KR102632060B1 - Composition for preventing alopesia comprising germinated black rice extracts - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss comprising a complex extract of sprouted black rice and fermented pork potatoes. The complex extract of sprouted black rice and fermented pork potatoes according to the present invention has excellent efficacy in promoting the proliferation and differentiation of dermal papilla cells, thereby preventing hair loss. It can be useful as a preventive and hair growth promoting functional composition.

Description

Composition for preventing hair loss comprising germinated black rice and fermented pork potato complex extracts {Composition for preventing alopesia comprising germinated black rice extracts}

The present invention relates to a composition for preventing hair loss comprising a complex extract of sprouted black rice and fermented pork potato.

Hair is formed when hair follicles, which are appendages to the skin, begin to form. Hair follicles are small organs that exist on the body surface of mammals and repeat changes in a certain cycle. The hair follicle cycle is divided into anagen, catagen, and telogen. In the anagen phase, hair grows as hair matrix cells and melanin-forming cells proliferate, and in the catagen phase, hair growth ends and the epithelial membrane With contraction, blood supply is stopped, and during the resting phase, the hair gradually pushes up to the pore area and the hair falls out.

According to the above-mentioned hair follicle cycle, immunological and hormonal changes around the hair follicle are accompanied. Stimulating factors such as psychological stress, lack of nutrition, drug side effects, and aging can cause hair loss by shortening the growth phase of the hair follicle and early progression of the catagen phase. You can.

Since hair is a part of one's appearance and plays an important role in improving one's public image, the importance of research on hair growth, hair loss, and hair loss prevention has recently been increasing. In particular, as lifestyle patterns become westernized, quality of life improves, and stress increases, concerns such as hair loss and scalp disease are becoming more of a concern for everyone. The causes are simple genetic factors and acquired factors, and the causes are simple. Importance and interest are gradually growing. Since hair loss can cause psychological withdrawal, which can lead to avoidance of interpersonal relationships and loss of confidence, it can have a significant impact on maintaining an individual's prosperous life, and the hair loss industry market is continuously growing rapidly.

Currently, representative hair loss treatments approved by the U.S. Food and Drug Administration (FDA) include minoxidil for application and Propecia for oral use, which are synthetic drugs, and several side effects are continuously reported. Propecia is effective by inhibiting the process of converting male hormone (testosterone) to dihydrotestosterone (DHT), the causative agent of hair loss, but concerns have been raised that it may reduce men's libido, and minoxidil was developed as a treatment for high blood pressure, but causes hair follicle activation. It was developed as a hair growth agent as it was reported to promote hair growth, but side effects such as various skin diseases have been reported.

Research on hair loss prevention and hair growth promotion using various natural products is actively being conducted, but commercialization of hair growth promoters or hair loss inhibitors is still minimal due to their lower activity compared to pharmaceuticals.

Accordingly, the present inventor confirmed that a mixture of sprouted black rice and fermented pork potato extract was excellent in promoting the proliferation and differentiation of dermal papilla cells and completed the present invention.

Republic of Korea Patent Publication No. 10-0778662

The purpose of the present invention is to provide a cosmetic composition for preventing hair loss or promoting hair growth.

Another object of the present invention is to provide a health food composition for preventing hair loss or promoting hair growth.

Another object of the present invention is to provide a health functional food composition for preventing hair loss or promoting hair growth.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating hair loss.

In order to achieve the above purpose,

The present invention provides a cosmetic composition for preventing hair loss or promoting hair growth comprising a complex extract of sprouted black rice and fermented pork potato.

The cosmetics may be in the form of lotion, emulsion, cream, skin, lotion, serum, essence, emulsion, powder, cosmetic ointment, spray, gel, pack, cleanser, soap, shampoo, rinse, bath salt, detergent, or concealer stick. It is not limited to this.

In addition, the present invention provides a health food composition for preventing hair loss or promoting hair growth comprising a complex extract of sprouted black rice and fermented pork potato.

