KR20160147136A - A composition for skin whitening or regeneration comprising Melandrium firmum extract - Google Patents

Abstract

The present invention relates to a composition containing a Melandrium firmum extract as an active ingredient for skin whitening or regeneration and a method for using the same. Particularly, the composition containing the Melandrium firmum extract of the present invention shows excellent skin whitening effects through suppression of melanogenesis and pigmentation and also shows excellent skin regeneration through activities of removing free radicals and promoting cell proliferation. Also, the composition does not cause side effects even when being applied to a human body, thereby having high usability in a cosmetic composition, a pharmaceutical composition, a functional health food composition, or a quasi-drug composition.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for skin whitening or regeneration,

The present invention relates to a skin whitening or regenerating composition containing a quail extract and a method using the same.

The skin acts as a barrier against the external environment and protects the living environment from the surrounding environment by protecting against the entry of foreign pollutants or microorganisms including chemicals and ultraviolet rays.

The skin color of a person is determined by the amount of melanin, carotene, and hemoglobin, and melanin is the most important factor. The melanin pigment, a phenolic high molecular substance with a complex form of black pigment and protein, acts as a UV barrier. Therefore, a person who lacks the melanin pigment is very sensitive to sunlight, so that it is prone to burn, and the probability of skin cancer is increased. Ultraviolet rays and carcinogens of short wavelengths form harmful free radicals in the skin. Melanin plays a useful role in protecting proteins and genes by removing such free radicals. Thus, a high amount of melanin means that it has an effective countermeasure to protect the skin from physical or chemical toxicants.

Melanin is produced by a complex process from tyrosine by the action of tyrosinase present in the pigment cells. The resulting melanin is transferred to the skin cells, and melanin is lost with epidermal detachment, and the circulating action appears to disappear. That is, when the skin is exposed to ultraviolet rays, tyrosinase is activated. The tyrosinase activates tyrosine present in the skin tissue to induce an oxidation process of producing dopa (DOPA) and dopaquinone, Melanin is synthesized from melanomas in melanocytes. The synthesized melanin is transferred to keratinocytes, the keratinocytes of the skin, and reaches the skin surface by the keratinization process to protect the skin from ultraviolet rays. However, when melanin is overly synthesized locally, or when the skin physiological function is deteriorated due to skin lesions and aging, melanin is deposited on the skin surface and causes various pigmentation such as spots and freckles.

As described above, the cause and mechanism of skin pigmentation (blackening) are revealed, and prevention of melanin pigment deposition in skin has been mainly studied in the following four perspectives:

1) development of an antagonist against tyrosinase synthesis inhibitor or tyrosinase substrate to regulate tyrosinase activity, the main enzyme of melanin synthesis;

2) to develop a substance that shows toxicity to melanocytes in order to lower the function of melanocyte site, which is the place of melanin biosynthesis in animals;

3) development of dopa reducing materials to prevent the oxidation of dopamine (DOPA), an intermediate metabolite of the melanin synthesis pathway;

4) a first enzyme tyrosinase which is a melanin producing mechanism, a second enzyme dopachrome tautomerase which catalyzes the conversion of DOPA chrome to DHICA (5-6, dihydroxyindole-2-carboxylic acid) DOPA chrome tautomerase) and the activity of a third enzyme that catalyzes the conversion of DHICA to indole-5,6-quinone-2-carboxylic acid Method development.

On the other hand, representative materials used for preventing or improving the deposition of skin pigment include kojic acid, arbutin, hydroquinone, hydroquinone, which are substances which inhibit tyrosinase enzyme activity, Vitamin C (L-ascorbic acid) and derivatives thereof or various plant extracts. However, the use of these substances is limited due to the decomposition and coloring in the prescription system, the generation of odor, the uncertainty of the efficacy or effectiveness at the living body level, or the safety problem.

