KR20230126811A - Composition for preventing alopesia comprising germinated black rice extracts - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss comprising an extract of germinated black rice, and since the extract of germinated black rice according to the present invention has an excellent effect of promoting proliferation and differentiation of dermal papilla cells, it can be useful as a functional composition for preventing hair loss and promoting hair growth. there is.

Description

Composition for preventing alopesia comprising germinated black rice extracts

The present invention relates to a composition for preventing hair loss comprising an extract of germinated black rice.

Hair is generated when hair follicles, an appendage of the skin, begin to form, and hair follicles are small organs that exist on the body surface of mammals and repeat changes according to a regular cycle. The hair follicle cycle is divided into anagen, catagen, and telogen. In the anagen phase, hair grows as the hair matrix cells and melanin-forming cells of the hair follicle proliferate, and in the catagen phase, hair growth ends and epithelial membrane The blood supply is stopped along with the contraction, and in the resting period, the hair is gradually pushed up to the pore area and the hair falls out.

According to the hair follicle cycle described above, immunological changes and hormonal changes around the hair follicle are accompanied, and stimulating factors such as psychological stress, lack of nutrition, drug side effects, and aging can cause hair loss by shortening the anagen phase and early progression of the catagen phase of the hair follicle. can

Since hair plays an important role in improving external image as a part of appearance, the importance of research on hair growth, hair loss, and hair loss prevention is increasing. In particular, as lifestyles become westernized, quality of life improves and stress increases, concerns such as hair loss and scalp diseases are expanding to everyone's concerns, and the causes are simple genetic and acquired factors. Its importance and interest are gradually increasing. Since hair loss can cause psychological shrinkage, which can lead to interpersonal avoidance and loss of self-confidence, it can have a great impact on maintaining an individual's affluent life, and the hair loss industry market continues to grow rapidly.

Representative hair loss treatments currently approved by the US Food and Drug Administration (FDA) include minoxidil for application and propecia for oral use as synthetic drugs, and several side effects have been continuously reported. Propecia works by suppressing the process of converting testosterone to dihydrotestosterone (DHT), the causative agent of hair loss, but problems have been raised that it can reduce male libido. It was developed as a hair growth agent as it was reported to promote hair growth through, but side effects such as various skin diseases have been reported.

Although studies on hair loss prevention and hair growth promotion using various natural substances are being actively conducted, commercialization of hair growth promoters or hair loss inhibitors is still insignificant due to their low activity compared to pharmaceuticals.

Accordingly, the present inventors confirmed that the germinated black rice extract had excellent efficacy in promoting the proliferation and differentiation of dermal papilla cells and completed the present invention.

Republic of Korea Patent Registration No. 10-0778662

An object of the present invention is to provide a cosmetic composition for preventing hair loss or promoting hair growth.

Another object of the present invention is to provide a health food composition for preventing hair loss or promoting hair growth.

Another object of the present invention is a health functional food composition for preventing hair loss or promoting hair growth

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating hair loss.

In order to achieve the above purpose,

The present invention provides a cosmetic composition for preventing hair loss or promoting hair growth comprising an extract of germinated black rice.

The cosmetics may be in the form of lotion, emulsion, cream, toner, lotion, serum, essence, emulsion, powder, cosmetic ointment, spray, gel, pack, cleanser, soap, shampoo, rinse, bath additive, cleanser, or conceal stick, Not limited to this.

In addition, the present invention provides a health food composition for preventing hair loss or promoting hair growth, including an extract of germinated black rice.

The health food may be in the form of drinks, meat, sausages, bread, candies, snacks, noodles, ice cream, dairy products, soups, ionic beverages, beverages, alcoholic beverages, gum or tea, but is not limited thereto.

Furthermore, the present invention provides a health functional food composition for preventing hair loss or promoting hair growth, including an extract of germinated black rice.

The health functional food may be in the form of tablets, capsules, pills or liquids, but is not limited thereto.

In addition, the present invention provides a pharmaceutical composition for preventing or treating hair loss comprising an extract of germinated black rice.

The pharmaceutical composition may preferably be in the form of an external preparation for the skin, and may also be applied as an oral preparation.

Since the germinated black rice extract according to the present invention has an excellent effect of promoting proliferation and differentiation of dermal papilla cells, it can be useful as a functional composition for preventing hair loss and promoting hair growth.

