CN103860611A - Crape myrtle extract as well as extraction method and application thereof - Google Patents
Crape myrtle extract as well as extraction method and application thereof Download PDFInfo
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- CN103860611A CN103860611A CN201410019586.6A CN201410019586A CN103860611A CN 103860611 A CN103860611 A CN 103860611A CN 201410019586 A CN201410019586 A CN 201410019586A CN 103860611 A CN103860611 A CN 103860611A
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Abstract
The invention belongs to the technical field of plant extraction and particularly relates to crape myrtle extract as well as an extraction method and an application thereof. The crape myrtle extract is the part of petroleum ether and/or the part of ethyl acetate and/or the part of n-butyl alcohol of flowers of lythraceae crape-myrtle plants. The crape myrtle extract has good effects of inhibiting the activity of alpha-glucosaccharase and reducing the blood sugar.
Description
technical field
The invention belongs to technical field of plant extraction, be specifically related to a kind of Lagerstroemia indica L. extract and extracting method thereof, application.
background technology
Lagerstroemia
lagerstroemiabelong to Myrtales Lythraceae, Lythraceae plant of Lagerstroemia is abundant in china natural resources, but only as ornamental plant cultivation, domestic less to its research report.Chemical constitution study shows, the report of plant of Lagerstroemia chemical composition is first appeared in nineteen forty-two, so far leaf, fruit or the stem of more than 10 kind of plant to this genus are studied, find that this platymiscium chemical composition type is various, mainly comprise tannin class, ellagic acid class, terpenoid, alkaloids, flavonoid, lignanoids, coumarin and anthraquinone etc.At present, this platymiscium of research report mainly concentrates on Lagerstroemia indica L.
lagerstroemia indical., southern Lagerstroemia indica L.
l. subcostata, Flos Caryophylli Lagerstroemia indica L.
l. speciosa, Wu Jiu island Lagerstroemia indica L.
l. fauriei, Guilin Lagerstroemia indica L.
l. guilinensis, fine hair Lagerstroemia indica L.
l. tomentosa, lobule Lagerstroemia indica L.
l. parviflora.Pharmacological research shows, this genus various plants as conventional medicament, has multiple biological activity among the people, as blood sugar lowering, cough-relieving, convergence antiinflammatory, antioxidation, the effect such as antibacterial.As be grown in Filipine
l. speciosaits leaf is widely used in treatment diabetes and nephropathy, its blood sugar reducing component has contrary gallotannin acids chemicals, and as PGG, valoneaic acid dilactone, corosolic acid (CA), it may be by strengthening sensitivity, the inhibition of insulin
α-amylase and
α-glycosidase activity or minimizing gluconeogenesis, promote glycolysis to bring into play hypoglycemic activity;
l. parviflorause at India's Chang Zuowei antitussive among the people and astringency.
Lagerstroemia indica L. (
lagerstroemia indical.), have another name called all round victory, the tree that itches, cherry-apple tree, Lagerstroemia indica L. etc., for Lythraceae Lagerstroemia machaka or dungarunga, be distributed widely in China south China, Central China, East China, North China and southwestern all provinces, the bright-coloured beauty of pattern, the florescence is long, life-span is long, the age of tree has and reaches 200 years, and extensively cultivate as viewing and admiring tree in flower garden existing torrid areas, sometimes also makes potted landscape.Floral white be silver-colored common vetch (
lagerstroemia indical. f. alba (Nichols.) Rehd), the timber of Lagerstroemia indica L. is hard, corrosion resistant, can make the material such as farm implements, furniture, building; Bark, leaf and flower are strong cathartic; Root and bark decocting liquid can be controlled spitting of blood, spit blood, have blood in stool.
Chemical research shows, from Lagerstroemia indica L., contain separate obtain 3,3'-4-tri--
o-methyl ellagic acid, agerstroemine, lagerine, ten tooth grass time alkali, 5-epidihydrolyfoline, dihydrolyfoline, delphinidin-3-arabinoside, the compositions such as ellagic acid class, alkaloids, flavonoid, aromatic acid such as petunidin-3-arabinoside, malvidin-3-arabinoside.Lagerstroemia indica L.
l. indicaflower can manage property after chuonic metrorrhagia, obstructed, indefinite, metrorrhagia leukorrhagia, drench drop, wash scabies leprosy Xian skin ulcer, also have in addition resisiting influenza virus, antifibrin-ferment active function, fracture, mastitis, cirrhotic ascites are also had to certain effect.
