KR101426232B1 - Astringent cosmetic composition containing extract of rice cultivated with Phellinus baumii mycelium as effective component - Google Patents

Abstract

The present invention relates to a cosmetic composition and a functional food containing an extract of Phellinus linteus mycelium cultured as an active ingredient and more particularly to a cosmetic composition and a functional food containing a pancreatic mycelial culture extract, And the like. The present invention also relates to a cosmetic composition or a functional food.

Description

[0001] The present invention relates to a cosmetic composition containing an extract of Phellinus baumii mycelium as an active ingredient,

The present invention relates to a cosmetic composition and a functional food containing an extract of Phellinus linteus mycelium cultured as an active ingredient and more particularly to a cosmetic composition and a cosmetic composition, Functional food.

Until the early 1990s, simple foods such as bread, ramen, and other flour remained an explosive popularity among modern people. At that time, the food was quick to eat, and there was a lot of interest in the preference of the food, so the preference of flour was high. With the rapid economic growth in the 1990s, consumers' interest in slow food and our own food has been increasing steadily as the living standards of consumers have improved, the life expectancy has been extended due to the development of modern medicine, and the aging population has increased Trend. Accordingly, there is a growing interest in products that have higher nutritional, physiologically active, and useful ingredient contents than flour and processed rice with less digestive disorders.

Domestic rice production has increased significantly since 2000, when the supply of rice has increased compared to demand, and surplus resources have been generated. Recently, the consumption of rice, which is overproduced, is not enough, and the government is trying to prepare measures. Among the products using rice, rice with a high consumption of rice is rice for eating rice and rice for rice, rice for consumption is difficult to stimulate consumption of rice due to the influence of hatched familyization, rice for rice is used for rice wine, It has the disadvantage that it is below the expectations of consumers. Therefore, there is a growing interest in the development of functional products fermented with useful microorganisms for rice, and the expectation for improvement of rice digestibility, functional improvement and product diversification due to the action of various microorganisms is increasing. In recent years, there has been a growing interest in health due to the well-being craze, and the demand for functional foods has been increasing for the prevention of chronic diseases, and thus the development of functional foods has become a major task of the modern food industry. Therefore, development of health - oriented products using natural materials is progressing, and studies on the functionality of foods are actively carried out.

Black rice ( Oryza sative , Black Rice) is rice with pigment of magenta in rice bran layer. Its main producing area is China or Southeast Asia, and Korea is also produced in some areas such as Jindo, Haenam and Boseong. In addition, black rice is rich in nutrients such as protein, vegetable fat, fiber, minerals and vitamins, and is known to have high antioxidant and antimutagenic effects. Antioxidants include cyanidin 3-O-β-glucoside, peonidin3-O -β-o-glucoside have been reported. Anthocyanins, commonly found in red and black fruits and vegetables, are known to be highly antioxidant.

Oryza sative (Red Rice) is a rice similar to wild rice species produced in West Asia region. It has a reddish brown pigment. These red rice ingredients are relatively stable even in heat and can be safely used even in acidic conditions. Cyanidin- 3-glucoside. In addition, red rice contains a large amount of anthocyan pigments, and pigments with anthocyan pigments have been reported to have a stronger antioxidative effect than pigments with nonanthocyan pigments. Particularly, rice bran contains a large amount of catechol tannin called anthocyanin and catechin, and the antioxidative action by this is attracting attention. This is because various diseases caused by oxidative stress are known to be prevented by antioxidant activities of catechin, vitamin E, superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-Px) In particular, it is well known that antioxidant enzymes and antioxidant vitamins improve lipid metabolism in relation to lipid metabolism.

