CN103768151B - Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract is preparing the application in antiaging agent or health product - Google Patents
Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract is preparing the application in antiaging agent or health product Download PDFInfo
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- CN103768151B CN103768151B CN201410043488.6A CN201410043488A CN103768151B CN 103768151 B CN103768151 B CN 103768151B CN 201410043488 A CN201410043488 A CN 201410043488A CN 103768151 B CN103768151 B CN 103768151B
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- Prior art keywords
- flos osmanthi
- osmanthi fragrantis
- phenethyl alcohol
- alcohol glycoside
- solution
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Abstract
The invention discloses a kind of Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract and prepare the application in antiaging agent or health product; In Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, phenylethanoid glycosides content is 30 ~ 90%(% by weight), described phenylethanoid glycosides comprises verbascoside, different verbascoside.In Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, verbascoside content is 25 ~ 80%(% by weight), in phenylethanoid glycosides, verbascoside is 48:1 ~ 300:1 with the ratio of the weight of different verbascoside.
Description
Technical field
The present invention relates to agricultural byproducts intensive processing and field of comprehensive utilization.Relate more specifically to a kind of phenethyl alcohol glycoside extract of extracting from Flos Osmanthi Fragrantis and method for making thereof and purposes-for the preparation of antiaging agent or health product.
Background technology
Flos Osmanthi Fragrantis, have another name called wooden slippers, rock osmanthus, one of large flowers of Chinese tradition ten, extensively plant in Basin of Huaihe River and areas to the south, current China formed Zhejiang Hangzhou, Anhui six is pacified, xianning,hubei, Fauna of Taoyuan, Nw Hunan, Law Firm Suzhou Jiangsu, Guilin, several large Flos Osmanthi Fragrantis commodity production base such as Wuhan, Hubei and Chengdu, Sichuan.Osmanthus cultivars is various, and nearly 152 kinds, can be divided into four strains, i.e. Osmanthus fragans, Osmanthus tragranscv.Aurantiacu, silver-colored osmanthus, Osmanthus fragrans var.thunbergii..Flos Osmanthi Fragrantis, except viewing and admiring and beautifying, also has higher nutrition and medical value.The traditional Chinese medical science thinks that Flos Osmanthi Fragrantis is the length of hundred medicines, with the wine of Flos Osmanthi Fragrantis brew appetizing refreshment, stomach invigorating tonify deficiency, can reach the effect of " the longevity Your Highness of drink ", osmanthus flower tea has looks improving and the skin nourishing, throat of releiving, improve the effects such as excessive phlegm cough.Flos Osmanthi Fragrantis is also made into the cuisines such as Oryza glutinosa Flos Osmanthi Fragrantis Rhizoma Nelumbinis, sweet-scented cake, Flos Osmanthi Fragrantis almond curd in syrup, by the general common people is liked.
But, the research of people to the many functional components in Flos Osmanthi Fragrantis is up to now also considerably less, mainly concentrate on Flos Osmanthi Fragrantis and compose fragrant composition and a few medicinal ingredient, as linalool, γ-decalactone, alpha, beta-lonone, 4-hydrogen-alpha, beta-lonone, Palmic acid, alpha-linolenic acid.Also have part the flavones in Flos Osmanthi Fragrantis and Polyphenols component to be carried out to the research of separation and Extraction purification, but be only limitted to the research of crude extract, have no the analysis of monomeric compound.
The present inventor's patent of invention 201310169455.1 in earlier stage discloses a kind of Flos Osmanthi Fragrantis polyphenol extract, the weight content of its phenolic acid is 0.5% ~ 2%, the weight content of phenethyl alcohol glycoside is 50% ~ 80%, the weight content of its Content of Chlorogenic Acid is 40% ~ 70%, chlorogenic acid, caffeic acid and P-coumaric acid mass ratio are 25 ~ 50:5 ~ 15:6 ~ 10, and in phenethyl alcohol glycoside, the weight content of verbascoside is 80% ~ 95%; This patent also discloses a kind of preparation method-high-pressure microwave extraction method simultaneously.But along with going deep into of research, we find in Flos Osmanthi Fragrantis also containing other the phenethyl alcohol glycoside compounds except verbascoside.In addition, traditional Flos Osmanthi Fragrantis polyphenol does not pay close attention to the stability of Phenylethanoid glycosides in extracting, and therefore affects to some extent extraction yield and extract purity, therefore, needs the oxidation Sum decomposition taking measures to prevent phenethyl alcohol glycoside in leaching process.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract containing different verbascoside and is preparing the application in antiaging agent or health product.
In order to solve the problems of the technologies described above, the invention provides a kind of Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract and preparing the application in antiaging agent or health product.
As Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of the present invention in the improvement preparing the application in antiaging agent or health product:
In Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, phenylethanoid glycosides content is 30 ~ 90%(% by weight), described phenylethanoid glycosides comprises verbascoside, different verbascoside.
The further improvement of the application in antiaging agent or health product is being prepared: in described Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, verbascoside content is 25 ~ 80%(% by weight as Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of the present invention), in phenylethanoid glycosides, verbascoside is 48:1 ~ 300:1 with the ratio of the weight of different verbascoside.
Remarks illustrate: verbascoside accounts for about 90% of phenylethanoid glycosides content.
The further improvement of the application in antiaging agent or health product is being prepared: in described Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, phenylethanoid glycosides content is 40 ~ 80% as Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of the present invention;
In described Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, verbascoside content is 35 ~ 70%, and in phenylethanoid glycosides, verbascoside and the ratio of the weight of different verbascoside are 48:1 ~ 150:1(is such as 48:1,77:1,80 ~ 150:1).
