KR20110049575A - Composition comprising the essential oil extract of laminaria japonica showing anti-oxidant activity - Google Patents

Abstract

PURPOSE: A composition containing Laminaria japonica extract with an antioxidation activity is provided to remove free radical and to prevent oxidative stress-associated diseases. CONSTITUTION: A pharmaceutical composition for preventing and treating oxidative stress-associated diseases contains Laminaria japonica extract with an antioxidation activity as an active ingredient. The Laminaria japonica extract is isolated using water, ethanol, or mixture solvent. A health food for preventing and treating oxidative stress-associated diseases contains the Laminaria japonica extract as an active ingredient. The health food is manufactured in the form of powder, granule, tablet, capsule, or beverage.

Description

Composition comprising the essential oil extract of Laminaria japonica showing anti-oxidant activity}

The present invention relates to a composition for the prevention and treatment of a composition containing kelp extract having antioxidant activity as an active ingredient.

[Reference 1] Tiidus PM., Et al., Sported Med, 20 , pp. 12-13, 1995

Britton, C., et al., Hydroperoxide metablism in mammalian organ, Physio. Rev., 59 , pp. 527-605, 1979

Ishinaga M, et al., Daily intake of fatty acids, sterols, and phospholipids by Japanese women and serum cholesterol, J. Nutr. Sci. Vitaminol, 40 , pp. 557-567, 1994

Kumar CT., Et al., Mol. Cell Biochem., 111 , pp. 109-115, 1992

[Reference 5] Korea National Statistical Office: The cause of death Statistics 2003

Document 6: Annual Report of on the Cause of Death Statistics. Korea National Statistical Office, 2004

7 Bray, TM Nutrition. 16 , pp. 578-581, 2000

8 Fridovich et al., Ann. Rev. Biochem., 44 , pp. 147-159, 1975

9, Tauberet al., Blood, 53 , pp. 666-676, 1979.

10. Borg, DC and Schaich, KM., Handbook of Free Radicals and Antioxidant in Biomedicine, Vol. I, pp. 63-80, CRC press, 1989

[11] Davies, KJA and Goldberg, AL., J. Biol. Chem., 262 , pp. 8220-8226, 1987

12 Morehouse, LA and Thomas, CE., J. Free Radicals Biol. Med., 1 , p. 8, 1985

[13] Harman, D., Free Radicals in Biology, Academic Press, New York, pp. 255-275, 1982

Document 14 Branen., AL, J. Am. Oil. Chem. Soc., 55 , pp. 123-126, 1975

15 Ito N., et al., J. Natl. Cancer Inst., 70 , pp. 343-349, 1983

16. Lim DK., Et al., Korean J. Food Sci. Technol., 28 , pp.83-89, 1996

17. Kim HK., Et al., Korean J. Food Sci. Technol., 27 , pp. 80-85, 1995

18. Cha JY., J. Korean Soc, Food Nutr., 28 , pp. 1131-1136, 1999

[19] Kim MH., Et al., Korean J. Food Sci. Technol., 31 , pp. 273-279, 1999

Blois, MS: Antioxidant determination by the use of a stable free radical, Nature, 26 , pp. 1199-1200, 1958

Bios, MS, Antioxide determination by the use of a stable free radical, Nature. 4617, pp.1198, 1958

[Reference 22] Shin YS. et al., Antioxidant and antimicrobial Effects of extract with water and ethanol of oriental melon (Cucumismelo L. varmakuwa Makino), J Korean Soc Appl Biol Chem., 51 (3) , pp.194-199, 2008

The present invention provides a composition containing kelp extract as an active ingredient, specifically, a pharmaceutical composition, a dietary supplement, a food additive, or an antioxidant for preventing and treating oxidative stress.

