KR20180020571A - Cosmetic compositon for removing heavy metal or fine dust containing extracts of ficus religosa - Google Patents

Abstract

According to an embodiment of the present invention, provided is a cosmetic composition for removing heavy metal or fine dust which contains extracts of Ficus religiosa trees as effective components. Therefore, the cosmetic composition has excellent absorbing power with respect to heavy metal or fine dust.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for removing heavy metals or fine dusts containing an extract of Liliaceae bark extract as an active ingredient.

More particularly, the present invention relates to a cosmetic composition having excellent effects of adsorbing and removing heavy metals or fine dust remaining in skin pores.

Rapid industrialization, automobile exhaust gas, heavy metal dusts caused by air masses moved from China, and foreign pollutants such as fine dust pollute people's skin and are considered to be major cause of skin aging and trouble.

Heavy metals are heavy metals such as lead, mercury, cadmium, zinc, and nickel, which have a specific gravity of 4 or more. These heavy metals accumulate in the body, accumulate in proteins in the body, It is perceived as dangerous. In addition, it has been reported that a chemical reaction occurs in the air in the skin to generate harmful substances such as nitrogen oxides (NO) to cause skin inflammation, atopy, and skin troubles.

In addition, fine dust refers to a small dust less than 10 μm in diameter which is invisible and is known to contain toxic substances such as sulfate and nitrate. These fine dusts are produced by the photochemical reaction in the air such as toxic substances or heavy metals from automobile soot and factory smokes. When exposed to a high concentration of fine dust environment, cough, eye sting, skin trouble, Lt; / RTI >

At present, there is a method of removing heavy metals from the body by injecting a complex-forming substance such as EDTA (ethylene diamine tetraacetic acid) and BAL (British anti-lewisite) to bind heavy metals accumulated in the body, Also, there is a method of preventing the absorption of heavy metals by the body by taking vitamin B1, vitamin C and vitamin E and facilitating the discharge. However, there is no effective way to remove the heavy metals accumulated in the pores of the skin. Fine dust can be removed by cleansing to a certain extent, but since the adsorption force is stronger as it is finer, it penetrates deeply into the pores and is completely removed by general cleansing. It is impossible to do.

Meanwhile, in the cosmetics industry, many products using natural products have been developed to reduce skin irritation caused by various chemical substances. In addition to low adverse effects on skin, natural materials have recently become increasingly appreciated as a cosmetic raw material as consumers' response to cosmetics using natural materials has increased. Accordingly, there is a demand for development of a cosmetic composition having excellent adsorbability of heavy metals and fine dusts while enhancing skin stability, including extracts based on natural materials.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems occurring in the prior art, and an object of the present invention is to provide a biodegradable, To provide a cosmetic composition.

An aspect of the present invention provides a cosmetic composition for the removal of heavy metals or fine dusts comprising an extract of Indian Borax Seeds as an active ingredient.

In one embodiment, the extract may contain a fermentation extract prepared by inoculating a fermentation strain.

In one embodiment, the composition may comprise a resin extract of Indian bamboo.

In one embodiment, the resin extract may include a fermentation extract prepared by inoculating a fermentation strain.

In one embodiment, the fermentation strain is selected from the group consisting of Lactobacillus sp. , Monascus sp. , Bifidobacterium sp. , Prevotella sp. , Peugeot bacteria, Fusobacterium sp. , And Eubacterium sp. , Respectively .

In one embodiment, the extract is selected from the group consisting of water, anhydrous or lower alcohols having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, Glycol, < / RTI >

In one embodiment, the alcohol may be ethanol at a concentration of 65-75% (v / v).

In one embodiment, the cosmetic composition is selected from the group consisting of antioxidants, anti-inflammation, skin elasticity improvement, skin hydration control, collagen increase, skin barrier recovery, skin texture improvement, skin wrinkle reduction, DNA damage inhibition and skin erythema reduction One or more skin-improving effects.

In one embodiment, the cosmetic composition is comprised of a softening agent, a nutritional lotion, a water cream, a nutritional cream, a massage cream, an essence, an ampoule, a gel, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, Lt; / RTI > may be formulated into one or more selected from the group.