At present, although have a large amount of bibliographical informations about Flos Caryophylli Lagerstroemia indica L.
l. speciosathe active component of hypoglycemic activity and possible mechanism of action, still, same platymiscium chemical composition has certain difference, just may bring different pharmacological actions.Exist about Lagerstroemia indica L. at present
αthe research of-glucosidase inhibitor, diabetes aspect has no report, need to further further investigate, and develops its practical application aspect Remedies for diabetes.
summary of the invention
The object of the present invention is to provide a kind of Lagerstroemia indica L. extract with hypoglycemic activity and extracting method thereof, application.
The present invention is by the following technical solutions:
A kind of Lagerstroemia indica L. extract is petroleum ether part and/or ethyl acetate extract and/or the n-butanol portion of the flower of Lythraceae plant of Lagerstroemia.
Petroleum ether part, ethyl acetate extract and n-butanol portion are to be raw material by the flower of Lythraceae plant of Lagerstroemia, first obtain total ethanol extractum with ethanol extraction, again total ethanol extractum is scattered in water, extract with petroleum ether, ethyl acetate, n-butyl alcohol, the petroleum ether extract, acetic acid ethyl ester extract and the n-butyl alcohol extract that obtain are respectively petroleum ether part, ethyl acetate extract and n-butanol portion.
Described Lythraceae plant of Lagerstroemia is: Lagerstroemia indica L.
lagerstroemia indicaor silver-colored common vetch L.
lagerstroemia indical. f. alba (Nichols.) Rehd.
The acquisition by the following method of described total ethanol extractum: Lythraceae plant of Lagerstroemia spend first drying, pulverizing, then use ethanol (60 ~ 80%) to soak (0.5-2h), heating and refluxing extraction 2-3 time, 0.5-2h at every turn, merge extractive liquid,, filtration, the concentrated total ethanol extractum that to obtain.
The extracting method of Lagerstroemia indica L. extract, Lythraceae plant of Lagerstroemia spend first drying, pulverizing, then use ethanol (60 ~ 80%) to soak (0.5-2h), heating and refluxing extraction 2-3 time, each 0.5-2h, merge extractive liquid,, filtration, concentratedly to obtain total ethanol extractum, then total ethanol extractum is scattered in water, extract with petroleum ether, ethyl acetate, n-butyl alcohol, the petroleum ether extract, acetic acid ethyl ester extract and the n-butyl alcohol extract that obtain are Lagerstroemia indica L. extract.
Lagerstroemia indica L. extract is in preparation
αapplication in-glucosidase inhibitor.
The application of Lagerstroemia indica L. extract aspect blood sugar lowering.
Lagerstroemia indica L. extract is in the application of preparing aspect hypoglycemic drug.
The application of Lagerstroemia indica L. extract aspect preparation treatment diabetes medicament.
Lagerstroemia indica L. extract of the present invention has well
α-Glucosidase inhibitor activity, in vivo test confirms, there is certain therapeutic effect at the EA position of Flos lagerstroemiae indicae and silver-colored common vetch flower and BU position to the diabetic mice of alloxan induction, its mechanism of action may be relevant with the approach such as synthetic, the decomposition that reduces liver glycogen of its promotion liver glycogen, reduce the content of TC and TG in serum, correct the lipid metabolic disorder that diabetes cause; Reduce the content of MDA in serum, increased SOD level, strengthens the anti-oxidation function of diabetic mice body, and protection body is avoided the further injury of free radical, and then it is relevant to reach the effect for the treatment of diabetes.
accompanying drawing explanation
Fig. 1 is Lagerstroemia indica L. the different extracted parts, mass concentration pair
αthe curve chart of-Glucosidase inhibitor activity influence;
Fig. 2 is silver-colored common vetch the different extracted parts, mass concentration pair
αthe curve chart of-Glucosidase inhibitor activity influence.
the specific embodiment
The preparation of embodiment 1 Lagerstroemia indica L. extract
Flos lagerstroemiae indicae natural drying, pulverizing, with certain density ethanol (70V%) immersion 2h, reflux 2 times, each 1 h, merge, filter, concentrate to obtain Flos lagerstroemiae indicae total ethanol extractum, total extractum is scattered in water, extracts successively with petroleum ether, ethyl acetate, n-butyl alcohol, obtains respectively Flos lagerstroemiae indicae petroleum ether (PE) position, ethyl acetate (EA) position and n-butyl alcohol (BU) position.
