KR20190036269A - Japanese apricot essential oil and cosmetic composition for skin whitening containing the same - Google Patents

Abstract

The present invention relates to a yellow plum seed essential oil and a whitening cosmetic composition containing the same. The yellow plum seed essential oil has an effect of inhibiting tyrosinase activities, inhibiting melanin production, and reducing pigmentation, thereby being useful as a cosmetic composition for skin whitening. A method of manufacturing the yellow plum seed essential oil of the present invention includes a step of naturally drying and pulverizing yellow plum seed and a step of heating the pulverized seed by water distillation, followed by filtering.

Description

[0001] The present invention relates to a white plum seed essential oil and a whitening cosmetic composition containing the same,

The present invention relates to sulfuric acid seed essential oil and a whitening cosmetic composition containing the same.

Plum tree ( Prunus mume Sieb. Et Zucc) belonging to Rosaceae is originated from Hubei province and Sichuan province in China and has been distributed in Asia including Korea and Japan and has been cultivated for about 3000 years. In China, various parts such as fruit, flowers, branches, leaves and roots of plum trees were used as medicinal materials. Especially, the fruit of plum tree is called plum (梅 实), and it has been used for edible or medicinal purposes since ancient times as plum wine, pickles. The plum is 2 ~ 3cm in diameter and has a lot of hairs on the skin and strong acidity (Lee Young-eun, Hong Seung-heon 2004). Nucleus (nucleus) as the nucleus (nuclear), green or from June to July when it turns yellow to ripe. Plums are divided into 5 types according to harvesting time and processing method (Lee, Young-eun, Hong-Seung Heon, 2004). Cheongmee is a greenish, less ripe plum, harvested in May and June, and has strong sourness and firm flesh.黄梅 (黄梅) is yellow, harvested in June to July, the flesh is soft and fragrant. The gold plum is steamed and dried, and the omu is the plum which is fumigated and blackened. White pears are pickled in salt water and then dried. Chrysanthemum has amygdalin, a cleansing glycoside, which is dangerous when it is inhabited because it produces liquidation (hydrogen cyanide, HCN) by intestinal digestive enzymes when ingested.

Plum is used as a medicinal ingredient in oriental medicine and is used for detoxification, fever, relief of thirst, relieving cough and not digesting (Lee, Eun, Hong, 2004). The plum has a cosmetic efficacy that promotes the secretion of parotin in the parotid glands to make the face and skin moisturize (Yeong-seung, Kim, 2004). The scientific efficacy of plum was evaluated by the combination of influenza A virus suppression (Yingsakmongkon et al 2008), antimicrobial (Xia et al 2011), antioxidant (Xia et al 2010), osteoporosis inhibition (Yan et al 2014) (Sheo, Lee, Chung 1990) and the promotion of alcohol metabolism (Hwang, Ham, Nam 2004).

Melanin acts like a filter in a living body, absorbing or scattering ultraviolet rays, thereby preventing skin tissue from being damaged by ultraviolet rays (Kim, 2009). However, a large amount of melanin causes aesthetic problems such as melasma. Melanin is stimulated by ultraviolet light and melanocyte stimulating hormone (MSH) (Parvez et al 2006). In the melanogenesis, tyrosine is converted to 3,4-dihydroxyphenylalanine (DOPA) by the catalytic reaction of tyrosinase enzyme, and DOPA is again DOPAquinone by tyrosinase. DOPAquinone is then eumelanin via DOPAchrome (Parvez et al 2006).

Here tyrosinase is a melanin biosynthetic enzyme and is a general inhibitory target of whitening agents (Ando et al 2007). The whitening agents that inhibit tyrosinase include hydroquinone, azelaic acid, kojic acid, arbutin, N-acetyl-4-S-cysteaminylphenol, aloesin, licorice extract licorice extract, vitamin E, and the like.

There are niacinamide, lectin, and neoglycoprotein, which block the movement of melanosome into keratinocytes. The whitening agents that accelerate the keratinization cycle of epidermal cells and have a whitening effect include α-hydroxyacid (AHA), salicylic acid, and retinoid (Ebanks, Wickett, Boissy 2009). However, hydroquinone, one of the most commonly used whitening ingredients in the world, has a problem in safety and new whitening ingredients have been studied to replace it (Parvez et al 2006).

