KR102015176B1 - Japanese apricot essential oil and cosmetic composition for skin whitening containing the same - Google Patents

Abstract

The present invention relates to a yellow plum seed essential oil and a whitening cosmetic composition comprising the same, yellow plum seed essential oil has an effect of inhibiting tyrosinase activity, inhibiting the production of melanin, resulting pigmentation There is an effect to improve the skin whitening may be useful as a cosmetic composition.

Description

Japanese apricot essential oil and cosmetic composition for skin whitening containing the same}

The present invention relates to a yellow plum seed essential oil and a whitening cosmetic composition comprising the same.

Prunus mume Sieb. Et Zucc, a genus of Rosaceae, is native to China's Hubei and Sichuan provinces, and has been cultivated for about 3000 years in Korea and Japan. In China, the fruit, flowers, branches, leaves, roots, etc. of the plum tree are used as medicine. In particular, the fruit of the plum tree is called plum, and has been used as an edible or medicinal herb for plum wine and pickles since ancient times. Plums are 2 ~ 3cm in diameter, have a lot of hair on the skin, and have a strong sour taste (Lee Young-eun, Hong Seung-heon 2004). Nucleus (核果) is a nucleus (核,), green, or in June-July ripening turns yellow. Plums are divided into five types according to harvest time and processing method (Lee Young-eun, Hong Seung-heon 2004). Cheongmae (靑 梅) is a less ripe green plum harvested in May-June and has a strong sour taste and hard flesh. Huangmae (黃梅) is yellow and harvested in June-July. Geummae (청 梅) is steamed and dried Cheongmae, Omae (烏梅) is a plum made by fumigation the black plum. White plum (白梅) is a pickled marinated in brine and dried. Cheongmae has a clearing glycoside, amygdalin (amygdalin) is a dangerous to reproduce because the human ingestion is produced by the digestive enzymes (hydrogen cyanide, HCN) by intestinal digestion.

Plum is used as a medicinal herb in oriental medicine. It is good for detoxification, antipyretic, thirst quenching, and cough relieving and when it is indigestible (Lee Young Eun, Hong Seung Heon 2004). Plum has a cosmetic effect to promote parotin secretion of the parotid gland to make the face and skin shine (Joo Young-seung, Kim Ki-yeon, Jang Sung-hwan 2004). Scientific effects of plum include: influenza A virus suppression (Yingsakmongkon et al 2008), antibacterial (Xia et al 2011), antioxidant (Xia et al 2010), osteoporosis suppression (Yan et al 2014), anticancer (Jeong et al 2006), It is diverse from healthy stomach (Sheo, Lee, Chung 1990) and alcohol metabolism promotion (Hwang, Ham, Nam 2004).

Melanin acts like a filter of a living body and absorbs or scatters ultraviolet rays to prevent skin tissue from being damaged by ultraviolet rays (Kim Choonja 2009). However, higher amounts of melanin cause aesthetic problems such as melasma. Melanin is promoted by ultraviolet rays and melanocyte stimualting hormone (MSH) and the like (Parvez et al 2006). The mechanism of melanin production is that tyrosine becomes 3,4-dihydroxyphenylalanine (DOPA) by catalysis of tyrosinase enzyme and DOPA becomes DOPAquinone by tyrosinase. DOPAquinone then becomes eumelanin via DOPAchrome (Parvez et al 2006).

Tyrosinase is a melanin biosynthesis enzyme that is the target of general inhibition of whitening agents (Ando et al 2007). Whitening ingredients that inhibit tyrosinase include hydroquinone, azelaic acid, kojic acid, arbutin, N-acetyl-4-S-cysteaminylphenol, aloesine and licorice extract (licorice extract) and vitamin E.

Whitening ingredients that prevent the transfer of melanocytes to keratinocytes include niacinamide, lectin, neoglycoprotein, etc.Also, α-hydroxyacid accelerates the keratinization cycle of epidermal cells. (AHA), salicylic acid and retinoid (Ebanks, Wickett, Boissy 2009). However, in the case of hydroquinone, one of the most used whitening ingredients in the world, there is a safety problem, and new whitening ingredients are being researched to replace it (Parvez et al 2006).