The preparation of embodiment 2 silver medal common vetch extracts
Silver common vetch flower natural drying, pulverizing, with certain density ethanol (70V%) immersion 1h, reflux 2 times, each 1 h, merge, filter, concentrate and to obtain silver-colored common vetch flower total ethanol extractum, total extractum is scattered in water, extract with petroleum ether, ethyl acetate, n-butyl alcohol successively, respectively silver-colored common vetch colored petroleum ether part, ethyl acetate extract and n-butanol portion.
The preparation of embodiment 3 Lagerstroemia indica L. extracts
As different from Example 1: concentration of alcohol is 60V%, soak 0.5h, reflux 3 times, each 0.5h.
The preparation of embodiment 4 Lagerstroemia indica L. extracts
As different from Example 1: concentration of alcohol is 80V%, soak 1h, reflux 2 times, each 2h.
The preparation of embodiment 5 silver medal common vetch extracts
As different from Example 2: concentration of alcohol is 60V%, soak 0.5h, reflux 3 times, each 2h.
The preparation of embodiment 6 silver medal common vetch extracts
As different from Example 2: concentration of alcohol is 80V%, soak 2h, reflux 3 times, each 0.5h.
Below by experimental results show that effective effect of the present invention in experiment in vitro and body
Experimental raw: Lagerstroemia indica L.
l. indicaflower, silver-colored common vetch
l. indical. the flower of f. alba (Nichols.) Rehd.
Lagerstroemia indica L. extract, embodiment 1,2 makes.
Laboratory animal: Kunming mouse, male, body weight 22 ± 2 g, SPF level, is provided production licence number: SCXD Shandong 20080002 by Lukang Medical Co., Ltd., Shandong's Product Quality Verification Centers Animal Lab..
Experiment reagent:
α-glucosidase (α-glucosidase, EC 3.2.1.20) and 4-Nitrobenzol-
α-D-pyranglucoside (4-N-trophenyl-
α-D-glucopyranoside, PNPG, 026 K1516) all purchased from Sigma company of the U.S.; Dimethyl sulfoxine (DMSO, analytical pure, Tianjin De En chemical reagent company limited); Kaliumphosphate buffer (pH 6.8); Glucose assays test kit (ShangHai RongSheng Biology Pharmacy Co., Ltd, 20130503147); Triglyceride (TG) is measured test kit (Zhejiang Dong Ou diagnostic products company limited, Lot:2013060030); T-CHOL (TC) is measured test kit (Zhejiang Dong Ou diagnostic products company limited, Lot:2013050037); Liver/inose unit measures test kit (Bioengineering Research Institute is built up in Nanjing, 20130812); Malonaldehyde (MDA) test kit (Bioengineering Research Institute is built up in Nanjing, 20130812); SOD test kit (surveying total) (Bioengineering Research Institute is built up in Nanjing, 20130813); Acarbose (Acarbose, Lot:130523, Hua Cheng pharmaceutical Co. Ltd of Hangzhou Sino-U.S.); Alloxan (Alloxan monohydrate 98%, Lot:10150059, AlfaAesar); Dehydrated alcohol (AR, Tianjin Fu Yu Fine Chemical Co., Ltd); Concentrated sulphuric acid (AR, Kaifeng Fang Jing chemical reagent company limited); Glacial acetic acid (AR, Kaifeng chemical reagent head factory, 961005).
Experimental apparatus: LRH-150 constant incubator; DELTA 320 type pH meters; Multiskan MK3 microplate reader; Electronic balance; Rotary Evaporators; UV-2000 type ultraviolet-uisible spectrophotometer; CS-H1 type blender; Micropipettor and the rifle of each specification are first-class.
Test example 1 Lagerstroemia indica L. extract is to external
α-Glucosidase inhibitor activity
Experimental technique and result
1, the preparation of sample solution
Lagerstroemia indica L. extract: take the Lagerstroemia indica L. extract of certain mass, add the DMSO of certain volume, being made into initial concentration is 30mg/mL solution.
