KR20220147802A - Antioxidant for cosmetics containing coffee tree leaves extract as an effective ingredient - Google Patents

Abstract

The present invention relates to an antioxidant composition for cosmetics containing a coffee tree leaf extract as an active ingredient. According to the antioxidant composition for cosmetics containing a coffee tree leaf extract as an active ingredient of present invention, by preparing extracts under various conditions through leaves of coffee trees, which are being cultivated in earnest in Korea, comparing antioxidant activity of the extracts, and analyzing the content distribution of active ingredients, manufacturing conditions of the coffee tree leaf extract can be optimized, and the coffee tree leaf extract can be used as a cosmetic raw material with an excellent antioxidant effect. The antioxidant composition contains 0.1 to 20 wt% of the coffee tree leaf extract as an active ingredient.

Description

Antioxidant for cosmetics containing coffee tree leaves extract as an effective ingredient}

The present invention relates to an antioxidant composition for cosmetics containing a coffee tree leaf extract as an active ingredient, and more particularly, as an extract using a natural product, it has no cytotoxicity and stability against side effects, etc., as well as preparation of a coffee tree leaf extract It relates to an antioxidant composition for cosmetics containing, as an active ingredient, a coffee tree leaf extract having excellent antioxidant activity by optimizing conditions.

Coffee, the raw material and product of beverages consumed most not only in Korea but also around the world, contains carbohydrates, lipids, nitrogen compounds such as amino acids, various vitamins and minerals, alkaloids such as caffeine, and phenols such as chlorogenic acid. It contains various components such as compounds.

On the other hand, the coffee tree is a plant included in the genus Coffea of the family Rubiaceae, Coffea It grows to 4-6 m in the case of arabica coffee tree and 8-12 m in the case of C. canephora coffee tree. To harvest coffee fruits, they are grown at a lower height (Anzueto et al. 2005), and the quality is determined by the influence of environmental factors such as altitude, temperature, precipitation, and soil. Among the genus Coffea, which is estimated to have about 80 to 100 species, Coffea arabica ( Arabica coffee ) and Coffea canephora (Robusta coffee) is classified as the most important species in the global coffee market, and even the same cultivar has different taste and aroma characteristics depending on the region or environment where it is grown.

Most of the coffee consumed in Korea is imported from abroad, and major producers include Ethiopia, Colombia, Mexico, Guatemala, the Philippines, Indonesia, and India. Recently, in Korea, cultivation has started for the purpose of tourism, processing and sales, centered on southern regions such as Jeonju and Jeju, and the amount of cultivation and coffee production are also increasing little by little. Accordingly, it is necessary to study the possibility of using parts other than the part of the fruit including coffee seeds consumed by the existing processing method. In particular, the leaf part that can be used for tea, etc. It is necessary to increase the usability of

However, there is still no study on cosmetic compositions having antioxidant effects using coffee tree leaf extracts. Therefore, in the present invention, by preparing extracts under various conditions through the leaves of coffee trees that are being cultivated in earnest in Korea, comparing antioxidant activity and analyzing the content distribution for active ingredients, the manufacturing conditions of coffee tree leaf extracts are optimized Therefore, the development of natural materials with antioxidant effects is required.

KR 10-1655276 B1 (2016. 09. 01.)

The present invention has been devised to solve the problems of the prior art, and the problem to be solved in the present invention is to compare antioxidant activity by preparing extracts under various conditions from the leaves of coffee trees, which are being cultivated in earnest in Korea. , to optimize the production conditions of the coffee tree leaf extract by analyzing the content distribution for the active ingredient, and to provide a cosmetic composition having an antioxidant effect.

Another purpose is to prepare extracts under various conditions to examine the various uses of coffee tree leaves, compare antioxidant activity and check the content distribution for active ingredients, thereby providing basic data for the development of related products using coffee tree leaves Its purpose is to provide

The antioxidant composition for cosmetics containing the coffee tree leaf extract as an active ingredient according to the present invention for achieving the above object is characterized in that it contains 0.1 to 20% by weight of the coffee tree extract as an active ingredient.

