KR101413132B1 - Sorhum with extremely high antioxidant activity - Google Patents

Abstract

The present invention relates to a new variety having high antioxidant activity characteristics, characterized in that the CS-4 local collection is cultivated through a purifying step.

Description

Antioxidant high activity {Sorgh with extremely high antioxidant activity}

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a method for improving the antioxidative activity by purifying water.

Sorghum (Sorghum bicolor L. Moench, sorghum) is a perennial herb that has been consumed mainly for edible purposes. Tropical Africa is the country's most cultivated land in the arid region and cultivated as grain sorghum, sorgo and broom-corn depending on the application, and in Asia, Africa and Central America It is a food resource being cultivated. Syrup is the world's fifth largest producer of rice, barley, wheat and corn in the world with a production of 57,772,000 tons. It contains a large amount of active ingredients such as dietary fiber and phenolic compounds. Phenolic compounds are composed of Condensed tannin, Flavonoid, Phenolic acid, and most of them are known as flavonoids. These phytochemicals are known to exhibit various health functional activities beneficial to the human body. The polyphenol extract of Acerola has been reported to have strong antimutagenic properties and is known to cause antioxidant activity in sorghum.

Antioxidation refers to the prevention of many oxidative effects in the body. Oxidation refers to the phenomenon that when various kinds of unsaturated fatty acids present in the body are oxidized by oxygen, a substance called lipid peroxidation is produced, and when it is increased, aging phenomenon is promoted. Human diseases and aging can be classified into O2- (superoxide), NO (nitric oxide), NO2 (nitrogen dioxide), OH (hydroxyl), ROO (proxyl), RO (alkoxyl) NO3 · (peroxynitrite) radicals. In vivo, superoxide dismutase (SOD), catalase (CAT), and catalase (CAT) have been used as means for protecting living organisms from free radicals, , Antioxidant enzymes such as glutathione peroxidase (GSHpx), vitamin E and glutathione are present to protect the living body from oxidative damage by eliminating free radicals. However, when such an antioxidant defense system declines to such an extent that free radical formation can not be controlled, the oxidative stress and damage of the tissue are promoted, and the excess free radicals and lipid peroxides produced in the body are oxidized by oxidative stress such as protein oxidation and DNA damage . Therefore, many synthetic or natural antioxidants have been developed for the reduction of oxidative stress. However, synthetic antioxidants have a problem in safety. In recent years, a safe and antioxidant natural antioxidant in various herbal and edible plant extracts We are making efforts to develop.

Although sorghum is the highest antioxidant activity of polyphenols and antioxidant components in grains, studies on the health function of sorghum have been carried out variously as described above. However, in order to effectively utilize the sorghum antioxidant activity in industry, antioxidant activity There is a need to develop this more prosperous aquatic plants.

The present invention provides a transplanting plant having more excellent antioxidant activity to effectively utilize the antioxidative activity of sorghum industrially.

The present invention relates to a novel product having an antioxidant activity activity characteristic, characterized in that the product is cultivated through a purifying step of CS-4 Local collection (Accession No. IT201458, Accession No. ACC 6, Depository: NPGRL) Lt; / RTI >

The sorghum may have antioxidant activity 1.5 to 2.5 times higher than the genetic source.

In addition, the present invention can provide a pharmaceutical composition for an antioxidant containing, as an active ingredient, an antioxidant highly active extract derived from a lower alcohol, preferably a C ₁ -C ₄ alcohol.

In addition, the present invention can provide a health functional food containing, as an active ingredient, an antioxidant highly active hydrolyzate extract obtained by extracting the marinade with a lower alcohol.

&Quot; Milyang No. 3 " of the present invention means a new variety having an antioxidant activity activity raised according to the present invention.

The present invention can provide a new variety having improved antioxidant activity to utilize the antioxidative activity of sorghum in an industrial aspect.

Fig. 1 is a pedagogical genealogy chart which invented a new variety "Milyang No. 3" through a series of pure segregation breeding of CS-4 local collection.
Fig. 2 shows the shape of the ears of the new variety "Milyang No. 3".

The present invention relates to a new variety having a high antioxidant activity characteristic, characterized in that the CS-4 local collection introduced into the genetic circle center (accession number: IT201458, accession number: ACC 6, donor: NPGRL) Milyang No. 3 'seeds, its seeds and the seeds of the seeds.