Acarbose: take the acarbose of certain mass, add the DMSO of certain volume, being made into concentration is 26.67 mg/mL solution;
2,
αthe mensuration of-Glucosidase inhibitor activity
Experimental group arranges as follows:
Blank group: 8 μ L DMSO+152 μ L kaliumphosphate buffers, under 405 nm wavelength, survey OD value;
Blank group: 8 μ L DMSO+112 μ L kaliumphosphate buffer+20 μ L
α-glucosidase+20 μ LPNPG surveys OD value under 405 nm wavelength;
Sample matched group: 8 μ L sample solution+152 μ L kaliumphosphate buffers, under 405 nm wavelength, survey OD value;
Sample sets: 8 μ L sample solution+20 μ L
αμ L kaliumphosphate buffer+20 ,-glucosidase+112 μ LPNPG surveys OD value after reaction finishes under 405 nm wavelength; (detailed process: 112 μ L kaliumphosphate buffers (pH 6.8), adding concentration is 0.2 U/mL
αthe sample solution of-glucosidase 20 μ L, 8 μ L respective concentration, 37 ℃ of constant temperature 15 min, add 20 μ L 2.5 mmol/L PNPG, 37 ℃ of isothermal reaction 15 min.Adding 80 μ L, concentration is the terminator Na of 0.2 mol/L again
2cO
3solution is surveyed OD value under 405 nm wavelength.）
Do blank group under same system, blank group, sample matched group, sample sets simultaneously, calculate suppression ratio by computing formula below, and obtain corresponding IC with Origin 6.0 softwares
50value.
Utilize respectively said determination method to measure Flos lagerstroemiae indicae, silver-colored common vetch is spent each position and positive control acarbose pair
α-glucosidase inhibitor activity.Result is as follows:
Table 1 the different extracted parts
α-glucosidase inhibitor activity
Note :-expression undetermined; The positive contrast of acarbose.
(1) as can be seen from Table 1,, under same primary dcreening operation concentration 1500 μ g/mL, 3 extract parts (PE position, EA position and BU position) of Flos lagerstroemiae indicae and silver-colored common vetch flower are right
α-glucosidase all has higher suppression ratio, and clearance rate is all greater than 90%.For further research inhibition is active, on the basis of primary dcreening operation concentration, dilute successively calculation of half inhibitory concentration IC
50.Result shows, 6 extract parts pair
α-glucosidase all has certain inhibition activity, wherein, and the EA position pair of Flos lagerstroemiae indicae and silver-colored common vetch flower
αthe highest (the IC of inhibition activity of-glucosidase
50=4.45 and 4.09 μ g/ml), be secondly Flos lagerstroemiae indicae and silver-colored common vetch flower BU position (IC
50=17.01 and 14.58 μ g/ml) and PE position (IC
50=103.29 and 112.47 μ g/ml), its activity is all far above positive control acarbose (IC
50=1208.31 μ g/ml).
(2) relation between Flos lagerstroemiae indicae and silver-colored common vetch flower the different extracted parts mass concentration and suppression ratio, as Fig. 1 and Fig. 2.Fig. 1 and Fig. 2 demonstration, in certain mass concentration range, 3 positions pair of Flos lagerstroemiae indicae and silver-colored common vetch flower
αthe inhibition activity of-glucosidase is all dose dependent, after this increases mass concentration again, and suppression ratio is almost constant.
Experimental result shows, Flos lagerstroemiae indicae and silver-colored common vetch are spent 3 extract parts pair
α-glucosidase all has stronger inhibitory action, wherein, suppresses active best with EA position and BU position, illustrates that Flos lagerstroemiae indicae and the larger composition of silver-colored common vetch flower polarity are
α-the active component of glucosidase inhibitor, has the prospect for the preparation of hypoglycemic drug.
Test example 2 Lagerstroemia indica L.s are extracted the protective effect of the diabetic mice to alloxan induction in object
Experimental technique and result
1, experimental technique
Animal grouping and modeling method: the mice of buying back adapts to after 7 d, random packet, 10 of blank groups, another group is diabetes modeling group.Diabetes modeling group be can't help water 12-16 in fasting and is caused diabetes model by the disposable tail vein injection alloxan of 80 mg/kg after h hour.Normal nursing after 72 h, water 12 h are can't help in fasting, and eye socket is got blood, survey fasting blood glucose level, according to blood glucose homeostatic principle, the mice by blood sugar level more than 11.1 mmol/L is divided into 14 groups, 10 every group: the high, medium and low dosage group in Flos lagerstroemiae indicae EA position, the high, medium and low dosage group in Flos lagerstroemiae indicae BU position, the high, medium and low dosage group in silver common vetch flower EA position, the high, medium and low dosage group in silver-colored common vetch flower BU position, acarbose positive controls, diabetic model group, Normal group is as blank group.Except model group and blank group, other groups corresponding medicine of gavage respectively, 1 time/d, successive administration 7 d, after last administration, 2 h eye sockets are got blood and are surveyed level of postprandial blood sugar, and in fasting on that night 12 h, 8d wins eyeball and gets blood, separation of serum, is positioned over refrigerator 0-3 ℃ preservation, for measuring fasting glucose after administration, TG, TCH, MDA and SOD.Put to death animal, get liver and weigh, for measuring liver glycogen content.
