JP2004137196A - Emulsifying agent and cosmetic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain an emulsifying agent which has excellently emulsifying action and is highly safe to organisms due to low toxicity and low skin irritation, or the like, has excellent adhesivity to the skin and sense of use and is especially suitable for emulsifying a cosmetic and a medicine for external use, and the cosmetic and the medicine for external use formulated with the emulsifying agent. <P>SOLUTION: The emulsifying agent is obtained by using a lactic fermentation product prepared by fermenting one or more kinds selected from various cereals with lactic acid bacteria. The cosmetic or the medicine for external use is obtained by formulating a cosmetic or a medicine for external use with the lactic fermentation product. <P>COPYRIGHT: (C)2004,JPO

Description

【００９１】
即ち、成分４を成分３に溶解し、これに成分２及び成分５を加えて混合し、８０℃に加熱した。これを、成分１の油性成分を８０℃に加熱したものに加え、５０００ｒｐｍ×２分間の条件でホモジナイズした後、攪拌下に水冷して室温まで冷却した。ここに得られた乳化物をそれぞれ５０ｍｌのスクリュウ瓶に入れ、調製直後の乳化状態と、室温又は５０℃に３カ月間保存した時の乳化状態の経時変化を目視観察し、以下の基準により評価した。
◎　：良好
○　：１カ月以上経過後に極く僅かに分離が認められる
△　：２週間〜１カ月経過後に僅かに分離が認められる
×　：１週間以内に完全分離する
なお、比較試料のハト麦微粉末、アワ微粉末及び大豆微粉末の場合には、いずれも乳化物調製直後に油浮きが認められ、乳化状態は不良であった。
【００９２】
［結果］
結果を表２に示す。
【表２】

【００９３】
表２に示す通り、本発明の雑穀類の乳酸菌醗酵物からなる乳化剤は、いずれもすぐれた乳化作用を有し、長期間安定な乳化物を与える。これに対して、乳酸菌醗酵を行っていない雑穀類の微粉末によっては、安定な乳化物は得られない。
【００９４】
試験例２．乳化作用（その２）
乳酸菌醗酵物に増粘剤を併用した場合の乳化安定性への影響を調べた。
［試験方法］
表３に示す成分組成（単位：部）からなる３種の乳化物を調製し、それらの経時変化を観察した。
【００９５】
【表３】

【００９６】
即ち、成分４を成分３に溶解し、これに成分５のいずれかを加えて混合した。別に、成分２と６の混合物及び成分１をそれぞれ８０℃に加温後、成分１に成分２と６の混合物を加え、ヒスコトロン（５０００ｒｐｍ）で２分間ホモジナイズした。これに上記の成分３、４、５の混合物を加え、ヒスコトロン（５０００ｒｐｍ）でさらに１分間ホモジナイズした後、攪拌しながら室温まで水冷した。ここに得られた乳化物をそれぞれ５０ｍｌのスクリュウ瓶に入れ、調製直後の乳化状態と、室温又は５０℃に３カ月間保存した時の乳化状態の経時変化を目視観察し、試験例１と同様の基準に従って評価した。
【００９７】
［結果］
結果を表４に示す。
【表４】

【００９８】
表４の結果から、乳酸菌醗酵物に増粘剤を併用した場合、得られる乳化物の安定性が一層向上することが判る。
【００９９】
試験例３．皮膚刺激性
本発明の乳酸菌醗酵物の皮膚刺激性を、化粧料の乳化剤として汎用されている成分のそれと比較した。
［試料］
下記の成分を、各々日局親水ワセリンに５％の濃度となるように練合したものを試料として用いた。
（１）実施例１の乳化剤（本発明試料）
（２）ポリオキシエチレン（２０）ソルビタンモノオレエート（比較試料ａ）
（３）親油型モノステアリン酸グリセリン（比較試料ｂ）
【０１００】
［試験方法］
年齢２０〜５０歳の成人男子５名を被験者とし、各々の上腕部内側をエタノールで拭って皮脂を除去し、該部位に、フィンチャンバーのアルミ板に各々の試料０．２ｇを塗布したものを貼付した。２４時間後にフィンチャンバーを除去し、皮膚刺激の程度をつぎに述べる方法並びに基準により判定した。
【０１０１】
［判定］
パッチ除去後１時間後、２４時間後及び４８時間後に、貼付部位の紅斑及び浮腫の状況を、以下の「ドレイズ法による皮膚刺激性判定基準」に基づき目視判定し、被験者５名の平均値を求めた。
（紅斑）
スコア　　　皮膚の状態
０　　：　紅斑なし
１　　：　極く軽度の紅斑
２　　：　明らかな紅斑
３　　：　中程度から強い紅斑
４　　：　深紅色の強い紅斑に軽い痂皮形成
（浮腫）
スコア　　　皮膚の状態
０　　：　浮腫なし
１　　：　極く軽度の浮腫
２　　：　明らかな浮腫（周囲と明らかに区別可能）
３　　：　中程度の浮腫（１ｍｍ以上の盛り上がり）
４　　：　強い浮腫（さらに周囲にも広がり）
【０１０２】
［結果］
結果を表５に示す。
【表５】

【０１０３】
本試験で比較試料として用いたａ、ｂの活性剤は、比較的安全性が高いところから、化粧料の乳化に汎用されているものであるが、表５の結果から明らかな通り、本発明の乳酸菌醗酵物からなる乳化剤は、それらの乳化剤よりもさらに皮膚刺激性が少なく、安全性に極めてすぐれている。
【０１０４】
試験例４．モニターテスト
下記の各試料について、モニターテストにより使用感と安全性（皮膚刺激性）を評価した。
［試料］
（１）　処方例２の乳液（本発明乳液）
（２）　処方例２に於いて、実施例１の乳化剤５．０部に代えてポリオキシエチレン（２０）ソルビタンモノオレエート２．０部を用いるほかは処方例２と同様にして得られた乳液（比較乳液）
【０１０５】
［試験方法］
無作為に抽出した年齢１８〜５０歳の女性２０名を被験者とし、各乳液を１日２回（朝、晩）、５日間左上腕部にそれぞれ塗布した時の使用感及び皮膚刺激性を、下記の各項目について評価した。
（使用感）
イ．手に取った感触
ロ．塗布時の伸び
ハ．塗布時のなめらかさ
ニ．浸透性（浸透感）
ホ．塗布後の感触
（皮膚刺激性）
ヘ．塗布時の刺激
ト．塗布後の刺激
評価は、使用感については５段階評価（Ａ：良い、Ｂ：やや良い、Ｃ：普通、Ｄ：やや悪い、Ｅ：悪い）によって、又皮膚刺激性については３段階評価（Ａ：刺激なし、Ｂ：違和感あり、Ｃ：刺激あり）によってそれぞれ行った。
【０１０６】
［結果］
結果を表６及び表７に示す。
【表６】