The further improvement of the application in antiaging agent or health product is being prepared: the preparation method of Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract comprises the following steps as Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of the present invention:
(a), by broken for Flos Osmanthi Fragrantis dry flower (moisture≤10%) (about 20 ~ 40 object powder), be added in ethanol water, then add the aqueous solution of antioxidant or antioxidant, then add weak acid until pH is 4 ~ 6, obtain extraction solution;
In described ethanol water, the volumetric concentration of ethanol is 30 ~ 99.9%(v/v); Above-mentioned concentration is preferably 50 ~ 90%;
The ethanol water of described every 1KG Flos Osmanthi Fragrantis dry flower adapted 10 ~ 15L;
The weight ratio of described antioxidant and Flos Osmanthi Fragrantis dry flower is: 0.05 ~ 10%; Above-mentioned weight ratio is preferably 0.2%;
(b), extraction solution is carried out lixiviate 1.5 ~ 3.0 hours at 50 ~ 80 DEG C, after being cooled to room temperature, filter or centrifugal, obtain extracting solution;
(c), by extracting solution directly through concentrating under reduced pressure reclaim ethanol, obtain concentrated solution; Again by concentrated solution dilute with water, until the pH of gained diluent is 4-6;
(d), diluent is adsorbed through adsorbent resin, be the ethanol water eluting of 20 ~ 50% by volumetric concentration;
E, after (), eluent concentrating under reduced pressure, dry (being such as spraying dry, low pressure oven dry or microwave drying), obtains Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract.
The further improvement of the application in antiaging agent or health product is being prepared as Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of the present invention:
Described antioxidant is ascorbic acid, ascorbyl palmitate, arabo-ascorbic acid, sodium erythorbate, vitamin E, carotenoid, Folium Bambusae antioxidant or tea polyphenols;
Described weak acid is formic acid, acetic acid, hydroxyacetic acid, lactic acid, tartaric acid, malic acid, fumaric acid, ascorbic acid, cinnamic acid, benzoic acid, ferulic acid, caffeic acid, gallic acid or phosphoric acid.
The further improvement of the application in antiaging agent or health product is being prepared: described Flos Osmanthi Fragrantis is Osmanthus fragrans var.thunbergii., silver-colored osmanthus, Osmanthus fragans or Osmanthus tragranscv.Aurantiacu as Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of the present invention.
The further improvement of the application in antiaging agent or health product is being prepared: preparation method comprises the following steps as Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of the present invention:
(a), 1kg Osmanthus tragranscv.Aurantiacu dry flower fragmentation (moisture≤10%) is crushed to and can crosses 20 objects sieves, being added to 10L volumetric concentration is in the ethanol water of 90%, add AOB solution 2L again, utilizing 1mol/L acetic acid aqueous solution to adjust pH is 5, obtains extraction solution;
Containing 1g AOB in described often liter of AOB solution;
(b), extraction solution is carried out lixiviate 2 hours at 50 ~ 80 DEG C, after being cooled to room temperature, filter or centrifugal, obtain extracting solution;
(c), by extracting solution directly through concentrating under reduced pressure reclaim ethanol, obtain concentrated solution; Again by concentrated solution dilute with water, until the pH of gained diluent is 4;
(d), diluent is adsorbed through adsorbent resin, be the ethanol water eluting of 30% by volumetric concentration;
E, after (), eluent concentrating under reduced pressure, spray-dried, low pressure is dried or microwave drying, can obtain Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract.
Preparing the application in antiaging agent or health product as Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of the present invention, preparation method comprises the following steps:
(a), 1kg Osmanthus fragrans var.thunbergii. dry flower fragmentation (moisture content≤10%) is crushed to and can crosses 20 objects sieves, being added to 15L volumetric concentration is in 80% ethanol water, add arabo-ascorbic acid sodium water solution 2L again, utilizing the adjustment of 1mol/L aqueous formic acid to extract pH value of solution is 5, obtains extraction solution;
Containing 1g sodium erythorbate in often liter of arabo-ascorbic acid sodium water solution;
(b), extraction solution is carried out lixiviate 2 hours at 50 ~ 80 DEG C, after being cooled to room temperature, filter or centrifugal, obtain extracting solution;
(c), extracting solution is reclaimed ethanol through concentrating under reduced pressure, obtain concentrated solution; Dilute with water again, until the pH of gained diluent is 5;
(d), diluent is adsorbed through adsorbent resin, be the ethanol water eluting of 30% by volumetric concentration;
E, after (), eluent concentrating under reduced pressure, spray-dried, low pressure is dried or microwave drying, obtains Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract.
In the present invention, room temperature generally refers to 10 ~ 25 DEG C.
The present inventor, through furtheing investigate for many years, has carried out extraction and isolation to the various compositions of Flos Osmanthi Fragrantis, obtains Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, shows the research of this extract, and this extract is mainly containing the composition such as verbascoside, different verbascoside.Complete the present invention on this basis.
As used herein, Flos Osmanthi Fragrantis (Osmanthusfragrans) refers to the flower of osmanthus fragrans.
The raw material that the present invention adopts can carry out pretreatment, also can without pretreatment.A kind of preferred feedstock is the Flos Osmanthi Fragrantis of powder type.Such as, collect fresh Flos Osmanthi Fragrantis, pulping squeezing or collection Flos Osmanthi Fragrantis dry flower, be crushed to about 20 ~ 40 object powder.
Osmanthus cultivars used in the present invention is not particularly limited, and can be the Flos Osmanthi Fragrantis of different cultivars, preferred Osmanthus fragrans var.thunbergii., silver-colored osmanthus, Osmanthus fragans, Osmanthus tragranscv.Aurantiacu.
Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of the present invention is a kind of based on the mixture of verbascoside, different verbascoside.With spectrophotometry, be standard substance with verbascoside, the content recording phenylethanoid glycosides in extract is 30 ~ 90% (being preferably 40 ~ 80%); Use HPLC technical Analysis, in extract, verbascoside content is 25 ~ 80% (being preferably 35 ~ 70%), and verbascoside and the ratio of different verbascoside content are 48:1 ~ 300:1 (being preferably 48:1 ~ 150:1).
For pharmaceutical composition or Halth-care composition, they can be solid-state (as granule, tablet, lyophilized powder, suppository, capsule, sublingual lozenge) or liquid (as oral liquid) or other suitable shapes.The content of Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of the present invention is generally 1% ~ 99% of composition weight, is preferably 2% ~ 96%, is more preferably 5% ~ 90%, and best 15% ~ 80%.
Halth-care composition can be single dose or multi-dose formats.By applied agents gauge, usually containing 10 ~ 2000mg/ agent, preferably about 20 ~ 1000mg/ agent, more preferably 50 ~ 500mg/ agent.
Pharmaceutical composition of the present invention or Halth-care composition can carry out administration by conventional route, and optimal way is oral.Pharmaceutical preparation should match with administering mode.Amount of application of the present invention, calculates by active substance, is generally Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract 0.1 ~ 500mg/kg body weight every day, preferably about 0.5 ~ 50mg/kg body weight.In addition, preparation of the present invention also can use together with other auxiliary agents, and such as, other auxiliary agents comprise various existing anti-cancer active matter or anti-oxidation active substance.
Major advantage of the present invention is:
(a) provide a kind of originate wide, form distinct, best in quality, quality controllable, of many uses and there is the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of defying age purposes.
B () achieves the high efficiency extraction of phenethanol glucosides in Flos Osmanthi Fragrantis by the technology of the present invention, and technological operation is easy, and the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract quality obtained is good, stay in grade.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Fig. 1 is the liquid chromatogram of Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract.
Fig. 2 is the liquid chromatogram of verbascoside and different verbascoside.
Fig. 3 is the chemical structural drawing of verbascoside and different verbascoside.
Detailed description of the invention
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually conveniently condition, or according to the condition that manufacturer advises.
Universal method
1) phenylethanoid glycosides assay:
50.0mg Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract is dissolved in methanol, be settled to 100ml, get 3ml add in quartz colorimetric utensil with ultraviolet spectrophotometer 338nm place detection its light absorption value, replication 3 times, take verbascoside as standard substance, calculate phenylethanoid glycosides content according to standard curve.
2) verbascoside and different verbascoside assay-high performance liquid chromatography (HPLC) in Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract:
Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract adds 2%(volume %) acetic acid solution dissolving, make 500 μ g/mL solution, cross 0.45 μm of film, sample introduction.The efficient liquid phase of high performance liquid chromatograph device: Shimadzu LC-2010A, SPD-20A UV-detector, BDSC18 post (4.6mm × 250mm, 5 μm of i.d.), mobile phase: acetonitrile: 2% acetum (volume ratio of 20:80), determined wavelength: 330nm, flow velocity: 0.8mL/min, column temperature: 35 DEG C, sampling volume 10 μ L.With verbascoside and different verbascoside for standard substance, qualitative according to retention time, according to peak area quantified by external standard method.
Embodiment 1:
By 1kg Osmanthus fragrans var.thunbergii. dry flower fragmentation (moisture content≤10%, be crushed to and can cross 20 object sieves), add the 80%(volume % of 15L) in ethanol water, add the arabo-ascorbic acid sodium water solution of 0.1% again (namely, containing 1g sodium erythorbate in described often liter of arabo-ascorbic acid sodium water solution) 2L, utilize the adjustment of 1mol/L aqueous formic acid to extract pH value of solution about 5; At temperature 50 C ~ 80 DEG C, carry out lixiviate 2 hours, after being cooled to room temperature, filtering and obtain extracting solution; By extracting solution directly through concentrating under reduced pressure (in 0.010 ~ 0.100MPa, temperature is 50 ~ 60 DEG C), reclaim ethanol, obtain concentrated solution (about 4L), then dilute with water, until diluent pH is about 5; By diluent through adsorbent resin absorption (adsorbent resin is HPD300, and consumption is 1L), with 30%(volume %) ethanol water 2L eluting; After eluent concentrating under reduced pressure (in 0.010 ~ 0.100MPaMPa, temperature is 75 ~ 90 DEG C), spray-dried (inlet temperature is 180 ~ 200 DEG C, and leaving air temp is 85 ~ 95 DEG C), can obtain Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract powder 184g.
Component analysis is carried out to extract, result is: in Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, phenylethanoid glycosides content is 75.3%, Fig. 1-3 shows, Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract contains verbascoside (69.4%) and different verbascoside (0.9%), verbascoside: different verbascoside=77:1(weight ratio).
Comparative example 1-1, made into " straight alcohol of 12L " by " the 80%(volume % of 15L) ethanol water " in embodiment 1, all the other are with embodiment 1.
Finally can obtain Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract powder 171g.In the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of gained, phenylethanoid glycosides content is 70.5%, and Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract contains verbascoside (65.6%) and different verbascoside (0.9%).
Comparative example 1-2, " the 80%(volume % of 15L) ethanol water " in embodiment 1 is made into " the 60%(volume % of 20L) ethanol water ", all the other are with embodiment 1.
Finally can obtain Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract powder 187g.In the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of gained, phenethyl alcohol glycoside content is 71.2%, and Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract contains verbascoside (67.3%) and different verbascoside (0.8%).