About 20,000 species of mushrooms are known worldwide, of which about 2,000 species can be developed for food. Approximately 992 species of mushrooms distributed in Korea are recorded. Among them, about 100 kinds of edible mushrooms and 50 kinds of mushrooms are found. Especially, more than 20 kinds of mushrooms having high toxicity are identified. More than 20 kinds of mushrooms can be cultivated in Korea, and have been proven to have anti-cancer, low cholesterol and low blood sugar. There are about 280 kinds of mushrooms around the world. Most of the mushrooms grow in yellow and form a ring pattern, which is one of the most widely artificially grown mushroom cultivars. A typical example of the situation mushroom is woody mud mushroom Phellinus It is known as linteus . However, in Korea, a representative cultivar is Bormi situation mushroom ( Phellinus baumii ) accounts for more than 98% of the total dismantled farm households. Unusually, it is known that Korea is the only place where it is allowed to be edible and used as a health promotion food. So far, various studies on the separation of physiologically active substances from mushrooms have been continuously conducted, Changes in the efficacy and evaluation of mushroom extracts are under review. Various mycelial activities such as blood anticoagulant activity, antioxidant activity, anticancer activity, gastric ulcer relaxation effect and anti-inflammatory activity similar to those of mushroom fruiting body and β-secretase inhibitory activity for treating Alzheimer disease have been reported in mycelium in addition to fruiting bodies Much research has been focused on the mycelial culture of mushrooms. In order to reduce skin irritation and to enhance functional ingredients, the present invention uses functional pigments such as Phellinus baumii (situation mushroom) hypha is cultured to utilize as functional food and natural cosmetics raw material.

Korean Patent Registration No. 0946584 discloses a cosmetic composition for astringent and skin irritation alleviation containing a root extract and a blue lotus extract, and Korean Patent No. 0829720 discloses a astringent cosmetic composition containing an extract of bokbunja as an active ingredient However, it differs from the astringent cosmetic composition containing the extract of the mushroom cultured mycelium of the present invention.

The present invention has been made in view of the above needs, and it is an object of the present invention to provide a cosmetic composition and a functional food containing the extract of a mushroom mycelium cultivar which is remarkably excellent in astringent activity compared with a general or coloring matter extract, Respectively.

In order to solve the above problems, the present invention Phellinus (Phellinus The present invention provides a cosmetic composition having a skin-converging action containing, as an active ingredient, an extract of a mycelium of Mycobacterium tuberculosis .

In addition, the present invention relates to a method for producing a mushroom The present invention provides a functional food having a skin-converging action containing an extract of Mycelium mycelium as an active ingredient.

According to the present invention, there is provided a cosmetic composition which is excellent in its effect because it contains a physiologically active substance obtained from plants and has remarkably excellent convergence activity effect, and which does not cause side effects .

In addition, the method of the present invention extracts cultured mushroom mycelia to maximize the content of the active ingredient, remove impurities to improve the stability, and maximize the efficacy of the extract to a skin external preparation, for example, , Cream, essence, etc., as well as functional foods for skin astringency.

Figure 1 shows the mycelia mycelium (JMI-P2), mycelium culture mycelium culture (RRCP) and cultured mycelium culture broth (BRCP).
FIG. 2 is a graph showing the survival rate of the melanoma cells (B16F10) according to the concentration of rice extract and rice bran cultured mycelium extract.
red: Red mulberry extract, RRCP: Culture of mycelium of mycelia of mushroom, black: Black rice extract, BRCP: Mycelium of mushroom culture black rice extract
FIG. 3 is a graph showing the survival rate of fibroblast cells according to the concentration of rice extract and rice bran cultured mycelium extract. FIG.
red: Red mulberry extract, RRCP: Culture of mycelium of mycelia of mushroom, black: Black rice extract, BRCP: Mycelium of mushroom culture black rice extract
FIG. 4 is a graph comparing the convergent activities of the extracts of the rice extract and the mushroom mycelium cultured according to their concentrations. FIG.
Red rice: Rip roots extract, RRCP: Culture of mycelia of mycelia, Black rice: Black rice extract, BRCP: Black mushroom cultivation mycelium culture

According to an aspect of the invention, the invention is Phellinus (Phellinus The present invention provides a cosmetic composition having a skin-converging action containing, as an active ingredient, an extract of a mycelium of Mycobacterium tuberculosis .

In the composition of the present invention, the above-mentioned mushroom ( Phellinus baumii ) Mycelium extract The situation on the grain sterilized mushroom (Phellinus The mycelia of the mycelium cultured at 28 to 32 ° C after inoculation with mycelium of mycelia can be lyophilized, pulverized, extracted with ethanol, and concentrated under reduced pressure at 45 to 55 ° C. The sterilized cereal was fed with a mushroom ( Phellinus The present invention also relates to a method for producing a mycelium of the present invention, which comprises culturing the mycelia of the mycelia of the present invention cultured at 30 ° C after inoculation of the mycelium with the mycelium of the present invention, lyophilization, pulverization, ethanol extraction and concentration at a reduced pressure at 50 ° C.