In addition, the coffee tree leaf extract is washed and dried so that the moisture content is 5% or less, and 20% ethanol is mixed with the coffee tree leaves ground to a size of 50-100 mesh in a ratio of 1: 10-20 by weight, 20 It is characterized in that it is prepared by filtration after shaking extraction for 2-3 hours at a reciprocating speed of 150 to 200 times per minute at ℃.

In addition, the coffee tree leaf extract is washed and dried so that the moisture content is 5% or less, and purified water is mixed with the coffee tree leaves ground to a size of 50-100 mesh in a ratio of 1: 10-20 by weight, and at 100 ° C. It is characterized in that it is prepared by filtration after extraction for 60 to 90 minutes.

In addition, the composition has any one formulation selected from flexible lotion, astringent lotion, nourishing lotion, lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder characterized.

According to the antioxidant composition for cosmetics containing the coffee tree leaf extract of the present invention as an active ingredient, extracts under various conditions are prepared from the leaves of coffee trees, which are being cultivated in earnest in Korea, to compare antioxidant activity, By analyzing the content distribution, there is an effect of optimizing the manufacturing conditions of the coffee tree leaf extract and using it as a cosmetic raw material having excellent antioxidant efficacy.

1 is a diagram showing liquid chromatography of a coffee tree leaf extract according to ethanol solvent concentration according to the present invention.
Figure 2 is a view showing the liquid chromatography of the coffee tree leaf extract for each hot water extraction time according to the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The present invention relates to an antioxidant composition for cosmetics containing a coffee tree leaf extract as an active ingredient.

The coffee tree leaf extract according to the present invention is prepared by solvent extraction or hot water extraction of coffee tree leaves through 20% ethanol (alcohol), has no cytotoxicity, stability against side effects, etc. It was confirmed that there is an excellent antioxidant activity through

The coffee tree leaf extract of the present invention can be prepared through ethanol solvent extraction.

More specifically, 20% ethanol is mixed with coffee tree leaves that have been washed and dried to a moisture content of 5% or less and ground to a size of 50-100 mesh in a ratio of 1: 10-20 by weight, and heated at 20 ° C. It is prepared by filtration after shaking extraction for 2 to 3 hours at a reciprocating speed of 150 to 200 times.

Here, using 20% ethanol as an extraction solvent is to prepare a coffee tree leaf extract using 5, 10, 20, 40, 60, and 80% ethanol under the same conditions, respectively, and the radical scavenging ability according to the ethanol solvent concentration conditions And as a result of measuring the active ingredient content, 20% ethanol showed the best effect.

In addition, when the mixing amount of the extraction solvent, the extraction temperature, and the extraction time are out of the range, the extraction efficiency may decrease or a change in components may occur.

The coffee tree leaf extract of the present invention may be prepared through hot water extraction.

More specifically, purified water is mixed with coffee tree leaves that have been washed and dried to a moisture content of 5% or less and ground to a size of 50-100 mesh in a ratio of 1: 10-20 by weight, and 60-90 at 100 ° C. After extraction for minutes, it is prepared by filtration.

Here, to prepare the extract by heating for 60 to 90 minutes through purified water as an extraction solvent, each extract is prepared for each hour at intervals of 0, 10, 20, 30, 60 and 90 minutes under the same conditions, and according to the extraction time conditions As a result of measuring the radical scavenging ability and the content of active ingredients, the best effect was shown in 60 minutes or longer.

In addition, when the mixing amount of the solvent and the extraction temperature are out of the range, the extraction efficiency may decrease or the component may change.

The coffee tree extract may be used as a cosmetic composition having an antioxidant effect.

In addition, it is preferable to include 0.1 to 20% by weight of the coffee tree extract as an active ingredient with respect to the entire composition.