Korean Patent Publication No. 10-2010-0059347 Korean Patent Laid-Open No. 10-2012-0113459

Another object of the present invention is to provide a method for producing sulfuric acid seed essential oil.

It is another object of the present invention to provide a whitening cosmetic composition comprising sulfur matel seed essential oil produced by the above production method.

In order to achieve the above object,

The present invention relates to: 1) a step of removing seeds by removing sulfur mulch pulp, removing the hard bark of the seeds, followed by naturally drying and pulverizing (step 1); And

2) After the pulverization, the deionized water and the pulverized sulfurized plum seeds were mixed and heated at 90 to 130 ° C for 2 to 4 hours by water distillation, and then the mixture was stirred at room temperature to remove water, A step of producing plum seed essential oil (step 2);

To provide a method for producing sulfur plum seed essential oil.

In addition, the present invention provides a whitening cosmetic composition comprising the sulfur-plum seed essential oil produced according to the above-described production method.

The sulfuric acid seed essential oil according to the present invention has an effect of inhibiting tyrosinase activity, inhibiting the production of melanin, and improving the pigment deposition, which is useful as a skin whitening cosmetic composition have.

Fig. 1 is a graph showing mushroom tyrosinase inhibitory effect of sulfur seed seed essential oil.
Fig. 2 is a graph showing mushroom tyrosinase inhibitory effect of sulfuric plum seed extract and sulfurized plum seed essential oil (essential concentration 0.01%).
3 is a graph showing cell-free tyrosinase inhibitory activity of sulfuric acid seed essential oil (essential oil).
FIG. 4 is a graph showing the inhibitory effect on the melanin biosynthesis of sulfuric acid seed essential oil (essential oil) in B16F10 cells. FIG.
Fig. 5 is a comparison chart of melanin biosynthesis inhibiting effect of sulfur mese seed extract and sulfurized plum seed essential oil (essential oil) in B16F10 cells (treatment concentration 0.01%).
6 is a graph showing the survival rate of B16F10 cells against sulfuric acid seed essential oil (essential oil).

Hereinafter, the present invention will be described in detail.

How to make sulfur plum seed essential oil

The present invention relates to a method for producing a seed of the present invention, comprising the steps of: 1) separating seeds by removing sulfur mulch pulp, removing the hard shell of the seeds, and then naturally drying and pulverizing (step 1); And

2) After the pulverization, the deionized water and the pulverized sulfurized plum seeds were mixed and heated at 90 to 130 ° C for 2 to 4 hours by water distillation, and then the mixture was stirred at room temperature to remove water, A step of producing plum seed essential oil (step 2);

To provide a method for producing sulfur plum seed essential oil.

In the manufacturing method according to the present invention, the step 2 may mix 300 to 700 parts by weight of deionized water with 100 parts by weight of pulverized sulfur plum seed, preferably 100 parts by weight of ground grass plum seed with deionized water 400 to 600 parts by weight, and more preferably 100 parts by weight of pulverized sulfur mats seed may be mixed with 500 parts by weight of deionized water.

Cosmetic composition for whitening skin

The present invention provides a cosmetic composition for skin whitening comprising the sulfuric acid seed essential oil.

The term " whitening " of the present invention refers to inhibiting, inhibiting or alleviating hyperpigmentation of the skin. Hyperpigmentation of the skin includes freckles, stains, hyperpigmentation after exposure to ultraviolet light, hyperpigmentation after inflammation, aging black spots, brown spots or black spots.

In the cosmetic composition according to the present invention, the skin whitening cosmetic composition may contain 0.0001 to 90 parts by weight of the total amount of the sulfuric acid seed essential oil, but if it contains an effective amount capable of providing a desired whitening effect But is not limited thereto.

