KR102596573B1 - Cosmetic composition containing Cyperus sanguinolentus extract for skin whitening - Google Patents

Abstract

The present invention relates to a composition containing an extract from the head of Bangdong sani, especially the leaves and stems, which can be easily used as a multifunctional cosmetic composition that improves skin condition by inhibiting the activity of tyrosinase.

Description

Cosmetic composition for whitening containing Cyperus sanguinolentus extract for skin whitening}

The present invention relates to a cosmetic composition for skin whitening containing Cyperus sanguinolentus extract.

Hyperpigmentation of the skin is caused by various factors such as hormonal abnormalities in the body following an inflammatory reaction of the skin, genetic diseases, and ultraviolet irradiation. The main factor is abnormal melanin pigment synthesis and distribution. Melanin is produced by converting tyrosine into dopaquinone within melanocytes by the action of tyrosinase, and then dopaquinone is converted into melanin by the action of enzymes and spontaneous oxidation reactions.

There are the following methods to inhibit melanin production. The first method is to block ultraviolet rays to remove the main cause of melanin production. A whitening reaction can be induced by producing cosmetics containing light scattering agents or light blockers. . The second method is to suppress melanin production by inhibiting the synthesis of core carbohydrates necessary for tyrosinase to be active, and can use compounds such as glucosamine. The third method is a method of interfering with cell division of melanin-producing cells using a substance that has specific toxicity to melanocytes, and a compound such as hydroquinone can be used. The fourth method is to interfere with the function of tyrosinase, an enzyme involved in melanin production, and can use compounds such as kojic acid or arbutin. Another method is to reduce the produced melanin. When using compounds such as hydroquinone, kojic acid, or arbutin in the above method, compounds derived from natural products may be used, but synthetic compounds may also be used. Representative synthetic compounds used include, in addition to hydroquinone, kojic acid or arbutin mentioned above, 4-n-butylresorcinol, quercetin, kaempferol, etc. Although the above synthetic compounds have a good whitening effect, their use is limited due to product safety issues due to skin irritation. In particular, synthetic 4-n-butylresorcinol has poor stability during production, easily turns brown, and may cause skin irritation when used in high concentrations. Therefore, in order to solve these shortcomings, a method of liposomalizing 4-n-butylresorcinol using gamma-linolenic acid has been disclosed (Korean Patent No. 10-0751883), but it was impossible to completely eliminate irritation. .

Cyperus sanguinolentus is an annual plant distributed throughout the country. The leaves grow at the bottom and are 2-3 mm wide. The bottom is tube-like and shorter than the peduncle. The leaf sheath is green, but the bottom is brown. Flowers bloom from July to October, have 2-3 bracts, look like leaves, and are longer than the inflorescence and spread out laterally. The inflorescence is capitated with 3-10 spikelets with short stems hanging in an umbel shape and has a diameter of 1.5-3.5cm. The stem sometimes splits into 2 or 3 branches. Spikelets are flat, long oval or lanceolate, 1-2 cm long and 2.5-3 mm wide, with pointed ends and 15-30 flowers. The glumes are broadly ovate, 2-2.5mm long, have a blunt end, and have grooves on both sides. The style is about three times longer than the achene and is deeply split into two. The achene is about 1 mm long, has a wide obovate shape, is flat on the left and right, has fine spots that can be seen through a magnifying glass, and is dark brown in color. It grows in groups, is triangular, is 15 to 40 cm high, and stands diagonally at the bottom. The physiological efficacy has not been studied much yet.

Accordingly, while studying various physiological activities of the Bangdong sani head extract, the present inventors confirmed that the extract had an excellent skin whitening effect and completed the present invention.

Republic of Korea Registered Patent No. 10-1908221 (Title of invention: Anti-obesity composition containing extract of Geumdong snail, Applicant: Dongeui University Industry-Academic Cooperation Foundation) Republic of Korea Patent Publication No. 10-2015-0026579 (Title of the invention: Pharmaceutical composition and health functional food for the prevention and treatment of allergic or non-allergic skin diseases containing herbal extracts as active ingredients, Applicant: YD Life Science Co., Ltd. ) Republic of Korea Patent No. 10-0751883 (Title of the invention: Whitening cosmetic composition containing gamma-linolenic acid-4-n-butylresorcinol water-soluble nanoliposomes, Applicant: Wellskin Co., Ltd.)

