl buffer (pH 8.6) was added to each well and the mixture was added to 50 mM tris-HCl buffer N-succinyl- (L-Ala) 3-p-nitroanilide (0.5 mg / ml) was added to the reaction solution for 20 minutes to measure the amount of p-nitroanilide produced from the substrate at 405 nm. Elastase inhibitory activity was expressed as the absorbance reduction rate of the sample solution with or without the addition of the sample solution. Ursolic acid (UA) was used as a positive control.
도 4는 본 발명의 연교 오일 및 작약 오일의 엘라스타아제 저해활성 측정결과를 나타낸 그래프이다. 여기에서 FH는 연교 오일이고, PW는 작약 화이트 오일이고, PT는 작약 태백 오일이고, PWW는 작약 와인 오일이다. 도 4에서 보듯이, 연교 오일은 제외하고는 엘라스타아제 활성 저해 효과 없는 것으로 나타났으며 연교 오일은 500 ug/ml 농도에서 44.33%의 엘라스타아제 활성 억제효과를 나타내었다. 4 is a graph showing the results of measuring the elastase inhibitory activity of the persic oil and peanut oil of the present invention. Here, FH is a combination oil, PW is a peony oil, PT is a peony oil, and PWW is a peony wine oil. As shown in FIG. 4, it was found that the inhibitory effect of elastase activity was not observed except for the sustained-release oil, and the sustained-release oil exhibited an inhibitory effect of the elastase activity of 44.33% at the concentration of 500 ug / ml.
(2) 콜라게나아제(Collagenase) 저해활성 측정(2) Measurement of inhibitory activity of collagenase
콜라게나아제 저해활성 측정은 다음과 같이 측정하였다. 즉 반응구는 0.1 M tris-HCl buffer (pH 7.5)에 4 mM CaCl2를 첨가하여, 4-phenylazobenzyl oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (0.3 mg/mL)를 녹인 기질액 0.25 mL 및 시료용액 0.1 mL의 혼합액에 콜라게나아제 (0.2 mg/mL) 0.15 mL를 첨가하여 실온에서 20분간 정치한 후 6% citric acid 0.5 mL을 넣어 반응을 정지 시킨 후, ethyl acetate 1.5 mL을 첨가하여 320 nm에서 흡광도를 측정하였다. 콜라게나아제 저해활성은 시료용액의 첨가구와 무 첨가구의 흡광도 감소율로 나타내었다. The collagenase inhibitory activity was measured as follows. In other words, the reaction mixture was added with 0.25 mL of a substrate solution in which 4 mM CaCl 2 was added to 0.1 M tris-HCl buffer (pH 7.5) to dissolve 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro- And 0.1 mL of sample solution, add 0.15 mL of collagenase (0.2 mg / mL), allow to stand for 20 minutes at room temperature, add 0.5 mL of 6% citric acid to stop the reaction, add 1.5 mL of ethyl acetate Absorbance was measured at 320 nm. The collagenase inhibitory activity was expressed by the absorbance reduction ratio of the sample solution added and the no-added solution.
콜라게나아제는 결합조직의 탄력을 나타내며 진피층의 90%이상을 구성하고 피부의 주름과 보습 기능을 갖고 있으며, 나이가 들면서 생합성이 감소하는 대표적인 물질이다. 콜라겐을 분해하는 효소는 그 종류가 다양하며 가장 많이 알려져 있는 것이 콜라게나아제이다. 양성대조군으로 우르솔산(Ursolic acid, UA)을 사용하였다. Collagenase represents the elasticity of connective tissues and constitutes more than 90% of the dermis. It has wrinkles and moisturizing function of the skin, and is a typical material in which the biosynthesis decreases with age. The enzyme that decomposes collagen varies in its kind, and the most known is collagenase. Ursolic acid (UA) was used as a positive control.
