KR101519234B1 - Cosmetic composition containing curcuma extracts and camellia leaf extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a turmeric (Curcuma longa) extract and camellia (Camellia japonica) leaf extract. More specifically, the cosmetic composition contains the turmeric extract and camellia leaf extract in a weight ratio of 10:3 to 10:10. Even though the high cohesion and viscosity of the turmeric extract makes the turmeric extract hard to be formulated as a cosmetic composition, the present invention mixes the turmeric extract with the camellia leaf extract to address the difficulty, thereby providing the cosmetic composition with excellent anti-oxidizing and anti-microbial capabilities.

Description

The present invention relates to cosmetic composition containing curcuma extract and camellia leaf extracts,

More particularly, the present invention relates to a cosmetic composition comprising a herbal composition and a camphor leaf extract in a weight ratio ranging from 10: 3 to 10, and in the case of a herbal composition, a cohesive force and a viscosity Can hardly be formulated into cosmetics. However, such problems can be solved by mixing camellia sinensis leaf extract with this, and a cosmetic composition having excellent antioxidative, antimicrobial, deodorizing ability and atopic skin improving ability can be provided.

In general, the soap functions as a detergent for removing various bacteria and the like which are contaminated from waste products on the body skin, keratin and external contact in the process of washing water, washing the hair, bathing and the like. In addition, the soap is used not only for cleaning the skin but also for washing laundry for washing various clothes. In general, beauty soap is prepared by mixing a proper amount of oil (eg, woji, lard, sunshine) with palm oil, heating and dissolving at 60 to 70 ° C., adding sodium hydroxide (NaOH) to saponification reaction, Water, unreacted sodium hydroxide, and a glycerin layer, which are by-products of the saponification reaction, are separated and then subjected to 1-2 times of concentrated salt water to remove residual glycerin and sodium hydroxide in the soap to obtain a conventional soap layer, Thereby producing a soap having a moisture content of about 13%. A small amount of an antioxidant, a complementary coloring agent, a fatty acid flavoring agent, a vegetable flavoring agent or the like is blended with the thus-produced soap to form and manufacture it.

The conventional cosmetic soap has the advantage of being able to maintain the cleanliness of the skin by removing waste products generated from the skin, cosmetics remaining on the skin, and various foreign substances. However, since the anaerobic microorganisms Which is one of the inflammatory skin diseases, that has acne skin and atopic skin which complains of itching, as well as the disadvantage of not being able to kill and clean the skin (bacteria), viruses and small mites of less than 1 mm, In the case of humans, the use of conventional soap has been problematic in that the symptoms become worse.

In order to solve the problems of the conventional cosmetic soap, a variety of functional soaps having functional properties corresponding to various skin diseases have been proposed. These include soap bases 94 - 96%, herbal ingredients 1 - 3%, oil 1 %, Aroma oil 1%, glycerin 1%, and the like, followed by the same process as that of a conventional soap manufacturing method.

Curcuma longa L. is root of ginger and Zingiberaceae. Ginger (Zingiberaceae), the whole plant turmeric (Curcuma Wenyujin YH Chen et C. Ling), Curcuma longa L., Curcuma kwangsiensis SG Lee et CF Liang) or Curcuma phaeocaulis Val.). Ulgum is a perennial plant that grows in the tropics. The leaves are elliptical and 4 to 5 are gathered. Root stalks are in the shape of a cuspid or branched round pole and the folded side is dark blue. It grows in various places except the northern mountainous part of Korea. Flowers are yellowish white cylinders and flowering period is from August to November. The roots are washed in water and dried or steamed in the sun. Curcuma longa L.) Curcumin has been reported to have excellent antioxidant properties and is known to be converted naturally into a more potent antioxidant substance called tetrahydrocurcumin in the body.

Camellia japonica L.) belongs to the genus Chlorophyllum, and is an evergreen broad-leaved arboreous tree that grows in Jeonbuk, Gochang, Jeonnam, Haenam, Wando, Gangjin, Yeosu, Gwangyang, Gyeongnam, Geoje, South coast and Jeju Island. The key is about 15 m in diameter and about 50 ㎝ in diameter. The surface of the leaves is dark green and lustrous. The back side is yellowish green and oval. The length is 5 ~ 12 ㎝, width is 3 ~ 7 ㎝ and wavy sawtooth . Much research has been conducted on the analysis and application of components in camellia. Acute testing of camellia oil and oilseed rape showed that the ingredients contained in the oilseed rape inhibited the absorption of alcohol, and hyperoside isolated from the seedling showed HIV-1 protease related to AIDS induction HIV-1 protease), anti-inflammatory and anti-inflammatory activity of extracts of seed and peel, and methanol extract of stem inhibited lipid and oxidation. In addition, proline and glutamic acid are present as main amino acids in Camellia sinensis, and other small amounts of free amino acids are found. However, camellia leaves are not known much.

It can help prevent skin aging, moisturizing effect, removal of fine heavy metals and sebum in pores, while relieving symptoms without side effects even if it is used for a long time for various skin diseases including increasingly increasing dermatitis patients with environmental pollution. A functional soap composition, especially a functional soap composition, is desperately required.

In the case of Ulugum, it is not suitable for the production of cosmetics, because the Ulugum extract has a strong coagulation property and is difficult to be formulated because it does not spread homogeneously when mixed with other raw materials. Accordingly, it is an object of the present invention to provide a cosmetic composition which is excellent in antioxidant and antimicrobial activity, and easy to be formulated into cosmetics.

The present invention relates to a cosmetic composition containing urogine and camellia leaf extract, and more specifically, it relates to a cosmetic composition comprising a herbal composition and a camphor leaf extract in a weight ratio of 10: 3 to 10, The present invention can provide a cosmetic composition in which each component is evenly dispersed while containing the extract of Ulvae, which is difficult to formulate, and can provide a cosmetic composition having excellent antioxidative and antimicrobial properties.

It is possible to obtain a cosmetic composition in which each component is evenly dispersed by incorporating a specific component of Camellia sinensis leaf extract in addition to a herbal extract which is difficult to be formulated into cosmetics because of its strong cohesion and viscosity and also has antioxidative, antimicrobial, , A cosmetic composition having excellent atopic skin-improving ability can be provided.

