KR20190003226A - A whitening composition comprising youngia sonchifolia extract - Google Patents

Abstract

The present invention relates to a whitening composition comprising a youngia sonchifolia extract as an active ingredient and a method of preparing the youngia sonchifolia extract which has a superior whitening effect. The present invention especially has the superior whitening effect, has a good antioxidant effect, uses the youngia sonchifolia which was used as a food and a medicine for a long time, so that a problem related to tolerance and stability does not occur. Therefore, the whitening composition can be used in various ways including whitening cosmetics.

Description

A WHITENING COMPOSITION COMPRISING YOUNGIA SONCHIFOLIO EXTRACT < RTI ID = 0.0 >

The present invention relates to a whitening composition comprising a plant extract, specifically a rosemary extract, as an active ingredient.

The skin color of a person is determined by the concentration and distribution of melanin, keratin and hemoglobin in the skin. In addition to the genetic factors, the skin color is also affected by environmental conditions such as ultraviolet rays, fatigue, stress, and other physiological conditions. Melanin is the most important factor affecting the skin color.

Melanin (melanin) is a representative coloring matter that plays an important role in protecting the skin and protecting the skin from damage caused by ultraviolet rays (UV). The melanin is produced by melanocyte cells in the basal layer of the skin epidermis. Melanin is produced by a nonenzymatic oxidation reaction after conversion of tyrosinase (EC: 1.14.18.1), which is a kind of amino acid, to dopa and dopaquinone. The tyrosinase is one of copper ion-containing monooxygenases containing a copper atom and is an important enzyme in the melanin biosynthesis pathway as described above .

The melanin synthesized by the melanocyte cells transitions to peripheral keratinocytes and shows the color of human skin.

If the melanin is excessively produced in the skin excessively, it darkens the skin color, causes spots, freckles or dots, and causes hyperpigmentation and the like, resulting in poor cosmetic results. Therefore, inhibiting the synthesis of melanin pigment in skin can brighten skin color to achieve whitening of skin, as well as improve skin hypercholesterolemia, such as spots and freckles caused by ultraviolet rays, hormones, and genetic causes.

In recent years, as interest in beauty and desire for self-management has increased and interest in skin has increased, research on skin whitening has been actively carried out to remove dullness of skin such as dermis, and on the other side, The demand for preventing the deposition of melanin pigment due to ultraviolet rays is increasing due to the increase in the population enjoying leisure activities in the field.

Reduction of the biosynthesis of the melanin pigment can be accomplished by inhibiting tyrosinase, reducing exposure by ultraviolet (UV), or inhibiting melanocyte cells. Among the above methods, a component capable of inhibiting tyrosinase was used as an active ingredient in relation to existing whitening cosmetics.

With respect to the whitening cosmetic product, conventionally, a substance having inhibitory activity against tyrosinase such as hydroquinone, ascorbic acid, kojic acid and glutathione, which is a chemical ingredient, is added to a cosmetic such as an ointment for external use, a cream or an essence Thereby achieving skin whitening and improving skin hypercholesterolemia such as spots and freckles.

However, these ingredients are mainly synthetic substances, and safety problems are raised, and there are problems such as their efficacy and deterioration when they are used together with cosmetics.

Specifically, there is a problem that hydroquinone has a problem of limiting the amount of the skin to a very small amount due to severe skin irritation, and recently, problems such as induction of allergy or induction of skin cancer have been raised, Is classified as a medicament requiring a dermatologist's prescription, and it is regulated that a concentration of less than 1% of the total composition can be added to the external composition sold in hospitals or pharmacies.

In addition, ascorbic acid, which is a vitamin C derivative, is easily oxidized, and the cosmetic composition containing the vitamin C derivative causes problems such as discoloration and detachment, resulting in deterioration of the quality of the product, and the whitening effect is deteriorated accordingly. In recent years, derivatives such as Ascorbyl-palmitate, Ascorbyl-dipalmitate, Ascorbyl stearare, or Ascorbyl magnesium phosphate have been developed to solve this problem. However, it has been suggested that the difficulty of penetration into the skin is difficult unless a special technique is used.

