CN104042499B - The preparation method of gold sweet osmanthus active principle and purposes - Google Patents
The preparation method of gold sweet osmanthus active principle and purposes Download PDFInfo
- Publication number
- CN104042499B CN104042499B CN201410251138.9A CN201410251138A CN104042499B CN 104042499 B CN104042499 B CN 104042499B CN 201410251138 A CN201410251138 A CN 201410251138A CN 104042499 B CN104042499 B CN 104042499B
- Authority
- CN
- China
- Prior art keywords
- sweet osmanthus
- concentrate
- active principle
- gold
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Cosmetics (AREA)
Abstract
The present invention relates to preparation method and the purposes of a kind of gold sweet osmanthus active principle, belong to traditional Chinese medicine effective ingredient extraction field.Its preparation process comprises the following steps: a, take gold sweet osmanthus, add alcohol extract, filter, concentrate, obtain concentrate；B, described concentrate is cooled to room temperature, adds the ethanol that mass percent concentration is 80%～95% and carry out alcohol precipitation, stand, filter, concentrate, obtain golden sweet osmanthus active principle.The invention has the beneficial effects as follows: this preparation method simple possible, the active component in gold sweet osmanthus can be effectively retained.This active principle use safety, action temperature and, have anti-oxidant, anti-aging, protection skin, delay skin aging isoreactivity, may be used for the additive of food, health products, cosmetics and medicine or as raw material.
Description
Technical field
The invention belongs to traditional Chinese medicine effective ingredient extraction field, be specifically related to a kind of gold sweet osmanthus active principle preparation method with
Purposes.
Background technology
Gold sweet osmanthus is evergreen shrubs or dungarunga section, and leaf is to life, and how in oval or oblong, leaf blade face is smooth, leather
Matter, limb edge has sawtooth, and flowers are raw.Flower taste is pungent, can be used as medicine.Have reduce phlegm, cough-relieving, promote the production of body fluid, the effect such as pain relieving.Owing to its taste is fragrant,
Persistently, it is usually used in making cake, candy etc..
But, the research to gold sweet osmanthus now rarely has report, greatly constrains the application of gold sweet osmanthus.
Summary of the invention
It is an object of the invention to provide preparation method and the purposes of a kind of gold sweet osmanthus active principle.
The preparation method of a kind of gold sweet osmanthus active principle of the present invention, comprises the following steps:
A, take gold sweet osmanthus, add alcohol extract, filter, concentrate, obtain concentrate；
B, described concentrate is cooled to room temperature, adds the ethanol that mass percent concentration is 80%～95% and carry out alcohol
Heavy, stand, filter, concentrate, obtain golden sweet osmanthus active principle.
Preferably, the ethanol mass percent concentration being used in described step a extracting is 70%～90%.
Preferably, the number of times extracted in described step a is 2～5 times, and the time of extraction is 1～3 hour.It is particularly preferred,
The number of times extracted is 3 times, 4 times；The time extracted is 2 hours.
Preferably, described step a adds 8～20 times that consumption is described gold sweet osmanthus weight of alcohol extract.
Preferably, the 60% of the weight of concentrate in described step a >=described gold sweet osmanthus weight.
Preferably, for 3～6 times that ethanol consumption is described concentrate weight of alcohol precipitation in described step b.
Preferably, the time stood in described step b is 4～10 hours；Particularly preferred, the time of standing is 5 hours,
6 hours, 7 hours, 8 hours and 9 hours.
The present invention also proposes the golden sweet osmanthus active principle that a kind of described preparation method prepares.
The invention allows for the sweet osmanthus active principle application in preparing antioxidation cosmetic product of a kind of gold.
The invention allows for a kind of described golden sweet osmanthus active principle, this gold sweet osmanthus active principle comprises benzene oxygen second
Alcohol.The addition of its Phenoxyethanol is the 0.1%～1% of whole gold sweet osmanthus active principle weight.
In the present invention, with above-mentioned cosmetics or direct composition based on gold sweet osmanthus active principle, then can be equipped with other
Supplementary material, makes cosmetics finished product according to the common process preparing cosmetics.
The beneficial effects of the present invention is: with gold sweet osmanthus as raw material, work out a kind of system preparing gold sweet osmanthus active principle
Preparation Method.This preparation method simple possible, can be effectively retained the general flavone isoreactivity composition in gold sweet osmanthus.This active principle uses
Safety, action temperature and, have anti-oxidant, anti-aging, protection skin, delay skin aging isoreactivity, may be used for food, health care
The additive of product, cosmetics and medicine or as raw material.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, embodiment will be described below
The accompanying drawing used required in is briefly described.
