KR101700872B1 - Cosmetic composition comprising the very high-pressure extract of wormwood fermentation - Google Patents

Abstract

The present invention relates to a method for producing The present invention relates to a cosmetic composition containing an extract of a hyperpigmented extract of a foxtail obtained by fermentation, ultra-high pressure treatment and extraction of an annua , and more particularly, to a cosmetic composition containing an extract of a foxtail selected from the group consisting of yeast, lactic acid bacteria, Bacillus sp. The fermentation product is fermented using a seed or higher and the fermented product is subjected to ultra high pressure extraction method at a temperature of 40 to 90 DEG C at a pressure of 100 to 3000 MPa for 1 to 24 hours to obtain a flavonoid in an active ingredient at a high yield. And an extract thereof. INDUSTRIAL APPLICABILITY According to the present invention, it has excellent antioxidative effect and wrinkle-reducing effect, and has excellent stability without discoloration, separation and precipitation with respect to temperature change.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition containing a high-pressure extract,

The present invention relates to a cosmetic composition excellent in antioxidative effect and wrinkle-reducing effect.

Skin aging is caused by oxidative damage of biomaterials by free radicals. That is, reactive radicals such as active oxygen such as hydrogen peroxide, hydroxy radical, and superoxide radical peroxidize the lipid constituting the cell membrane to destroy the cell membrane, oxidize the protein, attack the nucleic acid and cause oxidative damage, Destroys and eventually kills cells.

Thus, prevention or delay of aging of the skin becomes possible by suppressing the oxidative damage of the biomaterial.

The goal of cosmetics is to maintain beautiful and healthy skin, and also to delay the aging of naturally occurring skin as it gets older. Skin characteristics that are most noticeable due to aging include wrinkles, decreased elasticity, and skin dullness. In addition, there is a phenomenon in which fine wrinkles occur on the skin and the elasticity decreases due to aging. For this purpose, researches are being actively carried out to develop materials having excellent safety and skin beauty effect by searching skin physiological activity of various natural vegetable persons.

To date, substances known to have antioxidant activity include ascorbic acid, dl-alpha-tocopherol, beta-carotene, and dibutylhydroxyltoluene (BHT).

However, since these substances are used as synthetic products, they may cause problems of skin safety when they are used for a long period of time or in a large amount. Therefore, there is an urgent need to develop new antioxidative cosmetics that can be applied safely and effectively to skin. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials.

Artemisia annua is a perennial herb belonging to the genus Mugwort, which is a member of the mugwort tree, and its other names are 黄花 蒿, 호 蒿 香, 香 草,尿 蒿), Hwangryeongho (黄 香 蒿), the foliage (鷄 葉), 胡 호 (秋 蒿), mugwort leaves, such as dogs are called. There are no hairs on the whole grass, it has a peculiar odor, and the stem is green with many branches. Leaves are alternate, 2-3 times narrow, diverged deeply into flags. Its length is 4-7cm and its shape is lanceolate. It has fine hairs and glands on its surface. The center of the leaf is comb-shaped and the upper leaf is small. The flowers bloom in June-September with a greenish-yellow color, and the small heads are gathered to form a cone flower. It is a ball shaped and 1.5 cm in diameter. The involucre sculpture has no hairs and is arranged in 2 to 3 lines, and the outer sculpture is long oval and green. Fruits are ca. 0.7 mm in length and ripen in September to light brown.

It is known to have the effects of bright sunshine, bad temper, paralysis, and sedation in one room. It is distributed in Korea, Japan, Taiwan, Mongolia, Siberia and others.

The main active ingredients of this mugwort are flavonoids, artemisinin derivatives, terpenoides, and a large amount of essential oils.

To date, various pharmacological effects of arthymisinin derivatives and flavonoids have been studied. In particular Artemisia can carbonate (Artesunate) is a semi-synthetic derivative of artemisinin robin mugwort active ingredient has a significant therapeutic effect against multi-drug resistant malaria (Artesunate for the Treatment of Severe Falciparum Malaria N Engl J Med 2008;... 358 : 1829-1836)

In addition, the antitumor activity of artesanate was investigated. Leukemia and colorectal cancer were the most effective antiresorptive agents. In addition, it shows antitumor activity against cancer cells resistant to general anticancer drugs and has been studied as a promising candidate for cancer treatment due to its low toxicity. The plonoid components of mugwort are also known to exhibit anti-malarial effects and are used in therapeutic agents. (The anti-malarial artesunate is also active against cancer Int J Oncol 2001; 18 (4):.. 767-73)

Accordingly, the present inventors intend to provide a cosmetic composition containing a flavonoid component at a higher yield than the conventional art, and having excellent antioxidative and wrinkle-reducing effects.