Korean Patent Publication No. 10-2010-0059347 Korean Patent Publication No. 10-2012-0113459

It is another object of the present invention to provide a method for preparing sulfur plum seed essential oil.

It is another object of the present invention to provide a whitening cosmetic composition comprising the yellow plum seed essential oil prepared by the above method.

In order to achieve the above object,

The present invention 1) removing the yellow plum fruit flesh to separate the seeds, removing the hard shell of the seed, and then dried by natural drying (step 1); And

2) After the pulverization, deionized water and pulverized sulfur plum seeds were mixed and heated to 90 to 130 ° C. for 2 to 4 hours by water distillation, and then stirred at room temperature to remove water, and filtered to obtain sulfur. Preparing plum seed essential oil (step 2);

It provides a method for producing a seed oil essential oil yellow plum.

In addition, the present invention provides a whitening cosmetic composition comprising a yellow plum seed essential oil prepared according to the above production method.

Hwangmaesil seed essential oil according to the present invention is effective in inhibiting tyrosinase activity, inhibiting the production of melanin, and improving the resulting pigmentation, which may be useful as a cosmetic composition for skin whitening. have.

1 is a diagram showing the mushroom tyrosinase inhibitory effect of the seed oil essential oil yellow plum.
Figure 2 is a diagram showing the mushroom tyrosinase inhibitory effect of yellow plum seed extract and yellow plum seed essential oil (essential oil) (treatment concentration 0.01%).
Figure 3 is a diagram showing the cell-free tyrosinase inhibitory activity of yellow plum seed essential oil (essential oil).
Figure 4 is a diagram showing the inhibitory effect of melanin biosynthesis of yellow plum seed essential oil (essential oil) in B16F10 cells.
5 is a diagram comparing the melanin biosynthesis inhibitory effect of yellow plum seed extract and yellow plum seed essential oil (essential oil) in B16F10 cells (treatment concentration 0.01%).
Figure 6 is a diagram showing the survival rate of B16F10 cells for essential oil seed oil (yellow oil).

Hereinafter, the present invention will be described in detail.

Hwangmaesil Seed Essential Oil Manufacturing Method

The present invention is 1) removing the yellow plum fruit flesh to separate the seed, and then removing the hard shell of the seed and then dried by natural drying (step 1); And

2) After the pulverization, deionized water and pulverized sulfur plum seeds were mixed and heated to 90 to 130 ° C. for 2 to 4 hours by water distillation, and then stirred at room temperature to remove water, and filtered to obtain sulfur. Preparing plum seed essential oil (step 2);

It provides a method for producing a seed oil essential oil yellow plum.

In the manufacturing method according to the present invention, the step 2 may be mixed with deionized water 300 to 700 parts by weight to 100 parts by weight of the crushed yellow plum seeds, preferably deionized water to 100 parts by weight of crushed yellow plum seeds 400 to 600 parts by weight may be mixed, and more preferably, 500 parts by weight of deionized water may be used in 100 parts by weight of crushed yellow plum seeds.

Cosmetic composition for skin whitening

The present invention provides a cosmetic composition for skin whitening comprising the yellow plum seed essential oil.

As used herein, the term "whitening" refers to inhibiting, inhibiting or alleviating hyperpigmentation of the skin. Hyperpigmentation of the skin includes freckles, blemishes, hyperpigmentation after UV exposure, hyperpigmentation after inflammation, senile black spots, brown spots or blotch.

In the cosmetic composition according to the present invention, the cosmetic composition for skin whitening may be contained in an amount of 0.0001 to 90 parts by weight based on the total weight of the yellow plum seed essential oil, provided that it includes an effective amount that can provide a desirable whitening effect. This is not restrictive.