It is preferable that the content of the composition be equal to or greater than the minimum value to achieve the minimum effect, and the content of the composition is preferably equal to or less than the maximum value in consideration of the decrease in feeling of use due to excessive addition and applicability to various formulations.

In addition, in the present invention, in the extraction process of the coffee tree leaf extract, in order to improve the antioxidant ability by increasing the content of total phenolic components of the coffee tree leaf extract, a base selected from NaOH and KOH based on 100 parts by weight of the extraction solvent or 0.1 to 10 parts by weight of an acid selected from HCl and CH ₃ COOH may be further added.

If the added content of the base or acid is less than the above range, the amount of increase in the total phenolic components in the coffee tree leaf extract is insignificant. not.

In addition, in order to increase the content of the total phenolic components of the extract to improve the antioxidant ability, the coffee tree leaf powder was subjected to cellulase, pectinase, hemicellulase, arabinase before the extraction process. (arabinase), beta-glucanase (β-glucanase), xylanase (xylanase), amylase (amylase), treated with at least one hydrolytic enzyme selected from the group consisting of amyloglucosidase (amyloglucosidase) process may include.

In addition, the antioxidant composition for cosmetics containing the coffee tree leaf extract of the present invention as an active ingredient can be prepared in any formulation conventionally prepared in the art, for example, a solution, a suspension, an emulsion, a paste, a gel. , cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, to be prepared in any one formulation selected from flexible lotion, astringent lotion, nourishing lotion, lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder. can

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and additionally chlorofluorohydrocarbon, propane/butane or dimethyl ether It may contain propellants such as

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-Butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar, or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glycerin, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used.

The ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions, in addition to the active ingredient and carrier ingredient, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. may include

The cosmetic composition of the present invention includes a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamin may include any type of vitamin that can be formulated in cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, and vitamins. H, etc., and their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) can also be used in the present invention. included in water-soluble vitamins. Water-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a microbial culture, an enzymatic method or a chemical synthesis method.

The oil-soluble vitamin may include any type of vitamin that can be formulated in cosmetics, but preferably includes vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol), and the like. , their derivatives (ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether) and the like are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a microbial culture, an enzyme or a chemical synthesis method, and the like.

The macromolecular peptide may include any compound that can be formulated in cosmetics, but preferably includes collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial culture, enzymatic methods or chemical synthesis, or can be purified and used from natural products such as dermis of pigs or cattle, silk fibers of silkworms, and the like.

The high-molecular polysaccharide may include any compound that can be formulated in cosmetics, but preferably hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be used after purification from mammals or fish.

The sphingolipid may include any type of sphingolipid as long as it can be formulated in cosmetics, but preferably ceramide, phytosphingosine, sphingoglycolipid, and the like. Sphingo lipids can be purified by conventional methods from mammals, fish, shellfish, yeast or plants, or can be obtained by chemical synthesis.

The seaweed extract may include any compound as long as it can be formulated in cosmetics, but preferably brown algae extract, red algae extract, green algae extract, and the like, and calrageenan, arginic acid, and ares purified from these seaweed extracts Sodium ginate, potassium alginate, etc. are also included in the seaweed extract used in the present invention. The seaweed extract can be obtained by purification from seaweed by a conventional method.

In the cosmetic composition of the present invention, in addition to the above essential ingredients, other ingredients usually blended in cosmetics may be blended as needed. Other ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohols, colorants, fragrances , a blood circulation promoter, a cooling agent, an anti-inflammatory agent, purified water, and the like.

The fats and oils may include ester-based fats and oils, hydrocarbon-based fats and oils, silicone-based fats and oils, fluorine-based fats and oils, animal fats and oils, vegetable oils and the like.

