KR100549661B1 - Cosmetic Composition having Anti-oxidative and Anti-inflammatory Activity - Google Patents

Abstract

The present invention relates to a cosmetic composition having a good antioxidant and anti-inflammatory effect and a method for producing the same.

The present invention relates to a cosmetic composition comprising vitamin C and one or more herbal extracts selected from the group consisting of green tea, phosphorus mugwort and pine needles, the composition particularly having cyclooxygen, which not only has excellent antioxidant activity against free radicals but also causes inflammation Since it has an anti-inflammatory activity that inhibits Cyclooxygenase, it can be used as a natural functional cosmetic composition.

Antioxidant, anti-inflammatory, green tea, phosphorus mugwort, pine needles, vitamin C, cyclooxygenase, cosmetic composition.

Description

Cosmetic composition having an antioxidant and anti-inflammatory effect {Cosmetic Composition having Anti-oxidative and Anti-inflammatory Activity}

1 is a diagram showing the lipid peroxidation inhibitory effect of the functional composition and each positive control group having the antioxidant and anti-inflammatory effects of the present invention.

The present invention relates to a functional cosmetic composition comprising a water-soluble extract of green tea, phosphorus mugwort and pine needles and vitamin C having an antioxidant and anti-inflammatory effect, and more particularly, the composition has an antioxidant effect and inflammation that eliminates free radicals. The present invention relates to a functional cosmetic composition having an anti-inflammatory effect of inhibiting cyclooxygenase (Cyclooxygenase EC 1.14.99.1; hereinafter COX).

All aerobic organisms, including humans, are equipped with self-defense mechanisms to protect against free radicals that always occur during oxygen-based energy metabolism. Arthritis, circulatory disorders, as well as a number of diseases such as dementia (Halliwell et al., Drugs , 42 , pp569-605, 1991; Fukuzawa et al., J. Act.oxyg.Free Rad , 1 , pp55) -70, 1990).

La commonly-radical called the active oxygen is most of triplet oxygen stable form of oxygen ⁽³ O ₂₎ is the superoxide anion singlet oxygen that is generated is reduced in oxidation-reduction process _{^{(Superoxide anion; 1 O 2 -}} ) and Unpaired free radicals, such as hydrogen peroxide (H ₂ O ₂ ) and hydroxy radicals (.OH), which cause diseases by damaging factors of the immune system such as proteins, DNA, enzymes and T cells (Regnstrom et al. al., Lancet , 16 , pp 1183, 1992; Gey et al., Am. Ac. J. Cin. Nutr , 53 , pp326, 1991).

For this reason, researches on the development of antioxidants have been actively conducted, and antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase, which are enzyme-protective antioxidants, and vitamins, which are natural antioxidants, E, vitamin C, carotenoids, glutathione, and the synthetic antioxidant t-Butyl-4-hydroxyanisole (BHA) dibutylhydroxytoluene (3,5- (t-Butyl)- Many antioxidants, such as 4-hydroxytoluene (BHT), are known, but antioxidant enzymes decrease their protection against free radicals as they age, while synthetic antioxidants are safer and more potent because their mutagenicity and toxicity are noted. The development of natural antioxidants is urgently required (Hatano et al., Natural Medicines , 49 , pp 359-363; Masaki et al., Biol. Pharm. Bull , 18 , pp 162-166, 1995).

Inflammation refers to a pathological condition of an abscess formed by invasion of external bacteria, and an inflammatory response is a biological defense reaction process for repairing and repairing damage caused by an invasion that causes some organic changes in cells or tissues of a living body. COX, an enzyme that causes these inflammations, has two types of isoforms. COX-1 is a substance that contains prostaglandin (HG) in the organs and tissues of the human body, such as the gastrointestinal tract or kidneys. It is also involved in creating. However, COX-2 is known to act only on the site of inflammation. Non-steroidal anti-inflammatory drugs (NSAIDs), which are commercially available on the market, inhibit both COX-1 and COX-2 at the same time, or mainly COX-1. It is known to cause many side effects by simultaneously inhibiting HG which is essential for maintaining function of time, gastrointestinal tract or kidney (Isselbcher et al., Harrison's Principles of Internal Medicine , (13th ed) 2 , pp1543-1711). Therefore, recently developed selective COX-2 inhibitors can significantly reduce the side effects while maintaining the existing anti-inflammatory analgesic effect, the use of which is currently increasing, and the side effects are less There is a need for the development of selective COX-2 inhibitors.