The seeds of the new variety "Milyang No. 3" of the present invention are to be understood as including seeds of millet or millet, including their offspring.

The purple segregation process is a breeding method used for a variety of plants, using a modification method in which one of the varieties of mixed organisms is subjected to self-fertilization or culling to select pure lines.

The antimicrobial activity of the new varieties 'Milyang 3' can be 1.5 to 2.5 times higher than the 76 points of genetic resources.

In addition, the new variety 'Milyang No. 3' may contain at least one component selected from the group consisting of polyphenols, tannins, and proanthocyanidins, which are antioxidant components, in an amount of 1.2 to 1.5 times as much as the gold streak.

Polyphenols are one of the antioxidants that turn our body's active oxygen (harmful oxygen) into harmless substances, and there are thousands more. Among them, catechins in green tea, resveratrol in wine, apple and quercetin in onion are relatively well known. Many flavonoids in fruits and many isoflavones in beans are also a kind of polyphenols. Polyphenols have been reported to reduce the risk of various diseases because of their high antioxidant ability to protect cellular DNA and enzymes as well as to protect DNA damaged by exposure to reactive oxygen species. Polyphenols are also known to block heart disease with anticancer effects.

Tannin is widely distributed in many plants. Aqueous solution is a general term for compounds with strong astringency and pungent taste. The composition and content of tannin contained in fruits and vegetables vary according to kinds and cultivars. Tannin is known to be good for the prevention and treatment of adult diseases such as hypertension, hyperlipemia, and stroke because the polyphenol component which is contained in the tannins dissolves the fat to prevent fat accumulation. It is known that the action of releasing the nicotine component from the body and removing the harmful oxygen And is known to have an antioxidant effect.

Proanthocyanidins are known to have antioxidative effects of vitamin C 20 times and vitamin E 50 times. They are known to prevent blood deposits in the arteries, clear blood, and reduce the risk of heart disease and stroke. In addition, it is known that the effect of protecting skin cells from external harmful environments is also excellent.

Flavonoids are also known as vitamin P (permeable vitamins) or vitamin C ₂ (a vitamin C uptake). It is relatively small in animals, and contains many of the leaves, flowers, roots, fruits, and stems of plants. It has antibacterial, anticancer, antiviral, antiallergic and anti-inflammatory activity, and it is reported that there is little toxicity. In addition, as the fact that it inhibits in vivo oxidative action is known, there is a growing interest in the development and application of flavonoid-based materials.

The present invention provides an antioxidant highly active extract obtained by extracting the above-mentioned new variety 'Milyang No. 3' with low alcohol, preferably C ₁ -C ₄ alcohol.

In addition, the present invention provides a pharmaceutical composition for an antioxidant containing the above-mentioned antioxidant highly active hydrolyzate as an active ingredient.

The pharmaceutical composition for the antioxidant is a substance that releases electrons to active oxygen to prevent oxidation of the gene by self-oxidation. The active oxygen is reduced by an antioxidant which is a substance that removes active oxygen, thereby losing carcinogenicity. Therefore, antioxidants are cancer-preventing substances and chemically reducing agents. That is, an antioxidant is a substance that protects the body from oxidation by active oxygen by removing active oxygen.

The pharmaceutical compositions comprising the antioxidant highly active hydrolyzate extract according to the present invention as an active ingredient may be administered orally or parenterally in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups or aerosols, Suppositories, or sterile injectable solutions.

More specifically, when formulating the composition, it can be prepared using a diluent or an excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, and the like. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, Sucrose, lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Examples of liquid formulations for oral use include suspensions, solutions, emulsions and syrups. In addition to water and liquid paraffin which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

The amount of the antioxidant highly active extract according to the present invention may vary depending on the age, sex and body weight of the patient, but is generally in the range of 0.01 to 500 mg / kg, preferably 0.1 to 100 mg / Can be administered separately. The dose of the antioxidant highly active extract can be increased or decreased depending on the route of administration, disease severity, sex, weight, age, and the like. Accordingly, the dosage is not limited in any way to the scope of the present invention.

In addition, the present invention can provide a health functional food containing an antioxidant highly active water extract, which is an alcohol extract of the above-mentioned new variety 'Milyang No. 3'.

There is no particular limitation on the kind of health functional food. Examples of foods to which the above antioxidant highly active extracts can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, Tea, a drink, an alcoholic beverage, and a vitamin complex, and can be used in the form of pills, powders, granules, infusions, tablets, capsules or beverages, and includes health functional foods in a conventional sense.

