KR20180014356A - Composition for alleviating skin irritation and skin inflammation induced by particulate matter comprising extracts mixture of Ocimum sanctum, Mentha haplocalyx, and Azadirachta indica - Google Patents

Abstract

The present invention relates to a skin external preparation composition for alleviating skin inflammation comprising a natural extract mixture as an active ingredient. According to the present invention, a skin external preparation composition comprising an extract mixture of Ocimum sanctum, Mentha haplocalyx and Azadirachta indica as an active ingredient inhibits expression of inflammation inducing cytokines (IL-6, IL-8) induced by fine dust and alleviates irritation and skin inflammation induced by fine dust, thereby being effectively applied for preventing and alleviating skin troubles, alleviating flush and preventing and alleviating dermatitis.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for alleviating skin irritation and skin irritation caused by fine dusts containing an extract of holli basil, peppermint, mint tree as an active ingredient, and a skin inflammation induced skin inflammation induced by particulate matter comprising extracts mixture of Ocimum sanctum, Mentha haplocalyx, and Azadirachta indica}

The present invention relates to a composition for external application for skin containing a natural mixture having an activity of alleviating skin irritation and alleviating skin irritation, and more particularly to a composition for external application for skin, which comprises an extract mixture of holly basil, peppermint, Skin external composition for relaxation.

One of the most important functions of the skin is its protective function as a barrier. Skin, which is directly exposed to the external environment, is the most important primary line of defense against body fluids and protecting the body from harmful environments. It also acts as a barrier against toxic substances, microorganisms, physical stimuli or ultraviolet rays. However, when the skin barrier function receives various stimuli both inside and outside the skin, the skin barrier is damaged and the skin becomes dry or inflammation reaction occurs to the skin. In addition, failure to effectively control the initial inflammatory response at this time can lead to severe dermatitis.

However, due to the effects of population growth, urbanization, industrialization, and automobile growth, air pollution is intensifying and the types of pollutants are becoming diverse. Especially, due to the local factors of Korea, it is faced with damages such as dust and fine dust due to the movement of air pollutants emitted from China, and its seriousness is also increasing. Particulate matter is classified into fine dust (PM10) with a diameter of 10 ㎛ or less and ultra fine dust (PM2.5) with a diameter of 2.5 ㎛ or less depending on the particle size. Korea's Ministry of Environment regulates PM10 as an air pollutant. Air pollutants, such as fine dust, are known to cause respiratory and heart diseases, and their effects on the skin, which occupies a large area in the body, are also increasingly being gained. Especially, it is reported that fine dust among air pollutants damages epidermal barrier function and worsens atopic dermatitis. In addition, sensitive skin responds to external stimuli or environmental changes more sensitively than normal skin, resulting in irritation or dermatitis symptoms. Those who have sensitive skin have more than three times more irritation than those who have normal skin. Therefore, pollutants such as fine dusts are likely to cause skin problems, especially for people with sensitive skin. This also affects skin aging, but recent epidemiological studies have shown that skin exposed to fine dust increases wrinkles and pigmentation spots and is directly related to aging. Fine dusts produce active oxygen, which can inhibit collagen synthesis and activate degradation. Polycyclic aromatic hydrocarbons (PAH) adsorbed on the fine dust surface activate the metabolism of the living body through the AhR to increase collagen degradation. do. Therefore, a new treatment method should be sought by studying the ways of reducing direct contact between fine dust and skin and studying molecular mechanisms.

The inflammatory response in the skin begins as an action to defend skin damage caused by physical stimuli, chemicals, and bacteria, and involves a variety of immune cells and inflammation-inducing cytokines. IL-6 (interleukin-6), IL-8 (interleukin-8) and IL-1β (interleukin-β) are typical inflammation-inducing cytokines. IL-1β is a typical cytokine that is produced by activated mononuclear cells, epithelial cells, and vascular endothelial cells and mediates the inflammatory response. IL-6 stimulates the immune response by secretion by T cells and macrophages, It is a cytokine that regulates. Activated macrophages also activate inflammatory processes by overexpressing nitric oxide (NO) or prostagladin E2 (PGE2) as well as inflammation-inducing cytokines. It has been reported that accelerated and anti-inflammatory microparticles increase the expression of these inflammatory cytokines in human keratinocytes.