As used herein, the term "effective amount" means the amount of a compound that can inhibit, inhibit or alleviate hyperpigmentation of the skin. The effective amount of the yellow plum seed essential oil included in the skin whitening composition of the present invention will vary depending on the form in which the skin whitening composition is commercialized, the method of applying the yellow plum seed essential oil to the skin, and the time to stay in the skin. For example, when the composition for skin whitening is commercialized as a medicament for dermatological treatment due to hyperpigmentation of the skin, the yellow plum seed essential oil is used at a higher concentration than when it is commercialized to cosmetics that are applied to the skin on a daily basis. It may include. Even in the case of cosmetics, wash-off type cosmetics such as makeup remover and detergent, in which the active ingredient stays on the skin in a short time, may contain a relatively high concentration of the yellow plum seed essential oil. will be. On the other hand, in the case of leave-on type cosmetics such as lotion, emulsion, cream, essence, etc., in which the active ingredient stays on the skin for a long time, the concentration of the yellow plum seed essential oil is lower than that of wash-off type cosmetics. It may be included.

Cosmetic composition for skin whitening according to the present invention is a skin external ointment, cream, supple cosmetics, nourishing cosmetics, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner , Astringent, Lotion, Milk Lotion, Moisture Lotion, Nutrition Lotion, Massage Cream, Nutrition Cream, Moisture Cream, Hand Cream, Foundation, Nutrition Essence, Sunscreen, Soap, Cleansing Foam, Cleansing Lotion, Cleansing Cream, Body Lotion and Body Cleanser It may have a formulation selected from the group consisting of, but is not limited thereto. The composition of each of these formulations may contain a variety of bases and additives necessary for the formulation of the formulation and are suitable, and the types and amounts of these components can be readily selected by those skilled in the art.

The composition of the present invention may contain one or more skin lightening active ingredients that exhibit the same or similar function in addition to the yellow plum seed essential oil of the present invention. Skin whitening active ingredients include kojic acid and derivatives thereof, arbutin, ascorbic acid and derivatives thereof, hydroquinone and derivatives thereof, resorcinol, cycloalkanone, methylenedioxyphenyl alkanol, 2,7-dinitroindazole or Plant extracts such as vine tangerine extract, rice extract, licorice extract, and the like, but is not limited thereto.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Formulations of the present invention may further contain excipients, including fluorescent substances, fungicides, hydrogenic agents, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like. .

When the cosmetic composition for skin whitening is formulated as a medicine or cosmetic as described above, it may include a known excipient applicable to the skin to act as a carrier for the active ingredient. When formulated as a drug, reference may be made to the contents disclosed in [Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA], and when formulated as a cosmetic, [International cosmetic ingredient dictionary, 6th ed., The cosmetic, Toiletry] and Fragrance Association, Inc., Washington, 1995]. Such documents are incorporated as part of this specification.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are merely to illustrate the present invention, but the content of the present invention is not limited by the following examples.

Preparation Example 1 Reagents and Cells

Mushroom tyrosinase, α-melanocyte stimulating hormone (α-MSH), L-DOPA, phenylmethanesulfonyl fluoride (PMSF), 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT), kojic Acid was purchased from Sigma-Aldrich (USA), sodium sulfate from MBcell (USA), dimethyl sulfoxide (DMSO), Triton X-100 from Amresco (USA), and Bradford solution from Bio-Rad (USA).

B16F10 cells, mouse melanocytes, were cultured in ATCC (USA) and cultured with Dulbecco Modified Eagle Medium (DMEM; Gibco, USA) containing 10% fetal bovine serum (FBS; Gibco, USA).

Example 1 Preparation of Yellow Plum Seed Extract Oil

The yellow plum was used in Daegu city in June 2016, and the seeds were separated by removing the yellow plum fruit flesh, and then the hard shell of the seed was removed and then dried by natural drying.

300 g of the pulverized yellow plum seeds were placed in a round flask, mixed with 1.5 L of deionized water, and then mixed in a Clevenger type apparatus (Goheungcho, Korea) by water distillation and heated at 100 ° C. for 3 hours.

The condensed essential oils were collected by cooling the essential oil evaporated upon heating through a cooling tube.