The health food may be in the form of drinks, meat, sausages, bread, candies, snacks, noodles, ice cream, dairy products, soups, isotonic drinks, beverages, alcoholic beverages, gum or tea, but is not limited thereto.

Furthermore, the present invention provides a health functional food composition for preventing hair loss or promoting hair growth comprising a complex extract of sprouted black rice and fermented pork potato.

The health functional food may be in the form of tablets, capsules, pills, or liquid, but is not limited thereto.

Additionally, the present invention provides a pharmaceutical composition for preventing or treating hair loss comprising a complex extract of sprouted black rice and fermented pork potato.

The pharmaceutical composition may preferably be in the form of an external application to the skin, and may also be applied as an oral administration agent.

Since the complex extract of sprouted black rice and fermented pork potato according to the present invention has excellent efficacy in promoting proliferation and differentiation of dermal papilla cells, it can be useful as a functional composition for preventing hair loss and promoting hair growth.

Figure 1 is an image confirming the degree of restoration of scratches artificially applied to dermal dermal papilla cells according to treatment in Example 1 (complex extract of germinated black rice and fermented pork potato) and Comparative Example 1 (minoxidil, 'MIX').
Figure 2 is an image confirming the expression level of Alkaline Phosphatase in dermal dermal papilla cells according to treatment in Example 1 (germinated black rice extract) and Comparative Example 1 (minoxidil, 'MIX').

Hereinafter, the present invention will be described in detail.

cosmetic composition

The present invention provides a cosmetic composition containing an active substance.

The cosmetic compositions include, for example, solutions, gels, solid or pasty anhydrous products, emulsions obtained by dispersing the oil phase in the water phase, suspensions, microemulsions, microcapsules, microgranules, or ionic (liposome) and non-ionic vesicular dispersants. It may be in the form of . More specifically, it is provided in the form of lotion, emulsion, cream, skin, lotion, serum, essence, emulsion, powder, cosmetic ointment, spray, gel, pack, cleanser, soap, shampoo, rinse, bath salt, cleanser, or concealer stick. It can be. Additionally, it can be manufactured in the form of foam or in the form of an aerosol composition further containing compressed propellant.

In addition to the active substances of the present invention, the cosmetic composition contains fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Commonly used in water, ionic or non-ionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or in cosmetics. It may contain auxiliaries commonly used in the cosmetic field, such as any other ingredients.

In a cosmetic composition containing the active substance of the present invention, the active substance of the present invention may be added in an amount of 0.1 to 50% by weight, preferably 1 to 10% by weight, in a cosmetic composition that is usually contained.

When the active substance of the present invention is used as an external skin agent, fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, and surfactants are added. , water, ionic or non-ionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, blocking agents, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or in external preparations for the skin. It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients used. Additionally, the ingredients may be introduced in amounts commonly used in the field of dermatology.

Health food and health functional food composition

There are no special restrictions on the type of food, and it includes both health foods and health functional foods in the usual sense.

Examples of health foods include drinks, meat, sausages, bread, candies, snacks, noodles, ice cream, dairy products, soups, isotonic drinks, beverages, alcoholic beverages, gum, and tea.

Examples of health functional foods include health functional foods in the form of tablets, capsules, pills, and liquids.

Health food and health functional food compositions containing the active substances according to the present invention can be added as is to food or used together with other foods or food ingredients, and can be used appropriately according to conventional methods. The mixing amount of the effective substance can be appropriately determined depending on the purpose of use (prevention or improvement). In general, the amount of the composition in health foods and health functional foods can be 0.1 to 90 parts by weight of the total weight of the food. However, in the case of long-term intake for the purpose of maintaining health or regulating health, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

The health food and health functional food composition of the present invention has no particular restrictions on other ingredients other than containing the active substance of the present invention as an essential ingredient in the indicated ratio, and, like a typical beverage, various flavoring agents or natural carbohydrates are added as additional ingredients. It may contain. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose, etc.; Disaccharides such as maltose, sucrose, etc.; and polysaccharides, such as common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (thaumatin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g, per 100 of the health functional food composition of the present invention.