Fig. 1 is the gold sweet osmanthus active principle test figure to DPPH radicals scavenging effect；
Fig. 2 is the gold sweet osmanthus active principle test figure to the scavenging action of superoxide anion.
Detailed description of the invention
For making those skilled in the art understand production technology and the technique effect of the present invention in detail, below with concrete production
Example is further described application and the technique effect of the present invention.
Embodiment 1:
A, take gold sweet osmanthus, add 15 times amount mass percent concentrations and be 75% alcohol extract 3 times, extract 2 hours, mistake every time
Filter, concentrates, obtains concentrate；Wherein, the weight of gained concentrate is the 60% of gold sweet osmanthus weight；
B, described concentrate is cooled to room temperature, on the basis of the weight of concentrate, adds 4 times amount mass percent concentrations
Be 85% ethanol carry out alcohol precipitation, stand 6 hours, filter, concentrate, obtain golden sweet osmanthus active principle.
Embodiment 2:
A, take gold sweet osmanthus, add 20 times amount mass percent concentrations and be 75% alcohol extract 2 times, extract 1 hour, mistake every time
Filter, concentrates, obtains concentrate；Wherein, the weight of gained concentrate is the 55% of gold sweet osmanthus weight；
B, described concentrate is cooled to room temperature, on the basis of the weight of concentrate, adds 4 times amount mass percent concentrations
Be 80% ethanol carry out alcohol precipitation, stand 8 hours, filter, concentrate, obtain golden sweet osmanthus active principle.Wherein, effective this gold sweet osmanthus
Adding Phenoxyethanol in component, the addition of Phenoxyethanol is the 0.5% of whole gold sweet osmanthus active principle weight.
Embodiment 3:
A, take gold sweet osmanthus, add 8 times amount mass percent concentrations and be 70% alcohol extract 3 times, extract 3 hours, mistake every time
Filter, concentrates, obtains concentrate；Wherein, the weight of gained concentrate is the 58% of gold sweet osmanthus weight；
B, described concentrate is cooled to room temperature, on the basis of the weight of concentrate, adds 6 times amount mass percent concentrations
Be 70% ethanol carry out alcohol precipitation, stand 4 hours, filter, concentrate, obtain golden sweet osmanthus active principle.At this gold sweet osmanthus active principle
Middle interpolation Phenoxyethanol, the addition of Phenoxyethanol is the 0.1% of whole gold sweet osmanthus active principle weight.
Embodiment 4:
A, take gold sweet osmanthus, add 10 times amount mass percent concentrations and be 90% alcohol extract 2 times, extract 2 hours, mistake every time
Filter, concentrates, obtains concentrate；Wherein, the weight of gained concentrate is the 54% of gold sweet osmanthus weight；
B, described concentrate is cooled to room temperature, on the basis of the weight of concentrate, adds 3 times amount mass percent concentrations
Be 90% ethanol carry out alcohol precipitation, stand 10 hours, filter, concentrate, obtain golden sweet osmanthus active principle.At this gold sweet osmanthus active principle
Middle interpolation Phenoxyethanol, the addition of Phenoxyethanol is the 1% of whole gold sweet osmanthus active principle weight.
Comparative example 1:
Taking gold sweet osmanthus 10 times amount soak by water 2 times, the time every time decocted is 2 hours, obtains golden sweet osmanthus water extract.
Comparative example 2:
Take the gold sweet osmanthus alcohol extract that 10 times amount mass percent concentrations are 50% 2 times, extract 2 hours every time, obtain golden
Sweet osmanthus alcohol extracting thing.
Comparative example 3:
Take the gold sweet osmanthus alcohol extract that 10 times amount mass percent concentrations are 90% 2 times, extract 2 hours every time, obtain golden
Sweet osmanthus alcohol extracting thing.
For verifying effect of the present invention, spy makees comparative testing below:
| Product | General flavone |
| Embodiment 1 | 13.5% |
| Embodiment 2 | 12.8% |
| Embodiment 3 | 13.2% |
| Embodiment 4 | 12.5% |
| Comparative example 1 | 3.7% |
| Comparative example 2 | 4.6% |
| Comparative example 3 | 4.7% |
As seen from the above table, by the golden sweet osmanthus active principle of preparation method gained of the present invention, compare comparative example 1-3, always yellow
Ketone content is higher, the active component that can be effectively retained in gold sweet osmanthus is described, achieves significant technological progress.