On the other hand, Camellia sinensis belongs to theaceae, the young and soft leaves of the tea tree mainly contains purine alkaloids and caffeine, the content of the content is 1 to 5%. In addition to its trace amounts, it contains Theobromine, Theophylline and Xanthine. Tannin in tea leaves is a catechol (Galloyl- l -epigallocatechol) mainly -ℓ- epi go galloyl, which is contained together with caffeine. The pharmacological action of tea leaves is mainly caused by xanthine derivatives (caffeine and theophylline), and also contains a large amount of tannic acid, thus acting as antibacterial and vitamin P. Pharmacological action is to excite the central nervous system, promote mental function, increase thinking ability, eliminate fatigue, expand coronary blood vessels, and diuretic (Jung Bo-seop and Shin Min-kyo, Ph.D., pp403-405, Younglimsa, 1998) . The young leaves of the tea tree can produce various kinds of teas with different flavors depending on the processing method. For example, the tea leaves, black tea, oolong tea, Iron ore, cannon tea, green tea, etc. can be manufactured.

Injin mugwort (Artemisia capillaris Thunb) is native to various parts of Korea and its young stems and leaves are called injinho. Scoparone, chlorogenic acid, and capillin are known (Bo Bo-seop and Shin Min-kyo, Ph.D., pp1016-1017, Younglimsa, 1998).

The inventors of the present invention screened natural products with excellent antioxidant effects, while green tea containing a large amount of (-)-catechin ((-)-Catechin) and (-)-epigallocatechin gallate (-) In addition, when the combination of phosphorus mugwort, pine needles and vitamin C containing a large amount of antioxidant substances, it was found that it can be applied to a functional cosmetic composition having excellent antioxidant and anti-inflammatory effects, to complete the present invention based on this.

In order to achieve the above object, the present invention provides a functional cosmetic composition having an anti-inflammatory effect as well as an antioxidant effect, characterized in that the green tea, phosphorus mugwort and pine needle water-soluble extract and vitamin C.

In order to achieve the above object, the present invention provides a functional cosmetic composition essentially containing one or more plant extracts and vitamin C selected from the group consisting of green tea, jinjin mugwort and pine needles.

Specifically, the composition of the present invention is 20-75% by weight of green tea water-soluble extract, 10-60% by weight of water extract of pine needles, 10-60% by weight of pine needle water-soluble extract, preferably 40-70% by weight of water-soluble water extract of green tea, 14-40% by weight, pine needle water-soluble extract 14-40% by weight of the plant extract having a composition ratio and 2-10% by weight of vitamin C.

The composition of the present invention may be prepared with a composition comprising vitamin C and one or more plant extracts selected from the group consisting of buttercup, spiny ginseng, Chinese quince, fish vinegar and ginger in addition to the plant extract.

The present invention has an antioxidant and anti-inflammatory effect made by combining green tea, phosphorus mugwort or pine needles with hot water extracted at 80 to 110 ℃ for 1 to 5 hours, concentrated to a solid content of 10 to 40%, and then dried dry concentrate and vitamin C. It provides a functional cosmetic composition.

The cosmetic composition may be included in an amount of 0.001% to 30% by weight based on the total weight of the cosmetic.

Hereinafter, the present invention will be described in detail.

In order to prepare green tea, jinjin mugwort and pine needle extract used in the present invention,

Specifically, after washing the green tea, gingi wormwood or pine needles, dried in the shade and crushed or pulverized, about 1 to 30 times the weight (kg) of water, lower alcohols such as methanol, ethanol or a mixed solvent thereof , Preferably from 1 to 10 times, preferably from 2 times to 20 to 120 ° C., preferably from 100 to 110 ° C. with water for about 1 to 2 days, preferably about 2 to 12 hours Extraction method such as hot water extraction, ultrasonic extraction, reflux cooling extraction, etc. 5 times repeatedly, more preferably by soluble extract according to water, lower alcohol or a mixed solvent thereof by 80 to 110 ℃ hot water extraction method 1 to 5 hours Crude extract is obtained, filtered and concentrated under reduced pressure or vacuum to obtain an extract concentrate, which is additionally freeze-dried to obtain a powder form.

Extracts obtained by the above production method can be used in the cosmetic composition of the present invention, it can also be purchased and used, the extract can be used in the present composition in the form of a high concentration of 20 to 90% or powder form.

The composition of the present invention is composed of 40-60% by weight of green tea water-soluble extract, 17.5-25% by weight water extract of ginko jinja, 17.5-25% by weight of pine needle water extract and 5-10% by weight of vitamin C.