The term " effective amount " of the present invention means the amount of a compound capable of inhibiting, inhibiting or alleviating hyperpigmentation of the skin. The effective amount of the sulfuric acid seed essential oil contained in the skin whitening composition of the present invention will vary depending on the form in which the composition for skin whitening is commercialized, the method in which the sulfuric acid seed essential oil is applied to the skin, and the time on the skin. For example, when the composition for skin whitening is commercialized as a medicament for dermatological treatment due to hyperpigmentation of skin, it is preferable to use the above-mentioned sulfur plum seed essential oil at a higher concentration than a cosmetic product which is routinely applied to skin. . In the case of wash-off type cosmetics such as a make-up remover, a detergent and the like in which the active ingredient remains on the skin in a short period of time even when the product is made into a cosmetic product, it may contain a relatively high concentration of the sulfur- will be. On the other hand, in the case of leave-on type cosmetics such as lotion, cream, essence and the like in which the active ingredient remains on the skin for a long period of time, a lower concentration of the sulfur plum seed essential oil than that of the wash- It may be included.

The cosmetic composition for skin whitening according to the present invention can be used for skin whitening, cream, softening longevity, nutritional lotion, pack, essence, hair tonic, shampoo, rinse, hair conditioner, hair treatment, gel, skin lotion, Lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser , ≪ / RTI > and the like. The composition of each of these formulations may contain various kinds of bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

The composition of the present invention may contain at least one skin whitening active ingredient which exhibits the same or similar function in addition to the sulfur matel seed essential oil of the present invention. Skin whitening active ingredients include kojic acid and its derivatives, arbutin, ascorbic acid and its derivatives, hydroquinone and its derivatives, resorcinol, cycloalkanone, methylenedioxyphenylalkanol, 2,7-dinitroindazole or Plant extracts such as mandarin orange extract, rice extract, licorice extract, and the like, but are not limited thereto.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The formulation of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a humidifier, a fragrance, a fragrance carrier, a protein, a solubilizing agent, a sugar derivative, a sunscreen, a vitamin, .

When the cosmetic composition for skin whitening as described above is formulated into pharmaceuticals or cosmetics, it may contain known excipients applicable to the skin acting as a carrier for the active ingredient. When formulating into pharmaceuticals, reference is made to the disclosure of [Remington ' s Pharmaceutical Science, Mack Publishing Company, Easton PA], and in formulation into cosmetics, International cosmetic ingredient dictionary, 6th ed., The cosmetic, Toiletry and Fragrance Association, Inc., Washington, 1995). Which are incorporated herein by reference.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples.

<Preparation Example 1> Reagents and cells

L-DOPA, phenylmethanesulfonyl fluoride (PMSF), 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT), kojic acid tyrosinase, α-melanocyte stimulating hormone acid was purchased from Sigma-Aldrich (USA), sodium sulfate was purchased from MBcell (USA), dimethyl sulfoxide (DMSO), Triton X-100 from Amresco (USA) and Bradford solution from Bio-Rad (USA).

Mouse melanocyte B16F10 cells were purchased from ATCC (USA) and cultured in Dulbecco's Modified Eagle Medium (DMEM; Gibco, USA) containing 10% fetal bovine serum (FBS; Gibco, USA).

Example 1 Preparation of Oil of Sulfuric Acid Seed Extract

The sperm yarn was collected from Daegu City in June, 2016. The seeds were removed by removing the sperm pulp, and then the hard shell of the seed was removed, followed by natural drying and pulverization

300 g of the pulverized sulfuric plum seeds were placed in a round flask, and 1.5 L of deionized water was added thereto. The resulting mixture was put into a Clevenger type apparatus (Gohung Chezja, Korea) by water distillation and heated at 100 ° C for 3 hours.

Upon heating, the evaporated essential oil was cooled through a cooling tube to collect the condensed essential oil.

Sodium sulfate (0.1 g) was added to the collected essential oil, and the mixture was stirred at room temperature to remove water. Then, the syringe was filtered with a syringe filter (0.2 μm, Millipore) to remove impurities.

The sulfur plum seed essential oil was placed in a glass container, shaded and stored at 4 ° C.

