KR102111058B1 - A cosmetic composition for skin moisturizing comprising an extract of plant-derived lactobacillus brevis (ami-1109) femented barley - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a green barley fermented extract using a Lactobacillus brevis (AMI-1109) strain. The cosmetic composition exhibits skin moisturizing and skin barrier enhancement effects.

Description

(A COSMETIC COMPOSITION FOR SKIN MOISTURIZING COMPRISING AN EXTRACT OF PLANT-DERIVED LACTOBACILLUS BREVIS (AMI-1109) FEMENTED BARLEY)

The present invention relates to a cosmetic composition comprising a green barley ferment extract extracted using Lactobacillus brevis AMI-1109 strain. The cosmetic composition may exhibit skin moisturizing and skin barrier strengthening effects.

The skin is a membrane that covers the outside of the body and performs various physiological functions to protect the body from environmental factors such as various external stimuli, obstacles, and dryness, and protects and regulates internal organs and other internal organs. do.

However, as the age of the skin increases, the physiological function of the living body decreases, secretion of various hormones that regulate metabolism decreases, and the function of immune cells and the activity of cells decrease. In addition, the appearance or function of the skin changes due to repeated exposure to light or heat, which causes free radicals and free radicals to increase, resulting in excessive physical and chemical irritation and stress. It damages the skin by promoting the creation of freckles and skin aging.

In order to prevent skin aging and damage and maintain a healthier and more beautiful skin, by using physiologically active substances obtained from various animals, plants, microorganisms, etc., in addition to cosmetics, it maintains the skin's unique functions and activates skin cells to rejuvenate the skin Efforts to maintain skin health by promoting metabolism continue, and cosmetics and external preparations for the skin have been developed accordingly.

However, these substances have limitations in the amount of use due to safety problems such as irritation, erythema, and redness when applied to the skin, and it is difficult to expect an effect of improving skin function substantially due to insignificant effects. Therefore, there is a need to develop a new composition for external application for skin that is safer and more effective in living body than an existing composition for external application for skin.

Korean Patent Publication No. 10-2016-0081211

An object of the present invention is to provide a cosmetic composition comprising a fermented extract of green barley.

One aspect of the present invention for achieving the above object relates to a cosmetic composition comprising a green barley fermentation extract.

In the present invention, "green barley" is a kind of barley ( Hordeum vulgare L.) , and is called blue-green foot barley or green wheat belonging to the monocotyledonous potted plant. Green barley contains various vitamins, minerals, and amino acids necessary for the human body, and is known as a nutritional report. According to the results of previous research, 18 amino acids, 15 vitamins, 8 minerals, chlorophyll, as well as superoxide dismutase (SOD) It is known to contain a large amount of components related to endurance, such as hexacosanol, octacosanol, and polycosanol.

In the present invention, "fermentation" refers to a process of decomposing organic substances using enzymes owned by a fermentation strain. It is known that the raw material that has undergone the fermentation process through the fermentation strain increases the effect of at least 2 to up to several tens of times than before fermentation. The fermented metabolites contain various amino acids, organic acids, and antioxidants that are good for the skin to promote skin metabolism and smooth skin texture. In addition, through the fermentation process, the particles become small and the toxicity of heavy metals and the like decomposes, so that the absorption rate is good, and skin troubles and allergic side effects are alleviated.

Accordingly, in the present invention, a fermentation extract was prepared through a fermentation process on green barley, and the present invention intends to provide an excellent cosmetic composition by maximizing the effect of the components contained in green barley.

The green barley ferment extract may be prepared by sterilizing green barley, inoculating the fermentation strain, fermenting under a predetermined condition, and then extracting with a specific solvent.

Specifically, the fermentation extract can be obtained by inoculating a strain of Lactobacillus genus.

More specifically, the strain of genus Lactobacillus may be Lactobacillus brevis AMI-1109.

"Lactobacillus brevis AMI-1109 (KCCM12385P)" of the present invention is a novel strain of Lactobacillus brevis isolated and identified from Kimchi, Jeju Island. For the Lactobacillus brevis strain, reference may be made to Korean Patent Registration No. 10-1968242.

In one embodiment of the present invention, after drying and grinding the green barley, it is mixed with purified water and sterilized by heating in a temperature range of 120 to 125 ° C. After inoculating the sterilized green barley with Lactobacillus brevis AMI-1109 strain, fermented green barley extract was obtained by fermenting for 3-10 days at a temperature range of 30-37 ° C (Example 1).

In addition, specifically, the cosmetic composition containing the green barley ferment extract may be for moisturizing the skin or strengthening the skin barrier.