The present invention provides a cosmetic composition useful for the prevention, amelioration or treatment of dermatitis diseases in which the amount of the extract has 0.01 to 30% by weight, preferably 0.01 to 5.0% by weight of the total cosmetic composition, and includes cleansing, face wash, soap, In addition to shampoos, rinses and treatments, there is provided a new use of cosmetic compositions useful for the prevention, amelioration or treatment of dermatitis.

The composition of the present invention can be used in a variety of cosmetics, face wash and shampoo for the prevention, improvement or treatment effect of dermatitis diseases. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, and skins, and shampoos, rinses, cleansing agents, face washes, soaps, treatments, and cosmetic liquids.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be formulated in cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-α-tocopherol, d-α-tocopherol, d-δ-tocopherol) and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-α-tocopherol acetate, dl-α-tocopherolvitamin E nicotinic acid, DL-pantothenyl alcohol, D-pantothenyl) Alcohols, pantotenylethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, pit spingosine, sphingolipid lipid and the like can be given. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isetyl, isostyl acid mystearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacinate, adidi Diisopropyl phosphate, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl stearate trimethylol propane, tetra 2-ethylhexanopentane Tol, cetyl caprylate, lauric acid decyl, lauric acid hexyl, myristic acid decyl, myristic acid myristyl, myristic acid cetyl, stearyl stearate, decyl oleate, lisinooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoic acid cetostearyl, 2-ethylhexanoic acid stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Isostearic acid octyldecyl, polyglycerol oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myritic lactic acid, cetyl lactate, octyl lactate, trit citrate Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethyl hexyl succinate, diisobutyl adipic acid, diisopropyl sebacic acid, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymer, dimethylsiloxane and methyl steoxysiloxane copolymer and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol (polymerization degree n = 2 or more), polypropylene glycol (Polymerization degree n = 2 or more), polyglycerol (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin Fatty Acid Ester, POE Alkyl Ether, POE Fatty Acid Ester, POE Cured Castor Oil, POE Castor Oil, POE · POP (Polyoxyethylene Polyoxypropylene) Copolymer, POE / POP Alkyl Ether, Polyether Modified Silicone, Laurin Acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

As anionic surfactant, fatty acid soap, (alpha)-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphorus salt, alkylamide Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, carbon black and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-β-alanine calcium, N-lauroyl-β-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids, such as N (epsilon) -lauroyl-L- lysine, N (epsilon) -palmitolysine, N (alpha)-partoylol nitin, N (alpha)-lauroyl arginine, and N (alpha) -cured fatty acid acyl arginine; N-acyl polypeptides, such as N-lauroyl glycyl glycine; α-amino fatty acids such as α-aminocaprylic acid and α-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate and cinnamic acid benzyl , Paramethoxy cinnamic acid 2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono 2-ethylhexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, urokanoic acid Ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonate, dihydroxybenzophenone, tetra Hydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1,3 , 5-triazine, 2- (2-hydric When the like can be mentioned 5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to gross weight, More Preferably it is 0.01-3 weight% mix.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Examples of the form of the cosmetic composition are not particularly limited, and examples thereof include milky lotion, cream, lotion, pack, foundation, lotion, beauty liquid, hair cosmetic, and soap.

Specific examples of the cosmetic of the present invention face wash, face wash foam, cleansing cream, cleansing milk, cleansing lotion, massage cream, cold cream, moisturizing cream, emulsion, lotion, pack, aftershave cream, sunburn prevention cream, suntan oil, soap , Body shampoo, hair shampoo, hair rinse, hair treatment, wool, hair care, hair cream, hair liquid, set lotion, hair spray, hair bridge, coloring, color spray, permanent wave liquid, press powder, loose powder Eye shadows, hand creams, lipsticks, and the like.

In addition to the extract, the cosmetic of the present invention comprises a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extracts (such as blending components which may be added in addition to those exemplified above if necessary). It can obtain by preparing according to a well-known method, for example, the method described in a "transdermal application preparation development manual" 1st edition of Matsumotomichio supervision (Issuyoshi Seishin 1985).

In addition, the present invention provides a cosmetic additive for the prevention, amelioration or treatment of skin inflammatory diseases containing green tea, jinjin mugwort, pine needle extract and vitamin C.

The cosmetic additives can be used in the production process or finished products such as existing cosmetics and cleansing agents, and have a function to prevent, improve or treat allergic skin diseases or non-allergic dermatitis diseases.