According to the present invention, the cosmetic composition of the present invention is excellent in skin-improving effect because it contains an extract of banyan tree, that is, an extract derived from a natural plant, as an active ingredient, and can improve the adsorption and removal effects of heavy metals or fine dusts .

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and similar parts are denoted by like reference characters throughout the specification.

Throughout the specification, when a part is referred to as being "connected" to another part, it includes not only "directly connected" but also "indirectly connected" . Also, when an element is referred to as "comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

Hereinafter, embodiments of the present invention will be described in detail.

An aspect of the present invention provides a cosmetic composition for the removal of heavy metals or fine dusts comprising an extract of Indian Borax Seeds as an active ingredient.

The above-mentioned " Ficus religiosa " is a plant belonging to the bamboo tree species and is generally called bamboo tree. It is believed to be a sacred tree because the Buddha realized under the bamboo tree. In Korea, it is mainly cultivated as a pipe or in fruit trees and distributed in the wild. Trees are not toxic and can be used for medicinal or edible purposes such as fruit, flowers, leaves, stems and roots. The fruit of barley is a pointy nucleus, long elliptical, 1.5㎝ in length, and ripened in red from June to July. It has a bitter taste and sweet taste and can be edible. In one room, it is said to berry the fruit of the bamboo fruit. Its efficacy protects the five stomach, eliminates blistering and burning, stops diarrhea and bleeding, and causes dyspepsia, osteomyelitis, edema, It is known that there is a medicinal effect.

The leaves and stems of the bamboo trees have scaly silver-white hairs and grow at an altitude of less than 1,200 meters. Because of this, there is nitrogen fixed bacteria in the root of the bamboo tree, and it grows well in the barren soil, and grows well without giving pesticide, chemical fertilizer, and special management. It has adsorbability to heavy metals such as lead, cadmium, cobalt, nickel, and chrome. In addition, it has excellent anti-inflammatory, antibacterial and anti-ulcer effects.

Though it is expected to be a fertilizer that can be compared to organic and organic fruits, it is now cultivated as ornamental water, but fruit cultivation is hardly achieved. In addition, borealum is cultivated only in Korea and Japan, and it is the only fruit that can cope with the WTO and FTA. As a result of research on nutrients and functional materials and development of processed food, it is possible to increase the value added of agricultural products and farm income It is expected to be popular as a substitute crop.

The lime extract may be prepared by mixing lime juice crushed by an extraction method known in the art into an extraction solvent and then extracting it. Wherein the extraction solvent is selected from the group consisting of water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3- The solvent may be one or more selected daily, preferably ethanol at a concentration of 65 to 75% (v / v), and two or more different solvents may be mixed or used sequentially, but the present invention is not limited thereto.

That is, the extraction ratio may be different depending on the polarity of the solvent, and the ethanol is excellent in the selectivity in the extraction of the physiologically active substance of the natural raw material. Therefore, Can be implemented.

Particularly, the water and ethanol have different polarities, and the effective components extracted according to the respective polarities may be different from each other, so that the concentration of the ethanol can be appropriately controlled so that an optimum skin improving effect can be realized. However, when the concentration of ethanol is more than 75%, an adequate yield may not be realized. If the concentration is less than 65%, the effective ingredient showing skin improving effect may not be extracted sufficiently. Therefore, It can be suitably adjusted, and preferably it can be ethanol at a concentration of 70%.

In one embodiment, the composition may comprise a resin extract of Indian bamboo.

As used herein, the term " resin extract " means an extract obtained by extracting the resin of the Indian bloom tree with an organic solvent such as hexane, petroleum ether and the like. By extracting the Indian bamboo wood resin with an organic solvent, various lipid soluble components contained in the Indian bamboo wood resin can be effectively extracted, and the skin improving effect can be remarkably improved. The organic extraction solvent may be one or more solvents selected from the group consisting of acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, chloroform, hexane and 1,3-butylene glycol.

In one embodiment, the extract may be a fermented extract prepared by inoculating a fermentation strain into the extract of the Indian Borax seed tree or the extract of the Indian bamboo tree plant.

