KR100844990B1 - A composition comprising novel black ginseng and the extract thereof showing anti-oxidative activity prepared by novel process - Google Patents

Abstract

The present invention relates to a composition containing black ginseng and extracts thereof prepared by a novel manufacturing method for producing black ginseng having an antioxidant activity capable of mass production, which is shorter in time than the black ginseng prepared by the conventional method, and prepared from the same. Black ginseng and its extracts can be used as cosmetic compositions for preventing and improving skin diseases caused by oxidative stress and skin external pharmaceutical compositions for preventing and treating skin diseases caused by oxidative stress.

Black ginseng, mass production, ginseng, antioxidant, skin external pharmaceutical composition, cosmetic composition.

Description

A composition comprising novel black ginseng and the extract etc showing anti-oxidative activity prepared by novel process}

1 is a view showing the Onggi used for steaming black ginseng.

It is an object of the present invention to provide an antioxidant composition comprising black ginseng and its extract as an active ingredient having excellent antioxidant effects as compared to black ginseng prepared by the method of the conventional augmentation.

To capture the $ 3.5 billion global ginseng market, Korean bio venture companies are working hard to develop new global ginseng-related materials worth $ 3.5 billion annually. So far, the only company that has standardized ginseng as a functional food and is making a profit in the global market is “Pharmaton,” a company owned by Boehringer Ingelheim, Switzerland.

Korean red ginseng has a historical record of about 1,000 years ago (there is a record of red ginseng in Goryeo-do, written by the Chinese Song Dynasty). have. In general, the numerical objectives of herbal medicines are known as: 1) reducing side effects such as toxicity, 2) altering herbal properties and enhancing drug efficacy, 3) improving storage and storage, and 4) correcting taste and odor and color. In general, red ginseng undergoes a second ingredient conversion during an appropriate heat treatment process, thereby generating new active ingredients unique to red ginseng or fresh ginseng, and some active ingredients increase in content. As such, new ingredients that do not exist in ginseng are produced according to the processing method of ginseng, and new processed ginseng can be developed that shows better effects on various physiological activities including antioxidant activity than ginseng (Okamura N. et al. , Biol . Pharm. Bull. , 17 (2) , p.270, 1994).

In China, they have developed a method of mass-producing Rg3, a ginseng saponin, which is rarely present in white ginseng and is present in red ginseng, and is used for antioxidant treatment under the trade name 'Shenyi'.

In Korea, the representative product of functional ginseng is 'Seonsam' developed by bio venture company Ginseng Science in 1998.It was developed as Sesamsamjeong in 2001 and recognized in domestic health functional food market. is a receive become the leader of the functional state of ginseng (Keum YS et al., Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng, Cancer Lett.,, 150 (1), pp.41- 48, 2000).

In addition, 'Hwangsam EX' co-developed by Korea Research Institute of Biotechnology and biotechnology company Beatijin, Namseng Farm Co., Ltd., 'Ginseng Red Ginseng' produced by joining Biobio, Green Bio Co., Ltd., and Unigen Co., Ltd. Recently, various types of processed ginseng are being developed, such as Biomax, which was developed jointly with UPI in the US, Six Plus of CJ, and Yellow Ginseng of Ildong Pharm.

As the premium ginseng era is invented, the present inventors use the water and fire together in the processing method of the Chinese herbal medicine, one of the steaming methods. We have developed a standardized method of manufacturing black ginseng which is very short in time compared to the method of preparing black ginseng and can be mass-produced. Also, the black ginseng and its extract prepared by the manufacturing method of the present invention contain a large amount of ginseng saponin. The antioxidant effect was confirmed to complete the present invention.

An object of the present invention to provide an antioxidant composition containing black ginseng and its extract as an active ingredient prepared by a novel manufacturing method excellent in antioxidant effect.

In order to achieve the above object, the present invention is a skin external use for the prevention and treatment of skin diseases caused by oxidative stress containing black ginseng and black ginseng extract obtained through the process of drying, steaming, heating and drying ginseng It provides a pharmaceutical composition and a cosmetic composition for preventing and improving skin diseases caused by oxidative stress.

Hereinafter, the present invention will be described in detail.