In addition to the above, health food and health functional food compositions containing the active substances of the present invention include various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, colorants, and additives (cheese, chocolate, etc.) ), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. In addition, the health food and health functional food composition of the present invention may contain pulp for the production of natural fruit juice, fruit juice drinks, and vegetable drinks.

These ingredients can be used independently or in combination. The ratio of these additives is not that important, but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the health food or health functional food composition containing the active substance of the present invention.

pharmaceutical composition

The active substance of the present invention can be administered in various oral and parenteral dosage forms during clinical administration, and when formulated, diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants are used. It is manufactured.

Solid preparations for oral administration include tablets, tablets, powders, granules, capsules, troches, etc. These solid preparations include one or more active substances of the present invention and at least one or more excipients, such as starch, calcium carbonate, It is prepared by mixing sucrose, lactose, or gelatin. Additionally, in addition to simple excipients, lubricants such as magnesium styrate talc are also used. Liquid preparations for oral administration include suspensions, oral solutions, emulsions, or syrups. In addition to the commonly used simple diluents such as water and liquid paraffin, they contain various excipients such as wetting agents, sweeteners, fragrances, and preservatives. You can.

Preparations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, suppositories, etc. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween 61, cacao, laurel, glycerol, gelatin, etc. can be used.

In addition, the effective dosage of the active substance of the present invention to the human body may vary depending on the patient's age, weight, gender, dosage form, health condition, and disease level, and is generally about 0.001-100 mg/kg/day. Preferably it is 0.01-35 mg/kg/day. Based on an adult patient weighing 70 kg, the dose is generally 0.07-7000 mg/day, preferably 0.7-2500 mg/day, and is administered once a day at regular intervals depending on the judgment of the doctor or pharmacist. It may be administered in several divided doses.

external skin preparation

Ingredients included in the composition of the present invention may include ingredients commonly used in external skin compositions in addition to the above active ingredients, such as conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and May include carriers, excipients and/or diluents. Examples of carriers, excipients and diluents suitable for formulation include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, malditol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, or mineral oil may be used.

When applied to pharmaceuticals, commonly used inorganic or organic carriers can be added to formulate parenteral administration in solid, semi-solid or liquid form.

In order to formulate the active ingredient of the present invention, it can be easily formulated by carrying out conventional methods, and surfactants, excipients, colorants, spices, preservatives, stabilizers, buffers, suspending agents, and other commercially available auxiliaries can be appropriately used.

The composition for topical skin application of the present invention can be prepared in any formulation commonly prepared in the art, for example, in the form of ointment, patch, gel, cream or spray, but is not limited thereto. .

Hereinafter, the present invention will be described in more detail through the following examples. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following examples.

<Example 1> Preparation of complex extract of sprouted black rice and fermented pork potato

100g of 'germinated' black rice was added to 1L of 70% ethanol and extracted three times at room temperature for 24 hours to obtain an extract. The extract was filtered and concentrated, then freeze-dried and powdered.

Biological Resource Center KCCM 12116 Lactibacillus plantarum strain was purchased, cultured on MRS Agar medium, inoculated at 5% V/V into 400ml of dried ground pork potato powder, 70% EtOH, 80℃, 2DAY, hot water concentrate, and placed in an incubator at 37℃ for 48 hours. By culturing the pig potato lactic acid bacteria bioconversion result, it was freeze-dried and used as a sample.

The germinated black rice extract sample and the fermented pork potato sample were mixed at a weight ratio of 1:1 to prepare a complex extract of the sprouted black rice and fermented pork potato.

<Comparative Example 1> Minoxidil

As Comparative Example 1, minoxidil (hereinafter referred to as 'MIX') was prepared.

<Comparative Example 2> Preparation of non-germinated regular black rice extract

100g of ungerminated 'normal' black rice was added to 1L of 70% ethanol and extracted repeatedly three times for 24 hours at room temperature. The extract was filtered and concentrated, then freeze-dried and powdered.

