KR102394459B1 - A whitening composition comprising youngia sonchifolia extract - Google Patents

Abstract

The present invention relates to a whitening composition comprising the extract of Godeulbaegi as an active ingredient, and to a method for producing an extract of Godeulbaegi having excellent whitening effect. In particular, the present invention has excellent whitening effect and excellent antioxidant effect, as well as a long time used for food and medicine, so there is no problem related to tolerance or safety, so the whitening composition can be used for various purposes including whitening cosmetics. can be widely used as

Description

A whitening composition comprising the extract of fern extract as an active ingredient {A WHITENING COMPOSITION COMPRISING YOUNGIA SONCHIFOLIA EXTRACT}

The present invention relates to a whitening composition comprising a plant extract, specifically, an extract of Godeul-baegi as an active ingredient.

A person's skin color is determined by the concentration and distribution of melanin, keratin, and hemoglobin inside the skin. The factor that has the greatest influence on the skin color is melanin.

Melanin (melanin) is a representative pigment that represents the color of human skin, protects the skin, and plays an important role in preventing skin damage caused by ultraviolet (UV) rays. The melanin is produced by melanocyte cells in the basal layer of the epidermis of the skin. Melanin is produced through a non-enzymatic oxidation reaction after tyrosinase (EC: 1.14.18.1) acts on tyrosine, a kind of amino acid, to convert it into dopa and dopaquinone. The tyrosinase is one of a copper-containing monooxygenase containing a copper atom. As described above, it is an important enzyme in the melanin biosynthesis pathway. .

The melanin synthesized by the melanocyte cells is transferred to the surrounding keratinocytes to represent the color of human skin.

When the melanin is excessively produced in the skin, it darkens the skin color, causes spots, freckles, or spots, and causes hyperpigmentation, resulting in cosmetically undesirable results. Therefore, inhibiting the synthesis of melanin pigment in the skin not only brightens the skin color to realize skin whitening, but also improves skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones, and genetic causes.

Recently, as interest in skin increases along with an increase in interest in beauty and self-care, research on skin whitening is being actively conducted to remove skin blemishes such as spots and spots, and in another aspect, outdoor With the increase of the population enjoying leisure activities in the city, there is an increasing demand to prevent melanin pigmentation caused by UV rays.

The reduction in the biosynthesis of the melanin pigment may be performed by inhibiting tyrosinase, reducing exposure to ultraviolet (UV) light, or inhibiting melanocyte cells. Among the above methods, in relation to the existing cosmetic for whitening, a component capable of inhibiting tyrosinase was used as an active ingredient.

In relation to the above whitening cosmetics, conventionally, substances having inhibitory activity against tyrosinase such as hydroquinone, ascorbic acid, kojic acid, and glutathione, which are chemical components, are formulated in cosmetics such as ointments, creams, and essences for external use on the skin. By doing so, skin whitening was realized or skin hyperpigmentation such as spots and freckles was improved.

However, these ingredients are mainly synthetic materials, and safety issues are raised, or problems such as deterioration when used with cosmetics or in terms of efficacy.

Specifically, hydroquinone not only has a problem in that it has to be limited to a very small amount due to severe skin irritation, but also has recently raised issues such as allergy or skin cancer induction. It is also classified as a drug that requires a dermatologist's prescription, and it is regulated that a concentration of less than 1% of the total composition can be added only to external compositions sold in hospitals or pharmacies.

In addition, ascorbic acid, a vitamin C derivative, is easily oxidized, and cosmetics containing it have problems such as discoloration or discoloration, which lowers the quality of the product and thus has a disadvantage in that the whitening effect is lowered. Recently, derivatives such as ascorbyl-palmitate, ascorbyl-dipalmitate, ascorbyl stearare, or ascorbyl magnesium phosphate have been developed to solve this problem, but unless a special technique is used, it is difficult to penetrate into the skin.

In addition, kojic acid has the potential to be carcinogenic when administered orally, so further verification of safety is required. Thiol-based compounds such as glutathione and cysteine not only have a characteristic unpleasant odor, but also have a problem in transdermal absorption, and glycosides and derivatives thereof It also has a high polarity, making it difficult to use as a cosmetic ingredient.