In addition, kojic acid is likely to be carcinogenic when administered orally, and thus it is necessary to further verify safety. Further, thiol compounds such as glutathione and cysteine have not only unpleasant odors but also problems with transdermal absorption, and their glycosides and derivatives It has a disadvantage that it is difficult to use it as a blending component of cosmetics because of its high polarity.

In addition, substances having tyrosinase inhibitory activity isolated from existing natural materials mainly have a phenol structure or a metal chelating agent, and substances having inhibitory activity are low in activity However, its use is limited due to high toxicity and insufficient penetrative ability.

Accordingly, there is an increasing need for studies on ingredients that can dissolve melanin itself, for example, as an effective ingredient of whitening cosmetics, which is more effective than whitening agents so far and safer.

KR 10-2004-0018733A KR 10-0527602B

The present invention has been made to solve the problems of the prior art as described above. In particular, it is an object of the present invention to provide an effective ingredient and its optimum content of a skin whitening composition containing a natural substance effective for skin whitening, do.

In order to achieve the above object, the present invention provides a whitening composition comprising Youngda sonchifolia as an active ingredient.

The rodain extract may preferably be a hot-water extract, more preferably a hot-water extract of a roast lemon-freeze.

The whitening composition can be used as a pharmaceutical composition having a whitening effect or a cosmetic having a whitening effect, and in this respect, it can be a whitening dermatological pharmaceutical composition or a whitening cosmetic composition.

In this aspect, the invention godeulppaegi extract (Youngia sonchifolia ) as an active ingredient. The rodain extract may preferably be a hot-water extract, more preferably a hot-water extract of a roast lemon-freeze.

In another aspect, the present invention relates to a method of extracting rosemary extract sonchifolia ) as an active ingredient.

The rodent extract is prepared by drying a hot water extract, preferably a hot water extract of lyophilized lyophilized product, more preferably a lyophilized lyophilized lyophilized product, at -20 캜 and then grinding to prepare a lyophilized extract sample; And a step of preparing a rod extract by adding water to the sample of the rod extract and subjecting it to hot water extraction at 80 ° C to 120 ° C.

In another aspect, the present invention relates to a method for preparing a lobster extract sample preparation, which comprises drying a lobster washed in an oil water at -20 캜, followed by grinding to produce a lobster extract; And a step of preparing water extract of the rodula extract, wherein water is added to the sample and the water is extracted at 80 to 120 ° C.

Unless otherwise specified in the present specification, an effective component means a component that provides a whitening effect in a whitening composition, specifically, a pharmaceutical composition or a cosmetic composition.

As used herein, " prophylactic " means any action that inhibits the symptoms of a particular disease or delays its progress by administration of the composition of the present invention.

Unless otherwise specified herein, treatment refers to any remedial action that alters or alleviates the symptoms of a particular disease upon administration of the composition of the present invention.

Unless otherwise specified herein, an individual refers to any animal, such as a human, monkey, dog, goat, pig or rat, having a disease in which the symptoms of a particular disease, including gout, may be improved by administering the composition of the present invention .

Administration, as used herein, unless otherwise indicated, means providing the subject invention with a composition of the invention in any suitable manner.

As used herein, unless otherwise specified, pharmacologically acceptable means physiologically acceptable and does not cause an allergic reaction such as gastrointestinal disorder, dizziness, or the like, when administered to an animal, preferably a human, do.

The inventors of the present invention have been studying a substance having a whitening activity derived from a natural substance which does not have a problem with respect to stability such as side effects and tolerance. Among the various plants, the black tea extract having excellent whitening effect, specifically tyrosinase- It is confirmed that there is no problem in safety such as hydroquinone or kojic acid which is used in the whitening active composition of the composition or that there is no problem in formulation due to unpleasant odor such as thiol compound such as glutathione or cysteine, The present invention is further directed to a whitening composition comprising an effective ingredient and a method for producing a whitening composition, which comprises an extract of Rhodiola contained as an active ingredient in a whitening composition, wherein a hot water extraction method using only water as a solvent as a solvent is most effective for whitening, In preparing the powder , And that the lyophilization process is the best to perform lyophilization, and the present invention has been completed on the basis thereof.