In the present invention, "moisturizing" includes both supplementing and supplying moisture to the skin, or protecting the skin from moisture. In general, the moisture content of the skin is about 70% in the dermal layer, but decreases toward the epidermal layer, resulting in about 10 to 30% in the stratum corneum. When the moisture content of the cell layer is less than 10%, the skin becomes rough and the body protection function is weakened, resulting in aging. Moisture supply and moisturization are important for skin protection.

In the present invention, the "skin barrier" skin structure is divided into the epidermis exposed on the outside and the dermis on the inside, which is the part corresponding to the outermost stratum corneum layer in the epidermis. The role of the skin barrier is to protect the internal moisture from escaping while preventing external harmful elements from entering the skin.

In the present invention, “strengthening the skin barrier” means that when the skin barrier becomes weak, it is vulnerable to external stimuli and easily loses moisture, thereby increasing the likelihood of skin becoming dry and sensitive, preventing, preventing or improving it.

Specifically, the cosmetic composition comprising the fermented barley barley extract may increase the amount of hyaluronic acid, aquaporin3, AQP3, and filamentgrin.

In an embodiment of the present invention, the effect of increasing the production of hyaluronic acid (Hyaluronic Acid) and aquaporin3, which are skin moisturizing factors, was confirmed, and in particular, the green barley fermentation extract obtained using the Lactobacillus brevis AMI-1109 strain fermented It was confirmed that it exhibits an excellent skin moisturizing effect compared to the unexcited green barley extract (FIGS. 1 and 2).

In one embodiment of the present invention, it was confirmed that the production amount of pilar green, a skin barrier strengthening factor, was increased, and it was confirmed that the green barley fermented extract of the present invention can be used for skin barrier strengthening purposes (FIG. 3).

Accordingly, the green barley ferment extract of the present invention is manufactured using the Lactobacillus brevis AMI-1109 strain, one of the new strains of the genus Lactobacillus, thereby significantly increasing the production of factors related to skin moisturizing and skin barrier strengthening, It can be used as a cosmetic composition for skin moisturizing and skin barrier strengthening or as a skin external agent.

The cosmetic composition of the present invention is a solution, ointment for external use, cream, foam, nutrient lotion, soft lotion, pack, softener, latex, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension, emulsion Liquids, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays can be prepared in a formulation selected from the group consisting of, but not limited to no.

The cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and for example, oil, water, surfactant, moisturizer, lower alcohol, and thickener as common ingredients , Chelating agents, pigments, preservatives, fragrances, etc. may be appropriately blended, but is not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. as carrier components This may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component, and in the case of a spray, additionally, chlorofluorohard Propellants such as locarbon, propane / butane or dimethyl ether, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, and the like can be used, in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspensions such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar or trakant may be used, but is not limited thereto. These may be used alone or in combination of two or more.

Specifically, in the cosmetic composition as described above, the green barley ferment extract may be included in an amount of 0.005% to 30.0% by weight based on the total weight of the cosmetic composition. More specifically, it may be included as 0.01% to 20% by weight. Within the above range, there is an advantage of exhibiting an excellent skin whitening effect, and there is an advantage that the formulation of the composition is stabilized.

In addition, the composition of the present invention can be used as a transdermal administration method such as applying or spraying directly on the skin, and the route of administration of the composition of the present invention can be administered through any general route as long as it can reach the target tissue.

The amount of the composition of the present invention can be appropriately adjusted according to the individual difference or formulation such as age, degree of lesions, etc., and applied to the skin once to several times a day to be used for a week to several months.

Cheongbori fermentation extract of the present invention is excellent in skin moisturizing and skin barrier strengthening effect.

In addition, since the composition of the present invention is a substance derived from natural products, it is non-toxic, and does not cause side effects even when applied to the human body, and thus has high utilization as a cosmetic composition molar.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the invention.

1 is a graph showing the comparison results of hyaluronic acid (Hyaluronic Acid) production of green barley fermented extract (Example 1) and green barley extract (Comparative Example 1).
Figure 2 is a graph showing the comparison results of the production of aquaporin 3 (Aquaporin3, AQP3) of the green barley fermented extract (Example 1) and green barley extract (Comparative Example 1).
Figure 3 is a graph showing the results of comparison of the amount produced by Filaggrin of the green barley fermented extract (Example 1) and the green barley extract (Comparative Example 1).

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, but it is apparent that the present invention is not limited by the following examples.