In addition, substances having tyrosinase inhibitory activity isolated from existing natural materials mainly have a phenol structure or are a metal chelating agent, and the substances having inhibitory activity are low in activity. Nevertheless, its use is limited due to high toxicity and insufficient penetrative ability.

Therefore, there is an increasing need for research on ingredients capable of decomposing melanin itself, as an example of an active ingredient for whitening cosmetics that is more effective and safer than whitening agents so far.

KR 10-2004-0018733A KR 10-0527602B

The present invention is to improve the problems of the prior art as described above, and in particular, the present invention aims to provide an active ingredient and an optimal content of a skin whitening composition containing a natural substance that is effective for skin whitening and has almost no side effects. do.

In order to achieve the above object, the present invention provides a whitening composition comprising an extract ( Youngia sonchifolia ) as an active ingredient.

The extract of Godeulpagi may be preferably a hot water extract, more preferably a hot water extract of a freeze-dried product of Godeulbaegi.

The whitening composition may be used as a pharmaceutical composition having a whitening effect or a cosmetic having a whitening effect, and in this regard, it may be a skin external pharmaceutical composition for whitening or a cosmetic composition for whitening.

In this aspect, the present invention is an extract of Godeulbagi ( Youngia ) sonchifolia ) as an active ingredient, provides a pharmaceutical composition for external use for skin whitening. The extract of Godeulpagi may be preferably a hot water extract, more preferably a hot water extract of a freeze-dried product of Godeulpagi.

In another aspect, the present invention provides extracts from sonchifolia ) as an active ingredient, to provide a cosmetic composition for whitening.

The Godeulpagi extract is a hot water extract, preferably a hot water extract of the Godeulbaegi freeze-dried product, more preferably, dried Godeulpagi washed in running water at -20° C., followed by grinding to prepare a godeulbaegi milled product; And it may be prepared by a method comprising the step of adding water to the extract sample and extracting the extract with hot water at 80°C to 120°C.

In another aspect, the present invention provides a sample preparation step for preparing a godeulbagi extract by grinding the godeulbaegi washed in running water at -20°C, and then grinding to prepare a grinded plant; And it provides a method for producing an extract of Godeulbaegi having excellent whitening effect, comprising adding water to the extract sample and extracting it with hot water at 80°C to 120°C.

Unless otherwise specified herein, an active ingredient refers to a component that provides a whitening effect in a whitening composition, specifically, a pharmaceutical composition or a cosmetic composition.

In the present specification, unless otherwise specified, prevention means any action of suppressing the symptoms of a specific disease or delaying the progression by administration of the composition of the present invention.

Unless otherwise specified herein, treatment refers to any improvement action that improves or beneficially changes the symptoms of a specific disease by administration of the composition of the present invention.

Unless otherwise specified herein, an individual refers to all animals, such as humans, monkeys, dogs, goats, pigs, or mice, with diseases in which symptoms of specific diseases including gout can be improved by administering the composition of the present invention. .

Unless otherwise specified herein, administration means providing a given composition of the present invention to a subject by any suitable method.

Unless otherwise specified herein, pharmaceutically acceptable means that it is physiologically acceptable and does not normally cause allergic reactions such as gastrointestinal disorders, dizziness, or similar reactions when administered to animals, preferably humans. do.

While the inventors of the present invention were researching a material having whitening activity derived from a natural substance that does not have side effects or stability problems such as tolerance, the extract of Godeulbarae, which is excellent in whitening effect, specifically, tyrosinase inhibitory effect, among various plants has been It was confirmed that there was no safety problem, such as hydroquinone or kojic acid, which are ingredients used in the whitening active composition of Additional research was conducted on the whitening composition as an active ingredient, and in terms of the extraction solvent, the hot water extraction method using only an aqueous solvent as a solvent has the best whitening effect, In preparing the powder, it was confirmed that freeze-drying was the most excellent in the drying process of Godeul-baegi, and based on this, the present invention was completed.

Hereinafter, the present invention will be described in more detail.

The present invention is Godeulpagi ( Youngia sonchifolia ) relates to a whitening composition comprising an extract as an active ingredient. The Godeulpagi extract may be a hot water extract. The whitening composition may be used for pharmaceutical or cosmetic purposes.

In this aspect, the whitening composition may be a pharmaceutical composition for external use for skin whitening or a cosmetic composition for whitening.