Hereinafter, the present invention will be described in more detail.

The invention godeulppaegi (Youngia sonchifolia ) extract as an active ingredient. The rodain extract may be a hot-water extract. The whitening composition may be used for pharmaceutical or cosmetic purposes.

In this respect, the whitening composition may be a whitening dermatological pharmaceutical composition or a whitening cosmetic composition.

The rodain extract is prepared by drying a hot-water extract, specifically rodents washed in water, at -20 캜, and then grinding to prepare a rod-like extract sample; And a step of preparing a rhodochrosite extract by adding water to the sample of the rod extract and subjecting it to hot extraction at 80 to 120 캜, preferably at 110 캜.

The young animals sonchifolia ) is a perennial herbaceous plant belonging to the dicotyledonous plants of the dicotyledonous plant. It is also called a herb or herb. The stalks are straight and have many branches and have a purplish red color. Leaves are egg-shaped. Leaves around the roots have no hairs, and the length is 2.3 to 6 cm. The lower part is widened to cover the stem and has irregularly serrated teeth The flower blooms from May to July in yellow color. The flower of the head is hanging at the end of the branch. The corolla is yellow and the end is split. The fruit is black and the fruit is black. It ripens in the month.

The goddess exhaust is mainly distributed in the south central part of Korea, and is grown in the farms in the vicinity of the mountainous plants and fields. The young leaves and roots of the stomachs were immersed in kimchi and eaten as herbs.

The rodent extract may be a solvent extract extracted with an extraction solvent, a fraction obtained by adding a fraction solvent to an extract prepared by extracting with an extraction solvent, or a purified product obtained by performing chromatography on the fraction.

The extraction solvent may be any one selected from the group consisting of water, an organic solvent or a mixed solvent thereof, preferably water, an alcohol having 1 to 5 carbon atoms, and a mixture thereof, which can be used for extracting natural products. The organic solvent may be a neutral solvent such as a non-polar solvent of a nucleic acid or dichloromethane, a ketone of 3 to 5 carbon atoms, or a polar solvent such as a linear or branched alcohol having 1 to 5 carbon atoms such as methanol or ethanol, a polar solvent such as ethyl acetate or acetone, And may be water, preferably an alcohol or alcohol aqueous solution of 1 to 5 carbon atoms, more preferably water.

The fractionating solvent may be water, an alkane having 5 to 7 carbon atoms, a straight-chain or branched alcohol having 1 to 5 carbon atoms including butanol, ethyl acetate, methylene, chloride, chloroform, hexane or a mixture thereof.

The rodula extract of the present invention may be one prepared by a conventional method for producing a plant extract. More specifically, the preparation of the harvest extracts is carried out by adding an extracting solvent, preferably water, to the pulverized product obtained by pulverizing the dried product of the pulverized lobsters, preferably the pulverized lobsters, from which the impurities have been removed. The extraction method using the solvent may be a hot water extraction method, a cold extraction extraction method, a warm extraction extraction method, a pressure extraction extraction method, a reflux extraction method, or an ultrasonic pulverization extraction method, preferably a hot water extraction method.

In addition, the fraction of the rodent extract may be prepared by adding a fraction solvent to the extract prepared by the above extraction method, that is, crude extract or crude extract, and then obtaining a fraction according to the polarity of the fraction solvent. The method for obtaining the fractions can be carried out by separation or fractionation by layer separation. More specifically, after a fraction solvent having a polarity different from that of the extraction solvent is added to the extract, a fraction solvent such as hexane, chloroform, methylene chloride, ethyl acetate, butanol and water is added in the same manner as described above, , Chloroform fractions, methylene chloride fractions, ethyl acetate fractions, butanol fractions and water fractions.

The fractionation by layer separation may be a method of adding the extract to the extract according to the degree of non-polarity of the solvent and obtaining a fraction obtained by layer separation in each application. For example, hexane may be added to the aqueous ethanol solution A hexane fraction obtained by fractionating the layer separated hexane layer; A methylene chloride fraction obtained by fractionating the methylene chloride layer by separating the hexane fraction and adding methylene chloride to the remaining layer; Separating the methylene chloride fraction and adding ethyl acetate to the remaining layer, separating the ethyl acetate layer obtained by separating the layer and the ethylacetate fraction obtained by separating the ethyl acetate fraction, and sequentially obtaining the water fraction as the remaining layer.