【０１０７】
【表７】

【０１０８】
表６及び表７に示す通り、本発明の乳酸菌醗酵物からなる乳化剤を使用して調製された乳液は、使用感に於いて高い評価を得ており、又皮膚刺激性も認められなかった。
これに対して、合成界面活性剤を用いて得られた比較乳液は、使用感に於いて劣るだけでなく、若干の皮膚刺激性も認められた。
【発明の効果】
雑穀類の乳酸菌醗酵物からなる本発明の乳化剤は、良好な乳化力を具えると共に、低毒性でかつ皮膚刺激が極めて少ないなど人に対する安全性にすぐれ、化粧料、外用医薬品、食品など広汎な分野に於ける乳化物の調製に用いて有用である。　なかでも、化粧料や外用医薬品の乳化剤として用いた場合には、該乳酸菌醗酵物の有する高い保湿・保水能と皮膚に対する密着性の故に、得られる化粧料、外用医薬品は、皮膚に対する感触、使用感にすぐれたものとなる。
従って、本発明によれば又、上記の乳酸菌醗酵物を含んでなり、乳化安定性と生体安全性、さらには使用感の良好な化粧料及び外用医薬品が提供される。[0001]
TECHNICAL FIELD OF THE INVENTION
INDUSTRIAL APPLICABILITY The present invention has a good emulsifying action, and is excellent in safety such as low toxicity and little skin irritation, and is used for preparation of emulsions in a wide range of commercial fields such as cosmetics and external medicines, and foods. The present invention relates to a suitable emulsifier and cosmetics and external medicines containing or using the same.
[0002]
[Prior art]
Synthetic surfactants such as polyoxyethylene alkyl ethers and sorbitan fatty acid partial esters are mainly used for emulsification of cosmetics and external medicines.
However, in the case of synthetic surfactants, irritation to the skin is inevitable to a greater or lesser degree.Therefore, it is possible to reduce the amount of active agents used as much as possible or to emulsify without using them in some cases. Although attempts have been made, there are many cases where problems occur in terms of uniformity of emulsification and stability over time, and satisfactory results have not always been obtained.
[0003]
[Problems to be solved by the invention]
On the other hand, it has been proposed to prepare a highly safe emulsion for the skin by using components derived from natural products such as saponin, lecithin and stevioside as an emulsifier, and some of them have been practically used. There are aspects that are not always sufficient in terms of stability, use feeling when applied to the skin, ease of production, and the like, and further improvement is required.
In order to solve the problems of the prior art, the present inventors have found that lactic acid bacteria-fermented rice obtained by first fermenting rice with lactic acid bacteria has excellent emulsifying action, emulsifying stabilizing action and high biosafety, Lactic acid bacteria fermentation products of cereals as a result of research and exploration to find out what could become an emulsifier in components derived from natural products. Similarly, they have good emulsifying action and biosafety, and have learned that they can be used as emulsifiers in a wide variety of fields such as cosmetics, external medicines, and foods, thus completing the present invention.
[0004]
[Means for Solving the Problems]
That is, the present invention relates to an emulsifier comprising a fermented product of lactic acid bacteria obtained by fermenting cereals with lactic acid bacteria, and a cosmetic or topical medicine containing the fermented product of lactic acid bacteria.
In addition, in the present invention, the cosmetic is used in a sense that it includes quasi-drugs in addition to so-called cosmetics.
[0005]
BEST MODE FOR CARRYING OUT THE INVENTION
Hereinafter, the present invention will be described in detail.
Examples of the cereals used as the fermented product of the lactic acid bacteria fermentation in the present invention include barley, wheat, oats, barley, oats, oats, rye, and so on, soybeans, black soybeans, red beans, green beans, hand-held beans. , Soybeans, quail beans, peas, peas, lentils, white flowers, red flowers, tiger beans, green beans, chickpeas, broad beans, tree beans, etc., millet, millet, millet, buckwheat, amaranth, corn, etc. And the like.
Among them, from the viewpoint of the emulsifying ability of the fermented lactic acid bacteria or the stability of the raw material quality, etc., barley, wheat and pigeon barley, soybeans and red beans as beans, millet, millet, millet, Millet and corn are preferred, especially barley, red beans, millet and millet are most preferred.
In the present invention, germinated products of the above-mentioned cereals, such as germinated soybeans, germinated barley, etc., can also be used as fermented products. Fermented products can be obtained.
In preparing the fermented product of lactic acid bacteria of the present invention, it is general to use any one of the seeds of the above-described cereals, but in some cases, a mixture of two or more seeds may be used. .
[0006]
Examples of lactic acid bacteria used for fermentation of cereals include Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, Lactobacillus cellobiosas, and Lactobacillus cellobiosas. There are L. vacinostercus, Streptococcus faecalis, Bacillus coagulans, and the like. From the viewpoint of the emulsifying ability of the resulting lactic acid bacteria fermented product, Lactobacillus sp. (Str Use of ptococcus faecalis) are particularly preferred.
[0007]
Fermentation of cereals by these lactic acid bacteria is performed, for example, as follows.
First, seeds of cereals are subjected to appropriate means such as washing with water and ethanol treatment to remove germs which hinder lactic acid bacteria fermentation. The seeds are immersed in 1 to 5 times the amount of purified water, and 1 to 4% by weight of saccharide and 10⁷-10⁸/ Ml, and fermentation is performed under anaerobic conditions at about the optimal fermentation temperature of the lactic acid bacteria used for 2 to 10 days. As the saccharide, glucose, fructose, galactose, sucrose and the like are used, and among them, fructose is most preferable. Moreover, yeast extract, malt extract, etc. may be used instead of saccharide.
[0008]
The liquid containing the fermented lactic acid bacteria of cereals obtained by the above fermentation step may be subjected to the milling step as it is, concentrated if necessary, and blended into a cosmetic or the like. The lactic acid bacteria fermented product is separated from the fermentation broth, washed with water, adjusted for water if necessary, then pulverized using a suitable pulverizing means such as mixer pulverization, roll pulverization, air pulverization, and, if necessary, drying treatment. Furthermore, the mixture is sieved and provided as an emulsifier for cosmetics and the like.
[0009]
When the emulsifier of the present invention comprising a lactic acid bacteria fermented product of cereals obtained as described above is blended or applied to cosmetics or the like, the fermented product alone has sufficient emulsifying power, and the obtained emulsified product is Although the emulsion stability is satisfactory enough for practical use, the stability of the emulsion can be further improved by further using a thickener in combination with the fermented product.
[0010]
As the thickener, those commonly used in cosmetics and the like can be used. Specifically, for example, components derived from brown algae, green algae or red algae such as alginic acid, agar, carrageenan, fucoidan; pectin; Polysaccharides such as locust bean gum; gums such as xanthan gum, tragacanth gum, and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; and high synthetic levels such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, and acrylic acid / methacrylic acid copolymer. Molecules; hyaluronic acid or a derivative thereof, polyglutamic acid or a derivative thereof, and the like.
Among them, from the viewpoint of emulsification stability, and further from the viewpoint of not impairing the high biosafety characteristic of the fermented product of lactic acid bacteria of the present invention, in particular, hyaluronic acid or a derivative thereof, polyglutamic acid or a derivative thereof, xanthan gum or tragacanth gum It is also preferable to use components derived from brown algae, green algae or red algae such as alginic acid, agar, carrageenan, fucoidan and the like.
[0011]
When these thickeners are used in combination, the amount used varies depending on the type of thickener used, but generally ranges from 1 to 100 parts by weight of solids per 100 parts by weight of solids of lactic acid bacteria fermented product. It is preferably in the range of 5 to 50 parts by weight, and from such a range, an optimum one is selected in consideration of the use of the emulsion, required performance, and the like.
[0012]
When used in combination with the lactic acid bacteria fermentation product, the thickener may be used by mixing it with the lactic acid bacteria fermentation product in a predetermined ratio in advance, or when emulsifying cosmetics or the like using the lactic acid bacteria fermentation product, It may be added to the emulsification system before or after the addition, or simultaneously with the fermented product. In some cases, it can be added after emulsification.
[0013]
The emulsifier comprising the fermented product of cereals of the cereals of the present invention is provided with a good emulsifying action, provides an emulsion with high stability, and is excellent in safety to the human body such as low toxicity and extremely little irritation to the skin, It is useful for preparing emulsions in a wide variety of fields such as cosmetics and external medicines, as well as foods and agricultural chemicals.
Above all, when used as an emulsifier for cosmetics or external medicines, the resulting lactobacillus fermented product has an extremely high texture and feel on the skin due to the high water retention / moisture retention and adhesiveness to the skin. There is also the advantage of being good.
