KR101988492B1 - Functional cosmetic composition for removing heavy metal and dust containing medicine plant extract and functional cosmetic including the same - Google Patents

Abstract

The present invention relates to a cosmetic composition for eliminating heavy metals or fine dusts, comprising chrysanthemum extracts as active ingredients; and a functional cosmetic product prepared therefrom. In particular, the cosmetic composition comprises a mixture (A+B+C) comprising: a first composition (A); a second composition (B); and chrysanthemum extracts (C). In particular, the first composition (A) comprises, in a predetermined mixing ratio, a first mixture composition, a second mixture composition, a third mixture composition, a fourth mixture composition, and a fifth mixture composition, wherein the first mixture composition contains an equal weight of sea cucumber extracts and Scutellaria Radix extracts, the second mixture composition contains an equal weight of flaxseed extracts and vitamin tree leaf extracts, the third mixture composition contains an equal weight of dark garlic extracts and Leonurus japonicus extracts, the fourth mixture composition contains an equal weight of persimmon leaf extracts and Cimicifuga heracleifolia flower extracts, and the fifth mixture composition contains an equal weight of chestnut leaf extracts and acacia flower extracts. The second composition (B) comprises: Smilacis Rhizoma extracts; and marine algae extracts in which seaweed, kelp, Rheum undulatum LINNE, and sea grape extracts are mixed in an equal weight. The first composition provides benefits in terms of moisturization, antioxidant effects, skin protection, wrinkle reduction, skin-brightening effects, and anti-aging effects, while the second composition provides benefits in terms of eliminating heavy metals and fine dusts, thereby keeping the skin in a healthy state.

Description

TECHNICAL FIELD [0001] The present invention relates to cosmetic compositions for removing heavy metals and fine dusts containing a medicinal extract, and functional cosmetics using the same. BACKGROUND OF THE INVENTION 1. Field of the Invention [0002]

The present invention relates to a first mixed composition comprising a mixture of sea cucumber extract and gold extract at a same weight ratio; A flaxseed extract, and a vitamin tree leaf extract at a same weight ratio; Black garlic extract and mother liquorice extract at a same weight ratio; A persimmon leaf extract, and an equine flower extract in a weight ratio of 4: 1; A first composition (A) for mixing and forming a mixture of a chestnut leaf extract and an acacia flower extract at a same weight ratio; (A + B + C) composition of a second composition (B) for mixing and forming seaweed extracts mixed with sea tangle, seaweed, rhubarb, and mackerel at a same weight ratio and a chrysanthemum extract (C) And to a functional cosmetic composition using the functional cosmetic composition and the functional cosmetic composition using the functional cosmetic composition and the functional cosmetic composition using the composition and the cosmetic composition, .

Particulate matter floating in the air is called dust, and particles of a size that can not be recognized by the naked eye are called fine dust particles. Fine dust is classified according to the size of the particles, and it is named as PM 10, PM 2.5, and PM 1 according to their size, which means the particle size unit of the atmospheric particles.

PM refers to the fine dust that we commonly know as particles with a particle size of 10 μm (micrometer) or less. PM 2.5 is the size of ultra fine dust and has a particle size of 2.5 μm (micrometer) or less. Ultrafine dust PM 1 refers to the concentration of particles having a size of 1 μm (micrometer) or less. The reason why the unit of the range is gradually lowered from PM 10 to PM 1 is because the particle size is smaller and harmful to human body and environment.

The risk of ultrafine dusts containing heavy metals such as lead, cadmium and various chemical substances weakens the metabolism flow of skin and lowers the function of control of sebum and causes dryness, itching, and skin trouble, and heavy metals contained in fine dust Harmful substances such as environmental hormones stimulate the secretion of inflammatory cytokines from the cells and increase the number of white blood cells, resulting in allergic reactions to atopic dermatitis. When the fine dust is absorbed into the skin, it also stimulates the pigment cells to form wrinkles and black spots. When the fine dust is absorbed into the body, it creates a toxic substance called active oxygen, and this active oxygen breaks down the collagen in the skin, thereby reducing the elasticity of the skin, resulting in exogenous aging.

In particular, when fine dust comes into direct contact with the skin located on the outermost part of the body, the skin acts as a barrier between the organism and the environment. If the skin is directly exposed to frequent contamination, Causing problems. Particularly fine dust is 20 times smaller than pores, so it can easily penetrate into human skin. For example, absorption of substances such as chloroform, a toxic substance, into the skin is considered to be the same as inhalation by the respiratory tract, which is the main site for absorbing substances from environmental pollution.

In connection with the present invention, Korean Patent Registration No. 10-1850313 (Registration Date 2018.04.13) 'cosmetic composition for removing heavy metals or fine dusts containing chrysanthemum extract as an active ingredient'; Korean Patent Laid-Open No. 10-2018-0020574 (Publication date 2018.02.28) 'Cosmetic composition for removing heavy metals or fine dusts containing as an active ingredient an extract of Panax ginseng and Indian rubber tree'; Korean Patent Publication No. 10-2018-0020573 (Publication date 2018.02.28) 'Cosmetic composition for removing heavy metals or fine dusts containing Panda rubber tree and skin extract as an active ingredient'; Korean Patent Laid-Open No. 10-2018-0020571 (Publication date 2018.02.28) 'Cosmetic composition for removing heavy metals or fine dusts containing an extract of Indian Borax Seeds as an active ingredient'; Korean Registered Patent No. 10-1643696 (Registration Date 2016.07.22) 'Cosmetic composition for removing heavy metals and fine dusts containing bean sprouts'; Korean Registered Patent No. 10-1703928 (Registration date 2017.02.01) 'Cosmetic composition for removing fine dust and heavy metals'; Korean Patent Registration No. 10-1786861 (Registration Date 2017.10.11) discloses a technique for a cosmetic composition for removing heavy metals and fine dusts.

Conventionally disclosed cosmetics or cosmetics are disadvantageous in that they have a complex function of moisturizing, antioxidation, skin protection and wrinkle improvement, whitening and antiaging, in addition to the function of removing heavy metals and fine dusts.

Korean Registered Patent No. 10-1850313 (Registration date 2014.04.13) Korean Patent Publication No. 10-2018-0020574 (Publication date 2018.02.28) Korean Patent Publication No. 10-2018-0020573 (Publication date 2018.02.28) Korean Patent Publication No. 10-2018-0020571 (published date 2018.02.28) Korean Registered Patent No. 10-1643696 (Registered on July 22, 2016) Korean Registered Patent No. 10-1703928 (Registration date 2017.02.01) Korean Registered Patent No. 10-1786861 (Registration date 2017.10.11)

The present invention relates to a first mixed composition comprising a mixture of sea cucumber extract and gold extract at a same weight ratio; A flaxseed extract, and a vitamin tree leaf extract at a same weight ratio; Black garlic extract and mother liquorice extract at a same weight ratio; A persimmon leaf extract, and an equine flower extract in a weight ratio of 4: 1; A first composition (A) for mixing and forming a mixture of a chestnut leaf extract and an acacia flower extract at a same weight ratio; (A + B + C) composition of a second composition (B) for mixing and forming seaweed extracts mixed with sea tangle, seaweed, rhubarb, and mackerel at a same weight ratio and a chrysanthemum extract (C) To provide a functional cosmetic composition which combines moisturizing, antioxidation, skin protection and wrinkle improvement, whitening, anti-aging function, and heavy metal and fine dust removing function by the above-mentioned second composition and chrysanthemum extract, and a functional cosmetic composition using the same The invention of the invention.

In order to achieve the above object,

In the present invention, the sea cucumber and ethanol are mixed at a weight ratio of 1:10 to 12, and the mixture is extracted from the shaker culture at 120 to 160 rpm at 24 to 26 ° C for 65 to 75 hours. The extracted extract is centrifuged at 7,500 to 8,500 rpm The supernatant obtained by centrifugation for 15 to 25 minutes was filtered with a filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA using a vacuum rotation evaporator Sea cucumber extract,

The mixture of gold and methanol was mixed at a weight ratio of 1: 4 to 6, and the mixture was refluxed at 65 to 75 ° C for 20 to 30 hours. The extracted extract was centrifuged at 3,500 to 4,500 rpm for 4 to 6 minutes to obtain a supernatant The mixture was filtered with a filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA with a vacuum rotation evaporator to obtain a first mixture composition a1) 0.5 to 60.0 wt%;

Flaxseed powder and ethanol are mixed at a weight ratio of 1:10 to 15, and then the mixture is dipped at 50 to 80 ° C for 2 to 10 hours to extract. The extracted extract is filtered with a filter paper, For 5 to 7 hours under reduced pressure using a vacuum rotation evaporator,

Vitamin tree leaves and ethanol were mixed at a weight ratio of 1:15 to 25 and then dipped at room temperature for 22 to 26 hours to separate the supernatant and the precipitate. The same extract was repeated three times to filter paper 0.5 to 60.0 wt% of a second mixed composition (a2) prepared by mixing and filtering the filtrate, and concentrating the filtrate at reduced pressure with a vacuum rotation evaporator at 58 to 63 ° C for 5 to 7 hours, ;

The mixture of black garlic and distilled water was mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken for 1 to 5 hours under the conditions of 100 ° C and 100 to 140 rpm. The extracted extract was filtered with a filter paper, The extract of black garlic obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of ~ 0.3 mPA,

The motherwort and distilled water are mixed at a weight ratio of 1: 5 to 30, and the mixture is shaken for 1 to 5 hours at 100 ° C and 100 to 140 rpm. The extracted extract is centrifuged at 2,000 to 4,000 rpm for 10 to 30 minutes After centrifuging, the supernatant was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA, (A3) 0.5 to 60.0 wt%;

The mixture of persimmon leaves and ethanol was mixed at a weight ratio of 1: 5 ~ 30, and the mixture was reflux cooled for 2 ~ 6 hours at 42 ~ 48 ℃. The extract was centrifuged at 11,000 ~ 13,000 rpm for 20 ~ The supernatant was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.