1 is an image confirming the degree of restoration of scratches artificially applied to dermal dermal papilla cells according to Example 1 (germinated black rice extract) and Comparative Example 1 (minoxidil, 'MIX') treatment.
Figure 2 is an image confirming the expression level of Alkaline Phosphatase in dermal dermal papilla cells according to Example 1 (germinated black rice extract) and Comparative Example 1 (minoxidil, 'MIX') treatment.

Hereinafter, the present invention will be described in detail.

cosmetic composition

The present invention provides a cosmetic composition containing an active substance.

The cosmetic composition is, for example, a solution, gel, solid or kneaded anhydrous product, emulsion obtained by dispersing an oil phase in an aqueous phase, suspension, microemulsion, microcapsule, microgranule or ionic (liposome), nonionic vesicle dispersant may be in the form of In more specific form, it is provided in the form of lotion, emulsion, cream, toner, lotion, serum, essence, emulsion, powder, cosmetic ointment, spray, gel, pack, cleanser, soap, shampoo, rinse, bath additive, cleanser, or conceal stick. It can be. In addition, it may be prepared in the form of a foam (foam) or the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition includes a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, Water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or commonly used in cosmetics It may contain adjuvants commonly used in the cosmetic field, such as any other ingredients that are

In the cosmetic composition containing the active material of the present invention, the active material of the present invention may be added in an amount of 0.1 to 50% by weight, preferably 1 to 10% by weight, in the cosmetic composition that is usually contained.

When the active substance of the present invention is used as an external skin preparation, additionally, a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, and a surfactant , water, ionic or nonionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles, or external preparations for skin It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients used as In addition, the components may be introduced in an amount generally used in the field of skin science.

Health food and health functional food composition

There is no particular limitation on the type of food, and includes both health food and health functional food in the usual sense.

Examples of health foods include drinks, meat, sausages, bread, candies, snacks, noodles, ice cream, dairy products, soups, ionic beverages, beverages, alcoholic beverages, gum, tea, and the like.

Examples of health functional foods include health functional foods in the form of tablets, capsules, pills, and liquids.

Health food and health functional food compositions containing active substances according to the present invention may be added to food as it is or used together with other foods or food ingredients, and may be appropriately used according to conventional methods. The mixing amount of the active substance may be appropriately determined according to its purpose of use (for prevention or improvement). In general, the amount of the composition in health food and health functional food may be added in an amount of 0.1 to 90 parts by weight based on the total weight of food. However, in the case of long-term intake for the purpose of health maintenance or health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount greater than the above range.

The health food and health functional food composition of the present invention is not particularly limited in other ingredients except for containing the active substance of the present invention as an essential ingredient in the indicated ratio, and various flavoring agents or natural carbohydrates are added as additional ingredients like conventional beverages. may contain Examples of the aforementioned natural carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrins, cyclodextrins, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (thaumatin, stevia extract (eg rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can advantageously be used. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 of the health functional food composition of the present invention.

In addition to the above, the health food and health functional food composition containing the active substances of the present invention are various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.) ), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, and the like. In addition, the health food and health functional food composition of the present invention may contain fruit flesh for preparing natural fruit juice, fruit juice beverages, and vegetable beverages.

These components may be used independently or in combination. The ratio of these additives is not so critical, but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the health food and health functional food composition containing the active substance of the present invention.

pharmaceutical composition

The active substance of the present invention can be administered in various oral and parenteral formulations during clinical administration, and when formulated, diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants are used. It is manufactured by

Solid preparations for oral administration include tablets, patients, powders, granules, capsules, troches, etc., and these solid preparations contain one or more active substances of the present invention and at least one excipient such as starch, calcium carbonate, It is prepared by mixing sucrose, lactose or gelatin. In addition to simple excipients, lubricants such as magnesium styrate and talc are also used. Liquid formulations for oral administration include suspensions, internal solutions, emulsions, or syrups. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included. can

Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspension solutions, emulsions, freeze-dried formulations, suppositories, and the like. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerol, gelatin, and the like may be used.

In addition, the effective dosage of the active substance of the present invention for the human body may vary depending on the patient's age, weight, sex, dosage form, health condition and disease degree, and is generally about 0.001-100 mg/kg/day, Preferably it is 0.01-35 mg/kg/day. Based on an adult patient weighing 70 kg, it is generally 0.07-7000 mg/day, preferably 0.7-2500 mg/day, and once or twice a day at regular intervals according to the judgment of a doctor or pharmacist. It may be divided into several doses.