Kojisan inhibits enzymatic activity by adsorbing copper ions present in the active site of tyrosinase, causing problems such as unstability and skin side effects when added to cosmetics, and recent animal tests have shown that it induces liver cancer, Was discontinued. Vitamin C and its derivatives are difficult to use as a raw material for cosmetics because of its good oxidative instability. Hydroquinone has excellent whitening effect on the skin, but it has allergen-inducing properties, toxicity to melanin-producing cells, Is high. In addition, recently, it is prescribed as a carcinogenic substance, and only limited concentration is allowed in each country. Arbutine is a derivative in which glucopyranoside is bound to hydroquinone. It has little side effects when hydroquinone is used, has no toxicity to the human body, inhibits the synthesis of melanin pigment, and increases melanin pigmentation However, it is also disadvantageous in that it is partially decomposed by skin enzymes. Therefore, it is urgent to develop a safe alternative whitening agent which is excellent in efficacy even in a small amount and has few side effects.

Under these circumstances, the present inventors have made intensive efforts to develop a substance having the effect of improving or improving the whitening or regeneration of skin, and as a result, it has been found that the quail extract effectively inhibits melanin synthesis and also inhibits free radical scavenging, And the cosmetic composition for skin whitening or regeneration using the same was completed.

It is an object of the present invention to provide a method for the production of melanin firmum ) as an active ingredient.

It is another object of the present invention to provide a skin whitening or regenerating cosmetic composition comprising an extract of royal flush as an active ingredient.

It is still another object of the present invention to provide a skin whitening or regenerating pharmaceutical composition containing an ascorbic acid extract as an active ingredient.

It is another object of the present invention to provide a skin whitening or regenerating health functional food composition comprising an extract of King Fahrenheit as an active ingredient.

It is another object of the present invention to provide a skin whitening or quasi-quasi-composition for regeneration, which comprises a quail extract as an active ingredient.

Yet another object of the present invention is to provide a skin whitening or regenerating method comprising the step of administering to a subject a composition containing an ascorbic acid extract as an active ingredient.

As one aspect for solving the above object, the present invention provides a skin whitening or regenerating composition containing an extract of King Fungus as an active ingredient.

The composition may be a cosmetic, a pharmaceutical, a health functional food or a quasi-drug composition.

Wangbul fashion (Melandrium firmum) is a biennial herbaceous jangguchae belonging to the Dianthus (Caryophyllacae) (Melandrium firmum and Melandrium apricum , and it has been known to be used for treatment of water menstruation, milk sickness , and gingival bleeding due to its efficacy, such as blood clotting, However, the effect of skin whitening or regeneration is not known, and research on human skin has not been performed sufficiently.

The term "extract " of the present invention means a substance obtained by dissolving a substance in a solvent, and specifically refers to a substance obtained by extracting a dilute solution or a concentrated solution of the extract, Extracts of all the formulations which can be formed using the extract itself and the extract such as the dried product, the adjusted product or the purified product of the above extract, or a mixture thereof.

The extract of the present invention can be extracted from natural, hybrid, or variant plants of plants, and can also be extracted from plant tissue cultures.

There is no particular limitation on the method for extracting the quadrillion fimbriae of the present invention, and extraction can be carried out according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.

There is no particular limitation on the type of solvent used for extracting the above-described quadriceps, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C ₁ to C ₄ lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof. Specifically, water, a lower alcohol, 1,3-butylene glycol and ethyl acetate may be used alone or in combination of two or more. More specifically, in the present invention, water (or distilled water) may be used as a solvent for extracting the quail. The solvent extract may be prepared by extracting at least one round-trip epidemic using the solvent, and the dry extract obtained by vacuum distillation or spray-drying the solvent extract may be prepared. In one embodiment of the present invention, water was used as an extraction solvent to prepare a quail extract (Example 1).

The extract extracted with hot water in the present invention may be filtered to remove suspended solid particles, for example, by filtration using nylon or the like, or by filtration using a freeze filtration method or the like, or the extract may be used as it is or may be freeze-dried, It may be dried and used.

The term "whitening" of the present invention means to whiten skin, and means to alleviate or improve various pigmentation including stains and freckles due to excessive synthesis of melanin or melanin deposition.

In one embodiment of the present invention, the effect of inhibiting melanin synthesis at the cellular level was examined using mouse melanoma cells to confirm skin whitening effect of the quail extract of the present invention. As a result, (Table 1), which is superior to melatonin (Arbutin). In addition, sensory evaluation was carried out to examine the improvement of pigmentation by applying a skin external preparation or essence containing an extract of royal flush to 20 men and women, and it was found that the royal flush extract has excellent effect for improving pigmentation such as spots or freckles (Table 6).