Antioxidation refers to the prevention of various oxidation reactions in the body. Lipids present as biofilms or lipoproteins are attacked by free radicals in the body to form various kinds of peroxides. Decomposition products are highly reactive and convert and increase the structure and function of surrounding biomolecules, leading to aging and various chronic diseases. There are several antioxidant defenses in the body that can neutralize these free radicals and protect the body. Human disease and aging occur during metabolism in the body: O _2- (superoxide), nitric oxide (NO), nitrogen dioxide (NO ₂ ), hydroxy (OH), proxy (ROO), ro (alkoxyl), HO ₂ ( This is due to oxidation reactions such as hydroperoxyl radicals, and in vivo, as a means of defending the body from these free radicals, which are harmful radicals, superoxide dismutase (SOD), an antioxidant enzyme found in liver and red blood cells, antioxidants, such as super oxide radical by reducing (superoxide radical) sikimyeo converted to H ₂ O ₂ in this case the generated H ₂ O ₂ and the like catalase (CAT;; catalase), glutathione peroxidase (glutathione-peroxidase GSH-px) Antioxidants such as enzymes, vitamin E, and glutathione are present to protect the living body from oxidative damage by removing free radicals (Tiidus PM., Et al., Sported Med, 20 , pp. 12-13, 1995; Britton, C., et al., Hydroperoxide metablism in mammalian organ, Physio.R ev., 59 , pp. 527-605, 1979; Ishinaga M, et al., daily intake of fatty acids, sterols, and phospholipids by Japanese women and serum cholesterol, J. Nutr. Sci. Vitaminol, 40 , pp. 557 -567, 1994).

However, when these antioxidant defenses are too low to control the production of free radicals, oxidative stress and damage of tissues are promoted. Excess free radicals and lipid peroxides produced in the body are injured by oxidative stress such as protein oxidation and DNA damage. Increase (Kumar CT., Et al., Mol. Cell Biochem., 111 , pp. 109-115, 1992).

Currently, the main causes of death in Korea in 2003 were cancer, cerebrovascular disease, cardiovascular disease, and diabetes mellitus (Korea National Statistical Office: The cause of death Statistics 2003; Annual Report of on the Cause of Death Statistics. National Statistical Office, 2004). Reactive oxygen species (ROS) may be a major factor causing chronic degenerative diseases such as cardiovascular disease, diabetes mellitus, cancer, degenerative neuropathy and aging, and antioxidants are known to help prevent chronic degenerative diseases. (Bray, TM Nutrition. 16 , pp. 578-581, 2000).

Since all aerobic organisms inevitably carry harmful free radical species during metabolism, all organisms must have a mechanism to remove these free radical species in order to survive. Active oxygen species can be classified into two types, compounds that form oxygen radical species such as hydrogen peroxide and oxygen radical species. As already known, the hydrogen peroxide is catalase (catalase) or the case of peroxidase (peroxidase) oxygen, but the conversion of water, oxygen radical species are removed by the mechanism, O ₂ ^- other oxygen radicals other than the mechanism of removing (superoxide anion) Species removal mechanisms are not yet clear.

Hydroxy radicals, a type of oxygen radical species, are formed by the reaction of H ₂ O ₂ and O ₂ in living organisms and are known to exist in almost all biomolecules (Fridovich et al., Ann. Rev. Biochem., 44 , pp. 147-159, 1975; Tauberet al., Blood, 53 , pp. 666-676, 1979). Therefore, it is obvious that defense against highly reactive hydroxy radicals is required in order to maintain homeostasis in vivo.

In this respect, organic materials such as ascorbic acid and uric acid are known to remove hydroxy radicals by chemical reaction, but this is not fundamentally to remove hydroxy radicals but to convert them into less reactive radicals, as well as oxidizing agents in vivo. It has been reported to react with transition metals, such as iron, rather to promote the production of reactive oxygen radical species (Borg, DC and Schaich, KM., Handbook of Free Radicals and Antioxidant in Biomedicine, Vol. I, pp. 63-80, CRC press, 1989). Physiological processes radicals generated during ^{_{(O 2 -, H 2 O}} 2, OH and O ₂₎ is a free radical reaction in which a very strong reactivity caused by these represents the destruction effect of major macromolecules, including lipids (Davies , KJA and Goldberg, AL., J. Biol. Chem., 262 , pp. 8220-8226, 1987). Their production is increased by a number of factors that cause abnormal metabolic processes, photosensitization, drug metabolism, and other cellular metabolism, and therefore, living organisms are always exposed to the harmful effects of free radical reactions caused by them. (Morehouse, LA and Thomas, CE., J. Free Radicals Biol. Med., 1 , p. 8, 1985). In particular, if cells continue to accumulate harmful effects due to their harmful radicals as they age, they cause aging of the cells as well as diseases related to various adult diseases such as carcinogenesis, arteriosclerosis, heart disease, and skin aging. It has been suggested as a cause of human disease occurrence and aging (Harman, D., Free Radicals in Biology, Academic Press, New York, pp.255-275, 1982).