2, experimental result is as follows
The impact of table 2 the different extracted parts on blood glucose in diabetic mice (
`
x±
s , n=10)
Note: the positive contrast of acarbose;
With the comparison of blank group:
△ △ △ p< 0.001,
△ △ p< 0.01,
△ p< 0.05;
With model group comparison:
* * p< 0.001,
* p< 0.01,
* p< 0.05;
With positive controls comparison:
### p< 0.001,
## p< 0.01,
# p< 0.05;
The impact of table 3 the different extracted parts on diabetic mice liver glycogen (
`
x±
s , n=10)
Note: the positive contrast of acarbose;
With the comparison of blank group:
△ △ △ p< 0.001,
△ △ p< 0.01,
△ p< 0.05;
With model group comparison:
* * p< 0.001,
* p< 0.01,
* p< 0.05;
With positive controls comparison:
### p< 0.001,
## p< 0.01,
# p< 0.05.
The impact of table 4 the different extracted parts on TCH and TG in diabetic mice serum (
`
x±
s , n=10)
Note: the positive contrast of acarbose;
With the comparison of blank group:
△ △ △ p< 0.001,
△ △ p< 0.01,
△ p< 0.05;
With model group comparison:
* * p< 0.001,
* p< 0.01,
* p< 0.05;
With positive controls comparison:
### p< 0.001,
## p< 0.01,
# p< 0.05.
The impact of table 5 the different extracted parts on diabetic mice serum MDA and SOD (
`
x±
s , n=10)
Note: the positive contrast of acarbose;
With the comparison of blank group:
△ △ △ p< 0.001,
△ △ p< 0.01,
△ p< 0.05;
With model group comparison:
* * p< 0.001,
* p< 0.01,
* p< 0.05;
With positive controls comparison:
### p< 0.001,
## p< 0.01,
# p< 0.05
(1) as can be seen from Table 2, before administration, compared with blank group, diabetic groups (Flos lagerstroemiae indicae PE position, Flos lagerstroemiae indicae EA position, Flos lagerstroemiae indicae BU position, silver-colored common vetch flower PE position, silver-colored common vetch flower EA position, silver-colored common vetch flower BU position positive controls and model group) fasting glucose be all utmost point significance raise (
p<0.001), alloxan induction diabetic mice modeling success is described, and there was no significant difference between each administration group.
After drug treatment 7d, compared with blank group, model group post-prandial glycemia be utmost point significance raise (
p<0.001), the visible diabetes post-prandial glycemia that can raise; Compared with model group, the equal energy of dosage group and BU position high dose group significance ground reduction post-prandial glycemia in Flos lagerstroemiae indicae EA position (
p<0.05), other each groups reduce postprandial plasma glucose levels lower than model group, but do not have significance (
p>0.05).
Compared with model group, Flos lagerstroemiae indicae EA position and BU position and silver-colored common vetch flower EA position and the each dosage group in BU position and positive controls all can reduce fasting glucose by significance; Compared with positive controls, Flos lagerstroemiae indicae BU position low dose group, silver-colored common vetch flower EA position low dose group and the equal energy of silver-colored common vetch flower BU position high dose group significance ground rising fasting glucose (
p<0.05,
p<0.001,
p<0.001), other respectively organize there was no significant difference.
From administration, post-prandial glycemia and fasting glucose are comprehensively analyzed, and all there is certain hypoglycemic activity at EA position and the BU position of Flos lagerstroemiae indicae and silver-colored common vetch flower.