The ration flowers and 70% ethanol were mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken at 23 to 28 ° C and 80 to 150 rpm for 40 to 55 hours. The obtained extract was filtered with a filter paper 0.5 to 60.0 wt% of a fourth mixed composition (a4) prepared by concentrating the filtrate under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA in the same weight ratio;

Chestnut leaf and ethanol were mixed at a weight ratio of 1:10 to 15, and then the mixture was immersed at room temperature for 22 to 26 hours to separate the supernatant and the precipitate. The same procedure was repeated three times, and the extracted extract was filtered with a filter paper , Chestnut leaf extract obtained by concentrating the filtrate under reduced pressure at 58 to 63 ° C for 5 to 7 hours with a vacuum rotation evaporator,

After drying and finishing the Acacia (Robiniapseudoacacia) flower, the powder is mixed with the purified water at a weight ratio of 1:35 to 45, and then extracted at 85 to 99 ° C for 5 to 7 hours. The extracted extract is filtered with filter paper , And the filtrate was centrifuged at 2,000 ~ 4,000 rpm for 10 ~ 30 minutes in a centrifuge. The supernatant was filtered and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA (A1 + a2 + a3 + a4 + a5) of 0.5 to 60.0 wt% of a fifth mixed composition (a5) prepared by mixing the obtained acacia flower extract in a weight ratio of 100.0 wt% ) 50.0 to 90.0 wt%

The mixture of soil and ethanol was mixed at a weight ratio of 1: 5 ~ 30, and the mixture was immersed at 50 ~ 80 ℃ for 2 ~ 10 hours. The extracted extract was filtered with a filter paper to obtain a filtrate of 0.1 ~ 0.3 mPA 20.0 to 80.0 wt% of the extract obtained by concentrating the extract under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the conditions;

Seaweed mixed with seaweed, kelp, rhubarb, and mackerel were mixed at a weight ratio of 58 to 62 ° C for 10 to 14 hours in a hot air drier, and the pulverized dried powder and distilled water were mixed at a weight ratio of 1:15 to 20, The resulting extract was filtered with a filter paper and concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPa 5.0 to 30.0 wt% of a second composition (B) of 100.0 wt% composed of a mixture of 20.0 to 80.0 wt% of the obtained seaweed extract,

The chrysanthemum is dried and powdered. Chrysanthemum powder and distilled water are mixed at a weight ratio of 1:10 to 12 times, refluxed at 75 to 85 ° C for 2 to 4 hours, (A +) mixture of 5.0 to 20.0 wt% of the chrysanthemum extract (C) obtained by concentration under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA with a vacuum rotation evaporator using a filter paper, B + C). The present invention provides a cosmetic composition for removing heavy metals and fine dust, which comprises the extract of Pharmacopoeia as an active ingredient.

Also provided is a functional cosmetic composition prepared by adding a cosmetic composition in an amount of 0.1 to 50.0 wt% based on the total weight of the cosmetic.

The cosmetic composition for removing heavy metals and fine dusts containing the extract of the medicinal substance according to the present invention as an active ingredient and the functional cosmetic composition using the same have the following effects.

first. Sea cucumber extract, and golden extract at a same weight ratio; A flaxseed extract, and a vitamin tree leaf extract at a same weight ratio; Black garlic extract and mother liquorice extract at a same weight ratio; A persimmon leaf extract, and an equine flower extract in a weight ratio of 4: 1; Chestnut leaf extract, and acacia flower extract in a weight ratio of 1: 1 to 5: 1 by weight of the first composition, wherein the first composition is mixed and formed at a predetermined blending ratio to provide moisturizing, antioxidant, skin protection and wrinkle improvement, whitening and antiaging effect.

second. Soil extract; A second composition which is prepared by mixing marine algae, kelp, rhubarb, and mackerel at a same weight ratio, and a chrysanthemum extract to remove heavy metals and fine dusts.

third. By using the natural extract, it is possible to provide a complex function useful for skin by the first composition and the second composition which constitute the cosmetic composition and which is free of skin irritation and stable to the skin.

fourth. Acanthopanax extract; Moss extract; Baechutong extract; By additionally adding the third composition composed of the grapefruit seed extract to the cosmetic composition, it is possible to use the third composition having excellent antimicrobial and antioxidative function more effectively and cleanly and safely because of blocking of the bacterial infection and the like, It has an advantage that it can be extended.

Hereinafter, the technical contents of the above description will be described in detail.

As described above, the cosmetic composition for removing heavy metals and fine dusts containing the extract of the medicinal substance according to the present invention as an active ingredient,

The mixture of sea cucumber and ethanol was mixed at a weight ratio of 1:10 to 12, and the mixture was extracted with shaking incubator at 120 to 160 rpm at 24 to 26 ° C for 65 to 75 hours. The extracted extract was centrifuged at 7,500 to 8,500 rpm for 15 to 25 Min. The supernatant obtained by centrifuging was filtered with a filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPa by a vacuum rotation evaporator.

The mixture of gold and methanol was mixed at a weight ratio of 1: 4 to 6, and the mixture was refluxed at 65 to 75 ° C for 20 to 30 hours. The extracted extract was centrifuged at 3,500 to 4,500 rpm for 4 to 6 minutes to obtain a supernatant The mixture was filtered with a filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA with a vacuum rotation evaporator to obtain a first mixture composition a1) 0.5 to 60.0 wt%;

Flaxseed powder and ethanol are mixed at a weight ratio of 1:10 to 15, and then the mixture is dipped at 50 to 80 ° C for 2 to 10 hours to extract. The extracted extract is filtered with a filter paper, For 5 to 7 hours under reduced pressure using a vacuum rotation evaporator,

Vitamin tree leaves and ethanol were mixed at a weight ratio of 1:15 to 25 and then dipped at room temperature for 22 to 26 hours to separate the supernatant and the precipitate. The same extract was repeated three times to filter paper 0.5 to 60.0 wt% of a second mixed composition (a2) prepared by mixing and filtering the filtrate, and concentrating the filtrate at reduced pressure with a vacuum rotation evaporator at 58 to 63 ° C for 5 to 7 hours, ;

The mixture of black garlic and distilled water was mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken for 1 to 5 hours at 100 DEG C and 100 to 140 rpm. The extracted extract was filtered with a filter paper, The extract of black garlic obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of ~ 0.3 mPA,

The motherwort and distilled water are mixed at a weight ratio of 1: 5 to 30, and the mixture is shaken for 1 to 5 hours at 100 ° C and 100 to 140 rpm. The extracted extract is centrifuged at 2,000 to 4,000 rpm for 10 to 30 minutes After centrifuging, the supernatant was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA, (A3) 0.5 to 60.0 wt%;

The mixture of persimmon leaves and ethanol was mixed at a weight ratio of 1: 5 ~ 30, and the mixture was reflux cooled for 2 ~ 6 hours at 42 ~ 48 ℃. The extract was centrifuged at 11,000 ~ 13,000 rpm for 20 ~ The supernatant was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.

The ration flowers and 70% ethanol were mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken at 23 to 28 ° C and 80 to 150 rpm for 40 to 55 hours. The obtained extract was filtered with a filter paper 0.5 to 60.0 wt% of a fourth mixed composition (a4) prepared by concentrating the filtrate under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA in the same weight ratio;

Chestnut leaf and ethanol were mixed at a weight ratio of 1:10 to 15, and then the mixture was immersed at room temperature for 22 to 26 hours to separate the supernatant and the precipitate. The same procedure was repeated three times, and the extracted extract was filtered with a filter paper , Chestnut leaf extract obtained by concentrating the filtrate under reduced pressure at 58 to 63 ° C for 5 to 7 hours with a vacuum rotation evaporator,

After drying and finishing the Acacia (Robiniapseudoacacia) flower, the powder is mixed with the purified water at a weight ratio of 1:35 to 45, and then extracted at 85 to 99 ° C for 5 to 7 hours. The extracted extract is filtered with filter paper , And the filtrate was centrifuged at 2,000 ~ 4,000 rpm for 10 ~ 30 minutes in a centrifuge. The supernatant was filtered and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA (A1 + a2 + a3 + a4 + a5) of 0.5 to 60.0 wt% of a fifth mixed composition (a5) prepared by mixing the obtained acacia flower extract in a weight ratio of 100.0 wt% ) 50.0 to 90.0 wt%

The mixture of soil and ethanol was mixed at a weight ratio of 1: 5 ~ 30, and the mixture was immersed at 50 ~ 80 ℃ for 2 ~ 10 hours. The extracted extract was filtered with a filter paper to obtain a filtrate of 0.1 ~ 0.3 mPA 20.0 to 80.0 wt% of the extract obtained by concentrating the extract under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the conditions;

Seaweed mixed with seaweed, kelp, rhubarb, and mackerel were mixed at a weight ratio of 58 to 62 ° C for 10 to 14 hours in a hot air drier, and the pulverized dried powder and distilled water were mixed at a weight ratio of 1:15 to 20, The resulting extract was filtered with a filter paper and concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPa 5.0 to 30.0 wt% of a second composition (B) of 100.0 wt% composed of a mixture of 20.0 to 80.0 wt% of the obtained seaweed extract,

The chrysanthemum is dried and powdered. Chrysanthemum powder and distilled water are mixed at a weight ratio of 1:10 to 12 times, refluxed at 75 to 85 ° C for 2 to 4 hours, (A +) mixture of 5.0 to 20.0 wt% of the chrysanthemum extract (C) obtained by concentration under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA with a vacuum rotation evaporator using a filter paper, B + C).