Chromatography for purification of the fractions can be carried out using various chromatographies such as silica gel column chromatography, thin layer chromatography (TLC), high performance liquid chromatography (HPLC) .

Further, the extract may be subjected to extraction, fractionation or purification, followed by vacuum filtration or further concentration and / or lyophilization to concentrate or remove the solvent. Accordingly, the rodula extract in the present invention is used to mean a dried product of a conventional dried extract and a diluted solution of the concentrated extract, the extract, the dried product or the concentrated product concentrated by a conventional method. The obtained rodain extract can be stored in a deep freezer until use.

Specifically, the method for obtaining the rodent extract comprises: preparing a rod extract sample for drying a rodent washed in an oil water at -20 캜, followed by grinding to produce a rodent granule; And a step of preparing a rod extract by adding water to the sample of the rod extract and subjecting it to hot water extraction at 80 ° C to 120 ° C.

In this respect, the rodent extract, which is an active ingredient of the whitening composition, is dried at -20 占 폚, washed with water, and then subjected to a grinding to prepare a roasted extract sample; And a step of preparing a rod extract by adding water to the sample of the rod extract and subjecting it to hot water extraction at 80 ° C to 120 ° C.

The melanin is a dark brown pigment, which is present in skin, hair or eyes. The penetration of ultraviolet rays is blocked by a method of absorbing a certain amount of ultraviolet rays to protect the human body and maintain body temperature, and skin color is determined by the amount of melanin.

When melanin is produced abnormally less, skin lesion such as vitiligo is induced. On the other hand, when the melanin is overproduced due to external environmental factors, cosmetic problems may occur because it causes chromosomal accumulation such as spots and freckles, It is also closely related to skin cancer and can be a cause of disease.

Therefore, the whitening composition of the present invention capable of decomposing melanin can be applied as a whitening dermatological pharmaceutical composition or a whitening cosmetic composition.

The harvest extract prepared by the method of the present invention can be preferably a roasted hot-water extract, more preferably a hot-water extract of a roasted lyophilized product. The hot-water extract of the lyophilized lyophilized product is excellent in thyrosinase-inhibiting activity associated with melanin production. In addition, since the herbaceous food and water used for a long time are used, safety such as side effects and toxicity are not a problem, , The whitening composition has excellent commercial properties in various aspects.

In this respect, the present invention may be a whitening dermatological pharmaceutical composition comprising a rosemary extract as an active ingredient. The rodula extract may preferably be a hot-water extract.

The whitening dermatological pharmaceutical composition may further comprise suitable carriers, excipients and diluents conventionally used in the production of pharmaceutical compositions.

The whitening dermatological pharmaceutical composition may be formulated according to a conventional method. Preferably, the whitening dermatological pharmaceutical composition may be formulated into a cream, a gel, a patch, a spray, an ointment, an alarm, a lotion, a liniment, a pasta, But is not limited thereto.

Examples of the carrier, excipient and diluent which can be contained in the whitening dermatological pharmaceutical composition include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, and the like.

When the whitening dermatological pharmaceutical composition is formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents or surfactants which are commonly used may be used.

Formulations for parenteral administration of the whitening dermatological pharmaceutical composition may include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, and suppositories.

Examples of the non-aqueous solvent or suspension include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin or glycerogelatin.

The preferred dosage of the whitening dermatological pharmaceutical composition will vary depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. For example, the composition of the present invention can be administered in an amount of 0.000001 to 100 mg / kg (effective ingredient / body weight) per day, preferably 0.001 to 10 mg / kg (effective ingredient / body weight) per day based on the active ingredient. However, the dosages do not in any way limit the scope of the invention. External administration of the whitening dermatological pharmaceutical composition may be administered once a day or several times.