As ester-based oils and fats, tri2-ethylhexaneglyceryl, 2-ethylhexanecetyl, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate , ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diethyl sebacate Isopropyl, isoalkyl neopentanoate, tri (caprylic acid, capric acid) glyceryl, trimethylol propane tri2-ethylhexanoate, trimethylol propane triisostearate, pentaelysitol tetra2-ethylhexanoate, Cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostearyl laurate , Isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isostearic acid isopropyl, 2-ethylhexanoate cetostearyl , 2-ethyl stearyl hexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, di(capryl, capric acid) propylene glycol, propylene glycol dicaprylate, dicaprine Neopentyl glycol acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecyl acid, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, Octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleic acid ester, polyglycerol isostearate ester, citric acid Triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisomalate Stearyl, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, stearate Cholesteryl ester, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stearoylhydr and esters such as isocetyl hydroxystearate, stearyl 12-stealoylhydroxystearate, and isostearyl 12-stealoylhydroxystearate.

The hydrocarbon-based fats and oils may include hydrocarbon-based fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, petrolatum.

Silicone-based fats and oils include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylstealloxysiloxane copolymer, alkyl-modified silicone oil, amino-modified silicone oil, and the like.

The fluorine-based oil may include perfluoropolyether and the like.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, bird flower oil, soybean oil, corn oil, rapeseed oil, sesame oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, Coconut oil, Kukui nut oil, wheat germ oil, rice germ oil, shea butter, cranberry colostrum oil, marker damia nut oil, meadow home oil, egg yolk oil, tallow, horse oil, mink oil, orange raffia oil, jojoba oil, candelabra wax, karr It may contain animal or vegetable oils such as nava wax, liquid lanolin, hydrogenated castor oil, and the like.

The moisturizing agent may include a water-soluble low-molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, a fat-soluble polymer, and the like.

Water-soluble low molecular weight moisturizers include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), poly Propylene glycol (degree of polymerization n = 2 or more), polyglycerin B (degree of polymerization n = 2 or more), lactic acid, lactate, and the like may be included.

The fat-soluble low molecular weight moisturizer may include cholesterol, cholesterol esters, and the like.

Water-soluble polymers include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, dextrin, etc. may include

The fat-soluble polymer may include polyvinylpyrrolidone/eicocene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, high molecular weight silicone, and the like.

The emollient agent may include long-chain acylglutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

Surfactants may include nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, and the like.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE/POP (polyoxyethylene/polyoxypropylene) copolymer, POE/POP alkyl ether, polyether-modified silicone, laurin acid alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of anionic surfactants include fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate, alkyl Amide phosphate, alkyloyl alkyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, alkyl sulfoacetate sodium, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. can do.

Cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride, diethylaminoethyl stearate, dimethylaminopropyl stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amidesulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amidamine type, etc. of amphoteric surfactants and the like.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, oxide Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, calamine and complexes thereof, polyamide, polyester, polypropylene, polystyrene, poly Urethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene/styrene copolymer, silk powder, cellulose, CI pigment yellow, CI pig Organic pigments, such as menthol orange, and complex pigments of these inorganic pigments and organic pigments may be included.

Examples of the organic powder include metal soaps such as calcium stearate; alkyl phosphate metal salts such as sodium cetylate, zinc laurylate and calcium laurylate; acylamino acid polyvalent metal salts such as calcium N-lauroyl-beta-alanine, zinc N-lauroyl-beta-alanine, and calcium N-lauroyl glycine; amidesulfonic acid polyvalent metal salts such as calcium N-lauroyl-taurine and calcium N-palmitoyl-taurine; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hydrogenated beef fatty acid acylarginine, etc. -acyl basic amino acids; N-acyl polypeptides such as N-lauroylglycylglycine; alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like may be included.

UV absorbers include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate, cinnamic acid. Benzyl, para-methoxycinnamic acid-2-ethoxyethyl, para-methoxycinnamic acid octyl, dipara-methoxycinnamic acid mono-2-ethylhexane glyceryl, para-methoxycinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture; Urocanic acid, ethyl urokanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenonedisulfonate, dihydroxybenzo Phenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, and the like.