The yield of sulfur plum seed essential oil was 0.38% (w / w) and the density was 1.0 g / ml.

&Lt; Comparative Example 1 &

The dried sulfur plum seeds were pulverized and 10 g of pulverized sulfur plum seeds were added to 100 mL of deionized water and extracted at 60 DEG C for 24 hours. After centrifugation at 10,000 × g for 10 minutes, the supernatant was taken out and filtered with a filter paper (No. 2, Whatman, UK) to prepare Comparative Example 1.

&Lt; Comparative Example 2 &

Comparative Example 1 was lyophilized with a lyophilizer (Ilshin Lab, Korea), and 100 mL of deionized water was re-dissolved to prepare Comparative Example 2.

&Lt; Comparative Example 3 &

The dried sulfur plum seeds were pulverized and 10 g of ground pulverized plum seeds were added to 100 mL of an aqueous solution of 50% ethanol (Duksan Pharmaceutical, Korea) and extracted at 60 ° C for 24 hours. This was centrifuged at 10,000 × g for 10 minutes, and the supernatant was taken out and filtered with a filter paper (No. 2, Whatman, UK) to prepare Comparative Example 3.

&Lt; Comparative Example 4 &

The dried sulfur plum seeds were pulverized and 10 g of ground pulverized plum seeds were added to 100 mL of 100% ethanol (Duksan Chemical Co., Korea) and extracted at 60 DEG C for 24 hours. This was centrifuged at 10,000 × g for 10 minutes, and the supernatant was taken out and filtered with a filter paper (No. 2, Whatman, UK) to prepare Comparative Example 4.

&Lt; Comparative Example 5 &

The dried sulfur plum seeds were pulverized and 10 g of ground pulverized plum seeds were added to 100 ml of 20% 1,3-butylene glycol (Kosnet, Korea) and extracted at 60 ° C for 24 hours. This was centrifuged at 10,000 × g for 10 minutes, and the supernatant was taken out and filtered with a filter paper (No. 2, Whatman, UK) to prepare Comparative Example 5.

&Lt; Comparative Example 6 >

The dried sulfur plum seeds were pulverized and 10 g of ground pulverized plum seeds were added to 100 mL of 100% 1,3-butylene glycol (COSNET, Korea) and extracted at 60 DEG C for 24 hours. This was centrifuged at 10,000 × g for 10 minutes, and the supernatant was taken out and filtered with a filter paper (No. 2, Whatman, UK) to prepare Comparative Example 6.

<Experimental Example 1> Inhibitory effect of mushroom tyrosinase (Mushroom tyrosinase) activity

To examine the inhibitory effect of the mushroom type tyrosinase activity of Example 1 and Comparative Examples 1 to 6, 60 μL of 0.1 M potassium phosphate buffer (pH 6.8) and dimethyl sulfoxide (DMSO) were added to each well of a 1 μ microplate, 10 μL of the diluted sample was added. After adding 30 μL of 333 unit / mL mushroom tyrosinase solution, the mixture was left at room temperature for 5 minutes. 100 μL of a 12 mM L-DOPA solution was added thereto (Jeung Hyeon-Hyun, Minyuhong 2014). The reaction solution was measured for absorbance at 492 nm for 2 minutes using a microplate reader (Tecan, Switzerland) and calculated by equation (a) to calculate the inhibition rate of tyrosinase (Jung Hyun, Minhyuk).

(A)

Tyrosinase inhibition activity (%) = {(A-B) / A} 100

 A: Absent sample (control group) Absorbance

 B: absorbance when sample is contained

FIG. 1 shows mushroom tyrosinase inhibitory effect of sulfuric plum seed essential oil. FIG. 2 shows mushroom tyrosinase inhibition of the sulfur plum seed extract (Comparative Examples 1 to 6) and sulfur plum seed essential oil (Example 1) at a concentration of 0.01% Effect.

As a result, the sulfuric plum seed essential oil inhibited the activity of mushroom tyrosinase in a concentration-dependent manner, and the sulfuric acid seed essential oil (Example 1) had a very high inhibition rate of 95.5% as compared to the sulfuric acid seed extract of Comparative Examples 1 to 6 Respectively.