Therefore, according to the present invention, there are provided cosmetics and external medicines containing the fermented lactic acid bacteria of the above-mentioned cereals, and having excellent emulsification stability and biosafety as well as excellent feeling in use.
[0014]
Examples of the cosmetics of the present invention comprising a fermented product of lactic acid bacteria of cereals include basic cosmetics such as milky lotions, creams, lotions, essences, and packs; makeup cosmetics such as lipsticks, foundations, liquid foundations, and makeup press powders Cleansing cosmetics such as facial cleansers, shampoos, and rinses; hair cosmetics such as hair treatments, conditioners, hair creams, hair dyes, and hair styling products; oral cosmetics such as toothpastes and mouthwashes; Foreign goods and the like.
In addition, examples of the external medicine of the present invention comprising a fermented product of lactic acid bacteria of cereals include creams, emulsions, and lotions.
[0015]
The amount of the fermented lactic acid bacteria of the cereals of the present invention when blended in cosmetics varies depending on the type of cosmetics and the like.For example, in the case of basic cosmetics, makeup cosmetics, hair cosmetics and quasi-drugs, If present, it is generally in the range of 0.5 to 20% by weight (as solid content of lactic acid bacteria fermented product; the same applies hereinafter), preferably in the range of 2 to 10% by weight. It is preferably in the range of 5 to 20% by weight, and in the case of oral cosmetics generally in the range of 0.5 to 20% by weight, preferably 2 to 10% by weight.
The amount of the compound to be applied to an external medicine is generally in the range of 0.5 to 20% by weight, preferably 2 to 10% by weight in the case of a cream, an emulsion or a lotion.
If a thickener is used in combination when blending the lactic acid bacteria fermentation product, the blending amount of the lactic acid bacteria fermentation product can be reduced to 40 to 80% of that in the case of the above single use.
[0016]
In addition, when the fermented product of lactic acid bacteria of the cereals of the present invention is blended into cosmetics or the like, when the fermented product of lactic acid bacteria is preliminarily mixed with one or more kinds selected from hydrophilic substances, particularly saccharides and glycols, The familiarity and dispersibility of the fermented product of lactic acid bacteria in water is improved, and it is preferred because it can be easily incorporated into cosmetics and the like.
[0017]
In this case, the saccharides include, for example, monosaccharides such as glucose, fructose, arabinose, and galactose; disaccharides such as sucrose, lactose, maltose, and trehalose; oligosaccharides such as fructooligosaccharides, xylo-oligosaccharides, and maltooligosaccharides; Sugar alcohols such as xylitol can be used.
[0018]
Examples of the glycols include glycerin, propylene glycol, 1,3-butylene glycol, and polyethylene glycol.
[0019]
Among these saccharides and / or glycols (hereinafter sometimes referred to as admixture components), use of saccharides is preferred from the viewpoint of the effect of improving the familiarity and dispersibility of the fermented product of lactic acid bacteria with water, and among the saccharides, Most preferred is the use of alcohols. .
[0020]
The effect of improving dispersibility is more effectively exhibited when the admixture of fermented lactic acid bacteria and saccharides and / or glycols is in a wet state with water or a liquid substance. When used, the resulting mixture is brought into a wet state having a moisture content in the range of 10 to 40% by weight, particularly 15 to 30% by weight, for example, by blending them with the lactic acid bacteria fermentation product in the form of an aqueous solution. Is preferred.
On the other hand, when using glycols such as glycerin and 1,3-butylene glycol which are liquid at normal temperature, it is possible to make the admixture wet based on the properties of the glycol itself, and water is not used. Although not necessary, these may be used in some cases.
[0021]
The amount of the saccharide or glycol added to the fermented lactic acid bacteria is generally 5 to 50 parts by weight, preferably 10 to 40 parts by weight, based on 100 parts by weight of the fermented lactic acid bacteria (solids). It is in the range of parts by weight.
[0022]
As a method for preparing a mixture of a fermented product of lactic acid bacteria and sugars and / or glycols, a method in which a predetermined amount of a mixed component is added to the fermented product of lactic acid bacteria and a uniform mixture is produced by using an appropriate mixing means, is commonly used. The method is not particularly limited as long as it is carried out according to the method, but when a solid substance such as a monosaccharide or a sugar alcohol is used at room temperature as an admixture component, the above-described method is used in order to more effectively exhibit the effect of improving the dispersibility of the admixture. As described above, the water content of the lactobacillus fermentation product is adjusted to a desired value by mixing the lactobacillus fermentation product with the lactobacillus fermentation product in the form of an aqueous solution, or, in some cases, mixing the powdered powder and adding water. Is preferred.
[0023]
When blending the mixture obtained as described above into cosmetics, etc., it is already known that as a result of improving the familiarity and dispersibility in water, the blending workability and the homogeneity of the obtained product are significantly improved. As described above, the lactic acid bacteria fermented product of the cereals of the present invention shows acidity when dispersed and dissolved in water due to the characteristics of lactic acid bacteria fermentation. In some cases, a summation process is required.
An alkaline substance may be further added to the mixture in order to eliminate the complexity of the process and to obtain a mixture having improved workability and handleability.
[0024]
In this case, examples of the alkaline substance include hydroxides of alkali metals or alkaline earth metals such as sodium hydroxide, potassium hydroxide, calcium hydroxide and magnesium hydroxide; alkalis of weak acids such as carbonic acid, citric acid and phosphoric acid Metal salts; amines such as ethanolamine, diethanolamine, triethanolamine and isopropanolamine; basic amino acids such as arginine, lysine and histidine; ammonia water, aminomethylpropanol and aminomethylpropanediol.
[0025]
The amount of the alkaline substance varies depending on the kind of the alkaline substance to be used and the like, but is generally such an amount that the pH of a 10% by weight aqueous dispersion or aqueous solution of the composition is in the range of 5.5 to 7.5. It is better to do.
[0026]
When preparing a cosmetic or a topical medicine containing the fermented product of cereals of the cereals of the present invention, as a component thereof, components usually used in cosmetics and the like, for example, oil components, humectants, thickeners, antiseptic, A bactericide, a powder component, an ultraviolet absorber, an antioxidant, a dye, a fragrance, a physiologically active ingredient, a drug, and the like can be appropriately used. If necessary, a surfactant may be used in combination.
[0027]
Here, as the oily component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, Plant-derived fats and oils such as macadamia nut oil and plant-derived squalane; animal-derived fats and oils such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax and lanolin; liquid paraffin, vaseline, paraffin wax, squalane and the like Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, ole Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.
[0028]
Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, and the like, and sugars such as trehalose, lactic acid bacteria-fermented rice, hyaluronic acid, and the like. Derivatives, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, sea buckwheat extract, seaweed extract, seafood-derived collagen and its derivatives, various amino acids and their derivatives.
[0029]
Examples of the thickener include alginic acid, agar, carrageenan, brown alga such as fucoidan, components derived from green algae or red algae, syrup extract, pectin, locust bean gum, polysaccharides such as aloe polysaccharide, xanthan gum, tragacanth gum, guar gum and the like. Gums, cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose, synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives, polyglutamic acid and its derivatives Is mentioned.
[0030]
Examples of preservatives and disinfectants include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophen, hexachlorophen, chlorhexidine hydrochloride, benzal chloride Examples include ruconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodeinyl urea), 1,2-pentanediol, various essential oils, and bark dry distillate.
[0031]
Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, silk powder, cereals (rice, There are powders of wheat, corn, millet, etc.) and powders of legumes (soybeans, red beans, etc.).
[0032]