Disinfectants include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc phyllithione, benzalkonium chloride, Mononitroguarechol sodium, undecyrenic acid, and the like may be included.

Antioxidants may include butylhydroxyanisole, propyl gallic acid, elisorbic acid, and the like.

The pH adjuster may include citric acid, sodium citrate, malic acid, sodium malate, fmalic acid, sodium fmalate, succinic acid, sodium succinate, sodium hydroxide, and the like.

The alcohol may include a higher alcohol such as cetyl alcohol.

Hereinafter, the present invention will be described in more detail through examples. However, it is clear to those skilled in the art that the following examples are only for specifically illustrating the present invention, and do not limit the scope of the present invention. That is, simple modifications or changes of the present invention can be easily carried out by those skilled in the art, and all such modifications or changes can be considered to be included in the scope of the present invention.

Example 1: Preparation of coffee tree leaf extract according to ethanol solvent concentration conditions

The coffee tree leaves used in the present invention were collected from coffee trees of Coffea arabica L. species cultivated in Jeonju.

The coffee tree leaf extract was washed, dried so that the moisture content was 5% or less, and ethanol was mixed with the coffee tree leaves ground to a size of 60 mesh in a ratio of 1:15 to the weight, and at a reciprocating speed of 180 times per minute at 20°C, 2 It is prepared by shaking extraction for a period of time and then filtration.

At this time, coffee tree leaf extracts were prepared using 5, 10, 20, 40, 60 and 80% ethanol, and efficacy evaluation according to the ethanol solvent concentration condition was measured. The results are shown in Experiments 1 and 2.

Example 2: Preparation of coffee tree leaf extract according to hot water extraction time conditions

The coffee tree leaves used in the present invention were collected from coffee trees of Coffea arabica L. species cultivated in Jeonju.

The coffee tree leaf extract is prepared by washing, drying so that the moisture content is 5% or less, mixing purified water with the coffee tree leaves ground to a size of 60 mesh in a weight ratio of 1:15, extracting at 100°C, and filtering.

At this time, extracts were prepared for each hour at intervals of 0, 10, 20, 30, 60 and 90 minutes for extraction time, and efficacy evaluation according to the hot water extraction time condition was measured. The results are shown in Experiments 3 and 4.

Experiment 1: Measurement of antioxidant activity according to ethanol solvent concentration conditions (DPPH and ABTS radical scavenging ability)

In order to confirm the antioxidant activity according to the ethanol solvent concentration condition of Example 1, radical scavenging ability was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Each extract was diluted in methanol to prepare 20, 50 and 100% concentrations. After mixing 150 μL of the DPPH solution dissolved in 100 μM with methanol and 50 μL of the sample solution, the mixture was reacted in the dark for 20 minutes to measure the absorbance at 517 nm. The DPPH radical scavenging ability of each extract was calculated based on the absorbance of the control group using methanol instead of the extract, and then compared.

To confirm the antioxidant activity, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability was measured. Each extract was diluted in methanol to prepare 20, 50 and 100% concentrations. After mixing 7.4mM ABTS and 2.6mM potassium persulfate, the mixture was left at room temperature and in a dark place for 4 hours to form radicals, and it was diluted with distilled water so that the absorbance at 735nm was 0.700±0.05 just before the experiment. 50 μl of the sample solution and 150 μl of ABTS solution were mixed and left in the dark for 30 minutes, and then absorbance was measured at 735 nm. The ABTS radical scavenging ability of each extract was calculated based on the absorbance of the control group using methanol instead of the extract, and then compared. The results are shown in Table 1.