FIG. 1 shows the results of visual observation of the dispersion state of the cosmetic composition blended with the extract of Eelgum and Camelliaceae according to the present invention.
2 is a graph showing the results of measuring the DPPH radical scavenging ability of the camphor leaf extract.
FIG. 3 is a graph showing the results of comparing antioxidative activities of leaves and stem.
4 is a graph comparing the antioxidative activity of Camellia sinica leaf extract with that of green tea leaf, showing the results of measurement of catalase activity for camellia lily, Camellia sinensis leaf extract showed higher catalase activity.
FIG. 5 is a graph showing an ammonia gas concentration curve over time, and shows a deodorizing test result of a composition containing an uroguard extract.
6 shows the results of an experiment on the antimicrobial effect of the extracts of A. tumefaciens ( S. epidermidis , B. M. furfur , C. P. ances ; No 1. 5 mg / ml, No 2. 3 mg / ml, No 3. 1 mg / ml)
FIG. 7 shows the results of an experiment on the antibacterial effect of M. tuberculosis extract against M. furfur. (a) P. ances , b. S. epidermidis , M. furfur, No 1. 30 μl of essential oil, No. 2. 20 μl of essential oil + 10 μl of EtOH, No. 3. 10 μl of essential oil + 20 μl of EtOH)
FIG. 8 is a graph showing the results of measuring the DPPH radical scavenging ability of the uroguin extract. FIG.
FIG. 9 is a graph showing the results of measurement of catalase activity of five herbal medicine extracts including herbal extracts.
FIG. 10 is a graph showing the results of ascorbate peroxidase (APX) activity measurement of five herbal medicine extracts containing the extracts of Ullungium bulb.
FIG. 11 is a graph showing the results of measurement of superoxide dismutase activity of five herbal medicine extracts containing the extracts of Euglena sp.

The present invention provides a cosmetic composition containing koi and camphor leaf extract.

In one embodiment according to the present invention, the wool and camphor leaf extract may be blended in a weight ratio range of 10: 3 to 10, specifically 10: 3 to 5, more specifically 10: 4 To 5 by weight. However, the lower limit value is more important since the coagulation phenomenon of the extract is not observed when the blend weight ratio of uroguin extract and camphor leaf extract is 10: 3 or more.

In one embodiment according to the present invention, the extract is obtained by mixing the herbaceous and camphor leaves with water, a C ₁ -C ₄ lower alcohol, butylene glycol, propylene glycol, glycerin, hydrous ethanol, hydrolyzed butyleneglycol, Ethyl acetate and a mixed solvent of two or more thereof.

In one embodiment according to the present invention, the C ₁ -C ₄ lower alcohol may be methanol or ethanol.

In one embodiment according to the present invention, in particular, the extract can be obtained by extracting corn and camellia leaves with a mixed solvent of water and ethanol, and the ratio of ethanol and water is about 1: 0.1 to 10 parts by volume, specifically 1: 5 volume ratio, more specifically from 1: 1 to 3 volume ratio, and even more specifically from about 3: 7 volume ratio.

In an embodiment according to the present invention, the water may be distilled water.

In one embodiment according to the present invention, the extract may be an ethanol extract of roots and camellia leaves, respectively.

In one aspect of the present invention, the cosmetic composition may be a soap, a cleansing cream, a cleansing water, a bath, a skin, a lotion, a cream, an essence, a toner, an astringent, an emulsion, a gel, a lipstick, a spray, a shampoo, A body lotion, a body lotion, a body cleanser, a pack, a massage, a face powder, a compact, a foundation, a two-way cake and a makeup base. In one embodiment according to the present invention, the cosmetic composition may be manufactured into a formulation selected from the group consisting of soap, cleansing foam, cleansing cream, cleansing water and bath agent.

In another embodiment according to the present invention, there is provided a process for the preparation of a composition for the manufacture of a medicament for the treatment and / or prophylaxis of cancer, comprising the steps of: (a) treating a herbaceous and camellia plant with water, a C ₁ -C ₄ lower alcohol, butylene glycol, propylene glycol, glycerin, Extraction with a solvent consisting of hexane, ethyl acetate and a mixed solvent of two or more thereof; And (b) mixing the herbal extract and the camphor leaf extract, followed by stirring for 1 to 48 hours.

In one embodiment according to the present invention, in step (b), the gilt and camphor leaf extracts may be blended in a weight ratio range of 10: 3 to 10, specifically 10: 3 to 5, More specifically in a weight ratio range of 10: 4 to 5. However, the lower limit value is more important since the coagulation phenomenon of the extract is not observed when the blend weight ratio of uroguin extract and camphor leaf extract is 10: 3 or more.

In one embodiment according to the present invention, in step (a), the C ₁ -C ₄ lower alcohol may be methanol or ethanol.

In one embodiment according to the present invention, in particular in said step (a), said extract can be obtained by extracting gilt and camellia leaves with a mixed solvent of water and ethanol, the ratio of ethanol to water being about 1: 0.1 to 10 parts by volume, Specifically 1: 0.5 to 5 parts by volume, more specifically 1: 1 to 3 parts by volume, and even more specifically about 3: 7 by volume.

In one embodiment according to the present invention, the water in step (a) may be distilled water.

In one embodiment of the present invention, in step (a), the extract may be ethanol extract of roots and camellia leaves, respectively.

In one aspect of the present invention, the cosmetic composition may be a soap, a cleansing cream, a cleansing water, a bath, a skin, a lotion, a cream, an essence, a toner, an astringent, an emulsion, a gel, a lipstick, a spray, a shampoo, A body lotion, a body lotion, a body cleanser, a pack, a massage, a face powder, a compact, a foundation, a two-way cake and a makeup base. In one embodiment according to the present invention, the cosmetic composition may be manufactured into a formulation selected from the group consisting of soap, cleansing foam, cleansing cream, cleansing water and bath agent.

In addition, the cosmetic composition of the present invention has almost no toxicity because it is a natural product extract or a component separated therefrom as an active ingredient, and is harmless to human body.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

Hereinafter, the present invention will be described in more detail with reference to the following Examples, Experimental Examples and Preparation Examples. It should be understood, however, that the same is by way of illustration and example only and is not intended to limit the scope of the present invention.