The Godeulbaegi extract is a hot water extract, specifically, dried Godeulpagi washed in running water at -20°C, and then ground to prepare a Godeulbagi extract sample preparation step; And it may be prepared by a method comprising the step of adding water to the extract sample of Godeulbaegi and extracting it with hot water at 80°C to 120°C, preferably at 110°C.

The Godeulpagi ( Youngia ) sonchifolia ) is a dicotyledonous bicotyledonous plant belonging to the Asteraceae of the order Asteraceae, and is also called cinnamon or gochae. The stem is straight, branched, reddish-purple, the leaves are egg-shaped, the leaves growing from the root are hairless, the length is 2.3 to 6 cm, and the base is widened to surround the stem, and there are irregularly dented saw teeth. The size becomes smaller as it goes up, and the flowers are yellow and bloom from May to July. The capillary flowers hang at the end of the branches in corolla, the corolla is yellow and the ends are split, and the fruits are a black, flat cone-shaped fruit. ripen in the month

The Godeulbaegi is mainly distributed in the south central part of Korea, grows wild in the foothills of mountains and near fields or fields, and was also cultivated in farmhouses. The young leaves and roots of Godeul-ppaegi are made with kimchi or eaten as greens, and in folklore, the whole grass has been used as a medicine.

The extract may be a solvent extract extracted with an extraction solvent, a fraction obtained by adding a fractionation solvent to an extract prepared by extraction with an extraction solvent, or a purified product obtained by performing chromatography on the fraction.

The extraction solvent may be any one selected from the group consisting of water, an organic solvent, or a mixture thereof, preferably water, an alcohol having 1 to 5 carbon atoms, and mixtures thereof that can be used for extracting natural products. The organic solvent is a linear or branched alcohol having 1 to 5 carbon atoms such as methanol and ethanol, a polar solvent such as ethyl acetate or acetone, a non-polar solvent such as nucleic acid or dichloromethane, a neutral solvent such as a ketone having 3 to 5 carbon atoms, or these It may be a mixed solvent, preferably water, an alcohol having 1 to 5 carbon atoms or an aqueous alcohol solution, more preferably water.

The fractionation solvent may be water, an alkane having 5 to 7 carbon atoms, a linear or branched alcohol having 1 to 5 carbon atoms including butanol, ethyl acetate, methylene, chloride, chloroform, hexane, or a mixture thereof.

The extract of the present invention may be prepared according to a conventional method for producing a plant extract. More specifically, the preparation of the extract of Godeulbaegi was performed by adding an extraction solvent, preferably water, to the pulverized product obtained by pulverizing the dried product of Godeulpagi, preferably the freeze-dried product of Godeulbagi, from which impurities were removed. The extraction method using the solvent may be a hot water extraction method, a cold extraction method, a hot extraction method, a pressure extraction method, a reflux extraction method or an ultrasonic pulverization extraction method, preferably a hot water extraction method.

In addition, the preparation of the fraction of the extract of Godeulbaegi can be performed by adding a solvent to the extract prepared by the above extraction method, that is, the crude extract or the crude extract, and then obtaining the fraction according to the polarity of the fractionation solvent. The method for obtaining the fraction may be performed by a separation method by layer separation or a fractionation method. More specifically, after adding a fractionation solvent having a different polarity from the extraction solvent to the extract, for example, hexane, chloroform, methylene chloride, ethyl acetate, butanol and water fractionation solvents are added in the same order as described above, followed by layer-separated hexane fraction , a chloroform fraction, a methylene chloride fraction, an ethyl acetate fraction, a butanol fraction and a water fraction.

The fractionation method by layer separation may be a method of adding to the extract in that order according to the degree of nonpolarity of the solvent, and obtaining a fraction obtained by layer separation for each application, for example, by adding hexane to the ethanol aqueous solution extract a hexane fraction obtained by fractionating the layer-separated hexane layer; a methylene chloride fraction obtained by fractionating the methylene chloride layer separated by the addition of methylene chloride to the layer remaining after separating the hexane fraction; The ethyl acetate fraction obtained by fractionating the layer-separated ethyl acetate layer by adding ethylene acetate to the layer remaining after separating the methylene chloride fraction and the water fraction remaining after separating the ethyl acetate fraction may be sequentially obtained.