<Experimental Example 2> Cell-free tyrosinase inhibitory activity

The tyrosinase inhibitory activity of mouse melanocyte was measured as follows (Jeonghyun Jeong, Minhuhong 2014). Mouse melanocyte B16F10 cells were cultured in a 100-mm dish without any treatment, washed twice with phosphate buffered saline (PBS), and resuspended in 1% Triton X-100 and 1 mM PMSF in 0.1 M potassium phosphate buffer (pH 6.8) was added and homogenization was performed 30 times with Dounce homogenizer. This was centrifuged at 15,000 rpm for 15 min at 4 ° C and the supernatant was taken and the protein concentration was measured using a Bradford solution. To the new tube, 0.1 M potassium phosphate buffer (pH 6.8), sample, supernatant equivalent to 500 μg of protein, and 2.5 mM L-DOPA were added to a final volume of 1 mL. At this time, DMSO was added to dissolve the sample so that the final concentration became 0.1%. After incubation at 37 ° C for 15 minutes, the absorbance was measured at 475 nm. Tyrosinase inhibition rates were calculated using equation (a). Kojic acid was used as a control.

FIG. 3 shows cell-free tyrosinase inhibitory activity of sulfuric acid seed essential oil (essential oil). As a result, the sulfurized plum seed essential oil inhibited tyrosinase of mouse melanocyte B16F10 cells in a concentration - dependent manner. And showed almost the same inhibitory activity as kojic acid as a control group.

Experimental Example 3 Melanin Biosynthesis Inhibitory Activity

Inhibition of melanin biosynthesis in mouse melanocyte was measured as follows (Jung Hyun Jeong, Min Yoo Hong 2014). Mouse melanocyte B16-F10 cells were plated in a 6-well plate in an amount of 2 × 10 ⁵ . After 24 hours of culture, the medium was replaced with a medium containing 0.1% DMSO and a sample. At this time, α-MSH was added to the medium so as to have a concentration of 10 nM. After incubation for 2 days, the cells were washed twice with PBS, and 0.5 mL of lysis buffer (20 mM Tris, 0.1% Triton X-100, pH 7.6) was added. The cells were scraped and left at 4 ° C for 1 hour. And then centrifuged at 12,000 x g for 5 minutes at 4 ° C. 200 [mu] L of 1N NaOH was added to the resulting pellet and allowed to stand at 60 DEG C for 1 hour to dissolve. The absorbance was measured at 405 nm with a microplate reader (Tecan, Switzerland). The amount of melanin production was expressed as% compared with the absorbance of the group not treated with? -MSH. Kojic acid was used as a control.

FIG. 4 shows melanin biosynthesis inhibitory effect of the sulfuric acid seed essential oil (essential oil) in B16F10 cells. FIG. 5 shows the effect of the sulfur mica seed extract (Comparative Examples 1 to 6) and sulfur plum And inhibited the melanin biosynthesis of seed essential oil (Example 1).

 As a result, it was confirmed that melanin biosynthesis was reduced by reducing melanin content of mouse melanocyte B16F10 cells up to 0.01%, and melanin biosynthesis inhibition effect was increased from 0.005% to kojic acid than control group. Therefore, it was confirmed that the essential oil of the sulfur plum seeds has a very high inhibitory effect on melanin biosynthesis.

In addition, when treated at a concentration of 0.01%, the sulfuric acid seed essential oil showed higher inhibitory activity than the sulfuric acid seed extracts of Comparative Examples 1 to 6.

&Lt; Experimental Example 4 >

Toxicity MTT assay was performed on mouse melanocyte B16F10 cells as follows (Jeonghyun Jeong and Minhuhong 2014). B16F10 cells were plated in 96-well plates at 5 × 10 ³ cells / well and cultured for 24 hours. After that, the medium was replaced with fresh medium containing 0.1% DMSO and samples for each concentration, and cultured for 24 hours. Then, 20 μL of 5 mg / mL MTT was added to each well and further incubated for 3 hours. The medium was removed and 100 μL of DMSO was added to dissolve the crystal. The absorbance at 570 nm was measured using a microplate reader. Cell viability was calculated using equation (b). Kojic acid was used for comparison.