Therefore, the candidate is expected to be useful for treating diseases such as lipid metabolism, immune disease and cancer by inhibiting free radical reaction in vivo by reactive oxygen metabolites or removing harmful free radicals accumulated in the human body, thereby removing lipids. Research has continued to find a substance.

Therefore, many synthetic or natural antioxidants have been developed for the reduction of oxidative stress, but synthetic antioxidants have a problem in safety (Branen., AL, J. Am. Oil. Chem. Soc., 55 , pp. 123-126, 1975; Ito N., et al., J. Natl. Cancer Inst., 70 , pp. 343-349, 1983).

Recently, carotenoids, flavonoids, and polyphenols, which are present in various herbal medicines and edible plant extracts, along with vitamins A, C, and E, are used to develop safer and more natural antioxidants. (Lim DK., Et al., Korean J. Food Sci. Technol., 28 , pp. 83-89, 1996; Kim HK., Et al., Korean J. Food Sci. Technol., 27 , pp. 80-85, 1995; Cha JY., J. Korean Soc, Food Nutr., 28 , pp. 1131-1136, 1999; Kim MH., et al., Korean J. Food Sci.Technol., 31 , pp. 273-279, 1999).

Laminaria is a genus of brown seaweed kelp, a genus of seaweed, which is distributed along the coast of Hanbok, Korea. It is known to grow in the north east coast, Hamgyongnam-do north of Wonsan, and North Hamgyong in Korea. . It is also distributed in the Pacific coasts of Hokkaido and Tohoku, Japan, Kamchatka Peninsula and Sakhalin Island. Kelp plants grow over 20 species on the Pacific coast. Major species include Laminaria japonica, Laminaria ochotensis, and Laminaria religiosa. It has been edible in Korea, Japan and China since ancient times. In addition to various pigments such as carotene, xanthophylls, and chlorophyll, kelp contains a lot of carbohydrates, such as manit and laminarin, which are made by carbon assimilation, and alginic acid, which is a component of cell walls. This contains. The ingredients vary depending on the type, but are generally 16% moisture, 7% protein, 1.5% fat, 49% carbohydrate, and 26.5% inorganic salts. 20% of the carbohydrates are fiber and the rest are polysaccharides such as alginic acid and laminarin. In particular, since a lot of inorganic salts such as iodine, potassium, calcium, etc., eating a little bit of kelp is good for the supply of inorganic salts. The amino acid called laminin in Dashima has the effect of lowering blood pressure.

Therefore, the present inventors conducted a continuous study on the antioxidant activity by the extract of seaweed, which is safe for toxicity by routinely edible, and can be collected in large amounts on the east coast, confirming that the kelp extract exhibits a strong antioxidant effect, thereby completing the present invention. Was done.

In order to accomplish the above object, the present invention provides a pharmaceutical composition for the prevention and treatment of oxidative stress-related diseases containing kelp extract having antioxidant activity as an active ingredient.

The present invention also provides a health functional food for the prevention and improvement of oxidative stress-related diseases containing kelp extract having antioxidant activity as an active ingredient.

The present invention also provides a food additive containing the kelp extract having antioxidant activity as an active ingredient.

The present invention also provides an antioxidant containing the kelp extract having antioxidant activity as an active ingredient.

The present invention also provides a cosmetic composition containing the kelp extract having antioxidant activity as an active ingredient.

Kelp as defined herein includes Laminaria japonica, Laminaria ochotensis, Laminaria religiosa, preferably Laminaria japonica.

Extracts defined herein are specifically polar solvent extracts, which include water containing purified water, lower alcohols of C ₁ to C ₄ or mixed solvents thereof, preferably water, ethanol or mixed solvents thereof, more preferably Extracts soluble in 70-99% ethanol.

Oxidative stress related diseases as defined herein are specifically lipid metabolism diseases such as hyperlipidemia; Heart diseases such as atherosclerosis, heart failure, hypertensive heart disease, arrhythmia myocardial infarction and angina pectoris; Skin aging such as wrinkles, blemishes and freckles, immune diseases and cancer, preferably skin aging such as wrinkles, blemishes and freckles.