The cosmetic composition containing the mushroom mycelium culture extract according to the present invention preferably contains 0.0001 to 10% by weight of the mushroom mycelium culture extract, based on the total weight of the composition, but is not limited thereto.

The mycorrhizae mycelium cultivated extract of the present invention is characterized by having a converging effect, and the rice mushroom mycelium extract has higher convergence activity than other rice extracts. Therefore, it is characterized in that it has remarkably high skin converging effect as compared with the cosmetic composition using the conventional rice extract.

Also, in the composition of the present invention, the cosmetic composition can be used as a cosmetic material for cosmetics such as a softener, a cosmetic lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, an eye essence, a cleansing cream, a cleansing foam, a cleansing water, But are not limited to, formulations selected from the group consisting of body creams, body oils, and body essences.

The ingredients contained in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the extract of the mushroom mycelium extract as an active ingredient and can be prepared by conventional methods such as antioxidants, stabilizers, solubilizers, vitamins, Supplements, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a flexible lotion (skin), a nutritional lotion (milk lotion), a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

The invention also Phellinus (Phellinus The present invention provides a functional food having a skin-converging action containing an extract of Mycelium mycelium as an active ingredient.

In the case of using the cultured mycelium extract of the present invention as a food additive, the extract may be used as it is, or may be used together with other food or food ingredients, and suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the extract of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, in the production of food or beverage. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of the food to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which contain functional foods in a conventional sense.

The functional beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient, such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the extract of Mycelia mycelium cultured according to the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, Glycerin, alcohols, carbonating agents used in carbonated beverages, and the like. In addition, the extract of Mycelium mycelium cultured according to the present invention may contain flesh for the production of natural fruit juice, fruit juice drink and vegetable drink. These components may be used independently or in combination. Although the ratio of such additives is not critical, the extract of the present invention is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Materials and methods

1. Experimental material

In this experiment, Phellinus baumii was used in Jungheung mushroom industry research institute and Oryza sativa (Black Rice) and red rice ( Oryza sativa , Red Rice) were purchased from the Gwangju area.

Each of the black rice and red rice was washed with water and then water soaked for 4 hours. After removing water for 30 minutes, 600 g of each grain was put into the culture bag and sterilized at 121 ° C for 15 minutes. After freezing the sterilized grain, the situation is being preserved by Jangheung-gun Mushroom Industry Research Institute ( Phellinus The mycelium was inoculated on the surface of the grains and incubated at a temperature of 30 ° C. For the safety and efficacy screening, each of the ground samples was diluted with 25 mL of 80% ethanol (250 mL) and concentrated under reduced pressure at 50 ° C. After diluting with about 25 mL, it was lyophilized and used.

2. Fatty acid analysis

The fatty acids of the samples were analyzed by GC-MS (Agilent 7890A, Palo Alto, Calif., USA). The analytical conditions were the Agilent 5975C MSD (Agilent, Palo Alto, CA, USA) WAX column (250 mm L x 0.25 mm ID, manufactured by Agilent Co., USA), and carrier gas was helium. Injection volume was 1.0 mL / min, and 1 g of the sample was added to 100 mL of ether and refluxed with a hot plate at 100 ° C. The extracts were filtered, evaporated to dryness in a constant temperature water bath at 60 ° C, and then 2 mL of 0.5 N methanolic sodium hydroxide was added and refluxed for 5 minutes. After extraction, 2 mL of 14% BF3-methanol was added and the mixture was refluxed for 30 minutes. 4 mL of n-hexane was added to the above solution, and the mixture was refluxed for 2 minutes. After saturated sodium chloride was added, the upper layer was separated and filtered through a 0.45 ㎛ membrane filter. The retention time of each peak was determined using EC10A-kit (Supelco, USA) as a standard form and identified using the spectra of the GC-MS Wiley library. The injector temperature was maintained at 250 ° C and the column temperature was maintained at 140 ° C for 2 minutes. The temperature was increased to 200 ° C at 5 ° C per minute, and then increased to 10 ° C per minute at 250 ° C. The hold time was initially set to 10 minutes from 50 to 250 ° C, and adjusted to 4 minutes at 250 ° C.