External skin preparation

Ingredients included in the composition of the present invention may include ingredients commonly used in compositions for external application for skin in addition to the above active ingredients, for example, antioxidants, stabilizers, solubilizers, conventional adjuvants such as vitamins, pigments and fragrances, and Carriers, excipients and/or diluents may be included. Examples of carriers, excipients and diluents suitable for formulation include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, malditol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, or mineral oil may be used.

When applied to pharmaceuticals, it can be formulated as a parenteral drug in the form of a solid, semi-solid or liquid by adding a commonly used inorganic or organic carrier.

In order to formulate the active ingredient of the present invention, it can be formulated easily by conducting it according to a conventional method, and surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffers, suspending agents, and other commercially available adjuvants can be appropriately used.

The composition for external application for skin of the present invention may be prepared in any formulation commonly prepared in the art, for example, may be formulated in the form of an ointment, patch, gel, cream or spray, but is not limited thereto. .

Hereinafter, the present invention will be described in more detail by the following examples. However, the following examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

<Example 1> Germinated black rice ( Oryza sativa L.) Preparation of extracts

100 g of 'germinated' black rice was added to 1 L of 70% ethanol and repeatedly extracted 3 times for 24 hours at room temperature, and the extract was concentrated by filtration and lyophilized to powder.

<Comparative Example 1> Minoxidil

As Comparative Example 1, minoxidil (hereinafter referred to as 'MIX') was prepared.

<Comparative Example 2> Preparation of non-germinated black rice extract

100 g of ungerminated 'regular' black rice was added to 1 L of 70% ethanol, extracted three times for 24 hours at room temperature, and the extract was concentrated by filtration and lyophilized to powder.

<Experimental Example 1> Evaluation of proliferation and differentiation promotion efficacy of dermal dermal papilla cells (Scratch test)

Control: a group that did not receive any treatment to human dermal papilla cells (HDPCs)

Negative control: a group that did not receive any treatment after stress was induced in human dermal papilla cells by treatment with H ₂ O ₂

Positive control: A group treated with Comparative Example 1 (minoxidil, 'MIX') to the human dermal papilla cells after stress was induced in the dermal papilla cells by treatment with H ₂ O ₂

Experimental group: a group in which stress was induced in dermal papilla cells by treatment with H ₂ O ₂ and then the human dermal papilla cells were treated with Example 1 (germinated black rice extract)

After inoculating human dermal papilla cells into a 6-well plate, after 2 days, each well was scratched with a 100μL sterile tip, and the three groups except Control were treated with H ₂ O ₂ and then performed with Comparative Example 1 (Minoxidil, 'MIX') After treatment with Example 1 (germinated black rice extract), the scratched portion was photographed at 0 hr and 24 hr time points.

1 is an image confirming the degree of restoration of scratches artificially applied to dermal dermal papilla cells according to Example 1 (germinated black rice extract) and Comparative Example 1 (minoxidil, 'MIX') treatment.

As shown in FIG. 1, it was confirmed that most of the wounds were recovered 24 hours after scratching in both the 50 nM treatment group of Comparative Example 1 (minoxidil, 'MIX') and the 10 ppm treatment group of Example 1 (germinated black rice extract). It was confirmed that the black rice extract could promote the proliferation and differentiation of dermal dermal papilla cells.

<Experimental Example 2> Evaluation of Alkaline Phosphatase Expression Promotion Efficacy in Dermal Dermal Papilla Cells

The experimental group and the control group were configured in the same manner as in Experimental Example 1.

Specifically, the negative control, the positive control, and the experimental group except for the control induce stress in the dermal papilla cells by treating H ₂ O ₂ , and then the negative control is not treated with anything, and the positive control is Comparative Example 1 (minoxidil, 'MIX' ) and finally, the experimental group was treated with a fixing solution (4% paraformaldehyde) for 5 minutes 48 hr after the treatment with Example 1 (germinated black rice extract). The control was treated with a fixative solution without any treatment.

Then, after removing the fixing solution from each group, each group was treated with an alkaline phosphatase substrate, and after a certain period of time, the stained cells were washed with tertiary distilled water and stained blue under a microscope. cells were identified.