<Comparative Example 3> Germinated black rice ( Oryza sativa L.) Preparation of extract

100g of 'germinated' black rice was added to 1L of 70% ethanol and extracted three times at room temperature for 24 hours to obtain an extract. The extract was filtered and concentrated, then freeze-dried and powdered.

<Experimental Example 1> Evaluation of the efficacy of promoting proliferation and differentiation of dermal dermal papilla cells (Scratch test)

Control: A group in which no treatment was performed on human dermal papilla cells (HDPCs).

Negative control: A group in which stress was induced in human hair papilla cells by treatment with H ₂ O ₂ and then no treatment was performed.

Positive control: A group in which stress was induced in dermal papilla cells by treatment with H ₂ O ₂ and then the human dermal papilla cells were treated with Comparative Example 1 (minoxidil, 'MIX')

Experimental group: A group in which stress was induced in dermal papilla cells by treatment with H ₂ O ₂ and then the human dermal papilla cells were treated with Example 1 (sprouted black rice and fermented pork potato complex extract) and Comparative Example 3 (sprouted black rice extract).

After inoculating human dermal papilla cells in a 6-well plate, 2 days later, each well was scraped with a 100μL sterilized tip, and the above three groups, excluding Control, were treated with H ₂ O ₂ and then performed with Comparative Example 1 (minoxidil, 'MIX') After treatment of Example 1 (sprouted black rice and fermented pork potato complex extract) and Comparative Example 3 (sprouted black rice extract), the scratched portion was photographed at 0 hr and 24 hr.

Figure 1 shows the degree of restoration of scratches artificially applied to dermal dermal papilla cells according to treatment of Example 1 (complex extract of germinated black rice and fermented pork potato), Comparative Example 1 (minoxidil, 'MIX'), and Comparative Example 3 (germinated black rice extract). This is a confirmed image.

As shown in Figure 1, the group treated with 50 nM of Comparative Example 1 (minoxidil, 'MIX') and the group treated with 10 ppm of Example 1 (sprouted black rice and fermented pork potato complex extract) showed that most of the wounds were recovered 24 hours after scratching. It was confirmed that the sprouted black rice extract and the complex extract of germinated black rice and fermented pork potato can promote the proliferation and differentiation of dermal hair papilla cells, and the results of Example 1 (complex extract of sprouted black rice and fermented pig potato) were compared to the comparative example. It was confirmed that the degree of cell aggregation was higher than that of 3 (germinated black rice extract) and that proliferation and differentiation were promoted.

<Experimental Example 2> Evaluation of the efficacy of promoting Alkaline Phosphatase expression in dermal hair papilla cells

The experimental group and control group were configured in the same manner as in Experimental Example 1 above.

Specifically, excluding the control, the Negative control, Positive control, and experimental groups were treated with H ₂ O ₂ to induce stress in the dermal papilla cells, the Negative control was not treated with anything, and the Positive control was Comparative Example 1 (minoxidil, 'MIX') ) and finally, the experimental group was treated with a fixative solution (4% paraformaldehyde) for 5 minutes 48 hours after treatment with Example 1 (sprouted black rice and fermented pork potato complex extract). Control treated the fixation solution without any treatment.

After removing the fixing solution from each group, each group was treated with alkaline phosphatase substrate, and after a certain period of time, the stained cells were washed with distilled water and stained blue under a microscope. cells were confirmed.

Figure 2 is an image confirming the expression level of Alkaline Phosphatase in dermal dermal papilla cells according to treatment in Example 1 (complex extract of germinated black rice and fermented pork potato) and Comparative Example 1 (minoxidil, 'MIX').

As shown in Figure 2, it can be seen that the expression level of Alkaline Phosphatase is significantly reduced in the hydrogen peroxide (H ₂ O ₂ ) treated group compared to the normal control group (Control). Alkaline Phosphatase expression was restored to the normal control level in both the 50nM treatment group of Comparative Example 1 (minoxidil, 'MIX') and the 10ppm treatment group of Example 1 (germinated black rice and fermented pork potato complex extract). It was confirmed that the complex extract of sprouted black rice and fermented pork potato can promote the proliferation and differentiation of dermal dermal papilla cells.