The term " fermentation " as used herein means a process in which a microorganism decomposes an organic substance using an enzyme contained therein. It is known that raw materials after fermentation through microorganisms are at least two times to several tens of times more effective than before fermentation. Fermented metabolites contain various amino acids, organic acids and antioxidants that are good for the skin, promoting skin metabolism and making skin texture resilient and smooth. In addition, as the fermentation process proceeds, the particles become smaller and the toxicity such as heavy metals is decomposed, so that the absorption rate is good, and skin troubles and allergy side effects can be alleviated.

The "fermentation extract" used in the present invention is prepared by drying the extract of Indian Borax seedlings or Indian bamboo tree resin naturally or by using an apparatus, specifically a rotary vacuum concentrator and a freeze drier, and then drying the extract or the resin extract in a solvent, Preferably diluted to a predetermined concentration in purified water, inoculated with a fermentation strain and fermented.

In one embodiment, the fermentation strain is selected from the group consisting of Lactobacillus sp. , Monascus sp. , Bifidobacterium sp. , Prevotella sp. , Peugeot bacteria, Fusobacterium sp. , And Eubacterium sp. , Respectively .

In one embodiment, the cosmetic composition is selected from the group consisting of antioxidants, anti-inflammation, skin elasticity improvement, skin hydration control, collagen increase, skin barrier recovery, skin texture improvement, skin wrinkle reduction, DNA damage inhibition and skin erythema reduction One or more skin-improving effects.

In one embodiment, the cosmetic composition is comprised of a softening agent, a nutritional lotion, a water cream, a nutritional cream, a massage cream, an essence, an ampoule, a gel, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, Lt; / RTI > may be formulated into one or more selected from the group.

The cosmetic composition may be appropriately blended with other ingredients within the range of not hindering the object of the present invention depending on the type of the active ingredient, the purpose of use, and the like in each formulation.

Also, depending on the quality or function of the final product, there may be used various additives such as a fatty substance, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, A surfactant, a stabilizer, a stabilizer, an antioxidant, an antioxidant, an antioxidant, a preservative, a barrier agent, a wetting agent, an essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, And may further comprise adjuvants commonly used in cosmetics or dermatology, such as any of the other ingredients.

However, it is preferable that the adjuvant and the mixing ratio thereof are appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

Hereinafter, the present invention will be described in further detail with reference to Examples, but it should be apparent that the present invention is not limited by the following Examples.

Example 1: Extracts from Indian Borax

100 g of crushed indian bamboo trees were mixed with 1 L of 70% ethanol at a ratio of 10% by weight. The mixed raw materials were stirred at room temperature for 48 hours, and then filtered through 250 mesh and 0.5 mu m filter paper to obtain a liquid extract. The obtained liquid extract was subjected to a rotary vacuum concentrator and a freeze-dryer to obtain an extract of Lycoris spp., Which was used as a sample of Example 1.

Example 2: Fermented extract of Indian bamboo tree

The extract of Bombyx mori of Example 1 was diluted to 5% by weight with purified water and fermented by inoculation with a fermentation strain, Lactobacillus plantarum. The fermented extract was filtered through 500 mesh and 0.2 mu m filter paper to obtain an extract of a fermented indian bamboo tree, which was used as a sample of Example 2.

Example 3: Indian bamboo tree resin extract

100 g of the resin of the shredded Indian bamboo tree was filled into Thimble at a volume of 20% of hexane, heated to 120 캜, refluxed five times, and filtered through 250 mesh and 0.5 탆 filter paper to obtain a liquid extract. The resulting liquid extract was subjected to a rotary vacuum concentrator and a freeze-dryer to obtain an extract of Indian Borax wood, which was used as a sample of Example 3.

Example 4: Preparation of fermented extract of Indian bamboo tree resin

The extract of Bombyx mori of Example 3 was diluted to 5% by weight with purified water and then fermented by inoculation with a fermentation strain, Lactobacillus plantarum. The fermented extract was filtered through 500 mesh and 0.2 mu m filter paper to obtain a fermented extract of an Indian boreal tree resin, which was used as a sample of Example 4.