The term "regeneration" of the present invention means a restoration process of skin tissue against skin (cell) damage caused by external and internal causes of the skin. Damage due to external causes may be caused by ultraviolet rays, external contaminants, wounds, trauma, etc. Damage due to internal causes may be caused by stress or the like.

In one embodiment of the present invention, the free radical scavenging ability of DPPH method was analyzed to confirm the skin regeneration effect of the quail extract, and human fibroblasts were cultured and analyzed for cell proliferation promoting activity by MTT reduction method. As a result, it was confirmed that the quail extract showed excellent free radical scavenging ability as compared with ascorbic acid used as a free radical scavenger (Table 2), and showed excellent cell proliferation promoting activity in a concentration-dependent manner (Table 3).

In another aspect of the present invention, the present invention provides a cosmetic composition for skin whitening or regenerating, which contains an extract of royal flush as an active ingredient.

The cosmetic composition of the present invention may contain 0.0001 to 50% by weight of a quintile extract, specifically 0.01 to 10% by weight, based on the weight of the total composition. There is an advantage of exhibiting excellent skin whitening or regenerating effect within the above range, and there is an advantage that the formulation of the composition is stabilized.

The cosmetic composition of the present invention can be used as a cosmetic composition in the form of a solution, an ointment for external use, a cream, a foam, a nutritional lotion, a flexible lotion, a pack, a flexible water, a milky lotion, a makeup base, But are not limited to, emulsions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays It is not.

The cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a general skin cosmetic composition. Examples of the cosmetic composition include ordinary ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, , A chelating agent, a coloring agent, a preservative, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the formulations of the cosmetic composition.

When the formulations of the present invention are ointments, pastes, creams or gels, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder and the like may be used as a carrier component. In particular, But are not limited to, propellants such as rocaborn, propane / butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like can be used, and particularly fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a soap, use is made of an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, glycerol, But is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is an interfacial active agent-containing cleansing, it is preferable to use, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester, but are not limited thereto. These may be used alone or in combination of two or more.

In another aspect of the present invention, the present invention provides a skin whitening or regenerating pharmaceutical composition containing an extract of King Fungus as an active ingredient.

The pharmaceutical composition of the present invention may contain 0.0001 to 50% by weight, preferably 0.01 to 10% by weight, of the kingfisher fashionable extract based on the weight of the total composition. There is an advantage of exhibiting excellent skin whitening or regenerating effect within the above range, and there is an advantage that the formulation of the composition is stabilized.

The pharmaceutical composition may be any one selected from the group consisting of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterilized aqueous solutions, nonaqueous solvents, suspensions, emulsions, And may be oral or parenteral formulations of various forms.

In the case of formulation, it can be prepared by using diluents or excipients such as fillers, extenders, binders, humectants, disintegrants, surfactants and the like which are usually used.

When the formulation of the present invention is a solid preparation for oral administration, it may contain tablets, pills, powders, granules, capsules and the like, and the solid preparation may contain at least one excipient such as starch, Calcium, sucrose or lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may be used.

When the formulation of the present invention is a liquid preparation for oral administration, it may contain a suspension, a solution, an emulsion, a syrup, etc. In addition to water and liquid paraffin which are simple diluents commonly used, various excipients such as wetting agents , Sweeteners, fragrances, preservatives, and the like.

When the formulation of the present invention is a preparation for parenteral administration, it may contain a sterilized aqueous solution, a non-aqueous solvent, a suspension, an emulsion, a lyophilized preparation, and a suppository. Examples of the non-aqueous and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like. Examples of the suppository include witepsol, , Macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like can be used.

As another embodiment for solving the above object, there is provided a skin whitening or regenerating health functional food composition comprising an extract of King Fahrenheit as an active ingredient.

The health functional food composition may be in the form of any one of meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, gum, ice cream, soup, beverage, tea, functional water, Lt; / RTI >

It is also possible to use a variety of nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and heavies (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, The composition may contain a colloidal thickener, a pH adjusting agent, a stabilizer, a preservative, a glycerin, an alcohol, a carbonating agent used in a carbonated beverage, and the like, as well as flesh for the production of natural fruit juice and fruit juice drinks and vegetable drinks . These may be used alone or in combination of two or more.