Comparative example 2-1, " 0.1% arabo-ascorbic acid sodium water solution 2L " to be made into: 0.04% arabo-ascorbic acid sodium water solution (that is, in described often liter of arabo-ascorbic acid sodium water solution containing 0.4g sodium erythorbate) 2L; All the other are with embodiment 1.
Finally can obtain Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract powder 123.8g.In the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of gained, phenylethanoid glycosides content is 25.2%, and Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract contains verbascoside (23.4%) and different verbascoside (0.2%).
Comparative example 2-2, " 0.1%(quality %) arabo-ascorbic acid sodium water solution 2L " to be made into: 1.0% arabo-ascorbic acid sodium water solution (that is, in described often liter of arabo-ascorbic acid sodium water solution containing 10g sodium erythorbate) 2L; All the other are with embodiment 1.
Finally can obtain Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract powder 172.7g.In the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of gained, phenylethanoid glycosides content is 75.3%, and Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract contains verbascoside (69.4%) and different verbascoside (0.9%).
Comparative example 3-1, makes adjustment pH to 5 into adjustment 3.5, and all the other are with embodiment 1.
Finally can obtain Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract powder 160.6g.In the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of gained, phenylethanoid glycosides content is 64.2%, and Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract contains verbascoside (60.5%) and different verbascoside (0.7%).
Comparative example 3-2, makes adjustment pH to 5 into adjustment 6.5, and all the other are with embodiment 1.
Finally can obtain Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract powder 151.9g.In the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of gained, phenylethanoid glycosides content is 61.1%, and Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract contains verbascoside (56.8%) and different verbascoside (0.7%).
Comparative example 4-1, " carrying out lixiviate 2 hours at 50 DEG C ~ 80 DEG C " is made into " carrying out lixiviate 4 hours at 30 DEG C ~ 49 DEG C ", all the other are with embodiment 1.
Finally can obtain Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract powder 142.8g.In the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of gained, phenylethanoid glycosides content is 74%, and Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract contains verbascoside (68.5%) and different verbascoside (0.85%).
Comparative example 4-2, " carrying out lixiviate 2 hours at 50 DEG C ~ 80 DEG C " is made into " carrying out lixiviate 2 hours at 90 DEG C ~ 100 DEG C ", all the other are with embodiment 1.
Finally can obtain Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract powder 194.6g.In the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of gained, phenylethanoid glycosides content is 70.5%, and Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract contains verbascoside (66.8%) and different verbascoside (0.9%).
Embodiment 2:
By 1kg Osmanthus fragans dry flower fragmentation (moisture content≤10%, be crushed to and can cross 20 object sieves), add the 90%(volume % of 10L) in ethanol water, add 0.2% aqueous ascorbic acid again (namely, containing 2g ascorbic acid in described often liter of aqueous ascorbic acid) 2L, utilize the adjustment of 0.5mol/L acetic acid aqueous solution to extract pH value of solution about 4; At temperature 50 C ~ 80 DEG C, carry out lixiviate 2 hours, after being cooled to room temperature, filtering and obtain extracting solution; By extracting solution directly through concentrating under reduced pressure (in 0.010 ~ 0.100MPa, temperature is 50 ~ 60 DEG C), reclaim ethanol, obtain concentrated solution (about 1.5L), then dilute with water, until diluent pH is about 4; By diluent through adsorbent resin absorption (described adsorbent resin is HPD300, and consumption is 1L), with 30%(volume %) ethanol water 2L eluting; After eluent concentrating under reduced pressure (in 0.010 ~ 0.100MPaMPa, temperature is 75 ~ 90 DEG C), through microwave drying (technological parameter is: microwave power 500W, microwave irradiation time 60min), Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract powder 234g can be obtained.
Component analysis is carried out to extract, in result Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, phenylethanoid glycosides content is 47.3%, Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract contains verbascoside (40.5%) and different verbascoside (0.2%), verbascoside: different verbascoside=203(weight ratio).
Embodiment 3:
By 1kg Osmanthus tragranscv.Aurantiacu dry flower fragmentation (moisture content≤10%, be crushed to and can cross 20 object sieves), be added to the 90%(volume % of 10L) in alcoholic solution, add 0.1% AOB aqueous solution again (namely, containing 1g AOB in described often liter of AOB aqueous solution) 2L, utilize the adjustment of 1mol/L acetic acid aqueous solution to extract pH value of solution about 5; Solution must be extracted; Extraction solution is carried out lixiviate 2 hours at temperature 50 C ~ 80 DEG C, after being cooled to room temperature, filtering and obtain extracting solution; By extracting solution directly through concentrating under reduced pressure (in 0.010 ~ 0.100MPa, temperature is 50 ~ 60 DEG C), reclaim ethanol, obtain concentrated solution (about 2.5L), then dilute with water, until diluent pH is about 4; By diluent through adsorbent resin absorption (described adsorbent resin is HPD300, and consumption is 1L), with 30%(volume %) ethanol water 2L eluting; After eluent concentrating under reduced pressure (in 0.010 ~ 0.100MPaMPa, temperature is 75 ~ 90 DEG C), through microwave drying (technological parameter is: microwave power 500W, microwave irradiation time 60min), Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract powder 258g can be obtained.
Component analysis is carried out to extract, in result Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, phenylethanoid glycosides content is 74.5%, Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract contains verbascoside (68.9%) and different verbascoside (1.4%), verbascoside: different verbascoside=48(weight ratio).