Black ginseng and its extract of the present invention can be prepared through the following process. After washing 3 to 7 years old root, preferably 5 years old root ginseng of the present invention, primary drying is carried out at 20 to 60 ° C, preferably 35 to 55 ° C for 12 to 36 hours, preferably 20 to 28 hours. fair; Primary steaming step of steaming for 3 to 9 hours, preferably 5 to 7 hours at a temperature of 60 to 120 ℃, preferably 85 to 105 ℃ except for preheating time in the Onggi; A secondary drying step of drying at a temperature of 40 to 80 ° C., preferably 50 to 70 ° C. for 6 to 18 hours, preferably 9 to 15 hours; A secondary steaming step of steaming at a temperature of 85 to 135 ° C, preferably 95 to 125 ° C for 3 to 9 hours, preferably 5 to 7 hours; The black ginseng of the present invention and its extract may be prepared through a manufacturing method capable of shortening the time through a third drying process of drying for 8 to 16 hours, preferably 10 to 14 hours, and mass production.

The black ginseng manufacturing method is compared with the components of black ginseng prepared by the manufacturing method described in Korean Patent Registration No. 10-0529475 and Korean Patent Registration No. 10-0543862, which are known as the conventional methods for manufacturing black ginseng, and the principle of the existing guts. Ginseng saponin is present at the same or superior level as the black ginseng prepared by using, and the time is shorter than the black ginseng prepared by the conventional method, characterized in that mass production is possible.

The black ginseng or its extract prepared by the above manufacturing method is dried through a drying method common in the art such as shade drying or freeze drying, and then pulverized and powdered into fine particles, preferably a particle size of about 50 μm to 200 μm. It can be prepared in a composition such as pills, capsules, tablets using an excipient or carrier well known in the art through the process.

The black ginseng prepared by the above method may be used as a raw material for various preparations by grinding into fine powder, preferably powder of about 50 to 200 μm, using a herbal medicine grinder.

In addition, the method for obtaining the extract of the black ginseng prepared by the above method will be described in detail. After crushing the black ginseng prepared by the above method, water of about 3 to 7 times, preferably 4 to 6 times the mass of black ginseng is added thereto. After standing at room temperature for 3 to 7 hours, 0.5-2 L of methanol was added and the mixture was extracted under reflux three times for three hours. The extracted solution was cooled to room temperature, filtered, and methanol was removed using a vacuum condenser to obtain an extract of black ginseng prepared by the method of the present invention.

The production method of the present invention can be applied to ginseng leaves as well as ginseng itself. Although ginseng leaves have not been used for medicinal purposes, they have been discarded or used for feed, and some of them have been used as raw materials for cosmetics or foods. However, when ginseng leaf extracts are heated and pressurized in the same way as in the present invention, the medicinal effect is much better. Since it is fortified, it can be used for medicinal purposes as well as the use of ginseng leaves.

The black ginseng prepared by the above method is characterized in that the existing ginseng, white ginseng, or red ginseng does not exist or enhances the content of the active ingredient present in trace amounts.

In addition, black ginseng and black ginseng extract prepared by the production method of the present invention is characterized by showing a strong antioxidant effect compared to red ginseng.

Therefore, the present invention provides a skin external pharmaceutical composition for the prevention and treatment of skin diseases caused by oxidative stress containing black ginseng and black ginseng extract prepared by the above method as an active ingredient.

In another aspect, the present invention provides a cosmetic composition for the prevention and improvement of skin diseases caused by oxidative stress containing black ginseng and black ginseng extract prepared by the manufacturing method as an active ingredient.

Skin diseases caused by the oxidative stress include skin diseases such as skin aging, wrinkles, blemishes, freckles, dermatitis, acne.

The black ginseng and extracts thereof may be used in an amount of 0.001 to 90% by weight, preferably 0.01 to 10% by weight, based on the total weight of the skin external pharmaceutical composition or cosmetic composition.

The skin external pharmaceutical composition containing the black ginseng and extract thereof of the present invention is an external skin agent having an antioxidant effect, such as a cream, gel, patch, spray, ointment, warning agent, lotion, linen, pasta or cataplasma. It may be prepared and used as a pharmaceutical composition in the form of an external preparation, but is not limited thereto.

Preferred dosages of the black ginseng and extracts thereof of the present invention vary depending on the condition and weight of the patient, the severity of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the preferred effect, black ginseng and its extract of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably at 0.001 to 10 mg / kg. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

In addition, the black ginseng of the present invention and extracts thereof may be used in various ways, such as cosmetics and face wash having an antioxidant effect.