Examples of the ultraviolet absorbent include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, and 2-hydroxy-4. -Methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .
[0033]
Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives, various polyphenols, sunflower extract, rice extract and the like.
[0034]
Examples of the physiologically active components include, as whitening components, t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, arbutin and its derivatives, ellagic acid and its derivatives, resorcinol derivatives, soybean extract, and saxifrage extract , Rice bran extract, hydrolyzate of rice bran extract, hydrolysed extract of white mustard, seagrass extract, seaweed extract such as kelp, seaweed extract such as eelgrass, linoleic acid and derivatives or processed products thereof (for example, Liposomal linoleic acid, etc.), 2,5-dihydroxybenzoic acid derivatives, etc. are animal or fish-derived collagen, nicotinic acid and its derivatives, glycyrrhizic acid and its derivatives (dipotassium salt, etc.) as skin aging prevention and skin roughness improving components. , T-cycloamino acid derivatives, vitamin A and derivatives thereof Vitamin E and its derivatives, allantoin, α-hydroxy acids, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, barley extract, chamomile extract, carrot extract, aloe extract and other crude drug extract extracts, There are a hydrolyzate of rice extract, hydrolyzate of rice bran extract, fermented rice extract, extract of honeywort, extracts of seaweed such as eelgrass, and soybean extract.
[0035]
Examples of the above kojic acid derivatives include kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid esters such as kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, examples of ascorbic acid derivatives include sodium L-ascorbic acid-2-phosphate, magnesium L-ascorbic acid-2-phosphate, sodium L-ascorbic acid-2-sulfate, and L-ascorbic acid-2. -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α -D-glucopyranosyl-L-ascorbin Ascorbic acid sugar derivatives such as acid), acylated 6-positions of these ascorbic acid sugar derivatives (acyl group is hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetraester L-ascorbic acid tetrafatty acid esters such as lauric acid ester and the like, resorcinol derivatives such as 4-n-butyl resorcinol and 4-isoamyl resorcinol, and 2,5-dihydroxybenzoic acid derivative such as 2,2 5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc., as nicotinic acid derivatives, for example, nicotinamide, benzyl nicotinate, etc., as vitamin E derivatives Is, for example, Glutamic E nicotinate, vitamin E linoleate and the like, as the α- hydroxy acids such as lactic acid, malic acid, succinic acid, citric acid, α- hydroxy octanoic acid.
[0036]
Drugs include anti-inflammatory and analgesic agents such as ethyl aminobenzoate, indomethacin, sodium heparin, and methyl salicylate; anti-inflammatory agents for corticosteroids such as hydrocortisone, dexamethasone, fluocinolone acetonide, and prednisolone; benzalkonium chloride, chloride Fungicides such as benzethonium, chlorhexidine, and sulfadiazine; antibiotic fungicides such as erythromycin, tetracycline, chloramphenicol, and tricomycin; antifungal agents such as clotrimazole, tolnaftate, miconazole nitrate, ketoconazole; diphenhydramine, chlorpheniramine maleate , Isohistendyl hydrochloride, clemastine fumarate and other antihistamines; urea, salicylic acid, vitamin A, vitamin E, vitamin C, vitamin B₆Skin roughness improving agents, etc.
[0037]
As described above, it is not always necessary to use the surfactant in combination, but if it is used in combination, the surfactant having excellent biosafety such as sucrose fatty acid ester, lecithin and its derivative, and enzyme-treated stevia is used. Is preferably used in a small amount.
[0038]
Next, the present invention will be described more specifically with reference to Examples, Formulation Examples (Examples of cosmetics and external medicines) and Test Examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all parts are by weight.
[0039]
Embodiment 1 FIG. Preparation of emulsifier (1)
1 Kg of barley is immersed in 70% ethanol overnight to sterilize it, and then, after collecting the barley by filtration, adding 2 L of boil-sterilized water and washing the same three times, the ethanol is sufficiently removed. Removed.
After adding 3 L of boil-sterilized 0.1% yeast extract solution to sterilized barley, a lactic acid bacteria (S. faecalis) culture solution pre-cultured (37 ° C. for 3 days) in GYP medium was added thereto. And the concentration of lactic acid bacteria is 10⁸An amount of 1 / 0.1% yeast extract was added, and the cells were cultured at 37 ° C. for 3 days under a nitrogen atmosphere.
After the cultivation, the lactic acid bacteria fermented product of the oats was fractionated by coarse filtration through a wire mesh, washed with running water, and the attached culture solution was sufficiently washed away. Was reduced to about 15% or less. Next, the dried product was pulverized with a mixer and then sieved (90 μm, 166 mesh) to obtain a fermented product of lactic acid bacteria of barley as a fine powder (yield 920 g, moisture content 12.5%).
[0040]
Embodiment 2. FIG. Preparation of emulsifier (2)
S. lactic acid bacteria faecalis in place of L. The same operation as in Example 1 was performed except that plantalum was used, and a fermented product of lactic acid bacteria of pigeon barley was obtained as a fine powder (yield 910 g, moisture content 12.4%).
[0041]
Embodiment 3 FIG. Preparation of emulsifier (3)
The same operation as in Example 1 was carried out except that barley was used instead of pigeon barley to obtain a fermented product of barley lactic acid bacteria as a fine powder (yield 580 g, moisture content 12.0%).
[0042]
Embodiment 4. FIG. Preparation of emulsifier (4)
The same operation as in Example 1 was carried out except that millet was used in place of pigeon barley to obtain a fermented product of millet lactic acid bacteria as a fine powder (yield 880 g, moisture content 12.8%).
[0043]
Embodiment 5 FIG. Preparation of emulsifier (5)
The same operation as in Example 1 was carried out except that millet was used in place of the pigeon wheat, to obtain a fermented product of millet lactic acid bacteria as a fine powder (yield: 890 g, moisture content: 12.4%).
[0044]
Embodiment 6 FIG. Preparation of emulsifier (6)
The same operation as in Example 1 was carried out except that barley was used instead of pigeon barley to obtain a fermented product of lactic acid bacteria of barley as a fine powder (yield 900 g, moisture content 12.0%).
[0045]
Embodiment 7 FIG. Preparation of emulsifier (7)
The same operation as in Example 1 was carried out except that soybean was used instead of pigeon barley, to obtain a fermented product of lactic acid bacteria of soybean as a fine powder (yield 920 g, moisture content 13.2%).
[0046]
Embodiment 8 FIG. Preparation of emulsifier (8)
The same operation as in Example 1 was carried out except that red bean was used instead of pigeon barley to obtain a fermented product of lactic acid bacteria of red bean as a fine powder (yield 780 g, moisture content 12.5%).
[0047]
Embodiment 9 FIG. Preparation of emulsifier (9)
The soybeans were washed with water, immersed in three times the amount of water overnight, and then drained. This was kept in a wet state at 30 ° C. for 2 days to germinate, and the germinated soybean was dried at 60 ° C. overnight.
1 Kg of the dried germinated soybeans was immersed in 70% ethanol overnight to sterilize, then the germinated soybeans were collected by filtration, and 2 L of boil-sterilized water was added thereto and washed three times. It was sufficiently removed.
To the sterilized germinated soybeans, 3 L of a boil-sterilized 0.1% yeast extract solution was added, and then lactic acid bacteria (S. faecalis) culture pre-cultured (37 ° C., 3 days) in a GYP medium was added thereto. When the concentration of lactic acid bacteria is 10⁸An amount of 1 / 0.1% yeast extract was added, and the cells were cultured at 37 ° C. for 3 days under a nitrogen atmosphere.
After completion of the cultivation, the lactic acid bacteria fermented germinated soybeans were fractionated by coarse filtration through a wire mesh, washed with running water, and the attached culture solution was sufficiently washed away. Was reduced to about 15% or less. Next, the dried product was pulverized with a mixer and then sieved (90 μm, 166 mesh) to obtain a fermented product of germinated soybean lactic acid bacteria as a fine powder (yield: 890 g, moisture content: 13.5%).
[0048]
Embodiment 10 FIG. Preparation of emulsifier (10)
The same operation as in Example 9 was carried out except that barley was used instead of soybean to obtain a fermented product of germinated barley lactic acid bacteria as a fine powder (yield 880 g, moisture content 12.8%).
[0049]
Embodiment 11 FIG. Preparation of emulsifier composition (1)
200 g of maltitol liquid (70% maltitol aqueous solution) was added to 800 g of fine powder of fermented product of pigeon barley lactic acid bacteria obtained in the same manner as in Example 1, and the mixture was kneaded and mixed with a kneading machine to obtain uniform mixing. A product was obtained (water content: 14.6%).
[0050]
Embodiment 12 FIG. Preparation of emulsifier composition (2)
