KR20120113459A - Compositions for skin whitening comprising essential oils from artemisia, lemon and fennel - Google Patents

Abstract

PURPOSE: A composition containing essential oil isolated from plants is provided to effectively suppress tyrosinase activation and melanogenesis. CONSTITUTION: A composition for skin whitening contains 0.01-50 wt% of plant essential oil isolated from Artemisia argyi Lev. et Vant., Citrus limonum, Foeniclum vulgare, or pine needles as an active ingredient. The composition is a cosmetic composition or pharmaceutical composition. The cosmetic composition is manufactured in the form of a skin softener, astringent, lotion, milk lotion, moisture lotion, massage cream, nutrition cream, hand cream, essence, pack, soap, cleansing foam, body lotion, or body cleanser. The pharmaceutical composition is manufactured in the form of a cream, gel, patch, spray, ointment, liniment, paster, or cataplasma.

Description

Composition for skin whitening comprising essential oils from artemisia, lemon and fennel}

The present invention relates to a composition containing the essential oil components extracted from the leaves, lemon, fennel and pine, more specifically, plant essential oils extracted from one or more plants selected from the group consisting of leaves, lemon, fennel and pine as an active ingredient It relates to a composition for skin whitening to contain.

Pigmentation on the skin, such as blemishes and freckles, is due to an increase in melanin in the epidermis, and melanin is also an important factor in determining skin color. Melanin is made from organelles called melanosomes in the melanocytes present in the basal epidermal layer. That is, mitosis of melanocytes occurs by ultraviolet rays, and then melanocytes are activated. In activated melanocytes, the synthesis of tyrosinase is accelerated and melanin production is promoted and transported out of the epidermis and released (VJ Hearing and M. Jimenez., Int. J. Biochem, 19 (20), p.1141, 1987 I. Koiso., Fragrance J. 6, p39, 1990).

Naturally produced melanin is a mixture of alkali poorly soluble black melanin (numelanin) and alkali soluble yellow or red melanin (feomelanin) (Y. Tomita., Fragrance J , 6, p20, 1990). In general, melanin polymers are often referred to as numelanin, Prota (G. Prota., J. Invest . Dermatol., 75, p122, 1980) and Pavel (S. Pavel et al., Cancer) . Detection and Prevention , 6, p311., 1983) and the like, in addition to the 5,6-dihydroxy indole which has been considered until now, numelanin is not only a 5,6-dihydroxy indole-2-carboic acid which is a separate melanin monomer. However, it was found that it is produced from various melanin intermediate metabolites. The melanin production process in melanosomes is that tyrosine is oxidized by the enzyme tyrosinase to be dopa → dopaquinone → dopachrome → 5,6-dihydroxyindole → indole-5,6-quinone, followed by indole-5,6 It is known to produce melanin by the polymerization of -quinone. Among them, tyrosine-dopaquinone is involved in the enzyme tyrosinase, and since then, it is known that the contribution of automatic oxidation is large. It is well known that pigmentation caused by sunbathing, or blackening, is a result of increased melanin production by ultraviolet rays in sunlight.

Looking at the mechanism of action of melanin inhibition from melanin production in melanocytes, which is clearly identified to date, melanin production is inhibited by melanin production by melanocytes, a mechanism that inhibits melanin production as a result of cytotoxicity to melanocytes. It is largely divided into two mechanisms of suppressive action. Recently published findings indicate that melanin is produced not only by tyrosinase but also by tyrosinase related protein 1 (TRP-1) and tyrosinase-retained protein 2 (TRP-2). These are also important enzymes involved in the biosynthesis of melanin, so developing a substance that can inhibit these enzymes may be a way to prevent skin pigmentation (Marmol V. at al., FEBS Letter , 327 , p. 307, 1993; Winder AJ at al., Pigment Cell Research , 7 , p. 305, 1994).