Chromatography for purification of the fraction is silica gel column chromatography (silica gel column chromatography), thin layer chromatography (TLC), high performance liquid chromatography (high performance liquid chromatography, HPLC), such as various chromatography using can be done by

In addition, the extract may be concentrated or the solvent may be removed by performing extraction, fractionation, or purification, followed by filtration under reduced pressure or additional concentration and/or freeze-drying. Therefore, the extract of Godeulbaegi in the present invention is used in the sense of including a dried product of the extract dried by a conventional method, a concentrate of the extract concentrated by a conventional method, and a diluted solution of the extract, dried product or concentrate. The obtained Godeulbaegi extract may be stored in a deep freezer until use.

Specifically, the method for obtaining the extract of Godeulbaegi includes a step of preparing a sample of Godulpagi extract, which is washed in running water, dried at -20°C, and then ground to prepare a milled product; And it may be prepared by a method comprising the step of adding water to the extract sample and extracting the extract with hot water at 80°C to 120°C.

In this aspect, the Godeul-baegi extract, which is an active ingredient of the whitening composition, is dried in running water at -20°C, and then grinded to prepare a Godeul-baegi extract sample preparation step; And it may be prepared by a method comprising the step of adding water to the extract sample and extracting the extract with hot water at 80°C to 120°C.

The melanin is a black-brown pigment and is present in the skin, hair, or eyes. In a way that absorbs more than a certain amount of ultraviolet rays, it blocks the penetration of ultraviolet rays to protect the human body, maintain body temperature, and skin color is determined by the amount of melanin.

When the melanin is produced abnormally low, skin lesions such as vitiligo are induced, while, on the other hand, when it is overproduced by external environmental factors, etc., it causes pigmentation such as spots and freckles and skin blemishes, resulting in cosmetic problems. In addition, it is closely related to skin cancer and can be a cause of disease.

Therefore, the whitening composition of the present invention capable of decomposing the melanin can be applied as a skin external pharmaceutical composition for whitening or a cosmetic composition for whitening.

The Godeulpagi extract prepared by the production method of the present invention may be preferably a hot water extract of Godeulpagi, more preferably a hot water extract of a freeze-dried product of Godeulpagi. The hot water extract of the lyophilized product of Godeulpaegi has excellent tyrosinase inhibitory activity related to melanin production, and above all, because it uses Godeulbaegi and water, which have been used for food for a long time, safety such as side effects or toxicity is not a problem, and the extraction yield Also, the whitening composition has excellent marketability in various aspects.

In this aspect, the present invention may be a pharmaceutical composition for external use for skin whitening comprising the extract of Godeulpaegi as an active ingredient. The extract of Godeulbaegi may be preferably a hot water extract.

The skin external pharmaceutical composition for whitening may further include appropriate carriers, excipients and diluents commonly used in the preparation of pharmaceutical compositions.

The skin external pharmaceutical composition for whitening may be formulated according to a conventional method, respectively, and is preferably formulated as a cream, gel, patch, spray, ointment, warning agent, lotion, liniment agent, pasta agent or cataplasma agent. may be used, but is not limited thereto.

Carriers, excipients and diluents that may be included in the external skin pharmaceutical composition for whitening include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

When the pharmaceutical composition for external use for skin whitening is formulated, commonly used diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants or surfactants may be used.

The formulation for parenteral administration of the pharmaceutical composition for external use for skin whitening may include a sterile aqueous solution, a non-aqueous solution, a suspension, an emulsion, a freeze-dried formulation, and a suppository.

As the non-aqueous solvent or suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used. In addition, as the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, or glycerogelatin may be used.

The preferred dosage of the pharmaceutical composition for external use for skin whitening varies depending on the patient's condition and weight, the degree of disease, drug form, administration route and period, but may be appropriately selected by those skilled in the art. As an example, the composition of the present invention may be administered at 0.000001 to 100 mg/kg (active ingredient/body weight) per day based on the active ingredient, preferably 0.001 to 10 mg/kg (active ingredient/body weight). However, the above dosage does not limit the scope of the present invention in any way. The external administration of the pharmaceutical composition for external use for skin whitening may be administered once a day or divided into several administrations.

In addition, in another aspect, the present invention may be a cosmetic composition for whitening comprising the extract of Godeulpaegi as an active ingredient. The Godeulpagi extract is preferably a Godeulpagi hot water extract, more preferably a Godeulpagi extract sample preparation step of washing the Godeulpagi washed in running water at -20°C, and then grinding to prepare a milled product; And it may be prepared by a method comprising the step of adding water to the extract sample and extracting the extract with hot water at 80°C to 120°C.