The procedure of Example 11 was repeated, except that 200 g of glycerin was used instead of 200 g of the maltitol solution, to obtain a uniform mixture (water content: 12.4%).
[0051]
Embodiment 13 FIG. Preparation of emulsifier composition (3)
200 g of maltitol solution and 15 g of arginine were added to 785 g of the fine powder of the fermented product of pigeon barley lactic acid bacteria obtained in the same manner as in Example 1 and kneaded and kneaded with a kneading machine to obtain a uniform mixture. (Water content 15.1%).
[0052]
Formulation Example 1. cream
[A component] part
Liquid paraffin 5.0
Olive oil ¥ 4.0
Paraffin $ 5.0
Cetanol 2.0
Butyl paraben 0.1
[B component]
Emulsifier of Example 1 5.0
1,3-butylene glycol 10.0
Methyl paraben 0.1
Purified water = amount of 100 parts
[C component]
Fragrance 0.3
The components A and B were each heated to 80 ° C. or higher, and then the components A and B were added and stirred, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes.
After cooling to 50 ° C., the component C was added and mixed by stirring, and further cooled to 30 ° C. or lower to obtain a homogeneous cream.
[0053]
Formulation example 2. Latex
[A component] part
Liquid paraffin 5.0
Olive oil ¥ 4.0
Squalane $ 5.0
Butyl paraben 0.1
[B component]
Emulsifier of Example 1 5.0
1,3-butylene glycol 10.0
Methyl paraben 0.1
Purified water = amount of 100 parts
[C component]
Fragrance 0.3
The components A and B were each heated to 80 ° C. or higher, and then the components A and B were added and stirred, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes.
After cooling to 50 ° C., the C component was added and mixed with stirring, and further cooled to 30 ° C. or lower to obtain a homogeneous emulsion.
[0054]
Formulation example 3. Lotion
[A component] part
Olive oil 1.0
Butyl paraben 0.1
[B component]
Emulsifier of Example 1 1.0
Ethanol $ 5.0
Glycerin 5.0
1,3-butylene glycol @ 5.0
Methyl paraben 0.1
Purified water = amount of 100 parts
[C component]
Fragrance 0.3
The components A and B were each heated to 80 ° C. or higher, and then the components A and B were added and stirred, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes.
After cooling to 50 ° C., the C component was added and mixed by stirring, and further cooled to 30 ° C. or lower to obtain a milky lotion.
[0055]
Formulation Example 4. essence
[A component] part
Olive oil 1.0
Butyl paraben 0.1
[B component]
Emulsifier of Example 1 2.0
Ethanol $ 5.0
Hyaluronic acid 0.3
1,3-butylene glycol @ 5.0
Methyl paraben 0.1
Purified water = amount of 100 parts
[C component]
Fragrance 0.3
The components A and B were each heated to 80 ° C. or higher, and then the components A and B were added and stirred, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes.
After cooling to 50 ° C., the component C was added and mixed by stirring, and further cooled to 30 ° C. or lower to obtain a milky white essence.
[0056]
Formulation Example 5. Latex
A homogeneous emulsion was obtained in the same manner as in Formulation Example 2 except that 5.0 parts of the emulsifier of Example 1 was used in place of 5.0 parts of the emulsifier in Formulation Example 2.
[0057]
Formulation Example 6. Latex
A homogeneous emulsion was obtained in the same manner as in Formulation Example 2, except that 5.0 parts of the emulsifier of Example 1 was used instead of 5.0 parts of the emulsifier of Example 1 in the B component of Formulation Example 2.
[0058]
Formulation Example 7. Latex
A homogeneous emulsion was obtained in the same manner as in Formulation Example 2, except that 5.0 parts of the emulsifier of Example 1 was used instead of 5.0 parts of the emulsifier of Example 1 in the B component of Formulation Example 2.
[0059]
Formulation Example 8. Latex
A homogeneous emulsion was obtained in the same manner as in Formulation Example 2, except that 5.0 parts of the emulsifier of Example 1 was used instead of 5.0 parts of the emulsifier of Example 1 in the B component of Formulation Example 2.
[0060]
Formulation Example 9. Latex
A homogeneous emulsion was obtained in the same manner as in Formulation Example 2, except that 5.0 parts of the emulsifier of Example 1 was used instead of 5.0 parts of the emulsifier of Formulation 2 in the B component of Formulation Example 2.
[0061]
Formulation Example 10. Latex
A homogeneous emulsion was obtained in the same manner as in Formulation Example 2, except that 5.0 parts of the emulsifier of Example 1 was replaced with 5.0 parts of the emulsifier of Formulation Example 2.
[0062]
Formulation Example 11. Latex
A homogeneous emulsion was obtained in the same manner as in Formulation Example 2 except that 5.0 parts of the emulsifier of Example 1 was replaced with 5.0 parts of the emulsifier of Example 8 in the B component of Formulation Example 2.
[0063]
Formulation Example 12. Latex
A homogeneous emulsion was obtained in the same manner as in Formulation Example 2, except that 5.0 parts of the emulsifier of Example 1 was used instead of 5.0 parts of the emulsifier of Example 1 in the B component of Formulation Example 2.
[0064]
Formulation Example 13. Latex
A homogeneous emulsion was obtained in the same manner as in Formulation Example 2 except that 5.0 parts of the emulsifier of Example 1 was replaced with 5.0 parts of the emulsifier of Example 10 in the B component of Formulation Example 2.
[0065]
Formulation Example 14. Latex
A homogeneous emulsion was obtained in the same manner as in Formulation Example 2, except that 6.0 parts of the emulsifier composition of Example 11 was used in place of 5.0 parts of the emulsifier of Example 1 in the B component of Formulation Example 2.
[0066]
Formulation Example 15. Latex
A homogeneous emulsion was obtained in the same manner as in Formulation Example 2, except that 6.0 parts of the emulsifier composition of Example 12 was used instead of 5.0 parts of the emulsifier of Example 1 in the B component of Formulation Example 2.
[0067]
Formulation example 16. Latex
A homogeneous emulsion was obtained in the same manner as in Formulation Example 2, except that 6.0 parts of the emulsifier composition of Example 13 was used instead of 5.0 parts of the emulsifier of Example 1 in the B component of Formulation Example 2.
[0068]
Formulation example 17. Latex
A homogeneous emulsion was prepared in the same manner as in Formulation Example 2 except that the emulsifier of Formulation Example 2 was replaced with 5.0 parts of the emulsifier of Example 1 and 2.0 parts of the emulsifier of Example 1 and 0.5 part of xanthan gum were used. Obtained.
[0069]
Formulation Example 18. Latex
A homogeneous emulsion was prepared in the same manner as in Formulation Example 2 except that 2.0 parts of the emulsifier of Example 1 and 0.5 part of hyaluronic acid were used in place of 5.0 parts of the emulsifier of Example 1 in the B component of Formulation Example 2. Got.
[0070]
Formulation Example 19. Hair Treatment
[A component] part
Stearyl trimethyl ammonium chloride 5.0
Glyceryl monostearate 1.0
Cetanol 3.0
Octyldodecanol 2.0
[B component]
Emulsifier of Example 1 5.0
1,3-butylene glycol @ 5.0
Hydrolyzed collagen powder 0.5
Methyl paraben 0.1
Purified water = amount of 100 parts
[C component]
Fragrance 0.3
The components A and B were each heated to 80 ° C. or higher, and then the components A and B were added and stirred, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes.
After cooling to 50 ° C., the component C was added, mixed with stirring, and further cooled to 30 ° C. or lower.
[0071]
Formulation example 20. Hair cream
[A component] part
Squalane $ 15.0
Vaseline $ 15.0
Beeswax 2.0
Methyl paraben appropriate dosage
[B component]
Glycerin @ 3.0
1,3-butylene glycol @ 3.0
Xanthan gum 0.1
Emulsifier of Example 2 5.0
Polyoxyethylene hydrogenated castor oil 3.0
Purified water = amount of 100 parts
[C component]
Appropriate amount of fragrance
After the components A and B were each heated and dissolved at 80 ° C., the two components were combined and homogenized with a Hiscotron (5000 rpm) for 2 minutes. This was cooled with stirring, and the C component was added at 50 ° C., and further cooled to 30 ° C.
[0072]
Formulation Example 21. Hair dye / Dye base
[A component] part
Oxidation dye 3.5
Oleic acid 20.0
Stearic acid diethanolamide 3.0
Polyoxyethylene (50) oleyl ether 1.0
[B component]
Emulsifier of Example 3 3.0
1,3-butylene glycol 10.0
Isopropanol 10.0
Ammonia water (28%) 10.0
Sodium sulfite 0.5
Purified water = amount of 100 parts
The components A and B were each heated to 80 ° C. or higher, and then the components A and B were added and stirred, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes.
This was cooled to 30 ° C. or lower while stirring.
[0073]
Formulation Example 22. Liquid Foundation
[A component] part
Stearic acid 2.5
Cetanol 0.5
Glyceryl monostearate 2.0
Lanolin 2.0
Squalane $ 3.0
Isopropyl myristate @ 8.0
Propyl paraben 0.1
[B component]
Emulsifier of Example 1 3.0
Xanthan gum 0.3
1,3-butylene glycol @ 5.0
Triethanolamine 1.0
Methyl paraben 0.1
Purified water = amount of 100 parts
[C component]
Titanium oxide 8.0
Talc $ 4.0
Color pigment appropriate amount
[D component]
Fragrance 0.3
The C component was mixed and pulverized with a pulverizer. The B component was mixed, and the pulverized C component was added thereto, and the mixture was uniformly dispersed in a colloid mill. After heating the A component and the uniformly dispersed B and C components to 80 ° C., respectively, the A component was added to the B and C components while stirring, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes. After cooling to 50 ° C., the D component was added and mixed with stirring, and further cooled to 30 ° C. or lower with further stirring.