Figure 2 is an image confirming the expression level of Alkaline Phosphatase in dermal dermal papilla cells according to Example 1 (germinated black rice extract) and Comparative Example 1 (minoxidil, 'MIX') treatment.

As shown in Figure 2, it can be seen that the alkaline phosphatase expression level is significantly reduced in the hydrogen peroxide (H ₂ O ₂ ) treatment group compared to the normal control group (Control). Comparative Example 1 (minoxidil, 'MIX') 50nM treatment group and Example 1 (germinated black rice extract) 10ppm treatment group showed that the expression of Alkaline Phosphatase was restored to the normal control level. As confirmed, it was confirmed that the germinated black rice extract could promote proliferation and differentiation of dermal dermal papilla cells.

<Experimental Example 3> Comparison of hair growth efficacy of Example 1 (germinated black rice) and Comparative Example 2 (normal black rice)

Human hair follicle dermal papilla cells (HDPCs) were cultured in a low glucose DMEM medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin at 37 °C in a CO ₂ incubator.

In order to examine the hair growth efficacy through cell proliferation, the cell suspension was added to a 96-well plate at an appropriate concentration and then stabilized in an incubator for 24 hours. and 10 μg/mL) and cultured for 48 hours. After 48 hours, the media was removed and washed with DPBS.

After dispensing 100 μL of MTT solution dissolved in DPBS at a concentration of 1 μg/mL and incubating at 37 ° C for 4 hours, remove the supernatant and add 200 μL DMSO to completely dissolve formazan. OD values were measured in nm.

As a control group, a DMSO-treated group was used, and the hair growth rate (%) compared to the control group was measured and shown in Table 1 below.

Hair growth rate (%) Extract treatment concentration (μg/mL) One 2.5 5 10 Example 1
(germinated black rice) 108.84 120.57 123.45 125.28 Comparative Example 2
(regular black rice) 102.32 105.74 108.16 109.69

As shown in Table 1, in the case of Comparative Example 2 (general black rice) treatment group, the hair growth rate compared to the control group was insignificant, whereas in the case of Example 1 (germinated black rice) treatment group, it was confirmed that a significant effect on hair growth rate appeared. there was.

Production example of cosmetics

The active substance according to the present invention can be manufactured into various types of cosmetics depending on the purpose. The following exemplifies a method for preparing some cosmetics containing the active substance according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Cosmetics Production Example 1> Production of softened lotion

active substance 10.0 parts by weight

1,3-butylene glycol 1.00 parts by weight

Disodium EDT 0.05 parts by weight

allantoin 0.10 part by weight

dipotassium glycyrrhizate 0.05 parts by weight

citric acid 0.01 part by weight

sodium citrate 0.02 parts by weight

glycereth-26 1.00 parts by weight

Arbutin 2.00 parts by weight

Hydrogenated Castor Oil 1.00 parts by weight

ethanol 30.0 parts by weight

preservative a very small amount

coloring agent a very small amount

flavoring agent a very small amount

Purified water balance

<Cosmetic preparation example 2> Manufacture of nutrient cream

active substance 10.0 parts by weight

1,3-butylene glycol 7.00 parts by weight

glycerin 1.00 parts by weight

D-panthenol 0.10 part by weight

plant extract 3.20 parts by weight

Magnesium aluminum silicate 0.30 part by weight

PEG-40 Stearate 1.20 parts by weight

stearic acid 2.00 parts by weight

polysorbate 60 1.50 parts by weight

Lipophilic Glyceryl Stearate 2.00 parts by weight

Sorbitan sesquioleate 1.50 parts by weight

Cetearyl Alcohol 3.00 parts by weight

mineral oil 4.00 parts by weight

squalane 3.80 parts by weight

Carrylic/capric triglycerides 2.80 parts by weight

vegetable oil 1.80 parts by weight

dimethicone 0.40 parts by weight

Diposium glycyrrhizate a very small amount

allantoin a very small amount

sodium hyaluronate a very small amount

Tocopheryl Acetate Appropriate amount

triethanolamine Appropriate amount

preservative Appropriate amount

flavoring agent Appropriate amount

Purified water balance

Production example of health food

The active substance according to the present invention can be manufactured into various types of health food depending on the purpose. The following exemplifies methods for producing some health foods containing the active substances according to the present invention as active ingredients, but the present invention is not limited thereto.