Conventionally, various attempts have been made to develop cosmetics capable of imparting anti-inflammatory function to skin using natural extracts. As a typical example thereof, Korean Patent Publication No. 2007-0011939 proposes a cosmetic composition containing camphor flower extract Korean Patent Publication No. 2010-0086698 proposes a cosmetic composition for skin soothing and irritation mitigation comprising an extract of mung bean fermentation-enzyme, Korean Patent Laid-Open Publication No. 2013-0016930 discloses a cosmetic composition for sily, lemon balm, mugwort and onion extract As an active ingredient, to cosmetic compositions for alleviating atopic skin and skin irritation. Korean Patent Laid-Open Publication No. 2015-0078948 proposes a cosmetic composition for improving skin irritation or alleviating skin irritation, which comprises a mixed extract of mistletoe, pine bark and gossypol as an active ingredient. In Korean Patent Publication No. 2009-0091547, A cosmetic composition for antiinflammation and skin irritation mitigation containing a mixed extract of casualties is proposed. However, all of the above-mentioned cosmetic compositions are effective in synergistically effectively inhibiting the production of inflammation-inducing cytokines by using an extract according to a specific combination of natural products such as the present invention, thereby providing a cosmetic composition having a skin irritation- No suggestion or suggestion at all.

As a result of efforts to overcome the problems of the prior arts, the inventors of the present invention have found that a substance which recognizes that inflammatory cytokines increased by various stimuli, especially fine dust stimuli, are related to skin irritation and skin irritation, To investigate, plant extracts inhibiting the expression of inflammatory cytokines were screened and screened to compare the inhibition of IL-6 and IL-8 expression, which are inflammation-inducing cytokines, when holly basil, peppermint, Thereby confirming the efficacy of alleviating skin irritation and skin inflammation, thereby completing the present invention.

KR 10-2007-0011939 A KR 10-2010-0086698 A KR 10-2013-0016930 A KR 10-2015-0078948 A KR 10-2009-0091547 A

The present inventors investigated the degree of suppression of inflammation-induced cytokines in a wide range of extracts obtained from various natural products and combinations thereof. As a result, it was confirmed that the combination of specific natural products showed a remarkable cumulative synergistic effect, The present invention provides a composition for external application for skin irritation which has an effect of alleviating skin irritation and skin inflammation by inhibiting IL-6 and IL-8 expression, which are inflammation-inducing cytokines increased by dust stimulation.

Accordingly, a main object of the present invention is to provide a composition for external application for skin having a skin irritation alleviation and skin irritation alleviating effect by synergistically suppressing the production of inflammation-inducing cytokines by using a complex extract according to a specific combination of natural products will be.

The second object of the present invention is to provide a cosmetic composition which can prevent skin irritation and alleviate skin irritation by applying the base formulation of other functional cosmetic composition to prevent and improve skin irritation, improve redness, prevent and improve dermatitis .

According to a preferred embodiment of the present invention to achieve the object of the present invention as described above, it is possible to provide a skin external stimuli for skin irritation and skin inflammation relief by fine dust containing an active ingredient of holli basil, A composition is provided.

Holic basil ( Ocimum sanctum) is a perennial plant belonging to the family Lamiaceae. Tropical Asiatic India and Asia are native to the Pacific Island, which is about 40m high. The scent of basil cleanses the head and eliminates the headache. Drinking tea is beneficial not only for nervous irritation and headache but also for stomatitis. It is also good for promotion of digestion and promotion of food. It stimulates the movement of the brain and improves the symptoms of headache. In addition, skin moisturizing effect (Korean Patent No. 10-1599256) and enhancing effect of oxidative proteolytic enzyme activity (Korean Patent Laid-Open Publication No. 2015-0119336) of cosmetic composition including basil extract are known.

Mintha ( Mentha haplocalyx ) is a perennial herbaceous perennial plant with a monocotyledonous plant. In summer, in the fall, the upper petiole of the stem has a thin purple flower with a spike shape on its armpits. The efficacy is used for fever, headache and sweating symptoms caused by external cold, and it is used for congestion of eyes, sore throat, tonsillitis, early itching skin, liver disease, abdominal pain, diarrhea and vomiting. Pharmacological actions have been reported such as fever, inflammation, dryness, promotion of bile secretion and gastrointestinal smooth muscle suppression, increase of mucus secretion of the respiratory tract, central nervous excitement, and uterine contraction. The main ingredient of peppermint is menthol, which is used medicinally in the form of a spreading agent, an analgesic, a stimulant, a stimulant, a rinse aid, an insect repellent, or a toothpaste, jam, candy, cosmetics, (Korean Patent Laid-Open Publication No. 2013-0016929) and a whitening effect of a cosmetic composition containing an acid mint extract as an active ingredient (Korean Patent Laid-open Publication No. 2009-0018459) are known as cosmetic compositions containing peppermint extracts .