Example 1. Preparation of Ulugum extract and Camellia sinensis leaf extract

(1) Root of root of Curcuma longa L. was dried and pulverized and powdered. The ethanol extract was prepared by pulverizing and pulverizing corn in an extraction vessel and immersing it in a 10-fold high-grade alcohol (30% ethanol and 70% distilled water) and heating at 50 ° C for 8 hours. The liquid phase eluted with a filter paper (Whatman paper No 5). The sample solution filtered to volatilize ethanol was concentrated by using a vacuum concentrator at 40 ° C to obtain an acorn extract. On the other hand, in the production of cosmetics using jelly-like curd or powdery curd extract, the curd is highly cohesive and viscous, and because of its strong cohesive nature, it is important to make the curd spread uniformly even if other substances are mixed in the raw material It can be one.

(2) Camellia japonica L.) was collected and dried. Then, a Camellia sinica leaf extract was obtained according to the production process of the extract.

Examples 2 to 5.

The uroguania extract and the camellia leaf extract obtained in Example 1 were compounded in the composition ratios shown in Table 1 below to prepare a cosmetic composition containing urogine and camellia leaf extract. In the case of mixing the extract of Eugyin and Camelliaceae in the solvent, the extract of Eugyung and Camelliaceae were found to be precipitated in the solvent. The precipitated kelp and camphor leaf extracts were subjected to sonication for 1 to 48 hours at 40 to 45 ° C using a spin bar at a speed control of max to allow many active ingredients to flow out into the supernatant.

Mixing ratio of Ulgum and Camellia sinensis leaf extract division Effective concentration Soap 1 unit (100) Comparative Example 1
(100) Example 2
(100) Example 3
(100) Example 4
(100) Example 5
(100) Comparative Example 2
(100) Ulgum extract 1,000 to 500 μg / mL 10g 7.5g 5.0g 4.5 g 5.0g 2.5 g Camellia leaf extract 100 to 50 占 퐂 / mL 2g 2.5 g 2.5 g 3.0 g 5.0g 5.0g Ulgum extract: camellia leaf extract 5: 1 3: 1 2: 1 1.5: 1 1: 1 1: 2

Experimental Example  One. Coherence  Experiment

The degree of agglomeration of the compositions of Examples 2 to 5 and Comparative Examples 1 and 2 was confirmed.

In the case of Comparative Example 1, even when the camphor leaf extract was blended, koi extracts coagulated with each other. Stirring for 1 to 24 hours was required, thereby causing loss of active ingredients and the like.

In the case of Examples 2 to 5, it was found that when the solution was allowed to stand still after sonication, the adhered component of the extract of Ulvae to the inner wall surface of the storage tube was remarkably suppressed. .

On the other hand, in the case of Comparative Example 2, although the uroguanum extract is spread as uniform particles, the content of the camphor leaf extract is larger than that of the uroguanum extract, which may reduce the purpose of the extract. Based on the results of the above experiment, it was concluded that the cosmetic composition containing the extract of Eugyung can be prepared at the blending ratio shown in Table 2 below.

Mixing ratio of Ulgum and Camellia sinensis leaf extract division Effective concentration Soap 1 unit (100) Effective concentration
(500 [mu] g / mL * 100) Effective concentration
(750 占 퐂 / mL * 100) Effective concentration
(1,000 占 퐂 / mL * 100) Ulgum extract (500 ~ 1000 ㎍ / mL) 5g 7.5g 10g Camellia sinensis leaf extract (50-100 ㎍ / mL) 2.5 g 3g 3 ~ 4g

Experimental Example 2. Antimicrobial activity of camphor leaf extract

2-1. Experimental material

The camellia used in this experiment was collected from the wild camellia which is native to Camellia japonica in Cheongbuksan, Jangheung - gun, Jeonnam Province, in February - March, young leaves in April - May, and young leaves in June - August Respectively.

The bacteria used for antimicrobial activity were gram-positive bacteria such as Bacillus subtillis , Streptomyces fradiae and Staphylococcus aureus , Gram-negative bacteria Escherichia coli , Pseudomonas aeruginosa , Enterobacter spp. C1036 and Salmonella typhimurium , respectively.

2-2. Extraction of sample

The cambial green leaves and biotite samples were pulverized to be suitable for extraction. Methanol 10 times the weight of the sample was added and extracted at 40 ° C for 5 to 6 hours. The extracts were then filtered off using a vacuum evaporator and then freeze-dried Dried and then used in the experiment.

2-3. Antimicrobial activity measurement

A single colony of pure strains was plated on a platinum broth and inoculated into 10 ml of broth culture medium. The broth was incubated three times for 18-24 hours at the appropriate temperature for growth of each strain, and then used as an antimicrobial activity test strain. To prepare the plate culture medium for the antimicrobial activity test, 15 ml of each agar medium was sterilized, and 15 ml of the medium was dispensed into a petri dish to coagulate the medium for the base layer. The medium for medium layer to which 0.7% agar was added was added to each 5 ml After sterilization by dispensing in a test tube, 0.1 ml of various test strains (a suspension of bacteria in which the bacterial suspension is made up with sterilized saline at a concentration of 660 nm to 0.3 in sterilized saline) is added aseptically, mixed well and then dispensed on the substrate medium Then, the cells were further solidified to prepare a double medium inoculation medium.

The antimicrobial activity of camellia extract was determined by disc plate method (Piddocket, 1990; Bauer et al., 1996). Namely, the methanol extract of Camellia japonica at the concentration of 1, 5, 10 and 15 mg / ml was filtered with a 0.45 ㎛ membrane filter (Millipore Co., USA), and sterilized on a filter paper disc , Toyo roshi kaisha (8 mm)), and the extraction solvent was aseptically air-dried and completely blown away. The cells were cultured for 24 to 48 hours at the appropriate temperature of the growth of each microorganism, The diameter (mm) of the clear zone was measured to compare the antimicrobial activity. At this time, the solvent and the surfactant used to dissolve each sample were tested.

2-4. Antimicrobial Activity of Methanol Extract on Camellia

Table 3 shows the results of measuring the antimicrobial activity against three Gram-positive bacteria and four Gram-negative bacteria by applying a methanol extract of Camellia japonica to various food spoilage bacteria and food poisoning bacteria by paper disc method (Table 3).

As a result, antimicrobial activity was increased as the concentration of extracts on each part was increased. As the concentration increased, the size of the inhibition zone, which showed antimicrobial activity, was increased. For B. subtilis , S. fradiae , P. aeruginosa, and S. typhimurium in juvenile leaf extracts , Mm inhibitory ring.

Therefore, it was confirmed that the extracts of young leaves in Camellia have wide antibacterial activity against Gram - positive bacteria and Gram - negative bacteria. Among them, S. aureus and E. coli reacted most sensitively and showed inhibition effect of 10 ㎜ when 5 mg / ㎖ of young leaf extract was administered.