도 5는 본 발명의 연교 오일 및 작약 오일의 콜라게나아제 저해 활성을 나타낸 그래프이다. 여기에서, FH는 연교 오일이고, PW는 작약 화이트 오일이고, PT는 작약 태백 오일이고, PWW는 작약 와인 오일이다. 도 5에서 보듯이, 콜라게나아제 활성은 100 ug/ml 농도에서 연교, 작약화이트, 작약태백, 작약와인 순으로 71.03%, 64.27%, 48.31%, 73.99% 저해율을 나타내었으며, 500 ug/ml 농도에서 연교, 작약화이트, 작약태백, 작약와인 순으로 76.85%, 63.21%, 60.89%, 74.74% 저해율을 나타내어 작약화이트와 작약와인은 100ug/ml, 500 ug/ml 증가시에도 유사한 저해율을 나타내는 것으로 나타났다.FIG. 5 is a graph showing the collagenase inhibitory activity of the persic oil and peanut oil of the present invention. Here, FH is associate oil, PW is Peony white oil, PT is Peony Tae Baek Oil, and PWW is Peony Wine Oil. As shown in FIG. 5, the collagenase activity showed inhibition rates of 71.03%, 64.27%, 48.31%, and 73.99% at 100 ug / ml concentration, , 75.85%, 63.81%, 60.89% and 74.74%, respectively, in the order of white pear, white peony, white peony, and peony wine. .
이와 같이, 본 발명의 연교 오일 또는 작약 오일을 유효성분으로 포함하는 조성물은 DPPH 및 ABTS 자유 라디칼 소거활성이 우수하여 항산화 효과가 있으며, DOPA 산화 저해활성을 가짐으로써 미백효과를 가질 뿐만 아니라 엘라스타아제 및 콜라게나아제 활성을 저해시킴으로써 주름 개선효과를 가진다. 또한 본 발명의 연교 또는 작약 오일은 세포 독성 및 피부 부작용이 없어 화장료 조성물 및 식품 조성물에 안전하게 사용할 수 있다. As described above, the composition comprising the associate oil or peanut oil of the present invention as an active ingredient is excellent in DPPH and ABTS free radical scavenging activity and has an antioxidative effect and has a DOPA oxidation inhibitory activity, thereby having a whitening effect, And collagenase activity, thereby having a wrinkle-reducing effect. In addition, the linkage or peony oil of the present invention is free from cytotoxicity and skin side effects, and can be safely used in cosmetic compositions and food compositions.
Claims (4)
상기 연교 오일, 작약 오일 또는 이들의 혼합물이 조성물 총 중량을 기준으로 하여 0.005 내지 50 중량%의 양으로 포함된 것을 특징으로 하는 화장료 조성물.The method according to claim 1,
The cosmetic composition is characterized in that it is contained in an amount of 0.005 to 50% by weight based on the total weight of the composition.
상기 연교 오일, 작약 오일 또는 이들의 혼합물이 조성물 총 중량을 기준으로 하여 0.005 내지 50 중량%의 양으로 포함된 것을 특징으로 하는 식품 조성물.3. The method of claim 2,
Characterized in that it is contained in an amount of from 0.005 to 50% by weight, based on the total weight of the composition.
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CN109430659A (en) * | 2018-12-25 | 2019-03-08 | 哈尔滨氧态健康科技股份有限公司 | A kind of peony seed oil cream and preparation method thereof |
CN114886942A (en) * | 2022-05-19 | 2022-08-12 | 云南西草资源开发有限公司 | Hemostatic composition and application thereof |
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CN109430659A (en) * | 2018-12-25 | 2019-03-08 | 哈尔滨氧态健康科技股份有限公司 | A kind of peony seed oil cream and preparation method thereof |
CN114886942A (en) * | 2022-05-19 | 2022-08-12 | 云南西草资源开发有限公司 | Hemostatic composition and application thereof |
CN114886942B (en) * | 2022-05-19 | 2023-11-21 | 云南西草资源开发有限公司 | Hemostatic composition and application thereof |
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