Embodiment 4:
By 1kg silver osmanthus dry flower fragmentation (moisture content≤10%, be crushed to and can cross 20 object sieves), add the 50%(volume % of 15L) in alcoholic solution, add 0.1% aqueous ascorbic acid again (namely, containing 1g ascorbic acid in described often liter of aqueous ascorbic acid) 2L, utilize the adjustment of 1mol/L acetic acid aqueous solution to extract pH value of solution about 4, obtain extraction solution; Extraction solution is carried out lixiviate 2 hours at temperature 50 C ~ 80 DEG C, after being cooled to room temperature, filtering, obtain extracting solution; By extracting solution directly through concentrating under reduced pressure (in 0.010 ~ 0.100MPa, temperature is 50 ~ 60 DEG C), reclaim ethanol, obtain concentrated solution (about 10L), then dilute with water, until diluent pH is about 4; By diluent through adsorbent resin absorption (described adsorbent resin is HPD300, and consumption is 1L), with 30%(volume %) ethanol water 2L eluting; After eluent concentrating under reduced pressure (in 0.010 ~ 0.100MPaMPa, temperature is 75 ~ 90 DEG C), through microwave drying (technological parameter is: microwave power 500W, microwave irradiation time 60min), Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract powder 107g can be obtained.
Component analysis is carried out to extract, in result Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, phenylethanoid glycosides content is 86.1%, Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract contains verbascoside (79.3%) and different verbascoside (0.3%), verbascoside: different verbascoside=264(weight ratio).
Embodiment 5, scavenging free radicals
(1) DPPH method
The DPPH accurately taking 20mg before experiment is dissolved in proper amount of methanol, is settled to 500ml.With 25-800 μM of Trolox for reference substance, get sample or the reference substance solution 100ul of dilution certain multiple, add 3.9mlDPPH, 37 DEG C of lucifuge reaction 60min, survey absorbance in 515nm wavelength place.With Trolox concentration for abscissa, light absorption value is that vertical coordinate makes standard curve, and according to standard curve calculation sample oxidation resistance, result represents (mmolTrolox/gDW) with Trolox equivalent in every gram of extract.
(2) ABTS method
Configure 7.4mMABTS and 2.6mM potassium peroxydisulfate respectively, equal-volume mixing before use, 37 DEG C of lucifuge reaction 12h, use after diluting 27 times.With 25-800 μM of Trolox for reference substance, get the sample after dilution certain multiple or reference substance solution 100ul, add 3.0mlABTS reactant liquor, after 37 DEG C of lucifuge reaction 60min, survey absorbance in 734nm place.With Trolox concentration for abscissa, light absorption value is that vertical coordinate makes standard curve, and according to standard curve calculation sample oxidation resistance, result represents (mmolTrolox/gDW) with Trolox equivalent in every gram of extract.
(3) FRAP method
Take 40mMHCl as solvent configuration 10mMTPTZ solution, 20mMFeCl
3aqueous solution, 300mM, pH=3.6 sodium acetate buffer, three is with 1:1:10(v/v/v) be mixed to get FRAP.With FeSO
4for reference substance, get the sample after dilution certain multiple or reference substance solution 100ul, add 3.0mlFRAP reactant liquor, after 37 DEG C of lucifuge reaction 60min, survey absorbance in 595nm place.With FeSO4 concentration for abscissa, light absorption value is that vertical coordinate makes standard curve, and according to standard curve calculation sample oxidation resistance, result represents (mmolFeSO with FeSO4 equivalent in every gram of extract
4/ gDW).
(4) ORAC method
Configuration pH7.4, concentration is 75mM phosphate buffer, with it for solvent preparation fluorescein and AAPH solution.With 0-80 μM of Trolox for reference substance, respectively to adding 20ul sample (after dilution certain multiple), 120ul fluorescein in black enzyme mark 96 orifice plate, add 60ulAAPH after 37 DEG C of insulation 5min, make fluorescein and AAPH final concentration be respectively 70nM and 12mM, enter fluorescence microplate reader immediately and measure.Condition determination is that every 2min measures once, and continue 3h, excitation wavelength and emission wavelength are respectively 485nm, 530nm.With Trolox concentration for abscissa, light absorption value is that vertical coordinate makes standard curve, and according to standard curve calculation sample oxidation resistance, result represents (mmolTrolox/gDW) with Trolox equivalent in every gram of extract.
Result is as shown in table 1, and Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract prepared by embodiment 1,3,4 has stronger scavenging free radicals, antioxidative ability, and in being better than patent of invention [201310169455.1], embodiment 1 prepares gained Flos Osmanthi Fragrantis polyphenol extract.
Table 1 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract Scavenging ability
Note: Flos Osmanthi Fragrantis polyphenol extract is for preparing gained according to embodiment 1 in patent of invention [201310169455.1].
Embodiment 6, anti-aging effects are studied
The Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract that this experiment selects the present invention to prepare, adopts corn culture medium to raise fruit bat and the filling of preparation variable concentrations solution feeds mice two kinds of methods, to observe the antidotal effect of Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract.
1, Drosophila melanogaster is put into containing 0.2%(quality %) the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of embodiment 1,3,4 or the culture tube of Flos Osmanthi Fragrantis polyphenol extract preparation raise, and not add the culture tube of Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract for blank group, is divided into four groups.Each dosage group fruit bat about 200, male and female half and half, often group establish 4 parallel.Once, the mortality of statistics fruit bat and existence number, until all dead for the every day of each observation sooner or later.Respectively organize the length in life-span, record its half death time, maximum life span and average life.
The formula of above-mentioned culture medium is: water 76g, agar 1.5g, sugared 13.5g, Semen Maydis powder 10g, yeast powder 0.7g, propanoic acid 0.5mL.Preparation method is: get the half of answering amount of water, adds agar and boils stirring thawing, add white sugar; Get second half water by Semen Maydis powder furnishing pasty state, join stirring in agar water and be cooked into pasty state, add 0.5ml propanoic acid and stir, be distributed into culture bottle, about 2cm is high, beyond the Great Wall tampon; Autoclaving, 15 pounds/cm
2, 20 points of kinds, what add yeast and sterilizing after cooling perches paper.
