KR20170074821A - Anti-Oxidant Composition Using a Fermented Materials or an Extract of Korean Dendropanax - Google Patents

Abstract

The present invention discloses a composition for antioxidation using an extract of Huaculi or a fermented product, which has been confirmed to have an excellent antioxidative activity by the DPPH method (Nature 181: 1198-1200, 1958).

Description

[0001] The present invention relates to a composition for antioxidation,

The invention hwangchil tree (Dendropanax morbifera ) extract or a fermented product thereof.

The evergreen broad-leaved tree belonging to Araliaceae is native only in southern part of South Korea such as Haenam Wando in the world and in Jeju-do, and it does not fall in winter. .

Hwangchil has been used since ancient times as a rare paint that emits gold of the emperor's armor, helmet and other metal ornaments.

In addition to these paint functions, Huangchil has a neuro-stabilizing effect as a benzodiazide (Lee Young-min, Jangheung County Agricultural Technology Center, 2012), and it is said that Ishin's basal gangmok is effective for burning removal, eye quality and burn treatment, paralysis, leprosy, Its roots and stems have been known to have efficacy in the treatment of migraine, hypertension, dysentery, migraine, and menstrual irregularities (J. Korean Soc. Agric. Chem. Biotechnol. 43: 63-66, 2000). In addition, it is known that β-selinene belonging to sesquiperpenes is the most abundant in the Hwaseongchil, and capnellane-8-one is the most abundant in the latter (J. J. Biotechnol. Bioeng. 10: 393-400, 1995) .

Recently, physiological activities such as skin whitening activity, anticancer activity, anti-inflammatory activity, hangover resolution function, and vineyard activity of Hwacheon-gil extract have been reported (Park, Soo Ae et al., Korean Society for Microbiology Biotechnology 41 (4): 407-415, 2013 , Korea Unexamined Patent Publication No. 2014-0070470, Korean Registered Patent No. 1283775), health supplements, food additives and the like (Korea Patent No. 1162699; Korean Patent No. 1333007).

The present invention discloses the antioxidant activity of the extracts or the fermented products of D. torus.

It is an object of the present invention to provide an antioxidative composition using an extract of Huangchu tree or a fermented product.

Other and further objects of the present invention will be described below.

As shown in the following Examples and Experimental Examples, the present invention can be used to produce extracts from 70% ethanol or hot-water extracts of Leucumber leaf and stem, and to ferment lactic acid bacteria, Bacillus subtilis, After extracting water by obtaining water and extracting it with hot water, the antioxidant activity of the extract and fermented extract was confirmed by the DPPH method (Nature 181: 1198-1200, 1958).

In view of the above, the present invention can be understood as an antioxidative composition comprising an extract of Hwangcholgulchus or a fermented product as an active ingredient.

In the present specification, the term "Horticultural extracts" refers to extracts of Huangchu tree, leaves, fruits, flowers, roots, or a mixture thereof in water, lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, and butanol, (DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof is used as the organic solvent, for example, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate Extracts obtained by extracting with supercritical extraction solvents such as carbon dioxide and pentane or fractions obtained by fractionating the extracts, and the extraction method refers to a method of extracting the extracts obtained by extracting the extracts using a method of extracting the extracts using a method of extracting , Heating, ultrasonic irradiation, supercritical extraction, and the like can be applied. In the case of the fractionated extract, the fraction is suspended in a specific solvent and then mixed with a solvent having a different polarity. The extract is adsorbed on a column packed with silica gel or the like, and then a hydrophobic solvent, a hydrophilic solvent, Quot; means fractions obtained as a mobile phase. Also, the meaning of the extract includes a concentrated liquid extract or a solid extract in which the extraction solvent is removed by a method such as freeze drying, vacuum drying, hot air drying, spray drying and the like. Preferably an extract obtained by using water, ethanol or a mixed solvent thereof as an extraction solvent, more preferably an extraction solvent obtained by using a mixed solvent of water and ethanol or hot water as an extraction solvent.

In the present specification, the term "fermented product" means a fermented product obtained by inoculating a lactic acid bacterium (particularly Lactobacillus brevis ) or Bacillus subtilus with or without adding a carbon source to the yellowtail tree to be fermented, And then adding water). This culture may be used as it is or may be used in liquid or powder form by concentration under reduced pressure and / or lyophilization, or may be used in water, a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol and butanol, methylene chloride, ethylene, acetone, , And extracted with chloroform, ethyl acetate, butyl acetate, N, N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or a mixed solvent thereof. When extracted and used, the description on the above extract is applied mutatis mutandis here.