Experimental Example 1: Evaluation of inhibitory effect on inflammatory cytokine synthesis

In order to verify the skin-improving effect of the extracts of Examples 1 to 4 prepared according to one embodiment of the present invention, an inhibition of inflammatory cytokine synthesis was carried out.

First, the extracts of Examples 1 to 4 were treated with different concentrations of immune cells IL-1β, IL-6 and TNF-α stimulated by PAHs (polyaromatic hydrocarbons), cultured for 24 hours, -PCR.

SYBR Green I (Invitrogen) was used to quantitatively measure changes in IL-1β, IL-6 and TNF-α gene expression by the above extracts through fluorescence values emitted by PCR products Respectively. The reaction solution was prepared by mixing 0.2 μM primer, 50 mM KCl, 20 mM Tris / HCl pH 8.4, 0.8 mM DNTP, 0.5 U Extaq DNA polymerase, 3 Mm MgCl ₂ and 1 X SYBR Green in PCR tubes. The denaturation, annealing and polymerization were carried out at 94 ° C. for 30 seconds, at 60 ° C. for 30 seconds, at 72 ° C. for 30 seconds, The fluorescence intensity was measured after completion of each cycle.

The Ct value of each gene was normalized to the Ct value of? -Actin, and the amount of change in the Ct value was compared and analyzed. The Ct value is the number of cycles when the amount of fluorescence generated by the PCR product (the number of amplified PCR products) reaches a certain reference value above the basal value, and the amount of gene expression was confirmed through the Ct value. The results are shown in Table 1 below.

[mRNA expression level (multiples)] division Extract concentration (mg / L) Example 1 Example 2 Example 3 Example 4 IL-1? 0 6.2 6.2 6.2 6.2 25 4.9 4.7 4.5 4.0 50 4.2 4.0 3.7 3.4 75 3.7 3.4 3.0 2.2 100 2.5 2.3 2.1 1.7 IL-6 0 13.8 13.8 13.8 13.8 25 7.6 7.4 7.2 6.5 50 6.4 6.1 5.9 5.2 75 5.4 5.2 4.7 4.2 100 5.0 4.7 4.1 3.7 TNF-a 0 4.9 4.9 4.9 4.9 25 4.1 3.8 3.6 3.0 50 3.9 3.6 3.1 2.5 75 3.8 3.2 2.4 2.1 100 3.5 3.0 2.1 1.8

Referring to Table 1, it was confirmed that expression of IL-1β, IL-6 and TNF-α genes increased by PAHs was remarkably reduced after treatment with the extract of Borys waters (Examples 1 to 4). The inhibition of all gene expression was inhibited by increasing the concentration of the extracts. Especially, the inhibition of gene expression was inhibited most effectively when treated with the fermented extract of bamboo trees.

Experimental Example 2: Evaluation of NF-kB transcriptional inhibitory effect

In order to verify the skin-improving effect of the extracts of Examples 1 to 4 prepared according to one embodiment of the present invention, the transcription inhibition experiment of NF-Kb, which is a transcription factor of inflammatory cytokine, was carried out.

First, the promoter region of a gene known to be transcribed by NF-kB was cloned into a vector containing a luciferase gene and inserted into the immune cells. PAHs inducing NF-Kb transcription were treated to activate NF-kB transcriptional activity, and then treated with the extracts of Boriardia solu, Bombyx mori, Borax seedlings and Borax seedlings in Examples 1 to 4 And cultured for 24 hours. The cultured immune cells were directly added with 0.1 mL dissolution buffer (0.1 M HEPES, pH 7.6, 1% Triton-X, 1 mM DTT, and 2 mM EDTA) at the time of collection to collect the cell lysate, The cells were centrifuged at rpm to obtain cell proteins.

Protein concentration was measured using a Bradford assay (Bio-Rad Laboratories), and then 20 μg of protein was added to a luciferase assay mixture (25 mM glycylglycine, 15 mM MgSO ₄ , 1 mg / mL BSA , 5 mM ATP and 1 Mm D-luciferin (Analytical Luminescence Laboratory), and the average value was obtained by repeating the luminescence three times for 20 seconds using Monolight 2010 (Analytical Luminescence Laboratory) The results are shown in Table 2 below.