Another embodiment 1-4 and comparative example 1-3 gained active principle and the extract of passing through is to DPPH radicals scavenging effect
Discovery is compared in research, and in embodiment, the oxidation resistance of the golden sweet osmanthus active principle of gained is 3-extract obtained in comparative example
6 times.
For verifying the technique effect of the present invention, spy makees tests below:
1, the gold sweet osmanthus active principle test to DPPH radicals scavenging effect
Experimental principle: DPPH methanol solution is pansy, has strong light absorption value under 517nm, if being combined with sample, and meeting
Reduce light absorption value, thus remove the ability of free radical so as to judgement sample.
Experimental technique
Experimental group: take 0.1mL, 0.2mL, 0.5mL, 1mL gold sweet osmanthus active principle solution (concentration is 6.7mg/mL) respectively
Sample, in test tube, supplies 1mL with distilled water, adds 4mLDPPH methanol solution, mixing, and dark place is placed 30min, constantly shaken
Swing, measure the light absorption value at 517nm.
Blank group: take the sample solution of same experimental group equal volume in each test tube respectively, supply with distilled water
1mL, adds the methanol solution of 4mL.After mixing, 30min is placed in dark place, measures light absorption value at 517nm.
Control group: 1mL distilled water adds 4mLDPPH methanol solution.After mixing, 30min is placed in dark place, measures at 517nm
Light absorption value.
Experimental result: DPPH inhibiting rate (%)=(A0-A1+A2)/A0*100%
Wherein, A0 is the light absorption value of control group, and A1 is the light absorption value of experimental group, and A2 is the light absorption value of corresponding blank group.
Result is shown in Fig. 1；Gold sweet osmanthus active principle can effectively remove DPPH free radical, and all reaches in test scope clearance rate
To more than 95%.
2, the scavenging action of superoxide anion is tested by gold sweet osmanthus active principle
Experimental principle: pyrogallol autoxidation in weakly alkaline environment decomposes generation superoxide anion, along with reaction
Carrying out, superoxide anion constantly accumulates in system, causes reactant liquor absorbance at 420nm to start a period of time in reaction
Interior meeting change over time and linearly increase, therefore measure within this period absorbance of measured object reactant liquor in time
Rate of change, and the ability that just can draw measured object suppression superoxide anion is compared with blank solution.
Experimental technique
Experimental group: take 5.6mLTris HCl buffer solution, is separately added into the golden sweet osmanthus of 0.1mL, 0.2mL, 0.3mL, 0.4mL
Active principle solution (concentration is 6.7mg/mL) sample, supplies 0.4mL with distilled water, puts into water-bath in 25 DEG C of water-baths
10min, is subsequently adding the pyrogallol (if room temperature can at room temperature operate higher than 25 DEG C) of 25 DEG C of pre-temperature, fully mixes,
After adding pyrogallol, start accurate timing 3min, then add 5% ascorbic acid (Vc) 0.05mL and terminate reaction.10min
At 420nm, its absorbance is measured after clock.
Control group: take the Tris HCl buffer solution of 5.6mL, is separately added into effective group of the golden sweet osmanthus of same experimental group same volume
Divide solution (concentration is 6.7mg/mL), supply 0.4mL with distilled water, add 12mmol/LHCl, fully mix, from adding HCl
Start accurate timing 3min, add 5% ascorbic acid and terminate reaction.At 420nm, its absorbance is measured after 10min clock.
Blank group: take 5.6mL50mmol/L Tris-HCl buffer solution, adds 0.4mL distilled water, and hereafter operation is with experiment
Group.
Experimental result: E=[(A0-A1+A2)/A0] * 100%
A0 is Blank absorbance values, and A1 is experimental group absorbance, and A2 is control group absorbance.
Result is shown in Fig. 2；Gold sweet osmanthus active principle shows certain effect removing superoxide anion.
Finally it should be noted that above example only in order to illustrative not limiting technical scheme, although reference
The present invention has been described in detail by above-described embodiment, it will be appreciated by those skilled in the art that and still can carry out the present invention
Amendment or equivalent, any modification or partial replacement without departing from the spirit and scope of the present invention, it all should be contained
In scope of the presently claimed invention.