(B)

Cell survival rate (%) = (A / B) x 100

A: Absorbance of the cell reactant treated with the sample

B: Absorbance of the cell reaction without sample treatment

Figure 6 shows the survival rate of B16F10 cells against sulfuric acid seed essential oil (essential oil). As a result, the sulfuric acid seed essential oil showed no toxicity to B16F10 cells at a treatment concentration of 0.01% or more and a survival rate of 100% or more. And this was higher than the control group kojic acid.

Production Example of Cosmetic

According to the present invention, the sulfur plum seed extract or the sulfur plum seed essential oil can be manufactured into various types of cosmetics according to the purpose. Hereinafter, the present invention is not limited to the method for producing some cosmetics containing the sulfur matel seed extract or the sulfur matel seed essential oil as an active ingredient according to the present invention.

&Lt; Cosmetic Production Example 1 >

Safflower seed essential oil 10.0 parts by weight

1,3-butylene glycol 1.00 parts by weight

Disodium iodide 0.05 part by weight

Allantoin 0.10 parts by weight

Dipotassium glycyrrhizate 0.05 part by weight

Citric acid 0.01 part by weight

Sodium citrate 0.02 parts by weight

Glycereth-26 1.00 parts by weight

Arbutin 2.00 parts by weight

Hydrogenated castor oil 1.00 parts by weight

ethanol 30.0 parts by weight

Preservative a very small amount

coloring agent a very small amount

Flavoring agent a very small amount

Purified water Balance

<Cosmetic Preparation Example 2> Preparation of nutritional cream

Safflower seed essential oil 10.0 parts by weight

1,3-butylene glycol 7.00 parts by weight

glycerin 1.00 parts by weight

D-Panthenol 0.10 parts by weight

Plant extract 3.20 parts by weight

Magnesium aluminum silicate 0.30 parts by weight

PEG-40 stearate 1.20 parts by weight

Stearic acid 2.00 parts by weight

Polysorbate 60 1.50 parts by weight

Chin type glyceryl stearate 2.00 parts by weight

Sorbitan sesquioleate 1.50 parts by weight

Cetearyl alcohol 3.00 parts by weight

Mineral oil 4.00 parts by weight

Squalane 3.80 parts by weight

Carlyric / capric triglyceride 2.80 parts by weight

vegetable oil 1.80 parts by weight

Dimethicone 0.40 parts by weight

Deposium glycyrrhizae a very small amount

Allantoin a very small amount

Sodium hyaruronate a very small amount

Tocopheryl acetate Suitable amount

Triethanolamine Suitable amount

Preservative Suitable amount

Flavoring agent Suitable amount

Purified water Balance

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (5)

1) separating the seeds by removing the dried plum flesh, removing the hard bark of the seeds, followed by natural drying and pulverizing (step 1); And
2) After the pulverization, the deionized water and the pulverized sulfurized plum seeds were mixed and heated at 90 to 130 ° C for 2 to 4 hours by water distillation, and then the mixture was stirred at room temperature to remove water, A step of producing plum seed essential oil (step 2);
&Lt; / RTI &gt; by weight of the essential oil. The method according to claim 1,
Wherein the step (2) comprises mixing 300 to 700 parts by weight of deionized water with 100 parts by weight of pulverized sulfur plum seeds. The method according to claim 1,
Wherein the step (2) comprises mixing 100 parts by weight of pulverized sulfur-plum seed with 400 to 600 parts by weight of deionized water. A whitening cosmetic composition comprising an essential oil prepared by the manufacturing method of claim 1. 5. The method of claim 4,
The composition may be used as an external ointment for skin, cream, soft longevity, nutritional lotion, pack, essence, hair tonic, shampoo, rinse, hair conditioner, hair treatment, gel, skin lotion, , Moisturizing lotion, nutritional lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser &Lt; / RTI &gt; wherein the composition is prepared in one formulation.

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