Radical scavenging ability of coffee tree leaf extract according to ethanol solvent concentration condition sample concentration
(%) Extraction conditions (ethanol concentration) 5% 10% 20% 60% 80%
DPPH radical scavenging ability 100 42.2 60.2 74.8 61.4 42.6 50 23.1 33.8 40.4 33.0 22.3 12.6 20 7.2 11.8 14.6 10.2 7.4 4.9
ABTS radical scavenging ability 100 28.8 44.0 52.9 42.8 30.7 19.5 50 15.3 19.6 24.7 19.0 17.2 11.9 20 6.0 8.2 10.4 5.9 3.8

* The unit is %, and each value is an average of three measurements

As shown in Table 1, in the DPPH radical scavenging ability of the coffee tree leaf extract according to the ethanol solvent concentration condition, the 20% ethanol extract using the stock solution (100%) exhibited 74.8% of the DPPH radical scavenging ability. It showed a higher radical scavenging ability compared to that. The same result was shown at the 50% and 20% sample concentrations, and the scavenging ability trend according to the ethanol concentration showed that the scavenging ability also increased as the ethanol concentration increased up to the 20-40% level, but the extract with an ethanol concentration higher than 40% Rather, it showed a phenomenon that the elimination ability was lowered. In particular, the 80% ethanol extract showed significantly lower scavenging ability than the 5% ethanol extract with the lowest concentration. In the ABTS radical scavenging ability measurement, like the DPPH radical scavenging ability, the 20% ethanol extract showed the best radical scavenging ability, and it was confirmed that the scavenging ability gradually decreased in the ethanol extract of 40% or more.

Experiment 2: Comparison of active ingredient content according to ethanol solvent concentration conditions

In order to compare the active ingredient content according to the ethanol solvent concentration conditions of Example 1, a liquid chromatography analysis with an ultraviolet detector was performed. Liquid chromatography was performed using the LC-30A (Shimadzu, Kyoto, Japan) system, and analysis was performed using C18 (2.1mm × 150mm, 2.6μm, Phenomenex, Torrance, CA, USA) as the analytical column as the basic equipment. did. The coffee tree leaf extract for each condition was filtered through a 0.45 μm syringe filter and used for measurement, the sample injection amount was 5 μL, and the oven temperature was maintained at 40°C. Using water (a) and acetonitrile (b) containing 0.1% formic acid as mobile phases, starting from 5% b and sequentially changing to 100% b, the analysis was conducted for 45 minutes by lowering it to 0% b again, The flow rate was maintained at 0.3 mL/min. The wavelength of the UV detector was set to 275 nm and 320 nm to facilitate the detection of coffee active ingredients such as caffeine and chlorogenic acid. After calculating the peak area of each active ingredient in the chromatogram obtained as a result of the analysis, the relative content was calculated by setting the value of the 80% ethanol extract to 100. The results are shown in Figure 1 and Table 2.

Relative content of active ingredients in coffee tree leaf extract according to ethanol solvent concentration conditions
Ingredient name Extraction conditions (ethanol concentration) 5% 10% 20% 60% 80% Caffeine (C) 69.27 72.90 79.67 90.02 96.66 100.00 neo-Chlorogenic acid (A) 162.44 207.25 208.44 172.50 143.42 100.00 Chlorogenic acid (B) 89.21 113.40 127.14 125.14 116.28 100.00

* The ratio calculated by setting the value of 80% ethanol extract to 100 for each component.

1, as a result of liquid chromatography analysis to compare the content of active ingredients in the coffee tree leaf extract according to the ethanol solvent concentration condition, caffeine (C ) and polyphenol components, chlorogenic acid (B) and neo-chlorogenic acid (A) were identified as major components.