3. Safety and validity search

end. Safety experiments in vitro

1) Cell line and cell culture method

The melanoma cells used in this study were B16F10 and fibroblast cells were purchased from Korean cell lines bank. Cells were cultured in DMEM (Dulbecco's Modified Eagle Medium) medium containing 10% FBS and antibiotic-antimycotic and incubated at 37 ° C in a 5% CO ₂ incubator. When cells were collected, 1 mL of trypsin-EDTA was added, and the cells were separated by incubating at 37 ° C for 5 minutes. 4 mL of the medium was added and centrifuged at 1,200 rpm for 3 minutes.

2) Measurement of cell viability using MTT assay

The primary cultured B16F10 cells and fibroblast cells were diluted to 4 x 10 ⁴ cells / mL, and then plated in 96-well microplates at 0.1 mL / well to obtain melanoma cells and fibroblast cells. Were incubated at 37 ° C for 24 hours under 5% CO ₂ . Then, 200 μL of 0.5% DMSO was added to the blank, and each of the extracts dissolved in 0.1% DMSO was treated at 1, 2, 5, 10, 25, 50, 100 and 200 μg / mL. After incubation for 24 hours, the culture solution was removed and 50 μL of 2.5% formazan solution was added to each well. After 4 hours of reaction, 100 μL of DMSO was added to dissolve formazan crystals and the optical density at 540 nm was measured with an ELISA reader. OD) was measured to calculate cell viability (%).

Absorbance of sample-blank / Absorbance of control-blank x 100

I. Measuring Convergence Activity

The astringent activity was measured by using hemoglobin similar to skin protein, mixing each sample solution and hemoglobin solution in a ratio of 1: 1, shaking, centrifuging at 1,500 rpm for 3 minutes Absorbance was measured at 576 nm. The astaxanthin activity measurement was expressed by the absorbance reduction rate of the sample solution addition group and the no addition group.

Astringent activity (%) = (1 - Absorbance of sample / Absorbance of control) × 100

4. Statistical Analysis

All samples were prepared twice and repeated 3 times. The mean and standard deviation were calculated using the SPSS statistical program ver. 12.0 and the significance ( p < 0.05) was confirmed by Duncan's multiple test .

Example  1: situation mycelium Cultivated Fatty acid composition

The results of analysis of the fatty acids in the cultured mycelium of the condition were as shown in Table 1. As a result of fatty acid analysis of the samples, seven kinds of fatty acids such as linoleic acid, linolenic acid, palmitic acid and stearic acid were detected, Linoleic acid was the fatty acid which showed the significant difference in cultivation. The amount of fatty acid in the control and the amount of fatty acid in black rice was 38.72 ± 1.14 area and 36.96 ± 0.29 area%, respectively. The content of fatty acid was increased compared to that of control and black rice. The content of fatty acid before and after incubation did not show any significant difference.

The fatty acid composition of cultured mycelium Fatty acid Red rice RRCP ¹ Black rice BRCP ² Myristic acid ^d 0.00 ^{b d} 0.00 ^{b d} 0.14 0.00 ^{a d} 0.00 ^b Palmitic acid ^c 16.23 + - 0.42 ^{b c} 13.19 + - 0.21 ^{c c} 19.30 0.93 ^{a c} 16.27 + -0.53 ^b Palmitoleic acid ^d 0.00 ^{b d} 0.29 + 0.01 ^{a d} 0.00 ^{b d} 0.00 ^b Stearic acid ^d 0.82 + 0.03 a ^{b d} 0.61 0.02 ^{c d} 1.56 0.05 ^{b d} 0.53 0.02 ^a Oleic acid ^a 43.75 ± 1.57 ^{b b} 38.10 + - 1.66 ^{c a} 41.41 + 1.09 ^{a a} 44.54 + - 0.51 ^b Linoleic acid ^b 38.72 + - 1.14 ^{b a} 47.64 + 1.07 ^{a b} 36.96 + -0.29 ^{c b} 38.56 ± 0.17 ^b Linolenic acid ^d 0.59 0.05 ^{a d} 0.00 ^{b d} 0.57 + 0.01 ^{a d} 0.29 0.30 ^b

¹⁾ Red rice cultivated with Phellinus baumii mycelium)

²⁾ Black rice cultivated with Phellinus baumii mycelium)

There is a significant difference ( p <0.05) in the same column (first superscript) or other lower case in the same row (last superscript).