In another aspect, the present invention may be a cosmetic composition for whitening comprising a rodent extract as an active ingredient. The rodain extract is preferably prepared by drying a roasted hot-water extract, more preferably roots washed in water, at -20 캜, and then grinding to prepare a roasted extract sample; And a step of preparing a rod extract by adding water to the sample of the rod extract and subjecting it to hot water extraction at 80 ° C to 120 ° C.

The rosemary extract, which is an effective ingredient of the whitening cosmetic composition, is excellent in thyrosinase inhibitory activity in relation to melanin production and is recognized as safe, and can be used variously in cosmetics and cleanser having a whitening effect. Examples of the product to which the above-mentioned effective ingredient can be added include cosmetics such as various creams, lotions, skins, etc., and cleansing, cleansing agents, soaps, treatments, and serum.

The whitening cosmetic composition may further comprise at least one selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin may be any vitamin B 1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, , Folic acid or vitamin C, salts thereof (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives thereof (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

The above-mentioned useful vitamins may contain any of those which can maintain the effect of the present invention and can be incorporated in cosmetics. Specific examples thereof include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (D-alpha tocopherol, , D-alpha tocopherol), and the like, and derivatives thereof (palmitic acid ascorbin, stearic acid ascorbin, dipalmitic acid ascorbin, acetic acid-alpha tocopherol, nicotinic acid-alpha tocopherol vitamin E, D-pantothenyl alcohol, Pantothenyl alcohol, pantothenyl ethyl ether, etc.). The useful vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

The polymeric peptide may contain any substance as long as the effect of the present invention can be maintained and can be incorporated into cosmetics. Specifically, it may be collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin or keratin. The polymeric peptide can be obtained by a conventional method such as a purification method from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, and can be obtained through a purification process. Usually, the polymeric peptide can be obtained from natural materials such as dyes such as pigs and cows, May be used.

The polymer polysaccharide may be any of those which can maintain the effect of the present invention and can be compounded in cosmetics. Specifically, it may be hydroxyethyl cellulose, xanthan gum, sodium hyaluronate or chondroitin sulfate, and salts thereof Salt, etc.). The chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

The sphingogly lipid may be any one that can maintain the effect of the present invention and can be incorporated in cosmetics, and may specifically be ceramides, phytosphingosine and sphingoglycolipids. The sphingolipids can be purified from mammals, fish, shellfish, yeast or plants by conventional methods or by chemical synthesis.

The seaweed extract may be any of those which can maintain the effect of the present invention and can be compounded in cosmetics. Specifically, the seaweed extract may be a brown algae extract, a red algae extract, a green algae extract, etc. The seaweed extract may contain a colony purified from the seaweed extract Arginic acid, sodium alginate, potassium alginate, and the like. The seaweed extract can be obtained by purification from seaweed by a conventional method.

In addition, the cosmetic composition of the present invention may further contain other ingredients usually added to the cosmetic. More specifically, the other ingredients to be added to the conventional cosmetic composition are selected from the group consisting of a preservative component, a moisturizer, an emollient, a surfactant, an organic pigment and an inorganic pigment, an organic powder, an ultraviolet absorbent, an antiseptic, a bactericide, , Alcohols, coloring matters, fragrances, blood circulation promoters, coolants, restraining agents or purified water.

The above-mentioned sustaining component may specifically be ester-based, hydrocarbon-based, silicon-based, fluorine-based, animal or vegetable oil, and the like.

Examples of the ester-based oil include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearic acid But are not limited to, isopropyl myristate, butyl stearate, butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylolpropane, trimethylolpropane triisostearate, trimethylolpropane triisostearate, tri-2-ethylhexanoate trimethylolpropane, tri-2-ethylhexanoate trimethylolpropane, Ethylhexanoic acid pentaerythritol, ethylhexanoic acid pentaerythritol, caprylic acid cetyl, lauric acid decyl, hexyl lauric acid, myristic acid myristate, myristic myristate, myristic acid stearyl, stearyl stearate, decyl oleate, Octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, isopropyl stearate, Ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol , Propylene glycol dicaprylylate, dicapric acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated acid glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyl Dodecylsuccinic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, octadecanoic acid, Stearyl, octyldecyl isostearate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisolealkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate , Triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, sebacic acid di But are not limited to, isopropyl myristate, isopropyl myristate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, Stearyl, 1,2-stearoylhydroxystearic acid isosityl, 1,2-stearoylhydroxystearic acid stearate, Reel or 1,2-stacking egg yl-hydroxy stearic acid and the like cetearyl source.