In contrast, mature leaf extract showed the antimicrobial activity of the 6 ~ 10㎜ in 15㎎ / ㎖ concentration only for P. aeruginosa, bud extract of P. aeruginosa and Enterobacter spp. C1036 strain at a concentration of 15 mg / ml, the inhibitory effect was 8 mm and 9 mm, respectively.

On the other hand, the antimicrobial effect could not be verified when the concentration of the extract of each part of the camellia used in this experiment was less than 1 mg / ml, and in the case of the extracts of flowers, bark, branches and seeds of Camellia sinensis, Lt; / RTI >

Tanaka et al. (1997) belonging to the same tea plant Camellia (Camellia It has been reported that polyphenolic compounds present in the leaves of sinensis exhibit antimicrobial activity. Phenolic substances act as substrates for the oxidation-reduction reaction and protect the plants themselves by preventing the attack of microorganisms, (Lee et al., 1994). Song et al. (1998) reported that the root of Chenmiraea japonica was extracted with methanol to obtain Agrobacterium rhizogenes , Candida utilis , Saccharomyces cerevisiae and B. subtilis , and reported that the phenolic compounds are involved in the antimicrobial activity. Kim and Han (1997) reported that the antimicrobial compound of sancho is hexadecanoic acid. In addition, Clark et al. (1981) reported that a phenolic substance contained in a plant exhibited an antimicrobial activity. In the present invention, however, the antimicrobial activity varies depending on the content of the phenolic substance contained in the methanol extract of the camellia there was. These results suggest that the antimicrobial activity of young leaf extracts of Camellia japonica differs depending on the content of phenolic substances and the higher the content of phenolic substances, do. (Table 3: Antimicrobial activity of extracts of each part of camellia)

Experimental Example 3. Antioxidant activity of Camellia sinica leaf extract

3-1. Analysis of DPPH radical scavenging ability of extract

For each concentration, 160 μL of the sample was dissolved in methanol and mixed well with 40 μL of a DPPH solution dissolved in methanol at a concentration of 1.5 × 10 ^-4 M. The reaction mixture was allowed to stand at room temperature for 30 minutes and absorbance was measured at 520 nm with a microplate reader spectrophotometer VERSAmax. The free radical scavenging activity was expressed as a percentage and the 50% scavenging concentration (IC ₅₀ expressed in μg / mL or μM) was calculated as compared to the control without the sample. The measured values were expressed as averages of the results obtained by three repeated experiments and compared with the conventional antioxidants Vit C, Vit E and BHT (butylated hydroxytoluene).

- Antioxidant activity of camellia leaves by collection period (December to August)

DPPH is a relatively stable free radical with a deep purple color and is reduced by aromatic compounds and aromatic amines to be discolored. Therefore, DPPH is widely used for searching for antioxidants from various natural materials.

The antioxidative activities of camellia leaf extracts were measured by DPPH method at 100 ㎍ / ㎖ of each period (2005. 12 ~ 2006.8) The DPPH radical scavenging rate increased rapidly to 70.89% in March, and the scavenging rate was 90% or more in the camphor leaf extract collected from May to June, indicating the highest antioxidant activity (Fig. 2). It showed similar antioxidative effect to ascorbic acid (Vit C) and showed antioxidative activity much higher than BHT which is a synthetic antioxidant.

- Antioxidant activity of leaves and leaves (May)

The DPPH radical scavenging activity was about 90% or higher at the concentration of 200 ~ 400 ㎍ / ㎖, but the similar effect was observed at low concentrations And the antioxidative effect was more increased in the leaves than in the leaves. The DPPH radical scavenged more than 87% at the concentration of 25 ㎍ / ㎖ in the leaves, but the scavenging rate was about 25% in the stem leaf.

- Antioxidant activity of camellia by collection time

Camellia leaves were collected monthly from December 2005 to August 2006, and camelliaceae were collected in April and May. The inhibition intensity of each extract was compared with the concentration required to inhibit 50% DPPH free radicals (RC50) (Table 4).

The DPPH radical scavenging activity was measured at 100 ㎍ / ㎖. The DPPH radical scavenging activity of the Camellia japonica leaf extracts in May and June was more than 90% and the RC ₅₀ value was less than 12.5 ㎍ / ㎖ And showed excellent antioxidative effect. Camellia sinensis leaf extracts of 3, 4, 7, and August showed significant antioxidant activity at RC ₅₀ values of less than 60 ㎍ / ㎖, but the leaves collected at 12 ~ 2 months were 3 ~ 4 times higher The RC ₅₀ value was confirmed. (Table 4-Antioxidant Activity)

3-2. Camellia leaves Crude enzyme solution  Catalase ( Catalase ) activation

Catalase (catalase) is an antioxidant enzyme that rapidly treats harmful oxygen in vivo and protects cells. It is a typical enzyme that decomposes and eliminates antioxidant enzyme H ₂ O ₂ . Antioxidant enzyme-rich foods have been thought to reduce the likelihood of developing cancer or heart disease, and antioxidant enzymes have been reported to delay the aging of the body. Vitamin E, beta-carotene, water-soluble vitamin C, selenium (Se), green tea, chlorella, greenish-yellow vegetables, etc. are said to be rich in antioxidant enzymes such as alpha-tocopherol. Among them, vitamin E is known to be the most important fat-soluble antioxidant in humans because it is related to cell membrane. Vitamin C also removes water-soluble peroxidic radicals and regenerates reduced vitamin E.

Catalase activity was measured by the Aebi (1984) method. 10 mM H ₂ O ₂ and a reaction solution were added to 50 mM potassium phosphate (pH 7.0). The change in absorbance at 240 nm for 2 min was observed and the amount of enzyme degrading 1 μM H ₂ O ₂ was set to 1 unit over 1 min.

The catalase activity of the cambium was lower than that of the control. The activity of leaf extracts was highest when compared with the activity of green tea and camellia, and the activity of the leaf was the lowest when compared with that of green leaf (FIG. 4).

The antioxidant activity of green tea has been reported to be high in the past reports. As a result, it is observed that camellia seedlings have higher activity than the green tea activity reported so far and contain substances having high H ₂ O ₂ removal ability.