The active ingredient of the cosmetic composition for whitening, the extract of Godeulpagi, has excellent tyrosinase inhibitory activity in relation to melanin production and has been recognized for safety, so it can be used in various ways, such as cosmetics and face washes having a whitening effect. Products to which the active ingredient can be added include, for example, cosmetics such as various creams, lotions, and skins, and cleansing agents, face washes, soaps, treatments, and cosmetics.

The cosmetic composition for whitening may further include at least one selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract.

The water-soluble vitamins can maintain the effects of the present invention, and can be included in any form as long as they can be formulated in cosmetics. Specifically, vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide , folic acid or vitamin C, salts thereof (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives thereof (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate, etc.) . The water-soluble vitamin may be obtained by a conventional method such as a microorganism transformation method, a purification method from a microbial culture, an enzymatic method or a chemical synthesis method.

The oil-soluble vitamins can maintain the effects of the present invention and may include any type of compound that can be formulated in cosmetics. Specifically, vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (D-alpha tocopherol, D-alpha tocopherol) , D-alpha tocopherol), and their derivatives (ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, acetate-alpha tocopherol, nicotinic acid-alpha tocopherol vitamin E, D-pantothenyl alcohol, D- pantothenyl alcohol, pantothenyl ethyl ether, etc.). The oil-soluble vitamin can be obtained by a conventional method such as a microorganism transformation method, a purification method from a microbial culture, an enzymatic method or a chemical synthesis method.

The polymer peptide can maintain the effects of the present invention, and may be included in any form as long as it can be formulated in cosmetics, and specifically, it may be collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin or keratin. The polymer peptide can be obtained through a purification process after being obtained by a conventional method such as a purification method from a culture solution of a microorganism, an enzyme method, or a chemical synthesis method. It can also be purified from and used.

The high molecular weight polysaccharide can maintain the effects of the present invention and may include any compound that can be formulated in cosmetics, specifically hydroxyethyl cellulose, xanthan gum, sodium hyaluronate or chondroitin sulfate, and their salts (sodium salt, etc.). The chondroitin sulfate or a salt thereof may be purified and used from mammals or fish.

The sphingolipid may maintain the effects of the present invention and may include any compound that can be formulated in cosmetics, and specifically may be ceramide, phytosphingosine, or sphingoglycolipid. The sphingo lipid may be purified by a conventional method from mammals, fish, shellfish, yeast or plants, or may be obtained by chemical synthesis.

The seaweed extract can maintain the effect of the present invention, and can include any that can be formulated in cosmetics, specifically brown algae extract, red algae extract, green algae extract, etc., and the seaweed extract includes a color purified from the seaweed extract. Also included are ginan, arginic acid, sodium alginate, potassium alginate, and the like. The seaweed extract may be obtained by purification from seaweed by a conventional method.

In addition, the cosmetic composition of the present invention may further include other ingredients commonly formulated in cosmetics. More specifically, the other ingredients commonly formulated in the cosmetic are oil and fat components, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters , alcohol, color, fragrance, blood circulation promoter, cooling agent, limiting agent, or purified water.

Specifically, the fat and oil component may be an ester-based fat, hydrocarbon-based fat, silicone-based fat, fluorine-based fat, animal fat, or vegetable fat.

The ester-based oils and fats are, for example, tri-2-ethylhexanoate glyceryl, 2-ethylhexanoate cetyl, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearic acid Socetyl, butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, sebacic acid Diethyl, diisopropyl adipate, isoalkyl neopentanoate, tri(capryl, capric acid) glyceryl, tri-2-ethylhexanoate trimethylolpropane, triisostearate trimethylolpropane, tetra-2- Pentalyslitol ethyl hexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, ricinoleic acid Cetyl, isostearyl laurate, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isostearate isopropyl, cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, di(capryl, capric acid) propylene glycol, Propylene glycol dicaprylate, neopentyl glycol dicapric acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecyl acid, glyceryl triisopalmitate, glyceryl triisostearate, octyldode neopentanoate Thread, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearisostearate, octyldecyl isostearate, polyglycerol oleic acid ester, Polyglycerol isostearate, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate , trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 1,2-stealoylhydroxystearate isocetyl, 1,2-stealoylhydroxystearate stearyl, or 1,2-stealoylhydroxystearate isostearyl.