Products to which the composition can be added include, for example, cosmetics such as lotion, skin, lotion, nourishing lotion, nourishing cream, massage cream, essence and pack, and cleansing, face wash, soap, treatment, essence, etc. have.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. , Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, dl-pantothenyl alcohol, D-pantothenyl alcohol, pantotenylethyl Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound that can be formulated in cosmetics, but preferably brown algae extract, red algae extract, green algae extract, and the like, and calginin, arginic acid, sodium arginate, purified from these seaweed extracts, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl lauric acid, decyl mysticin, myristic acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, lysinooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoate cetostearyl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, Polyester, Polypropylene, Polystyrene, Polyurethane, Vinyl Resin, Urea Resin, Phenolic Resin, Fluoro Resin, Silicon Resin, Acryl Resin, Melamine Resin, Epoxy Resin, Polycarbonate Resin, Divinylbenzene, Styrene Copolymer And organic pigments such as silk powder, cellulose, CI pigment yellow, and CI pigment orange, and composite pigments of these inorganic pigments and organic pigments.

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5% weight percentage with respect to a total weight, More preferably 0.01-3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Components included in the cosmetic composition of the present invention as an active ingredient may include components commonly used in cosmetic compositions in addition to the black ginseng and extracts thereof, for example, stabilizers, solubilizers, vitamins, pigments and fragrances Conventional adjuvants and carriers are included.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

However, the following Examples and Experimental Examples are only illustrative of the present invention, and the content of the present invention is not limited by the following Experimental Examples.

Comparative Example  1. Red Ginseng Methanol Extract and Butanol  Preparation of Extract

      20 g of red ginseng purchased from Kumsan Cheonnyeon Hongsam Co., Ltd. was pulverized, and 100 ml of water was added and left for 5 hours at room temperature. Then, 1 liter of methanol was added and refluxed three times for 3 hours. The extracted solution was cooled to room temperature and filtered. After removing methanol using a vacuum concentrator, 8.1g of red ginseng methanol extract was obtained. 200 ml of water was added and suspended in 4.05 g of red ginseng methanol extract, and then 200 ml of ether was added to remove the nonpolar material. 200 ml of saturated butanol was added to the remaining aqueous layer to extract saponins present in ginseng, and then butanol was removed using a vacuum condenser to obtain 0.65 g of butanol extract containing a large amount of ginseng saponin. The red ginseng methanol extract and butanol extract were used as comparative samples of the antioxidant experiment.

Example  1. Preparation of Black Ginseng

      After purchasing five-year-old ginseng produced and distributed in Geumsan, the ginseng was put in a screw washer and washed three times in total for 10 minutes. At this time, care was taken not to peel during the cleaning process.

If the washed ginseng steamed without drying, the fresh ginseng is often bursting, so it was first dried at 40 ℃ for 24 hours to prevent this. The ginseng dried at low temperature for 24 hours was placed in Onggi (see FIG. 1) using a steamer, and primary steaming was performed at 95 ° C. for 6 hours except for the preheating time. The preheating time is the time it takes for the steam to come out of the pot and it usually takes about 30 minutes. After keeping the internal temperature of the dryer with a heating wire at 60 ℃, spin the pen and dry the ginseng for 12 hours for 2 hours, then steam it for 2 hours at 115 ℃ for 2 hours, and then dry 3 times in the dryer for 3 hours. Black ginseng of the present invention was prepared.

Example  2. Black Ginseng Methanol Extract and Butanol  Preparation of Extract

      20 g of black ginseng prepared by the method of Example 1 was taken and pulverized, 100 ml of water was added thereto, and the mixture was left at room temperature for 5 hours, followed by 1 liter of methanol, followed by 3 hours of reflux extraction. The extracted solution was cooled to room temperature, filtered, and methanol was removed using a vacuum condenser to obtain 7.4 g of black ginseng methanol extract. 200 g of water was added to 3.2 g of black ginseng extract to suspend and 200 mL of ether was added to remove the nonpolar material. 200 ml of saturated butanol was added to the remaining aqueous layer to extract saponins present in ginseng, and then butanol was removed using a vacuum condenser to obtain 0.7 g of butanol extract containing a large amount of ginseng saponin. The black ginseng methanol extract and butanol extract were used as a sample for component analysis and antioxidant experiment.

Example  3. Analysis of Black Ginseng Saponin Components

In order to analyze the components of ginseng saponin present in the black ginseng extract, 20 mg of the black ginseng butanol extract obtained in Example 2 was dissolved in 1 ml of methanol, and the filtrate was filtered through a 0.45 μm filter. Analyzed. As a result of analyzing the components, Rb1 2.3mg, Rb2 2.1mg, Rc 1.3mg, Rd 1.4mg, Re 0.5g, Rg1 1.2mg, Rk1 3.7mg, Rg5 4.7mg, Rg3 S) was found to be 5.4 mg, Rg 3 (R) 4.5 mg was present. That is, it was confirmed that ginseng saponin existed at an excellent or similar level when compared with the black ginseng prepared using the existing augmentation width principle.