The fermentation product may be cultured after inoculation with the fermenting microorganism at a temperature condition of 15 ° C to 45 ° C, particularly at a temperature of 25 ° C to 40 ° C, and the humidity may be 60 ° C to 20% It can be for 30 days. In the inoculation / cultivation of yeast, it is preferable that the temperature is adjusted to 15 to 45 DEG C, particularly 20 to 40 DEG C, and to 70 to 10%, particularly 70 to 5%, in the same manner as described above. The fermentation period is preferably continued until sufficient fermentation is achieved. In the following examples, the present inventors fermented for 1 month at a temperature of 30 캜 and a humidity of 70 賊 5%.

The antioxidant composition of the present invention may contain the effective ingredient in any amount (effective amount) as long as it exhibits antioxidative activity, depending on the purpose of use, formulation, compounding purpose and the like. Will be determined within the range of 0.001 wt% to 99.990 wt%. Here, "effective amount" refers to the amount of active ingredient capable of inducing an antioxidative effect. Such effective amounts can be determined experimentally within the ordinary skill of those skilled in the art.

The antioxidant composition of the present invention may further contain, in addition to the active ingredient, any compound or natural extract known to have antioxidant activity and safety already proven for the purpose of raising or reinforcing the antioxidant effect. Specifically, such compounds or extracts may be compounds or extracts in accordance with the Act on Health Functional Foods (standards and specifications for health functional foods), compounds that have been individually recognized according to the Act on Health Functional Foods Or extracts such as green tea extract, bamboo leaf extract, melon extract, bramble extract, beeswax alcohol, ubiquinol, coenzyme Q10, tomato extract, grape seed extract, French seaweed extract, red ginseng extract (red ginseng concentrate) have. Such compounds or natural extracts may be included in the compositions of the present invention in combination with one or more of their active ingredients.

In a specific embodiment, the composition of the present invention can be identified as a food composition.

The food composition of the present invention can be prepared in any form and can be used in various forms such as beverages such as tea, juice, carbonated drink, ionic drink, processed milk such as milk and request route, gum, rice cake, Korean confectionery, Such as foods, tablets, capsules, rings, granules, liquids, powders, flakes, pastes, syrups, gels, jellies, bars and the like. In addition, the food composition of the present invention may be classified into any product category as long as it meets the laws and regulations on the time of manufacture and distribution in the legal and functional category. For example, it may be a health functional food according to the Act on Health Functional Foods, or a food functional food according to the Food Sanitation Act (standards for food additives, food standards and specifications), confectionery, pulses, bean oil, fermented beverages, Particularly food preparations for weight control).

The food composition of the present invention may contain food additives in addition to the active ingredients thereof. Food additives are generally understood to be substances which are added to foods and mixed or infiltrated into food in the manufacture, processing or preservation of food, and their safety must be ensured since they are taken with food daily and for a long time. Food additives according to the Food Hygiene Act (food additives notification, food additive standards and standards) are limited by the classification of safe synthetic food additives as chemical synthetic products, natural additives and mixed preparations.

These functional food additives can be classified into sweeteners, flavors, preservatives, emulsifiers, acidifiers, and thickeners.

A sweetener is used to impart a sweet taste suitable for foods, and natural or synthetic sweeteners can be used. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavors may be used to enhance taste or flavor, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. Examples of natural flavoring agents include those obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or those obtained from green tea leaves, Asiatica, Daegu, Cinnamon, Chrysanthemum leaves and Jasmine. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavors may be used depending on the case, and synthetic flavors such as esters, alcohols, aldehydes, terpenes and the like may be used.

As the preservative, calcium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate and EDTA (ethylenediaminetetraacetic acid) can be used. As the emulsifier, acacia gum, carboxymethyl cellulose, Pectin and the like. As the acidulant, math, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid and the like can be used. The acidulant may be added so that the food composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste.

Examples of the thickening agent include suspending agents, sedimentation agents, gel-forming agents, bulking agents and the like.

The food composition of the present invention may contain physiologically active substances or minerals which are known in the art and which are stable as a food additive in addition to the above-mentioned food additives in order to supplement and supplement functional and nutritional properties.

Examples of such physiologically active substances include catechins contained in green tea and the like, vitamins such as vitamin B1, vitamin C, vitamin E and vitamin B12, tocopherol, dibenzoyl thiamine, etc. Examples of minerals include calcium preparations such as calcium citrate, magnesium stearate , Iron preparations such as iron citrate, chromium chloride, potassium iodide, selenium, germanium, vanadium, zinc and the like.