In order to accomplish the above object, the present invention provides a cosmetic composition containing an extract obtained from fermentation of mugwort, followed by ultra-high pressure treatment and extraction, and a milk powder mugwort fermented ultra-high pressure extract.

Preferably, the fermentation is a bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus subtilis, and a mixture thereof, which is fermented for 1 to 7 days while maintaining a pH of 5 to 7 under a temperature condition of 20 to 40 캜 Wherein the cosmetic composition is a cosmetic composition.

Further, preferably, the cosmetic composition is characterized in that the yeast is Saccharomyces cerevisisae.

Preferably, the cosmetic composition is obtained by treating the ultra-high pressure treatment at a pressure of 100 to 3000 MPa at a temperature of 40 to 90 DEG C for 1 to 24 hours.

Preferably, the extraction is carried out using at least one solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol and hexane And extracting the cosmetic composition.

In addition, it is preferable that the milk powder-containing ultra-high pressure extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

In addition, the cosmetic composition is preferably characterized in that the cosmetic composition gives an antioxidative effect.

Further, it is preferable to provide a cosmetic composition characterized in that the cosmetic composition is used for improving wrinkles.

Still another object of the present invention is achieved by a cosmetic method characterized in that the cosmetic composition is applied to human skin to give an antioxidative effect or to help improve wrinkles.

INDUSTRIAL APPLICABILITY The present invention can provide a cosmetic composition having an increased flavonoid content and excellent antioxidative effect and wrinkle-reducing effect.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The term " drug " is used in reference to a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight, or length of 30, 25, 20, 25, 10, 9, 8, 7, 6, Level, value, number, frequency, percentage, dimension, size, quantity, weight or length of a sample,

Throughout this specification, the words " comprising " and " comprising ", unless the context clearly requires otherwise, include the steps or components, or groups of steps or elements, And that they are not excluded.

The inventors of the present invention have investigated the possibility of application as a cosmetic composition containing a fermentation extract of P. falciparum from various natural products, and found that antioxidative and wrinkle-improving effects can be measured through various experiments, and as a result, the efficacy as a cosmetic product can be expected. The ingredients of the flavonoid having such excellent effects were obtained at the highest possible yields, and a cosmetic composition having more excellent effects was developed.

The present invention relates to a cosmetic composition containing a hypericum extract of fermented milkweed obtained by fermentation, ultra-high pressure treatment and extraction of milkworm, and more particularly to a cosmetic composition comprising a milkworm selected from the group consisting of yeast, lactic acid bacteria, Bacillus subtilis, A cosmetic composition containing fermented microorganism fermented and treated with ultrahigh pressure to increase the content of flavonoid component and having an extract of Fusarium oxysporum fermentation extract as an active ingredient.

The cosmetic composition of the present invention as described above is a cosmetic composition containing an extract of hypericum perilla mugwort obtained by fermentation of dog mugwort, followed by ultra-high pressure treatment and extraction.

The cosmetic composition of the present invention comprising the above components is suitable as a cosmetic composition for antioxidant effect and wrinkle improvement of skin.

In order to obtain a flavonoid component having various effects at the highest possible yield, the present invention has been subjected to ultrahigh pressure treatment and extraction process after fermentation of mugwort. By this treatment, it was confirmed that the content of active ingredient was increased, and it was confirmed that antioxidative effect and wrinkle-reducing effect were obtained by applying it to cosmetics, thereby completing the present invention.

In the cosmetic composition of the present invention, the extract of the hyperpigmented extract of mugwort is fermented with at least one bacterium selected from the group consisting of yeast, lactic acid bacterium and Bacillus subtilis at a temperature of 20 to 40 ° C and maintained at a pH of 5 to 7 And fermenting it for 1 to 7 days, more preferably by using Saccharomyces cerevisisae, and most preferably by using Saccharomyces cerevisiae KCTC 7904, I will.