Azadirachta indica is a semi-evergreen tree belonging to the myrtle family. It grows in subtropical and tropical areas and is a native tree in India and neighboring countries. It has been used as food, medicines and insecticides. Its components are limonoids, Azadirachtin, salannin, meliantriol, and nimbin. In Korea, there are many kinds of marbled beech trees. The stem was peeled off and dried, and the dried fruit was used as a treatment for parasites, colic, and malaria. The anti-acne effect of a cosmetic composition including nuts extract (Korean Patent Laid-Open Publication No. 2003-0017295) is known.

In the present invention, the mixed extract may be mixed at a weight ratio of 1: 6 to 1: 6: 1 to 6: holly basil: peppermint:

The extract mixture of the above-mentioned holly basil, peppermint and walnut tree contained in the composition according to the present invention contains 25 to 50% by weight of the holly basil outpost, 25 to 50% by weight of the mint outpost, 25 to 50% And may be a mixed extract obtained by mixing the above-mentioned holly basil, peppermint, and nematode.

The above-mentioned holly basil, peppermint, and nematode are shredded or shredded, and preferably crushed and pulverized to increase the extraction efficiency.

In the present invention, it is preferable in the present invention to mix the three raw materials together and extract the solvent from the viewpoint of process efficiency. However, in some cases, the same solvent and solvent amount may be used for each of the three raw materials, Then, they may be mixed in an equal amount, or may be mixed in the weight percentage ratio as described above. The extract may be in the form of a concentrate or a lyophilized powder.

The extraction solvent which can be used for obtaining the above-mentioned extraction mixture contained as an active ingredient in the composition for external application for skin according to the present invention may be any conventional extraction solvent, preferably water, 1 to 4 carbon atoms A single pure solvent selected from the group consisting of lower alcohols, butylene glycol, propylene glycol and glycerin, or a mixed solvent of two or more of them may be used, but this is not restrictive in the present invention. The addition amount of the extraction solvent is preferably 1 to 20 times the volume of the total volume of the mixed raw materials.

As the extraction method using the above-mentioned extraction solvent, conventional methods of extracting plants such as hot water extraction, reflux cooling extraction, ultrasonic extraction and supercritical extraction can be used. Specifically, The leaves of the seedlings and seedlings were crushed to prepare powders, which were immersed in the above-mentioned extraction solvent and extracted at room temperature for 24 to 72 hours. Then, the obtained extraction filtrate is filtered and inductively dried to obtain an extractive mixed extract or lyophilized to obtain a powder.

In the present invention, the mixed extract is characterized by containing 0.001 to 30% by weight based on the total weight of the composition for external application for skin. If the amount of the mixed extract is less than 0.001% by weight, the effect of alleviating the skin irritation may become insignificant. On the contrary, if it exceeds 30% by weight, it may be undesirable from the economical viewpoint.

According to the experimental example of the present invention, the inhibitory effect of the inflammatory cytokine increased due to the fine dust was confirmed. As a result, the holy basil, mint, and nematode mixed extracts of the present invention showed excellent inhibitory effect, And the skin irritation inhibition effect was better than that of the control group. From these results, it can be seen that the mixed extract of the present invention can alleviate skin inflammation due to fine dust by inhibiting inflammation-inducing cytokines.

In addition, as a result of confirming the effect of alleviating the stimulation of the skin layer by the fine dust, it was confirmed that the skin extract and epidermal cells damaged by the fine dust stimulation were recovered in the mixed extract of the present invention. From these results, it can be seen that the mixed extract of holly basil, peppermint, and nematode can alleviate the skin damage due to the stimulation by the fine dust, that is, the skin irritation caused by the fine dust.