The purpose of the present invention is to provide a cosmetic composition for skin whitening containing Cyperus sanguinolentus extract.

The present invention relates to a cosmetic composition for skin whitening containing Cyperus sanguinolentus extract.

The extract has tyrosinase inhibitory activity.

The extract is characterized in that it is extracted using water, C1 to C4 alcohol, or a mixed solution thereof as a solvent. The C1 to C4 alcohol may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, and isobutanol.

The cosmetic composition includes skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, pack, soap. , it may have any one formulation selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

Additionally, the present invention may relate to a health functional food for skin whitening containing Bangdong sani head extract.

Hereinafter, the present invention will be described in detail.

The Bangdong sani head extract may be extracted from the leaves, stems and branches, roots, flowers, fruits, or mixtures thereof of the collected plant using an extraction solvent. Preferably, leaves and stems can be mixed at a weight ratio of 1:0.5 to 1:2.

The Bangdong sani head extract can be prepared using water, C1 to C4 alcohol, or a mixed solution thereof as a solvent. The water can be any water without impurities, such as distilled water or ionized water (DI water). The C1 to C4 alcohol may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, and isobutanol. At this time, it is better to use a C1~C4 alcohol extract or an aqueous solution extract of C1~C4 alcohol rather than a water extract, and the aqueous solution extract of C1~C4 alcohol has the best skin whitening activity. A suitable aqueous solution of C1 to C4 alcohol is a 50 to 90% (v/v) ethanol aqueous solution, and the most suitable is a 60 to 70% (v/v) ethanol aqueous solution.

When preparing the Bangdong Sani head extract, it can be extracted using a solvent with a weight of 5 to 40 times, preferably 10 to 20 times, the dry weight of the Bangdong Sani head. If the solvent is too less than the above range, when preparing the extract by applying heat, extraction may be difficult due to evaporation of the solvent, and if the solvent is too much, the extraction time may be too long.

Additionally, the extraction process can be performed at 10 to 40°C with stirring for 24 to 48 hours, and extraction can be repeated 1 to 4 times to obtain a liquid extract.

The liquid extract can be filtered of dry matter, etc. using sterile gauze or filter paper. The filtrate through this filtration process can be concentrated under reduced pressure at 10-40°C using a rotary vacuum concentrator, and the extract can be obtained after removing the remaining solvent by freeze-drying.

The Bangdongsani head extract may be included in the cosmetic composition in an amount of 0.01 to 20% by weight. If the Bangdongsani head extract is included in the cosmetic in an amount of less than 0.01% by weight, the skin whitening effect may be minimal, and if it exceeds 20% by weight, formulation may become difficult. In addition, if it exceeds 10% by weight, the degree of improvement in skin whitening functionality may become insignificant, so it may be preferable that the cosmetic composition contains 0.01 to 10% by weight of the Bangdong Sanidae Gari extract. At this time, the weight of the Bangdong snake head extract is based on the dried Bangdong snake head extract made into powder form.

Additionally, the present invention can provide a cosmetic formulation containing the cosmetic composition as a raw material or active ingredient. The above formulations include skin ointment, essence, whitening cream, lotion, emulsion, pack, general lotion, skin milk, skin, cream, serum, beauty soap, softening lotion, medicated lotion, body cleanser, cleansing oil, cleansing cream, and cleansing tissue. and cleansing water.

The cosmetic formulation of the present invention includes animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, lactose, silica, aluminum hydroxide, as carrier ingredients. Calcium silicate, polyamide powder, chlorofluorohydrocarbon, propane/butane or dimethyl ether, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, Suspending agents such as glycerol aliphatic esters, fatty acid esters of polyethylene glycol or sorbitan, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, Agar or tracant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty acid glyceride. , fatty acid diethanolamide, lanolin derivatives, or ethoxylated glycerol fatty acid esters can be used.

In addition, the present invention provides a health functional food for skin whitening containing Bangdong sani head extract.