(2) as can be seen from Table 3, compared with blank group, the reduction of model group Mouse Liver glycogen content utmost point significance (
p<0.05); After drug treatment 7 d, compared with model group, Flos lagerstroemiae indicae EA position low dose group, silver-colored common vetch flower high, the middle dosage group in EA position and the equal energy of positive controls significance ground rising liver glycogen content (
p<0.05), although the content of other the each group liver glycogen that can raise does not have significant difference.Visible, the each dosage group in EA position and BU position of Flos lagerstroemiae indicae and silver-colored common vetch flower can, by promoting the approach such as the synthetic of hepatic glycogen, the decomposition of reduction liver glycogen, reduce the blood sugar level of diabetes.
(3) as can be seen from Table 4, compared with blank group, the rising of model group mice TC content significance (
p<0.05), TG content raise, but do not have significant difference (
p>0.05); After drug treatment 7 d, compared with model group, EA position and the BU position of Flos lagerstroemiae indicae and silver-colored common vetch flower all reduce TC content in various degree to dosage group significance; The content of the each dosage group of low dose group and BU position senior middle school and the equal energy of positive control significance ground reduction TG in the each dosage of Flos lagerstroemiae indicae EA position low dose group and BU position senior middle school, silver-colored common vetch flower EA position (
p<0.05), though other each dosage groups can reduce the content of TG, do not have significant difference (
p>0.05).Visible, diabetic complication-hyperlipidemia can, by the content of TC and TG in reduction serum, be improved in the EA position of Flos lagerstroemiae indicae and silver-colored common vetch flower and BU position, corrects the lipid metabolic disorder of diabetic mice.
(4) as can be seen from Table 5, compared with blank group, MDA content significance rising in model group mice serum (
p<0.01), SOD level utmost point significance reduction (
p<0.001), visible, diabetes can cause that vivo oxidation stress.After drug treatment 7 d, compared with model group, the each dosage group in Flos lagerstroemiae indicae EA position and BU position all can significantly reduce the content of MDA in various degree, silver-colored common vetch flower EA position low dose group and BU position high dose group can significantly reduce MDA content (
p<0.05,
p<0.01), although other each group can reduce the content of MDA, do not have significant difference (
p>0.05); All increased SOD levels to some extent of the each dosage group in EA position and BU position of Flos lagerstroemiae indicae and silver-colored common vetch flower.Visible, the EA position of Flos lagerstroemiae indicae and silver-colored common vetch flower and BU position all can reduce the content of MDA in serum to some extent, increased SOD level, and the anti-oxidation function of enhancing diabetic mice body, protection body is avoided the further injury of free radical.
Claims (9)
1. a Lagerstroemia indica L. extract, is characterized in that, is petroleum ether part and/or ethyl acetate extract and/or the n-butanol portion of the flower of Lythraceae plant of Lagerstroemia.
2. Lagerstroemia indica L. extract as claimed in claim 1, it is characterized in that, petroleum ether part, ethyl acetate extract and n-butanol portion obtain by the following method: take the flower of Lythraceae plant of Lagerstroemia as raw material, first obtain total ethanol extractum with ethanol extraction, again total ethanol extractum is scattered in water, extract with petroleum ether, ethyl acetate, n-butyl alcohol, the petroleum ether extract, acetic acid ethyl ester extract and the n-butyl alcohol extract that obtain are respectively petroleum ether part, ethyl acetate extract and n-butanol portion.
3. Lagerstroemia indica L. extract as claimed in claim 1 or 2, is characterized in that, described Lythraceae plant of Lagerstroemia is: Lagerstroemia indica L.
lagerstroemia indicaor silver-colored common vetch L.
lagerstroemia indical. f. alba (Nichols.) Rehd.
4. Lagerstroemia indica L. extract as claimed in claim 2, it is characterized in that, described total ethanol extractum obtains by the following method: Lythraceae plant of Lagerstroemia spend first drying, pulverizing, then use soak with ethanol, heating and refluxing extraction 2-3 time, 0.5-2h at every turn, merge extractive liquid,, filtration, the concentrated total ethanol extractum that to obtain.
5. the extracting method of Lagerstroemia indica L. extract described in claim 1, it is characterized in that: Lythraceae plant of Lagerstroemia spend first drying, pulverizing, then use soak with ethanol, heating and refluxing extraction 2-3 time, each 0.5-2h, merge extractive liquid,, filtration, concentratedly to obtain total ethanol extractum, then total ethanol extractum is scattered in water, extract with petroleum ether, ethyl acetate, n-butyl alcohol, the petroleum ether extract, acetic acid ethyl ester extract and the n-butyl alcohol extract that obtain are Lagerstroemia indica L. extract.