The mugwort used in the present invention was prepared by a generally known method. The mugwort was thoroughly washed, ground, and then finely ground using 300 mesh. The ground shit mugwort was added to the culture medium of Saccharomyces cerevisiae (Saccharomyces cerevisiae KCTC 7904) at 100 g / L. 1 to 4%, preferably 1 to 2%, of glucose was added as a carbon source, and 0.1 to 1%, preferably 0.2 to 0.5%, of peptone was further added and cultured. The cultivation was carried out using a 5 L fermenter at a temperature of 20 to 40 ° C and for 1 to 7 days while maintaining the pH at 5-7. After culturing, the culture was centrifuged to remove the cultures first.

Preferably, the ultra-high pressure treatment is carried out at a temperature of 40 to 90 DEG C at a pressure of 100 to 3000 MPa for 1 to 24 hours, preferably at a pressure of 500 to 1000 MPa at a temperature of 40 to 60 DEG C, To 24 hours, most preferably at a pressure of 1000 MPa and at a temperature of 50 < 0 > C for 24 hours.

Preferably, the extraction is carried out in the presence of at least one extraction solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol and hexane , More preferably extracted with ethanol, 70% ethanol aqueous solution or water, most preferably with 70% ethanol aqueous solution.

Ethanol was added to the final extract to obtain a final 70% (v / v) ethanol aqueous solution. The extract was refluxed three times for 5 hours, cooled, and filtered through Whatman # 2 filter paper. When the filtration is completed, the extract obtained in the extracting step is transferred to the concentration tank, concentrated under reduced pressure, and lyophilized. Concentration under reduced pressure was performed using a rotary vacuum concentrator. Concentration conditions were as follows: 700 ml of extract was put into a 1,000 ml round-bottomed flask, and the mixture was concentrated under reduced pressure at 650 to 750 mmHg at 50 to 70 ° C and 600 to 1200 rpm for 2 hours Respectively. The concentrate under reduced pressure was preliminarily frozen to reduce the temperature to -40 ° C, keep it at -40 ° C for 2 to 4 hours, drop to -80 ° C through trap freezing, maintain the vacuum at 10 to 30 mTorr Raise to -40 ° C over 120 minutes. Then, the temperature was maintained at -20 ° C for 240 minutes, at 0 ° C for 40 minutes, at 15 ° C for 340 minutes, at 30 ° C for 130 minutes, and then maintained at 1300 minutes until completely dried.

It is preferable that the extract is a extract obtained by ultra-high pressure treatment of the fermentation product obtained by fermentation of milk powder, or a powder obtained by vacuum-concentrating the extract, lyophilized, or a combination of the powder with an extraction solvent.

In addition, although the extract of the hyperpigmented extract of P. japonica is not limited to a specific range, it is preferably contained in an amount of 0.0001 to 30% by weight based on the total weight of the cosmetic composition. When the content of the extract is less than 0.0001% by weight, the effect of improving wrinkles is not exhibited. When the content of the extract is more than 30% by weight, the effect of increasing the content of the extract is insignificant, and there is a problem in safety and stability.

The ingredients contained in the cosmetic composition of the present invention include, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, such as conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, .

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention, and it is to be understood by those skilled in the art that the present invention is not limited thereto It will be obvious.

< Example  1> Production of ultra-high-pressure extract of mugwort mugwort

The mugwort was selected as a sample, thoroughly washed, and finely pulverized so as to pass through a 300 mesh sieve.

The powdered ground wormwood, which had been pulverized so as to have a weight of 10 g / L, was added to the cultivation medium of Saccharomyces sp. (Saccharomyces cerevisiae KCTC 7904). 2% (W / V) of glucose was added as a carbon source and 0.5% (w / v) of peptone was further added thereto. The cultures were incubated for 5 days at 37 ° C and pH 6 using a 5 L fermenter. After culturing the microorganism, the culture was centrifuged and the culture was firstly removed.

The fermented milk powder of which the culture was firstly removed was heated to 50 ° C under a pressure of 1000 MPa using an ultrahigh pressure processor, treated at ultra high pressure for 24 hours, and then cooled to room temperature.

Ethanol was added to the ultrapure water-treated fermented milk to make a final 70% (v / v) ethanol solution. The mixture was refluxed three times for 5 hours, cooled, and filtered through Whatman # 2 filter paper.