The Bangdong sani head extract can be added in an amount of 0.01 to 100% by weight to the health functional food of the present invention. The health functional food of the present invention includes the form of tablets, capsules, pills, or liquid, and foods to which the extract of the present invention can be added include, for example, various drinks, meat, sausages, bread, candy, These include snacks, noodles, ice cream, dairy products, soups, electrolyte beverages, beverages, alcoholic beverages, gum, tea, and vitamin complexes.

The present invention relates to a composition containing an extract from the head of Bangdong sani, especially the leaf and stem extracts, which have excellent tyrosinase activity and can be easily used as a multifunctional cosmetic composition that improves skin condition. .

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to ensure that the content introduced here is thorough and complete, and to sufficiently convey the spirit of the present invention to those skilled in the art.

Preparation Examples 1 to 3. Preparation of extracts of Snail head, White Snail or Hyangbuja

To prepare the Bangdong sani head extract of the present invention, the leaves and stems of the Bangdong sani head were mixed in equal amounts, washed with tertiary distilled water, and finely ground using a blade. Extraction was performed by adding 10 times the weight of 70% (v/v) ethanol aqueous solution to an equal amount of crushed leaves and stems of Bangdong sani head and stirring at 25°C for 24 hours. After first filtering using sterile gauze, the filtrate was filtered again using filter paper (Whatman, No. 2: Whatman Ltd., Maidstone, Kent, UK) to filter out the ground samples, etc., and then using a rotary vacuum concentrator. After concentration under reduced pressure, the remaining solvent was removed with a freeze dryer to obtain a Bangdong sani head extract. In addition, as shown in Table 1 below, extracts were prepared for Cyperus pacificus or Cyperus rotundus using samples from the same area.

condition sample Manufacturing Example 1 Bangdongsani Head Production example 2 White-faced snail Production example 3 Hyangbuja

Example 1. Confirmation of tyrosinase inhibitory activity of Bangdongsani head extract

To measure tyrosinase inhibitory activity, a widely used mushroom tyrosinase was used. Prepare a solution of 1 mM L-tyrosine and 0.5 M phosphate buffer (pH 6.5) in a 96-well microplate (SPL Life Sciences Co., Ltd., Pocheon, Korea). The extract samples whose activity was to be measured were reacted with 170 μl of the prepared solution and 20 μl of tyrosinase (300 U) at 25°C for 30 minutes. After reaction, it was measured at 450 nm using a microplate reader (Berthold, Bad Wildbad, Germany). 10 μl of DMSO was added and used as a control. Blank was measured by adding 20 μl of 0.1 M phosphate buffer (pH 6.5) instead of tyrosinase.

The enzyme activity inhibition rate (%) was calculated using the formula below.

Tyrosinase activity inhibition rate (%) = {(A-B)/A} ×100

A: O.D at 450 nm without sample

B: O.D at 450 nm with sample

division Relative absorbance compared to control group
(Relative tyrosinase activity, Abs) Control (DMSO) 1.00 Manufacturing Example 1 0.499±0.02 Production example 2 1.08±0.08 Production example 3 0.97±0.12

As a result, as shown in Table 2, it can be seen that the inhibitory effect of tyrosinase was very excellent in the group treated with the Bangdong snail head extract. However, extracts from the same genus of plants such as P. lily and Hyangbu-ja showed little efficacy.

<Formulation example 1. Cosmetic production>

Formulation Example 1-1. Manufacturing of Skins

Preparation Example 1 of the present invention contains 5.0% by weight of Bangdongsani head extract, 5.2% by weight of propylene glycol, 1.5% by weight of oleyl alcohol, 3.2% by weight of ethanol, 3.2% by weight of polysorbate 20, 2.0% by weight of benzophenone-9, carboxyl The skin was prepared using a conventional method with the following contents: 1.0% by weight of vinyl polymer, 3.5% by weight of glycerin, a trace amount of fragrance, a trace amount of preservative, and the remaining amount of purified water.