It can also be used in creams, lotions, massage packs, systemic cleansers, soaps, shampoos, and other care preparations.

Since the plant extract of the present invention has little toxicity and side effects, it can be used safely even for long-term use for the purpose of prevention.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following Examples and Experimental Examples are only illustrative of the present invention, and the content of the present invention is not limited to the following Examples and Experimental Examples.

Example 1 Preparation of Essential Plant Extracts

 30 g each of green tea, pine needles, and jinjin mugwort purchased from Nonglim Herbal Medicine Co., Ltd. were put in 600 ml of water and extracted with hot water at 110 ° C for 3 hours to obtain hot water extract, which was then concentrated in a vacuum concentrator until the solid content reached 30%. The concentrate was lyophilized to give 2.5, 2.3 and 2.8 g of dried green tea, pine needles and dried jinjinji powder, respectively.

Example 2. Other Plant Extract Preparation Example

  Each of 30 g of buttercups, spiny ginseng, Chinese quince, fish vinegar, ginger and onion were prepared in the same manner as in the production process described in Example 1 to prepare 2.1, 1.8, 2.5, 2.8, 3.0 and 2.5 g of the dry powder, respectively.

Example 3 Cosmetic Composition Composition Example 1

Green tea, Injin mugwort, pine needle water-soluble extract and Vitamin C (Roche) prepared in Example 1 were formulated in 60%, 17.5%, 17.5% and 5% based on the solid content% to prepare a cosmetic composition 1 of the present invention It was.

Example 4 Cosmetic Composition Preparation Example 2

Green tea, Injin mugwort, pine needle water-soluble extract and vitamin C prepared in Example 1 were formulated into 55%, 15%, 35% and 5%, respectively, based on the solids content% to prepare a cosmetic composition 2 of the present invention.

Experimental Example 1. Antioxidative effect experiment of each plant extract and vitamin C of the present invention

In order to confirm the antioxidant efficacy of the plant extracts of Example 1 and Example 2 and vitamin C used in the present invention, the free radical 1,1-diphenyl-2-picrylhydrazyl (1,1-Diphenyl-2 -picrylhydrazyl (hereinafter DPPH) was used.

DPPH was used by dissolving in ethanol to 2 × 10 ^-4 M concentration. After 1.5 mL of DPPH, 0.15 mL of extract sample (1000 ppm-30 ppm) or vitamin C (1000 ppm-30 ppm) of Example 1 or 2 were added thereto and 1.35 mL of distilled water, respectively, the reaction was carried out at room temperature for 30 minutes, Absorbance was measured at 520 nm. For DPPH scavenging effect, IC ₅₀ (50% Inhibitory activity) was compared with respect to a sample having a DPPH radical scavenging activity value of 50% or more calculated using Equation 1 below.

DPPH radical scavenging activity (%)

= ((A _control -B _control )-(A _sample -B _sample )) / (A _control -B _control ) × 100

A _control : Absorbance of control group without sample

A _sample : Absorbance of reaction group to which sample is added

B _control : Absorbance of control group without DPPH

B _sample : Absorbance of reaction group without DPPH

As a result, Table 1 below shows the scavenging effect of free radicals using DPPH at 1000 ppm concentrations of nine plant extracts and vitamin C (Roche, Switzerland), respectively, and Table 2 below shows free radical scavenging using DDPH. The antioxidant effects of green tea, phosphorus mugwort, pine needles and vitamin C, which are excellent in effects, are expressed as IC ₅₀ values.

DPPH radical scavenging activity (%) green tea 95.03 Injin mugwort 85.43 pine needles 66.05 Buttercup 34.19 Go away 38.35 Quince 43.60 Eoseongcho 24.87 ginger 44.22 onion 41.23 Vitamin c 95.93

DPPH radical scavenging activity (IC ₅₀ ppm (μM) green tea Injin mugwort pine needles Vitamin c 5.46 22.50 35.96 3.86 (21.93)

Experimental Example 2 Antioxidant Effect Experiment of Functional Cosmetic Composition of the Present Invention

With reference to the results obtained in Experiment 1, the functional cosmetic composition of Example 3 containing green tea, jinjin mugwort, pine needle water-soluble extract and vitamin C, and vitamin C, Pycnogenol (Pycnogenol; Horphag, Switzerland), Vitamin E as a positive control (Sigma, USA, T-3251), (±) -catechin ((±) -Catechin; Sigma, USA, C-1788), beta-carotene (β-Carotene; Sigma, USA, C-4582) , Astaxanthin (Astaxanthin; Sigma, USA, A-9335) was compared with the free radical scavenging effect using the DPPH in the same manner as above to obtain an IC ₅₀ value.