As tyrosinase activity inhibitors developed to date, chelate-forming substances for copper ions of tyrosinase active sites, reducing agents such as vitamin C or kojic acid for reducing quinones to phenols, and bisulfite preparations for modifying tyrosinase itself are known. . In addition, hydroquinone, retinol or hydrocortisol are known as substances that inhibit the tyrosinase activity and exhibit a whitening effect.

The leaf of the aster is the Yellow Sea Artemisia argyi Lev . meat Vant .), A. princeps Pamp . there is . orientlis Hara ) or dried leaves of young A. montana Pamp . In Japan, A. montana Pamp and A. princeps Pamp .) And in China the yellow sea wormwood ( A. argyi Lev . et Vant .) and geographically distributed throughout Asia such as Korea, China, Japan, Mongolia and Taiwan. A stem that grows laterally in the ground and strikes branches again to sprout and grow in various places. The leaves sprouting from the roots are deeply divided into circular wing feathers, and the split leaves are 5-6 pieces of cut-outs or irregular sawtooth. The leaves attached to the stem are a little long oval, with no incision and simpler as they go up. Stems and leaves have a lot of hairs, and the underside of leaves has dense white hairs. Around September to October, small flowers of tubular flowers bloom down to the end of stems or branches.

Pharmacological activity in Chinese medicine is good for vomiting blood and dysentery, and for diarrhea mixed with nosebleeds, bleeding, and bloody pus, especially for treating swelling of the genital area and for treating women's irregular uterine bleeding. Hemorrhoids and wounds caused by trauma, stop abdominal pain, stabilize pregnancy, skin diseases such as scabies, chronic hepatitis, chronic gastritis, liver cirrhosis, pulmonary tuberculosis is also good. It can treat abdominal pain caused by poison, treat muscle cramps with crayfish and ovulation, and treat dysphagia after dysentery. Treat your stomach, soothe your symptoms, and treat your cold symptoms as if your back is cold.

The leaflets are 2.0 g of ash per 100 g, protein 7.7 g, iron 10.9 mg, fiber 3.7 mg, vitamin B2 0.23 mg, phosphorus 70 mg, sugar 4.0 g. It contains chemicals such as vitamin B 0.12 mg, calcium 140 mg, vitamin C 22 mg, vitamin A 7940 IU, lipid 0.8 g, and niacin 1.5 mg.

These leaves are attached to the affected area by picking leaves and drying them in insect bites or nosebleeds, bleeding and bruises from wounds, and attaching them to the affected area. It has been widely used because it is effective in warming the blood and activating the digestive organs. Dried leaflets are used to rub and soften them, and then to moisten them. In the old days, they were used as flint wicks and set on fire. Even in rural areas, they are used as mosquitoes to chase mosquitoes.

Lemon ( Citrus limonum ) is distributed in cool, climate-free places around the world, but is grown mainly in countries such as the United States and Italy. Its height is 4 ~ 5m, the leaves are 10cm long, the butterflies are 5cm, and there are no wing leaves. The leaves are light green and the back is reddish purple. Flowers are endemic or gunshot in the tip of branch or leaf axilla, with many variations, long and large petals, purple on the outside, white in the middle, and flower thread is separated into many, and anther is long. The flowers are pale purple all year round, especially in May-October, and egg-shaped fruits are harvested in the following spring from October. The fruit is strong in scent, the skin is pale yellow and soft, and the fruit juice is rich, sour, and contains a lot of vitamin C. Both fragrance and taste are refreshing and strong, and fruit can be preserved long after harvest.

Pectin, a by-product of lemon, is used for the treatment of intestinal diseases such as anti-bleeding agents and plasma extenders. The vitamin p contained in lemon serves as a supplement to C. It is one of the vitamins that must be taken by patients suffering from high blood pressure, arteriosclerosis, and cerebral hemorrhage. In addition, lemon promotes the secretion of pancreatic fluid from the pancreas of diabetic patients to lower sugar content and calories.