2, select 60 healthy Kunming mouses, be divided into 6 groups at random: namely Normal group, model control group, Flos Osmanthi Fragrantis polyphenol extract (500mg/kg), implement 1, implement 3, implement 4 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extracts (500mg/kg).Modeling method adopts mice aging model, with the D-galactose solution of normal saline configuration 10%, except Normal group, all the other 4 groups of D-galactose solution of injecting 0.14g/kg respectively at neck dorsal sc every day make mice aging model, give D-galactose continuously 50 days.From second week, to the mice of administration group, distinguish Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract or the Flos Osmanthi Fragrantis polyphenol extract of gavage 500mg/kg every day; Blank group, model group control group mice gavage normal saline.The sign behavior of timing every day observed and recorded mice and active situation thereof, the body weight change of record mice, hair luster degree, situation of searching for food, sensitivity, mobbing reaction, drowsiness degree etc. to irritant reaction.After last administration 2h, take out brain, spleen, kidney, liver, suck blood, cut off fat, mesentery.By mouse spleen, after normal saline is cleaned, remove organ surface moisture with filter paper is sticky, weigh and calculate index and spleen index.Brain, spleen, kidney, liver are prepared into the tissue homogenate of 10%, by test kit illustration method, measure SOD and MDA content in the brain of mice, spleen, kidney, liver respectively.Experimental data adopt SPSS20.0 statistical software carry out variance analysis, result with
represent, adopt t to check between group and add up.
Through observation, record, after completing life span of drosophila melanogaster counting, statistical analysis is carried out to the experimental data of gained, result shows, compared with not dosing group in culture medium, the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of embodiment 1,3,4 and Flos Osmanthi Fragrantis polyphenol extract all have prolongation effect in various degree to the half survival time of fruit bat, average life and maximum life span.The average life of female male drosophila is extended 32.3%, 38.7%, 29.3% and 26.9%, 31.6%, 21.4% by the Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of embodiment 1,3,4 respectively, matched group is compared, extend successful, difference has statistical significance (P<0.05), and is better than Flos Osmanthi Fragrantis polyphenol extract (13.9% and 13.3%) and the results are shown in Table 2.
Table 2 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract is on the impact of life span of drosophila melanogaster
Note: compared with matched group, * P<0.05, * * P<0.01; Flos Osmanthi Fragrantis extract is for preparing gained according to embodiment 1 in patent of invention [201310169455.1].
The normal group mice mental status is good, physical agility, hair luster, not easily capture; Model group mice lassitude, be slow in action, hair color is withered does not revolt when doing not gloss, depilation, crawl, be easy to capture; The above-mentioned performance of administration group mice has optimum reverse compared with model group; Embodiment 1,3,4 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract doses group compared with normal group mice profile is unchanged, movable as usual.D-galactose model group mouse spleen index obviously declines, and has significant difference (P<0.05) compared with Normal group, illustrates that the mouse aging immunity that D-galactose model is induced obviously declines.After embodiment 1,3, the 4 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract of gavage 500mg/kg, compared with model group, index and spleen index obviously increases, and has significant difference (P<0.05), and is better than same dosage Flos Osmanthi Fragrantis polyphenol extract, the results are shown in Table.The SOD content of normal mouse tissues is higher, and the SOD content of mouse aging tissue is lower.Embodiment 1,3,4 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract can improve mouse brain, kidney, hepatic tissue SOD content, compared with model group, there is significant statistical significance (P<0.05 or P<0.01), be better than same dosage Flos Osmanthi Fragrantis polyphenol extract, the results are shown in Table.The MDA content of normal mouse tissues is lower, and the MDA content of mouse aging tissue is higher.Embodiment 1,3,4 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract can reduce mouse brain, kidney, Liver MDA, compared with model group, there is significant statistical significance (P<0.05 or P<0.01), be better than same dosage Flos Osmanthi Fragrantis polyphenol extract, the results are shown in following table.
Table 3 mouse spleen index
Note: compared with model group, * P<0.05, * * P<0.01; Flos Osmanthi Fragrantis polyphenol extract is for preparing gained according to embodiment 1 in patent of invention [201310169455.1].
The sweet extract of table 4 Flos Osmanthi Fragrantis phenethanol is on the impact of D-gal Aging model SOD content
Note: compared with model group, * P<0.05, * * P<0.01; Flos Osmanthi Fragrantis polyphenol extract is for preparing gained according to embodiment 1 in patent of invention [201310169455.1].
The sweet extract of table 5 Flos Osmanthi Fragrantis phenethanol is on the impact of D-gal Aging model M DA content
Note: compared with model group, * P<0.05, * * P<0.01; Flos Osmanthi Fragrantis polyphenol extract is for preparing gained according to embodiment 1 in patent of invention [201310169455.1].