As a result, (±) -catechin showed the best DPPH radical scavenging effect and the functional composition of Example 3 of the present invention showed better effect than the existing vitamin E, beta-carotene, astaxanthin. (See Table 3).

DPPH radical scavenging activity (IC ₅₀ ppm (μM) Functional composition of Example 3 Vitamin c Pycnogenol Vitamin E (±) -catechin β-carotene Astaxanthin 6.89 3.86 (21.93) 6.38 9.97 (23.14) 3.24 (11.17) 〉 50 (〉 93.12) 8.83 (14.79)

Experimental Example 3. Superoxide anion radical scavenging effect

Superoxide anion radical scavenging effect measurement was used by modifying the method of Superoxide dismutase (SOD) activity detection kit (Wako, Japan, 435-70601).

0.05 ml each of the functional composition of Example 3 or each sample of the positive control group (500-30 ppm), color developing solution (0.4 mM Xanthin + 0.1 M Phosphate buffer, pH 8.0) + 0.24 mM nitroblue tetra Add 0.5 ml of Nitroblue tetrazolium (NO ₂ -TB), enzyme solution (0.048 unit / ml Xanthine oxidase, from Butter milk solution + 0.5 ml of 0.1 M phosphate buffer solution (pH 8.0) and mix well After reacting for 20 minutes at 37 ° C., 1 ml of reaction stop solution (69 mM sodium dodecyl sulfate) was added to stop the enzyme reaction and the absorbance was measured at 560 nm. Pycnogenol, vitamin E, (±) -catechin, beta-carotene, astaxanthin was used, the detailed experimental method is shown in Table 4, the superoxide anion radical scavenging effect is calculated as shown in Equation 2 below IC ₅₀ value Compared to Table 5 below It is shown.

Superoxide Anion Radical Scavenging Activity (%)

= ((A _control -B _control )-(A _sample -B _sample )) / (A _control -B _control ) × 100

Bone sword Blank Reaction group Control Reaction group Control sample 0.05 ml (sample) 0.05 ml (solvent in which sample was dissolved) 0.05 ml (sample) 0.05 ml (solvent in which sample was dissolved) Color 0.5 ml 0.5 ml 0.5 ml 0.5 ml Enzyme solution 0.5 ml 0.5 ml - - Buffer - - 0.5 ml 0.5 ml Warm for 20 minutes at 37 ℃ Reaction Stopping Liquid 1.0 ml 1.0 ml 1.0 ml 1.0 ml Shake well and measure absorbance at 560 nm as water Absorbance A _sample A _control B _sample B _control

As can be seen in Table 5, when compared in ppm unit, the functional cosmetic composition of Example 3 showed the best superoxide anion radical scavenging effect, about 2 times than pycnogenol, about 5 times than vitamin C, and astaxanthin The effect was about 17 times higher and vitamin E and beta-carotene showed little scavenging effect.

Superoxide anion ⁽¹ O ₂ ^-) scavenging activity (IC ₅₀ ppm (μM) Functional composition of Example 3 Vitamin c Pycnogenol Vitamin E (±) -catechin β-carotene Astaxanthin 1.17 6.36 (36.13) 2.26 〉 24.39 (〉 56.60) 3.22 (11.09) 〉 24.39 (〉 45.43) 20.48 (34.31)

Experimental Example 4. Measurement of Lipid Peroxidation Inhibition Effect

In order to determine the lipid peroxidation inhibitory ability of the functional composition of the present invention was tested as follows. At this time, the lipid peroxidation inhibitory effect using the Ferric-Thiocyanate (FTC) method is used by applying the experimental method described in Takeshi et al., Takeshi et al, Food Chemistry , 75 , pp237-40, 2001 It was.

0.2 ml of each sample of the functional composition or positive control of Example 3, 0.5 ml of 0.2 M sodium phosphate buffer (pH 7.0), 0.5 ml of 2.5% linoleic acid dissolved in ethanol, 0.1 M 2, 50 μl of 2′-azobis-2-amidinopropane-dihydrochloride (AAPH) was mixed, and then reacted in a dark at 37 ° C. for 100 minutes and 200 minutes. Thereafter, 0.1 ml of the reaction sample solution was added to 9.7 ml of 75% ethanol, 0.1 ml of 30% ammonium thiocyanate, and 20 mM ferric chloride (FeCl ₂ ) dissolved in 3.5% hydrochloric acid solution (HCl) for 3 minutes. After mixing well the absorbance at 500 nm was measured. As a positive control, vitamin C, pycnogenol, vitamin E, (±) -catechin, beta-carotene and astaxanthin were used. Lipid peroxidation inhibitory activity (%) was calculated using the following equation (3).