In addition, as shown in Table 2, it was confirmed that caffeine(C) had a higher content as the ethanol concentration was higher in the relative content results obtained by calculating the content of the 80% ethanol extract having the highest ethanol concentration as the reference (100). However, chlorogenic acid (B) and neo-chlorogenic acid (A), which are polyphenol components, showed different aspects, respectively, and chlorogenic acid (B) showed a high level of 125.14 to 127.14 at 20-40% ethanol concentration, Neo-chlorogenic acid (A) showed a high content level of 207.25 to 208.44 at a concentration of 10 to 20% ethanol. As a result, when ethanol was used as the extraction solvent, it was confirmed that 20% ethanol concentration was the most favorable condition for extracting the active ingredient based on the polyphenol component excluding the caffeine content.

Chlorogenic acid (B) and neo-chlorogenic acid (A) have the same molecular weight and chemical formula, except that the caffeoyl- structure is bonded to the 3rd or 5th position in the molecular structure. It is one of the representative polyphenols present in coffee. However, although it is known through several reports that the physiological activity is somewhat different, it is basically reported to have a similar activity tendency. Antioxidant activity (Xi et al. 2017) and anti-inflammatory activity (Kim et al. 2017) have been reported as representative physiological activities of chlorogenic acid. Neo-chlorogenic acid also has antioxidant activity (Liu et al. 2015) and anti-inflammatory activity. (Kim et al. 2015) and the like are known to be excellent.

Experiment 3: Measurement of antioxidant activity according to hot water extraction time conditions (DPPH and ABTS radical scavenging ability)

In order to confirm the antioxidant activity according to the hot water extraction time condition of Example 2, radical scavenging ability was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Each extract was diluted in methanol to prepare 20, 50, and 100% concentrations. After mixing 150 μL of the DPPH solution dissolved in 100 μM with methanol and 50 μL of the sample solution, the mixture was reacted in the dark for 20 minutes to measure the absorbance at 517 nm. The DPPH radical scavenging ability of each extract was calculated based on the absorbance of the control group using methanol instead of the extract, and then compared.

To confirm the antioxidant activity, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability was measured. Each extract was diluted in methanol to prepare 20, 50, and 100% concentrations. After mixing 7.4mM ABTS and 2.6mM potassium persulfate, the mixture was left at room temperature and in a dark place for 4 hours to form radicals, and it was diluted with distilled water so that the absorbance at 735nm was 0.700±0.05 just before the experiment. 50 μl of the sample solution and 150 μl of ABTS solution were mixed and left in the dark for 30 minutes, and then absorbance was measured at 735 nm. The ABTS radical scavenging ability of each extract was calculated based on the absorbance of the control group using methanol instead of the extract, and then compared. The results are shown in Table 3.

Radical scavenging ability of coffee tree leaf extract according to hot water extraction time condition sample concentration
(%) Extraction conditions (ethanol concentration) 0 minutes 10 minutes 20 minutes 30 minutes 60 minutes
DPPH radical scavenging ability 100 10.8 17.3 21.1 30.9 35.7 50 6.3 9.9 13.4 15.0 17.4 20.2 20 1.7 4.3 4.9 6.2 8.0 8.4
ABTS radical scavenging ability 100 9.5 15.1 18.4 22.9 27.0 31.3 50 5.8 9.2 10.0 12.8 13.2 20 2.6 4.2 4.6 6.0 9.4

* The unit is %, and each value is the average of three average measurements.

As shown in Table 3, in the measurement results of the DPPH radical scavenging activity of the hot water extract of coffee tree leaves according to the hot water extraction time conditions, it was confirmed that the scavenging ability increased as the extraction time increased after reaching 100 °C. The same trend was shown. In the measurement of the ABTS radical scavenging ability, like the DPPH radical scavenging ability, the scavenging ability increased as the extraction time increased.