[Luciferase activity (multiples)] Concentration of lime extract (mg / L) Example 1 Example 2 Example 3 Example 4 NF-kB 0 32.4 32.4 32.4 32.4 25 24.7 23.7 22.4 20.3 50 22.5 20.4 18.7 16.3 75 8.2 7.6 7.1 6.5 100 5.3 4.9 4.6 3.7

Referring to Table 2, the activity of luciferase increased by PAHs was remarkably decreased after treatment with water extract (Examples 1 to 4), and the effect of inhibiting NF-kB transcription was excellent. The activity of luciferase was decreased with increasing concentration of extracts. Especially, the activity of luciferase was most effectively decreased when treated with the fermented extract of bamboo trees.

Experimental Example 3: Polycyclic Aromatic Hydrocarbon Removal Test

The adsorption effect of polycyclic aromatic hydrocarbons (PAH), which is a harmful atmospheric substance contained in a large amount of fine dust, was tested.

Porcine sheath (1 cm x 1 cm) with hair removed was prepared and washed with PBS for 10 minutes. After washing, 600ppm of Naphtalene, Acenaphthylene, Acenaphthene, Fluorene, Fluoranthene, Pyrene, Chrysene, Benzopyrene (PH 11) for 2 hours and then dried at room temperature. The control group was applied with PBS and reacted for 3 hours.

After washing with PBS, the specimen was cut to a predetermined weight and then crushed with a homogenizer. The pulverized tissue was dried with anhydrous sodium sulfate (Na ₂ SO ₄ ), and the polycyclic aromatic hydrocarbons were extracted from the tissue by Sohxlet extraction and liquid-liquid extraction (Husain et al. 1997; Takatsuki et al., 1985) and quantitated by HPLC. The quantitative results are shown in Table 3 below, and the results are expressed as a ratio of the control group to the experimental group.

[% of control] division Example 1 Example 2 Example 3 Example 4 One
mg / L 10
mg / L 50
mg / L One
mg / L 10 mg / L 50 mg / L One
mg / L 10
mg / L 50
mg / L One
mg / L 10
mg / L 50
mg / L naphthalene 28.6 14.4 2.1 29.7 15.2 2.5 31.8 16.9 2.7 32.9 17.4 3.4 Acenaphthylene 33.1 15.8 1.5 34.8 16.2 1.9 37.6 19.6 2.3 39.1 20.7 3.5 Fluorene 34.8 16.9 3.5 39.7 19.2 3.9 42.6 20.7 4.1 43.6 22.7 4.6 Fluoranthene 33.6 15.7 2.6 38.3 16.2 2.5 40.1 18.4 3.2 41.7 23.8 4.1 Pyrene 31.2 12.8 0.6 36.8 14.6 1.5 37.2 15.9 1.9 38.4 16.2 2.6 Crissen 27.2 14.2 1.2 30.4 15.4 1.3 31.9 16.2 1.7 33.4 18.1 2.0 Benzopyran 18.2 6.2 3.1 20.5 7.3 3.3 21.9 8.1 3.9 22.6 8.4 4.3

As a result of the analysis, the polycyclic aromatic hydrocarbons were removed in a concentration-dependent manner according to the treatment of the water extract (Examples 1 to 4), and the results showed that the extract of the water of lily of the lake was contained in fine dust and air, Suggesting that it is possible to effectively remove the substance.

Experimental Example 4: Skin Stability Test

In order to examine the skin stability of the extracts of Examples 1 to 4 prepared according to one embodiment of the present invention, animal skin cells were subjected to MTT assay (measurement of living cell number).

First, skin cells of animals were placed in a medium, and the concentrations of the extracts of Borax seedlings, Borax seedlings, Borax seedlings, Borax seedlings and Borax seedlings were changed to 3 minutes and 1 The cell viability was measured after different time. The results are shown in Table 4 below.