Hereinafter, the present invention will be described in detail.

Kelp extract of the present invention can be obtained as follows.

The extract of the present invention comprises the first step of slicing and pulverizing the dried natural Laminaria japonica; Water containing about 1 to 50 times (w / v), preferably about 1 to 5 times (w / v) purified water, C ₁ to C ₄ lower alcohols or mixed solvents thereof, preferably in each comminute Is a polar organic solvent such as water, ethanol or a mixed solvent thereof, more preferably 70 to 99% ethanol, and is about 0 ° C to 100 ° C, preferably about 20 ° C to 70 ° C, and more preferably about 40 ° C. A second step of extracting by stirring extraction, hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction for about 10 minutes to 1 hour, preferably 20 to 40 minutes at a temperature, preferably ultrasonic extraction; The extract of the present invention may be obtained through a drying step of drying the extract of the second step, such as filtration, concentration under reduced pressure, and lyophilization.

The present invention provides a pharmaceutical composition for the prevention and treatment of oxidative stress-related diseases containing the kelp extract obtained by the above manufacturing process having an antioxidant activity as an active ingredient.

In another aspect, the present invention provides a health functional food for the prevention and improvement of oxidative stress-related diseases containing the kelp extract obtained by the above manufacturing process having an antioxidant activity as an active ingredient.

The present invention also provides a food additive containing the kelp extract obtained by the above manufacturing process having an antioxidant activity as an active ingredient.

The present invention also provides an antioxidant containing the kelp extract obtained by the above manufacturing process having an antioxidant activity as an active ingredient.

The present invention also provides a cosmetic composition containing the kelp extract obtained by the above manufacturing process having an antioxidant activity as an active ingredient.

As a result of measuring the free radical scavenging ability of the kelp extract obtained by the above method, it showed a stronger antioxidant effect than the synthetic antioxidant BHA.

In particular, the composition comprises a pharmaceutical composition comprising a pharmaceutically acceptable carrier and excipients.

In addition, kelp is a drug that has been used for a long time as an edible extract of the present invention extracted therefrom also has no problems such as toxicity and side effects.

The pharmaceutical composition of the present invention comprises the extract in an amount of 0.02 to 50% by weight based on the total weight of the composition. Pharmaceutical compositions comprising the extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Pharmaceutical dosage forms of the extracts of the present invention may be used in the form of their pharmaceutically acceptable salts, or may be used alone or in combination with other pharmaceutically active compounds, as well as in any suitable collection.

Pharmaceutical compositions comprising extracts according to the invention, in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterile injectable solutions, respectively, according to conventional methods. Can be formulated and used. Examples of carriers, excipients and diluents that can be included in the composition containing the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The composition of the present invention provides a health functional food for the prevention and improvement of oxidative stress-related diseases comprising the kelp extract obtained according to the preparation method as an active ingredient.

In another aspect, the present invention provides a dietary supplement comprising a kelp extract exhibiting an antioxidant effect and a food supplement acceptable food supplement. Examples of foods to which kelp extract can be added include various foods, beverages, gums, teas, vitamin complexes, and health functional foods.

It may also be added to foods or beverages for the purpose of antioxidant effects. At this time, the amount of the extract in the food or beverage may be added in 0.01 to 15% by weight of the total food weight, the health beverage composition may be added in a ratio of 0.02 to 5g, preferably 0.3 to 1g based on 100ml. .

The health functional beverage composition of the present invention is not particularly limited to other ingredients except for containing the extract as an essential ingredient in the indicated proportions, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the extract of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the extracts of the present invention may contain flesh for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is usually selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the extract of the present invention.

The composition of the present invention provides a cosmetic composition for inhibiting and improving wrinkle formation comprising the kelp extract obtained according to the preparation method as an active ingredient.

The composition containing the extract of the present invention can be used in various ways, such as cosmetics, face wash and shampoo for the skin anti-aging effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, and skins, and shampoos, rinses, cleansing agents, face washes, soaps, treatments, and cosmetic liquids.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract. Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The anti-aging cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, emulsion, cream, lotion, pack, foundation, lotion, essence, hair cosmetics and the like.

The kelp extract of the present invention shows a potent free radical scavenging effect in the DPPH radical scavenging experiment, and has been found to enhance antioxidant activity, and thus can be used in pharmaceutical compositions and health functional foods useful for the prevention and treatment of oxidative stress. .