The cosmetic composition according to the present invention is a first mixed composition comprising a mixture of sea cucumber extract and gold extract at a same weight ratio; A flaxseed extract, and a vitamin tree leaf extract at a same weight ratio; Black garlic extract and mother liquorice extract at a same weight ratio; A persimmon leaf extract, and an equine flower extract in a weight ratio of 4: 1; A first composition (A) for mixing and constituting a fifth mixture composition prepared by mixing a chestnut leaf extract and an acacia flower extract in a weight ratio,

Soil extract; A second composition (B) for mixing and forming seaweed supplements mixed with seaweed, kelp, rhubarb, and mung bean at a same weight ratio,

It is a functional cosmetic composition having a moisturizing, antioxidant, skin protection and wrinkle improvement, whitening, anti-aging effect, and a function of removing heavy metals and fine dusts.

≪ First composition >

Wherein the first composition comprises 0.5 to 60.0 wt% of a first mixed composition comprising the sea cucumber extract and the gold extract in a weight ratio; 0.5 to 60.0 wt% of a second mixture composition comprising flaxseed extract and vitamin tree leaf extract in a weight ratio of 1: 1; A mixture of black garlic extract and mother liquorice extract in a weight ratio of 0.5 to 60.0 wt%; Persimmon leaf extract, and horse ridberry flower extract in a weight ratio of 0.5 to 60.0 wt%; Chestnut leaf extract and acacia flower extract in a weight ratio of 0.5 to 60.0 wt%; and a specific formulation thereof is shown in Table 1 below.

Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 The first mixed composition 10.0 wt% 25.0 wt% 15.0 wt% 30.0 wt% 20.0 wt% The second mixed composition 20.0 wt% 10.0 wt% 25.0 wt% 15.0 wt% 20.0 wt% Third mixed composition 30.0 wt% 20.0 wt% 10.0 wt% 25.0 wt% 20.0 wt% Fourth mixed composition 15.0 wt% 30.0 wt% 20.0 wt% 10.0 wt% 20.0 wt% Fifth mixed composition 25.0 wt% 15.0 wt% 30.0 wt% 20.0 wt% 20.0 wt% Sum 100.0 wt% 100.0 wt% 100.0 wt% 100.0 wt% 100.0 wt%

1. First mixed composition

The first mixed composition has a moisturizing function, and is mixed with the sea cucumber extract and the gold extract at a weight ratio of 1: 1.

Sea cucumber is a generic term for sea cucumbers belonging to the sea cucumber river. There are three kinds of sea cucumbers in Korea, red ginseng, blue ginseng and black ginseng. Sea cucumbers act as amino acid and iron (Fe), phosphorus (P) and bile components such as cysteine, histidine and lysine to prevent anemia and liver. Taurine (Taurine) is contained in the gallbladder and liver to detoxify the function of strengthening, cholesterol lowering, heart function and improve vision is said to be effective in the recovery.

The raw sea cucumber contains water, protein, carbohydrate, ash, calcium, phosphorus, iron, lysine, vitamin 1, B2, essential amino acid, erythrocyte thionin, glutamic acid, glycine, tryptophan and taurine. Sea cucumber extracts from sea cucumbers contain chondroitin, a type of mucopolysaccharide. Chondroitin plays a role in accumulating moisture and nutrients in the skin. Chondroitin sulfate is a major component of viscous polysaccharides and is a mucopolysaccharide found in animal cartilage, nervous tissue and intercellular tissue. Chondroitin sulfate does not exist in vivo but is bound to proteins to form a chondroitin sulfate protein complex. It is the basis for living somatic cells in vivo, such as collagen, elastin, hyaluronic acid, etc., It gives resilience. Chondroitin sulfate, which is contained in sea cucumber extract, keeps moisturizing function by storing moisture in the skin. It also stores a large amount of amino acid, protein and iodine ingredient to save skin wrinkles, prevent aging, skin whitening efficacy, .

The sea cucumber extracts were prepared by mixing sea cucumber and ethanol at a weight ratio of 1:10 to 12, and then extracting the extracts at 24 to 26 ° C for 65 to 75 hours at 120 to 160 rpm in a shaking incubator. The extracted extracts were centrifuged at 7,500 to 8,500 rpm The supernatant obtained by centrifugation for 15 to 25 minutes was filtered with a filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA using a vacuum rotation evaporator .

As a specific example of the above-mentioned sea cucumber extract, 1,000 g of sea cucumber is placed in 10 L of 50% ethanol and extracted at 160 rpm at 26 DEG C for 70 hours in a shaking incubator to obtain an extract. The extract is centrifuged at 7,000 rpm for 30 minutes in a centrifuge. The supernatant obtained by centrifugation is filtered with filter paper (Whatman No. 3 filter paper). The filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours by a vacuum rotation evaporator to obtain 1 L of an extract.

Golden (Scutellaria baicalensis) is a perennial plant belonging to the botanical tree of the botanical plant. It is called money, a little bit, and an organism, and it uses taste, and the property is cold and nontoxic. It mainly uses roots. Gold is known as a potent pharmacologic crop and has been used as an alternative medicine for anti-inflammatory, anti-cancer and soothing effects.

The gold extract was prepared by mixing gold and methanol at a weight ratio of 1: 4 to 6, refluxing the extract at 65 to 75 ° C for 20 to 30 hours, centrifuging the extracted extract for 4 to 6 minutes at 3,500 to 4,500 rpm The supernatant is filtered with a filter paper, and the filtrate is concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA using a vacuum rotation evaporator.

As a specific example of the gold extract, dried gold is finely pulverized, and 1,000 g of gold fine powder and 5 L of 70% ethanol are mixed, followed by reflux extraction at 72 ° C for 15 hours.

The extract is centrifuged at 3,500 rpm for 10 minutes, and the supernatant is collected and filtered with filter paper (Whatman No. 3 filter paper). The filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours by a vacuum rotation evaporator to obtain 1 L of an extract.

At this time, the obtained extract was dried for 3 days using a freeze dryer, and then pulverized to a size of 40 mesh to prepare a golden extract.

2. Second Mixture Composition

The first mixed composition has an antioxidant function and is prepared by mixing 1: 1 weight ratio of flaxseed extract and vitamin tree leaf extract.

The flaxseed is Linum usitatissimum and is a seed of the flax (Linaceae) plant. Flaxseed contains vitamins and essential amino acids. However, there are three representative beneficial ingredients that are rich in omega-3 fatty acids, lignans and dietary fiber.

Lignans have antioxidant properties and have very useful antioxidant activities in human body. It has excellent anticancer, anti-cleavage, and anti-toxicity and has a function of strongly inhibiting already formed tumors.

The flaxseed extract is prepared by mixing the flaxseed powder with ethanol at a weight ratio of 1:10 to 15, and then dipping the mixture at 50 to 80 ° C for 2 to 10 hours. The extracted extract is filtered with a filter paper, And obtained by concentrating under reduced pressure with a vacuum rotation evaporator at 58 to 63 ° C for 5 to 7 hours.

Specific examples of the flaxseed extract include,

After drying, 1,000 g of the pulverized flaxseed powder is immersed in 10 L of 95% ethanol, and then heated at 62 DEG C for 3 hours. The extract was filtered with a filter paper, and the filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

Vitamin tree (Hippophae rhamnoides L.) is a deciduous shrub belonging to Elaeagnacease, origin of China, Mongolia and others. Vitamin tree leaves and fruits contain vitamins and amino acids, and have been reported to have immunity and anti-inflammatory effects. Fruits contain polyphenols, tocopherols, carotenoids, flavonoids, and leaves are known to contain quercetin, gallic acid, tannin, and other physiologically active substances.

The vitamin tree leaf extract is prepared by mixing vitamin tree leaves and ethanol in a weight ratio of 1:15 to 25 and then immersing the mixture at room temperature for 22 to 26 hours to separate the supernatant and precipitate, Filtered through filter paper, and the filtrate is obtained by concentration under reduced pressure at 58 to 63 ° C for 5 to 7 hours using a vacuum rotation evaporator.

Specific examples of the vitamin tree leaf extract include,

1,000 g of vitamin tree leaves are added to 10 L of 50% ethanol, and after immersing at room temperature for 25 hours, the supernatant is separated from the precipitate. The filtrate was extracted by repeating the same procedure three times, and the filtrate was concentrated under reduced pressure at 62 캜 and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

3. Third Mixture Composition

The third mixed composition has a skin protection and a wrinkle-reducing function, and is mixed at a ratio of 1: 1 by weight of black garlic extract and motherwort extract.

When garlic is aged in a high-temperature thermostat for a certain period of time, garlic is changed into black by its own components and enzymes, etc., and this is called "black garlic".