[Cell survival rate (%)] time Concentration of lime extract (mg / L) Example 1 Example 2 Example 3 Example 4 3 minutes 0 102 103 101 103 0.5 101 100 101 101 One 101 102 100 100 2.5 102 101 103 100 5 100 100 100 102 1 hours 0 101 104 101 100 0.5 102 102 102 101 One 101 104 101 101 2.5 100 100 100 104 5 101 101 100 100

The results are shown in Table 4. As shown in Table 4, it was confirmed that the skin cells of Example 1 to 4 did not show apoptosis caused by the extracts of Boriardia tuberosum, Fermented Borealis tree, Extract of Borealis resin, and Fermented Borealis resin.

Experimental Example 5: Examination of eye stability

In order to verify the ocular stability of the extracts of Examples 1 to 4 prepared according to one embodiment of the present invention, an MTT assay (measurement of living cell number) was carried out using SIRC cells as retinal cells of animals.

First, SIRC cells were put into a medium, and the cell viability was measured after varying the concentration of the extracts of Boriardiana, Baiorobee extract, Borax seedlings resin and Borax seedlings resin fermentation extracts of Examples 1 to 4. The results are shown in Table 5 below.

[Cell survival rate (%)] Concentration of lime extract (mg / L) Example 1 Example 2 Example 3 Example 4 0 101 103 101 102 0.5 97 98 99 99 50 80 85 84 91

As shown in Table 5, it was confirmed that the cell death of the bamboo extracts of the examples 1 to 4, the fermentation extract of the bamboo trees, the bamboo tree resin extract and the fermentation extract of the bamboo tree resin was more than 70% .

Experimental Example 6: Evaluation of skin erythema improvement

Experiments were conducted to verify the skin erythema-improving effect of the mask packs prepared from the water extracts of Examples 1 to 4 according to one embodiment of the present invention, and the mask packs were prepared as shown in Table 6 below. In Comparative Examples 1 and 2, groups in which MCT oil and camellia oil were respectively reacted as a control group were introduced.

[Content (% by weight)] Prize division Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 A phase Borage tree extract 10.0 - - - - - Borage tree fermented extract - 10.0 - - - - Borage tree resin extract - - 10.0 - - - Borage tree resin fermentation extract - - - 10.0 - - MCT oil - - - - 10.0 - Camellia oil - - - - - 10.0 Tricetester-4 phosphate 2.0 2.0 2.0 2.0 2.0 2.0 Phage-4 polyglyceryl-2 stearate 7.0 7.0 7.0 7.0 7.0 7.0 Polysorbate 80 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl stearyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 Panthenol 1.0 1.0 1.0 1.0 1.0 1.0 Octyldodecanol 5.0 5.0 5.0 5.0 5.0 5.0 Isopropyl isostearate 5.0 5.0 5.0 5.0 5.0 5.0 Non-sabo roll 0.5 0.5 0.5 0.5 0.5 0.5 B phase kaoline 3.0 3.0 3.0 3.0 3.0 3.0 Carbomer 0.5 0.5 0.5 0.5 0.5 0.5 C phase Sodium hydroxide 1.0 1.0 1.0 1.0 1.0 1.0 Sodium cocoyl glutamate 5.0 5.0 5.0 5.0 5.0 5.0 Alantouille 0.2 0.2 0.2 0.2 0.2 0.2 D phase Pyrrothonol amine 0.2 0.2 0.2 0.2 0.2 0.2 E phase Propylene glycol 15.0 15.0 15.0 15.0 15.0 15.0 Aloe vera leaf juice 2.0 2.0 2.0 2.0 2.0 2.0 Diazolidinyl urea Balance Balance Balance Balance Balance Balance Incense, preservative a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount

First, 30 persons selected from among 20- to 40-year-old women were measured for skin before use with a spectrophotometer (Spectrophotometer CR-2600D, Konica Minolta), and then the skin of Examples 1 to 4 and Comparative Examples 1 to 4 was measured. 2, the skin was re-measured.

The measured values are represented by three average values excluding the maximum value and the minimum value of a *, and the higher the measured value, the more reddish state, and the results are shown in Table 7 below.