0.1 g of sodium sulfate was added to the collected essential oil, stirred at room temperature to remove water, and then filtered through a syringe filter (0.2 μm, Millipore) to remove impurities to prepare a sulfur plum seed essential oil.

The yellow plum seed essential oil was placed in a glass container and shaded and stored at 4 ° C.

Yield of the yellow plum seed essential oil was 0.38% (w / w) and the density was 1.0 g / ml.

Comparative Example 1

The dried yellow plum seeds were ground, and 10 g of the ground yellow plum seeds were placed in 100 mL of deionized water and extracted at 60 ° C. for 24 hours. After centrifugation at 10,000 × g for 10 minutes, the supernatant was taken and filtered with filter paper (No. 2, Whatman, UK) to prepare Comparative Example 1.

Comparative Example 2

After Comparative Example 1 was lyophilized with a freeze-dryer (Ilsin Lab, Korea), 100 mL of deionized water was added again to dissolve, thereby preparing Comparative Example 2.

Comparative Example 3

The dried yellow plum seeds were pulverized, and 10 g of the crushed yellow plum seeds were placed in 100 mL of 50% ethanol (Duksan Pharm. Korea) solution and extracted at 60 ° C. for 24 hours. After centrifugation at 10,000 × g for 10 minutes, the supernatant was taken and filtered with filter paper (No. 2, Whatman, UK) to prepare Comparative Example 3.

<Comparative Example 4>

The dried yellow plum seeds were pulverized, and 10 g of the pulverized yellow plum seeds were placed in 100 mL of 100% ethanol (Duksan Pharm., Korea) and extracted at 60 ° C. for 24 hours. After centrifugation at 10,000 × g for 10 minutes, the supernatant was taken and filtered with filter paper (No. 2, Whatman, UK) to prepare Comparative Example 4.

Comparative Example 5

The dried yellow plum seeds were ground, and 10 g of the ground yellow plum seeds were placed in 100 mL of 20% 1,3-butylene glycol (Cosnet, Korea) and extracted at 60 ° C for 24 hours. After centrifugation at 10,000 × g for 10 minutes, the supernatant was taken and filtered with filter paper (No. 2, Whatman, UK) to prepare Comparative Example 5.

Comparative Example 6

The dried yellow plum seeds were ground, and 10 g of the ground yellow plum seeds were placed in 100 mL of 100% 1,3-butylene glycol (Cosnet, Korea) and extracted at 60 ° C for 24 hours. After centrifugation at 10,000 × g for 10 minutes, the supernatant was taken and filtered with filter paper (No. 2, Whatman, UK) to prepare Comparative Example 6.

Experimental Example 1 Inhibitory Effect of Mushroom Tyrosinase Activity

In order to confirm the inhibitory effect of mushroom tyrosinase activity of Example 1 and Comparative Examples 1 to 6, 60 μL of 0.1 M potassium phosphate buffer (pH 6.8) and dimethyl sulfoxide (DMSO) in each well of the ll microplate. 10 μL of diluted sample was added. 30 μL of 333unit / mL mushroom tyrosinase solution was added thereto and allowed to stand at room temperature for 5 minutes. 100 μL of 12 mM L-DOPA solution was added thereto (Ahn Jung-hyun, Min Yu-hong 2014). The reaction solution was measured for 2 minutes at 492 nm using a microplate reader (Tecan, Switzerland) and calculated by Equation (a) to calculate the tyrosinase inhibition rate (Ahn, Hyun-Hoon, 2014).

Formula (a)

Tyrosinase inhibition activity (%) = {(A-B) / A} × 100

 A: Absorbance when no sample (control)

 B: absorbance when sample enters

1 shows mushroom tyrosinase inhibitory effect of yellow plum seed essential oil, and in FIG. 2, mushroom tyrosinase inhibition of yellow plum seed extract (Comparative Examples 1 to 6) and yellow plum seed essential oil (Example 1) at a concentration of 0.01%. The effect was shown.