In this case, the amount of the antioxidant highly active extract in the food or drink may be generally 0.01 to 15% by weight of the total food weight of the health functional food composition of the present invention, and 0.02 to 100% To 10 g, preferably from 0.3 to 1 g.

The health beverage composition of the present invention is not particularly limited as long as it contains the above-mentioned antioxidant highly active hydrolyzate as an essential ingredient in the indicated ratio, and includes various flavors or natural carbohydrates as an additional ingredient . Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; Polysaccharides such as dextrin, cyclodextrins; And sugar alcohols such as xylitol, sorbitol, and erythritol. As natural flavors other than those described above, natural flavors (such as tau martin, stevia extract (e.g., rebaudioside A, glycyrrhizin)) and synthetic flavors (saccharin, aspartame, etc.) have.

In addition to the above, the health functional food of the present invention may contain various kinds of nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and heavies (cheese, chocolate etc.), pectic acid and its salts, And salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like.

In addition, the health functional foods of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination.

In addition, the new variety of the present invention 'Milyang No. 3' can be provided as materials for food processing such as mixed rice, baking, noodles, confectionery and drinks.

According to a preferred embodiment of the present invention, the material for food processing of the new variety can be used for baking and noodles.

Hereinafter, the present invention will be described in detail with reference to examples. However, these examples are intended to further illustrate the present invention, and the scope of the present invention is not limited to these examples.

[ Example ]

Example  One: Purple seperation .

20 individuals of the CS-4 Local collection (accession number: IT201458, accession number: ACC 6, donator: NPGRL) introduced into the genetic resource center in 2009 were deployed at the National Institute of Food Science and Technology, Rural Development Administration. It is known that the fertilization rate is 1 ~ 10%, but it is 30 ~ 60% depending on the density of the ears. Therefore, to increase the purity of the reared object, it is necessary to put an envelope on the ear of each individual before flowering, Respectively. Among them, 3 lines with excellent agricultural traits were selected. In 2010, 20 lines were planted in each line. We selected the CS-4 Local collection-2 system, which has uniform agricultural characteristics, high antioxidant activity, and strong resistance to the clothing, according to the 2009 Purity Separation Method, and gave the system name to Milyang No. 3 in 2011. Two-year productivity test and regional adaptation test were carried out in Zhongshan Gangjia (Wonju) and Inland Plain (Milyang) in the north and central part, and the characteristics such as yield and antioxidant activity were tested (Fig. 1).

Example  2: With gold rattles  New variety ' Milyang 3 'Comparison of morphological features of sorghum.

The new varieties 'Milyang No. 3' are of the ovate morphology of the ears, the dense density of the ears, the dark color of the berries and the color of the seeds are dark brown, the endosperm is mature and the juice of the stem is sweet sorghum containing sugars See Table 1). The length of the ears is 26cm, the width of the ears is 7cm, and the weight of the ginseng is 25.0g, which is somewhat heavier than the 'golden chrysanthemum' (see Table 2). In addition, the shape of the ear of a new variety "Milyang No. 3" is attached to FIG.

Breed name ear Bell Seed color Drainage characteristics
(Amylose%) Sugar content
(Brix °%) shape density Milyang 3 Ovary Smuggling type Light brown Light brown MeSung (28.6%) 12.1 A gold rally Ovary Smuggling type maroon maroon Stickiness (9.9%) -

Breed name Heading
(Mon. Sun.) key
(cm) Spindle length (cm) Leaf
(cm) Leaf width
(cm) Isaac Zhang
(cm) Isaac width
(cm) Grain weight (g / pieces) Heavenly
(g) Milyang 3 08.03 151 11.3 85.3 6.9 26 7 25.1 25.0 A gold rally 07.26 159 18.5 61.1 9.1 27 8 23.1 21.2

Example  3: New variety ' Milyang 3 Manufacture of extracts of sorghum.

For the preparation of the extracts for the antioxidant activity, 'Milyang No. 3' (seedlings) seeds were used and pulverized with a vibrating sample mill (CMT Co. Ltd., Tokyo, Japan) immediately before analysis. 10 g of the milled millet was taken and 100 ml of 80% methanol was added. The mixture was subjected to shaking extraction (SK-71 Shaker, JEIO Tech, Kimpo, Korea) for 3 hours at room temperature for 24 hours, followed by filtration. Tokyo, Japan) at 40 < [deg.] ≫ C. After reconstitution with 80% methanol, 100 ㎖ of the solution was used for storage at -20 ℃ in the freezer. The extracts were diluted to a concentration of 10 ㎎ / ㎖.