6. the extracting method of Lagerstroemia indica L. extract as claimed in claim 5, is characterized in that, concentration of alcohol is 65～80V%.
7. Lagerstroemia indica L. extract claimed in claim 1 is in preparation
αapplication in-glucosidase inhibitor.
8. Lagerstroemia indica L. extract claimed in claim 1 is in the application of preparing aspect hypoglycemic drug.
9. the application of Lagerstroemia indica L. extract claimed in claim 1 aspect preparation treatment diabetes medicament.
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|---|---|---|---|---|
| CN104069348A (en) * | 2014-06-30 | 2014-10-01 | 天津中医药大学 | Sealwort extract as well as preparation method and use thereof |
| CN104435071A (en) * | 2014-11-06 | 2015-03-25 | 河南大学 | Active part of mallotus oblongifolius as well as extraction method and application thereof |
| CN105497413A (en) * | 2015-12-21 | 2016-04-20 | 新乡医学院 | Chinese yam overground part extract and preparing method and application thereof |
| CN109169258A (en) * | 2018-09-27 | 2019-01-11 | 湛江恒达园林研发有限公司 | A kind of no fruiting crape myrtle breeding method |
| CN110477253A (en) * | 2019-08-20 | 2019-11-22 | 苏州科技大学 | Crape myrtle fruit Substance extraction process and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040039132A (en) * | 2002-11-04 | 2004-05-10 | 주식회사 태평양 | Cosmetic composition containing lagerstroemia indica flower extract for repairing wrinkle and roughness of the skin |
| CN101416971A (en) * | 2008-12-11 | 2009-04-29 | 浙江大学 | Use of betulinic acid in preparing glycosidase inhibitor medicine |
| KR20110001538A (en) * | 2009-06-30 | 2011-01-06 | 한국콜마 주식회사 | Flowers extract having anti-oxidation and whitening effects and extraction method thereof and cosmetics comprising flowers extract |
| KR20110050938A (en) * | 2009-11-09 | 2011-05-17 | 대전대학교 산학협력단 | Extracts of lagerstroemia indica for allergy treating and pharmaceutical compositions for allergy comprising the extracts |
-
2014
- 2014-01-16 CN CN201410019586.6A patent/CN103860611A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040039132A (en) * | 2002-11-04 | 2004-05-10 | 주식회사 태평양 | Cosmetic composition containing lagerstroemia indica flower extract for repairing wrinkle and roughness of the skin |
| CN101416971A (en) * | 2008-12-11 | 2009-04-29 | 浙江大学 | Use of betulinic acid in preparing glycosidase inhibitor medicine |
| KR20110001538A (en) * | 2009-06-30 | 2011-01-06 | 한국콜마 주식회사 | Flowers extract having anti-oxidation and whitening effects and extraction method thereof and cosmetics comprising flowers extract |
| KR20110050938A (en) * | 2009-11-09 | 2011-05-17 | 대전대학교 산학협력단 | Extracts of lagerstroemia indica for allergy treating and pharmaceutical compositions for allergy comprising the extracts |
Non-Patent Citations (4)
| Title |
|---|
| NABIEL A. M. SALEH: "Anthocyanins of Lagerstroemia indica flowers", 《PHYTOCHEMISTRY》, vol. 12, 31 December 1973 (1973-12-31), pages 2304 * |
| S. F. HUSSAIN ET AL: "3,4,3’-TRI-0-METHYLELLAGIC ACID FROM LAGERSTROEMIA INDICA", 《PHYTOCHEMISTRY》, vol. 11, 31 December 1972 (1972-12-31), pages 2890 - 2891 * |
| 晏丽等: "紫薇花绿原酸超声波辅助提取研究", 《湖北农业科学》, vol. 50, no. 1, 31 January 2011 (2011-01-31), pages 146 - 147 * |
| 王燕等: "紫薇属植物的化学成分和药理作用研究进展", 《药学实践杂志》, vol. 28, no. 2, 25 March 2010 (2010-03-25), pages 88 - 93 * |
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| CN109169258A (en) * | 2018-09-27 | 2019-01-11 | 湛江恒达园林研发有限公司 | A kind of no fruiting crape myrtle breeding method |
| CN109169258B (en) * | 2018-09-27 | 2021-06-01 | 湛江恒达园林研发有限公司 | Crape myrtle cultivation method without bearing fruits |
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