In the present invention, the composition for external application for skin may be used as a pharmaceutical composition or a cosmetic composition applicable to the skin. In the case of a cosmetic composition, it may be formulated into a form such as a soft lotion, a nutritional lotion, a nutritional essence, , A pack, a foundation, a makeup base, a cleansing, a gel, a lotion and an ointment.

The ingredients contained in the cosmetic composition of the present invention may contain, in addition to the above-mentioned mixed extracts, the components conventionally used in cosmetic compositions as an active ingredient, and may contain conventional ingredients such as stabilizers, solubilizers, vitamins, Adjuvants and carriers.

The pharmaceutical composition of the present invention may be formulated into any formulation known in the art such as cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta or cataplasma.

Further, the preferable dosage of the pharmaceutical composition varies depending on the condition and the weight of the patient, the degree of the disease, the drug form, the administration route and the period, but can be appropriately selected by those skilled in the art. However, for the desired effect, the composition of the present invention is preferably administered at 0.0001 to 100 mg / kg per day, preferably 0.001 to 10 mg / kg per day. External administration may be administered once a day or divided into several doses.

As described above, the holy basil, mint, and nematic mixed extracts of the present invention effectively inhibit the expression of inflammation-inducing cytokines (IL-6, IL-8, IL-1β) overexpressed by various stimuli, Thereby alleviating skin irritation and skin irritation.

1 is a graph showing the results of Experimental Example 1 showing the degree of inhibition of IL-6 gene expression among inflammation-inducing cytokines.
2 is a graph showing the results of Experimental Example 1 showing the degree of inhibition of IL-8 gene expression among inflammation-inducing cytokines.
FIG. 3 is a photograph showing a result of measuring that the skin layer damaged by the fine dust is recovered in the mixed extract treatment.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example  One: Holly Basil , Mints and Dear  Preparation of extraction mixture

A total of 99.9 g of each of 33.3 g of each of the dried holly basil outpast, mint outpost and nymph leaf were pulverized, and the mixture was incubated at room temperature for 24 hours in an aqueous 70 wt.% Ethanol solution as an extraction solvent. ≪ / RTI > The obtained extract was filtered using a filter paper, and the filtrate was concentrated under reduced pressure using a rotary evaporator to obtain a concentrated extract mixture.

Comparative Example  One: Holly Basil , Mints and Dear  Production of single extract

As a comparison, 100 g each of the holly basil outpost, the mint outpost, and the individual raw powder of each leaf of the nymph was placed in a separate container, and the mixture was immersed in 1 L of 70 wt% ethanol aqueous solution as an extraction solvent, And left standing to obtain an extract. The obtained extract was filtered using a filter paper, and the filtrate was concentrated under reduced pressure using a rotary evaporator to obtain concentrated extracts.

Experimental Example  1: Evaluation of anti-inflammatory efficacy

In order to evaluate the anti-inflammatory effect of the mixed extracts, PM10 was administered to IL-6 and IL-8, which are inflammation-inducing cytokines, and their expression levels were measured. More specifically, HaCaT cells, which are keratinocytes, were treated with fine dust, and a final concentration of 10 [mu] g / ml of each of the extracts of Holly basil, mint, and nematode prepared in Comparative Example 1 and the extract mixture obtained in Example 1 / Ml, diluted in the medium, and cultured for 4 hours. Then, mRNA was extracted from the cells, synthesized as cDNA, real-time PCR was performed using a target primer, and the degree of gene expression of the inflammatory cytokine was finally evaluated. The results are shown in FIGS. 1 and 2.

As a result, as shown in FIGS. 1 and 2, when the respective cytokines were induced by the fine dust, the degree of inhibition of the extracts and the mixed extracts could be evaluated, and the mixed extracts showed the highest and synergistic , And showed a remarkable induction inhibitory effect. Therefore, it was confirmed that the mixed extract according to the present invention is a very useful raw material for a cosmetic composition having a higher anti-inflammatory effect than a single extract.

Experimental Example  2: Evaluation of the effect of skin stimulation relaxation

In order to evaluate the effect of mitigation of skin epidermal irritation, artificial skin tissue cultured in three dimensions was used to evaluate the mitigation effect of the skin layer stimulated and damaged by fine dust. 50 占 퐂 / ml of dust fine dust (PM10) was applied to the artificial skin surface, and the mixed extract obtained in Example 1 was treated to a concentration of 10 占 퐂 / ml in the medium. After incubation for 24 hours, the cells were fixed in formalin and paraffin blocks were prepared and stained with H & E. The morphology of the stratum corneum and keratinocytes was measured. The results of the measurement are shown in Fig.