4. Kool  Bulb extract and essential oil essentila oil ) Of the human body In displeasure  About Deodorizing ability

We analyzed the deodorizing ability of essential oil, floral water and ethanol extracts of Korean ginseng, which has high polyphenol content and strong antioxidant activity and whitening effect.

Vegetable oil is not a single chemical component but a volatile terpene compound. Depending on its functional group, it can be divided into alcohols, aldehydes, ketones, ethers, esters, and organic acids. These functional groups have been reported to react with hydrogen sulfide, ammonia, amines and the like in the odor to change into an odorless basic substance.

The mechanism of elimination of harmful gases, such as the alcohol, aldehyde, and ester groups of Essential oil, caused by the neutralization reaction with ammonia gas, forms salts. The monoterpene molecules in essential oils contain alcohol (-OH) L-linalool, α-terpineol, and geraniol molecules are neutralized with ammonia to form an ammonia salt. As the chain reaction proceeds, ammonia Thereby reducing the gas concentration. Some of the acids and bases produced as a result of the reaction react with the surrounding basicity and turn into salts and water. As described above, when vegetable essential oil coexists with ammonia gas in a certain ratio, the ammonia can be reduced to odorless or almost odorless state.

4-1. Analysis of deodorizing ability of ammonia in the elderly by mixed composition

4-1-1. Experimental Method:

Extract and essential oil composition Preparation: The main ingredient of the composition was the essential oil separated from the bullfruit bulb, the floral water, the ethanol extract, and the golden ethanol extract which had the highest amount of essential oil and polyphenol content of the pine tree. (liquid olive) was used as an emulsifier of essential oil, and ethanol and distilled water were used as a solvent to prepare a total volume of 100 mL. The composition was classified into two types of compositions (A and B) by controlling the concentration after the larvae containing the peony root extract and the peony extract were largely classified (Table 5).

Type of composition and composition ratio main ingredient Composition A Composition B Ulleung oil refinery 150 μl 100 μl Ulgum Floral Water 25 mL 25 mL Ulgum extract 50 mg 10 mg Pine Needle Oil  150 μl 100 μl Golden crude extract  50 mg 10 mg Olive liquid 500 μl 500 μl ethanol 25 mL 25 mL D.W. (purified water) Add to 100 mL Add to 100 mL Total volume 100 mL 100 mL

Measurement of Deodorizing Ability by Gas Detection Method: The deodorizing power of the composition was measured for ammonia which is a representative substance in the air of the elderly smell.

Analytical Procedure: Two 5,000 ml chambers were prepared, and the paper adsorbed on the composition (10 X 20 cm) was dried in a drier capable of holding at 110 ° C until the weight became constant, and then, after cooling in a desiccator, . Ammonia gas was injected into the chamber for the blank and the chamber to which the sample adsorbed the composition was attached so as to be 500 ppm, and then the chamber was immersed in two chambers (initial, 30 minutes, 60 minutes, 90 minutes, and 120 minutes) Was measured by a gas detection tube (Gastec Coporation, Japan / GASTEC No.3M).

      * Calculation of deodorization rate: deodorization rate = (Cb-Cs) / Cb x 100

                        Cb: concentration of background test chamber after a certain time

Cs: Concentration of sample chamber after a certain time elapsed

4-1-2. Experiment result :

The deodorizing power of the two compositions showed that the initial deodorization rate was 69% (Composition A) and 73% (Composition B), respectively. The deodorizing effect of each composition was similar to the initial deodorization rate even at the maximum elapsed time of 120 minutes. It was found that when the composition B showing the best deodorizing power had passed 120 minutes, 85% of the ammonia gas was deodorized. (Table 6 - Deodorizing power of composition A)

(Table 7 - Deodorizing power of Composition B)

4-2. Analysis of nonen-aldehyde deodorizing ability of elderly skin with mixed composition

4-2-1. Experimental Method:

(1) Preparation of extract and essential oil composition: Based on the composition B proved to have the best deodorization ability by the gas detection tube method, the content of the contents and the content ratio of the contents were adjusted to prepare three kinds of compositions (B, B-1 and B-2 ), And the nonenaldehyde released from the elderly skin was set as the deodorizing substance (Table 8).

The kind and mixing ratio of the composition Gradients Composition B Composition B-1 Composition B-2 Ulleung oil refinery 100 μl 40 μl 20 μl Ulgum Floral Water 25 mL 4 mL 2 mL Pine tree oil refinery 100 μl 200 μl 200 μl Ulgum extract 10 mg 5 mg 5 mg Golden crude extract 10 mg 5 mg 5 mg Olive liquid 500 μl 500 μl 500 μl ethanol 25 mL 25 mL 25 mL D.W. (Purified water) Add to 100 mL Add to 100 mL Add to 100 mL Total volume 100 mL 100 mL 100 mL

② direct sensory analysis by law (nonen - aldehyde): the nose of a person albeit much dull compared to animals but can be the most sensitive detection than any other instrument with respect to the low concentration of odors. In addition, since the odor itself is a sensory object, the direct sensory method is applied to directly measure the odor intensity.

* Determination of optimum threshold of odor: When the nonen-aldehyde was diffused in 100 mL of distilled water as the target odor in the range of 5 ~ 10 ㎕, the optimal threshold for odor detection was investigated. As a result, 5 μL of nonenaldehyde Was selected as the optimum value (Table 9).

Gradients Volume Nonen-aldehyde (trans-2-nonenal, 97%, Aldrich) 5 μl D.W. 100 mL Total volume 100 mL

* Sensory Evaluation of Deodorizing Effect on Nonen - aldehyde : 20 persons were used in the experimental group based on Table 10.

burglar condition 0   Odorless One   Almost indistinguishable odor (minimum detection value) 2   Odor that can distinguish what kind of smell (minimum recognition value) 3   Easy to feel odor 4   Strong odor 5   Intolerable aching

4-2-2. Experiment result :

① Evaluation of preference for orientation of the composition

Twenty experimental groups were sprayed once or twice with kimwipes according to the table 10 sensory evaluation table, and then the composition was applied to Kimwipes adsorbed to evaluate the sensation of the olfactory sense Respectively.

As a result of the evaluation, it was found that the composition B was too fragrant to feel a bitter taste (bitter taste), and that the composition B-1 had a slightly lower strength than the fragrance of the composition B, It was an opinion. Composition B-2, which contained low essential oil and floral water content, showed the highest score in the three compositions. In B-2, the fragrant essential oil and floral water content were low, but the fresh pine essential oil flavor was felt and overall feeling was good (Table 11).