When the filtration is completed, the extract obtained in the extracting step is transferred to a concentration tank and concentrated under reduced pressure at 50 DEG C or below, followed by lyophilization. The lyophilized product was dissolved in 50% glycerin to a concentration of 2% to prepare an extract (Example 1).

< Comparative Example  1> Preparation of Mugwort extract

Comparative Example 1 is a general organic solvent extract of mugwort, and mugwort was selected as a sample, washed thoroughly, and then pulverized and passed through a 300-mesh sieve to finely pulverize the pulverized material. Ethanol was added to make a final concentration of 70% (v / v) ethanol aqueous solution, refluxed three times for 5 hours, cooled down, and filtered through a filter of whatman # 2. The filtered extract was concentrated under reduced pressure and lyophilized. The powder was dissolved in 50% glycerin to a concentration of 2% (W / V) to prepare a dog powder (Comparative Example 1).

< Comparative Example  2> Preparation of fermented milk extract

Comparative Example 2 was prepared by fermenting the mugwort, and the mugwort was selected as a sample, washed thoroughly, and finely pulverized to pass through a 300-mesh sieve. This was added to the cultivation medium of Saccharomyces sp. (Saccharomyces cerevisiae KCTC 7904) to a concentration of 10 g / L. 2% (W / V) of glucose was added as a carbon source and 0.5% (w / v) of peptone was further added thereto. The cultures were incubated for 5 days at 37 ° C and pH 6 using a 5 L fermenter. After culturing, the culture was centrifuged to remove the cultures first.

Ethanol was added so that the culture broth was firstly removed to make a final 70% (v / v) ethanol aqueous solution, refluxed three times for 5 hours, cooled, and then filtered with a filter of Whatman # 2 Respectively. After the filtration was completed, the extract was transferred to a concentration tank, and the filtered extract was concentrated under reduced pressure and lyophilized. The powder was dissolved in 50% glycerin to a concentration of 2% (W / V) to prepare a powdery mildew fermented extract (Comparative Example 2).

< Comparative Example  3> Production of Extra High Pressure Extract of Dogwood Mugwort

The mugwort was selected as a sample, washed thoroughly, and finely ground to obtain a 300 mesh sieve. The mugwort powder was heated to 50 ° C under a pressure of 1000 MPa using an ultra-high pressure processor, treated for 24 hours, Lt; / RTI &gt;

Ethanol was added to the final extract to obtain a final 70% (v / v) ethanol solution. The extract was refluxed three times for 5 hours, cooled, and filtered through Whatman # 2 filter paper. When the filtration is completed, the extract obtained in the extracting step is transferred to the concentration tank, and the filtered extract is concentrated under reduced pressure and lyophilized. The lyophilized product was dissolved in 50% glycerin to a concentration of 2% (W / V) to prepare a hyperpigmented extract of Clostridium perfringens (Comparative Example 3).

< Experimental Example  1> Determination of active ingredient content of mugwort

The content of each of the flavonoids of the artichoke extract of Example 1 and Comparative Examples 1 to 3 was analyzed and the results are shown in Table 1 below.

Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 content Flavonoid 2.98 2.02 2.04 2.45 %

As a result of measurement of the active ingredient content of the Lauroginae Extract of Example 1 and Comparative Examples 1 to 3, it was confirmed that the flavonoid component content of Example 1 was significantly higher than that of Comparative Examples 1 to 3.

From the above results, it was found that the flavopithex powder extract extracted from the processing conditions (Example 1) of the present invention extracting by high pressure treatment after fermentation greatly increased the flavonoid components compared with other extraction conditions (Comparative Examples 1 to 3) I could confirm.

< Experimental Example  2> Antioxidant effect measurement

The antioxidative activities of the extracts prepared in Example 1 and Comparative Examples 1 to 3 of the present invention were measured by the Tiobarbituric acid method (TBA assay) using a lipid peroxidation system by Fenton reaction . That is, Example 1 and Comparative Example 1 were carried out in 5 ml of 25 mM Trizma hydrochloric acid / 0.75 mM potassium chloride buffer containing 10 占 퐇 of ethyl linoleate, 0.2% sodium dodecylsulfoxide (SDS), 10 占 퐉 ol of ferrous chloride and 2 占 퐉 ol of hydrogen peroxide The dimethylsulfoxide (DMSO) test solution of Lauroginae japonica extract prepared in [3] or [3] was added, and the mixture was incubated at 55 ° C for 16 hours. Then, 50 μl of 4% dibutylhydroxyltoluene (BHT) was added to stop the reaction. Add 0.3 ml of this solution to a test tube, add 3.0 ml of 0.05 N hydrochloric acid, 0.67% thiobarbituric acid (TBA) solution, and boil for 30 minutes. After cooling the reaction solution, 4.0 ml of 85% butanol was added, and the absorbance of the butanol layer after centrifugation was measured at 535 nm. The antioxidative effect of each extract was calculated by the following formula.