Experiment 4: Comparison of active ingredient content according to hot water extraction time conditions

In order to compare the active ingredient content according to the hot water extraction time conditions of Example 2, a liquid chromatography analysis equipped with an ultraviolet detector was performed. For liquid chromatography, the LC-30A (Shimadzu, Kyoto, Japan) system was used, and analysis was performed using C18 (2.1mm × 150mm, 2.6μm, Phenomenex, Torrance, CA, USA) as an analytical column as the basic equipment. did. The coffee tree leaf extract for each condition was filtered through a 0.45 μm syringe filter and used for measurement, the sample injection amount was 5 μL, and the oven temperature was maintained at 40°C. Using water (a) and acetonitrile (b) containing 0.1% formic acid as mobile phases, starting from 5% b and sequentially changing to 100% b, the analysis was conducted for 45 minutes by lowering it to 0% b again, The flow rate was maintained at 0.3 mL/min. The wavelength of the UV detector was set to 275 nm and 320 nm to facilitate the detection of coffee active ingredients such as caffeine and chlorogenic acid. After calculating the peak area of each active ingredient in the chromatogram obtained as a result of the analysis, the relative content was calculated by setting the value of the 80% ethanol extract to 100. The results are shown in Figure 2 and Table 4.

Relative content of active ingredients in coffee tree leaf extract according to hot water extraction time conditions
Ingredient name Extraction conditions (ethanol concentration) 0 minutes 10 minutes 20 minutes 30 minutes 60 minutes Caffeine (C) 35.43 35.67 34.81 33.66 31.99 31.28 neo-Chlorogenic acid (A) 30.51 53.72 72.91 87.91 122.18 147.73 Chlorogenic acid (B) 32.07 47.18 54.02 57.95 64.44 67.20

* The ratio calculated by setting the value of 80% ethanol extract to 100 for each component.

As shown in Fig. 2, liquid chromatography analysis was performed to compare the content of active ingredients in the hot water extract of coffee tree leaves according to the hot water extraction time condition, and as a result, caffeine ( C) as polyphenol components, chlorogenic acid (B) and neo-chlorogenic acid (A) were identified as major components.

In addition, as shown in Table 4, it was confirmed that the content of Caffeine (C) was generally significantly lower than that of the ethanol extract by concentration, and it was confirmed that the content of Caffeine (C) decreased slightly rather than increased with the heating time. In other words, it was confirmed that caffeine(C) could not be extracted as it is in the amount of coffee tree leaves under the hot water extraction conditions, and it was found that the extraction method was suitable for the condition that the caffeine(C) content should be low.

The contents of chlorogenic acid (B) and neo-chlorogenic acid (A) were 67.20 and 147.73 in the hot water extract heated for 90 minutes, showing the highest levels among the hot water extracts, and it was confirmed that the content also increased relatively as the heating time increased. did. In particular, it can be seen that the relatively higher polarity of neo-chlorogenic acid (A) has a high increase. Considering that there is no significant change in the caffeine content in the case of hot water extraction using water as an extraction solvent, it is judged that the extraction time is 60 minutes or more, which shows that it is a favorable condition for extracting the active ingredient.

Claims (5)

An antioxidant composition for cosmetics containing coffee tree extract as an active ingredient.
An antioxidant composition for cosmetics containing 0.1 to 20% by weight of coffee tree extract as an active ingredient.
According to claim 1,
The coffee tree leaf extract,
After washing and drying the moisture content to 5% or less, 20% ethanol is mixed with coffee tree leaves ground to a size of 50-100 mesh in a ratio of 1:10-20 to weight, and 150-200 round trips per minute at 20°C Antioxidant composition for cosmetics, characterized in that it is prepared by filtration after shaking extraction for 2 to 3 hours at a speed.
According to claim 1,
The coffee tree leaf extract,
After washing, drying the moisture content to 5% or less, and mixing purified water with the coffee tree leaves ground to a size of 50-100 mesh in a weight ratio of 1: 10-20, extracting at 100 ° C for 60-90 minutes, and filtering Antioxidant composition for cosmetics, characterized in that produced by
According to claim 1,
The composition is
For cosmetics, characterized in that it has any one formulation selected from flexible lotion, astringent lotion, nourishing lotion, lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder. Antioxidant composition.

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