[a *, (Arbitrary unit)] division Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Before use 12.46 12.46 12.46 12.46 12.46 12.46 after 2 weeks 11.59 11.48 11.21 11.10 12.38 12.26 After 4 weeks 11.11 11.03 10.89 10.75 12.24 12.21

Referring to Table 7, mask packs (Examples 1 to 4) made of extract of barley water exhibited remarkably reduced erythema of skin after use and excellent skin erythema improvement effect. In particular, it was confirmed that the mask pack (Example 4) containing the fermented extract of bamboo sapwood had the best effect of improving skin erythema. On the other hand, when using mask packs containing no water extract (Comparative Examples 1 and 2), the effect of improving skin erythema was minimal.

EXPERIMENTAL EXAMPLE 7: Evaluation of Skin Heavy Metal Removal Effect

Experiments were conducted to verify the effect of removing the skin heavy metals of the cleansing foams prepared from the water extracts of Examples 1 to 4 according to one embodiment of the present invention. Cleansing foams were prepared as shown in Table 8 below. As a control group of Comparative Example 1 or 2, a group to which a rice bran extract and a seaweed extract, each of which is known to have a fine dust removing function, were respectively applied to the known extracts.

[Content (% by weight)] Prize division Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 A phase Borage tree extract 10.0 - - - - - Borage tree fermented extract - 10.0 - - - - Borage tree resin extract - - 10.0 - - - Borage tree resin fermentation extract - - - 10.0 - - Rice bran extract - - - - 10.0 - Seaweed extract - - - - - 10.0 Stearic acid 8.0 8.0 8.0 8.0 8.0 8.0 Myristic acid 12.0 12.0 12.0 12.0 12.0 12.0 Vitamin E acetate 1.0 1.0 1.0 1.0 1.0 1.0 Propylparaben 0.2 0.2 0.2 0.2 0.2 0.2 B phase glycerin 9.0 9.0 9.0 9.0 9.0 9.0 Propylene glycol 15.0 15.0 15.0 15.0 15.0 15.0 Cocamide DEA 7.0 7.0 7.0 7.0 7.0 7.0 Cocamide Profile Betaine 7.0 7.0 7.0 7.0 7.0 7.0 Methyl paraben 0.4 0.4 0.4 0.4 0.4 0.4 Ethylenediaminetetraacetic acid 0.2 0.2 0.2 0.2 0.2 0.2 Purified water Balance Balance Balance Balance Balance Balance C phase Potassium hydroxide 5.0 5.0 5.0 5.0 5.0 5.0 Incense, preservative a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance Balance Balance

First, two samples of pig skin (1 cm x 1 cm) were immersed in 600 ppm lead solution and 600 ppm cadmium solution, respectively, for 1 hour, and then dried at room temperature. Then, cleansing foams (Examples 1 to 4) (Comparative Examples 1 and 2) containing a rice bran extract and a seaweed extract were applied, washed, and the residual amount of skin heavy metal was measured. The results are shown in Table 9 below.

[Remaining amount of skin heavy metals (ppm)] division Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Cadmium (Cd) Before cleaning 8,400 8,400 8,400 8,400 8,400 8,400 After cleaning 1,240 1,080 940 820 5,400 6,200 Lead (Pb) Before cleaning 11,500 11,500 11,500 11,500 11,500 11,500 After cleaning 2,500 2,300 2,100 1,840 7,200 9,400

As shown in Table 9, the cleansing foams (Examples 1 to 4) prepared with the extract of barley water showed remarkable reduction in the amount of remaining heavy metals in the skin after cleansing, and thus the fine dust removing effect was excellent. Particularly, it was confirmed that the cleansing foam (Example 4) containing the fermented extract of bamboo sapwood had the most excellent fine dust removing effect. On the other hand, when cleaning was carried out using known cleansing foams (Comparative Examples 1 and 2), the residual amount of heavy metals in the skin did not decrease significantly, and the effect of removing fine dust was insignificant.

EXPERIMENTAL EXAMPLE 8: Evaluation of skin texture improvement effect

Experiments were carried out to verify the effect of improving the skin texture of creams prepared from the extract of water-extract of Examples 1 to 4 according to one embodiment of the present invention, and the cream was prepared as shown in Table 10 below. In Comparative Examples 1 and 2, a group to which aloe vera extract, which is known to have a moisturizing function, was applied to each of purified water and known extracts was introduced as a control group.