The food composition of the present invention may contain an appropriate amount of the above-mentioned food additives according to the product type so as to achieve the purpose of addition thereof.

With regard to other food additives that can be included in the food composition of the present invention, reference may be made to the Food Code or the Food Additive Code.

In another specific embodiment, the composition of the present invention can be identified as a pharmaceutical composition.

The pharmaceutical composition of the present invention may be prepared into oral formulations or parenteral formulations according to the route of administration by conventional methods known in the art, including pharmaceutically acceptable carriers in addition to the active ingredient. The term "pharmaceutically acceptable" as used herein means that the application (prescribing) subject does not have the above-mentioned toxicity that is adaptable without inhibiting the activity of the active ingredient.

When the pharmaceutical composition of the present invention is prepared into an oral formulation, it may be formulated into powder, granules, tablets, pills, sugar tablets, capsules, solutions, gels, syrups, suspensions, wafers And the like. Examples of suitable pharmaceutically acceptable carriers include sugars such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol, xylitol, starch such as corn starch, potato starch and wheat starch, cellulose, methylcellulose, ethylcellulose, Cellulose derivatives such as sodium carboxymethyl cellulose and hydroxypropylmethyl cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, And the like. In case of formulation, a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, and / or an excipient may be formulated according to need.

When the pharmaceutical composition of the present invention is prepared into a parenteral dosage form, it may be formulated in the form of an injection, transdermal drug delivery, nasal aspirate and suppository together with a suitable carrier according to methods known in the art. Examples of suitable carrier in the case of injection preparation include sterilized water, polyol such as ethanol, glycerol or propylene glycol, or a mixture thereof. Preferably, the carrier is selected from the group consisting of Ringer's solution, phosphate buffered saline containing triethanolamine, , 5% dextrose, and the like may be used. When formulated with a transdermal drug, it can be formulated in the form of an ointment, a cream, a lotion, a gel, a solution for external use, a pasta, a liniment, or an air-roll. The nasal inhalant may be formulated in the form of an aerosol spray using a suitable propellant such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide, etc. The suppositories may be witepsol, polyethylene glycol, cacao butter, laurin, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearates, sorbitan fatty acid esters and the like can be used.

The formulation of pharmaceutical compositions is well known in the art, and more details can be found in Remington ' s Pharmaceutical Sciences (19th ed., 1995) and the like. This document is considered part of this specification.

The preferred dosage of the pharmaceutical composition of the present invention is 0.001 mg / kg to 10 g / kg per day, preferably 0.001 mg / kg to 1 g / day, depending on the patient's condition, body weight, sex, age, / kg < / RTI > The administration can be carried out once or several times a day. Such dosages are not to be construed as limiting the scope of the invention in any respect.

In a specific embodiment, the composition of the present invention can be identified as a cosmetic composition.

Even when the composition of the present invention is identified as a cosmetic composition, the cosmetic composition may be classified into any product category according to the use of the cosmetic composition. Specifically, the cosmetic composition may be a functional cosmetic having a use such as skin whitening, . Specific examples of the product form include a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax Foundation, spray or the like. In a specific product form, it may be a form of flexible lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

The cosmetic composition of the present invention may contain, in addition to its active ingredient, conventional additives such as stabilizers, solubilizing agents, surfactants, vitamins, colorants and antioxidants, and carriers commonly used in cosmetic compositions.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid esters of sorbitan, and the like.

When the formulation of the present invention is a suspension, a carrier, such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention can be produced by a method for producing a cosmetic composition which is usually carried out in the art, except that it contains the active ingredient exhibiting antioxidative activity.

INDUSTRIAL APPLICABILITY As described above, the present invention can provide a composition for antioxidation using the extract of Huangchuang or a fermented product.

The antioxidant composition of the present invention can be made into a food, a cosmetic product or a pharmaceutical product.

Hereinafter, the present invention will be described with reference to Examples, Production Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples, preparation examples and experimental examples.

< Example > Horticultural extract or Fermented  Produce

<Example 1> Preparation of extracts

Table 1 shows the results obtained by lyophilization of the leaves and stems of Tochigi (the same weight of the leaves and stems of Huangchu), followed by pulverization of the dried leaves and stalks, and 2 times the weight of 70% ethanol (ALC) , And the extract was filtered. Next, the filtrate was concentrated under reduced pressure to obtain lyophilized 70% ethanol extract of powdered wood (1-1 to 1-7 in [Table 1] below).