This experiment is first tested by drosophila survival, tentatively shows that Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract has certain anti-aging effects, and inquires into it further to the anti-aging effects of D-gal Aging mouse model.Adopt the classical way of injection of d-galactose to make aging model in experiment, long term injections D-galactose can cause general metabolism disorder, immune organ is degenerated, free radical is superfluous.Experimental result shows, there is obvious old and feeble sign behavior in model mice, such as lassitude, be slow in action, the withered original text of hair color not gloss, be easy to capture etc., there is atrophy in spleen, in brain, kidney, liver, SOD content declines, MDA content increases, and shows that it is successful for making aging model through the injection of D-galactose.Existing known by free radical cause aging, not only threaten the life-span of people, also with the disease relevant with aging, such as senile dementia, cancer, cardiovascular and cerebrovascular disease etc., even patient's rejuvenation day by day, this becomes the formidable enemy of human health and the heavy burden of social prosperity gradually.Under normal circumstances, the generation of machine interior free yl keeps balancing with elimination, but with advancing age or the impact of disease, in body, the activity of antioxidase weakens gradually, can not scavenging free radicals and cause the excessive generation of lipid peroxide in time, make membrane structure and the function generation obstacle of cell, cause aging and the death of cell.SOD is the important antioxidase of scavenging free radicals in body, its content high mental retardation direct reaction goes out the ability of body scavenging free radicals, and MDA is the catabolite of lipid peroxide, increases with the age and be proportionate, the order of severity that its content high mental retardation indirect reaction body cell is attacked by free radical.This experimentation shows, creates oxidative damage in D-gal Aging model mice body, and the body accumulation amount of lipid peroxide increases.Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract can have facilitation to the activity of SOD in exhausted mining areas body, MDA content can be suppressed to increase simultaneously, illustrate that aging resistance effect is relevant with the activity of its raising antioxidase and the generation of anti-lipid peroxidation thing etc.Experimental data shows, and Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract improves mouse aging hepatic tissue SOD content, reduces the effect of MDA content the most obviously, illustrates that Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract protects hepatocellular effect the most obvious.Lipid peroxide major part is produced by hepatocyte, and its decomposition is also main at liver, and MDA is the main degradation products of lipid peroxide.This experiment also shows that Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract can improve the spleen index of mouse aging, obviously can suppress the mice atrophy that spleen occurs in aging course, has the effect of protection immune organ, enhancing immunity.This shows, Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract can improve body immunity, oxygen-derived free radicals in purged body, anti-lipid peroxidation, can defying age, has preventive and therapeutic effect to the disease relevant with free radical.
Embodiment 7, D-galactose is caused to the impact of subacute aging ability of learning and memory in mice
This experiment studies the antidotal effect of Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract based on the improvement of ability of learning and memory.Select Kunming mouse 72, body weight 18 ~ 22 grams, male and female half and half, before grouping, a Morris water maze orientation navigation experiment is carried out to all mices, find time (incubation period) and the body weight of platform according to mice, 72 mices are divided into six groups: blank group, aging model group, embodiment 1, embodiment 3, embodiment 4 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, Flos Osmanthi Fragrantis polyphenol extract, often organize 12.Model group and administration group mouse carotid dorsal sc injection 10%D-galactose 0.lmL/10gd (normal saline), continuous 51 days, matched group injection normal saline; Experiment second week, the embodiment 1,3,4 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract solution 0.125mL/10gd (normal saline) of administration group mice timing every day gavage 8% concentration, continuous 44 days, matched group and model group mouse stomach normal saline.
Morris water maze: (1) orientation navigation experiment.Morris water maze is primarily of round pool, removable cylindrical transparent Plexiglas platform, automatic production record composition.Round pool diameter 80cm is highly 50cm, water temperature 25 ± 2 DEG C, and pond is divided into four quadrants.Platform diameter 5cm, height 18cm, be placed in third quadrant, and be positioned at the center of circle and pool wall line center, in experimentation, position remains unchanged, lcm in underwater.Test the 45th day, train after injected in mice, gavage, the upper and lower noon is each once, lasts 5 days.By mice towards pool wall, be fixed into water spot from first quartile and put into water gently, record its time finding platform in 120s (incubation period), find platform relief mice to stop 20s on platform.If mice fails to find platform in 120s, then caused platform, be allowed to condition on platform simultaneously and stop 20s, then put back in cage, incubation period is designated as 120s.Test first four days for training period, be not counted in statistical analysis.Test the 5th day, above, afternoon, twice preclinical arithmetic equal value was as school grade.(2) space exploration experiment.Orientation navigation experiment terminates latter 24 hours, shifts out platform, is still fixed into water spot with first quartile and mice is put into water gently towards pool wall, the number of times measuring mice spanning platform relevant position in 120s and the time stopped at platform place quadrant.
Jumping test: mice diving tower instrument is the passive avoidance trained reflex case that 32cm*22.5cm*33cm is transparent, is divided between six with opaque black plastic plate, is made up of the thin copper grid of several spacing 0.5cm at the bottom of case.Left rear corner between every placed a high 4.5cm, the cylindrical valve rubber of diameter 4.5cm.During experiment, by mice in reaction chamber, adapt to 3min, then logical 24V alternating current immediately, mice is shocked by electricity, its normal reaction be rebound valve rubber to hide electric shock, energising 5min.First day is diving tower training, and record mice skips to the number of times (errors number) of copper grid in 5min from platform, as mice school grade.After 24h, cyclical test, record mice first time jumps off the errors number in time (incubation period) of platform and 5min, as Memory result.If mice does not jump off platform in 5min, be then designated as 300s incubation period, errors number is designated as 0 time.This experiment is carried out after space search experiment terminates.Due to aging model group have 3 mices, low dose group has 2 mice behaviors too slow, be unfavorable for carrying out Behavior test, therefore these 5 mices do not take one's test in an experiment.
The data obtained mean ± standard deviation (X ± S) represents, carries out one factor analysis of variance with SPSS17.0 statistical software.
Experimental result shows: there is some difference incubation period for each group, D-galactose Aging mice orientation navigation ability reduces, embodiment 1,3,4 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract has certain improvement result to the ability of learning and memory of mouse aging in orientation navigation experiment, all there were significant differences compared with model group (P<0.01 or P<0.05), and be better than the Flos Osmanthi Fragrantis polyphenol extract of same dosage, the results are shown in following table.