As the raw garlic turns into black garlic, the unstable and odorous components of raw garlic are changed into a more stable and odorless water-soluble substance and organic sulfur compounds such as S-allylcysteine (SAC), S-allylmercaptocystein (SAMC) and tetrahydro- soluble substances such as diallyl sulfide (DAS), triallyl sulfide, diallyl disulfide (DADS) and diallyl polysulfides. Fructosyl-arginine inhibits the production of thiobarbituric acid reactive substances (TBARS) in the liver and cell damage caused by oxidized LDL. .

The black garlic extract is prepared by mixing black garlic and distilled water at a weight ratio of 1: 5 to 30, shaking the mixture at 100 DEG C and 100 to 140 rpm for 1 to 5 hours, filtering the extracted extract with a filter paper, , And the filtrate is obtained by concentration under reduced pressure using a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours.

More specifically, 1000 g of black garlic was added to 10 L of distilled water, and the mixture was shaken at 100 rpm and 120 rpm for 2 hours. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

The motherwort (Leonurus japonicus H.) is a two-year-old herb belonging to Lamiaceae. As the main components, leucine, leonuridine, stachynose, stachydrine, flavonoid, rutin, protocatechol, benzoic acid, vitamin A and fat were found to be alkaloids.

In addition to activating the blood, the motherwort juice also removes toxic blood in the body. In addition, when the blood pressure is high and the blood pressure is high, It is said to be used to treat uterine hemorrhage, hematuria, hemorrhoids, hemorrhoids, and urinary incontinence when it is dizziness and high fever due to hemorrhage and severe pain.

The motherwort extract was prepared by mixing motherwort and distilled water at a weight ratio of 1: 5 to 30, and then shaking the mixture at 100 ° C and 100 to 140 rpm for 1 to 5 hours. The extracted extract was centrifuged at 2,000 to 4,000 rpm for 10 After centrifugation for 30 minutes, the supernatant was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPa use.

More specifically, 1,000 g of dried mother liquorice powder was added to 15 L of distilled water, shaken at 100 DEG C for 2 hours, and then centrifuged at 3,000 rpm for 15 minutes to obtain a supernatant from which the debris was removed. The supernatant was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure for 5 hours under vacuum of 0.2 mPA using a vacuum rotation evaporator to obtain 1 L of an extract.

4. Fourth Mixture Composition

The fourth mixed composition has a whitening function, and is mixed with the persimmon leaf extract and the ritual flower extract in a weight ratio of 1: 1.

(+) - catechin, phloroglucinol, (+) - catechin-3-gallate, (+) - gallocatechin, and (+) - catechin as a mixture of various condensed tannins. It is known that falvan-3-ol compounds such as gallocatechin-3-gallate and condensed tannin belong to proanthocyanidin. Tannin compounds of persimmon leaves have an inhibitory effect on blood pressure rise, and condensed tannins have an effect of inhibiting free radicals of active oxygen. It also has antioxidant activity, anticancer and antimutagenic effects.

Persimmon leaves were mixed with persimmon leaves and ethanol at a weight ratio of 1: 5 ~ 30, and then subjected to reflux cooling extraction at 42 to 48 ° C for 2 to 6 hours. The extracted extract was centrifuged at 11,000 to 13,000 rpm for 20 to 40 minutes The supernatant is separated and the supernatant is filtered with a filter paper, and the filtrate is concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA. .

More specifically, 1,000 g of the persimmon leaf powder obtained by pulverizing and drying the persimmon leaves is added to 10 L of 80% ethanol, and the mixture is refluxed and cooled at 45 캜 for 6 hours. The obtained extract is centrifuged at 11,000 rpm for 40 minutes using a centrifuge to take the supernatant. The supernatant was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure for 5 hours under vacuum of 0.2 mPA using a vacuum rotation evaporator to obtain 1 L of an extract.

Cimicifugae Rhizoma is a rootstock of Cimicifuga heracleifolia Komarov or Ranunculaceae. It is taken in the summer and autumn, and its beard roots are removed, dried in the sun, dried, and grown in Korea. A variety of 9,19-cyclolanostane glycosides have been found in horse riding, while Acetylshengmanol xyloside and cimicifugoside H-1 are found in horse riding. Other compounds such as cimicifugin, khellol, amminol, caffeic acid and ferulic acid are known.

The horse riding extract was mixed with 70% ethanol at a ratio of 1: 5 ~ 30 by weight, followed by shaking at 23 ~ 28 ° C and 80 ~ 150 rpm for 40 ~ 55 hours. Filter paper ), And the filtrate is obtained by concentrating the filtrate under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.

More specifically, 1,000 g of crushed horse powder is added to 20 L of 70% ethanol and shaken out at 26 DEG C and 120 rpm for 50 hours. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

5. Fifth mixed composition

The fifth mixed composition has an anti-aging function and is mixed with the chestnut leaf extract and the acacia flower extract at a weight ratio of 1: 1.

Chestnut (Castanea crenata) is a deciduous broad-leaved arboreous tree belonging to oak.

Studies on chestnut (Castanea crenata) leaves are currently being carried out in various ways, but the range is not so wide.

The chestnut leaf extract was prepared by mixing chestnut leaf and ethanol at a weight ratio of 1:10 to 15, and then dipping at room temperature for 22 to 26 hours to separate the supernatant and the precipitate. The extracted extract was filtered three times, The filtrate is obtained by concentrating the filtrate under reduced pressure at 58 to 63 ° C for 5 to 7 hours using a vacuum rotation evaporator.

Specific examples of the chestnut leaf extract include,

1,000 g of chestnut leaves are added to 10 L of 70% ethanol and then immersed at room temperature for 24 hours. The supernatant is separated from the supernatant and the supernatant is obtained, and this process is repeated twice. The supernatant is mixed and filtered with a filter paper.

The filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

Acacia (Robinia pseudoacacia) is a deciduous tree belonging to rosewood Congo.

The moisture content of the acacia petals was 86.60% and when dried, crude protein was 24.55%, crude ash was 8.51%, total sugar was 30.97%, ascorbic acid About 16%, and tannic acid is about 15%, which is known to have a relatively high content of sugar, protein, and ascorbic acid.

The acacia flower extract was prepared by mixing dried acacia (Robinia pseudo acacia) flowers with ethanol at a weight ratio of 1: 5 to 55, and then repeatedly extracted three times at 25 to 78 ° C for 4 to 5 hours. The resulting extract was filtered The filtrate obtained by concentrating the filtrate under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA with a vacuum rotation evaporator is used.

More specifically, 1,000 g of dried acacia flowers are finely cut and mixed with 10 L of 50% ethanol, and the mixture is repeatedly extracted three times at 25 캜 for four hours. The obtained extract is filtered with a filter paper. The filtered extract was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

≪ Second composition >

Wherein the second composition comprises 20.0-80.0 wt% 20.0 ~ 80.0 wt% of seaweed extracts mixed with seaweed, kelp, rhubarb, and mung bean at the same weight ratio, and has the function of removing heavy metals and fine dusts.

Specific formulations of the second composition are shown in Table 2 below.

Formulation Example 1 Formulation Example 2 Formulation Example 3 Tobogyung extract 30.0 wt% 45.0 wt% 70.0 wt% Algae extract 70.0 wt% 55.0 wt% 30.0 wt% Sum 100.0 wt% 100.0 wt% 100.0 wt%

1. soil extract

Smilacis glabrae Rhizoma (SG) is a medicinal herb that refers to the rhizomes of Cynomolgus sinensis. The Cheongmaidae (Smilax china L.) is a shrub, and rhizomes extend sideways.

It has been used as a therapeutic drug for chronic skin diseases, gonorrhea, diuretic, and toxic skin diseases. Saponin such as parillin, smilacin, etc. Are reported as main components of soil bokyong. It contains ophiopogonin, a glycoside separated from roots and mucous materials, and plays an important role in protecting organ from immune enhancement and various bacterial infections.

The soil extract is prepared by mixing soil bacterium and ethanol at a weight ratio of 1: 5 to 30, followed by immersion at 50 to 80 ° C for 2 to 10 hours, extracting the extracted extract with a filter paper, Which is obtained by concentration under reduced pressure using a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours.

More specifically, 1,000 g of the shade and crushed red ginseng powder of Smilacis glabrae Rhizoma (SG) is immersed in 8 L of 95% ethanol and extracted at 65 ° C for 5 hours. The obtained extract was filtered through a filter paper (Whatman No. 2), and the filtrate was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

2. Algae extract

Seaweeds are known to produce toxic or growth-limiting substances for use in competition with other organisms and to produce substances of self-defense against living creatures that attach to surfaces, making them valuable for the development of natural antibiotics. From past to present, many studies have revealed the results of physiological activities such as antioxidation, antibacterial, anticancer, anti-inflammation and anti-blood coagulation of seaweed.

In the present invention, seaweeds such as seaweed, kelp, moxa, and rhubarb are mixed at the same weight ratio.

The seaweed extracts were prepared by mixing seaweed, kelp, rhubarb, and mungbean at a weight ratio of 58:62, followed by drying in a hot air drier for 10 to 14 hours. The dried powder and distilled water were mixed at a weight ratio of 1:15 to 20 The resulting extract was filtered through a filter paper, and the filtrate was vacuum-evaporated for 5 to 7 hours under the condition of 0.1 to 0.3 mPA. To obtain a solution.