The hydrocarbon-based oil may be, for example, squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax or petrolatum.

The silicone-based fat is, for example, polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxanemethylcetyloxysiloxane copolymer, dimethylsiloxane methylstealloxysiloxane copolymer, It may be an alkyl-modified silicone oil or an amino-modified silicone oil.

The fluorine-based oil may be perfluoropolyether or the like.

The animal oil or vegetable oil is avocado oil, almond oil, olive oil, sesame oil, rice bran oil, bird flower oil, soybean oil, corn oil, rapeseed oil, sesame oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, Cottonseed oil, palm oil, kukui nut oil, wheat germ oil, rice germ oil, shea butter, cranberry colostrum, marker damia nut oil, meadow home oil, egg yolk oil, tallow, horse oil, mink oil, orange rafi oil, jojoba oil, candelabra wax , carnauba wax, liquid lanolin, or hydrogenated castor oil.

The surfactant may be specifically an anionic surfactant, a cationic surfactant, or an amphoteric surfactant.

The anionic surfactant is, for example, fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng Salt, alkylamide phosphate, alkyloyl alkyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, alkyl sulfoacetate sodium, acylated hydrolyzed collagen peptide salt or perfluoroalkyl phosphate ester etc.

The cationic surfactant is, for example, alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyl bromide trimethylammonium, benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, or a quaternary ammonium salt of a lanolin derivative.

The amphoteric surfactant is, for example, a carboxybetaine type, an amidebetaine type, a sulfobetaine type, a hydroxysulfobetaine type, an amidesulfobetaine type, a phosphobetaine type, an aminocarboxylate type, an imidazoline derivative type or an amide It may be an amine-type amphoteric surfactant or the like.

The pigment may be an organic pigment, an inorganic pigment, and a complex pigment thereof.

The inorganic pigment is, for example, silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, oxide aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, and complexes thereof.

The organic pigment is, for example, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinyl It may be a benzene-styrene copolymer, silk powder, cellulose, CI pigment yellow, CI pigment orange, and complexes thereof.

The organic powder may include, for example, metal soap such as calcium stearate; Alkyl phosphate metal salts, such as zinc sodium cetylate, zinc laurylate, and calcium laurylate; acylamino acid polyvalent metal salts such as calcium N-lauroyl-beta-alanine, zinc N-lauroyl-beta-alanine, and calcium N-lauroyl glycine; amidesulfonic acid polyvalent metal salts such as calcium N-lauroyl-taurine and calcium N-palmitoyl-taurine; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hydrogenated beef fatty acid acylarginine, etc. -acyl basic amino acids; N-acyl polypeptides such as N-lauroylglycylglycine; alpha-amino fatty acids such as alpha-aminocaprylic acid and alphaaminolauric acid; It may be polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, ethylene tetrafluoride, or the like.

The cosmetic composition for whitening may be prepared in any formulation conventionally prepared in the art, for example, it may be prepared as an emulsion, cream, lotion, pack, foundation, lotion, cosmetic liquid, hair cosmetic, and the like.

Specifically, the cosmetic composition for whitening is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, It may be formulated in any one formulation selected from the group consisting of a pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser, but is not limited thereto.

When the formulation of the cosmetic composition for whitening of the present invention is a paste, cream, or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or oxide as a carrier component Zinc or the like may be used.

When the formulation of the cosmetic composition for whitening of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. propellants such as hydrohydrocarbons, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition for whitening of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent may be used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl Benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.

When the formulation of the cosmetic composition for whitening of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester are suspended such as agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like can be used.

When the formulation of the cosmetic composition for whitening of the present invention is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarco Cinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester and the like can be used.

In addition, the present invention relates to a method for preparing an extract of Godeulbaegi having excellent whitening effect.

The method for preparing an extract of Godeulbaegi having excellent whitening effect includes: preparing a sample of Godeulbaegi extract, which is washed in running water, dried at -20°C, and then ground to prepare a milled product of Godeulbaegi; and adding water to the sample of the extract of Godeulbaegi and extracting the extract with hot water at 80°C to 120°C.