Manufacturing example of cosmetics

The active substance according to the present invention can be manufactured into various types of cosmetics depending on the purpose. The following is an example of a manufacturing method of some cosmetics containing the active substance according to the present invention as an active ingredient, and the present invention is not limited thereto.

<Cosmetic Preparation Example 1> Preparation of flexible lotion

Active substance 10.0 parts by weight

1,3-butylene glycol 1.00 parts by weight

Disodium EDTA 0.05 parts by weight

Allantoin 0.10 parts by weight

Dipotassium glycyrrhizate 0.05 parts by weight

Citric acid 0.01 parts by weight

Sodium citrate 0.02 parts by weight

Glycereth-26 1.00 parts by weight

Arbutin 2.00 parts by weight

Hydrogenated castor oil 1.00 parts by weight

30.0 parts by weight of ethanol

Trace amount of preservative

Trace amount of colorant

Trace amount of flavoring agent

Purified water remaining

<Cosmetic Preparation Example 2> Preparation of nutritional cream

Active substance 10.0 parts by weight

7.00 parts by weight of 1,3-butylene glycol

Glycerin 1.00 parts by weight

D-Panthenol 0.10 parts by weight

3.20 parts by weight of plant extract

Magnesium aluminum silicate 0.30 parts by weight

PEG-40 stearate 1.20 parts by weight

Stearic acid 2.00 parts by weight

Polysorbate 60 1.50 parts by weight

Lipophilic glyceryl stearate 2.00 parts by weight

Sorbitan sesquioleate 1.50 parts by weight

Cetearyl alcohol 3.00 parts by weight

Mineral oil 4.00 parts by weight

Squalane 3.80 parts by weight

Carlylic/capric triglyceride 2.80 parts by weight

Vegetable oil 1.80 parts by weight

Dimethicone 0.40 parts by weight

Dipotium glycyrrhizate trace amount

Trace amounts of allantoin

Sodium Hyaluronate Trace

Tocopheryl acetate appropriate amount

Triethanolamine dosage

Preservative amount

Flavoring agent appropriate amount

Purified water remaining

Manufacturing example of health food

The active substance according to the present invention can be manufactured into various types of health foods depending on the purpose. The following is an example of a manufacturing method of some health foods containing the active substance according to the present invention as an active ingredient, and the present invention is not limited thereto.

<Health food manufacturing example 1> Manufacturing of dairy products

0.01-1 part by weight of the active substance of the present invention was added to milk, and various dairy products such as butter and ice cream were manufactured using the milk.

<Health food production example 2> Production of sun food

Brown rice, barley, glutinous rice, and coix seed were gelatinized and dried using a known method, roasted, and then made into powder with a particle size of 60 mesh using a grinder. Black beans, black sesame seeds, and perilla seeds were steamed and dried using a known method, roasted, and then made into powder with a particle size of 60 mesh using a grinder. The active substance of the present invention was concentrated under reduced pressure in a vacuum concentrator to obtain a dry powder. The dried powders of grains, seeds, and active substances prepared above were mixed in the following ratio.

Grains (34 parts by weight of brown rice, 19 parts by weight of coix radish, 20 parts by weight of barley),

Seeds (7 parts by weight perilla seeds, 8 parts by weight black beans, 7 parts by weight black sesame seeds),

Active substance (2 parts by weight),

Reishi (1.5 parts by weight), and

Rehmannia glutinosa (1.5 parts by weight).

Manufacturing example of health functional food

The active substance according to the present invention can be manufactured into various types of health functional foods depending on the purpose. The following is an example of a manufacturing method of some health functional foods containing the effective substance according to the present invention as an active ingredient, and the present invention is not limited thereto.