Experimental Example  1: New variety ' Milyang 3 'Antioxidant Activity and Antioxidant Activity of.

The antioxidant activities of the extracts of the new variety "Milyang No. 3" extracted in Example 3 were DPPH (1,1-diphenyl-2-picryl-hydrazyl, Sigma-Aldrich) and ABTS (2,2'-azino- -ethylbenzo-thiazoline-6-sulfonic acid, Sigma-Aldrich), and the scavenging activity of DPPH and ABTS radicals was expressed as mg TE (Trolox equivalent antioxidant capacity) / g sample (sample, dry basis) .

The scavenging activity of the DPPH radical was determined by adding 0.2 ml of a 0.2 mM DPPH solution (dissolved in 99.9% ethanol) to 0.8 ml of the DPPH solution, and then measuring the absorbance at 520 nm for 30 minutes. The ABTS radical scavenging activity was determined by incubating 7.4 mM ABTS and 2.6 mM potassium persulphate in a dark place overnight to form ABTS cations. The ABTS cations were then incubated at 735 nm with a molar extinction coefficient ε = 3.6 × 10 ⁴ M ^-1 * cm ^-1 ). To 1 ml of the diluted ABTS solution was added 50 쨉 l of the extract of the new variety "Milyang No. 3" extracted in Example 3, and the change in absorbance was measured 30 minutes later.

The DPPH radical scavenging activity and the ABTS radical scavenging activity were measured in the same manner as in the method of the present invention. The measured DPPH and ABST radical scavenging activities of the new variety "Milyang No. 3", 73 genetic resources and gold pollens were shown in Table 3 below.

Resource name Radical scavenging activity (mg TE / g) DPPH ABST New variety 'Milyang No. 3' 22.1 24.8 Average of 73 genetic resources 7.0 (0.02 to 22.1) 11.5 (5.2-24.8) A gold rally 11.9 13.4

As shown in Table 3, it can be seen that the DPPH and ABST radical scavenging activities of the new variety "Milyang No. 3" are higher than the average of the 73 genetic pollen sources of the present invention and the gold pollution number of the standard variety. Therefore, it was confirmed that the new variety "Milyang No. 3" has an antioxidative activity superior to that of the present invention.

The antioxidative activities of the new variety "Milyang 3" and the gold leaf, which is a standard variety, were measured by the same method as the ABTS radical scavenging activity measurement. The measured ABST radical scavenging activity is shown in Table 4 below.

Cultivation by Region Cultivation year discrimination ABTS radical scavenging activity (mg TE / g) New variety 'Milyang No. 3' A gold rally Won-ju 2011 49.5 36.5 Miryang 2011 35.2 28.6 Miryang 2010 30.5 21.1 Average - 38.4 28.7

As shown in Table 4, it was confirmed that the antioxidant activity of the new variety "Milyang 3" was superior to the antioxidant activity of the gold leaf, which is a standard variety, irrespective of the cultivation area and cultivation year.

Experimental Example  2: New variety ' Milyang 3 'Measurement and Comparison of Antioxidant Contents in Acer.

The total polyphenol content of the water extract of the new variety "Milyang No. 3" extracted in Example 3 was such that the Folin-Ciocalteu phenol reagent was reduced in molybdenum blue as a result of the reduction by the polyphenolic compound of the water extract . Add 50 μl of 50% Folin-Ciocalteu reagent (Sigma-Aldrich, St. Louis, Mo., USA) to 50 μl of the extract, add 1 ml of 2% Na ₂ CO ₃ solution and allow to stand for 3 minutes. . After 30 minutes, the absorbance of the reaction solution was measured at 750 nm, and a calibration curve was prepared using gallic acid (Sigma-Aldrich) as a standard. The glycine equivalent (GAE, dry basis.

The total flavonoid content was determined by adding 1 ml of distilled water and 75 μl of 5% NaNO ₂ to 250 μl of the extract, adding 150 μl of 10% AlCl ₃₆ H ₂ O for 5 minutes, allowing to stand for 6 minutes, adding 500 μl of 1 N NaOH, . After 11 minutes, the absorbance value of the mixed solution was measured at 510 nm. Calibration curves were prepared using the standard material (+) - catechin ((+) - catechin, Sigma-Aldrich) and expressed as mg catechin equivalent (CE, dry basis) in 1 g of the extract.