Lemon contains 60 mg of calcium, 13 mg of phosphorus, 0.2 mg of iron, 4 mg of sodium, 120 mg of potassium, vitamins of B₁ 0.06 mg, B₂ 0.03 mg, nicotinic acid 0.2 mg, C 90 mg, limonene, citronellal, pinene, linalool, phellandrene and The same chemical ingredient is included.

In the private sector, citric acid, calcium citrate, and lemon oil are important by-products of lemon. Citric acid is used to make drinks. Lemon oil is used for perfumes, medicines, cosmetics, soaps, and seasonings. Lemon juice is a very good natural cleanser. Drinking green juice of peeled skin in warm water can help cleanse the liver, gallbladder and kidneys, and help dissolve calcium accumulated in the kidneys or gallbladder. Export stools Lemon juice has a diuretic, sweating and antioxidant effect, which lowers blood cholesterol, refreshes, and swells or stings.

Fennel (Foeniclum vulgare ) is distributed throughout the world, especially in Chile and the Mediterranean. The plant height is 1.5-2m, the stem grows straight, the inside is empty, the leaves are long sheath-shaped, pointed at the end, and 3-4 branched like a feather. In early summer, small yellow flowers with five petals at the end of the branches bloom in an umbrella-shaped inflorescence. The flowers have yellow pollen bags, and the asteris is slightly inward and grows long, and the pistil is very small and grows in two. Fruits are split and sweet, with a sweet taste and aroma. Dried seeds are usually eaten and are 4-10cm long. It has low endurance in cold climates and can withstand up to about -5 ° C, but cannot withstand snow.

Fennel is an anti-inflammatory agent for antitussives in pediatrics. It is effective against gastrointestinal weakness, stomach cramps, chronic laxative constipation, intestinal cramps, antiseptic expectorants, bronchial catarrh, whooping cough, and relieve it. Drinking tea reduces menopausal symptoms, increases mother's milk, increases appetite and digests, and helps relieve stress and sleep well. Diuretic effect is also effective in weight loss and obesity prevention.

Fennel is trans-anethol (anisecamphor). chemicals such as fenchone, α-phellandrene estragol, comphene, α-pinene, anisaldehyde, anisic acid, pcymene, cis-anethol, oleic acid, quercetin-3-arabinoside, vitamin-C, dihydro-ascorbic acid, vitamin-B2 It is included.

In the private sector, fennel is added to the boiled water to make syrup and used as a food for babies, especially for children with toothache and toothache. Eating fennel tea or seeds can promote metabolism and relieve indigestion. Fennel tea is boiled with a spoonful of fennel seeds, which serves to release gas from the stomach, and the juice made from syrup is effective for chronic cough. Also used as seasoning. One of the foods that fleas dislike is spraying powder on pulp or vegetables to fight fleas.

Turpentine (Turpentine) is a refined oil (精油) as a colorless obtained from pine trees, to obtain by distillation of the raw rosin (生松津) a distillation or pine of the steam directly into the steam, else by distillation songgeunyu (松根油) oil ( There is a method of purifying and distilling again.

It is a colorless oil with a unique odor and most of it leaks at a specific gravity of 0.860? 0.875 and 150? 180 ° C. It is insoluble in water but soluble in ether, chloroform and carbon disulfide. Saengjinjin, which is damaged by crushing pine trunks, contains about 20% of terebin oil, which is yellow in nature, sticky, has a unique aroma and taste, and is usually hard when exposed to air. Evaporation results in volatile turpentine oil and a solid resin.

Terebin oil stimulates the central nerve to excite or soothe, and acts as a skin irritant, anti-inflammatory, disinfectant, blood pressure relieving agent, expectorant, antihistamine, tonic and fatigue recovery agent.