Hereinafter, the present invention will be described in detail with reference to the following examples and experimental examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Experimental Examples.

Example  1. Preparation of Kelp Ethanol Extract

Kelp (Laminaria) was dried in the East Sea as Laminaria japonica and purchased at Jumunjin Fish Market.

To 100 g of dried kelp dried powder prepared above, 95% ethanol was added at a ratio of 1: 5 (w / v), mixed, and then ultrasonically extracted at 30 ° C. for 30 minutes (power SONIC 520 HWASHIN). The extracted extract was filtered through a filter paper (1 mm-pore-size filter, Whatman No. 2) to obtain an ethanol extract. The resulting extract was concentrated under reduced pressure to remove ethanol at 40 ° C. with a rotary depressurizer (Rotavator; R-200, Buchi, switzerland) and freeze-dried with a lyophilizer (PVIFD30A, Ilsin Lab, Korea). , “LJ95E”) was obtained, which was stored at −40 ° C. or lower.

Experimental Example  1. Extraction yield measurement

Yield of the extract obtained in the above Example was calculated as a percentage of the total solid content of the solid solution (Soluble Solid) of the extract to the dried material of the sample used for extraction, the yield is calculated by the following formula 1 and the results are shown in Table 1 below It was.

As a result of the experiment, the extraction yield obtained by extracting a powder sample of kelp with ethanol was measured. As shown in Table 1, the ethanol extraction yield of kelp was 1.76%.

Solvent (v / w) Extraction Yield (%) 95% ethanol 1.76 * Unit: g / 100g

Experimental Example  2. Antioxidant Activity Measurement

To determine the in vitro antioxidant effect of kelp extract, DPPH method disclosed in the literature was applied and tested as follows (Blois, MS: Antioxidant determination by the use of a stable free radical, Nature, 26 , pp.1199 -1200, 1958).

DPPH (1,1-diphenyl picrylhydrazyl) method is a biological activity showing the antioxidant activity of tocopherol, ascorbate, flavonoid compounds, aromatic amines, Maillard browning products, peptides, etc. It is a method of measuring the antioxidant effect by the hydrogen donating ability according to the degree to which the dark purple color is reduced by being reduced by the substance.

2-1. Reagent Preparation

DPPH (α, α-diphenyl-β-picrylhydrazyl; SIGMA), a reagent for measuring antioxidant activity, BHA (Butylated hydroxyanisole; SIGMA) and grape seed oil (Ottogi Corp.) were purchased and used as a control.

2-2. Preparation of DPPH (α, α-diphenyl-β-picrylhydrazyl) Sample

DPPH sample preparation was used by dissolving the extract in ethanol and distilled water (1: 5: 4), dissolved in ethanol and distilled water (5: 4) to a concentration of 0.1, 1, 10, and 100 ㎍ / ml and DPPH 3.94 mg. It was dissolved in 25 ml of ethanol to make a DPPH solution.

2-3. DPPH radical scavenging activity measurement

Spectrophotometer (Ultraviolet-visible spectrophotometer Jasco.JP/V-550) was used to measure the extent of DPPH radical reduction using DPPH (α, α-diphenyl-β-picrylhydrazyl), a stable free radical as a model of unsaturated fatty acid radicals. Indirectly, the antioxidant activity of the sample was measured.

1 ml of the DPPH solution prepared in Experimental Example 2-1 was added to 250 µl of the sample solution, followed by shaking for 10 seconds, followed by centrifugation (microcentrifuge; Mega 21R) for 3 minutes at 15 ° C. at 6,000 × g. And left at room temperature for 30 minutes. Advance by an ethanol solution as a blank to measure the absorbance of the mixture after adjusting the zero point of the spectrophotometer (Bios, MS, Antioxide determination by the use of a stable free radical, Nature. 4617, pp.1198, 1958).

The absorbance at 517 nm was measured by spectrophotometer to calculate DPPH radical scavenging ability using Equation 2 below, the same amount of ethanol was added to the blank of the sample instead of the DPPH solution, and the same amount of ethanol was used as a control instead of the sample solution. It was measured by adding and repeating three times the averaged results were calculated by the following equation 2 to obtain the DPPH scavenging capacity (%) value.