Formulation Example 1-2. Manufacture of lotions

5.0% by weight of Bangdongsani head extract of Preparation Example 1 of the present invention, 1.0% by weight of cetostearyl alcohol, 0.8% by weight of glyceryl monostearate, 0.3% by weight of sorbitan monostearate, 1.0% by weight of polysorbate 60, mineral oil Typical contents include 5.0% by weight, cyclomethicone 3.0% by weight, dimethicone 0.5% by weight, allantoin 0.1% by weight, glycerin 5.0% by weight, alcohol 2% by weight, propylene glycol 3.0% by weight, a trace amount of flavor, a trace amount of preservative, and the remaining amount of purified water. The lotion was prepared using the phosphorus method.

Formulation Example 1-3. Preparation of Essence

5.0% by weight of Bangdongsani head extract of Preparation Example 1 of the present invention, 4.0% by weight of propylene glycol, 3.0% by weight of glycerin, 0.5% by weight of allantoin, 0.01% by weight of EDTA-2Na, 5.0% by weight of ethanol, 1.5% by weight of triethanolamine, 2.0% by weight of squalane. Essence using a conventional method with the following contents by weight, 2.5% by weight of beeswax, 60% by weight of polysorbate, 1.0% by weight of carboxylic vinyl polymer, 2.5% by weight of sorbitan sesquioleate, trace amount of fragrance, trace amount of preservative, and remaining amount of purified water. was manufactured.

Formulation Example 1-4. Preparation of Cream

Preparation Example 1 of the present invention: 10.0% of Bangdong sani head extract, 0.7% of polyoxyethylene sorbitan monostearate, 0.5% of sorbitan sesquioleate, 0.6% of cetyl alcohol, 0.75% of stearic acid, 0.6% of glyceryl monostearate, liquid. A cream was prepared using a conventional method with the following contents: 15.0% paraffin, 10.0% carboxyvinyl polymer, 0.2% triethanolamine, a trace amount of flavor, a trace amount of preservative, and the remaining amount of purified water.

<Formulation example 2. Food production>

Formulation Example 2-1. Manufacturing of cooking seasoning

A cooking seasoning for health promotion was prepared by adding 1% by weight of the Bangdong sani head extract of Preparation Example 1 of the present invention to the cooking seasoning.

Formulation Example 2-2. Manufacturing of flour foods

The extract from Preparation Example 1 of the present invention was added at 0.1% by weight to flour, and this mixture was used to prepare bread, cakes, cookies, crackers, and noodles to prepare food for health promotion.

Formulation Example 2-3. Preparation of soups and gravies

Health-promoting soup and gravy were prepared by adding 0.1% by weight of the Bangdong sani head extract of Preparation Example 1 of the present invention to soup and gravy.

Formulation Example 2-4. Manufacturing of dairy products

The Bangdongsani head extract of Preparation Example 1 of the present invention was added at 0.1% by weight to milk, and various dairy products such as butter and ice cream were manufactured using the milk.

Formulation Example 2-5. Manufacturing of vegetable juice

Vegetable juice for health promotion was prepared by adding 0.5 g of the Bangdong sani head extract of Preparation Example 1 of the present invention to 1,000 ml of tomato juice or carrot juice.

Formulation Example 2-6. Manufacturing of fruit juice

Health-promoting fruit juice was prepared by adding 0.1 g of the Bangdong sani head extract of Preparation Example 1 of the present invention to 1,000 ml of apple juice or grape juice.

Claims (6)

It contains an extract obtained by stirring a mixture of leaves and stems of Cyperus sanguinolentus at 10 to 40°C for 24 to 48 hours using a 50 to 90% (v/v) ethanol aqueous solution as a solvent to inhibit tyrosinase. An effective cosmetic composition for skin whitening, wherein the tyrosinase activity of the extract-treated group is suppressed by 0.499 ± 0.020 fold compared to the untreated group. delete delete It contains an extract obtained by stirring a mixture of leaves and stems of Cyperus sanguinolentus at 10 to 40°C for 24 to 48 hours using a 50 to 90% (v/v) ethanol aqueous solution as a solvent to inhibit tyrosinase. An effective cosmetic composition for skin whitening, wherein the tyrosinase activity of the extract-treated group is suppressed by 0.499 ± 0.020 fold compared to the untreated group. delete delete