Terebin oil contains chemicals such as α-pinene, β-pinene, campene, dipentene, limonene, terpinolene, formic acid, acetic acid, resins, etc.In the private sector, pharmaceuticals such as induction stimulants, paints, varnishes, lacquers, etc. Bordeaux terpentin extracted from Pinus pinaster, a kind of pine family, was used as a raw material for paint manufacturing materials, solvents for emulsification, bitumen, synthetic camphor and Borneol terpineol. The remainder is called galipot, which was once used as medical plaster.

In the process of screening an effective material for skin whitening among various natural substances in nature, the present inventors seek to find a substance that is effective for skin whitening and improvement. It confirmed that it represents, and completed this invention.

Therefore, the main object of the present invention is to provide plant essential oils extracted from leaf, lemon, fennel and pine, which effectively inhibit tyrosinase activity, which is an enzyme that plays a key role in melanin biosynthesis, and inhibits melanin biosynthesis of melanoma cells. It is to provide a composition for skin whitening containing.

According to one aspect of the present invention, the present invention provides a composition for skin whitening containing as an active ingredient plant essential oils extracted from at least one plant selected from the group consisting of a leaf, lemon, fennel and pine.

The leaf oil, lemon, fennel and pine essential oils contained as an active ingredient of the present invention have been proved to have various effects in folk medicine or herbal medicine, but studies on skin whitening are insufficient. The inventors have found for the first time that these plant essential oils have an excellent effect on skin whitening and in particular their essential oils have an excellent skin whitening effect.

In the present invention, the method for extracting essential oil components from leaf, lemon, fennel and pine may use a conventional plant essential oil extraction method, such as compression, solvent extraction, simple distillation, reduced pressure distillation or supercritical extraction. It is not limited to one way.

An example of a specific manufacturing method using the conventional plant essential oil extraction method, extracting by adding a leaf, lemon, fennel or dried pine (or rosin) or its pulverized product to the extraction solvent and boiled for 1-20 hours while stirring if necessary After filtration, cooling, concentration, etc., essential oil components can be obtained. The solvent used for the extraction may be hexane, purified water, ethanol, methanol, propanol, butanol, glycerin, butylene glycol, propylene glycol and the like. Preferably, volatile organic solvents such as hexane, petroleum ether, cyclohexane, ethyl acetate and dichloromethane are used. It is preferable to use the quantity of the solvent used for extraction normally 2 to 20 times (weight ratio) of extraction raw material.

In the composition of the present invention, the plant essential oil may be a plant essential oil extracted from a leafy leaf, lemon, fennel or pine, but preferably may be a mixed essential oil of leafy leaf, lemon and fennel, more preferably leafy leaf, lemon, fennel or pine It is characterized by a mixed essential oil. Mixing essential oils of the plants is preferably mixed in the same ratio of leaf oil, renom, fennel and pine essential oil.

In the embodiment of the present invention, the skin whitening effect using these plant essential oils alone showed skin whitening effect through the inhibition of tyrosinase activity in all plant essential oils, in particular, mixed essential oils of leafy, lemon, fennel or pine It shows the highest skin whitening effect, showing synergistic effects in these mixed essential oils (see Table 1 and Table 3).

The skin lightening effect can be achieved by inhibiting tyrosinase activity and melanin production. In the embodiment of the present invention, it was confirmed that the plant essential oil extracted from the leaf, lemon, fennel or pine has excellent effects on inhibiting tyrosinase activity (see Table 1) and melanin production (see Table 3), and using the skin It has been demonstrated that it can be used as a composition having a whitening effect.

In the composition of the present invention, the essential oil components of the leaf, lemon, fennel and pine are already used as a herbal medicine as an extract of natural plants and through the cytotoxicity measurement of the present invention (Experimental Example 2) as confirmed that there is no harmful action However, the content of the composition is not particularly limited, but may be preferably contained in an amount of 0.01 to 50% by weight, more preferably 0.01 to 10% by weight based on the total weight of the composition.