[0074]
Formulation Example 23. Cream foundation
[A component] part
Stearic acid $ 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Liquid paraffin 5.0
Squalane $ 3.0
Isopropyl myristate @ 8.0
Propyl paraben 0.1
[B component]
Emulsifier of Example 5５3.0
Sorbitol 3.0
1,3-butylene glycol @ 5.0
Triethanolamine 1.5
Methyl paraben 0.1
Purified water = amount of 100 parts
[C component]
Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Color pigment appropriate amount
[D component]
Fragrance 0.3
The C component was mixed and pulverized with a pulverizer. The B component was mixed, and the pulverized C component was added thereto, and the mixture was uniformly dispersed in a colloid mill. After heating the A component and the uniformly dispersed B and C components to 80 ° C., respectively, the A component was added to the B and C components while stirring, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes. After cooling to 50 ° C., the D component was added and mixed with stirring, and further cooled to 30 ° C. or lower with further stirring.
[0075]
Formulation Example 24. Cream rinse
[A component] part
Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyl dimethyl ammonium chloride 塩 化 1.5
Stearyl trimethyl ammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol @ 3.2
Stearyl alcohol 1.0
Methyl paraben 0.1
[B component]
Emulsifier of Example 1 # 15.0
1,3-butylene glycol @ 5.0
Purified water = amount of 100 parts
The components A and B were each heated to 80 ° C. and uniformly dissolved, and then the component B was added to the component A, and the mixture was stirred and cooled to room temperature.
[0076]
Formulation example 25. Cream shampoo
[A component] part
Sodium N-coconut fatty acid methyltaurine 10.0
Sodium polyoxyethylene (3) alkyl ether sulfate 20.0
Betaine lauryl dimethylaminoacetate 10.0
Coconut oil fatty acid diethanolamide 4.0
Methyl paraben 0.1
[B component]
Citric acid 0.1
Emulsifier of Example 6 # 15.0
1,3-butylene glycol 2.0
Purified water = amount of 100 parts
The components A and B were each heated to 80 ° C. and uniformly dissolved, and then the component B was added to the component A, and the mixture was stirred and cooled to room temperature.
[0077]
Formulation Example 26. Body shampoo
[A component] part
N-lauroylmethylalanine sodium 25.0
Coconut oil fatty acid potassium solution (40%) @ 26.0
Coconut oil fatty acid diethanolamide 3.0
Methyl paraben 0.1
[B component]
Emulsifier of Example 1 10.0
1,3-butylene glycol 2.0
Purified water = amount of 100 parts
The components A and B were each heated to 80 ° C. and uniformly dissolved, and then the component B was added to the component A, and the mixture was stirred and cooled to room temperature.
[0078]
Formulation example 27. Toothpaste
[A component] part
Dibasic calcium phosphate dihydrate ¥ 60.0
Silicic anhydride 2.0
Appropriate amount of fragrance
[B component]
Glycerin 10.0
1,3-butylene glycol @ 5.0
Emulsifier of Example 8 # 5.0
Sodium lauryl sulfate 2.0
Carrageenan 0.3
Methyl paraben appropriate dosage
Saccharin sodium 0.1
Purified water = amount of 100 parts
The component B was mixed, heated and dissolved at 80 ° C, and then cooled to 30 ° C. After the component A was added thereto and kneaded sufficiently, the mixture was defoamed under reduced pressure.
[0079]
Formulation Example 28. Latex
[A component] part
Liquid paraffin 5.0
Olive oil ¥ 4.0
Squalane $ 5.0
Butyl paraben 0.1
[B component]
Emulsifier of Example 1 5.0
1,3-butylene glycol 10.0
Kojic acid 2.0
Methyl paraben 0.1
Purified water = amount of 100 parts
[C component]
Fragrance 0.3
The components A and B were each heated to 80 ° C. or higher, and then the components A and B were added and stirred, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes.
After cooling to 50 ° C., the C component was added and mixed with stirring, and further cooled to 30 ° C. or lower to obtain a homogeneous emulsion.
[0080]
Formulation Example 29. Latex
In the B component of Formulation Example 28, an emulsion was prepared in the same manner as in Formulation Example 28 except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.2 part of potassium hydroxide were used instead of 2.0 parts of kojic acid. Obtained.
[0081]
Formulation example 30. Latex
An emulsion was obtained in the same manner as in Formulation Example 28 except that 3.0 parts of magnesium L-ascorbic acid-2-phosphate was used in place of 2.0 parts of kojic acid in Component B of Formulation Example 28.
[0082]
Formulation Example 31. Latex
An emulsion was obtained in the same manner as in Preparation Example 28 except that 2.0 parts of arbutin was used instead of 2.0 parts of kojic acid in the B component of Preparation Example 28.
[0083]
Formulation example 32. Latex
[A component] part
Liquid paraffin 5.0
Olive oil ¥ 4.0
Squalane $ 5.0
Diisopropylamine dichloroacetate 0.5
Butyl paraben 0.1
[B component]
Emulsifier of Example 3 3.0
1,3-butylene glycol 10.0
Purified water = amount of 100 parts
[C component]
Fragrance 0.3
The components A and B were each heated to 80 ° C. or higher, and then the components A and B were added and stirred, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes.
After cooling to 50 ° C., the component C was added and mixed with stirring, and further cooled to 30 ° C. or lower to obtain an emulsion.
[0084]
Formulation Example 33. Latex
[A component] part
Liquid paraffin 5.0
Olive oil ¥ 4.0
Squalane $ 5.0
γ-amino-β-hydroxybutyric acid 0.5
Butyl paraben 0.1
[B component]
Emulsifier of Example 2 3.0
1,3-butylene glycol 10.0
Purified water = amount of 100 parts
[C component]
Fragrance 0.3
The components A and B were each heated to 80 ° C. or higher, and then the components A and B were added and stirred, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes.
After cooling to 50 ° C., the C component was added and mixed with stirring, and further cooled to 30 ° C. or lower to obtain a homogeneous emulsion.
[0085]
Formulation Example 34. Bath salt
[A component] part
Ethanol $ 5.0
Methyl paraben 0.2
Yellow No. 4 $ 0.1
Fragrance 1.5
[B component]
1,3-butylene glycol @ 5.0
Emulsifier of Example 1 10.0
Purified water = amount of 100 parts
[C component]
Disodium hydrogen phosphate qs
After the component B was heated to 85 ° C. to dissolve it and cooled to room temperature, a solution prepared by mixing and dissolving the component A was added thereto, and then the component C was added to adjust the pH to 7.
[0086]
Formulation Example 35. Antiphlogistic analgesic
[A component] part
Liquid paraffin 5.0
Olive oil ¥ 4.0
Paraffin $ 5.0
Cetanol 2.0
Indomethacin 1.0
Butyl paraben 0.1
[B component]
Emulsifier of Example 1 5.0
1,3-butylene glycol 10.0
Methyl paraben 0.1
Purified water = amount of 100 parts
[C component]
Fragrance 0.3
The components A and B were each heated to 80 ° C. or higher, and then the components A and B were added and stirred, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes.
After cooling to 50 ° C., the component C was added and mixed with stirring, and further cooled to 30 ° C. or lower to obtain an anti-inflammatory analgesic (cream).
[0087]
Formulation Example 36. Anti-inflammatory
[A component] part
Liquid paraffin 5.0
Olive oil ¥ 4.0
Paraffin $ 5.0
Cetanol 2.0
Dexamethasone 0.2
Butyl paraben 0.1
[B component]
Emulsifier of Example 1 5.0
1,3-butylene glycol 10.0
Methyl paraben 0.1
Purified water = amount of 100 parts
[C component]
Fragrance 0.3
The components A and B were each heated to 80 ° C. or higher, and then the components A and B were added and stirred, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes.
After cooling to 50 ° C., the component C was added and mixed by stirring, and further cooled to 30 ° C. or lower to obtain an anti-inflammatory agent (cream).
[0088]
Formulation Example 37. Antifungal agent
[A component] part
Liquid paraffin 5.0
Olive oil ¥ 4.0
Squalane $ 5.0
Tolnaftate 1.0
Butyl paraben 0.1
[B component]
Emulsifier of Example 2 3.0
1,3-butylene glycol 10.0
Purified water = amount of 100 parts
[C component]
Fragrance 0.3
The components A and B were each heated to 80 ° C. or higher, and then the components A and B were added and stirred, and further homogenized with a Hiscotron (5000 rpm) for 2 minutes.
After cooling to 50 ° C., the C component was added and mixed by stirring, and further cooled to 30 ° C. or lower to obtain an antifungal agent (emulsion).
[0089]
Test Example 1 Emulsifying action (1)
The emulsifier of the lactic acid bacteria fermentation product of the cereals of the present invention and the fine powder obtained by crushing the cereals with a mixer and sieving them (90 μm, 166 mesh) for comparison were examined for emulsifying action.
[sample]
(1) Sample of the present invention: each emulsifier of Examples 1, 3, 4, 5, 6, 7 and 8
(2) Comparative sample: fine powder of pigeon barley, barley, millet, millet, millet, soybean and red bean
[Test method]
Emulsions having the component compositions shown in Table 1 were prepared, and the state immediately after the emulsification and changes over time were observed. In addition, the composition A of Table 1 was used for the emulsifiers of Examples 7 and 8, and the fine powder of soybeans and red beans, and the composition B was used for the other samples.
[0090]
[Table 1]