Example  2: situation mycelium Cultivated  Polyphenol content

As shown in Table 2, the polyphenol contents of the test materials were 1244.32 ± 11.80 mg / g of the cultured hyphae, 570 ± 1.56 mg / g of red rice, 363.88 ± 10.66 mg / g of red rice, 162.33 ± 2.71 mg / g of black rice Respectively. This suggests that the polyphenol content is further increased by culturing the mycelium of each state in red and black rice.

The polyphenol content (mg / g) Total polyphenol Red rice RRCP ¹ Black rice BRCP ² Content 363.88 + - 10.66 ^c 1244.32 +/- 11.80 ^a 162.33 ± 2.71 ^d 570 ± 1.56 ^b

¹⁾ Red rice cultivated with Phellinus baumii mycelium)

²⁾ Black rice cultivated with Phellinus baumii mycelium)

Other lower case letters in the same row (superscript) have significant differences ( p <0.05).

Example  3: In vitro Safety check at

Cell viability is shown in Figures 2 and 3. In the case of B16F10 cells, the redness was 1.58 ± 0.09%, 1.50 ± 0.11%, 1.44 ± 0.16%, 1.40 ± 0.13% and 1.30 ± 0.17% at the concentrations of 1, 5, 10, 50 and 100 ㎍ / 1.14 ± 0.12%, 1.40 ± 0.13%, 1.75 ± 0.13%, 1.75 ± 0.13%, 1.75 ± 0.09% and 1.53 ± 0.20% 1.35 ± 0.07% and 1.51 ± 0.07%, 1.44 ± 0.14%, 1.40 ± 0.12%, 1.40 ± 0.10% and 1.35 ± 0.07%, respectively. Therefore, the survival rate was close to 90% at the concentrations of 1, 5, 10, 50, and 100 ㎍ / mL in the rice cultivars of red rice, black rice,

At the concentration of 1, 5, 10, 50, and 100 ㎍ / mL for fibroblast cells, the redness was 2.11 ± 0.17%, 2.17 ± 0.16%, 2.08 ± 0.18%, 2.03 ± 0.15%, and 1.86 ± 0.28% , 2.02 ± 0.16%, 2.02 ± 0.16%, 2.00 ± 0.16%, 1.98 ± 0.16%, and 1.86 ± 0.24% for black rice, 2.03 ± 0.11%, 2.00 ± 0.13%, 1.92 ± 0.11% and 1.93 ± 0.24% , And the concentration of the strain was 90% at a concentration of 100 μg / mL or less, and the concentration of the strain was 2.03 ± 0.11%, 2.00 ± 0.13%, 1.92 ± 0.12%, 1.93 ± 0.10%, and 1.87 ± 0.12% %, Indicating low cytotoxicity (Fig. 3).

Example  4: convergence Astringent ) Active confirmation

Convergence means basically wrinkled or refractory, and astringent agent shows strong protein binding properties. Convergent action has the effect of forming an insoluble film on the surface of skin and mucous membranes and consequently protecting the locus or densifying the tissue to reduce the permeability of the cell membrane. In this experiment, the convergence effect was determined according to the degree of hemoglobin protein binding to the extract.

In the case of cultivated hyphae, 72.14 ± 0.22% was observed at 5000 ㎍ / mL, 55.06 ± 0.64% at redness, 93.53 ± 2.90% in case of cultured hyphae, and 87.96 ± 0.95% The activity was higher in mycelial cultures and 95.52 ± 0.72% in the case of tannic acid, a positive control, showing similar activity to that of cultured mycelium (FIG. 4).

A cosmetic composition containing the cultured Mythaceae mycelium extract obtained by confirming that the safety and convergence effect is excellent through the above examples is prepared and presented. However, cosmetic compositions containing the composition of the present invention are not limited to the following formulation examples. The following formulation examples can be used for the black rice extract of cultured mycelium of mycelia of mushroom or the cultivated mash extract of mushroom mycelium.

Formulation Example  1: softening longevity

The following is an example of the formulation of softening longevity in the cosmetic containing the extract of Mycelia mushroom mycelium extract.

Longevity compounding ingredients ingredient Content (unit:% by weight) Mycorrhizae mycelium culture extract 2.0 glycerin 5.0 1,3-butylene glycol 3.0 PIZZY 1500 1.0 Allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaruronate 5.0 ethanol 10.0 Octidodeces-16 0.2 Polysorbate 20 0.2 Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100

Formulation Example  2: convergent lotion

Examples of formulations of convergent lotion among the cosmetic compositions containing the mushroom mycelium cultured extract are as follows.