C: Control

Sb: Sample

Ss: Sample blank

Experimental Example  3. Determination of Free radical scavenging effect of kelp ethanol extract by concentration

DPPH (α, α-diphenyl-β-picrylhydrazyl), used for the measurement of free radical scavenging effect, is a stable free radical, exhibiting a maximum absorbance near 517 nm due to its unshared electrons and a decrease in absorbance near 517 nm upon receipt of electrons or hydrogen. In addition, if each extract has a high ability to reduce or offset such radicals, it can be expected to have high antioxidant activity and scavenging activity against other radicals including active oxygen, and can also be used as a measure of inhibiting aging by active radicals in the human body. Shin YS. Et al., Antioxidant and antimicrobial Effects of extract with water and ethanol of oriental melon (Cucumismelo L. varmakuwa Makino), J Korean Soc Appl Biol Chem., 51 (3) , pp.194-199, 2008).

As a positive control, synthetic antioxidant BHA (Butylated hydroxyanisole) and grapeseed oil were used, and the kelp extract was treated at various concentrations, that is, 0.1, 1, 10, and 100 µg / ml, and the free radical scavenging effect was Confirmed.

As a result, as shown in Table 2, the kelp extract 100g / ㎖ concentration group showed a scavenging effect (86.84%), lower than the 100 ㎍ / ㎖ concentration group of grapeseed oil (93.00%) but BHA 100 The free radical scavenging effect was higher than that of the treatment group (75.58%). The kelp ethanol extract was also higher when compared to BHA (-4.42%) and grapeseed oil (-4.75%) at the same concentration (1.88%). It was confirmed.

Sample DPPH radical scavenging effect (%) (Mean ± SD)   0.1 µg / ml      1 µg / ml      10 µg / ml      100 µg / ml   LJ95E 1.21 ± 0.52 1.18 ± 1.07  9.25 ± 1.14 86.84 ± 0.30 BHA -5.13 ± 2.44 -4.42 ± 2.42 1.51 ± 2.29 75.58 ± 0.46 Grape seed oil -6.20 ± 2.40 -4.75 ± 2.40 18.51 ± 2.40 93.00 ± 2.14

In addition, the amount of DPPH required to inhibit the antioxidant activity result obtained by 50% in terms of IC ₅₀ , this activity measurement mode is shown in Figure 1 and Table 3.

Sample IC ₅₀ value (µg / mL) (Mean ± SD) LJ95E 60.39 ± 1.25 BHA 71.92 ± 1.77 Grape seed oil 51.05 ± 1.13

As a result, as shown in Table 3 and Figure 1, it was confirmed that the free radical scavenging effect of kelp ethanol extract has a greater effect from a constant concentration 100㎍ / ㎖. In addition, it showed a strong antioxidant effect compared to the antioxidant capacity of the synthetic antioxidant BHA. Therefore, kelp ethanol extract may be useful as an antioxidant natural food additive material as well as an antioxidant health functional food material.

Hereinafter, an example of the preparation of a pharmaceutical composition comprising the extract of the present invention will be described, but the present invention is not intended to be limited thereto, but is intended to be described in detail.

Formulation Example 1 Preparation of Powder

LJ95E 20 mg

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2 Preparation of Tablet

LJ95E 10 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components and tableting according to the conventional tablet manufacturing method to prepare a tablet.

Formulation Example 3 Preparation of Capsule

LJ95E 10 mg

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation Example 4 Preparation of Injection

LJ95E 10 mg

180 mg mannitol

Sterile distilled water for injection 2974 mg

Na ₂ HPO ₄ , 12H ₂ O 26 mg

According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule (2 ml).

Formulation Example 5 Preparation of Liquid

LJ95E 20 mg

10 g of isomerized sugar

5 g of mannitol

Purified water

After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding lemon flavor appropriately, mixing the above components, adding purified water, adjusting the whole to 100 ml by adding purified water, and then filling into a brown bottle. The solution is prepared by sterilization.

Hereinafter, an example of manufacturing a health food comprising the extract of the present invention will be described, but the present invention is not intended to be limited thereto but is intended to be described in detail.