[Content (% by weight)] Prize division Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 A phase Borage tree extract 10.0 - - - - - Borage tree fermented extract - 10.0 - - - - Borage tree resin extract - - 10.0 - - - Borage tree resin fermentation extract - - - 10.0 - - Purified water - - - - 10.0 - Aloe vera extract - - - - - 10.0 Disodium EDTA 0.01 0.01 0.01 0.01 0.01 0.01 Paraoxybenzoic acid ester 0.2 0.2 0.2 0.2 0.2 0.2 Clofenine 0.2 0.2 0.2 0.2 0.2 0.2 Hyaluronic acid 5.0 5.0 5.0 5.0 5.0 5.0 1% aqueous solution of xanthan gum 5.0 5.0 5.0 5.0 5.0 5.0 Carboxyvinyl polymer 8.0 8.0 8.0 8.0 8.0 8.0 B phase Squall 4.0 4.0 4.0 4.0 4.0 4.0 Dimethicone 3.0 3.0 3.0 3.0 3.0 3.0 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 Polysorbate 60 2.0 2.0 2.0 2.0 2.0 2.0 Sorbitolate 0.5 0.5 0.5 0.5 0.5 0.5 Sheer butter 3.0 3.0 3.0 3.0 3.0 3.0 Glyceryl stearate 1.0 1.0 1.0 1.0 1.0 1.0 Cetostearyl alcohol Balance Balance Balance Balance Balance Balance Stearic acid Balance Balance Balance Balance Balance Balance Incense, preservative a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount

First, 30 pre-selected women aged 20 to 40 years were subjected to PRIMOS light (field of view 45, flexible 3D measuring, GFMesstechnik GmbH) to measure the skin before use of the test subjects, After using the creams of Comparative Examples 1 and 2, the skin was re-measured.

The measured values are shown as three average values except for the maximum and minimum values of Ra. The higher the measured value, the more wrinkled the skin, and the results are shown in Table 11 below.

[Ra, (Arbitrary unit)] division Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Before use 15.71 15.71 15.71 15.71 15.71 15.71 after 2 weeks 14.46 14.01 13.42 13.11 14.87 14.54 After 4 weeks 12.85 12.59 12.31 12.10 14.64 14.21

Referring to Table 11, the cream prepared with the water extract (Examples 1 to 4) showed a remarkably decreased measured value after use and excellent skin texture improving effect. In particular, it was confirmed that the skin cream improving effect of the cream containing the fermented extract of bamboo blossom resin (Example 4) was the most excellent. On the other hand, the use of creams containing no water extract (Comparative Examples 1 and 2) showed little improvement in skin texture.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (9)

A cosmetic composition for the removal of heavy metals or fine dusts, which comprises an extract of Liliaceae as an active ingredient.
The method according to claim 1,
A cosmetic composition comprising a fermented extract prepared by inoculating a fermentation strain into the extract.
The method according to claim 1,
A cosmetic composition comprising a resin extract of Indian bamboo tree.
The method of claim 3,
A cosmetic composition comprising a fermented extract prepared by inoculating a fermentation strain into the resin extract.
The method according to claim 2 or 4,
The fermentation strain may be selected from the group consisting of Lactobacillus sp. , Monascus sp. , Bifidobacterium sp. , Prevotella sp. , Fusobacterium sp. , And Escherichia coli ( Eubacterium sp. ).
The method according to claim 1,
Wherein the extract is selected from the group consisting of water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3-butylene glycol Gt; cosmetic < / RTI > composition.
The method according to claim 6,
Wherein the alcohol is ethanol at a concentration of 65 to 75% (v / v).
8. The method according to any one of claims 1 to 7,
A cosmetic composition having at least one skin improving effect selected from the group consisting of antioxidant, antiinflammatory, skin elasticity improvement, skin hydration control, collagen increase, skin barrier recovery, skin texture improvement, skin wrinkle improvement, DNA damage inhibition and skin erythema reduction.
8. The method according to any one of claims 1 to 7,
Cosmetics formulated with at least one selected from the group consisting of softening longevity, nutritional lotion, moisture cream, nutritional cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder Composition.