Sensory Evaluation by Panel Composition type Average value for overall feeling
(Incense preference) Composition B 4 Composition B-1 6 Composition B-2 9.5

② The composition of the elderly skin Minute jaw ( nonen - aldehyde ) For Deodorant effect

Twenty experimental groups were sprayed twice with nonen-aldehyde on Kim Wipes according to the standard table of Table 10, and the compositions were sprayed twice thereon to evaluate the odor.

The deodorizing powers of the three kinds of composition for the aged skin jade were excellent, and there was no significant difference between the compositions. However, since the fragrance of the composition B and B-1 gave a feeling of rejection to the experimental group in the preference evaluation for the fragrance, the composition B-2 was finally determined as a suitable deodorizing composition.

Composition type Talchwiryeok (indicated by Malordor intensity) Composition B 0.00 (the flavor of the composition felt too strong) Composition B-1 0.25 Composition B-2 0.50

* The lower the value, the better the deodorant power.

③ Older smell of mixed composition ( ammonia , nonen - aldehyde ) Deodorizing effect

Vegetable oil is not a single chemical component but a volatile terpene compound. Depending on its functional group, it can be divided into alcohols, aldehydes, ketones, ethers, esters, and organic acids. These functional groups have been reported to react with hydrogen sulfide, ammonia, amines and the like in the odor to change into an odorless basic substance.

In the present experiment, it was experimentally verified that the deodorant composition B-2 finally determined has significant deodorizing power against non-aldehyde, which is an ammonia odorant in the urine of the elderly and a skin jade. The antioxidant activity and the antioxidant activity of the pine needles and pine needles constituting the composition interact with each other and the components of the ethanol extract of pine needles and gold, which have high antimicrobial activity, interact with the ammonia and nonane, which are the main causes of the smell of the elderly, , And it is thought that it induced the composition change of the neutralization unpleasantness.

5. Inhibitory effect of Ulgum extract on the skin

In the case of the elderly, the cleanliness of the body is low, the metabolism ability is lowered, and fatty acids are secreted through the skin and oxidized to cause discomfort. In addition, the skin supernatants are proliferated more prominently than the normal skin, and the unpleasant odor derived from the skin is increased. Therefore, in this experiment, antimicrobial activity was performed to confirm the preservative and bactericidal effect of extracts and essential oils of three representative skin microorganisms.

5-1. Experimental Method:

1) Strain and medium used : Three strains related to the skin were used for the search for antimicrobial activity. Table 13 shows the strains used in this study and the composition of the medium.

Strains Media Temperature (° C) Propionibacterium acnes TSB 37 Staphylococcus epidermidis TSB 37 Malassezia furfur YM 37

② Antimicrobial activity of the extracts: The antimicrobial activity of the extracts was analyzed using the paper disc method. A single colony of each isolate was isolated and inoculated into 10 ml of the broth culture medium. The broth was cultured for three times at a temperature of 18-24 hours at the appropriate temperature of the strain, and then used as an antimicrobial activity test strain. The preparation of the plate culture medium for the antimicrobial activity test was carried out by sterilizing the growth medium containing 15% agar of each strain and dispensing 15 ml each into the petri dish to coagulate the culture medium for the base layer, density, O, D) to 0.4 (10 ⁶ CFU / mL) and then aseptically added to the medium containing 0.7% agar. The medium was then mixed on the medium for the medium, Medium inoculation medium was prepared. A sterile 8 mm paper disk was placed on a solid medium that was sufficiently hard, and the solution was absorbed by 30 쨉 l of the disk. The plate was incubated at 37 째 C for 24 hours, and a clear zone around the disk was observed.

5-2. Experimental Results: Figures 7 and 8

The antimicrobial activity of the extracts of P. acnes was tested at the concentration of 5 mg / ml, 3 mg / ml and 1 mg / ml of extracts . In S. epidermidis , a clear zone of 2 mm was formed at a concentration of 5 mg / ml, 2 mm at a concentration of 3 mg / ml, and 1 mm at a concentration of 1 mg / ml. In the case of M. furfur , no antimicrobial activity was observed at 1 mg / ml, and antimicrobial activity was observed at 5 mg / ml and 3 mg / ml.

In the case of Ulgum bulb essential oil, it showed strong antimicrobial activity against M. furfur .

Therefore, it was concluded that the addition of ethanol extract to the composition at a concentration of at least 3 mg / ml and at a concentration of 10 μl or more in the case of essential oil would have a considerable effect on the inhibition of the growth of the skin microbes.

Sample Extract concentration 5 mg / ml 3 mg / ml 1 mg / ml Curcuma aromatica 4 mm 3 mm ND

Strains Concentration of essential oil 30 μl 20 μl 10 μl P. ances 3 mm 2 mm 1 mm S. epidermidis 3 mm 1 mm ND M. furfur 4 mm 3 mm 2 mm

6. Antioxidant activity of extract

Active oxygen inhibits the immune function of elderly skin and induces inflammation, causing various skin diseases such as elasticity reduction, wrinkles and spots, and freckles, and ultimately accelerates skin aging. Therefore, in order to prevent aging of the skin, researches on a substance capable of protecting skin cells from active oxygen as well as in vivo have been actively carried out, and the antioxidant activity is widely used as a typical assay method.

Skin antioxidant activity, anti - aging effect, anti inflammation etc.

6-1. DPPH radical scavenging ability of extract

6-1-1. Experimental Method

Antioxidant activity was measured by slightly modifying the Blois method for measuring the radical scavenging effect of the sample using the DPPH method. 100 μl of 1 × 10 ^-4 M DPPH and concentration-specific extracts were mixed and left for 30 min in the dark. The residual radical concentration was measured at 517 nm using an ELISA reader (Bio-RAD, USA). The amount of reducing power of the sample is indicated by the scavenging activity. RC ₅₀ is expressed as the amount of sample (㎍) required for reducing the DPPH concentration to ½, and the BHT (butylated hydroxytoluene) Vit C (ascorbic acid) were compared.

DPPH radical scavenging activity (%) = (Ac-As) / Ac x 100

 Ac: absorbance of control without addition of sample

As: Absorbance of the reaction solution to which the sample is added

6-1-2. result

DPPH is a relatively stable free radical with a dark purple color. It is decolorized by sulfuric amino acid such as cysteine, glutathione, ascorbic acid and BHA, . Free radicals are likely to cause aging by binding to lipids or proteins in the body. In the case of phenolic compounds, they have a strong ability to reduce or offset free radicals and can be used as a measure to inhibit free radical aging in the body.