As described above, the extract of Godeulbaegi, an active ingredient of the present invention, not only has excellent tyrosinase inhibitory activity in relation to the whitening effect, but also uses Godeulbaegi, which has been used for food for a long time, and water, which is the safest solvent, so there are problems such as side effects. is not generated, safety is recognized, and has the advantage of being environmentally friendly, so the composition of the present invention may be applied to various uses related to melanin, including skin whitening.

Figure 1 shows the antioxidant effect according to the extraction solvent and drying method of Godeulpagi extract according to an embodiment of the present invention. It is a graph showing the experimental results on the effect, that is, ABTS radical scavenging activity, and FIG. 1b is the antioxidant effect of the extract, that is, ABTS radical scavenging activity (ABTS) It is a graph showing the experimental results for radical scavenging activity).
2 is a graph showing the antioxidant effect according to the extraction solvent and drying method of the extract according to an embodiment of the present invention, the horizontal axis is the result according to the drying method and the extraction solvent, and the vertical axis is an experiment on tyrosinase activity This is a graph showing the results.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.

All experimental results were repeated at least 3 times and expressed as the average value, and the comparison with each group was judged to be significant when p <0.05, respectively, using the ANOVA test.

[ Example . Confirmation of the optimal whitening effect of the extract

Example 1. Preparation of extract

The extracts from Godeulbaragi used in this experiment were prepared in the following way.

For the sample of Godeulbaegi, after collecting the godeulbaegi grown at a nearby farmhouse in Yeonggwang, Jeollanam-do, washed in running water, freeze-dried using a freezer at - 20℃ or hot-air dried using a hot air dryer at 60℃, and then pulverized using a grinder. was prepared. It was prepared by adding an extraction solvent, specifically purified water, 50% aqueous ethanol solution, or 100% ethanol aqueous solution, to the sample, which is a pulverized product prepared by each of the above methods, and extracting it. In the case of the purified water, 10 g of the Godeulbaegi sample was added to 200 mL of water and hot water extraction was performed at 110°C for 6 hours. It was carried out by adding to 200 mL of an aqueous solution and extracting at 60° C. for 24 hours.

The solvent extracts prepared by each of the above methods were filtered using filter paper (NO.2 Whatman), and then the resulting filtrate was concentrated by rotary vacuum concentration to prepare a concentrate, and the concentrate was freeze-dried. was prepared by the method Each extract finally obtained by lyophilization was stored at -20°C and used in the experiment.

Table 1 below shows the extraction yield (FD yield) by the method for preparing the Godeulbaeggi sample (dry conditions) and the extraction solvent.

drying conditions solvent type Yield (g) transference number(%) hot air dry
(60℃) water solvent 2.90 29.0 50% ethanol aqueous solution 2.75 27.5 100% ethanol 0.73 7.3 freeze drying
(-20℃) water solvent 4.02 40.2 50% ethanol aqueous solution 3.92 39.8 100% ethanol 0.75 7.5

As shown in Table 1, it was confirmed that the extraction yield was higher in the freeze drying method than in the hot air drying method as a whole, and in the case of the extraction solvent, the yield was the highest in the hot water extraction method using an aqueous solvent, and in the case of the extraction method using 100% ethanol It was confirmed that the extraction yield was significantly reduced.

To confirm the safety of each extract, a cytotoxicity experiment using Melanoma (B16F10) cells was performed. Specifically, Melanoma (B16F10) cells were aliquoted to 5 x 10 ⁴ cells/well in a 96 well plate and cultured for 24 hours. Incubated in ₂ incubator for 5 days. After the incubation, 0.02 mL of MTT solution prepared at a concentration of 5 mg/mL was added and incubated for 4 hours, the culture solution was removed, and 0.2 mL of dimethyl sulfoxide (DMSO) was added to each well to dissolve the formazan crystal product, By measuring the absorbance at a wavelength of 570 nm with an ELISA reader, the cell growth rate was measured.

As a result of measuring the cell growth rate, the extraction sample was compared with the control (con) in which no sample was added at 25 μg/mL, 50 μg/mL, and 100 μg/mL, regardless of all kinds of drying methods and extraction solvents. It was confirmed that there was no cytotoxicity because the cell growth rate was similar.