<Health food manufacturing example 1> Manufacturing of dairy products

0.01-1 part by weight of the active substance of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<Health food manufacturing example 2> Manufacturing of food

Brown rice, barley, glutinous rice, and adlay were alphanized by a known method, dried, roasted, and then prepared into a powder having a particle size of 60 mesh using a grinder. Black beans, black sesame seeds, and perilla seeds were also steamed and dried by a known method, roasted, and then prepared into powder with a particle size of 60 mesh by a grinder. The active substance of the present invention was concentrated under reduced pressure in a vacuum concentrator to obtain a dry powder. It was prepared by blending the dry powder of grains, seeds and active substances prepared above in the following ratio.

Cereals (brown rice 34 parts by weight, adlay 19 parts by weight, barley 20 parts by weight),

Seeds (7 parts by weight of perilla seeds, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds),

Active substance (2 parts by weight),

reishi (1.5 parts by weight), and

Rehmannia glutinosa (1.5 parts by weight).

Production example of health functional food

The active substance according to the present invention can be manufactured into various types of health functional food depending on the purpose. The following exemplifies methods for producing some health functional foods containing the active substances according to the present invention as active ingredients, but the present invention is not limited thereto.

<Health functional food manufacturing example 1> Manufacturing of health functional food

active substance 100 mg

vitamin mixture Appropriate amount

Vitamin A Acetate 70 µg

vitamin E 1.0mg

vitamin B1 0.13mg

vitamin B2 0.15mg

vitamin B6 0.5mg

vitamin B12 0.2 µg

vitamin C 10 mg

biotin 10 µg

nicotinic acid amide 1.7mg

folic acid 50 µg

Calcium Pantothenate 0.5mg

mineral mixture Appropriate amount

ferrous sulfate 1.75mg

zinc oxide 0.82mg

magnesium carbonate 25.3mg

Potassium Phosphate Monobasic 15mg

Dibasic calcium phosphate 55mg

potassium citrate 90mg

calcium carbonate 100 mg

magnesium chloride 24.8 mg

Although the composition ratio of the above vitamin and mineral mixture was prepared by mixing ingredients suitable for relatively health functional foods in a preferred embodiment, the mixing ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health functional food manufacturing method. Then, granules can be prepared and used in the preparation of health functional food compositions according to conventional methods.

<Health functional food manufacturing example 2> Manufacturing of health functional beverages

active substance 100 mg

citric acid 100 mg

oligosaccharide 100 mg

plum concentrate 2 mg

taurine 100 mg

Add purified water to 500 mL

After mixing the above components according to the usual health drink manufacturing method, stirring and heating at 85 ° C. for about 1 hour, the resulting solution is filtered and obtained in a sterilized container, sealed and sterilized, and then refrigerated according to the present invention. It is used in the manufacture of health beverage compositions. Although the composition ratio is a mixture of ingredients suitable for a relatively favorite beverage in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and ethnic preferences such as the class of demand, the country of demand, and the purpose of use.

Manufacturing example of drug

The active substance according to the present invention can be formulated in various forms depending on the purpose. The following exemplifies some formulation methods containing the active substance according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Pharmaceutical Preparation Example 1> Preparation of powder

active substance 2g

lactose 1 g

After mixing the above ingredients, the powder was prepared by filling in an airtight bag.

<Pharmaceutical Preparation Example 2> Preparation of tablets

active substance 100 mg

corn starch 100 mg

Lactose 100 mg

magnesium stearate 2 mg

After mixing the above ingredients, tablets were prepared by tableting according to a conventional tablet manufacturing method.

<Pharmaceutical Preparation Example 3> Preparation of capsules

active substance 100 mg

corn starch 100 mg

Lactose 100 mg

magnesium stearate 2 mg

After mixing the above ingredients, capsules were prepared by filling gelatin capsules according to a conventional capsule preparation method.

<Pharmaceutical Preparation Example 4> Preparation of Injections

active substance 10 μg/mL

dilute hydrochloric acid BP until pH 3.5

Sodium Chloride BP for Injection up to 1 ml

The active substance according to the present invention was dissolved in an appropriate volume of sodium chloride BP for injection, the pH of the resulting solution was adjusted to pH 3.5 with dilute hydrochloric acid BP, the volume was adjusted with sodium chloride BP for injection, and mixed thoroughly. . The solution was filled into a 5 ml type I ampoule made of transparent glass, sealed under an upper grid of air by dissolving the glass, and sterilized by autoclaving at 120° C. for 15 minutes or longer to prepare an injection solution.