As a result of analysis of antioxidant activity by RC ₅₀ , which is the amount (μg) of the sample required for decreasing the concentration of DPPH to 1/2, the result was 127 μg / ml (FIG. 9).

Scientific name Korean name RC ₅₀ (占 퐂 / ml) Curcuma aromatica Kool 127 BHT (standard product) - 130.8 Vitamin C (standard product) Vitamin C 9.08

6-2. Antioxidant enzyme activity of extract

Plants are fixed, so their ability to adapt to environmental stress is thought to be higher than other organisms, producing more kinds of antioxidants than other organisms. Cells have enzymatic and non-enzymatic defense systems to protect cellular components and maintain their redox state. In plant non-enzymatic defense systems, there are known natural antioxidants such as ascorbic acid, glutathione, alpha-tocopherol, and carotenoids. Enzymatic defense systems include superoxide dismutase (SOD), catalase (CAT) There are enzymes such as ascorbate peroxidase (APX), glutathione reductase and the like. CAT is an antioxidant enzyme that rapidly treats harmful oxygen in vivo and protects cells. It is a typical enzyme that cleaves H ₂ O ₂ with APX. In plants, APX plays a role as the most important remover in the cytoplasm and chloroplast. They found that ascorbic acid was used as a reducing substrate and GPX (glutathione peroxidases) was found to play an important role in eliminating H ₂ O ₂ and lipid peroxidation.

6-2-1. Experimental Method

- Preparation of enzyme solution

Biological samples were mixed in extraction buffer (50 mM phosphate buffer, pH 7.0; 1% Tritox X-100; 1% PVP-40) at a ratio of 1: 4 and centrifuged at 12,000 rpm for 30 minutes The supernatant was taken and used for antioxidant activity measurement. Protein quantification was performed according to the Bradford (1976) method using BSA as a standard.

- Catalase (CAT) activity measurement

Catalase activity was measured by the Aebi (1984) method. 10 mM H ₂ O ₂ and a reaction solution were added to 50 mM potassium phosphate (pH 7.0). The change in absorbance at 240 nm was observed for 2 minutes, and the amount of enzyme degrading 1 μM of H ₂ O ₂ in 1 minute was set to 1 unit (FIG. 10).

- Determination of the activity of ascorbate peroxidase (APX)

Measurements of APX activity were measured by the method of Nacano and Asada (1981). To 1 ml of the total reaction solution, 50 mM potassium phosphate (pH 7.0), 0.5 mM ascorbate, 0.1 mM H ₂ O ₂ , 0.1 mM EDTA and enzyme solution were added, reacted at 37 ° C for 5 minutes, And the change of absorbance in the minutes was measured (Fig. 11).

- Measurement of SOD (Superoxide dismutase) activity

SOD activity was measured by Beauchamp and Fridovich (1971). A solution containing 50 mM carbonic buffer (pH 10.2), 0.1 mM EDTA, 0.1 mM xanthine, 0.025 mM nitroblue tetrazolium (NBT) and enzyme solution was reacted at 25 ° C for 10 minutes Xanthine oxidase (3.3 * 10 ^-6 mM) was added to measure the reaction. The SOD activity measurement was carried out for 5 minutes at 550 nm for 30 seconds (FIG. 12).

Sample CAT activity
(u / mg proteins) SOD activity
(u / mg proteins) APX activity
(u / mg proteins) Kool 4.6 2.8 3.2

In case of catalase, 4.6 U / mg protein and 2.8 U / mg protein and APX 3.2 U / mg protein were measured by SOD measurement.

Production Example 1. Preparation of Soap

330 mL of water and 175 g of NaOH were mixed and completely dissolved. Then, 5 g and 2.5 g of each of the urophyll and camellia leaf extracts prepared in Example 1 were added to the mixture in small portions for about 30 minutes. The mixture was allowed to dry in a shady, windy environment until dry.

Production Example 2. Preparation of Bath Agent

5 g and 2.5 g of each of the urophyll and camellia leaf extracts prepared in Example 1 were allowed to stand at about 60 ° C, and then a salt solution was prepared to prepare a saturated solution, and water was evaporated. The solution was cooled at room temperature and freeze-dried by rapid freezing. The lyophilized solid was powdered to prepare a bath agent.

Production Example 3. Cleansing Lotion Production

Wool and camellia leaf extract mixture 0.001 wt% Sodium ethylenediaminetetraacetate 0.02 Paraoxybenzoic acid methyl 0.2 glycerin 4 Sodium Hyaluronic Acid 2 Propylene glycol 3 Carbomer 5 Cetearyl alcohol 0.7 Glyceryl stearate 0.5 Sheer butter One P-hydroxybenzoic acid profile 0.1 Macadamia nut oil One Sesquioleic acid 0.5 Glyceryl stearate One Polyoxyethylene sorbitan monooleate 2 Polydensen 5 Mineral oil 20 Dimethicone 5 Stearyl dimethicone 2 Triethanolamine 0.05 Spices Suitable amount Pigment Suitable amount Purified water Balance

The above ingredients were used to prepare the cleansing lotion according to the conventional method in the field of cosmetic production.

Production Example 4. Skin Production

Wool and camellia leaf extract mixture 0.001 wt% Paraoxybenzoic acid methyl 0.2 Sodium ethylenediaminetetraacetate 0.02 1,3-butylene glycol 0.02 Allantoin 3 Sodium hyaluronic acid seed 5 Carbomer 0.1 Cetostearyl alcohol 0.7 P-hydroxybenzoic acid profile 0.1 Sorbitan olivate 1.5 Soy lecithin 0.2 Dimethicone 0.2 Cetyl octanoate 0.2 Sheer butter 0.2 Sodium polyacrylate 3 Triethanolamine 0.1 Imidazolidinyl urea 0.3 Spices Suitable amount Pigment Suitable amount Purified water Balance

These ingredients were used to make the skin according to the conventional method in the field of cosmetics for making a skin.

Preparation Example 5. Preparation of serum

Wool and camellia leaf extract mixture 0.001 wt% Jojoba 5 Black Sesame 2 Sweet almond 3 This malting wax One Vitamin E One glycerin 2 Hyaluronic acid One Marine Elastin One

The above ingredients were used to prepare according to the conventional method in the field of cosmetic production for the production of serum.