<Example 1 of manufacturing health functional food> Manufacturing of health functional food

Active substance 100 mg

Vitamin mixture dosage

Vitamin A acetate 70 μg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

Vitamin B12 0.2 μg

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg folic acid

Calcium pantothenate 0.5 mg

Mineral mixture appropriate amount

Ferrous sulfate 1.75 mg

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Monobasic Potassium Phosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

The composition ratio of the above vitamin and mineral mixture is a mixture of ingredients relatively suitable for health functional foods in a preferred embodiment, but the mixing ratio may be modified arbitrarily, and the above ingredients are mixed according to a typical health functional food manufacturing method. Then, granules can be prepared and used to manufacture health functional food compositions according to conventional methods.

<Example 2 of manufacturing health functional food> Manufacturing of health functional beverage

Active substance 100 mg

100 mg citric acid

100 mg of oligosaccharides

Plum concentrate 2 mg

Taurine 100 mg

Add purified water to make a total of 500 mL.

After mixing the above ingredients according to a typical health drink manufacturing method, stirring and heating at 85°C for about 1 hour, the resulting solution was filtered, obtained in a sterilized container, sealed, sterilized, and stored in the refrigerator. Then, the present invention It is used in the production of health drink compositions. The composition ratio is a preferred embodiment of mixing ingredients relatively suitable for preferred drinks, but the mixing ratio may be arbitrarily modified according to regional and ethnic preferences such as demand class, demand country, and use purpose.

Preparation example of medicine

The active substance according to the present invention can be formulated in various forms depending on the purpose. The following illustrates several formulation methods containing the effective substance according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Drug Preparation Example 1> Preparation of powder

2 g of active substance

1 g lactose

After mixing the above ingredients, they were filled into an airtight bubble to prepare a powder.

<Drug Preparation Example 2> Preparation of tablets

Active substance 100 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above ingredients, tablets were manufactured by tableting according to a conventional tablet manufacturing method.

<Drug Preparation Example 3> Preparation of capsules

Active substance 100 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above ingredients, a capsule was prepared by filling a gelatin capsule according to a typical capsule manufacturing method.

<Pharmaceutical Preparation Example 4> Preparation of injections

Active substance 10 ㎍/㎖

Dilute hydrochloric acid BP until pH 3.5

Sodium Chloride BP for Injection up to 1 ml

The active substance according to the present invention was dissolved in an appropriate volume of sodium chloride BP for injection, the pH of the resulting solution was adjusted to pH 3.5 using diluted hydrochloric acid BP, and the volume was adjusted using sodium chloride BP for injection and thoroughly mixed. . The solution was filled into a 5 ml Type I ampoule made of transparent glass, sealed under an upper grid of air by dissolving the glass, and sterilized by autoclaving at 120°C for 15 minutes or more to prepare an injection solution.

<Drug Preparation Example 5> Preparation of nasal absorbent (Nasal spray)

Active substance 1.0 g

Sodium acetate 0.3 g

Methylparaben 0.1 g

Propylparaben 0.02 g

Sodium chloride dosage

HCl or NaOH pH adjustment appropriate amount

Proper amount of purified water

According to the usual manufacturing method of a nasal absorbent, it is prepared to contain 3 mg of the active substance per 1 mL of saline water (0.9% NaCl, w/v, the solvent is purified water), filled into an opaque spray container, and sterilized to prepare a nasal absorbent. Manufactured.

<Drug Preparation Example 6> Preparation of liquid preparation

Active substance 100 mg

10 g isomerized sugar

5 g mannitol

Proper amount of purified water

According to the usual liquid preparation method, add and dissolve each ingredient in purified water, add lemon zest, mix the above ingredients, add purified water to adjust the total to 100 mL, fill in a brown bottle, and sterilize to prepare the liquid. did.

Manufacturing example of external skin preparation

The active substance according to the present invention can be formulated in various forms depending on the purpose. The following illustrates several formulation methods containing the effective substance according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Example 1 of preparation of external skin preparation> Preparation of spray

Active substance 100 mg

10 g isomerized sugar

5 g mannitol

Proper amount of purified water

According to a typical spray manufacturing method, add and dissolve each ingredient in purified water, add lemon scent, mix the above ingredients, add purified water to adjust the total to 100 mL, fill in a brown bottle, and sterilize to prepare the spray. did.