The composition comprising the essential oil components of leaf, lemon, fennel and pine of the present invention can be used as a cosmetic composition and a pharmaceutical composition for skin whitening effect.

When the composition of the present invention is used as a cosmetic composition, in addition to the plant essential oil components as an active ingredient, it includes components commonly used in cosmetic compositions, and can be used in various ways such as cosmetics and face washes having a skin whitening effect. Examples of products to which the composition of the present invention can be added include cosmetics such as various creams, lotions, skins, and the like, and cleansing agents, face washes, soaps, treatments, and essences.

The cosmetic composition of the present invention may further include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extracts.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) Alpha-tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl laurate, and the derivatives thereof (palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetate, Ether, etc.) are also included in the usable vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, the cosmetic composition of this invention can also mix | blend the other component normally mix | blended with a cosmetic composition as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid , Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecate oleate, octylate decyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2 -Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicacapate, capric acid Propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylic acid glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, neopentane dodecyl Isostearyl octanoate, octyl isononate, hexyl decyl neodecanoate, octyl dodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-stealo Esters such as monohydroxystearic acid isostearyl; and the like.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hydroxy Ci-5-methylphenyl) benzotriazole and the like.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to a total weight, More Preferably 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the compound as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like. Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, when the composition of the present invention is used as a pharmaceutical composition, it contains 0.01 to 50% by weight based on the total weight of the composition of the essential oil components extracted from the leaves, lemon, fennel and pine as active ingredients.

The pharmaceutical compositions of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

The pharmaceutical compositions of the present invention can be used in the form of external preparations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, and sterile injectable solutions, respectively, according to a conventional method, preferably It can be used in the form of creams, gels, patches, sprays, ointments, warnings, lotions, linings, pastas or cataplasmas.

Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, oligosaccharides, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

In the case of formulation, it is preferable to use diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants that are commonly used.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are preferably mixed. In addition to simple excipients, lubricants such as magnesium styrate talc may also be used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories, and the like. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The composition for skin whitening according to the present invention is a pharmaceutical composition in the form of an external preparation of a skin, a cream, a gel, a patch, a spray, an ointment, a warning, a lotion, a liniment, a pasta or a cataplasm, as a formulation that can be applied to the skin. It may be prepared by using the composition, but is not limited thereto.

The preferred dosage of the composition containing the plant essential oil component of the present invention as an active ingredient depends on the condition and weight of the patient, the extent of the disease, the drug form, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the desired effect, the composition of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably at 0.001 to 10 mg / kg. Topical administration may be administered once a day or may be divided several times. The dose is not intended to limit the scope of the invention in any way.

As described above, plant essential oils extracted from leaf, lemon, fennel and pine effectively inhibit tyrosinase activity, an enzyme that plays a key role in the melanin biosynthesis process, and inhibit the melanin biosynthesis of melanoma cells. It can be usefully used as a composition and cosmetic composition.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and the scope of the present invention is not to be construed as being limited by these examples.

Example  One. Leaf oil (1)  Produce

200 g of dried dried leaves (Gyeongdong Market, Seoul) were added to 1 kg of hexane, boiled for 3 hours in an extractor equipped with a cooling condenser, filtered through a 400 mesh filter cloth, cooled to room temperature, and concentrated under reduced pressure at 60 ° C using a rotary evaporator. 4.2 g of a yellow essential oil component was obtained. After adding 1 weight% of activated carbon to the obtained essential oil component, after stirring for 1 hour at room temperature, it filtered and obtained 3 g of the off-white refined leaf oil (1).

Example  2. Fennel oil  Produce

200 g of dried fennel (purchased in Kyungdong Market, Seoul) was added to 1 kg of hexane, boiled for 3 hours in an extractor equipped with a cooling condenser, filtered through a 400 mesh filter cloth, cooled to room temperature, and concentrated under reduced pressure at 60 ° C using a rotary evaporator. 5.2 g of a yellow essential oil component was obtained. After adding 1 weight% of activated carbon to the obtained essential oil component, after stirring for 1 hour at room temperature, it filtered and obtained 4.1 g of the off-white refined fennel oil.