The hydrocarbonaceous oil may be, for example, squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, flowing isoparaffin, polybutene, microcrystalline wax or vaseline.

Examples of the silicone-based oil include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethyl polysiloxane, decamethyl polysiloxane, dodecamethyl cyclosiloxane, dimethylsiloxane methyl cetyloxysiloxane copolymer, dimethylsiloxane methyl stearoxysiloxane copolymer, Alkyl-modified silicone oil or amino-modified silicone oil.

The fluorine-containing oil may be perfluoropolyether or the like.

The animal or vegetable oil may be selected from the group consisting of avocado oil, almond oil, olive oil, sesame oil, rice bran oil, fresh flower oil, soybean oil, corn oil, rapeseed oil, goat oil, palm kernel oil, palm oil, castor oil, Soybean oil, soybean oil, cottonseed oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, meadow home oil, egg yolk oil, woji, mayo, mink oil, , Carnauba wax, liquid lanolin or hardened castor oil, and the like.

The surfactant may specifically be an anionic surfactant, a cationic surfactant or a positive surfactant.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylallylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng A salt of alkylamide phosphate, an alkyloyltaurine salt, an N-acylamino acid salt, a POE alkyl ether carboxylate, an alkylsulfosuccinate salt, an alkylsulfoacetate salt, an acylated hydrolyzed collagen peptide salt or a perfluoroalkyl phosphate ester And so on.

The cationic surfactant includes, for example, alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenylbromide Trimethylammonium chloride, benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate or lanolin derivative quaternary ammonium salt, and the like.

Examples of the amphoteric surfactant include carboxybetaine, amidebetaine, sulfobetaine, hydroxysulfobetaine, amidosulfobetaine, phosphobetaine, aminocarboxylate, imidazoline derivative or amide Amine-type amphoteric surfactant, and the like.

The pigment may be an organic pigment, an inorganic pigment, a composite pigment thereof, or the like.

Examples of the inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth oxychloride, zirconium oxide, magnesium oxide, Aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, chromamine and combinations thereof.

Examples of the organic pigments include polyamides, polyesters, polypropylenes, polystyrenes, polyurethanes, vinyl resins, urea resins, phenol resins, fluororesins, silicon resins, acrylic resins, melamine resins, epoxy resins, polycarbonate resins, Benzene styrene copolymers, silk powders, cellulose, CI Pigment Yellow, CI Pigment Orange and combinations thereof.

Examples of the organic powder include metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, ethylene tetrafluoride, and the like.

The whitening cosmetic composition may be prepared in any form conventionally produced in the art, and may be prepared, for example, as an emulsion, a cream, a lotion, a pack, a foundation, a lotion, a serum or a hair cosmetic.

Specifically, the whitening cosmetic composition may be a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizing cream, a hand cream, a foundation, But the present invention is not limited thereto, and may be formulated into any one form of a pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

When the formulation of the whitening cosmetic composition of the present invention is a paste, cream or gel, the carrier component may be animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, Zinc and the like can be used.

When the formulation of the whitening cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, Propane / butane, or dimethyl ether. ≪ RTI ID = 0.0 >

When the formulation of the whitening cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl Benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan, and the like.

When the formulation of the whitening cosmetic composition of the present invention is a suspension, the carrier component may be a suspending agent such as water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the whitening cosmetic composition of the present invention is an interface-active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurate, Fatty acid amide ether sulfate, alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative, or ethoxylated glycerol fatty acid ester.

In addition, the present invention relates to a method for producing a harvest extract having excellent whitening effect.

The method for preparing a rodent extract having excellent whitening effect comprises: preparing a rod extract extract sample, which is dried at -20 占 폚 and then ground to remove the rodents washed in water; And a step of preparing a rod extract by adding water to the rod extract sample and subjecting it to hot water extraction at 80 to 120 ° C.