As a result, as shown in FIG. 3, it was confirmed that the stratum corneum of the artificial skin tissue damaged by the fine dust treatment was alleviated. It was also confirmed that the density and shape of keratinocytes in the basal layer were restored. Therefore, it was confirmed that the mixed extract according to the present invention is a very useful raw material for a cosmetic composition having a stimulating effect on the skin layer damaged by stimulation.

Formulation Example  1: Lotion production

Lotions containing the extract mixture obtained in Example 1 were prepared according to the conventional methods according to the compositional components and composition ratios shown in Table 1 below.

ingredient Content (% by weight) Holly basil, mint, nymph blend extract 1.0 glycerin 5.0 ethanol 3.0 Polyoxyethylene hardened castor oil 0.5 Polysorbate bit 80 0.5 Butylene glycol 3.0 Carboxyl vinyl polymer 0.5 Preservative, fragrance Suitable amount Purified water Balance

Formulation Example  2: Manufacture of nutritional essence

A moisture cream containing the extract mixture obtained in Example 1 was prepared in a conventional manner according to the compositional components and composition ratios shown in Table 2 below.

ingredient Content (% by weight) Holly basil, mint, nymph blend extract 1.0 glycerin 7.0 Butylene glycol 3.0 Glyceryl stearate / phage-100 suearate 1.0 Polysorbate 60 0.5 Sorbitan stearate 0.5 Squalane 3.0 Caprylic / capric triglyceride 5.0 Shea butter 2.0 Stearyl alcohol 0.5 Sutearin acid 0.5 Carboxyl vinyl polymer 0.2 Arginine 0.2 Preservative, fragrance Suitable amount Purified water Balance

Formulation Example  3: Moisturizing Cream

A moisturizing cream containing the extract mixture obtained in Example 1 was prepared in a conventional manner according to the compositional components and composition ratios shown in Table 3 below.

ingredient Content (% by weight) Holly basil, mint, nymph blend extract 1.0 glycerin 7.0 Butylene glycol 5.0 Glyceryl stearate / phage-100 suearate 2.0 Polysorbate 60 0.5 Sorbitan stearate 0.5 Phitosqualan 5.0 Jojoba oil 3.0 Caprylic / capric triglyceride 10.0 Aloe butter 3.0 Wax 1.0 Stearyl alcohol 0.5 Beeryl alcohol 0.5 Stearic acid 0.5 Carboxyl vinyl polymer 0.2 Xanthan gum 0.2 Arginine 0.3 Dimethicone 0.5 Beta Glucan 0.2 Preservative, fragrance Suitable amount Purified water Balance

INDUSTRIAL APPLICABILITY As described above, according to the present invention, the cosmetic composition containing the extract of holli basil, peppermint, and nematode as an active ingredient according to the present invention inhibits the expression of inflammation-inducing cytokines (IL-6, IL-8) Stimulation and alleviation of skin irritation ultimately can be effectively applied to the prevention and improvement of skin trouble, improvement of redness, prevention and improvement of dermatitis and the like.

Claims (6)

A composition for external application for skin for skin irritation and skin irritation caused by fine dusts containing an active ingredient of a mixed extract of holly basil, peppermint, and cinnamon.
The composition for external application for skin irritation and skin irritation according to claim 1, wherein the mixed extract is mixed at a weight ratio of 1: 6: 1 to 6: 1 to 6: holly basil: peppermint: .
The composition for external application for skin irritation and skin irritation according to claim 1, wherein the mixed extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof .
[Claim 3] The composition for external application for skin irritation and skin irritation according to claim 1, wherein the mixed extract is contained in an amount of 0.001 to 30% by weight based on the total weight of the composition.
The composition for external application for skin irritation and skin irritation according to claim 1, wherein the mixed extract inhibits skin inflammation by inhibiting the expression of inflammatory cytokines due to fine dusts.
The composition according to claim 1, wherein the composition is at least one selected from the group consisting of a soft lotion, a nutritional lotion, a nutritional essence, a nutrient oil, a moisturizing oil, a nutritional cream, a powder, a pack, a foundation, a makeup base, a cleansing gel, Wherein the external preparation is a cosmetic formulation.

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