Example  3. Preparation of Lemon Oil

200 g of completely dried lemon was added to 1 kg of hexane, boiled for 3 hours in an extractor equipped with a cooling condenser, filtered through a 400 mesh filter cloth, cooled to room temperature, and concentrated under reduced pressure at 60 ° C. using a rotary evaporator to give a light white essential oil 2.2 g. Got.

Example  4. Love Of mixed essential oils of lemon, lemon, fennel

200 g each of dried leaf, lemon, and fennel (purchased from Kyungdong Market, Seoul) were added to 3 kg of hexane, boiled for 3 hours in an extractor equipped with a cooling condenser, filtered through a 400 mesh filter cloth, cooled to room temperature, and then rotated with a rotary evaporator. Concentration under reduced pressure at 60 ° C. yielded 10.2 g of a yellow essential oil component. After adding 1 weight% of activated carbon to the obtained essential oil component, after stirring for 1 hour at room temperature, it filtered and obtained 9.2 g of off-white refined essential oils.

Example  5. Turpentine  Produce

200 g of rosin was added to 1 kg of purified water, mixed well, and boiled and distilled for 5 hours in a distillation with a cooling condenser to obtain 21.2 g of essential oil (terebin oil). 1 wt% of activated carbon was added to the obtained turpentine oil, followed by stirring for 1 hour, followed by filtration to obtain 18.9 g of purified turpentine oil having improved odor.

Example  6. Of needle oil (2)  Produce

1 kg of completely dried leaflets were added to 2 kg of purified water, mixed well, and then boiled and distilled for 5 hours in a still with a cooling condenser to obtain 11.3 g of upper leaf oil. After adding 1% by weight of activated carbon to the obtained leaf oil, the mixture was stirred for 1 hour and filtered to obtain 9.7 g of refined leaf oil (2) having improved odor.

Example  7. Love Essential oils of lemon, lemon, fennel and rosin

Four plant-mixed essential oils were prepared by mixing the plant essential oils of the leaflets, lemons, fennel and rosin, respectively, in a ratio of 1: 1: 1: 1, prepared by the methods disclosed in Examples 1 to 3 and 5.

Example  8. Of leaf oil (3)  Produce

After checking the optimum temperature and pressure conditions suitable for supercritical carbon dioxide extraction for extracting the petrol oil from the petals with high purity (temperature 40 ~ 100 ℃, pressure 3000 ~ 7000 psi), the supercritical carbon dioxide extractor without common solvent Essential oils were extracted. 2.5 g of the dead oil (3) were obtained from 100 g of the fully dried leaf.

Experimental Example  One. Tyrosinase  Activity inhibitory effect

Tyrosinase activity inhibition effect was measured for the essential oil components obtained in Examples 1 to 8.

1) Experiment Method

Inhibitory activity of tyrosinase is generally determined by spectroscopic methods. In this experiment was performed by Barney et al. (Vanni A. at al., Annali di Chimica, 80 (35) , 1990). Tyrosinase is an enzyme purified from potatoes and mushrooms. In this experiment, one extracted from mushrooms was used. After mixing 1.0 ml of 0.1 M potassium phosphate buffer (pH 6.8), 0.3 ml of 0.3 mg / ml L-tyrosine solution and 0.1 ml of 1250 unit / ml mushroom tyrosinase (sigma, USA), sample solutions were added to 0.2 ml each was added for enzymatic reaction at 37 ° C. for 10 minutes. Then, the absorbance of the reaction solution was measured at 480 nm (JASCO V-550, japan) and calculated according to the following Experimental formula 1 to obtain the degree of enzyme inhibitory activity of the sample. As a positive control group, 0.2 ml of arbutin (Bioland Co., Ltd.) of the same concentration was used as the control group, and a reaction solution without adding a sample was used as a control group. The results are shown in Table 1.