Specific examples of the algae extract include,

Dried seaweed, kelp, kelp, and rhubarb were dried at 60 ° C for 12 hours in a hot air dryer, and then 1,000 g of dried powder mixed with dried seaweed, kelp, moxa, and rhubarb were mixed with 15 L of distilled water Then, the extract which has been heated and extracted at 100 ° C for 2 hours is filtered with a filter paper.

The filtered extract was concentrated under reduced pressure at 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

<Chrysanthemum extract>

Asteraceae plants have been used for edible and medicinal materials since ancient times and have been used for a long time due to pharmacological actions such as fever, detoxification, headache, diuretic, infectious infection and colitis in oriental herbs. Has come.

The chrysanthemum extract is prepared by drying the chrysanthemum and then powdering it. The chrysanthemum powder and distilled water are mixed at a weight ratio of 1:10 to 12 times, refluxed at 75 to 85 ° C for 2 to 4 hours, Is filtered with a filter paper and the filtrate is concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA using a vacuum rotation evaporator.

More specifically,

The chrysanthemum powder and distilled water were mixed at a weight ratio of 1: 10, and the mixture was refluxed at 80 ° C for 3 hours. The extract was filtered with a filter paper, and the filtrate was filtered through a vacuum rotation evaporator mPA under reduced pressure for 6 hours.

The first composition constituting the cosmetic composition for removing heavy metals and fine dust according to the present invention is a composition having a function of moisturizing, antioxidation, skin protection and wrinkle improvement, whitening, antiaging and containing a combination of sea cucumber extract and gold extract in a weight ratio 0.5 to 60.0 wt% of a mixture of flaxseed extract and vitamin tree leaf extract in a weight ratio of 0.5 to 60.0 wt%, a mixture of black garlic extract and motherweed extract in a weight ratio of 0.5 to 60.0 wt% 0.5 to 60.0 wt% of the fourth mixture composition prepared by mixing the persimmon leaf extract and the ritual flower extract in the same weight ratio, 0.5 to 60.0 wt% of the fifth mixture composition prepared by mixing the chestnut leaf extract and the acacia flower extract in the same weight ratio, .

Specifically,

20.0 wt% of the first mixed composition, 20.0 wt% of the second mixed composition, 20.0 wt% of the third mixed composition, 20.0 wt% of the fourth mixed composition, and 20.0 wt% of the fifth mixed composition.

The second composition constituting the cosmetic composition for removing heavy metals and fine dust according to the present invention has a function of removing heavy metals and fine dusts and is composed of 20.0 ~ 80.0 wt% of the extract of Toyohaku and 20.0 ~ 80.0 wt% of seaweed extract .

Specifically,

It is composed of a mixture of 45.0 wt% of extract of Toyohaku and 55.0 wt% of seaweed extract.

The cosmetic composition for removing heavy metals and fine dust according to the present invention contains 50.0 to 90.0 wt% of the first composition (A), 5.0 to 30.0 wt% of the second composition (B) and 5.0 to 20.0 wt % &Lt; / RTI &gt;

More specifically, it is composed of 60.0 wt% of the first composition of Example 1, 30.0 wt% of the second composition of Example 2, and 10.0 wt% of the chrysanthemum extract.

The cosmetic composition for removing heavy metals and fine dust according to the present invention is prepared by adding a mushroom extract to the total weight of the second composition (B) in an amount of 0.1 to 20.0 wt% in order to further improve the heavy metal removal function.

More specifically, it is prepared by mixing 90.0 wt% of the second composition (B) and 10.0 wt% of the mugwort extract, and is composed of 80.0 ~ 99.9 wt% of the second composition (B) and 0.1 ~ 20.0 wt% do.

The mugwort extract was prepared by drying the mugwort extract at 58 to 62 ° C. for 10 to 14 hours in a hot air drier, mixing the pulverized dried powder and distilled water at a weight ratio of 1:15 to 20, heating and heating at 80 to 90 ° C. for 1 to 3 hours To obtain an extract, which is obtained by concentrating the extract at a reduced pressure of 0.1 to 0.3 mPA with a vacuum rotation evaporator for 5 to 7 hours.

More specifically,

The mushroom was dried at 60 ° C for 12 hours in a hot air dryer, and the pulverized dried powder and distilled water were mixed at a weight ratio of 1:15 and heated at 80 to 90 ° C for 1 to 3 hours to obtain an extract. And the resultant is obtained by concentration under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of -0.3 mPA.

The bear is a perennial brown alga belonging to Laminariaceae (Laminariaceae). The genus Ecklonia in the kelp is classified again into three species: Ecklonia cava Kjellman, EckloniakuromeOkamura and EckloniastoloniferaOkamura.

The cosmetic composition for removing heavy metals and fine dust according to the present invention is prepared by adding 0.1 to 20.0 wt% of a mugwort extract to the total weight of the second composition (B) in order to further improve the heavy metal removal function.

The second composition (B) is composed of a mixture of 80.0 to 99.9 wt% of the second composition (B) and 0.1 to 20.0 wt% of the extract of Mugwort, more specifically, a mixture of 90.0 wt% of the second composition (B) and 10.0 wt% of the mugwort extract .

The extract is dried in a hot-air drier at 58 to 62 ° C for 10 to 14 hours, and the pulverized dried powder and distilled water are mixed at a weight ratio of 1:15 to 20, and the mixture is heated and extracted at 80 to 90 ° C for 1 to 3 hours. To obtain an extract, which is obtained by concentrating the extract at a reduced pressure of 0.1 to 0.3 mPA with a vacuum rotation evaporator for 5 to 7 hours.

More specifically,

The dried bean curds were dried at 60 ° C for 12 hours in a hot air drier. The dried bean curd powder and distilled water were mixed at a weight ratio of 1:18, and the mixture was heated at 85 ° C for 2 hours to obtain an extract. Which is obtained by concentration under reduced pressure using a vacuum rotation evaporator for 6 hours.

The above-mentioned Gelidium amansii is a red alga belonging to the genus Epidermidis, and is known to have functions such as antioxidation, immunity and anticancer, and anti-obesity. Extracts of mugwort are rich in flavonoids and polyphenols, and have various physiological effects.

The cosmetic composition for removing heavy metals and fine dust according to the present invention is prepared by further adding 0.1-20.0 wt% of a gangue extract to the total weight of the second composition (B) to further improve the heavy metal removal function.

The second composition (B) is composed of a mixture of 80.0 to 99.9 wt% of the second composition (B) and 0.1 to 20.0 wt% of the ginger extract, more specifically, a mixture of 90.0 wt% of the second composition (B) and 10.0 wt% of the ginger extract .

The gut extract was prepared by mixing guttae powder and methanol at a weight ratio of 1:10 to 15, and then extracting the mixture while stirring at room temperature for 22 to 26 hours, filtering the extract, filtering the filtrate at a concentration of 0.1 to 0.3 mPa, And then concentrated under reduced pressure using a vacuum rotation evaporator for 7 hours.

More specifically,

The mixture was mixed at a weight ratio of 1:12, and the mixture was extracted with stirring at room temperature for 25 hours. The extract was filtered, and the filtrate was vacuum-dried by vacuum rotation evaporator under the condition of 0.2 mPA for 6 hours. The product obtained by concentration is used.

The cosmetic composition for removing heavy metals and fine dust according to the present invention is prepared by further adding 0.1 to 20.0 wt% of an extract of anchovy wilt to the total weight of the second composition (B) in order to further improve the heavy metal removal function.

(B) and 0.1 to 20.0 wt.% Of an extract of anchovy. The present invention relates to a composition comprising 90.0 wt% of a second composition (B) and 10.0 wt% of an extract of anchovy Lt; / RTI &gt;

The cosmetic composition for removing heavy metals and fine dust according to the present invention is prepared by further adding 0.1 to 10.0 wt% of a third composition to the second composition in order to further extend the preservation period.

The third composition comprises a mixture of 0.01 to 50.0 wt% of an acidic extract, 0.01 to 50.0 wt% of a moss extract, 0.01 to 50.0 wt% of an extract of Baekduong, and 0.01 to 50.0 wt% of a grapefruit seed extract. 25.0 wt.% Of mungbean extract, 25.0 wt.% Of moss extract, 25.0 wt.% Of Baekduong extract and 25.0 wt.% Of grapefruit seed extract.

0.01 to 3.0 wt% of the third composition thus prepared is further added to the second composition.

More specifically, it is more specifically composed of a mixture of 99.0 wt% of the second composition (B) and 1.0 wt% of the third composition.

The third composition has a function of extending the preservation period of cosmetic compositions and cosmetics as a natural preservative composed of an acidulant extract, a moss extract, a Baekjong extract, and a grapefruit seed extract.

Zanthoxylum schinfolium (Zanthoxylum schinfolium) is a species native to the altitude less than 1,000m altitude in Korea and its fruit is used as vegetable oil resources and various seasonings, and seed oil is also used as a lubricant for millet.

Sancho is the fruit of the sancho (Japanese apricot) tree. The fruit skin contains 2 ~ 4% of essential oil. Its main ingredients are dipentene (dl-limonene), citronellal, l-β-phellandrene, geraniol, citronellol Respectively. The fruit contains xanthoxine, a phlegmatic toxin, xanthoxine acid, a paralytic component, sterol campesterol, stigmasterol, and β-sitosterol.

The above-mentioned extract of the sancho extracts was mixed with the mixture of methanol and methanol at a weight ratio of 1:10 to 15, and then extracted with stirring at room temperature for 22 to 26 hours. The extracted extract was filtered with a filter paper, Under reduced pressure for 5 to 7 hours using a vacuum rotation evaporator.

As a specific example of the above-mentioned sancho extract, 1,000 g of anthocyanin and 10 L of methanol are mixed and extracted with stirring at room temperature for 24 hours. Filter paper No. 2, and concentrated under reduced pressure using a vacuum rotation evaporator at 62 DEG C and 0.2 mPA for 5 hours to obtain 1 L of an extract.

Moss has been reported to have excellent antimicrobial and antioxidant effects. When mosses are added to water, the acidity of the surrounding water is increased by the object of mosses in the body, thereby reducing the bacterial activity and preventing the deterioration of water.

There are lichen (moss, moss, Musci), umbrella (liverwort, Hepaticae), and horny lichen (hornwort, Anthocerotae). These lichens inhibit microbial propagation and are highly valuable as natural preservatives because of their antioxidant action.

The moss extract was washed with hot water at 55 to 65 ° C and then dried by hot air blow. The moss was mixed with 100% methanol at a weight ratio of 1:10 to 15, The extract was filtered with a filter paper and the filtrate was concentrated under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA Is used.

As a specific example of the moss extract, a moss mixture obtained by mixing moss, large feather moss and feather moss at the same weight ratio is dewatered by water washing and then dried by hot air drying at 62 ° C for 6 hours. 1,000 g of the pulverized dried sample and 10 L of 100% methanol are mixed in a reflux condenser equipped with a cooling tube and reflux cooled for extraction at 58 ° C. for 7 hours.

The extract was filtered with a pore size filter paper having a pore size of 5 μm and concentrated under reduced pressure at 62 ° C. and 0.2 mPA using a vacuum rotation evaporator for 5 hours to obtain 1 L of an extract.

It is a perennial plant belonging to the ranunculaceae family. It is distributed in all parts of Korea and also in northeast China. This herbal medicine has been commonly applied to toothache, fever, and water, and has been used for anti-inflammation, astringency, hemostasis, dysentery, detoxification, scrotum, antibacterial, sedation, and anticancer. It has been reported that this material has been widely used for anti-inflammation, analgesic, antipyretic, and detoxification purposes in Korea.

The Baekhwong extract is washed with water, dried in a freeze dryer, processed into a powder form, powdered Baekjong and distilled water are mixed at a weight ratio of 1:10 to 12, And the extracted extract is filtered with a filter paper and the filtrate is concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.

As a specific example of the Baechuonggong extract, 1,000 g of Baekhwong in the form of powder is mixed with 10 L of distilled water to form a mixture, and the mixture is reflux-extracted at 80 ° C for 3 hours. Filter the extract with filter paper.

The filtered extract was concentrated under reduced pressure at 62 DEG C and 0.2 mPA for 5 hours using a vacuum rotation evaporator to obtain 1 L of an extract.

Grapefruit is juicy and has a bit of sour taste. Grapefruit seed extract is a natural antimicrobial material, and its main components are ascorbic acid, palmitic acid, glucoside, and naringin glycocortoferol, which inhibit mycotoxin biosynthesis and growth of fungal strains.

The grapefruit seed extract is prepared by mixing the grapefruit seed with ethanol at a weight ratio of 1: 5 to 10, and then refluxing cooled for extraction at a temperature of 60 to 62 ° C for 2 to 3 hours. The extracted extract is filtered with filter paper The filtrate is centrifuged at 10,000 × g for 20 to 40 minutes, and the supernatant obtained by concentrating the supernatant under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA with a vacuum rotation evaporator is used.

As a specific example, grapefruit seed and ethanol were mixed at a weight ratio of 1: 8, and the mixture was refluxed and cooled for 2 hours at a temperature of 60 ° C. The extracted extract was filtered with a filter paper, For 30 minutes, and the supernatant obtained by concentrating the supernatant under reduced pressure for 5 hours under a condition of 0.2 mPA with a vacuum rotation evaporator.

1. Add 703.15 g DI Water, 2.0 g Carbopol, 50.0 g Glycerine + 0.05 g Xanthan gum, 2.0 g Allantoin, 5.0 g Betaine, , 1.0 g of arginine, 30.0 g of trehalose and 0.4 g of adenosine are mixed to prepare an aqueous phase.

5.0 g of Lipoid S75-3, 5.0 g of glycerol monostearate-105 (GMS 105), 5.0 g of Bees Wax, 10.0 g of Cetearyl Alcohol, 10.0 g of Shea butter g, 10.0 g of phenoxyethanol, 50.0 g of caprylic / capric triglyceride (MCT), 15.0 g of Tween 60, 15.0 g of Grape Seed Oil, Oil (25.0 g) and methyl parahydroxy benzoate (1.0 g) are melted by heating to make an oil phase.

3. The oil phase is mixed with the water phase to form a mixture.

4. When the temperature of the mixture falls below 38 ° C, 50 g of the cosmetic composition of Example 3, 5.0 g of cyclomethicone (DC345) and 0.4 g of perfume are added and stirred to complete the lotion.

In Comparative Example 1, the amount of purified water (D.I Water) was 753.15 g, and the lotion was completed without using the cosmetic composition according to the present invention.

The lotion is prepared in the same manner as in Example 9 except that 50.0 g of the cosmetic composition of Example 4 is used in the case of using the cosmetic composition.

The lotion is prepared in the same manner as in Example 9 except that 50.0 g of the cosmetic composition of Example 5 is used in the case of using the cosmetic composition.

The lotion is prepared in the same manner as in Example 9 except that 50.0 g of the cosmetic composition of Example 6 is used in the case of using the cosmetic composition.

The lotion is prepared in the same manner as in Example 9 except that 50.0 g of the cosmetic composition of Example 7 is used in the case of using the cosmetic composition.

The lotion is manufactured in the same manner as in Example 9 except that 50.0 g of the cosmetic composition of Example 8 is used in the case of using the cosmetic composition.

[Test Example 1]

<Antioxidant measurement>

The antioxidative effects of the lotions according to Examples 9 to 14 are measured.

After diluting the lotion samples of Examples 9 to 14 and Comparative Example 1 with 50% dimethyl sulfoxide (DMSO), 80 쨉 l of each lotion sample was taken, and 10 mM DPPH (1,1-diphenyl-2 -picrylhydrazyl) solution was added thereto, followed by vortexing for 15 seconds. Thereafter, 1 mL of distilled water was added, and the mixture was allowed to stand at 40 DEG C for 35 minutes and absorbance was measured at 517 nm as compared with Comparative Example 1. [

As a result of measuring the antioxidative activity at 40 캜,

51.89% in Example 9, 52.41% in Example 10, 53.01% in Example 11, 56.87% in Example 12, 57.54% in Example 13, and 53.11% in Example 14. Comparative Example 1 was measured to be 11.29%.

The antioxidative effects of the cosmetics according to Examples 9 to 14 were confirmed in the order of Example 13> Example 12> Example 14> Example 11> Example 10> Example 9. It can be confirmed that Examples 9 to 14 have higher antioxidative activity than Comparative Example 1 although they show a slight difference in antioxidative activity depending on the formulation.

[Test Example 2]

<Antimicrobial measurement>

The antibacterial effects of the lotions of Examples 9 to 14 are to be measured.

Antimicrobial tests were performed on the strains Staphylococcus aureus KCCM 11335, Escherichia coli, Pseudomonas aeruginosa KCCM 11802, Candida albicans KCCM 11282 and Aspagillusniger KCCM 60143.

E. coli, KCCM 40271), Candida albicans (C. albicans KCCM 11282), fungi (E. coli KCCM 11282), and yeast strains of Staphylococcus aureus (S. aureus, KCCM 11335), Pseudomonas aeroginosa (P. aeroginosa, KCCM 11802) and Escherichia coli Cultured Aspergillus niger (A. niger KCCM 60143) was cultured in a shaking incubator at 37 ° C and fungi at 26 ° C and 250 rpm.

The bacterial strains thus cultivated were inoculated into the cosmetics of Examples 9 to 14, and the viable cell counts were measured on days 1, 3 and 7, respectively. As a result of measurement, in each of Examples 9 to 14, Was not detected.

[Test Example 3]

&Lt; Measurement of wrinkle improvement effect &

1. Elastase inhibition rate measurement

Elastase and N-succinyl- (Ala) 3-p-nitroanilide increased the activity. The degree of inhibition by 50% activity was measured by IC ₅₀ (㎍ / ml).

20 μl of the sample solution in which each of the cosmetic compositions of Examples 3 to 8 was diluted to 500 μg / ml and 10 μl of 2.5 U Elastase were added to 200 μl of the substrate 1.0 mM N-succinyl- (Ala) 3-p-nitroanilide After incubation at 25 ° C for 10 min, absorbance was measured at 410 nm with an ELISA reader. Elastase inhibition rate is expressed as the absorbance reduction rate of the addition of the sample solution and the non-addition solution as follows.

Inhibition rate (%) = {1- (absorbance of sample added / absorbance of non-additive)} x 100

The inhibition rate of Example 3 was 54.78%, the inhibition rate of Example 4 was 57.11%, the inhibition rate of Example 5 was 52.58%, the inhibition rate of Example 6 was 52.58% 56.71%, the inhibition rate of Example 7 was 54.40%, and the inhibition rate of Example 8 was 58.01%.

2. Measurement of collagenase inhibition rate

The degree of inhibition was measured by IC ₅₀ (㎍ / ㎖) at 50% reduction in activity by evaluating the activity of collagenase degrading collagen.

Collagen is dissolved in 20 mM Tris-HCl (pH 7.5) and 5 mM CaCl ₂ buffer solution to make 3 μg / ml of substrate solution. 200 μl of Buffer, 20 μl of the substrate solution, 20 μl of the sample solution diluted to 500 μg / ml of each of the cosmetic compositions of Examples 3 to 8 was added, and the mixture was reacted at 37 ° C. for 1 hour with addition of collagenase. 100 μl of 2% ninhydrin reagent was added and incubated for 20 minutes. Absorbance was measured at 570 nm using an ELISA reader.

As a result of measurement, the inhibition rate in Example 3 was 80.51%, the inhibition rate in Example 4 was 79.11%, the inhibition rate in Example 5 was 79.87%, the inhibition rate in Example 6 was 78.95%, the inhibition rate in Example 7 was 79.87% Was inhibited by 75.98%.

The results of the Elastase inhibition rate and Collagenase inhibition rate of the cosmetic compositions of Examples 3 to 8 confirmed that wrinkles were improved, and the effect of improving the wrinkles of the cosmetic compositions containing the cosmetic compositions could be predicted.

[Test Example 4]

<Measurement of whitening effect>

The melanocyte present in the basal layer of the skin protects the skin by synthesizing melanin from tyrosine, which is stimulated by ultraviolet rays. However, excessive production of melanin can cause aesthetic and skin health problems, thus protecting the skin from ultraviolet rays or inhibiting the production of melanin.

In order to examine the whitening efficacy of cosmetics, twelve women aged 20 to 40 years without skin disease were selected as subjects to be tested for inhibition of tyrosinase activity and inhibition of melanin production.

1. Measurement of inhibition rate of tyrosinase activity

Tyrosinase is the only enzyme involved in the biosynthesis of melanin, the pigment component of the skin. The formula for the inhibition of tyrosinase activity is as follows.

Inhibition rate of tyrosinase activity (%)

= 100 - [(b-b ') / (a-a')] 100

a: Absorbance after reaction of blank sample

b: Absorbance after reaction of the sample solution

a ', b': absorbance measured by replacing with tyrosinase buffer

0.5 ml of 0.02 M sodium phosphate buffer (pH 6.5), 0.2 ml of 10 mM L-DOPA substrate and 0.1 ml of the cosmetic composition selected from Examples 3 to 8 were separately added to a 96-well plate, followed by addition of mushroom tyrosinase 110 Unit / mL) was added and reacted at 37 ℃ for 2 minutes. Absorbance was measured at 475 ㎚.

As a comparative example, albutin was used as a control group.

In order to confirm the inhibitory effect of tyrosinase, albutin (comparative example) used in cosmetics as a whitening agent in place of the cosmetic composition according to Examples 3 to 8 (comparative example) and in Examples 3 to 8 The whitening activity of the cosmetic composition according to the present invention was compared.

The whitening activity of 0.1% albutin in the comparative example was measured to be 68.53%.

The whitening activity of Example 3 was 65.61%; The whitening activity of Example 4 was 63.79%; The whitening activity of Example 5 was 60.24%; The whitening activity of Example 6 was 61.14%; The whitening activity of Example 7 was 65.58%; The whitening activity of Example 8 was 61.90%; showing a high activity almost similar to that of albutin used in medicines and cosmetics as a whitening agent.

Accordingly, it can be seen that the whitening effect of the cosmetic composition according to Examples 3 to 8 according to the present invention is large.

2. Melanin production inhibition

B16 melanoma cells were plated at 3 ㎖ in 6 well plates and cultured in phenol red-free DMEM solution containing 10% FBS for 12 hours.

Each of the cosmetic compositions according to Examples 3 to 8 was pre-treated at 36 ° C for 12 minutes, treated with 1 μM MSH, and cultured at 37 ° C for 48 hours.

After incubation, add 100 μL of 10 mM sodium phosphate buffer (pH 6.8) containing 1% (w / v) Triton X-100, shake for 5 minutes, and transfer to an eppendorf tube.

The cell pellet obtained by centrifugation at 3,000 rpm for 5 minutes was added with 100 μL of 1 N NaOH and 200 μL of distilled water. The melanin was completely dissolved by incubation at 60 ° C. for 1 hour. Then, 200 μL was transferred to a 96-well plate and absorbance was measured at 405 nm Respectively.

The absorbance at 490 nm wavelength was measured and compared using a Microplate Reader (Infinite 200, Tecan Group Ltd., Switzerland).

Inhibition (%) = [1- (A1 / A0)] 100

A0: Absorbance of non-additive (control)

A1: Absorbance of sample added sphere

As a result, the inhibitory activity of Example 3 was 65.57%; The inhibitory power of Example 4 was 66.12%; The inhibitory potency of Example 5 was 66.02%; The inhibition of Example 6 was 65.27%; The inhibition of Example 7 was 64.75%; The inhibitory activity of Example 8 was 63.12%; thus, it was confirmed that the inhibitory activity was excellent.

As a result of the tyrosinase activity inhibition result and the inhibition of melanin formation, it can be expected that the cosmetic composition according to the present invention will have a high whitening effect when applied to cosmetics.

[Test Example 5]

<Anti-Aging Measurement>

The anti-aging effects of the cosmetic composition according to Examples 3 to 8 will be measured.

Skin aging caused by photoaging during skin aging is caused by irregular pigmentation, skin sagging, vasodilation, reduced elasticity, blistering and deep and thick wrinkles caused by exposure to ultraviolet rays, and the skin becomes very rough and dry. In particular, the amount of collagen in the skin is reduced and the elastic fibers are denatured, resulting in wrinkles.

Due to the action of ultraviolet rays, the skin over-produces harmful reactive oxygen species (ROS) including superoxide anion, peroxide, singlet oxygen and the like, Low molecular weight antioxidants such as enzymes and glutathione, vitamin E, vitamin C and ubiquinol are reduced. Oxidative stress induces lipid peroxidation, protein oxidation, activation of proteolytic enzymes that destroy epilepsy, destruction of elastic fibers such as collagen and elastin, damage of cellular components, and promotes photoaging.

The reactive oxygen species (ROS) is an intermediate product produced when oxygen molecules are incompletely reduced during the cell respiration process, and refers to oxygen having a free radical. ROS occurs naturally by the normal metabolism of oxygen and plays an important role in cell signaling and homeostasis. The concentration of ROS can dramatically increase due to environmental stresses such as exposure to ultraviolet radiation or high heat.

The increase of ROS causes oxidative stress, which causes skin diseases such as photoaging and pigmentation.

In order to investigate the effect of the cosmetic composition according to Examples 3 to 8 on the photoaging effect, experiments were carried out using HaCaT cell (ATCC, Manassas, VA, USA) which is a human keratinocyte cell line.

HaCaT cells were cultured in a medium supplemented with normal medium (Comparative Example 1) or 100 μg / ml of the cosmetic composition according to Examples 3 to 8 for 24 hours, irradiated with UVB 80 mJ / cm 2, and then measured with a Multi-Mode Microplate Reader Respectively.

Compared with Comparative Example 1 in which HaCaT cells were irradiated with UVB 80 mJ / cm 2,

When treated with the cosmetic composition according to Example 3, the ROS was 76.7%

When treated with the cosmetic composition according to Example 4, the ROS was 76.9%

When treated with the cosmetic composition according to Example 5, the ROS was 78.5%

When treated with the cosmetic composition according to Example 6, the ROS was 79.1%

When treated with the cosmetic composition according to Example 7, the ROS was 78.1%

When treated with the cosmetic composition according to Example 8, the ROS decreased to 79.2%. From these results, it was confirmed that the cosmetic composition according to the present invention has an inhibitory effect on active oxygen, and the anti-aging effect of the cosmetic product can be predicted.

[Test Example 6]

The effect of removing the heavy metals and fine dust of the lotion prepared according to Examples 9 to 14 will be measured.

<Removal effect of heavy metal and fine dust>

In order to confirm the fine dust removing effect by the lotion prepared according to Examples 9 to 14, the heavy metal and fine dust adsorption ratios of the lotion were measured.

Atmospheric heavy metal is a metal having a specific gravity of about 4.0 to 5.0 or more, and examples thereof include cadmium (Cd), lead (Pb), chromium (Cr), copper (Cu), and manganese (Mn).

The adsorption rate was measured 10 times by atomic absorption spectrometry after reacting the lotion with cadmium (Cd) (30 ppm), lead (Pb) (20 ppm) and fine dust (100 ppm) As a result, the average adsorption rates (%) of Examples 9 to 14 were measured as 79% of cadmium (Cd), 75% of lead (Pb) and 89% of fine dust.

Thus, it was confirmed that the lotion prepared according to Examples 9 to 14 had an excellent effect of removing heavy metals and fine dusts.

The present invention provides a cosmetic composition and a cosmetic composition for removing heavy metals and fine dusts in accordance with an increase in air pollution caused by fine dust which is getting worse recently, thereby minimizing the amount of fine dust penetrating through the skin, The safety of use by the user can be ensured, which can increase the satisfaction of the consumer and thus the possibility of industrial use is great.

