KR101730624B1 - Composition for skin-whitening comprising extract of Boehmeria nivea (L.) GAUDICH Leaf - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening comprising an extract of ramie leaves as an active ingredient.
According to the present invention, the ramie leaf extract of the present invention effectively inhibits tyrosinase activity, which is an enzyme essential for the melanin biosynthetic process, and inhibits melanin biosynthesis of melanoma cells, thereby exhibiting a skin whitening effect.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for skin whitening comprising an extract of Panax notogensis extract as an active ingredient (Composition for skin-whitening comprising extract of Boehmeria nivea (L.) GAUDICH Leaf}

More particularly, the present invention relates to a cosmetic composition for whitening skin comprising an extract of ramie leaves as an active ingredient.

Melasma is a major factor in skin pigmentation, which is caused by an increase in melanin in the epidermis. Melanin is produced in the organelle called melanosom in the melanocyte present in the epidermal basal layer. That is, mitosis of melanocytes occurs by ultraviolet rays, and melanocytes are then activated. In the activated melanocyte, the synthesis of tyrosinase is promoted and the production of melanin is enhanced, thereby transporting and discharging it out of the epidermis (VJ Hearing and M. Jimenez., Int. J. Biochem, 19 (20) p.1141, 1987., I. Koiso., Fragrance J. 6, p39, 1990).

Naturally occurring melanin is a mixture of alkaline insoluble black melanin (neomelanin) and alkali soluble yellow or red melanin (Y. Tomita., Fragrance J, 6, p20, 1990.). In general, melanin polymers are often referred to as neomelanin. Prota (G. Prota., J. Invest. Dermatol., 75, p122, 1980.) and Pavel (S. Pavel et al., Cancer Detection and Prevention, 6, p311., 1983.), it has been reported that the neuraminins are not only the 5,6-dihydroxyindole that has been contemplated yet, but also the 5,6-dihydroxyindole (5,6-dihydroxyindole-2-carboxylic acid), and various melanin intermediate metabolites. Melanogenesis in melanomas is caused by the oxidation of tyrosine by enzyme tyrosinase to DOPA → dopaquinone → dopachrome → 5,6-dihydroxyindole (5,6-dihydroxyindole ) → indole-5,6-quinone, and is known to produce melanin by polymerization of indole-5,6-quinone. Among them, tyrosine-dopaquinone is known to be involved in enzyme tyrosinase, and thereafter, it is known that autoxidation contributes greatly. It is well known that the pigmentation caused by sunbathing, that is, the blackening phenomenon is a result of the increase of melanin production by the ultraviolet rays in sunlight.

The mechanism of inhibition of melanogenesis from the melanin production process in melanocytes, which has been clarified so far, is as follows. The mechanism of inhibiting melanogenesis by inhibiting the cytotoxicity of melanocytes and the inhibition of melanogenesis in melanocytes, And a mechanism of inhibitory action. Recently, it has been reported that tyrosinase related protein 1 (TRP-1) and tyrosinase-related protein 2 (TRP-2, Dopachrome tautomerase) as well as tyrosinase can inhibit melanin These are also important enzymes involved in the biosynthesis of melanin. Development of substances capable of inhibiting these enzymes can also be a way to prevent skin pigmentation (Marmol V. et al. , FEBS Letter, 327, p. 307, 1993; Winder AJ et al., Pigment Cell Research, 7, p.

The tyrosinase activity inhibitors developed to date include chelating agents for tyrosinase active sites of copper ions, reducing agents such as vitamin C or kojic acid that reduce quinones to phenols, and tyrosinase itself And a bisulfite preparation for modifying the composition. In addition, hydroquinone, retinol or hydrocortisol inhibits tyrosinase activity and is known as a substance exhibiting a whitening effect. Among these, skin whitening substances obtained by chemical synthesis are highly likely to cause adverse effects due to toxicity to the skin.

Therefore, recently, it has been widely known that substances having an absorbing action, which is extracted and separated from natural materials, are usually less irritating to skin and have other pharmacological actions such as astringent action and antibacterial action, Various attempts to manufacture have been studied.

As a result of the above-mentioned attempts, the present inventors have been studying natural products such as rape leaves ( Boehmeria nivea (L.) GAUDICH ) belongs to the nettle family. It is 1 ~ 2m in height. Its stem is round and green, its branches are split, and there are many fine hairs spread with petiole. Leaves are opposite to each other, round egg-shaped, 10 ~ 15cm long, 6 ~ 12cm wide, long end like tail, sawtooth on edge, dark green on front side of leaves, little hair on leaf but back side of leaf is fluffy Feature. Leaf stalk is similar in length to leaf or slightly short and has fine hairs. Flowers bloom in July ~ August, and male and female are hanging on the axilla, shorter than the petiole, and the female flower is in the lower part of the stem, the female flower is in the upper part of the stem, and the flower is 5 ~ 10cm long. The male flower has 4 hairs and 4 stamens. The female flower is wrapped in a tubular horn and consists of 1 pistil. Fruits are oval, oval shaped, several attached together and 1mm long. It is a perennial herbaceous plant. Its origin is in Korea and it is distributed in the southernmost part of the country. It lives in a humid and warm region. The ramie leaves are used to lower and decipher heat, to stop bleeding and to eliminate eosinophils. It also makes the urine come out well, stabilizes the fetus and also shows the anti-inflammatory action. There is a fever with thirst, urine incontinence, hemorrhage, hemorrhage, intestinal hemorrhage, fever, sudden anxiety (gestation anxiety), solitary, swelling, tarnishing, snake bite. Bronchitis, urethritis can also be used. The main components are chlorogenic acid, leuproline, apigenin, and protocatechinic acid. Studies on flax seeds have been reported to inhibit obesity, prevent hair loss, and have antimicrobial efficacy.