[Equation 1]

Tyrosinase inhibition rate (%) = [(A-B) / A] × 100

A: Absorbance at 480 nm of reaction solution without sample

B: absorbance at 480 nm of reaction solution to which sample was added

2) Experimental results

From the above results, it can be seen that the essential oils of the present invention all inhibit tyrosinase and have a whitening effect. In addition, the mixed essential oil extracted from the leaves, lemon and fennel (Example 4) had a suppression rate of 40.2%, and the expected value when each plant essential oil was used alone (the arithmetic mean value of the single plant essential oil was (31.2 + 28.7 + 37.2) / 3 = Expected to be 32.4%). In particular, the mixed essential oil (Example 7) extracted from leaf leaf, lemon, fennel and terevin oil had an inhibition rate of 42.3%, and the expected value when each plant essential oil was used alone (the arithmetic mean value of the single plant essential oil was (31.2 + 28.7 + 37.2). +32.8) / 4 = expected 32.48%) showed a significantly higher synergistic effect, as well as a good effect similar to the arbutin used as a control. Therefore, the essential oils of the present invention can be seen that the tyrosinase inhibitory effect.

Experimental Example  2.B16-F1 Melanoma  Cytotoxicity Measurements Using Cells

Cell viability of B16-F1 melanoma cells (CRL-6323, ATCC) was measured using essential oils extracted from leaf, lemon, fennel and pine.

1) Experiment Method

The present invention was carried out MTT experiments for measuring cytotoxicity, specific experimental methods are as follows.

B16-F1 melanoma cells were inoculated into 6 well plates (Nunc) at a density of 1 × 10 ⁵ cells in DMEM (Dulbecco's modified eagle's medium, Gibco) with 10% FBS (Fetal bovine serum, Gibco) % CO _2, and incubated at 37 ℃. Then, after replacing with fresh DMEM containing 10% FBS, the sample was incubated for 3 days by treating the sample at a concentration of 100 μg / ml. After 3 days the medium was removed and treated with 2 ml of 0.25 mg / ml MTT ((3- [4,5-dimethylthiazole-2-yl] -2,5-diphenyltetrazolium bromide), Sigma) solution for 4 hours at 37 ° C. Reacted. Thereafter, 2 ml of DMSO (dimethylsulfoxide) was added to the cells from which the MTT solution was removed to dissolve MTT formazan and measured by absorbance at 570 nm. All experiments were repeated three times to obtain an average value by statistical treatment, the results are shown in Table 2.

[Equation 2]

Cell viability (%) = absorbance 570 (sample) / absorbance 570 (control) * 100

2) Experimental results

As shown in Table 2, the cell survival rate is 90% or more, it can be seen that essential oils do not exhibit cytotoxicity.

Experimental Example  3.B16-F1 Melanoma  Measurement of Melanin Biosynthesis Inhibitory Effect Using Cells

1) Experiment Method

Melanogenesis inhibition effect using B16-F1 melanoma cells was measured by modifying the method of Fukuda et al. (J. Soc. Cosmetic Chem., 42 (361) , 1991). Well counts according to all concentrations were prepared in triplicate.

Specifically, B16-F1 melanoma cells were inoculated in 6well plates at a density of 1 × 10 ⁵ cells in DMEM with 10% FBS and incubated at 5% CO ₂ , 37 ° C. for one day. Then, after replacing with fresh DMEM containing 10% FBS, each sample was incubated for 3 days by processing 100 μg / ml. After 3 days, the cells from which the medium was removed were washed with 2 ml of PBS (phosphate buffer, saline) and treated with 0.5 ml of 1N NaOH to collect cells into 1.5 ml tubes to obtain intracellular melanin. The collected cells were transferred to a 96well plate, and absorbance was measured at 450 nm to determine the amount of melanin per protein. Arbutin was used as a control of the experimental group. Table 3 shows the results of inhibiting melanin production of essential oils obtained therefrom.