          Lipid peroxidation inhibitory activity (%)

= ((A _control -B _control )-(A _sample -B _sample )) / (A _control -B _control ) × 100

A _control : Absorbance of control group without sample

A _sample : Absorbance of reaction group to which sample is added

B _control : Absorbance of control group without linoleic acid

B _sample : Absorbance of reaction group without linoleic acid added

As a result, vitamin E showed the best inhibitory effect on fat peroxidation at 100 and 200 minutes, and functional composition showed slightly lower inhibitory effect than vitamin E and (±) -catechin, but showed better inhibitory effect than other positive controls. (See FIG. 1).

Experimental Example 5. Measurement of hydroxy radical scavenging effect

In order to confirm the hydroxy radical scavenging effect of the functional composition of the present invention it was experimented as follows. At this time, the hydroxy radical scavenging effect is to measure the oxidation inhibition rate of 2-deoxyribose due to the hydroxy radical, Takeshi et al., Food Chemistry , pp29-33, 2003) was modified.

First, 150 μl of 10 mM ferrous sulfate (FeSO ₄ ) -EDTA, 150 μl of 10 mM 2-deoxyribose, 300 μl of the functional composition or positive control sample of Example 3, and 0.2 M sodium phosphate buffer solution (Sodium phosphate buffer, pH 7.0) 450 µl, 10 mM hydrogen peroxide (H ₂ O ₂ ) 150 µl, distilled water 300 µl was added. The reaction is started by adding H ₂ O ₂ . After 4 hours of reaction at 37 ° C., 750 μl of 2.8% Trichloroacetic acid and 750 μl of 1.0% Tribarbituric acid were added to stop the reaction. The mixture was boiled and cooled for 10 minutes to measure absorbance at 520 nm. . At this time, vitamin C and pycnogenol dissolved in distilled water were used as positive controls, and samples dissolved in alcohol or dimethyl sulfoxide (DMSO) (vitamin E, (±) -catechin, beta-carotene, astaxanthin). Was less hydroxy radicals than samples dissolved in distilled water and excluded from the positive control. The hydroxy radical scavenging effect was calculated using the following equation.

Hydroxy radical scavenging activity (%)

= ((A _control -B _control )-(A _sample -B _sample )) / (A _control -B _control ) × 100

A _control : Absorbance of control group without sample

A _sample : Absorbance of reaction group to which sample is added

B _control : Absorbance of control group without hydrogen peroxide

B _sample : Absorbance of reaction group without hydrogen peroxide

As a result, as shown in Table 6, vitamin C showed an inhibitory effect of 80% or more even at 200 ppm, the functional composition of Example 3 showed an inhibitory effect about 14% higher than pycnogenol at 2,000 ppm.

Concentration (ppm) Hydroxy radical (.OH) scavenging activity (%) Sample concentration Total reaction sample concentration Functional composition of Example 3 Vitamin c Pycnogenol 10,000 2,000 82.97 96.39 57.64 1,000 200 15.56 85.14 -6.61

Experimental Example 6. Measurement of COX-1, COX-2 enzyme inhibitory effect

In order to determine the inhibitory effect on the COX-1, COX-2 enzymes, which are inflammation inducing enzymes of the functional composition of the present invention, the following experiments were performed. At this time, the cyclooxygenase inhibitory effect is reduced to N, N, N ', N'-tetramethyl- p -phenylenediamine (D) while reducing from prostaglandin G ₂ (PG G ₂ ) to prostaglandin H ₂ (PG H ₂ ). The method described in Chintakunta et al., Eur. J. Med. Chem., 37. pp339-347, 2002 was modified by measuring the oxidation rate of TMPD.

100 mM Tris-HCl buffer (pH 8.0), 3 μM EDTA, 15 μM Hematin, 150 units in 1 mL (COX-1 or COX-2, Sigma) USA, C-0733, C-0858), the functional composition of Example 3 and each of the other samples were mixed at a concentration of 30 pppm-3 ppm and pre-reacted at 25 ° C. for 15 minutes, followed by TMPD and 100 μM arachidonic acid (Aarachidonic acid) was added to measure the initial rate of oxidation of TMPD for 25 seconds at 603 nm, and expressed as IC ₅₀ value using Equation 5 below.