Column: Shiseido C18 column (5㎛, 1504.6mm) Flow rate 1ml / min Detector ELSD-LT detector Solvent A; CH ₃ CN: H ₂ O: 5% CH ₃ COOH = 15: 80: 5, B; CH ₃ CN: H ₂ O = 80:20 0 min (0% B), 0-10 min (30% B), 10-25 min (50% B), 25-40 (100% B), 40- 50 minutes (100% B), 50-55 minutes (0% B), 55-58 minutes (0% B) Temperature 40 degrees

Experimental Example  1. Measurement of Antioxidant Activity

In order to measure the antioxidant activity of black ginseng and its extracts, radical scavenging activity was performed with the red ginseng extract of Comparative Example 1 and the black ginseng extract of Example 2 using TOSC (Total Oxyradical Scavenging Capacity) assay. .

TOSC assays are described in Winston et al., Free Radic. Biol Med ., Feb, 24 (3), pp480-493, 1998) and modified by the same authors (Regoli and Winston, Toxicol Appl Pharmacol ., Apr 15, 156 (2) , pp96-105, 1999). Peroxyl radicals were generated by thermal homolysis of ABAP (2,2'-azobisamidinopropane) at 35 ° C (Winston et al., Free Radic). Biol Med ., Feb, 24 (3), pp 480-493, 1998), hydroxyl radicals are the Fenton reaction using Fe and ascorbate (Fenton reaction; Winston and Cederbaum, Alcholol). Clin Exp Res., 9 (2) , pp95-102, 1985), peroxynitrite was generated through spontaneous decomposition of SIN-1. Each reactive oxygen species generated reacts with α-keto-γ-methiolbutyric acid (KMBA) to generate ethylene gas, which does not show any temperature-dependent difference. It has been reported (Winston et al., Free Radic Biol Med ., 24 (3) , pp 480-493, 1998; Regoli and Winston, Toxicol Appl Pharmacol . , 156 (2) , pp96-105, 1999).

The reaction proceeded by putting the final volume of 1 ml of the reaction solution into a sealed 10 ml container with rubber septum. Ethylene gas generation detection takes a certain volume of air in the head space of the reaction vessel, and is equipped with a gas chromatography equipped with a Poropack N column and a FID (flame ionization detector). Was analyzed.

As a result of the experiment, the area under the kinetic curve (AUC) was obtained by integrating a graph obtained from the experimental measurements, and the TOSC value was calculated through Equation 1 below.

TOSC = 100-(∫SA / ∫CA × 100)

∫SA = range integrated from the curve of the sample response

(integrated area from the curve of the sample reaction)

∫CA = integrated range from curve of control response

(integrated area from the curve of the control reaction)

For a sample having no oxyradical scavenging capacity, ∫SA / ∫CA = 1 and a value of TOSC = 0. Conversely, when ∫SA → 0, the TOSC value approaches 100. The value of TOSC is compared with the value in the control, so it is theoretically unaffected by the sensitivity of the instrument, reagents used, or other reaction conditions.

GC equipped with Poropack N column and FID (flame ionizing detector) for the detection of ethylene gas was placed in an oven at 60 ° C, injector 180 ° C and detector 180. It was fixed at 캜 and helium was injected into the column at a rate of 30 ml / min as the mobile phase. For the determination of the ethylene gas in the vial, 150 μl of air in the vial was taken with a gas-tight syringe using the head-space technique, and then gas-tight. The test was performed by injecting a syringe into an injector.

Antioxidant TOSC value according to concentration Specific TOSC Value (TOSC / mg) Black Ginseng Butanol Extract Concentration (mg / ml) 0.025 0.05 0.1 0.2 476 ± 91 TOSC value 8.10 32.35 45.33 51.80 Black Ginseng Methanol Extract Concentration (mM) 0.025 0.05 0.1 0.2 433 ± 160 TOSC value 28.25 40.92 47.00 64.46 Red Ginseng Butanol Extract Concentration (mM) 0.025 0.05 0.1 0.2 423 ± 122 TOSC value 3.69 31.27 38.87 41.30 Red Ginseng Methanol Extract  Concentration (mM) 0.025 0.05 0.1 0.2 453 ± 88 TOSC value 17.08 34.13 45.98 53.00