[Equation 3]

Melanin inhibitory effect (%) = [1-MCa / CVa] × 100

CVa: Cell viability of the reaction solution treated with the sample

MCa: [(A-B) / A] × 100

A: absorbance at 450 nm of untreated sample solution

B: absorbance at 450 nm of the reaction solution treated with the sample

2) Experimental results

As shown in Table 3, the essential oil components of the present invention have a melanin production inhibitory effect.

From this, it can be seen that the essential oil components extracted from the leaves, lemon, fennel and pine (Sinjin) all have a whitening effect through the inhibition of melanin production. In addition, the mixed essential oil extracted from the leaves, lemon and fennel (Example 4) had a suppression rate of 26.14%, and the expected value when each plant essential oil was used alone (the arithmetic mean value of the single plant essential oil was (13.82 + 19.58 + 9.11) / 3 = Expected 14.17%). In particular, the mixed essential oil (Example 7) extracted from leaf leaf, lemon, fennel and terevin oil had an inhibition rate of 33.67%, and the expected value when each plant essential oil was used alone (the arithmetic mean value of single plant essential oil was (13.82 + 19.58 + 9.11). +23.56) / 4 = 16.5% expected) was not only a significantly higher synergistic effect, but also significantly higher than the arbutin used as a control.

Formulation example  1. flexible lotion ( skin )

As described below, the soft leaf essential oil obtained in Examples 1 and 7 and a flexible lotion (skin) containing mixed essential oils of leaf, lemon, fennel and rosin were prepared according to a conventional method.

Formulation example  2. Nourishing Cosmetic (Lotion)

As described below, nutrient essential oil (lotion) containing fennel essential oils obtained in Examples 2 and 7 and mixed essential oils of rosemary, lemon, fennel and rosin were prepared according to a conventional method.

Formulation example  3. Nourishing Cream

As described below, a nourishing cream containing lemon essential oils obtained in Examples 3 and 7 and mixed essential oils of rosemary, lemon, fennel and rosin were prepared according to a conventional method.

Formulation example  4. Essence

As described below, essences containing the rosin essential oils obtained in Examples 5 and 7 and mixed essential oils of rosemary, lemon, fennel and rosin were prepared according to a conventional method.

Formulation example  5. Foundation

As described below, a foundation containing the essential oils obtained in Examples 6 and 7 and mixed essential oils of the petals, lemon, fennel and rosin were prepared according to a conventional method.

Formulation example  6. Hydrophilic Ointment

As follows, the hydrophilic ointment containing the essential oils obtained in Examples 8 and 7 and mixed essential oils of the leaves, lemon, fennel and rosin were prepared according to a conventional method.

Claims (9)

Skin whitening composition containing as an active ingredient plant essential oils extracted from at least one plant selected from the group consisting of leafy, lemon, fennel and pine.
The composition of claim 1, wherein the plant essential oil is a mixed essential oil of leafy, lemon and fennel.
The composition of claim 1, wherein the plant essential oil is a mixed essential oil of leafy, lemon, fennel and pine.
The composition of claim 1, wherein the plant essential oil has a skin lightening effect by inhibiting tyrosinase activity and melanin production.
The composition of claim 1, wherein the plant essential oil is included in an amount of 0.01 to 50 wt% based on the total weight of the composition.
The composition of claim 1, wherein the composition is a cosmetic composition.
The method of claim 6, wherein the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence , Pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and a body cleanser, characterized in that it has a formulation selected from the group consisting of.
The composition of claim 1, wherein the composition is a pharmaceutical composition.
9. The composition of claim 8, wherein the pharmaceutical composition has one formulation selected from the group consisting of creams, gels, patches, sprays, ointments, warnings, lotions, linings, pasta and cataplasma. .