Claims (3)
1. the preparation method of a golden sweet osmanthus active principle, it is characterised in that comprise the following steps:
A, take gold sweet osmanthus, add alcohol extract, filter, concentrate, obtain concentrate；
B, described concentrate is cooled to room temperature, adds the ethanol that mass percent concentration is 80%～95% and carry out alcohol precipitation, quiet
Put 4～10 hours, filter, concentrate, obtain golden sweet osmanthus active principle；
Wherein, in described step a, it is 70%～90% for the ethanol mass percent concentration extracted；Extract number of times be 2～
5 times, the time of extraction is 1～3 hour；Add 8～20 times that consumption is described gold sweet osmanthus weight of alcohol extract；Concentrate
The 60% of weight >=described gold sweet osmanthus weight；
In described step b, the ethanol consumption for alcohol precipitation is 3～6 times of described concentrate weight.
2. the golden sweet osmanthus active principle that a preparation method according to claim 1 prepares.
Gold the most according to claim 2 sweet osmanthus active principle application in preparing antioxidation cosmetic product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410251138.9A CN104042499B (en) | 2014-06-09 | 2014-06-09 | The preparation method of gold sweet osmanthus active principle and purposes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410251138.9A CN104042499B (en) | 2014-06-09 | 2014-06-09 | The preparation method of gold sweet osmanthus active principle and purposes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104042499A CN104042499A (en) | 2014-09-17 |
| CN104042499B true CN104042499B (en) | 2016-08-24 |
Family
ID=51496231
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410251138.9A Active CN104042499B (en) | 2014-06-09 | 2014-06-09 | The preparation method of gold sweet osmanthus active principle and purposes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104042499B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111887469A (en) * | 2019-05-05 | 2020-11-06 | 贵州中烟工业有限责任公司 | Preparation method of burley tobacco additive |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI411440B (en) * | 2011-06-02 | 2013-10-11 | Chien Ya Hung | Use of sweet tea olive extract for prevention or treatment of melancholia |
| CN103768151B (en) * | 2014-01-29 | 2016-03-16 | 浙江大学 | Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract is preparing the application in antiaging agent or health product |
-
2014
- 2014-06-09 CN CN201410251138.9A patent/CN104042499B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104042499A (en) | 2014-09-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104666237B (en) | The preparation method and purposes of truffle active principle | |
| CN104983632B (en) | Plant composition for skin care and cosmetics with moisturizing and improvement skin elasticity effect | |
| CN105147550B (en) | A kind of whitening moisturizing face masque liquid containing nostoc sphaeroids kutz extractive and preparation method thereof | |
| CN107362084A (en) | A kind of facial mask containing Chinese sumac leaf extract and preparation method thereof | |
| CN101507698A (en) | Composite with whitening function and skin care product thereof and preparation method thereof | |
| CN102940597A (en) | Natural compound botanical antioxidant, as well as preparation method and application thereof in cosmetics | |
| CN107034022A (en) | A kind of preparation technology of anti-oxidant arborvitae essential oil highly finished product | |
| CN111514072B (en) | Millettia speciosa-noni composite enzyme, preparation method thereof and application of millettia speciosa-noni composite enzyme in preparation of facial mask | |
| JP2011088845A (en) | Involucrin expression inhibitor | |
| KR101921086B1 (en) | A cosmetic composition for blood circulation and improving skin tone comprising hydrogen water and camucamu extract | |
| IT8222578A1 (en) | HAIR CARE PRODUCT | |
| CN104042499B (en) | The preparation method of gold sweet osmanthus active principle and purposes | |
| CN107929131B (en) | Anti-aging composition and application thereof | |
| CN103550101A (en) | Plant extract with whitening and moisturizing functions and application thereof | |
| KR20130079220A (en) | Skin external composition containing extract of soybean root | |
| CN108721192A (en) | A kind of extracted form natural plant essence facial mask liquid and its extraction process | |
| CN110200880B (en) | Composition and application thereof in preparing antioxidant product | |
| CN109846967B (en) | Traditional Chinese medicine composition with antibacterial, itching-relieving and antioxidant effects | |
| CN105232776A (en) | Oral preparation for whitening, moisturizing and skin caring | |
| CN107669525B (en) | Application of Poria extract with whitening effect and its preparation method | |
| KR100893622B1 (en) | Soap and it's making method for smell clearance using salicornia herbacea and white lotus | |
| CN108066189A (en) | There is the anti-ageing ganoderma lucidum skin care compositions for dispelling yellow and its application | |
| CN112675080A (en) | Skin care emulsion containing hemerocallis fulva flower polysaccharide and preparation method thereof | |
| CN103948670A (en) | Preparation method and applications of mung bean peel effective component | |
| CN110693791A (en) | Preparation of pure dew yeast essence toner (toning lotion) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |