KR20210115738A - Cosmetic Composition for Protecting Skin against Particulate Matter - Google Patents

Abstract

The present invention relates to an extract obtained by extracting sea swallow's nest, Brasenia schreberi, Saccharina japonica, Ecklonia cava, and grapes by low-temperature aging and/or ultrasonic extraction. The extract of the present invention is rich in polysaccharide mucus components, which can effectively prevent the penetration of fine dust into the skin, and has antioxidant components such as polyphenols to relieve irritation caused by fine dust and alleviate or prevent inflammation. Furthermore, the extract of the present invention has an excellent effect in maintaining the elasticity of the skin and alleviating wrinkles.

Description

Cosmetic Composition for Protecting Skin against Particulate Matter}

The present invention relates to a cosmetic composition for skin protection against fine dust, a quasi-drug composition, and a composition for external use.

Particulate matter floating in the air is called dust, and among them, particles with a size that cannot be identified with the naked eye are called fine dust. Fine dust is classified by particle size, and is named PM 10, PM 2.5, and PM 1 according to the size, which means the particle size unit of particles in the atmosphere. PM is an abbreviation of particular matter, and PM 10 refers to fine dust that we commonly know as particles with a particle size of 10 μm or less. PM 2.5 is a size called ultra-fine dust or ultra-fine dust and has a particle diameter of 2.5 μm or less. Particles with a size of 1 μm or less are also referred to as PM 1 . The reason for classifying the particle size range from PM 10 to PM 1 is that the smaller the particle size, the greater the impact on the human body and the environment and the more harmful.

Ultrafine dust containing various chemicals such as heavy metals such as lead and cadmium weakens the metabolic flow of the skin, lowering the function of controlling sebum, causing dryness, itchiness, and skin troubles. Heavy metals and environmental hormones contained in fine dust Harmful substances such as other harmful substances promote the secretion of inflammatory cytokines in cells and increase the number of white blood cells, causing an allergic reaction to atopic dermatitis. In addition, when fine dust is absorbed into the skin, it stimulates pigment cells to create wrinkles and age spots. When fine dust is absorbed into the body, it creates a toxic substance called active oxygen, which damages collagen in the skin, reducing the elasticity of the skin, and as a result, causes extrinsic aging. In particular, when fine dust is in direct contact with the skin, which is located at the outermost part of our body, the skin acts as a barrier between the organism and the environment. causes several problems. In particular, fine dust is 20 times smaller than pores, so it can easily penetrate the skin of the human body. For example, the skin, which is the main site for absorbing substances for environmental pollution, is regarded as the same as inhalation through the respiratory system for substances such as chloroform, which is a toxic substance, to be absorbed into the skin.

Accordingly, there is a need for research on cosmetics and external preparations to improve and protect skin irritation caused by fine dust. Republic of Korea Patent No. 10-1850313 (Registration Date 2018.04.13) 'Cosmetic composition for removing heavy metals or fine dust containing chrysanthemum extract as an active ingredient'; Korean Patent Laid-Open Patent Publication No. 10-2018-0020574 (published on February 28, 2018) 'Cosmetic composition for removing heavy metals or fine dust containing Gwaneumjuk and Indian rubber tree extract as active ingredients'; Korean Patent Application Laid-Open No. 10-2018-0020573 (published on February 28, 2018) 'Cosmetic composition for removing heavy metals or fine dust containing Panda rubber tree and Skindapsus extract as active ingredients'; Korean Patent Laid-Open Patent No. 10-2018-0020571 (published on February 28, 2018) 'Cosmetic composition for removing heavy metals or fine dust containing an extract of Indian Bodhi tree as an active ingredient'; Republic of Korea Patent No. 10-1643696 (Registration date: July 22, 2016) 'Chemical composition for removing heavy metals and fine dust containing leeks'; Republic of Korea Patent No. 10-1703928 (Registration Date 2017.02.01) 'Cosmetic composition for removing fine dust and heavy metals'; Republic of Korea Patent No. 10-1786861 (registration date 2017.10.11) has disclosed a technology for 'a cosmetic composition for removing heavy metals and fine dust'.

However, up to now, there has not been developed a herbal medicine-based cosmetic that directly protects the skin surface to prevent the penetration of fine dust while at the same time improving irritation caused by fine dust, alleviating inflammation, and soothing the skin. In this situation, the present inventors developed the extract containing mucopolysaccharide and antioxidant as an active ingredient of a skin protection cosmetic as a result of applying the optimal extraction method to the mixed herbal medicines, and completed the present invention as a result of confirming its efficacy.

The technical problem to be achieved by the present invention is a herbal medicine comprising 1.0 to 2.0 parts by weight of bird's nest in a solvent, 0.5 to 1.5 parts by weight of pure vegetables, 1.0 to 2.0 parts by weight of kelp, 0.5 to 1.5 parts by weight of Ecklonia cava, and 0.5 to 1.5 parts by weight of grapes It is to provide a cosmetic composition for skin protection comprising an extract extracted from the immersed mixture as an active ingredient.

In addition, another technical problem to be achieved by the present invention is to provide a quasi-drug composition for skin protection comprising the extract as an active ingredient.

In addition, another technical problem to be achieved by the present invention is to provide a composition for external application for skin protection, comprising the extract as an active ingredient.

On the other hand, another technical problem to be achieved by the present invention is 1.0 to 2.0 parts by weight of bird's nest in a solvent, 0.5 to 1.5 parts by weight of pure vegetables, 1.0 to 2.0 parts by weight of kelp, 0.5 to 1.5 parts by weight of Ecklonia cava, and 0.5 to 1.5 parts by weight of grapes A first step of immersing the herbal material containing; It is to provide a method for producing a herbal extract for skin protection, comprising a second step of extracting the extract from the immersed mixture.

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those of ordinary skill in the art to which the present invention belongs from the description below. There will be.

As one aspect for solving the above problems, the present invention is 1.0 to 2.0 parts by weight of seabird's nest in a solvent, 0.5 to 1.5 parts by weight of pure vegetables, 1.0 to 2.0 parts by weight of kelp, 0.5 to 1.5 parts by weight of Ecklonia cava, and 0.5 to 0.5 parts by weight of grapes It provides a cosmetic composition for skin protection comprising, as an active ingredient, an extract extracted from a mixture in which herbal medicines containing 1.5 parts by weight are immersed.

The cosmetic composition for skin protection of the present invention contains, as an active ingredient, an extract extracted by mixing herbal materials described below in a predetermined ratio. Unless otherwise specified, herbal medicine refers to a cut or a processed product of the corresponding part of the plant described herein, unless otherwise specified, and the described plant is mainly expressed by scientific name, but includes varieties and subspecies.

Swallow's nest

The sea Swallow, also called the Golden Swallow or Golden Swallow, is a dark brown bird with a body length of 12 to 17 cm. Mainly distributed in East Asia, the Himalayas, and Australia (northern). The sea swallow nest is a nest of sea swallows. It is made of small fish or sea horses after applying saliva to a rock crevice or cave wall on the coast. It has a yellowish color and becomes transparent over time. In Chinese cuisine, this nest is called “Yanwu” and is eaten. Unlike the swallowtail, it collects seaweed and fish bones and mixes saliva from the mouth to make a nest, so it is rich in nutrition and colloidal protein and is known to have a great tonic effect. It is introduced in 本草綱目拾遺 (本草綱目拾遺) that "makes the skin supple and it is beneficial to the blood and gives vitality".

It has been reported that swallow's nest contains a large amount of protein, amino acids, sugars, minerals, and antioxidants, and has effects such as promoting cell regeneration and enhancing immunity. The main component of swallow's nest is mucin, a substance secreted by the salivary glands. Swallow's nest is the result of the mucus coming out of the golden swallow's mouth that turns seaweed into gelatin, which is then collected and hardened into a nest. When the nest is soaked in water, it expands to about 20 times the volume and has the texture of gelatin.

The extract according to the present invention can exhibit the effect of making dry skin softer by using sea swallow's nest as a herbal medicine, and provides skin nutrition by providing vitamins C and E, arbutin, and collagen. In addition, it enhances natural healing power and regulates immunity. It contains proteins that nourish the skin, and other polyphenols, β-carotene, amino acids, and chondroitin components that help health and beauty. Bird's nest is also rich in amino acids, sulfur, and minerals, which can nourish the skin and improve elasticity. Since it contains a large amount of epidermal growth factor (EGF), it is also effective in promoting regeneration.

Sunchae (Brasenia schreberi)

Sunchae is a perennial aquatic plant of the Heterinaceae family, and its habitat is extremely limited. It is found in ponds rich in organic matter or in weakly acidic lakes, and is also called Bugyu or Sunnamul. It grows in ponds, but in the past, it was also cultivated in paddy fields to eat leaves and shoots. The rhizome grows to the side and grows to a length of 50 to 100 cm, and the leaf floats on the surface of the water. The leaves are alternate phyllotaxis and oval, the back side is purple, and the petiole hangs in the center. When the leaves grow, they are surrounded by a slime like agar along with the young stem.

Flowers bloom in May-August, are black, reddish purple, and hang one by one at the end of a long peduncle that grows from the leaf axil, and the diameter is about 2cm. There are three sepals and three petals each. There are 6-18 pistils, there are nipple-shaped projections, and there are many stamens. The stamen surrounds the pistil and is longer than the pistil, and the central pistil is curved outward. After the operation withers, the pistil grows and a sticky slime is formed on the head.

In the extract of the present invention, leaves, stems, or roots of pure vegetables may be used, and preferably, leaves, stems, or both may be used. The mucous polysaccharides surrounding the green vegetable stem are D-galactose (34.1%), D-glucuronic acid (17.3%), D-mannose (13.4%), L-rhamnose (11.4%), L-fucose (10.9%), D It consists of -xylose (7.0%) and L-arabinose (5.9%). The water content of this mucus is about 98%, and many studies have been reported on its activity. The vegetable mucopolysaccharide has excellent moisturizing effect on the skin and helps to improve skin texture.

It has been reported that pure mucinous polysaccharide can reduce skin roughness when applied to the skin of women in their twenties. In addition, it induces the formation of keratinocytes in keratinocytes to protect the skin from the external environment and reduce the amount of transdermal moisture loss, increases the expression of TGM1 gene, which is related to maintaining and moisturizing the skin barrier, and synthesizes hyaluronic acid, one of the skin moisturizing factors. It can increase the expression of genes AQP3 and HAS3 that promote In addition, the mucous polysaccharide of pure vegetables reduces NO production and promotes collagen synthesis.

Kelp( Saccharina japonica )

Kelp is a 2-3-year-old seaweed belonging to the brown algae plant. It is called Gonpo or Haetae. The leaves are yellowish-brown, band-shaped, 25-40 cm wide and 1.5-3.5 m long, and the stems are short, columnar, and stand upright. clings to the rock It is an annual plant distributed in the coasts of Handa and boreal zones. It is an algae composed mostly of carbohydrates and minerals, and is the most nutrient-rich plant on earth. Although there are some differences depending on the type of kelp, it generally contains about 16% water, 7% protein, 1.5% fat, 49% carbohydrate, and 26.5% minerals. 5~10% of carbohydrates are fiber, and the rest are polysaccharides such as laminarin and fucoidin, mainly alginic acid. In Donguibogam, it is said, “It softens hard things by controlling acidity, alleviating boils, and controlling lumps.”

For the extract of the present invention, leaves, stems, or roots of kelp may be used, and preferably, stems or whole parts may be used. Kelp is rich in polysaccharide components such as fucoidan, alginic acid, and laminaran. Fucoidan is a sulphated polysaccharide with a sticky, viscous structure and exhibits immune cell modulating action, anti-allergic action, and antiviral action. Alginic acid is a polysaccharide constituting the cell membrane of brown algae, and sodium alginate is used to increase the viscosity. Alginic acid is composed of D-mammuronic acid and Gulcuronic acid monomer. Laminaran (Laminaran) is a storage polysaccharide biosynthesized from brown algae and has anti-coagulant, anti-tumor, anti-viral, and anti-aging effects. As dietary fiber, these water-soluble polysaccharides have effects such as correcting action, excretion of heavy metals, and prevention of blood vessel deposition of cholesterol, anti-aging, and the like. In addition, the kelp extract has the effect of restoring the mitochondria of cells damaged by UV rays and protecting the cells from free radicals in the cells. Furthermore, it can exhibit whitening efficacy by inhibiting tyrosinase activity and inhibiting melanin synthesis.

Ecklonia ( Eckloina cava )

Gamtae is an edible seaweed and is a plant of the brown algae family, Laminariales, and Alariaceae. The length is 1~2m, the stem is cylindrical, and the base is root-shaped. The middle part is thick, and it is full when young, but it is often hollow when grown up. At the end of the stem, there is one flat central leaf with side leaf pieces. This leaf is about 1m long and brown, but turns black when dried. It is mainly fed to abalone and conch, and is used as a main raw material for making alginic acid or potassium iodide.

Recently, as various physiological activities have been revealed through active research on marine biological resources, its potential as a functional material is being recognized. According to several reports, it is known that Ecklonia cava extract has radical scavenging, antioxidant, and antibacterial effects and has a strong inhibitory effect on matrix metalloproteinases (MMPs).

In the extract of the present invention, leaves, stems, roots, or both of Ecklonia cava may be used as raw materials, and preferably leaves, stems, or both of them may be used. Ecklonia cava is rich in polyphenolic compounds of phlorotannin, whose basic composition is phloroglucinol. Although phlorotannin has been found in various brown algae, the existence of phlorotannin has been reported separately from Ecklonia cava and Gompi in Korea. Phlorotannin, a polyphenol with strong antioxidant activity, includes eckol and dieckol. Studies on the activity of phlorotannin in seaweed include antithrombotic activity, antioxidant activity, cardiovascular protective effect, antiviral activity, Results such as hyaluronidase inhibitory activity have been reported.

Hyaluronic acid (HA) is a polysaccharide present in the extracellular matrix of connective tissue and is present in the dermis. Polymer HA controls the inflammatory response by inhibiting the phagocytosis of macrophages. Hyaluronidase is an enzyme that breaks down high molecular weight HA and transforms it into low molecular weight HA. Low molecular weight HA is found in high concentrations in patients with inflammatory diseases such as rheumatoid arthritis. Therefore, the development of hyaluronidase inhibitors is an important research goal in the study of anti-inflammatory drugs, and in particular, it is attracting attention in research on atopic dermatitis drugs.

Ecklonia cava extract has an effect of alleviating lipid peroxidation when exposing fine dust PM10 in the air to skin keratinocytes. As a result of examining the expression, the expression of inflammation was significantly reduced. In other words, the ingredients in Ecklonia reduce inflammation caused by fine dust.

grape

Grapes are berries and ripen in July to August to a brownish purple color. It is a widely used fruit because it has a good flavor and is juicy. The place of origin is known to be the Black Sea coast and Kafka region of western Asia, and records about it appear in Korean literature during the Goryeo period. The main production area is Gyeongsangbuk-do, and there are various varieties such as Campbell Early, Muscat Bailey, Black Hamburg, and Delaware. It is mainly used as a raw fruit, and is also used in beverages, brewing, jam, dried fruit, and canned food.

Grape seeds contain 40 times more antioxidants than vitamin E. Grapes are particularly rich in glucose and fructose, and are rich in potassium and iron among minerals, and contain vitamins as well. A special ingredient contained in grapes is polyphenol, which is a benzene ring in the molecule and is attracting attention due to its excellent antioxidant action, and there are many types. Among them, proanthocyanidin, an astringent component present in grape seeds, and resveratrol, present in grape skins. Proanthocyanidins have strong antioxidant power that is 40 times that of vitamin E and 20 times that of vitamin C, so it removes free radicals and inhibits oxidative enzyme activity.

Resveratrol, an active ingredient in grapes, is found in many plants, including berries such as mulberry, peanut, grape, raspberry, and cranberry. It is found more often than other plants because it secretes a powerful antibacterial substance called resveratrol as a defense substance to protect itself. Resveratrol is known to have anticancer and powerful antioxidant properties, and is known to lower serum cholesterol. In addition, it is known to have antiviral, neuroprotective, anti-inflammatory, anti-aging, and life-extending effects. The French paradox is caused by the action of resveratrol and refers to a phenomenon in which French people eat a relatively high-fat diet, but the incidence of heart disease is low. In grape components, resveratrol has a very good antioxidant effect in scavenging DPPH radicals. It also reduces the expression of IL-6, an inflammatory cytokine induced by PM10.

The herbal material used in the extract of the present application includes 1.0 to 2.0 parts by weight of sea swallow's nest, 0.5 to 1.5 parts by weight of pure vegetables, 1.0 to 2.0 parts by weight of kelp, 0.5 to 1.5 parts by weight of Ecklonia cava, and 0.5 to 1.5 parts by weight of grapes. The compounding ratio of the present invention was derived by the present inventors through various kinds of efficacy measurement and feedback, and the content of mucopolysaccharide and antioxidant in the extract is optimized.

The extract of the present application is extracted from the mixture by immersing the above-described herbal medicine in a solvent. The solvent may be water, alcohol, or a mixture thereof. The alcohol may be an alcohol having 1 to 6 carbon atoms. The alcohol may be a polyalcohol having one or more OH groups or two or more OH groups. Specifically, the alcohol may be methanol, ethanol, n-propanol, iso-propanol, n-butanol, tert-butanol, propylene glycol, butylene glycol, dipropylene glycol, or glycerin, but is not limited thereto. According to one embodiment of the present application, the solvent may be a mixed solvent of 1,3-butylene glycol and water, and the concentration of 1,3-butylene glycol may be 20 to 40% (v/v). .

The extract of the present application refers to a solution obtained by eluting the active ingredient from the herbal medicine to the solvent after the herbal medicine is immersed in the solvent. The extraction method may be used without limitation as long as it is an extraction method used in the art, and preferably, an extraction method such as hot water extraction, hot water extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction may be used. According to an embodiment of the present application, low-temperature aging extraction, ultrasonic extraction, or both may be used as the extraction method.

According to one embodiment of the present application, the extract may be obtained by low-temperature aging extraction with respect to the mixture. Specifically, the mixture may be aged at a low temperature at a temperature of 5 to 15 ℃. More specifically, it may be aged at a low temperature at a temperature of 10 to 15 ℃. As it is aged in the above temperature range for a certain period, the elution of high mucopolysaccharide components is possible. According to an experimental example of the present application, the aged ultrasonic extract exhibited higher antioxidant power than the unaged ultrasonic extract, and improved the viability of skin cells due to fine dust and improved anti-inflammatory activity.

According to one embodiment of the present application, the mixture may be stored in an onggi and aged at a low temperature. As used herein, the term “onggi” refers to a non-polluting pottery made by containing 98% or more of natural materials, specifically 99% or more, and more specifically 99.5% or more, and refers to a pot for storing fermented food for a long time. It is made of clay composed of numerous grains of sand, and microscopic air holes are created in the walls of the bowl, making it a container that allows air to pass through the inside and outside of the pottery, and the aging process can occur quickly. In the case of fermentation in Onggi, the production of active ingredients may be increased compared to the case of using glass, PP, PET, and stainless steel containers. According to an exemplary embodiment of the present application, the Onggi may be used in Onggi made from soil in Gangjin, Jeju, Ulsan, Yeoju, or Yesan. According to an embodiment not limited herein, the pottery may be made of strong earthquake soil.

According to one embodiment of the present application, the mixture may be stored and aged in an earthenware for 10 to 30 days. More specifically, the aging may be carried out for 15 to 30 days. As the aging progresses for the above period, the dissolution of polysaccharides and mucilage is maximized. As used herein, the term “mature” is defined as a process in which proteins, fats, carbohydrates, etc., which are constituents of a target material, are decomposed to be beneficial to humans by the action of enzymes, microorganisms, or salts to have unique properties. As a part of the manufacturing process of cosmetics, etc. or as a necessary condition after manufacture, the product with insufficient flavor or texture can be left for some time under certain conditions to have properties suitable for the target cosmetic. In various stages of the processing process, leaving it to stand for a certain period of time until it is in a state suitable for the next processing is also included in aging. During the aging process, changes in constituents occur through the growth of microorganisms, autolysis of target substances, and tissue softening by salts. During the aging process, production and tissue changes of active ingredients occur due to interactions between enzymes, microorganisms, or components, and thus can be preferably used in a cosmetic composition. During the aging process, the density of the tissue is lowered, which affects the solvent affinity of various compounds present in natural products, thereby facilitating the dissolution of soluble substances. Accordingly, it may be changed to be suitable for percutaneous absorption.

According to one embodiment of the present application, the extract of the present invention may be extracted after irradiating the mixture with ultrasonic waves. As used herein, the term “ultrasonic extraction” refers to a method of applying ultrasonic waves of a high frequency of 20 kHz or more in extraction using a solvent. In this case, high energy is generated due to cavitation that occurs as an appropriate level of ultrasonic wave passes through the solvent to rupture the cell wall and internal structure of the cell, thereby allowing the solvent to penetrate into the cell, thereby increasing the extraction efficiency. According to an experimental example of the present application, ultrasonic extract or low-temperature ultrasonic extract showed higher antioxidant power than general hot water extract, and improved viability and anti-inflammatory activity of skin cells caused by fine dust.

Specifically, the extract of the present invention may be obtained by sonicating the mixture with a low-temperature aging treatment. In this case, it is preferable that the ultrasonic wave is irradiated after the low-temperature aging treatment, but it may include a case in which the mixture is subjected to ultrasonic treatment before the low-temperature aging treatment or ultrasonic treatment during low-temperature aging. Ultrasonic extraction can provide a high extraction yield for antioxidants, and thus the sonicated extract of the present invention _{can exhibit high radical scavenging and H 2} O ₂ scavenging activity.

According to one embodiment of the present application, the mixture may be irradiated with ultrasonic waves of 20 to 40 kHz. When an ultrasonic intensity higher than the above range is used, a large amount of unnecessary components may be eluted, and when an ultrasonic intensity lower than the above range is used, the extract content of the antioxidant component may be significantly reduced.

According to one embodiment of the present application, the ultrasonic extraction may be performed for 2 to 4 hours, and when treated for more than or less than the above range, yield or efficacy may be reduced.

According to one embodiment of the present application, the ultrasonic wave may be irradiated at a temperature of 15 to 25 ℃. As the ultrasonic wave is irradiated under a low temperature such as the above range, the active ingredient can be extracted with a high content while minimizing the destruction of the active ingredient by the ultrasonic wave.

According to one embodiment of the present application, the extract of the present invention may be a residual liquid from which the solute is removed after treating the mixture by the above-described extraction method. In addition, the extract of the present invention may be a filtrate obtained by filtering the residue, or a supernatant obtained by leaching the residue or filtrate. In addition, the extract of the present invention may be a concentrate of the residue, filtrate, or a supernatant thereof. The methods of filtration, leaching and concentration may be used without limitation as long as they are methods used in the art. However, according to one embodiment of the present application, filtration may not be performed in order to maximize the content of mucopolysaccharide.

The cosmetic composition of the present application contains the extract of the present invention as an active ingredient to exhibit a skin protection function. Specifically, the extract of the present invention can protect the skin surface and prevent the penetration of fine dust by including the polysaccharide mucus component extracted from sea swallow's nest, pure greens, and kelp in an appropriate amount. In addition, the extract of the present invention can relieve inflammation caused by fine dust in the skin as it contains Ecole and diecol, an active ingredient of Ecklonia cava, and resveratrol, an active ingredient of grapes. Accordingly, the cosmetic composition of the present invention may prevent or improve skin damage caused by fine dust. The five herbal ingredients of the present invention are combined in an optimal content ratio and are suitable for preventing or improving damage caused by fine dust. Furthermore, the extract of the present invention is effective in improving wrinkles and maintaining skin elasticity. In this regard, according to an experimental example of the present application, it was confirmed that the extract of the present invention promotes the proliferation of fibroblasts.

In one embodiment of the present application, the cosmetic composition of the present application may be formulated in various cosmetic formulations. In one embodiment of the present application, the cosmetic formulation composition contains emulsifiers, oils and fats, waxes, surfactants, thickeners, pigments, cosmetic additives, powders, sugars, antioxidants, buffers, and various extracts within the range that does not impair the effects of the present invention. , stabilizers, stimulants, preservatives, moisturizers, fragrances, etc., which are commonly formulated in cosmetic compositions, may be appropriately blended and used.

In one embodiment of the present application, the mixing amount of the active ingredient of the cosmetic may be appropriately determined according to the purpose of use, and the cosmetic composition according to the present invention may contain the extract in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition. have.

As another aspect for solving the above-described problems, the present invention is 1.0 to 2.0 parts by weight of seabird's nest in a solvent, 0.5 to 1.5 parts by weight of pure vegetables, 1.0 to 2.0 parts by weight of kelp, 0.5 to 1.5 parts by weight of Ecklonia cava, and 0.5 parts by weight of grapes To provide a quasi-drug composition for skin protection, comprising, as an active ingredient, an extract extracted from a mixture in which herbal medicines containing 1.5 to 1.5 parts by weight are immersed. The terms used herein are the same as described above.

The term "quasi-drug" as used in the present invention refers to items with a milder action than pharmaceuticals among items used for the purpose of diagnosing, treating, improving, alleviating, treating or preventing diseases of humans or animals, for example, in the Pharmaceutical Affairs Act. According to the definition of quasi-drugs, quasi-drugs are products that are not used for medicinal purposes. Textile and rubber products used for the treatment or prevention of diseases of humans and animals, those that have a slight or no direct action on the human body, and are not instruments or machines, and similar products. , and disinfectants and insecticides to prevent infectious diseases.

The type or formulation of the quasi-drug composition of the present invention is not particularly limited, but preferably disinfectant, shower foam, gargrin, wet tissue, detergent soap, hand wash, humidifier filler, mask, ointment or filter filler.

When the composition of the present invention is included in a quasi-drug for the purpose of protecting the skin against reactive oxygen species and fine dust, the composition can be used as it is or used together with other quasi-drug ingredients, and can be used appropriately according to a conventional method. . The mixing amount of the active ingredient may be suitably determined according to the purpose of use, and the quasi-drug composition according to the present invention may contain the extract in an amount of 0.01 to 20% by weight based on the total weight of the quasi-drug composition.

As another aspect for solving the above-described problems, the present invention is 1.0 to 2.0 parts by weight of seabird's nest in a solvent, 0.5 to 1.5 parts by weight of pure vegetables, 1.0 to 2.0 parts by weight of kelp, 0.5 to 1.5 parts by weight of Ecklonia cava, and 0.5 parts by weight of grapes To provide an external composition for skin protection comprising an extract extracted from a mixture in which herbal medicines containing 1.5 to 1.5 parts by weight are immersed as an active ingredient. The terms used herein are the same as described above.

As used herein, the term “external preparation” refers to application to the skin among pharmaceuticals used for the purpose of diagnosing, treating, improving, alleviating, treating or preventing diseases of humans or animals. Specifically, the external preparation of the present invention may have a preventive or therapeutic use for dermatitis, and may be particularly suitable for dermatitis caused by fine dust stimulation.

The composition for external application according to the present invention includes, for example, ointment, lotion, solubilization, suspension, emulsion, cream, gel, spray, patch, warning agent, patch, or water paste, but is not limited thereto. , can be formulated in any base well known in the art. The composition for external application for skin according to the present invention may contain the extract in an amount of 0.01 to 20% by weight based on the total weight of the composition for external application.

As another aspect for solving the above-described problems, the present invention is 1.0 to 2.0 parts by weight of seabird's nest in a solvent, 0.5 to 1.5 parts by weight of pure vegetables, 1.0 to 2.0 parts by weight of kelp, 0.5 to 1.5 parts by weight of Ecklonia cava, and 0.5 parts by weight of grapes A first step of immersing the herbal material containing 1.5 to 1.5 parts by weight; It can provide a method for producing a herbal extract for skin protection, comprising a second step of extracting the extract from the immersed mixture. The terms used herein are the same as described above.

The second step may include the aforementioned low-temperature aging and/or ultrasonic extraction steps.

The extract according to the present invention can effectively prevent the penetration of fine dust into the skin because it contains abundantly polysaccharide mucus component.

In addition, the extract according to the present invention contains antioxidant components such as phlorotannin and resveratrol such as eckol and dieckol, and can suppress free radicals and inflammation-inducing factors induced by fine dust.

Furthermore, the extract according to the present invention not only improves skin irritation caused by fine dust, but also maintains skin elasticity and has an excellent effect in improving wrinkles.

Accordingly, the extract of the present invention can be used as a cosmetic, quasi-drug, or external preparation for skin protection from fine dust.

The effects of the present invention are not limited to the above effects, but it should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a schematic diagram showing a process for preparing an extract according to an embodiment of the present application.

Hereinafter, the present invention will be described with reference to the accompanying drawings. However, the present invention may be embodied in several different forms, and thus is not limited to the embodiments described herein.

The terms used herein are used only to describe specific embodiments, and are not intended to limit the present invention. The singular expression includes the plural expression unless the context clearly dictates otherwise. In this specification, terms such as "comprises" or "have" are intended to designate that the features, numbers, steps, operations, components, parts, or combinations thereof described in the specification exist, but one or more other features It should be understood that this does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Preparation Example 1. Preparation of aged extracts containing sea swallow's nest, pure vegetables, kelp, Ecklonia cava, and grapes

The herbal medicine of the present invention, Swallow's nest, was purchased from China, and Korean vegetables, kelp, kelp, and grapes were purchased. The herbal ingredients were dried in the shade before mixing. Bird's nest, leaves and stems of pure vegetables, stems of kelp, leaves and stems of Ecklonia cava, and grapes were mixed in a weight ratio of 1.5:1:1.5:1:1, and 1,3-butylene glycol 30% aqueous solvent It was immersed in a weight ratio of 1 (solute):30 (solvent).

The soaked mixture was extracted in the manner described in Table 1 below.

In Table 1, the general hot water extract shows the extract obtained by heating and extracting the immersed mixture under conditions of 80 to 90° C. for 2 hours.

In addition, the ultrasonic extract represents an extract obtained by applying ultrasonic waves of 20 kHz at 25° C. to the immersed mixture at room temperature for 1.5 hours.

In addition, the low-temperature ultrasonic extract represents an extract obtained by applying ultrasonic waves of 20 kHz to the immersed mixture at a temperature of 15 to 20° C. for 1.5 hours.

Aged low-temperature ultrasonic extract is obtained by putting the immersed mixture in a pottery in Gangjin and aging it for 15 days while maintaining 10 to 15 ° C in a Gwangcheon crypt, and then applying ultrasonic waves at 20 kHz under a temperature of 15 to 20 ° C for 1.5 hours. The extract obtained by extraction is shown.

extract 1 extract 2 extract 3 extract 4 extraction method General hot water extraction Ultrasonic Extraction Low-temperature ultrasound extraction Aged Cryogenic Ultrasonic Extraction Swallow's nest 1.5 1.5 1.5 1.5 pure vegetables One One One One Kelp 1.5 1.5 1.5 1.5 Ecklonia One One One One grape One One One One

After the extraction, the solute part (natural material) was removed, and the remaining residue was used as a supernatant (supernatant was approximately 85-90 vol%) by inducing leaching. In the supernatant, mucopolysaccharides were formed, which contained a large amount of active ingredients, so that separate filtration was not performed.

Experimental Example 1 - Measurement of antioxidant power (electron donating ability test)

In order to measure the antioxidant power of the extract, the free radical scavenging rate using DPPH was measured. DPPH was used diluted in methanol to a concentration of 0.2 mM. Extracts 1 to 4 were diluted in DW for each concentration (final concentration 0.1%, 0.5%, 1.0% (v/v)) and used. 100 μl of the sample for each concentration of Example 1 and 50 μl of the DPPH solution were sequentially added to the 96-well plate, followed by reaction at 37° C. for 30 minutes. After the reaction, absorbance was measured at 517 nm using a microplate reader. As a blank sample solution, DW was used instead of a sample, and ascorbic acid 100 μg/ml was used as a positive control. The results are shown in Table 2 below.

Treatment concentration (v/v) 0.1% 0.5% One% extract 1
(hot water extraction) 12.7 37.4 65.7 Extract 2 (ultrasonic extraction) 18.1 42.1 72.5 Extract 3 (Cryogenic Ultrasound) 17.3 41.2 75.4 Extract 4 (aged cryogenic ultrasound) 32.7 52.8 85.7 Ascorbic acid 100ug/ml 82.7%

Extracts 1 to 4 were confirmed to exhibit antioxidant activity in a concentration-dependent manner. In particular, it was confirmed that extract 4 at a concentration of 1% exhibited higher antioxidant power than that of ascorbic acid.

Experimental Example 2 - Measurement of antioxidant power (ABTS radical scavenging ability test)

For the measurement of antioxidant power, the degree of conversion of an antioxidant candidate to colorless ABTS by scavenging the blue-green radical ABTS (2,2'-azinodi [3-ethlbenzthiazolne-6-sulfonic acid]) is measured at 732 nm. A scavenging ability test was performed. Extracts 1 to 4 were diluted in DW at each concentration (final concentration 0.1%, 0.5%, 1.0% (v/v)) to measure radical ABTS scavenging ability. As a positive control, 100 μg/ml of ascorbic acid was used. The results are shown in Table 3 below.

Treatment concentration (v/v) 0.1% 0.5% One% extract 1
(hot water extraction) 12.4 39.8 68.6 Extract 2 (ultrasonic extraction) 20.8 37.8 71.8 Extract 3 (Cryogenic Ultrasound) 15.6 35.5 69.4 Extract 4 (aged cryogenic ultrasound) 28.1 48.7 88.1 Ascorbic acid 100ug/ml 84.4%

Extracts 1 to 4 were confirmed to exhibit antioxidant activity in a concentration-dependent manner. In particular, it was confirmed that extract 4 at a concentration of 1% exhibited higher antioxidant power than that of ascorbic acid.

Experimental Example 3 - Skin cell protective effect

Extracts 1 to 4 were added during culturing of normal human fibroblasts, and the skin cell protective effect was measured by the MTT assay method.

Fibroblast HS86 was inoculated at a density ^{of 1×10 4} cells in DMEM (Dulbecco's Modified Eagle's Medium) supplemented with 10% bovine serum, and cultured _{at 5% CO 2} , 37° C. for one day. The extract samples were diluted in a serum-free medium for each concentration shown in Table 4, treated or untreated (fine dust alone), and incubated for 30 minutes, and NIST ^® SRM ^® 1648a, a fine dust substance as an irritant, (about 25 types). of heavy metal) was added at 50 μg/ml and cultured for 1 day. Then, after treatment with MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), a dye for cell staining, the cells were further cultured for 4 hours. Chromosomes were dissolved with dimethyl sulfoxide, and absorbance was measured at 540 nm for relative comparison. As a control group, a culture medium (untreated group) that was not treated with fine dust stimulation and samples was used.

Cell viability (%) = 100 - (absorbance of each extract sample)/(absorbance of control group)*100 to calculate the cell viability, and the group grown without treating the extract sample and fine dust material The relative cytoprotective effect when the cell proliferation rate (%) of (untreated group) was 100 is shown in Table 4 below.

Treatment concentration (v/v) 0.10% 0.50% 1.00% 3.00% extract 1
(hot water extraction) 68.4 75.6 81.1 90.4 Extract 2 (ultrasonic extraction) 81.9 82.3 94.2 97.4 Extract 3 (low temperature first wave) 80.1 87.5 95.4 98.7 Extract 4 (aged cryogenic ultrasound) 84.6 98.7 100.1 102.8 untreated group 100 Fine dust treatment group 64.8

As described above, it was confirmed that extracts 1 to 4 exhibited an excellent cytoprotective effect compared to the group in which fine dust was added and no extract was added. In particular, in the case of extract 4, it was confirmed that it proliferated in a larger amount than the cell group proliferated without adding fine dust.

Experimental Example 4 - Anti-inflammatory activity test

Extracts 1 to 4 obtained in Preparation Example 1 were added during culturing of normal human fibroblasts to evaluate the ability to inhibit the expression of inflammatory factors TNF-α and IL-6.

Specifically, fibroblasts (Fibroblast HS86) were inoculated in a 48-well plate and cultured for 24 hours. ^{After replacing the medium and treating NIST ®} SRM ^® 1648a (containing about 25 kinds of heavy metals), which is a fine dust substance as an irritant, at 50 μg/ml, extracts 1 to 4 were treated with the concentrations shown in Tables 5 and 6 below or After non-treatment (fine dust alone treatment group), culture was performed for 48 hours. The % of the concentration of TNF-α in the extract-treated culture medium was analyzed compared to the comparative culture medium (untreated group) that was not treated with fine dust substances. The concentrations of TNF-α and IL-6 were measured by ELISA. The results for the inhibition rate (%) of TNF-α and IL-6 are shown in Tables 5 and 6, respectively.

Treatment concentration (v/v) 0.10% 0.50% One% extract 1
(hot water extraction) 92.5 62.2 46.1 Extract 2 (ultrasonic extraction) 91.2 59.4 34.8 Extract 3 (low temperature first wave) 88.1 50.7 34.1 Extract 4 (aged cryogenic ultrasound) 81.3 45.1 28.7 untreated group 12.3 Fine dust treatment group 97.5

treatment concentration 0.10% 0.50% One% hot water extraction 94.8 71.4 49.7 Ultrasonic Extraction 87.4 56.4 35.9 low temperature first wave 90.5 55.4 36.8 Aged cryogenic ultrasound 79.4 44.3 25.1 untreated group 9.4 Fine dust SRM1648a 50ug/ml 98.4

From the above results, it was found that extracts 1 to 4 had an inhibitory activity of inflammation caused by fine dust in a concentration-dependent manner. In particular, in the case of extract 4, there was a very good inhibitory activity against inflammation stimulated by fine dust.

Experimental Example 5 - Wrinkle improvement measurement test

In order to determine whether the wrinkle improvement of the extracts 1 to 4, an elastase inhibition assay was performed. Specifically, the following experiments were performed.

Elastase is an enzyme that breaks down elastin, which exists in the dermis and maintains the elasticity of the skin. Elastase 　 inhibitory activity was measured using arbutin, known as an elastase inhibitor, as a positive control.

0.2M Tris-HCl (pH 8.0) was mixed with 80 μl of 3.2 mM Succ-Ala-Ala-Ala-p-nitroanilide, and 100 μl of a sample having a concentration in Table 7 below. Here, 1U of human leukocytes elastase was dissolved in 1 ml of 0.2 M Tris-HCl (pH 8.0), and then 50 μl (50 mU) was added thereto. Enzyme activation reaction was performed at 25°C for 15 minutes. After the reaction, the absorbance values of the control and each sample were measured at 415 nm using an ELISA reader.

0.10% 0.50% 1.00% extract 1
(hot water extraction) 11.8 38.9 52.6 Extract 2 (ultrasonic extraction) 12.4 40.7 53.7 Extract 3 (low temperature first wave) 12.8 41.1 53.4 Extract 4 (aged cryogenic ultrasound) 15.4 43.8 64.5 Arbutin 1.0% 83.5% active

As a result, as shown in Table 7, extracts 1 to 4 showed excellent anti-wrinkle effect. In particular, in the case of extract 4, it was found that the wrinkle improvement effect was significantly increased compared to the other extracts.

The above description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention.

Claims (10)

Extracts extracted from a mixture in which herbal medicines containing 1.0 to 2.0 parts by weight of seabird's nest, 0.5 to 1.5 parts by weight of pure green vegetables, 1.0 to 2.0 parts by weight of kelp, 0.5 to 1.5 parts by weight of Ecklonia, and 0.5 to 1.5 parts by weight of grapes are immersed in a solvent A cosmetic composition for skin protection comprising as an active ingredient.
The cosmetic composition according to claim 1, wherein the mixture is aged at a low temperature at a temperature of 5 to 15°C.
The cosmetic composition according to claim 2, wherein the mixture is stored in an earthenware for 10 to 30 days and aged at a low temperature.
The cosmetic composition according to claim 1, wherein the mixture is irradiated with ultrasonic waves of 20 to 40 KHz.
The cosmetic composition according to claim 4, wherein the ultrasonic waves are irradiated at a temperature of 15 to 25°C.
The cosmetic composition according to claim 1, wherein the solvent is water, alcohol, or a mixed solvent thereof.
The cosmetic composition according to claim 1, wherein the cosmetic composition prevents or improves skin damage caused by fine dust.
Extracts extracted from a mixture immersed in a solvent in which herbal medicines containing 1.0 to 2.0 parts by weight of sea swallow's nest, 0.5 to 1.5 parts by weight of pure vegetables, 1.0 to 2.0 parts by weight of kelp, 0.5 to 1.5 parts by weight of Ecklonia chai, and 0.5 to 1.5 parts by weight of grapes are immersed in a solvent. A quasi-drug composition for skin protection, comprising as an active ingredient.
Extracts extracted from a mixture immersed in a solvent in which herbal medicines containing 1.0 to 2.0 parts by weight of sea swallow's nest, 0.5 to 1.5 parts by weight of pure vegetables, 1.0 to 2.0 parts by weight of kelp, 0.5 to 1.5 parts by weight of Ecklonia chai, and 0.5 to 1.5 parts by weight of grapes are immersed in a solvent. A composition for external application for skin protection, comprising as an active ingredient.
A first step of immersing a herbal medicine containing 1.0 to 2.0 parts by weight of bird's nest in a solvent, 0.5 to 1.5 parts by weight of pure vegetables, 1.0 to 2.0 parts by weight of kelp, 0.5 to 1.5 parts by weight of Ecklonia cava, and 0.5 to 1.5 parts by weight of grapes;
A method for producing a herbal extract for skin protection, comprising a second step of extracting the extract from the immersed mixture.

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