As described above, the rodent extract of the present invention as an active ingredient of the present invention is excellent in inhibitory activity against tyrosinase in relation to the whitening effect, and also uses water as the safest solvent and the rodent which has been used for a long time, , Safety is also recognized, and it is environmentally friendly. Therefore, the composition of the present invention can be applied to various uses related to melanin including skin whitening.

FIG. 1 is a graph showing the antioxidative effect according to the extraction solvent of the rodula extract and the drying method of the rodula according to an embodiment of the present invention. FIG. 1 a shows the antioxidative effect of the extract on the rodula powder prepared by drying the rodula FIG. 1B is a graph showing the results of experiments on the ABTS radical scavenging activity, that is, the ABTS radical scavenging activity, and ABTS radical scavenging activity of the extract of rosemary powder prepared by freeze drying of rosemary radical scavenging activity).
FIG. 2 shows the antioxidative effect according to the extraction solvent of the rodula extract and the drying method of the rodula according to an embodiment of the present invention, wherein the horizontal axis shows the results according to the drying method and the extraction solvent, and the vertical axis shows the results of the experiments on the tyrosinase activity Fig.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

All experimental results were expressed as mean value repeatedly at least three times, and comparison of each group was determined to be significant in the case where each p <0.05 using ANOVA test.

[ Example . Determination of optimal whitening effect of rosemary extract]

Example  1. Preparation of rosemary extract

The Rhodiola extract used in this experiment was prepared by the following method.

The rhizome samples were collected from the nearby farmhouses in Yeonggwang area, Jeollanam-do, and then washed in the water, lyophilized using a freezer at 20 ° C or hot air dried at 60 ° C using a hot air drier, . Specifically, purified water, a 50% aqueous ethanol solution, or a 100% aqueous ethanol solution was added to a sample, which was a pulverized pulverulent manufactured by each of the above methods, and extracted. In the case of the purified water, 10 g of the rodent sample was added to 200 ml of water and the resultant was subjected to hot water extraction at 110 ° C for 6 hours. When the ethanol or ethanol aqueous solution was added, 10 g of the rod sample was dissolved in ethanol or 50% Was added to 200 mL of the aqueous solution and extracted at 60 캜 for 24 hours.

The solvent extract prepared by each of the above methods was filtered through a filter paper (NO.2 Whatman), and the resulting filtrate was concentrated by rotary vacuum concentration to prepare a concentrate. The concentrate was freeze-dried Lt; / RTI &gt; Each of the extracts obtained by freeze-drying was stored at -20 ° C and used for the experiment.

Table 1 below shows the preparation method (drying conditions) and extraction yield (FD yield) by the extraction solvent.

Drying conditions Solvent type Yield (g) yield(%) hot air dry
(60 DEG C) Water solvent 2.90 29.0 50% aqueous ethanol solution 2.75 27.5 100% ethanol 0.73 7.3 Freeze-dried
(-20 ° C) Water solvent 4.02 40.2 50% aqueous ethanol solution 3.92 39.8 100% ethanol 0.75 7.5

As shown in Table 1, it was confirmed that the extraction yield was higher in the freeze drying method than in the hot air drying method as a whole, and the extraction yield was the highest in the case of the extraction solvent using hot water extraction method using water solvent and in the case of the extraction method using 100% ethanol It was confirmed that the extraction yield was remarkably low.

In order to confirm the safety of each of the extracts, a cytotoxicity experiment using Melanoma (B16F10) cells was performed. Specifically, Melanoma (B16F10) cells were plated at a density of 5 × 10 ⁴ cells / well in a 96-well plate, and cultured for 24 hours. Then, samples of each of the herbal extracts were prepared and treated at 37 ° C. and 5% CO ₂ incubator for 5 days. After the incubation, 0.02 mL of MTT solution prepared at a concentration of 5 mg / mL was added and incubated for 4 hours. The culture solution was removed and 0.2 mL of dimethyl sulfoxide (DMSO) was added to each well to dissolve the formazan crystal product. Absorbance was measured at 570 nm wavelength using an ELISA reader and the cell growth rate was measured.