Example 2. Measurement of antioxidant activity according to the type of extract

The antioxidant activity of the extract obtained in Example 1 was measured using ABTS free radical scavenging activity. Specifically, the ABTS free radical scavenging activity was measured by mixing ABTS with a concentration of 2 mM and potassium persulfate with a concentration of 3.5 mM, dissolved in 10 ml of tertiary distilled water, and then diluted 30 times by adding 290 ml of tertiary distilled water, Overnight reaction was induced to generate ABTS radicals. 10 µL of Godeul-baegi extract prepared in Example 1 was added to sodium phosphate buffer (0.1M, pH6.8) at respective concentrations, and 290 µL of ABTS radical solution were added and reacted for 10 minutes at room temperature, Absorbance was measured at 750 nm. In order to contrast with the ABTS radical inhibition rate of the extract, the results of comparison with the scavenging ability of the control (con) to which no sample was added are shown in FIG. 1 .

As shown in FIG. 1a, when dried godeulbaegi by hot air drying method, when 50% ethanol aqueous solution was used, the antioxidant activity was confirmed to be the best, and in the case of 100% ethanol, antioxidant activity was hardly confirmed, whereas In the case of drying by freeze-drying, it was confirmed that the antioxidant activity was decreased in the case of hot water extraction and 50% ethanol aqueous solution, whereas the antioxidant activity was improved in the case of 100% ethanol.

Example 3. According to the type of extract whitening activity Measure

The whitening activity by the extract obtained in Example 1 was measured.

Specifically, the whitening activity of the extract obtained in Example 1 was confirmed through inhibitory activity against tyrosinase related to melanin production. Specifically, the B16F10 cells were treated with α-melanocyte stimulating hormone (α-MSH) and the extract prepared in Example 1 and cultured for 72 hours. After incubation, wash with phosphate buffer (pH 7.4) and centrifuge to obtain a cell precipitate, add 100 μL of 10 mM phosphate buffer (pH 6.8) containing 1% (w/v) triton X-100, and shake for 5 minutes After that, both the cells and the solution were transferred to an eppendorf tube, centrifuged, and the supernatant was used to measure tyrosinase activity. 40 μL of the supernatant obtained after sample treatment was dispensed in a 96-well plate, and 40 μL of 10 mM L-DOPA dissolved in 0.1M sodium phosphate buffer (pH 7.2) was added and incubated at 37° C. for 30 minutes. The absorbance of DOPA chrome produced by tyrosinase was measured at 475 nm. The measurement results are shown in FIG. 2 by confirming the inhibitory activity of each extract compared to the tyrosinase inhibitory activity of the control (con) treated with arbutin, which is a positive control.

As shown in FIG. 2, in the case of ethanol or 50% ethanol aqueous solution, it was confirmed that the tyrosinase inhibitory activity was higher when extracted with respect to the hot air-dried extract, whereas in the hot water extraction method, the extract for the lyophilized product of Godeul-baegi was Thai. It was confirmed that the rosinase inhibitory activity was higher.

Therefore, in terms of whitening activity related to tyrosinase inhibitory activity and in terms of extraction yield, it was confirmed that the extract extracted with hot water after adding water to dry matter dried at −20° C. was confirmed to be the best.

Although preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (7)

The extract ( Youngia ) sonchifolia ) as an active ingredient. According to claim 1,
The whitening composition, characterized in that the extract is a hot water extract. The extract ( Youngia ) sonchifolia ) as an active ingredient, a pharmaceutical composition for external use for skin whitening. The extract ( Youngia ) sonchifolia ) as an active ingredient, a cosmetic composition for whitening. 5. The method of claim 4,
The cosmetic composition for whitening, characterized in that the extract is a hot water extract. 5. The method of claim 4,
The Godeulbaegi extract is dried in running water at -20 ℃, and then ground to prepare a Godeulbagi extract sample preparation step; And Whitening cosmetic composition, characterized in that it is prepared by a method comprising the step of adding water to the extract sample of Godeulbaegi and extracting it with hot water at 80°C to 120°C. After drying the godeulbaegi washed in running water at -20°C, grinding the godeulbaegi extract sample preparation step to prepare a milled plant; And adding water to the extract sample of Godeulbaegi and extracting the extract with hot water at 80°C to 120°C
A method for producing an extract of Godeul-baegi with excellent whitening effect, comprising:

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