The sweet extract of table 6 Flos Osmanthi Fragrantis phenethanol is on the impact of the little genus orientation navigation experiment of subacute aging
Note: compared with model group, * P<0.05, * * P<0.01; Flos Osmanthi Fragrantis polyphenol extract is for preparing gained according to embodiment 1 in patent of invention [201310169455.1].
Experimental result shows: Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract has certain improvement result for the memory ability of subacute aging mice in the space search time.Embodiment 1,3,4 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract can significantly improve the number of times that subacute aging mice passes through original platform, improve and stop original platform quadrant time, compared with model group, all there were significant differences (P<0.01 or P<0.05), and be better than the Flos Osmanthi Fragrantis polyphenol extract of same dosage, the results are shown in following table.
The impact that table 7 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract is tested the space exploration of subacute aging mice
Note: compared with model group, * P<0.05, * * P<0.01; Flos Osmanthi Fragrantis polyphenol extract is for preparing gained according to embodiment 1 in patent of invention [201310169455.1].
Experimental result shows: the ability of learning and memory of Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract to D-galactose Aging mice has certain improvement result.The test of first day errors number represents the learning capacity of mice, model group there are differences compared with blank group, there is statistical significance (P<0.05), embodiment 1,3,4 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract can significantly reduce first day errors number, compared with model group, all there were significant differences (P>0.05), and be better than the Flos Osmanthi Fragrantis polyphenol extract of same dosage.Second day errors number and second day incubation period represent the memory ability of mice, significant difference (P<0.01) is there is in model group compared with blank group, embodiment 1,3,4 Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract significantly can reduce by second day errors number, increase by second day incubation period, compared with model group, all there were significant differences (P>0.05), and be better than the Flos Osmanthi Fragrantis polyphenol extract of same dosage, the results are shown in following table 8.
The sweet extract of table 8 Flos Osmanthi Fragrantis phenethanol is on the impact of subacute aging mice Jumping test
Note: compared with model group, * P<0.05, * * P<0.01; Flos Osmanthi Fragrantis polyphenol extract is for preparing gained according to embodiment 1 in patent of invention [201310169455.1].
This experiment adopts the method for neck dorsal sc injection D-galactose to build subacute aging mouse model, finds that the ability of learning and memory of model group mice obviously declines by Behavior test.Simultaneously, experimental result shows: administration group is after gavage gives different Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract solutions, in Morris water maze laboratory, shorten the incubation period finding platform to some extent, add cross over platform number of times and in the original platform quadrant time of staying; In mice Jumping test, first day, second day errors number reduce to some extent, and second day incubation period extended to some extent.Experimental result demonstrates administration group has raising without degree in identification learning ability and dependent response ability.Experiment prompting: Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract has and necessarily improves the effect that D-galactose causes subacute aging model ability of learning and memory in mice.In sum, the present invention is tested by drosophila survival, proves that Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract has certain anti-aging effects, has certain improvement result to the ability of learning and memory of D-galactose Aging mice, and drug effect is clear and definite, provides a kind of selection newly for clinical.
Finally, it is also to be noted that what enumerate above is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.
Claims (3)
1. Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract is preparing the application in antiaging agent or health product, it is characterized in that: in Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract, phenylethanoid glycosides content is 30 ~ 90%, and described phenylethanoid glycosides comprises verbascoside, different verbascoside; In phenylethanoid glycosides, verbascoside is 48:1 or 77:1 with the ratio of the weight of different verbascoside.
2. Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract according to claim 1 is preparing the application in antiaging agent or health product, it is characterized in that preparation method comprises the following steps:
(a), the Osmanthus tragranscv.Aurantiacu dry flower of 1kg moisture≤10% is crushed to and can crosses 20 objects sieves, being added to 10L volumetric concentration is in the ethanol water of 90%, add AOB solution 2L again, utilizing 1mol/L acetic acid aqueous solution to adjust pH is 5, obtains extraction solution;
Containing 1g AOB in often liter of AOB solution;
(b), extraction solution is carried out lixiviate 2 hours at 50 ~ 80 DEG C, after being cooled to room temperature, filter or centrifugal, obtain extracting solution;
(c), extracting solution is reclaimed ethanol through concentrating under reduced pressure, obtain concentrated solution; Dilute with water again, until the pH of gained diluent is 4;
(d), diluent is adsorbed through adsorbent resin, be the ethanol water eluting of 30% by volumetric concentration;
E, after (), eluent concentrating under reduced pressure, spray-dried, low pressure is dried or microwave drying, obtains Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract.
3. Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract according to claim 1 is preparing the application in antiaging agent or health product, it is characterized in that preparation method comprises the following steps:
(a), the Osmanthus fragrans var.thunbergii. dry flower fragmentation of 1kg moisture content≤10% is crushed to and can crosses 20 objects sieves, being added to 15L volumetric concentration is in 80% ethanol water, add arabo-ascorbic acid sodium water solution 2L again, utilizing the adjustment of 1mol/L aqueous formic acid to extract pH value of solution is 5, obtains extraction solution;
Containing 1g sodium erythorbate in often liter of arabo-ascorbic acid sodium water solution;
(b), extraction solution is carried out lixiviate 2 hours at 50 ~ 80 DEG C, after being cooled to room temperature, filter or centrifugal, obtain extracting solution;
(c), extracting solution is reclaimed ethanol through concentrating under reduced pressure, obtain concentrated solution; Dilute with water again, until the pH of gained diluent is 5;
(d), diluent is adsorbed through adsorbent resin, be the ethanol water eluting of 30% by volumetric concentration;
E, after (), eluent concentrating under reduced pressure, spray-dried, low pressure is dried or microwave drying, obtains Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract.
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