Converting lotion formulation ingredients ingredient Content (unit:% by weight) Mycorrhizae mycelium culture extract 2.0 glycerin 2.0 1,3-butylene glycol 2.0 Allantoin 0.2 DL-Panthenol 0.2 Lee D. TEE-2NA 0.02 Benzophenone-9 0.04 Sodium hyaruronate 3.0 ethanol 15.0 Polysorbate 20 0.3 Location of Hazel extract 2.0 Citric acid a very small amount Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100

Formulation Example  3: Nourishing lotion

Examples of formulations of nutritional lotion among the cosmetic compositions containing extracts of Mycelia cultured Mycelium of mushroom are as follows.

Nourishing lotion formulation ingredients ingredient Content (unit:% by weight) Mycorrhizae mycelium culture extract 2.0 Glyceryl stearate SE 1.5 Stearyl alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoin 2.0 Dimethicone 0.8 Tocopheryl acetate 0.5 Carboxyvinyl polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaruronate 5.0 Triethanolamine 0.12 Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100

Formulation Example  4: Nourishing cream

Example of formulation of nutritional cream among cosmetics containing extract of mushroom mycelium culture extract is as follows.

Nourishing Cream Ingredients ingredient Content (unit:% by weight) Mycorrhizae mycelium culture extract 2.0 Pro-type glycerin monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaruronate 4.0 Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100

Formulation Example  5: Massage cream

Example of formulation of a massage cream in a cosmetic composition containing a mushroom mycelium culturing extract is as follows.

Massage Cream Formulation ingredient Content (unit:% by weight) Mycorrhizae mycelium culture extract 2.0 Chin type glycerin monosuccinic acid 1.5 Stearyl alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl isoserate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanolamine 0.7 Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100

Formulation Example  6: Essence

Examples of the formulations of the essences in the cosmetics containing the extract of the Myzanji mycelium culture are as follows.

Essence compounding ingredient ingredient Content (unit:% by weight) Mycorrhizae mycelium culture extract 2.0 glycerin 10.0 Betaine 5.0 PIZZY 1500 2.0 Allantoin 0.1 DL-Panthenol 0.3 Lee D. T.-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaruronate 8.0 Carboxyvinyl polymer 0.2 Triethanolamine 0.18 Octyldodecanol 0.3 Octyldodec-16 0.4 ethanol 6.0 Preservative, fragrance, pigment a very small amount Distilled water Balance Sum 100

Formulation Example  7: Pack

Examples of the formulations of packs in cosmetics containing extracts of Mycelium of mushroom mycelium are as follows.

Essence compounding ingredient ingredient Content (unit:% by weight) Mycorrhizae mycelium culture extract 2.0 Polyvinyl alcohol 15.0 Cellulose sword 0.15 glycerin 3.0 PIZZY 1500 2.0 Cyclostristin 0.15 DL-Panthenol 0.4 Allantoin 0.1 Monoammonium glycyrrhizin acid 0.3 Nicotinamide 0.5 ethanol 6.0 FYGEN 40 Hardened castor oil 0.3 Fragrance, coloring, preservative a very small amount Distilled water Balance Sum 100

Claims (5)

Phellinus baumii A cosmetic composition having pore-shrinking action by densifying skin tissue containing an extract of Mycelium as an active ingredient. 2. The method according to claim 1, wherein the Phellinus baumii mycelium extract Phellinus baumii mycelium was inoculated to the sterilized cereal grains and cultured at 28 to 32 ° C. The cultured mycelia of the mycelia were lyophilized, pulverized, extracted with ethanol, and concentrated under reduced pressure at 45 to 55 ° C Wherein the skin tissue is densified to have a pore-shrinking action. [Claim 3] The cosmetic composition according to claim 1, wherein the Phellinus baumii mycelium extract has an increased pore size of linoleic acid or polyphenol content compared to rice extract. The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of softening agents, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, , A body oil, and a body essence. The cosmetic composition according to claim 1, wherein the composition has a pore-shrinking action. Phellinus baumii A functional food having pore-shrinking action by compacting the skin tissue containing the mycelium cultivation extract as an active ingredient.

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