The health functional food composition may further include food additives and the suitability of the food additives as "food additives" is to be determined by the General Regulations of the Food Additives Revision approved by the Food and Drug Administration, Shall be determined according to the relevant standards and standards.

Examples of the products listed in the above-mentioned "food additives" include natural products such as ketones, chemical products such as glycine, potassium citrate, nicotinic acid and cinnamic acid, coloring matter, licorice extract, crystalline cellulosic, high- , A sodium L-glutamate preparation, a noodle-added alkaline agent, a preservative preparation, a tar coloring agent, and the like.

At this time, the extract according to the present invention, which is added to foods containing beverages in the process of manufacturing a health functional food, can be appropriately added to or subtracted from the extract according to necessity. Specifically, 0.01 to 10% can do.

In another aspect of the present invention, there is provided a whitening or regenerating quasi-product composition comprising an extract of King Fungus as an active ingredient.

The quasi-drug composition of the present invention can be used as a body cleanser, a disinfectant cleaner, a detergent, a kitchen detergent, a cleaning detergent, a toothpaste, a gauze agent, a wet tissue, a detergent, a soap, a hand wash, a hair cleaner, a hair softener, a humidifier filler, But the present invention is not limited thereto.

In another aspect of the present invention, there is provided a skin whitening or regenerating method comprising the step of administering to a subject a composition containing an ascorbic acid extract as an active ingredient.

The term "individual" of the present invention means all animals, including humans, that require the effect of skin whitening or regeneration, and the term " administering "Quot; means introducing the pharmaceutical composition of the present invention. The administration route of the composition can be administered through various routes of oral or parenteral administration, as long as it can reach the target tissue. Specifically, they may be administered by a transdermal administration method such as, but not limited to, topical application.

The skin whitening or regenerating method of the present invention may comprise administering a pharmaceutical effective amount of a pharmaceutical composition comprising a quail extract, or a pharmaceutically acceptable salt thereof. Suitable total daily doses may be determined by the practitioner within the scope of sound medical judgment, and for purposes of the present invention, the specific therapeutically effective amount for a particular patient will depend upon the type and extent of the desired response, Including the age, weight, general health status, sex and diet of the patient, the time of administration, the route of administration and the fraction of the composition, the duration of treatment, the drugs used or co-used with the specific composition It is desirable to apply differently depending on the factor and similar factors well known in the medical field.

The composition comprising the quail extract of the present invention has a skin whitening or regenerating effect. Specifically, the composition of the present invention is excellent in inhibiting melanin synthesis, inhibiting pigment deposition, eliminating free radicals, and promoting cell proliferation.

Therefore, the composition of the present invention, which is a material derived from a natural product, does not cause side effects even when it is applied to human body, and thus can be effectively used as a cosmetic composition, a pharmaceutical composition, a health functional food composition or a quasi-drug composition.

Hereinafter, the constitution and effects of the present invention will be described in more detail through examples. These embodiments are only for illustrating the present invention, and the scope of the present invention is not limited by these embodiments.

Example  One: A fad  Preparation of extract

1 L of distilled water was added to 50 g of the purchased royal flue, which was boiled for 4 hours, filtered, and dried to obtain 8.5 g of a powder.

Example  2: Melanin production inhibition test

In order to confirm the whitening effect of the royal flush extract at the cell level, the obtained royal flush extract was added to the melanoma cell (B-16) culture solution of the mouse to confirm the melanin production inhibitory effect.

The final concentration of 0.1 ㎍ / ㎖, 1 ㎍ / ㎖ or 10 ㎍ / ㎖ of the raffinate extract was added to the culture medium of B-16 melanoma cells and cultured for 3 days. The cells were then trypsinized, detached from the culture vessel, centrifuged, and extracted with melanin. One milliliter of sodium hydroxide solution (1N) was added to the extracted melanin. Melanin was boiled for 10 minutes, and the absorbance of the melt was measured at 400 nm using a spectrophotometer. The amount of melanin produced was measured by the method of measuring absorbance per unit cell number (10 ⁶ cells). At this time, two control groups were used: a negative control group without the royal flush extract and a positive control group supplemented with 200 mg / ml of arbutin for the negative control group.