Example 1.Lactobacillus brevis ( Lactobacillus brevis  AMI-1109) Preparation of Green Barley Ferment Extract

The washed and dried blue barley outpost was coarsely crushed with purified water, and high pressure hot water was extracted for 15 minutes at 121 ° C with 20 times the extraction solvent (purified water). Inoculum for the fermentation was sterilized lactic acid bacteria culture medium (Lactobacillus MRS Broth, deep nose, the United States of America), the lactic acid bacteria (Lactobacillus brevis AMI-1109) was used to recover only the microbial cells after the culture is completed cheongbori to 1x10 ⁷ cfu / ml concentration incubated at The extract was inoculated. Fermentation was conducted using a fermentation tank for 1 to 3 days, maintained at 25 to 35 ° C. The fermented green barley fermented product was centrifuged to remove the cells, and finally filtered using 0.2 μm filter paper. The extracted filtered barley barley extract was used by lyophilization.

Comparative Example 1. Preparation of green barley extract

The washed and dried blue barley outpost was coarsely crushed with purified water, and high pressure hot water was extracted for 15 minutes at 121 ° C with 20 times the extraction solvent (purified water). The extracted green barley extract was centrifuged to recover the extract supernatant, and the extract was filtered through a filter having a size of 0.2 μm to prepare a green barley extract. The extracted filtered barley barley extract was used by lyophilization.

Experimental Example 1. Culture of keratinocytes

The skin keratinocytes, HaCaT cells, were 37 ° C. using Dulbecco's Modified Eagle's Medium (DMEM) medium containing 100 units / ml penicillin-streptomycin and 10% fetal bovine serum (FBS) from CG Hyun (Jeju National University, Korea). Cultured in a 5% CO ₂ incubator, passage was performed at intervals of 3 to 4 days.

Experimental Example 2. Cytotoxicity evaluation (EZ-cytox assay)

To confirm the skin stability of Example 1, cytotoxicity evaluation was performed using the EZ-cytox assay method. The EZ-cytox assay is a representative method of measuring cell viability using the principle of generating orange water-soluble formazan by reacting with dehydrogenase of living cells.

Specifically, in order to confirm the toxicity in keratinocytes, HaCaT cells were dispensed into 96-well plates at 5 X 10 ⁴ cells / mL using DMEM medium with 10% FBS added, and 37 ° C, 5% CO ₂ conditions After reacting for 18 hours, the absorbance was measured at 450 nm using a microplate reader. Average absorbance values were obtained for each sample group, and cell viability was evaluated by comparing with the absorbance values of the control group. Table 1 shows the results.

Cell viability (% control) Concentration (ug / ml) 0 50 100 200 Example 1 100 117 120 121

As shown in Table 1, the green barley ferment extract of Example 1 exhibited excellent cell viability regardless of concentration, and it was confirmed that stability was high because there was little toxicity to keratinocytes.

Experimental Example 3. Confirmation of moisturizing effect of green barley ferment extract

3-1. Confirm the effect of increasing the production of hyaluronic acid (Hyaluronic Acid)

In order to confirm the moisturizing effect of Example 1 and Comparative Example 1, an experiment was performed to confirm the effect of increasing the amount of hyaluronic acid production.

Specifically, HaCaT cells were dispensed at 1.0 × 10 ⁵ cells / mL in a 24 well plate and cultured for 18 hours at 37 ° C. and 5% CO ₂ conditions. The cells were cultured for 24 hours by exchanging with serum-free DMEM and treating Example 1 and Comparative Example 1. After that, the culture medium was collected, centrifuged at 15,000 rpm for 5 minutes, and the supernatant was removed and stored (-20 ° C) until quantification. The enzyme immunoassay (ELISA: Enzyme-Linked Immunosorbent Assay) was used as a hyaluronic acid ELISA kit (Elabscience Biotechnology Co., Ltd) and was performed by the method provided by the manufacturer. Table 2 shows the results.

Hyaluronic acid production increase (%) - Concentration (μg / ml) % % of control - - 100 0.64 Green barley 50 80 0.10 Green barley fermentation 50 154.83 0.54

As shown in Table 2, it was confirmed that the green barley ferment extract of Example 1 significantly increased the amount of hyaluronic acid production compared to the green barley extract of Comparative Example 1, and the superiority of the green barley ferment extract using the Lactobacillus Brevis AMI-1109 strain The skin moisturizing effect was confirmed. .

3-2. Confirmation of the increase effect of the moisturizing factor Aquaporin 3 (AQP3)

In order to confirm the moisturizing effect of Example 1 and Comparative Example 1, an experiment was performed to confirm the effect of increasing the amount of aquaporin 3 production.