The results are shown in Table 2 below.

Test substance Antioxidative effect(%) Experimental concentration (10 / / ml) Experimental concentration (50 / / ml) Experimental concentration (100 / / ml) Example 1 36 63 85 Comparative Example 1 26 45 64 Comparative Example 2 24 46 67 Comparative Example 3 30 54 70

 The antioxidative activities of the extracts of Example 1 and Comparative Examples 1 to 3 showed high activity. The extracts of Example 1 and Comparative Examples 1 to 3 and the conventional antioxidants such as ascorbic acid, The antioxidative activities of dl-alpha-tocopherol, beta-carotene and dibutylhydroxyltoluene (BHT) were measured by the same method as Experimental Example 2 to compare the IC50 of 50% inhibitory concentration.

The results are shown in Table 3 below.

Test substance IC ₅₀ (10 μg / ml) Example 1 38.2 Comparative Example 1 48.5 Comparative Example 2 46.8 Comparative Example 3 43.2 Ascorbic acid 21.90 Dl-alpha-tocopherol &lt; / RTI &gt; 3.36 Beta-carotene 29.8 Dibutylhydroxyltoluene (BHT) <1

The rape extracts of Example 1 and Comparative Examples 1 to 3 showed high activity comparable to other antioxidants. Especially, it can be seen that these extracts have significant antioxidant activity despite being a mixture of natural products.

< Experimental Example  3> Evaluation of Wrinkle Reduction Effect

The wrinkle-improving effect of the cream of Comparative Example 1 to Comparative Formulation Example 1 to Formulation Example 1 of the hyperpigmented extract of fermented milk powder of mugwort mugwort was evaluated as follows.

Forty subjects (35 to 50 year-old women) of the experiment used cream prepared in Formulation Example 1 on the right side of the face, cream twice in Comparative Formulation Examples 1 to 3 on the left side of the face, The wrinkle improvement effect after 6 weeks was visually evaluated to confirm the wrinkle improvement degree. The results of wrinkle improvement based on this evaluation are shown in Table 4 below

Experimental product Wrinkle improvement effect Effective rate (%) Great slightly none Formulation Example 1 7 2 One 83.3 Comparative Formulation Example 1 4 3 3 58.4 Comparative Formulation Example 2 5 3 3 61.5 Comparative Formulation Example 3 5 3 2 66.7

 As shown in Table 4, it was found that the skin wrinkle-reducing effect of the cream-applied cream containing the hyper-high pressure extract of mugwort mugwort was excellent. Skin irritation could not be observed in most of the subjects who applied this cosmetic composition to the skin.

Table 5 shows the prescription examples of the cream in cosmetics containing extract of Artemisia princeps. Herein, the dog's mugwort extract refers to Example 1 and Comparative Examples 1 to 3.

ingredient Content (unit:% by weight) Mugwort extract 5.0 glycerin 5.0 Sodium hyaruronate 4.0 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (7)

A cosmetic composition having an antioxidative or wrinkle-reducing effect, which contains fermented milk powder, fermented with Saccharomyces cerevisiae, and then subjected to ultra-high pressure treatment and extraction at a pressure of 1000 MPa, The method according to claim 1,
Wherein said fermentation is carried out by fermenting said gallmopsis with saccharomyces cerevisiae for 1 to 7 days while maintaining the pH at 5 to 7 under a temperature condition of 20 to 40 占 폚. The method according to claim 1,
Wherein said ultra high pressure treatment is carried out at a pressure of 1000 MPa and a temperature of 40 to 90 DEG C for 1 to 24 hours. The method according to claim 1,
The extraction is carried out by using at least one solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol and hexane . The method according to claim 1,
The cosmetic composition according to any one of claims 1 to 3, wherein the milk powder is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. delete delete