[0091]
That is, the component 4 was dissolved in the component 3, the components 2 and 5 were added thereto, mixed, and heated to 80 ° C. This was added to the oily component of Component 1 heated at 80 ° C., homogenized under the condition of 5000 rpm × 2 minutes, and then cooled with water with stirring to room temperature. Each of the obtained emulsions was placed in a 50-ml screw bottle, and the emulsified state immediately after preparation and the time-dependent change in the emulsified state when stored at room temperature or 50 ° C. for 3 months were visually observed, and evaluated according to the following criteria. did.
◎: good
○: Slight separation is observed after more than one month
Δ: Slight separation is observed after 2 weeks to 1 month
×: Completely separated within one week
In addition, in the case of the comparative samples of the fine powder of barley, fine millet, and fine powder of soybean, oil floating was observed immediately after preparation of the emulsion, and the emulsified state was poor.
[0092]
[result]
Table 2 shows the results.
[Table 2]

[0093]
As shown in Table 2, the emulsifiers comprising the fermented lactic acid bacteria of cereals of the present invention all have excellent emulsifying action and give stable emulsions for a long period of time. On the other hand, a stable emulsion cannot be obtained depending on the fine powder of cereals not subjected to lactic acid bacteria fermentation.
[0094]
Test Example 2. Emulsifying action (2)
The effect on the emulsion stability when a thickener was used in combination with the fermented lactic acid bacteria was investigated.
[Test method]
Three types of emulsions having the component compositions (units: parts) shown in Table 3 were prepared, and their changes over time were observed.
[0095]
[Table 3]