Claims (7)

The mixture of sea cucumber and ethanol was mixed at a weight ratio of 1:10 to 12, and the mixture was extracted with shaking incubator at 120 to 160 rpm at 24 to 26 ° C for 65 to 75 hours. The extracted extract was centrifuged at 7,500 to 8,500 rpm for 15 to 25 Min. The supernatant obtained by centrifuging was filtered with a filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPa by a vacuum rotation evaporator.
The mixture of gold and methanol was mixed at a weight ratio of 1: 4 to 6, and the mixture was refluxed at 65 to 75 ° C for 20 to 30 hours. The extracted extract was centrifuged at 3,500 to 4,500 rpm for 4 to 6 minutes to obtain a supernatant The mixture was filtered with a filter paper, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under a condition of 0.1 to 0.3 mPA with a vacuum rotation evaporator to obtain a first mixture composition a1) 0.5 to 60.0 wt%;
Flaxseed powder and ethanol are mixed at a weight ratio of 1:10 to 15, and then the mixture is dipped at 50 to 80 ° C for 2 to 10 hours to extract. The extracted extract is filtered with a filter paper, For 5 to 7 hours under reduced pressure using a vacuum rotation evaporator,
Vitamin tree leaves and ethanol were mixed at a weight ratio of 1:15 to 25 and then dipped at room temperature for 22 to 26 hours to separate the supernatant and the precipitate. The same extract was repeated three times to filter paper 0.5 to 60.0 wt% of a second mixed composition (a2) prepared by mixing and filtering the filtrate, and concentrating the filtrate at reduced pressure with a vacuum rotation evaporator at 58 to 63 ° C for 5 to 7 hours, ;
The mixture of black garlic and distilled water was mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken for 1 to 5 hours at 100 DEG C and 100 to 140 rpm. The extracted extract was filtered with a filter paper, The extract of black garlic obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of ~ 0.3 mPA,
The motherwort and distilled water are mixed at a weight ratio of 1: 5 to 30, and the mixture is shaken for 1 to 5 hours at 100 ° C and 100 to 140 rpm. The extracted extract is centrifuged at 2,000 to 4,000 rpm for 10 to 30 minutes After centrifuging, the supernatant was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA, (A3) 0.5 to 60.0 wt%;
The mixture of persimmon leaves and ethanol was mixed at a weight ratio of 1: 5 ~ 30, and the mixture was reflux cooled for 2 ~ 6 hours at 42 ~ 48 ℃. The extract was centrifuged at 11,000 ~ 13,000 rpm for 20 ~ The supernatant was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA.
The ration flowers and 70% ethanol were mixed at a weight ratio of 1: 5 to 30, and the mixture was shaken at 23 to 28 ° C and 80 to 150 rpm for 40 to 55 hours. The obtained extract was filtered with a filter paper 0.5 to 60.0 wt% of a fourth mixed composition (a4) prepared by concentrating the filtrate under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA in the same weight ratio;
Chestnut leaf and ethanol were mixed at a weight ratio of 1:10 to 15, and then the mixture was immersed at room temperature for 22 to 26 hours to separate the supernatant and the precipitate. The same procedure was repeated three times, and the extracted extract was filtered with a filter paper , Chestnut leaf extract obtained by concentrating the filtrate under reduced pressure at 58 to 63 ° C for 5 to 7 hours with a vacuum rotation evaporator,
After drying and finishing the Acacia (Robiniapseudoacacia) flower, the powder is mixed with the purified water at a weight ratio of 1:35 to 45, and then extracted at 85 to 99 ° C for 5 to 7 hours. The extracted extract is filtered with filter paper , And the filtrate was centrifuged at 2,000 ~ 4,000 rpm for 10 ~ 30 minutes in a centrifuge. The supernatant was filtered and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA (A1 + a2 + a3 + a4 + a5) of 0.5 to 60.0 wt% of a fifth mixed composition (a5) prepared by mixing the obtained acacia flower extract in a weight ratio of 100.0 wt% ) 50.0 to 90.0 wt%
The mixture of soil and ethanol was mixed at a weight ratio of 1: 5 ~ 30, and the mixture was immersed at 50 ~ 80 ℃ for 2 ~ 10 hours. The extracted extract was filtered with a filter paper to obtain a filtrate of 0.1 ~ 0.3 mPA 20.0 to 80.0 wt% of the extract obtained by concentrating the extract under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the conditions;
Seaweed mixed with seaweed, kelp, rhubarb, and mackerel were mixed at a weight ratio of 58 to 62 ° C for 10 to 14 hours in a hot air drier, and the pulverized dried powder and distilled water were mixed at a weight ratio of 1:15 to 20, The resulting extract was filtered with a filter paper and concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPa 5.0 to 30.0 wt% of a second composition (B) of 100.0 wt% composed of a mixture of 20.0 to 80.0 wt% of the obtained seaweed extract,
The chrysanthemum is dried and powdered. Chrysanthemum powder and distilled water are mixed at a weight ratio of 1:10 to 12 times, refluxed at 75 to 85 ° C for 2 to 4 hours, (C) 5.0-20.0 wt% obtained by filtration with filter paper and concentrating the filtrate under reduced pressure for 5 to 7 hours under a condition of 0.1-0.3 mPA with a vacuum rotation evaporator. And a cosmetic composition for removing heavy metals.
The method according to claim 1,
The second composition (B)
The mugwort was dried in a hot air drier at 58 to 62 ° C for 10 to 14 hours, and the pulverized dried powder and distilled water were mixed at a weight ratio of 1:15 to 20 and heated at 80 to 90 ° C for 1 to 3 hours to obtain an extract , And 0.1 to 20.0 wt% of a mugwort extract obtained by concentrating the extract with a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours under reduced pressure. A cosmetic composition for removing heavy metals and fine dusts.
The method according to claim 1,
The second composition (B)
The dried beans were dried at 58 to 62 ° C for 10 to 14 hours in a hot air drier, and then the dried powder and the distilled water were mixed at a weight ratio of 1:15 to 20 by weight. The mixture was heated and extracted at 80 to 90 ° C for 1 to 3 hours to obtain an extract , And 0.1 to 20.0 wt% of an extract of Mugwort extract obtained by concentrating the extract with a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours under reduced pressure. A cosmetic composition for removing heavy metals and fine dusts.
The method according to claim 1,
The second composition (B)
The mixture was mixed at a weight ratio of 1:10 to 15, and the mixture was extracted with stirring at room temperature for 22 to 26 hours. The extract was filtered, and the filtrate was concentrated under reduced pressure for 5 to 7 hours under the condition of 0.1 to 0.3 mPa And 0.1 to 20.0 wt% of a ginger extract obtained by concentration under reduced pressure with a vacuum rotation evaporator. The cosmetic composition for removing heavy metals and fine dust according to claim 1,
The method according to claim 1,
The second composition (B)
The dried seaweeds were dried at 58 to 62 ° C for 10 to 14 hours, mixed with dried powder and distilled water at a weight ratio of 1:15 to 20, and heated at 80 to 90 ° C for 1 to 3 hours. , And 0.1 to 20.0 wt% of an extract from anchovy safflower extract obtained by concentrating the extract at a reduced pressure with a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours. A cosmetic composition for removing heavy metals and fine dusts containing an extract.
The method according to claim 1,
The second composition (B)
The first composition (A) is prepared by mixing the mixture of sancho and methanol in a weight ratio of 1:10 to 15, then extracting the mixture at room temperature with stirring for 22 to 26 hours, filtering the extracted extract with a filter paper, 0.01 to 50.0 wt% of the acantholyticum extract obtained by concentration under reduced pressure with a vacuum rotation evaporator for 5 to 7 hours under the condition of ~ 0.3 mPA;
The moss was washed with hot air at 55 to 65 ° C and then pulverized. The pulverized moss and 100% methanol were mixed at a weight ratio of 1:10 to 15, and the mixture was cooled to a temperature of 58 to 62 ° C at a temperature of 58 to 62 ° C The extract was filtered with a filter paper, and the filtrate was concentrated under reduced pressure using a vacuum rotation evaporator for 5 to 7 hours under the condition of 0.1 to 0.3 mPA. 0.01 to 50.0 wt% of extract;
Dried in a freeze drier, processed into powder form, powdered Baekjong and distilled water were mixed at a weight ratio of 1:10 to 12 times, and the mixture was refluxed at 75 to 85 ° C for 2 to 4 hours 0.01 to 50.0 wt% of Baechutong extract obtained by vacuum filtration of the filtrate with a vacuum rotation evaporator under the condition of 0.1 to 0.3 mPA for 5 to 7 hours, and extracting the extracted extract with filter paper.
The mixture of grapefruit seed and ethanol is mixed at a weight ratio of 1: 5 to 10, and the mixture is refluxed and cooled at a temperature of 60 to 62 ° C for 2 to 3 hours. The extracted extract is filtered with a filter paper, × g for 20 to 40 minutes, and the obtained supernatant was concentrated with a vacuum rotation evaporator under a reduced pressure of 0.1 to 0.3 mPA for 5 to 7 hours to obtain a mixture of a grapefruit seed extract of 0.01 to 50.0 wt% Wherein the composition further comprises 0.01 to 3.0 wt% of a third composition of 100.0 wt% of the composition.
The cosmetic composition according to any one of claims 1 to 6, which is prepared by adding 0.1 to 50.0 wt% of any one cosmetic composition selected from the group consisting of one of claims 1 to 6 so as to be included in the total weight of the cosmetic.