As a result, the yellow plum seed essential oil inhibited the activity of mushroom tyrosinase in a concentration-dependent manner, and the yellow plum seed essential oil (Example 1) had a very high inhibition rate as compared to the yellow plum seed extracts of Comparative Examples 1-6. Indicated.

Experimental Example 2 Cell-free Tyrosinase Inhibitory Activity

Tyrosinase inhibitory activity of mouse melanocytes was measured as follows (Jung, Ahn, 2014). B16F10 cells, mouse melanocytes, were cultured in a 100 mm dish without any treatment and washed twice with phosphate buffered saline (PBS), and then 0.1 M potassium phosphate buffer at 4 ° C containing 1% Triton X-100 and 1 mM PMSF. (pH6.8) was added and stroked 30 times with a dounce homogenizer for homogenization. This was centrifuged at 4 ° C. for 15 minutes at 15,000 rpm, the supernatant was taken, and the protein concentration was measured using Bradford solution. In a new tube, 0.1M potassium phosphate buffer (pH6.8), a sample, a supernatant corresponding to 500 μg of protein, and 2.5mM L-DOPA were added so that the final volume was 1mL. At this time, DMSO was added to dissolve the sample so that the final concentration was 0.1%. And it was left for 15 minutes at 37 ℃ absorbance was measured at 475 nm. Tyrosinase inhibition rate was calculated using equation (a). Kojic acid was used as a control.

Figure 3 shows the cell-free tyrosinase inhibitory activity of the seed oil essential oil (essential oil). As a result, yellow plum seed essential oil inhibited the tyrosinase of B16F10 cells, which are mouse melanocytes, in a concentration-dependent manner. It showed almost similar inhibitory activity as the control kojic acid.

Experimental Example 3 Melanin Biosynthesis Inhibitory Activity

The inhibitory effect of melanin biosynthesis on mouse melanocytes was measured as follows (Ahn, Hyun-Hoon, 2014). B16-F10 cells, which are mouse melanocytes, were placed in a ⁶ well plate at an amount of 2 × 10 ⁵ . After 24 hours of incubation, the medium was replaced with a medium containing 0.1% DMSO and samples. At this time, α-MSH was added to the medium to 10 nM. After incubation for 2 days, the cells were washed twice with PBS, 0.5mL of lysis buffer (20mM Tris, 0.1% Triton X-100, pH 7.6) and scraped the cells and left for 1 hour at 4 ℃. Then centrifuged for 5 minutes at 12,000 × g at 4 ℃. 200 μL of 1N NaOH was added to the resulting pellet, and the mixture was left at 60 ° C. for 1 hour to be dissolved. Next, the absorbance was measured at 405 nm with a microplate reader (Tecan, Switzerland). Melanin production was expressed in% compared to the absorbance of the group not treated with α-MSH. Kojic acid was used as a control.

FIG. 4 shows melanin biosynthesis inhibitory effect of yellow plum seed essential oil (essential oil) in B16F10 cells, and yellow plum seed extract (Comparative Examples 1 to 6) and yellow plum in 0.01% treatment concentration in B16F10 cells. It showed the melanin biosynthesis inhibitory effect of seed essential oil (Example 1).

 As a result, it was confirmed that Hwangmaesil seed essential oil decreased melanin content by decreasing the melanin content of mouse melanocyte B16F10 cells in a concentration-dependent manner up to 0.01%, and the melanin biosynthesis inhibitory effect was higher than that of the control kojic acid from 0.005%. Therefore, it was confirmed that essential oil of yellow plum seeds had a very high melanin biosynthesis inhibitory effect.

In addition, when treated at a concentration of 0.01%, the yellow plum seed essential oil showed a higher inhibitory activity than the Comparative Examples 1 to 6 of the yellow plum seed extract.

Experimental Example 4 Cytotoxicity

Toxicity was measured as follows on M16 assay for B16F10 cells, mouse melanocytes (Jung, Ahn, 2014). B16F10 cells were placed in a 96-well plate at 5 × 10 ³ cells / well and incubated for 24 hours. Thereafter, the samples were exchanged with fresh medium containing 0.1% DMSO for each concentration and incubated for 24 hours. Then, 20 μL of 5mg / mL MTT was added to each well and incubated for 3 hours. After removing the medium, 100 μL of DMSO was added to dissolve the crystal, and the absorbance of 570 nm was measured using a microplate reader. Cell viability was calculated using equation (b). Kojic acid was used for comparison.