[0096]
That is, the component 4 was dissolved in the component 3, and one of the components 5 was added thereto and mixed. Separately, the mixture of components 2 and 6 and component 1 were each heated to 80 ° C., and then the mixture of components 2 and 6 was added to component 1 and homogenized with a Hiscotron (5000 rpm) for 2 minutes. A mixture of the above components 3, 4, and 5 was added thereto, and the mixture was further homogenized with Hiscotron (5000 rpm) for 1 minute, and then cooled to room temperature with stirring. Each of the obtained emulsions was placed in a 50 ml screw bottle, and the emulsified state immediately after preparation and the time-dependent change in the emulsified state when stored at room temperature or 50 ° C. for 3 months were visually observed, and the same as in Test Example 1. Was evaluated according to the criteria of
[0097]
[result]
Table 4 shows the results.
[Table 4]

[0098]
From the results in Table 4, it can be seen that when a thickener is used in combination with the fermented lactic acid bacteria, the stability of the obtained emulsion is further improved.
[0099]
Test Example 3 Skin irritation
The skin irritation of the fermented product of lactic acid bacteria of the present invention was compared with that of a component commonly used as an emulsifier for cosmetics.
[sample]
The following components were kneaded with Japanese Pharmacopoeia to obtain a 5% concentration of hydrophilic petrolatum and used as a sample.
(1) Emulsifier of Example 1 (sample of the present invention)
(2) polyoxyethylene (20) sorbitan monooleate (comparative sample a)
(3) Lipophilic glyceryl monostearate (Comparative sample b)
[0100]
[Test method]
Five adult males aged 20 to 50 years were taken as subjects, and the inside of each upper arm was wiped with ethanol to remove sebum, and 0.2 g of each sample was applied to the aluminum plate of the fin chamber. Affixed. Twenty-four hours later, the fin chamber was removed, and the degree of skin irritation was determined by the following method and criteria.
[0101]
[Judgment]
At 1 hour, 24 hours and 48 hours after the patch removal, the erythema and edema at the application site were visually determined based on the following “Skin irritation evaluation criteria by Draize method”, and the average value of 5 subjects was determined. I asked.
(Erythema)
Score Skin condition
0: No erythema
1: Very mild erythema
2: Evident erythema
3: Medium to strong erythema
4 ： Crimson erythema and light crust formation
(edema)
Score Skin condition
0: No edema
1: Very mild edema
2: Empty edema (clearly distinguishable from surroundings)
3: Medium edema (1mm or more swelling)
4: Strong edema (spread to surrounding area)
[0102]
[result]
Table 5 shows the results.
[Table 5]

[0103]
The activators a and b used as comparative samples in this test are widely used for emulsification of cosmetics because of their relatively high safety. Emulsifiers composed of fermented lactic acid bacteria have less skin irritation than those emulsifiers and are extremely excellent in safety.
[0104]
Test Example 4. Monitor test
For each of the following samples, the usability and safety (skin irritation) were evaluated by a monitor test.
[sample]
(1) Emulsion of Formulation Example 2 (Emulsion of the present invention)
(2) In Formulation Example 2, it was obtained in the same manner as in Formulation Example 2 except that 2.0 parts of polyoxyethylene (20) sorbitan monooleate was used instead of 5.0 parts of the emulsifier of Example 1. Latex (comparative latex)
[0105]
[Test method]
The subjects were 20 randomly selected females aged 18 to 50 years of age. Each emulsion was applied twice a day (morning and evening) to the left upper arm for 5 days. The following items were evaluated.
(Usability)
I. Feeling picked up
B. Elongation during application
C. Smoothness during application
D. Permeability (feeling of penetration)
E. Feel after application
(Skin irritation)
F. Irritation during application
G. Irritation after application
The evaluation was based on a five-point scale (A: good, B: somewhat good, C: normal, D: slightly poor, E: bad) for the feeling of use, and a three-point scale for skin irritation (A: no irritation, B: discomfort, C: irritation).
[0106]
[result]
The results are shown in Tables 6 and 7.
[Table 6]

[0107]
[Table 7]

[0108]
As shown in Tables 6 and 7, the emulsion prepared using the emulsifier comprising the fermented product of lactic acid bacteria of the present invention was highly evaluated in terms of feeling of use, and no skin irritation was observed.
On the other hand, the comparative emulsion obtained using the synthetic surfactant was not only inferior in the feeling of use but also showed some skin irritation.
【The invention's effect】
The emulsifier of the present invention comprising fermented lactic acid bacteria of cereals has excellent emulsifying power, is excellent in safety to humans such as low toxicity and extremely little skin irritation, and is widely used in cosmetics, external medicines, foods and the like. Useful for preparing emulsions in the field. Above all, when used as an emulsifier for cosmetics and topical medicines, the resulting cosmetics and topical medicines have a feeling and use on the skin because of the high moisturizing and water-retaining ability of the fermented lactic acid bacterium and the adhesiveness to the skin. It will be a good feeling.
Therefore, according to the present invention, there are also provided cosmetics and external medicines comprising the above-mentioned fermented product of lactic acid bacteria and having good emulsification stability and biosafety, as well as good feeling upon use.

Claims (6)

An emulsifier comprising a fermented product of lactic acid bacteria obtained by fermenting one or more kinds of cereals with lactic acid bacteria. The emulsifier according to claim 1, wherein one or more selected from wheat, beans, and millet are used as the millet. The emulsifier according to claim 2, wherein the barley is selected from barley, wheat, and barley. The emulsifier according to claim 2, wherein the beans are selected from soybeans and red beans. The emulsifier according to claim 2, wherein the millet is selected from millet, millet, millet, and corn. A cosmetic or topical medicine comprising a fermented lactic acid bacterium obtained by fermenting one or more cereals with lactic acid bacteria.