The total tannin content was determined by adding 1 ml of 95% ethanol and 1 ml of distilled water to 1 ml of the new variety "Milyang No. 3" extracted from Example 3, shaking it well, adding 1 ml of 5% Na ₂ CO ₃ solution, 0.5 ml of a reagent (Folin-ciocalteu reagent, Sigma-Aldrich) was added, and the mixture was developed at room temperature for 60 minutes. Then, the absorbance was measured at 725 nm and a calibration curve was prepared using tannic acid (Sigma-Aldrich) Mg tannic acid equivalent (TAE, dry basis) in 1 g of the extract.

The content of proanthocyanidin was determined by adding 1 ml of methanol to 100 mg of the crushed wheat flour in Example 3, stirring the mixture, adding 2 ml of 1% (w / v) vanillin / methanol and stirring. 2 ml of 25% (v / v) sulfuric acid / methanol was added and stirred, followed by shaking at 30 ° C for 15 minutes, adding 1 ml of methanol and stirring. After centrifugation at 4,000 rpm for 10 minutes, the supernatant was taken at 500 nm, and the calibration curve was drawn with (+) - catechin (Sigma-Aldrich) as a standard. Mg catechin (catechin, dry basis).

The antioxidative activity components of the golden wax were measured by the same method as in the measurement of the content of polyphenol, tannin, flavonoid and proanthocyanidin used above.

The antioxidative activity components of the new variety, 'Milyang No. 3', and the gold picket fungus, as measured above, are shown in Table 5 below.

System name Polyphenol content
(mg / g) Tannin content
(mg / g) Proanthocyanidin
Content (mg / g) Flavonoid
Content (mg / g) New variety 'Milyang No. 3' 27.1 7.3 0.34 5.69 A gold rally 13.0 2.3 0.25 5.73

As shown in Table 5, it was confirmed that the antioxidant active ingredient content of the new variety "Milyang No. 3" was significantly higher than that of gold. Especially, the content of polyphenol was 2.1 times higher than that of gold leaf in the new variety "Milyang 3".

As can be seen from the foregoing Examples and Experimental Examples, it can be confirmed that the new variety "Milyang No. 3" according to the present invention has an antioxidative activity-enhanced property than the standard variety, golden pollens and 73 points of genetic resources. In addition, it was confirmed that the content of polyphenol, tannin and proanthocyanidin in the extract of the new variety "Milyang No. 3" of the present invention was much higher than that of the gold leaf as a standard variety.

[ Manufacturing example ]

Manufacturing example  1: Preparation of pharmaceutical composition

The present invention will now be described in detail with reference to the following examples, but it should be understood that the present invention is not limited to these examples.

The extract of 'Milyang No. 3', which was used in the following production example, was the extract of Milyang No. 3 manufactured in Example 3.

Manufacturing example  1-1. Sanje  Produce

New variety 'Milyang No. 3' water extract 2 g

Lactose 1 g

The above components were mixed and packed in airtight bags to prepare powders.

Manufacturing example  1-2. Manufacture of tablets

New variety 'Milyang No. 3' 100 mg extract

Corn starch 100 mg

100 mg of milk

Magnesium stearate 2 mg

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

Manufacturing example  1-3. Preparation of capsules

New variety 'Milyang No. 3' 100 mg extract

Corn starch 100 mg

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

Manufacturing example  1-4. Manufacture of rings

New variety 'Milyang No. 3' water extract 1 g

Lactose 1.5 g

Glycerin 1 g

0.5 g of xylitol

After mixing the above components, they were prepared so as to be 4 g per one ring according to a conventional method.

Manufacturing example  2: Preparation of health functional foods.

The present invention will now be described in detail with reference to the following examples, but it should be understood that the present invention is not limited thereto.

The extract of 'Milyang No. 3', which was used in the following production example, was the extract of Milyang No. 3 manufactured in Example 3.