The amount of melanin production in the cells supplemented with the quail extract was calculated as the inhibition rate (%) for the negative control, and the results obtained by repeating the three experiments are summarized in Table 1 below.

sample Melanin production (ng / ml) Inhibition rate (%) Negative control group (no addition) 0.060 0.002 - Positive control group
(Negative control + arbutin (200 mg / ml)) 0.034 ± 0.001 44% (0.1 mu g / ml) 0.031 ± 0.001 48% (1 쨉 g / ml) 0.020 0.001 67% (10 < RTI ID = 0.0 > pg / ml) 0.018 0.001 70%

As can be seen from the results of Table 1 above, when treated with the royal flush extract, at least 48% of the melanin formation inhibitory effect was exhibited as compared with the negative control without the royal flush extract, and the royal flush extract was generally a whitening substance It was confirmed that melanin synthesis inhibition effect was significantly superior to that of known arbutin-based control group.

Example  3: Analysis of skin regeneration effect

Example  3-1: Free radical Scatters  analysis

In order to examine the effect of the quail extract on the production of free radicals which cause skin damage (skin cell regeneration), the maximum absorption at 517 nm in the presence of radicals is observed, and when the radicals are eliminated, Free radical scavenging activity was measured by the relatively stable free radical 1,1-diphenyl-2-picryl hydrazine (DPPH) method, which has the property of losing its activity. DPPH (purchased from Sigma) was dissolved in methyl alcohol at a concentration of 0.15 mM.

First, 100 μl of each of the wells of the 96-well plate was added with 100 μl of the DPPH solution, and then left at room temperature for 30 minutes. The absorbance was then measured at 517 nm using a Micro plate reader (BiotTek EL-340). At this time, control group 1, in which ascorbic acid, which is known as a free radical scavenger, was added and control group 2 in which methyl alcohol having free radical scavenging ability was added in the absence of the royal flush extract.

The results are shown in Table 2 as IC ₅₀ (Inhibitory Concentration 50), which is the concentration of the quail extract when the absorbance of the test group treated with the sample is half the absorbance of the control group.

division A fad Control 1
(Ascorbic acid) Control group 2
(Methyl alcohol) IC ₅₀ 0.0008% 0.005% 0.0025%

As can be seen from the results of Table 2 above, the treatment with the quail extract showed a remarkably superior free radical scavenging ability compared with the control group 1 treated with ascorbic acid. In addition, compared with the control group 2 treated with methyl alcohol, Which shows the effect of erasing.

Example  3-2: Cell proliferation Bow performance  Measure

In order to confirm the effect of the quail extract on the activation of skin cell proliferation, the cell proliferation activity of human fibroblast was measured.

After adding the royal flush extract of various concentrations (0.01 ㎍ / ㎖, 0.1 ㎍ / ㎖, 1 ㎍ / ㎖, 10 ㎍ / ㎖) to the culture medium, human fibroblasts were cultured to perform MTT reduction, formazan were extracted with isopropyl alcohol and the absorbance at 570 nm was measured. At this time, two control groups were used: a negative control using FBS (fetal bovine serum) and a culture solution without the quail extract, and a positive control using culture with 10% FBS.

The result of the measurement was repeated five times, and the results were calculated by numerical values using the following Equation 1, and are summarized in Table 3 below.

[Equation 1]

Cell proliferation ability (%) = Angle In the sample group  Absorbance / absorbance in the control group

sample Concentration (/ / ml) Cell proliferation activity (%)

Kingfisher fashionable extract 0 100.0 0.01 113.1 0.1 118.6 One 122.6 10 166.0 The positive control (10% FBS) - 131.2

As shown in the results of Table 3 above, the cell proliferation activity was 113.1% at the minimum experimental treatment concentration (0.01 μg / ml) in the case of treatment with the quail extract, (100%) compared to the control group.

Accordingly, it has been confirmed that the extract of the present invention can be effectively used as a cosmetic composition for regenerating damaged skin, for example, a skin external preparation, a pharmaceutical composition or a health functional food composition, because of its excellent cell activity effect through cell proliferation Respectively.