Formulation Example 6 Preparation of Healthy Food

LJ95E 1000 mg

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

0.13 mg vitamin B1

0.15 mg of vitamin B2

0.5 mg vitamin B6

0.2 [mu] g vitamin B12

10 mg vitamin C

Biotin 10 μg

Nicotinic acid amide 1.7 mg

50 ㎍ of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

15 mg of potassium phosphate monobasic

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Formulation example  7. Manufacture of health drinks

LJ95E 1000 mg

Citric acid 1000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added to a total of 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated at 85 DEG C for about 1 hour with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, ≪ / RTI >

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

Hereinafter, a description will be given of an example of preparing a cosmetic including an extract of the present invention, but the present invention is not intended to be limited thereto but merely to be described in detail.

Formulation example  1. Preparation of Hair Tonic

Resorcinol 0.1%

Menthol 0.05%

Panthenol 0.2%

Salicylic Acid 0.1%

Tocopheryl Acetate 0.1%

Pyridoxine hydrochloride 0.1%

Castor Oil 5.0%

LJ95E 10.0%

Pigment amount

Spices

Ethanol

Purified water level

Total 100.0%

Formulation example  2. Of hair conditioner  Produce

Cetanol 3.5%

Self-emulsifying glycerin monostearate 1.5%

Propylene Glycol 2.5%

Stearylmethylbenzyl Ammonium Chloride (25%) 7.0%

0.3% methyl paraoxybenzoate

LJ95E 2.5%

Pigment amount

Spices

Purified water level

Total 100.0%

Formulation example  3. Hair lotion  Produce

Resorcinol 2.0%

Menthol 2.0%

Panthenol 0.5%

Pyroctonolamine 0.1%

LJ95E 5.0%

Fragrance, pigment 0.5%

Purified water level

Total 100.0%

Formulation example  4. Hair soap  Produce

LJ95E 0.1%

Titanium dioxide 0.2%

Polyethylene Glycol 0.8%

Glycerin 0.5%

Ethylenediaminetetraacetic Acid 0.05%

Sodium 1.0%

Pigment amount

Soap flavor

Cosmetic soap base (13% moisture, parts by weight)

Total 100.0%

[National R & D project supporting this invention]

[Task unique number]

F20815508H220000110 / RRC00200-0000-00 / B0010517-2008-01 / R01-2007-000-20704-0

[Name of Buddha]

Korea Ocean Fisheries Technology Development Institute / Ministry of Knowledge Economy / Ministry of Knowledge Economy / Korea Science Foundation

[Name of research project]

Fisheries-specific R & D industry / regional cooperation center project / regional industry promotion project / specific basic research

[Name of Research Project]

Application of high-quality processed fish products of essence oil as a high quality additive and commercialization of functional materials / Performance utilization business of East Coastal Marine Biological Resources Research Center / Sokcho Wellbeing Salted Fish Product Development Project And functional materialization

[Host]

Gangneung-Wonju National University / Gangneung-Wonju National University / Gangneung-Wonju National University / Gangneung-Wonju National University

[Research period]

December 01, 2008 ~ November 30, 2011 / January 01, 2009 ~ December 30, 2009 / July 01, 2008 ~ June 30, 2011 / September 01, 2007 ~ 2010 August 31,

1 is a diagram showing the DPPH free radical scavenging effect of kelp ethanol extract.

Claims (9)

A pharmaceutical composition for the prevention and treatment of oxidative stress-related diseases containing kelp extract having antioxidant activity as an active ingredient. The pharmaceutical composition according to claim 1, wherein the extract is a polar solvent extract. The pharmaceutical composition according to claim 2, wherein the extract is an extract available in water, ethanol or a mixed solvent thereof. According to claim 1, wherein the oxidative stress-related diseases are lipid metabolism diseases such as hyperlipidemia; Heart diseases such as atherosclerosis, heart failure, hypertensive heart disease, arrhythmia myocardial infarction and angina pectoris; Pharmaceutical composition which is skin aging such as wrinkles, blemishes, freckles, immune diseases or cancer. Health functional food for the prevention and improvement of oxidative stress-related diseases containing kelp extract with antioxidant activity as an active ingredient. A dietary supplement according to claim 5 which is a powder, granule, tablet, capsule or beverage. Food additive containing an extract of kelp with antioxidant activity as an active ingredient. Antioxidant containing kelp extract having antioxidant activity as an active ingredient. Cosmetic composition containing a kelp extract having antioxidant activity as an active ingredient.

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