COX-1 or COX-2 inhibitory activity (%)

= ((A _control -B _control )-(A _sample -B _sample )) / (A _control -B _control ) × 100

A _control : Absorbance of control group without sample

A _sample : Absorbance of reaction group to which sample is added

B _control : Absorbance of control group without arachidonic acid

B _sample : Absorbance of reaction group without arachidonic acid

As a result, the inhibitory effect of the inflammatory enzymes on the functional composition was more than 1 / 100-fold (0.01 / 11.67) compared with Celcoxib and Rofecoxib, COX-2 inhibitors with selective COX-2 inhibitory activity. 0.1 / 11.67) and 1 / 4.5 times (2.79 / 11.67) than NSAIDs, Indomethacin. However, the COX-1 inhibitory activity of the functional composition was about 100 times lower than that of indomethacin, and the COX-2 IC ₅₀ / COX-1 IC ₅₀ ratio was about 26 times lower. It is likely to be used for the treatment of inflammation with less side effects due to the tendency to inhibit 2 (see Table 7).

COX inhibitory activity (IC ₅₀ ppm (μM)) IC ₅₀ , Non COX-2 / COX-1 COX-1 COX-2 Functional Composition of Example 3 2.24 11.67 5.209 ^* Celecoship 4.07 (10.7) 0.01 (0.036) 0.002 (0.00336) ^* Lopecoship 〉 157.18 (〉 500) 0.10 (0.321) 0.0006 (0.0006) ^* Indomethacin 0.02 (0.067) 2.79 (7.8) 139.5 (116.4) ^* : Eur. J. Med. Chem 3. 339-347. (2002) Contents

Experimental Example 7. Measurement of PEP enzyme inhibitory effect

In order to confirm the inhibitory effect of the dementia-related enzyme prolyl endopeptidase enzyme (PEP) of the functional composition of the present invention was tested as follows. At this time, the method described in Kimura et al. (Kimura et al., Agri. Biol, Chem., 54 (11) , pp3021-3022, 1990) was used for the measurement of PEP inhibitory effect.

First, 0.84 ml of 0.1 M Tris-HCl (pH 7.0), 0.05 ml of PEP (0.1 unit / ml, Seikagakukougyo, Japan, product No. 120125), the functional composition of Example 3, and 0.01 ml of each sample were mixed, followed by 30 ° C. at 5 minutes before the reaction was 40% 1,4-dioxide acid (Dioxane) was 2 mM Z-Gly-Pro- p NA dissolved in a solution (Seikagakukougyo Co., Japan, product number 451004) 30 ℃ by the addition of 0.1 ㎖ for The reaction was stopped for 30 minutes, and 0.5 ml of reaction stop solution (10 g Triton-X) / 95 ml 1 M Acetate buffer pH 4.0 was added to stop the enzymatic reaction, and then the absorbance was measured at 410 nm. The enzyme inhibitory activity was expressed as an IC ₅₀ value by using Equation 6 below.

PEP Inhibitory Activity (%)

= ((A _control -B _control )-(A _sample -B _sample )) / (A _control -B _control ) × 100

A _control : Absorbance of control group without sample

A _sample : Absorbance of reaction group to which sample is added

B _control : Absorbance of control group without 2 mM Z-Gly-Pro- p NA

B _sample : Absorbance of reaction group without 2 mM Z-Gly-Pro- p NA

The results of the experiment show that, as shown in Table 8, the effect of PEP enzyme inhibition of the functional composition inhibited PEP effect by Staurosporine (Staurosporine, Molecular Weight 466) and Propetine (Molecular Weight 2322), known as PEP inhibitors derived from natural products (Kimura et al., Agri .. Biol. Chem. , 54 (11) , pp 3021-3022, 1990; Kimura et al., The Journal of Antibiotics , 50 , pp373-378, 1997), the inhibition rate of the composition of Example 3 was about 1/13 times the inhibition rate of staurosporin (0.359 / 4.55), rather than the inhibition rate of relatively high molecular weight propeptin. Results with about 1 / 1.8 times (2.55 / 4.55) are shown.

PEP Inhibitory Activity IC ₅₀ ppm (μM) Functional composition of Example 3 ^* Star right cyclosporine ^** Profetin 4.55 0.359 (0.77 μM) 2.55 (1.1 μM) ^* Agri .. Biol. Chem. 54 (11), 3021-3022, 1990. ^** Contents in The Journal of Antibiotics 50, 373-378, 1997.

Hereinafter, an example of the preparation of the functional cosmetic composition will be described, but the present invention is not intended to be limited thereto, but is intended to be described in detail.

Soap manufacturers

Cosmetic composition of Example 3 ........... 1-30 (%)

Maintenance ...

Sodium hydroxide ...............

Sodium Chloride .........................................

Spices Quantity

The whole amount was 100 with purified water, and soap was prepared by the above blending ratio.

Lotion Manufacturing 1

Cosmetic Composition of Example 3 ...... 3.00 (%)

L-ascorbic acid-2-phosphate magnesium salt ......... 1.00

Water Soluble Collagen (1% Aqueous Solution) ............ 1.00

Sodium Citrate ... 0.10

Citric Acid ........................................... 0.05

Licorice Extract ......... 0.20

1,3-butylene glycol ... 3.00

The whole amount was 100 with purified water, and a lotion was prepared by the above blending ratio (%).

Lotion Manufacturing 2

Cosmetic Composition of Example 3 ............ 3.00 (%)

L-ascorbic acid-2-phosphate magnesium salt ......... 1.00

Water Soluble Collagen (1% Solution) ....................... 1.00

Sodium Citrate ... 0.10

Citric Acid ........................................... 0.05

1,3-butylene glycol ... 3.00

The whole amount was 100 with purified water, and a lotion was prepared at the above blending ratio (%).

Lotion Manufacturing 3

Cosmetic composition of Example 3 ....... 4.00 (%)

Brown algae extract ......................................... 1.00

Sodium Citrate ... 0.10

Citric Acid ........................................... 0.05

1,3-butylene glycol ... 3.00

The whole amount was 100 with purified water, and a lotion was prepared at the above blending ratio (%).

Cream manufacturers

Cosmetic composition of Example 3 ...................................... 1.00 (%)

Polyethylene Glycol Monostearate ............... 2.00

Self-emulsifying glycerin monostearate ......................... 5.00

Cetyl Alcohol ... 4.00

Squalene ......................................... 6.00

Glyceryl tri2-ethylhexanoate ......... 6.00

Sphingose Sugar Lipids ......................................... 1.00

1.3-Butylene Glycol ... 7.00

The total amount was 100 with purified water, and a cream was prepared at the blending ratio (%).

Pack manufacturer

Cosmetic composition of Example 3 .................. 5.00 (%)

Polyvinyl Alcohol ... 13.00

L-ascorbic acid-2-phosphate magnesium salt ......... 1.00

Lauroylhydroxyproline ............... 1.00

Water Soluble Collagen (1% Aqueous Solution) ............... 2.00

1,3-butylene glycol ... 3.00

Ethanol ......................................... 5.00

The total amount was 100 with purified water, and a pack was prepared at the blending ratio (%).

Essence

Cosmetic composition of Example 3 ............ 2.00 (%)

Hydroxyethylene Cellulose (2% Aqueous Solution) ... 12.00

Xanthan gum (2% aqueous solution) ....................... 2.00

1,3-butylene glycol ......... 6.00

Dark glycerin ... 4.00

Sodium Hyaluronate (1% Aqueous Solution) ......................... 5.00

The total amount of the purified water was 100, and a cosmetic liquid was prepared at the blending ratio (%).

As described above through the above Examples and Experimental Examples, the cosmetic composition of the present invention exhibited not only an excellent antioxidant effect but also an anti-inflammatory effect, and thus, as a cosmetic composition and a cosmetic additive that can improve skin anti-aging and skin inflammation symptoms. It may be useful.

Claims (8)

A cosmetic composition having an antioxidant effect and an anti-inflammatory effect, containing 40 to 70% by weight of green tea water-soluble extract, 14 to 40% by weight of jinjin mugwort water extract, 14 to 40% by weight of pine needle water-soluble extract, and 2 to 10% by weight of vitamin C. delete delete delete After extracting hot water from green tea, phosphorus mugwort or pine needles at 80 to 110 ° C. for 1 to 5 hours, the composition is concentrated and dried to a concentration of 10 to 40%, and a dry concentrate and vitamin C having an antioxidant effect and an anti-inflammatory effect. The cosmetic composition according to claim 1 or 5, wherein the composition is used for various creams, lotions and skins. The cosmetic composition according to claim 1 or 5, wherein the composition is used as a shampoo, rinse, cleansing, face wash, soap, treatment, essence. delete

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