Manufacturing example  1 and Comparative Example  1: Preparation of external preparation for skin

Skin whitening or regenerating dermatological external preparations containing, as an active ingredient, a quadriceps fashion extract was prepared according to a conventional method (Production Example 1). In addition, the external preparation for skin prepared from the components other than the active ingredient was prepared with the composition of Comparative Example 1.

Composition (% by weight) Production Example 1 Comparative Example 1 Melandrium firmum 0.2 - Diethyl sebacate 8.0 8.0 Nonsense 5.0 5.0 Polyoxyethylene oleyl ether phosphate 6.0 6.0 Sodium benzoate 1.0 1.0 vaseline ~ 100 ~ 100

Manufacturing example  2 and Comparative Example  2: Preparation of essence

Skin whitening or regenerating essence containing an extract of King Fungus as an active ingredient was prepared according to a conventional method (Production Example 2). In addition, an essence prepared from the other ingredients except for the active ingredient was prepared with the composition of Comparative Example 2.

Composition (% by weight) Production Example 2 Comparative Example 2 Melandrium firmum 0.5 - glycerin 10.0 10.0 Sodium hyaluronate aqueous solution (1%) 5.0 5.0 Polyoxyethylene hardened castor oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 Spices 0.05 0.05 Purified water ~ 100 ~ 100

Example  5: Sensory experiment

Example  5-1: Pigmentation inhibition effect analysis

In order to confirm the effects of the external preparation for skin and the essence produced according to the compositions of Preparation Examples 1 and 2 against skin pigmentation inhibition, comparative sensory tests were conducted.

Twenty healthy men and women were selected, and aluminum foil with 6 rows of 7 ㎜ holes was attached to the lower part of both arms, and light intensity of 60 mJ / ㎠ was measured using ORIEL solar simulator 1000W at a distance of 10 ㎝ from the arm Respectively. Before irradiation, the irradiated area was washed with 70% ethanol aqueous solution and the experiment was conducted. The external preparation for skin and the essence prepared in accordance with Preparation Examples 1 and 2 and Comparative Examples 1 and 2 were applied to each row three days before irradiation to three weeks after irradiation, twice per day. The degree of discoloration was visually determined, and the degree of suppression of pigmentation by the above-mentioned production example was evaluated by two steps of effectiveness or no effect. The results are shown in Table 6 below.

division Effective (persons) No effect (persons) Production Example 1 17 3 Production Example 2 15 5

As shown in the results of Table 6, the external preparation for skin and essence containing the extract of the present invention showed a whitening effect in comparison with the external preparation for skin and essence of Comparative Examples which did not contain the extract of royal flush, .

Accordingly, it has been confirmed that the extract of the present invention or the composition containing the same can be effectively used as a skin external preparation or cosmetic for improvement of spots or freckles or skin whitening, and further confirmed to be safe for skin.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

Claims (10)

Melandrium firmum ) as an active ingredient.
The composition of claim 1, wherein the composition is a cosmetic, pharmaceutical, health functional food or quasi-drug composition.
2. The composition of claim 1, wherein said composition comprises from 0.01% to 10% by weight of a flavor-rich extract of the total composition.
The composition of claim 1, wherein the skin whitening is due to melanin synthesis inhibitory activity.
The composition of claim 1, wherein the skin regeneration is by free radical scavenging ability.
The composition according to claim 1, wherein the skin regeneration is by cell proliferation promoting activity.
The cosmetic composition according to claim 2, wherein the cosmetic composition is at least one selected from the group consisting of a solution, an ointment for external use, a cream, a foam, a nutritional lotion, a flexible lotion, a pack, a flexible water, a latex, a makeup base, , A formulation selected from the group consisting of suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays Composition.
The health functional food composition according to claim 2, wherein the health functional food composition is at least one selected from the group consisting of meat, sausage, bread, chocolates, candies, snacks, confectionery, pizza, ramen, gum, ice cream, soup, ≪ / RTI > or a combination thereof.
The composition according to claim 2, wherein the quasi-drug composition has a form selected from the group consisting of a body cleanser, a wet tissue, a soap, a hand wash, a humidifier filler, and an ointment agent.
A method for skin whitening or regenerating comprising the step of administering to a subject other than humans a composition comprising an efflorescent foliar extract as an active ingredient.