HaCaT cells were dispensed into 24 well plates at 1.0 × 10 ⁵ cells / mL and cultured for 18 hours at 37 ° C. and 5% CO ₂ conditions. The cells were exchanged for serum-free DMEM, and the samples were processed to incubate for 24 hours. Subsequently, each group was treated with PBS containing no drug that could affect protein quantification after removal of the culture solution and washing with PBS. The cells were lysed by repeating incubation at low temperature and room temperature, and then the protein was collected. Quantitatively, AQP3-ELISA kit (Elabscience Biotechnology Co., Ltd) was used and was performed by the method provided by the manufacturer. Table 3 shows the results.

Aquaporin 3 (AQP3) production increase (%) - Concentration (μg / ml) % % of control - - 100 0.76 Green barley 50 59.60 0.45 Green barley fermentation 50 200.85 0.31

As shown in Table 3, it was confirmed that Example 1 significantly increased the production amount of the aquaporin 3 factor compared to Comparative Example 1, thereby confirming that it was effective in moisturizing the skin by increasing the production of the aquaporin 3 moisturizing factor. Did.

Experimental Example 4. Confirmation of skin barrier strengthening effect of green barley fermented extract

In order to confirm the skin barrier strengthening effect of Example 1 and Comparative Example 1, an experiment was performed to confirm the effect of increasing the amount of Filaggrin production.

HaCaT cells were dispensed into 24 well plates at 1.0 × 10 ⁵ cells / mL and cultured for 18 hours at 37 ° C. and 5% CO ₂ conditions. The cells were exchanged for serum-free DMEM, and the samples were processed to incubate for 24 hours. Subsequently, each group was treated with PBS containing no drug that could affect protein quantification after removal of the culture solution and washing with PBS. The cells were lysed by repeating low temperature and room temperature incubation to collect the protein. Quantitatively, a Filaggrin-ELISA kit (Elabscience Biotechnology Co., Ltd.) was used and was performed by the method provided by the manufacturer. Table 4 shows the results.

Increased amount of pilar green (FLG) production (%) - Concentration (μg / ml) % % of control - - 100 0.48 Green barley 50 130.40 0.55 Green barley fermentation 50 152.29 0.69

As shown in Table 4, it was confirmed that Example 1 increased the amount of the production of a good pilargreen factor compared to Comparative Example 1, and it was confirmed that the green barley ferment extract of the present invention can be used for skin barrier strengthening use. .

Preparation Example 1 Preparation of Essence Containing Fermented Green Barley Extract

A cosmetic composition of an essence formulation was prepared as shown in Table 5 below (unit:% by weight).

Raw material name Content (% by weight) Cetearyl alcohol 2 Cetearyl glucoside One Jojoba seed oil 5 Silica 0.5 glycerin 10 Green barley ferment extract 5 Purified water to 100

Preparation Example 2. Preparation of cream containing fermented barley extract

A cosmetic composition of a cream formulation was prepared as shown in Table 6 below (unit: wt%).

Composition Content (% by weight) Green barley ferment extract 5 Stearic acid 15 ethanol One Potassium hydroxide 0.7 glycerin 5 Propylene glycol 3 antiseptic Proper incense Proper Purified water to 100

The foregoing description of the present invention is for illustration only, and a person having ordinary knowledge in the technical field to which the present invention pertains can understand that it can be easily modified to other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all modifications or variations derived from the meaning and scope of the claims and equivalent concepts should be interpreted to be included in the scope of the present invention.

Korea Microbial Conservation Center (Overseas) KCCM12385P 20181112

Claims (6)

As a cosmetic composition for moisturizing the skin comprising a green barley fermented extract,
The fermented extract is obtained by inoculating a strain of Lactobacillus genus,
The Lactobacillus ( Lactobacillus ) genus strain is Lactobacillus Brevis AMI-1109 ( Lactobacillus brevis AMI-1109) KCCM12385P, cosmetic composition. delete delete According to claim 1,
The cosmetic composition is to further include a skin barrier strengthening, cosmetic composition. The method of claim 4,
The cosmetic composition is hyaluronic acid (Hyaluronic Acid), aquaporin 3 (Aquaporin3, AQP3), and characterized in that to increase the amount of production of any one or more of the elements of Filaggrin (Filaggrin), cosmetic composition. The method of claim 1 or 4,
The cheongbori fermentation extract is 0.001% to 30.0% by weight based on the total weight of the cosmetic composition by dry weight, cosmetic composition.

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