Formula (b)

Cell survival rate (%) = (A / B) × 100

A: absorbance of the cell reactants treated with the sample

B: absorbance of the cell reactants without sample treatment

Figure 6 shows the survival rate of B16F10 cells for the seed oil essential oil (essential oil). As a result, yellow plum seed essential oil showed no toxicity to B16F10 cells with a survival rate of 100% or more up to 0.01% treatment concentration. And this was higher survival than the control kojic acid.

Production example of cosmetics

The yellow plum seed extract or the yellow plum seed essential oil according to the present invention can be prepared in various forms according to the purpose. The following is an example of a method for preparing some cosmetics containing the yellow plum seed extract or the yellow plum seed essential oil as an active ingredient according to the present invention, but the present invention is not limited thereto.

Cosmetic Production Example 1 Preparation of Flexible Lotion

Yellow Plum Seed Essential Oil 10.0 parts by weight

1.00 parts by weight of 1,3-butylene glycol

0.05 parts by weight of disodium ethane

Allantoin 0.10 part by weight

0.05 parts by weight of dipotassium glycylizate

0.01 part by weight of citric acid

Sodium citrate 0.02 parts by weight

Glyceres-26 1.00 parts by weight

Arbutin 2.00 parts by weight

1.00 parts by weight of hydrogenated castor oil

Ethanol 30.0 parts by weight

Preservative Trace

Trace amount of colorant

Trace amount of flavor

Purified water level

Cosmetic Production Example 2 Preparation of Nutritious Cream

Yellow Plum Seed Essential Oil 10.0 parts by weight

1,3-butylene glycol 7.00 parts by weight

1.00 parts by weight of glycerin

0.10 part by weight of D-panthenol

3.20 parts by weight of plant extracts

0.30 parts by weight of magnesium aluminum silicate

PEG-40 stearate 1.20 parts by weight

2.00 parts by weight of stearic acid

Polysorbate 60 1.50 parts by weight

Lipid glyceryl stearate 2.00 parts by weight

1.50 parts by weight of sorbitan sesquioleate

Cetearyl Alcohol 3.00 parts by weight

4.00 parts by weight of mineral oil

3.80 parts by weight of squalane

Carlylic / capric triglycerides 2.80 parts by weight

1.80 parts by weight of vegetable oil

Dimethicone 0.40 part by weight

Diphosphium glycylizate traces

Allantoin Trace

Sodium Hyaluronate Trace

Tocopheryl acetate appropriate amount

Triethanolamine appropriate amount

Preservative

Flavoring Agent

Purified water level

So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown not in the above description but in particular in the claims, and all differences within the scope will be construed as being included in the present invention.

Claims (5)

1) removing the yellow plum fruit flesh to separate the seed, and then removing the hard shell of the seed and then dried by natural drying (step 1); And
2) After the pulverization, deionized water and pulverized sulfur plum seeds were mixed and heated to 100 to 130 ° C. for 2 to 4 hours by water distillation, and then stirred at room temperature to remove water, and filtered to obtain sulfur. Preparing plum seed essential oil (step 2);
Yellow plum seed essential oil manufacturing method comprising a. The method of claim 1,
Step 2 is characterized in that the deionized water is mixed with 300 to 700 parts by weight to 100 parts by weight of the crushed yellow plum seeds. The method of claim 1,
Step 2 is characterized in that the deionized water is mixed with 400 to 600 parts by weight to 100 parts by weight of the crushed yellow plum seeds. Whitening cosmetic composition comprising an essential oil prepared by the method of claim 1. The method of claim 4, wherein
The composition is an external skin ointment, cream, softening lotion, nourishing lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion , Moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser A composition characterized in that it is prepared in one formulation.

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