(AQA) was added to the pulverized leaves and stem of the leaves, and the mixture was extracted with a vacuum low-temperature extractor (COSMOS 660, Daejo Industry, Herbang Medical) at 120 ° C for the time shown in [Table 1] below. After filtration, the filtrate was concentrated under reduced pressure and lyophilized to prepare a hot-water extract of Huangchu tree (2-1 to 2-6 in Table 1 below).

(AQA) was added to the extraction residue (filtration after filtration, which is the extraction residue 1-1 of Table 1 below) of the above 70% ethanol extract (manufactured by COSMOS 660, The extract was filtered, and the filtrate was concentrated under reduced pressure and lyophilized to prepare a hot-water extract of the powdery Hwangchu-wood extract residue (hereinafter referred to as &quot; 2- 7).

Extraction solvent and extraction time Sample No. menstruum time 1_1 ALC 4H 1_2 ALC 10H 1_3 ALC 12H 1_4 ALC 17H 1_5 ALC 20H 1_6 ALC 24H 1_7 ALC 48H 2_1 AQA 4H 2_2 AQA 10H 2_3 AQA 12H 2_4 AQA 17H 2_5 AQA 20H 2_6 AQA 24H 2_7 AQA 1-7 after 4H

Example 2 Preparation of Fermented Extracts of Yellow Grass Tree

Lactobacillus brevis prepared from MRS (DeMan, Rogosa, and Sharpe) broth was added to the pulverized material obtained by lyophilizing dried leaves and stems after lyophilization, 2% (v / v) inoculated and fermented for 18 days at a temperature of 60 賊 5% humidity and 36 째 C (i.e., fermented without adding water).

Subsequently, the fermented product was extracted with 2-fold weight of water at 96 ° C for 4 hours using a vacuum low-temperature extractor (COSMOS 660, Daejo Industry, Herbang Medical), and the extract was filtered. The filtrate was concentrated under reduced pressure to obtain lyophilized (Hereinafter referred to as "lactic acid bacterial solid fermentation extract").

In the same manner as described above, fermented extract of Huangchu tree was prepared. Fermentation was carried out by adding 1-fold weight of water to the leaf of Huaculi and the pulverized product of the stem during fermentation and fermenting and extracting it to prepare powdery fermented extract (hereinafter referred to as "fermented lactic acid fermentation extract" .

In addition, fermented extracts of Fusarium oxysporum were prepared in the same manner as in the production of lactic acid bacteria solid phase fermentation extracts, and fermented by inoculation with 2% (v / v) of Bacillus subtilis prepared from nutrient broth medium as a fermenting microorganism, (Fermented extract of Aspergillus oryzae).

In addition, fermented extract of Huangchu tree was prepared in the same manner as in the production of the lactic acid fermentation solid phase fermentation extract, except that Aspergillus prepared in PDB (Potato Dextrose Broth) oryzae ) 2% (v / v) and fermented and extracted to prepare a powdery fermented extract.

< Experimental Example > Antioxidant activity evaluation experiment

The antioxidant activity was measured by the Blois method, which measures the radical scavenging effect of 1,1-diphenyl-2-picrylhydrazyl (DPPH). The DPPH solution was prepared by dissolving about 2 mg of DPPH in 15 mL of ethanol. To 12 mL of this solution was added 6.25 mL of DMSO, and then UV-Vis of the control group at a wavelength of 517 nm. It was diluted with ethanol to an absorbance of 0.94-0.97 and shaken for 10 seconds. To 1 mL of solvent, 1 mg of fermentation extract was mixed with 1 mg of 6 points, and the resulting solution was sufficiently dissolved. Then, 50 μL of the sample solution was added to 450 μL of DPPH prepared and allowed to stand at room temperature for 10 minutes, and the absorbance was measured at 517 nm. The concentration of the sample (IC ₅₀ ) when the absorbance of DPPH was reduced by 50% was indicated, and each sample was repeated three times to obtain an average value. The results are shown in [Table 2] to [Table 4] below.

<Antioxidant Activity of 70% Ethanol Extract>

<Antioxidant Activity of Hot Water Extract>

<Antioxidant Activity of Fermented Extract>

In Table 4, Sample 1 is a solid fermentation extract of lactic acid bacteria, Sample 2 is a liquid fermentation extract of lactic acid bacteria, Sample 3 is a solid fermentation extract of Bacillus subtilis, and Sample 4 is a solid fermentation extract of Aspergillus.

Claims (4)

A composition for antioxidation comprising an extract of Huangchu tree or a fermented product as an active ingredient.
The method according to claim 1,
Wherein the composition is a food composition.
The method according to claim 1,
Wherein the composition is a pharmaceutical composition.
The method according to claim 1,
Wherein the composition is a cosmetic composition.