<Example 2 of preparation of external skin preparation> Preparation of gel

Active substance 100 mg

10 g of ethanol

4 g glycerin

4 g propylene glycol

0.1 g polyoxyethylene hydrogenated castor oil

Carboxypolymer 0.3 g

Triethanolamine 0.3g

Antioxidant dosage

Spice Proper Amount

Preservative dosage

Proper amount of purified water

According to a typical gel preparation method, each ingredient was added and dissolved in purified water, fragrance was added, and the above ingredients were mixed to prepare a gel.

<Example 3 of preparation of external skin preparation> Preparation of ointment

Active substance 100 mg

2 g cetostearyl alcohol

Self-emulsifying monostearic acid 2 g

1 g stearic acid

4 g beeswax

7 g of squalane

Monostearin glycerin 3 g

1 g sorbitan monostearate

Polysorbate 80 3 g

5 g glycerin

4 g propylene glycol

Spice Proper Amount

Vaseline remaining amount

According to a typical ointment manufacturing method, each ingredient was added and dissolved in vaseline, fragrance was added, and the above ingredients were mixed to prepare an ointment.

So far, the present invention has been examined focusing on its preferred embodiments. A person skilled in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a restrictive perspective. The scope of the present invention is set forth in particular in the claims rather than the foregoing description, and all differences within the equivalent scope should be construed as being included in the present invention.

Claims (8)

A cosmetic composition for preventing or improving hair loss containing a complex extract of sprouted black rice and fermented pork potato as an active ingredient,
The fermented pork potato is fermented with Lactobacillus plantarum KCCM 12116 strain,
The germinated black rice and fermented pork potatoes are mixed in a 1:1 weight ratio,
A composition wherein the complex extract of sprouted black rice and fermented pork potato promotes the proliferation or differentiation of dermal papilla cells and increases the expression of alkaline phosphatase.
According to clause 1,
The cosmetics are in the form of lotion, emulsion, cream, skin, lotion, serum, essence, emulsion, powder, cosmetic ointment, spray, gel, pack, cleanser, soap, shampoo, rinse, bath salt, detergent, or concealer stick. Cosmetic composition.
A food composition for preventing or improving hair loss containing a complex extract of sprouted black rice and fermented pork potato as an active ingredient,
The fermented pork potato is fermented with Lactobacillus plantarum KCCM 12116 strain,
The germinated black rice and fermented pork potatoes are mixed in a 1:1 weight ratio,
A composition wherein the complex extract of sprouted black rice and fermented pork potato promotes the proliferation or differentiation of dermal papilla cells and increases the expression of alkaline phosphatase.
According to clause 3,
A food composition characterized in that the food is in the form of drinks, meat, sausages, bread, candies, snacks, noodles, ice cream, dairy products, soups, isotonic drinks, beverages, alcoholic beverages, gum or tea.
A health functional food composition for preventing or improving hair loss containing a complex extract of sprouted black rice and fermented pork potato as an active ingredient,
The fermented pork potato is fermented with Lactobacillus plantarum KCCM 12116 strain,
The germinated black rice and fermented pork potatoes are mixed in a 1:1 weight ratio,
A composition wherein the complex extract of sprouted black rice and fermented pork potato promotes the proliferation or differentiation of dermal papilla cells and increases the expression of alkaline phosphatase.
According to clause 5,
The health functional food composition is characterized in that the health functional food is in the form of tablets, capsules, pills or liquid.
A pharmaceutical composition for preventing or treating hair loss containing a complex extract of sprouted black rice and fermented pork potato as an active ingredient,
The fermented pork potato is fermented with Lactobacillus plantarum KCCM 12116 strain,
The germinated black rice and fermented pork potatoes are mixed in a 1:1 weight ratio,
A composition wherein the complex extract of sprouted black rice and fermented pork potato promotes the proliferation or differentiation of dermal papilla cells and increases the expression of alkaline phosphatase.
According to clause 7,
A pharmaceutical composition, characterized in that the pharmaceutical composition is in the form of an external preparation for skin.