Antioxidant TOSC value according to concentration Specific TOSC Value (TOSC / mg) Black Ginseng Butanol Extract Concentration (mg / ml) 0.025 0.05 0.1 0.2 405 ± 39 TOSC value 6.48 22.19 39.07 46.13 Black Ginseng Methanol Extract Concentration (mM) 0.025 0.05 0.1 0.2 355 ± 34 TOSC value 7.65 21.11 34.73 40.70 Red Ginseng Butanol Extract Concentration (mM) 0.025 0.05 0.1 0.2 232 ± 24 TOSC value 4.10 13.33 22.48 31.84 Red Ginseng Methanol Extract Concentration (mM) 0.025 0.05 0.1 0.2 204 ± 9 TOSC value 3.94 10.19 20.00 25.38

As a result of the experiment, red ginseng and black ginseng showed high antioxidant activity against peroxyl radicals and peroxynitrite at 0.2 mg / ml or less. Scavenging of peroxyl radicals was observed to be comparable to red and black ginseng. However, the scavenging ability of peroxynitrite showed about 1.7 times higher activity of black ginseng extract and its methanol extract than red ginseng extract and its methanol extract. These experimental results indicate that the type or ratio of the composition may change depending on the method of aging ginseng, which may cause a difference in the antioxidant effect. (See Table 2 and Table 3)

Experimental Example  2. Acute Toxicity Test

Acute toxicity test was performed using 6-week-old specific pathogen-free (SPF) SD rats from the Korean Center for Experimental Animals. Two animals of each group were orally administered to the black ginseng extract of the present invention at a dose of 1 g / kg. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed, and hematological and hematological examinations were performed.

As a result, no clinical symptoms or dead animals were observed in all animals treated with the test substance, and no toxic changes were observed in weight changes, blood tests, blood biochemical tests, and autopsy findings. As a result, the extract of the present invention did not show a change in toxicity even in rats up to 1 g / kg, respectively, the minimum lethal dose (LD ₅₀ ) was determined to be a safe substance of more than 1 g / kg.

The preparation example of the cosmetic composition comprising the black ginseng or extract thereof of the present invention will be described, but the present invention is not intended to be limited thereto, but is intended to be described in detail only.

Formulation example  1. Preparation of Hydrophilic Ointment

Raw material weight%   Black Ginseng or Black Ginseng Extract 9.00   White vaseline 36.00   Stearyl alcohol 31.00   Ethyl (or methyl) p-oxybenzoate a very small amount   Propylene glycol 19.00   Sodium lauryl sulfate 2.40   Propyl p-oxybenzoate a very small amount system 100.00

Formulation example  2. Flexible Cosmetics skin Manufacturing

Raw material weight%   Black Ginseng or Black Ginseng Extract 2.0 1,3-butylene glycol 2.00 glycerin 3.00 Oleyl alcohol 2.00 Polysorbate 20 1.00 ethanol 6.00 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100.00

Formulation example  3. Milk croissant  Produce

Raw material weight%   Black Ginseng or Black Ginseng Extract 5.0 1,3-butylene glycol 4.00 glycerin 3.00 Oleyl alcohol 0.05 Polysorbate 20 1.00 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100.00

Formulation example  4. Manufacturing of Nutritional Cream

Raw material weight%   Black Ginseng or Black Ginseng Extract 2.0 Vaseline 5.00 Liquid paraffin 30.00 Beeswax 3.00 Polysorbate 60 1.00 Sorbitan sesquioleate 1.00 1,3-butylene glycol 5.00 glycerin 5.00 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100.00

Formulation example  5. Massage  Manufacture of cream

Raw material weight%   Black Ginseng or Black Ginseng Extract 7.0 glycerin 5.00 Triethanolamine 0.50 Tocopheryl acetate 1.00 Liquid paraffin 5.00 Squalane 5.00 Macadamia Nut Oil 15.00 Polysorbate 60 1.00 Sorbitan sesquioleate 1.00 Carboxy vinyl polymer 0.20 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100.00

As described above, the present invention has a shorter time compared to the black ginseng prepared by the existing augmentation method, and is capable of mass production. Provided is a skin composition for the prevention and treatment of skin diseases caused by oxidative stress, and a cosmetic composition for the prevention and improvement of skin diseases caused by oxidative stress.

Claims (2)

delete After washing 4 to 6 years old ginseng, the first drying process to dry for 20 to 28 hours at a temperature of 35 to 55 ℃: primary steaming process steamed for 5 to 7 hours at a temperature of 85 to 105 ℃ in the Onggi: Second drying process drying for 9 to 15 hours at a temperature of 50 to 70 ℃ Secondary steaming process to steam for 5 to 7 hours at a temperature of 95 to 125 ℃ manufacturing comprising a third drying process step drying for 12 hours Cosmetic composition for the prevention and improvement of skin aging, wrinkles, blemishes, freckles, dermatitis or acne skin disease due to oxidative stress containing black ginseng extract obtained by the method.

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