<Pharmaceutical Preparation Example 5> Preparation of Nasal Spray

active substance 1.0g

sodium acetate 0.3g

Methylparaben 0.1 g

Propylparaben 0.02g

sodium chloride Appropriate amount

HCl or NaOH pH Adjustment Appropriate amount

Purified water Appropriate amount

According to the conventional method for preparing nasal absorbents, saline (0.9% NaCl, w/v, solvent is purified water) is prepared to contain 3 mg of active substance per 1 mL, filled in an opaque spray container and sterilized to prepare nasal absorbents. manufactured.

<Pharmaceutical Preparation Example 6> Preparation of liquid formulation

active substance 100 mg

Lee Seonghwadang 10g

mannitol 5g

Purified water Appropriate amount

According to the conventional method for preparing liquids, each component is dissolved in purified water, lemon flavor is added, the above components are mixed, and purified water is added to adjust the total volume to 100 mL, and then filled in a brown bottle and sterilized to prepare a liquid formulation. did

Production example of topical skin preparation

The active substance according to the present invention can be formulated in various forms depending on the purpose. The following exemplifies some formulation methods containing the active substance according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Production Example 1 of external preparation for skin> Preparation of spray

active substance 100 mg

Lee Seonghwadang 10g

mannitol 5g

Purified water Appropriate amount

According to the conventional method for preparing a spray, add and dissolve each component in purified water, add lemon flavor, mix the above components, add purified water to adjust the total volume to 100 mL, fill in a brown bottle, and sterilize to prepare a spray. did

<External Skin Preparation Example 2> Preparation of gel

active substance 100 mg

ethanol 10g

glycerin 4g

propylene glycol 4g

Polyoxyethylene Hydrogenated Castor Oil 0.1 g

carboxypolymer 0.3g

triethanolamine 0.3g

antioxidant Appropriate amount

Spices Appropriate amount

antiseptic Appropriate amount

Purified water Appropriate amount

According to the conventional gel preparation method, each component was dissolved in purified water, flavor was added, and then the above components were mixed to prepare a gel formulation.

<External skin preparation preparation example 3> Preparation of ointment

active substance 100 mg

Cetostearyl Alcohol 2g

Self-emulsifying monostearic acid 2g

stearic acid 1 g

beeswax 4g

squalane 7g

monostearin glycerin 3g

Sorbitan Monostearate 1 g

Polysorbate 80 3g

glycerin 5g

propylene glycol 4g

Spices Appropriate amount

Vaseline balance

According to a conventional ointment preparation method, each component was added to Vaseline, dissolved, flavored, and then mixed with the above components to prepare an ointment.

So far, the present invention has been looked at with respect to its preferred embodiments. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a limiting point of view. The scope of the present invention is particularly indicated in the claims rather than the foregoing description, and all differences within the equivalent range should be construed as being included in the present invention.

Claims (8)

A cosmetic composition for preventing hair loss or promoting hair growth, comprising an extract of germinated black rice ( Oryza sativa L.).
According to claim 1,
The cosmetics are in the form of lotion, emulsion, cream, toner, lotion, serum, essence, emulsion, powder, cosmetic ointment, spray, gel, pack, cleanser, soap, shampoo, rinse, bath additive, detergent or conceal stick A cosmetic composition to do.
A health food composition for preventing hair loss or promoting hair growth, comprising an extract of germinated black rice.
According to claim 3,
The health food is a health food composition, characterized in that in the form of drinks, meat, sausages, bread, candy, snacks, noodles, ice cream, dairy products, soups, ionic beverages, beverages, alcoholic beverages, gum or tea.
A health functional food composition for preventing hair loss or promoting hair growth, comprising an extract of germinated black rice.
According to claim 5,
The health functional food is a health functional food composition, characterized in that in the form of tablets, capsules, pills or liquids.
A pharmaceutical composition for preventing or treating hair loss comprising an extract of germinated black rice.
According to claim 7,
The pharmaceutical composition is a pharmaceutical composition, characterized in that in the form of external preparation for skin.