As a result of the measurement of the cell growth rate, it was found that the extract samples were prepared at a concentration of 25 μg / mL, 50 μg / mL and 100 μg / mL in the control (con) It was confirmed that there was no cytotoxicity due to the similar cell growth rate.

Example  2. Determination of antioxidative activity according to the kind of black pepper extract

The antioxidative activity of the Rhodiola extract obtained in Example 1 was measured using ABTS free radical scavenging activity. Specifically, the ABTS free radical scavenging activity was measured by mixing 2 mM ABTS and 3.5 mM potassium persulfate, dissolving in 10 ml of tertiary distilled water, adding 290 ml of tertiary distilled water, diluting 30 times, Overnight reaction to induce ABTS radical formation. 10 μL of the Rhodiola extract and 290 μL of the ABTS radical solution prepared by adding the rodula extract prepared in Example 1 to sodium phosphate buffer (0.1M, pH 6.8) were reacted at room temperature for 10 minutes, Absorbance was measured at 750 nm. In order to compare the inhibition rate of ABTS radicals of the rodent extracts, the results are shown in FIG. 1 in comparison with the elimination ability of the control (con) to which no sample is added.

As shown in FIG. 1A, when the roots were dried by the hot air drying method, 50% ethanol aqueous solution showed the highest antioxidative activity, whereas 100% ethanol showed almost no antioxidative activity. In case of freeze drying, antioxidant activity was decreased in hot water extraction and 50% ethanol aqueous solution, whereas 100% ethanol was found to improve antioxidant activity.

Example  3. Depending on the type of rosemary extract Whitening  Measure

The whitening activity of the rodent extract obtained in Example 1 was measured.

Specifically, the whitening activity of the rodent extract obtained in Example 1 was confirmed by the inhibitory activity against tyrosinase associated with melanin production. Specifically, the B16F10 cells were treated with α-melanocyte stimulating hormone (α-MSH) and the rodent extract prepared in Example 1, and cultured for 72 hours. After culturing, the cells were washed with phosphate buffered saline (pH 7.4), centrifuged to obtain a cell suspension, 100 μL of 10 mM phosphate buffer (pH 6.8) containing 1% (w / v) triton X-100 was added and shaking After the cells and solution were transferred to an eppendorf tube, the supernatant was used for tyrosinase activity measurement. The supernatant obtained after the sample treatment was dispensed in a 96-well plate and 40 μL of 10 mM L-DOPA dissolved in 0.1 M sodium phosphate buffer (pH 7.2) was added and incubated at 37 ° C. for 30 minutes. The absorbance of DOPA chrome produced by tyrosinase was measured at 475 nm. The inhibitory activity of each extract against the tyrosinase inhibitory activity of the control group (con) treated with arbutin, which is a positive control group, is shown in FIG.

As shown in FIG. 2, in the case of ethanol or 50% ethanol aqueous solution, it was confirmed that the extract of the hot air dried extract had higher inhibitory activity of tyrosinase, whereas in the hot water extraction method, It was confirmed that the inhibitory activity of rosinase was higher.

Therefore, in terms of whitening activity and extraction yields related to tyrosinase inhibitory activity, it was confirmed that the extract obtained by adding water to the dried product of dried lobules at -20 ° C and extracting with hot water was the most excellent.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims (7)

Godeulppaegi extract (Youngia sonchifolia ) as an active ingredient. The method according to claim 1,
Wherein the rodula extract is a hot-water extract. Godeulppaegi extract (Youngia sonchifolia ) as an active ingredient. Godeulppaegi extract (Youngia sonchifolia ) as an active ingredient. 5. The method of claim 4,
Wherein the rodent extract is a hot-water extract. 5. The method of claim 4,
The rodain extract is dried at -20 캜 and washed with water, and then subjected to a grinding to prepare a lobster extract sample preparation step; And a step of preparing a rod extract by adding water to the sample of the rod extract and subjecting it to hot water extraction at 80 to 120 ° C. Drying the lobsters washed with distilled water at -20 DEG C and then crushing the lobsters to prepare a lobsters; And a step of preparing a rhodochrosite extract in which water is added to the sample of the rodula extract and subjected to hot water extraction at 80 to 120 ° C
Wherein the method comprises the steps of:

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