Production Example 6. Production of Lotion

Wool and camellia leaf extract mixture 0.001 wt% Paraoxybenzoic acid methyl 0.2 Sodium ethylenediaminetetraacetate 0.02 1,3-butylene glycol 0.02 Allantoin 3 Sodium hyaluronic acid seed 5 Carbomer 0.1 Cetostearyl alcohol 0.7 P-hydroxybenzoic acid profile 0.1 Sorbitan olivate 1.5 Soy lecithin 0.2 Dimethicone 0.2 Cetyl octanoate 0.2 Sheer butter 0.2 Sodium polyacrylate 3 Triethanolamine 0.1 Imidazolidinyl urea 0.3 Spices Suitable amount Pigment Suitable amount Purified water Balance

The above ingredients were used to prepare according to conventional methods in the field of cosmetics for the manufacture of lotions.

Production Example 7. Preparation of Essence

Wool and camellia leaf extract mixture 0.001 wt% Allantoin 0.05 Sodium ethylenediaminetetraacetate 0.02 Triethanolamine 0.2 Sodium Hyaluronic Acid 7 Imidazolidinyl urea 0.15 Sodium polyacrylate 0.4 Carbomer 0.2 ethanol 3 Polyoxyethylene sorbitan monostearate 0.2 Paraoxybenzoic acid methyl 0.2 Spices Suitable amount Pigment Suitable amount Purified water Balance

The above ingredients were used to prepare according to a conventional method in the field of cosmetic preparation for essence production.

Preparation Example 8. Preparation of Cream

Wool and camellia leaf extract mixture 0.001 wt% Sodium ethylenediaminetetraacetate 0.02 Allantoin 0.1 glycerin 5 Paraoxybenzoic acid methyl 0.2 Sodium Hyaluronic Acid 6 Carbomer 0.1 Cetostearyl alcohol 1.7 Polydensen 2 Squalane 2 P-hydroxybenzoic acid profile 0.1 Butylene glycol dicaprylate 3 Cetyl octanoate 5 Microcrystalline lead 0.1 Triethylpentanediol 0.1 Sheer butter 0.2 Sorbitan olivate 0.3 Cyclomethicone 0.3 Stearyl dimethicone 0.5 Imidazolidinyl urea 0.15 Spices Suitable amount Pigment Suitable amount Purified water Balance

These ingredients were used to prepare the cream according to the conventional method in the field of cosmetic production.

Production Example 9. Pack Production

Wool and camellia leaf extract mixture 0.001 wt% Sodium ethylenediaminetetraacetate 0.02 Betaine 3 Glyceryl polymethacrylate 2 Allantoin 0.1 Sodium Hyaluronic Acid 2 glycerin 3 Dipropylene glycol 5 Paraoxybenzoic acid methyl 0.2 Polyvinyl alcohol 10 Polyoxyethylene sorbitan monooleate 0.9 Sesquioleic acid 0.3 Jojoba ester 2 Cetearyl alcohol 1.5 Petrolatum 0.5 Spices Suitable amount Pigment Suitable amount Purified water Balance

The ingredients were prepared according to the conventional method in the field of cosmetic production for pack manufacture.

Manufacturing Example 10. Massage Cream Production

Wool and camellia leaf extract mixture 0.001 wt% glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.5 Squalane 2.5 Wax 2.1 Tocopheryl acetate 0.1 Cava Paul 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount Purified water Balance

The ingredients were used to make the usual methods in the field of cosmetic manufacturing for the production of massage cream.

Production Example 11. Manufacture of make-up base

Wool and camellia leaf extract mixture 0.001 wt% Coated silica One Silica 10 Titanium dioxide 8 Zinc oxide 3 Pigment One Plate powder Balance

These ingredients were used to make the makeup base according to the conventional method in the field of cosmetic production.

Production Example 12. Production of Powder Fact

Wool and camellia leaf extract mixture 0.001 wt% Mica 15 Titanium dioxide 7 Silicone oil 3 Ester oil 3 Pigment Suitable amount Spices Suitable amount Talc Balance

The powder facts were prepared according to a conventional method in the field of cosmetic production for the powder fact using the above-mentioned components.

Production Example 13. Two-way cake production

Wool and camellia leaf extract mixture 0.001 wt% Mica 15 Titanium dioxide 12 Silicone oil 3 Ester oil 5 Pigment Suitable amount Spices Suitable amount Talc Balance

The ingredients were used to make the usual methods in the field of cosmetics for the manufacture of two-way cakes.

Claims (10)

A cosmetic composition for antioxidant, antibacterial, and deodorant comprising, as an active ingredient, a mixture of a lower alcohol extract of C ₁ ~C _{4 and} a lower alcohol extract of camellia leaves C ₁ ~C ₄ in a weight ratio of 10: 3 ~ 10.
delete delete delete The method according to claim 1,
The cosmetic composition for antioxidant, antibacterial, and deodorant according to claim ₁ , wherein the lower alcohol extract of Cul-C ₁ to C ₄ is ethanol extract of roots of roots and the lower alcohol extract of camphor leaves C ₁ to C ₄ is an ethanol extract of camellia leaves .
The method according to claim 1,
The cosmetic composition may be in the form of a lotion or a lotion such as a soap, a cleansing foam, a cleansing cream, a cleansing water, a bath agent, a skin lotion, a cream, an essence, a toner, an astringent, an emulsion, a gel, a lipstick, a spray, a shampoo, a rinse, Wherein the cosmetic composition is produced into a formulation selected from the group consisting of a powder, a powder, a powder, a compact, a foundation, a two-way cake and a makeup base.
The method according to claim 1,
Wherein the cosmetic composition is produced into a formulation selected from the group consisting of soap, cleansing foam, cleansing cream, cleansing water and bath agent.
The method according to claim 1,
The cosmetic composition for antioxidation, antibacterial and deodorization according to claim 1, wherein the cosmetic composition is produced by soap.
(a) extracting each of the wool and camellia leaves with a C ₁ -C ₄ lower alcohol; And
including the step of stirring and then mixed in a weight ratio range of from 3 to 10 1 to 48 hours: (b) the turmeric C ₁ ~ C ₄ lower alcohol extract and Camellia leaves C ₁ ~ C ₄ of the lower alcohol extract 10 , Antioxidant, antibacterial and deodorant. delete

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