Manufacturing example  2-1. Manufacture of Health Functional Foods

New varieties 'Milyang No. 3' extracts 3000 mg

70 [mu] g of vitamin A acetate

Vitamin E 1.0 mg

0.13 mg vitamin B1

0.15 mg of vitamin B2

10 mg vitamin C

Biotin 10 μg

50 ㎍ of folic acid

Calcium pantothenate 0.5 mg

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

15 mg of potassium phosphate monobasic

Potassium citrate 90 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is relatively mixed with a suitable preparation for health food, the compounding ratio may be arbitrarily changed, and the above ingredients may be mixed according to a conventional method for producing healthy food , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Manufacturing example  2-2. Manufacture of health drinks

New variety 'Milyang No. 3' extracts 3 g

Citric acid 1000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added to a total of 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated for about 1 hour at 85 DEG C with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, Of health functional beverage composition.

Although the composition ratio is relatively mixed with the ingredients suitable for the favorite beverage as a preferable preparation example, the blending ratio may be arbitrarily modified according to the local or national preference such as the demand class, demand country, use purpose, and the like.

Manufacturing example  3: Manufacturing of food.

The present invention will now be described in detail with reference to the following examples, but it should be understood that the present invention is not limited thereto.

The new variety produced according to the present invention is crushed by the mill after the marching to the water. The millet mill powder of the new variety "Milyang No. 3" used in the following production example was used.

Manufacturing example  3-1. Manufacture of noodles

New varieties 'Milyang No. 3' sorghum powder 50 parts by weight

100 parts by weight of wheat flour

Water 40 parts by weight

5 parts by weight of salt

The noodles were prepared by kneading the above-mentioned contents of wheat flour, water, salt and new varietal millet for 3 minutes and then aging at 30 ° C for 1 hour in a plastic bag. Then, after the rollers were formed at a roll interval of 4 mm, the rollers were combined again to form a 4 mm sheet, and the thickness of the sheet was gradually reduced to 3, 2 and 1.5 mm through the rolls of three stages. Finally, 1.5 ㎜ and 1.5 ㎜ width were prepared.

Manufacturing example  3-2. Manufacture of bread

New variety "Milyang No. 3" sorghum powder 40 weight parts

200 parts by weight of wheat flour

Water 250 parts by weight

East 6 parts by weight

15 parts by weight of sugar

5 parts by weight of salt

Shortening 4 parts by weight

10 parts by weight of skim milk powder

The flour dough was prepared by mixing the above-mentioned contents of wheat flour, water, new kinds of flour, yeast, sugar, salt, shortening, and skim milk powder. To 200 parts by weight of the flour used in the dough, 40 parts by weight of a new variety of flour And the dough was directly kneaded by a kneading method. The mixture was first fermented at 28 ° C and 75% relative humidity for 60 minutes and then fermented at 28 ° C and 75% relative humidity for 20 minutes and baked in a 160 ° C oven for 20 minutes.

The composition ratio of the bread or the noodle composition is comparatively comparable to that of a preferable preparation example. However, the blending ratio may be arbitrarily varied according to the regional or national preference such as the demand level, the demanding country, and the use purpose.

Agricultural Biotechnology Research Institute KACC98013 20121015

Claims (11)

Characterized in that it comprises at least one component selected from the group consisting of polyphenols, tannins and proanthocyanidins, which are antioxidant components, in an amount 1.2 to 1.5 times higher than that of gold, and has the following horticultural properties: Sorghum bicolor L.) Moench) Milyang 3 (Deposition: Institute of Agricultural Biotechnology, Accession No .: KACC98013P, deposit date: Oct. 15, 2012).
1) Egg shape: oval
2) Density of thread: Smooth
3) Seed color: Light brown
4) Seed coat color: light brown
5) Outflow characteristics: delete delete Seeds of Sorghum bicolor (L.) Moench Milyang No. 3 (Depository Institution: National Institute of Agricultural Biotechnology, Accession No .: KACC98013P, deposit date: Oct. 15, 2012) The offspring of the seeds of the seeds of paragraph 4. An antioxidant highly active citrus extract obtained by extracting one or more selected from the group consisting of C ₁ -C ₄ alcohols, delete delete delete Characterized in that it comprises the Sorghum bicolor (L.) Moench Milyang 3 (Depository Institution: National Institute of Agricultural Biotechnology, Accession No .: KACC98013P, deposit date: Oct. 15, 2012) Characterized in that it comprises the Sorghum bicolor (L.) Moench Milyang No. 3 (Depository Institution: National Institute of Agricultural Biotechnology, Accession No .: KACC98013P, deposit date: Oct. 15, 2012)

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