Accordingly, the present inventors searched for a substance that is effective for skin whitening and improvement, and found that skin-whitening effective substances were screened out among various natural substances of nature. As a result, , Thereby completing the present invention.

Accordingly, it is a main object of the present invention to provide a skin whitening cosmetic composition containing an extract of ramie leaves having an effect of skin whitening as an active ingredient.

According to one aspect of the present invention, there is provided a skin whitening cosmetic composition comprising an extract of Momordica sativa as an active ingredient.

The inventors of the present invention conducted screening of substances effective for skin whitening among various natural plants in order to search for a method for skin whitening by controlling tyrosinase, an enzyme that produces pigmentation and produce melanin and melanin, which is a factor for determining skin color , And it was confirmed that the component extracted from ramie leaf had excellent skin whitening effect, and the present invention was completed.

In the present invention, the extract is characterized by being a crude extract, a polar solvent-soluble extract or a non-polar solvent-soluble extract.

In the present invention, the crude extract may be extracted with any solvent conventionally used, preferably water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin, or a mixed solvent thereof .

The crude extract can be obtained by the following steps. Dried rape leaves are blended with water containing 1 to 30 times, preferably 10 to 20 times their volume of purified water, lower alcohols having 1 to 4 carbon atoms, butylene glycol, propylene glycol and glycerin Or more; An extraction temperature of 20 to 100 DEG C, preferably 20 to 60 DEG C in a state where a cooling condenser is installed to prevent the solvent from evaporating; 1 to 30 hours, preferably 3 to 20 hours; 1 to 7 times, preferably 1 to 3 times; Extraction using cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, heat extraction, preferably hot water extraction; The obtained extract is filtered, concentrated under reduced pressure, and freeze-dried or spray-dried to obtain a dried extract.

In the present invention, the polar solvent-soluble extract may be extracted with any polar solvent known in the art, and is preferably extracted with water or n-butanol. The term 'polar solvent-soluble extract' refers to an extract that is extracted from crude extracts using a polar solvent.

In the present invention, the above non-polar solvent-soluble extract can be extracted with any polar solvent known in the art, and is preferably extracted with hexane, methylene chloride or ethyl acetate. The term " nonpolar solvent-soluble extract " refers to an extract that is extracted from crude extract using a non-polar solvent.

The polar solvent and non-polar solvent-soluble extract of the present invention can be obtained by extracting the crude extract of rapeseed, obtained by the above-mentioned process, preferably 2 to 20 times, preferably 10 to 20 times, After water is dispersed, it can be obtained by sequentially adding the same amount of hexane, methylene chloride, ethyl acetate and butanol as water, and fractionating the mixture one to five times, preferably two to four times.

In the present invention, the extract may be contained in an amount of 0.01 to 20% by weight, preferably 0.1 to 10% by weight, based on the total composition. Outside of the above range, the effect is insignificant.

In the present invention, the composition is characterized by having a skin whitening effect by inhibiting tyrosinase activity and inhibiting melanin biosynthesis.

Arbutin used in whitening cosmetics is a chemical synthetic material, whereas the extract of ramie leaves of the present invention is a natural product, so it can be used more safely when used on skin, and since its effect is similar to or higher than that of arbutin at low concentration, arbutin It is more effective because you can see similar whitening efficacy. According to the experimental examples of the present invention, the group treated with the rape leaf extract of the present invention showed inhibition rate of tyrosinase close to that of the positive control group. As a result, the rape leaf extract of the present invention inhibited tyrosinase Thereby inhibiting melanin synthesis and exhibiting a skin whitening effect (Experimental Example 1).

In addition, according to the experimental examples of the present invention, the group treated with ramie leaf extract of the present invention showed a similar inhibitory rate to melanin formation similar to that of the positive control group, and as a result, the ramie leaf extract of the present invention inhibited melanin generation Thereby preventing skin pigmentation and exhibiting skin whitening effect (Experimental Example 3).

In the present invention, the composition may be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizer cream, a hand cream, , A soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

Hereinafter, the present invention will be described more specifically.

The extract of the present invention can be used variously in cosmetics and cleansers having a skin whitening effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, etc., and cleansing, cleansing agents, soaps, treatments, and essences

The cosmetic composition of the present invention may further comprise a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) Alpha-tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl laurate, and the derivatives thereof (palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetate, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic composition of the present invention may contain, in addition to the above-mentioned essential components, other components usually added to the cosmetic composition.

Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearoyl hydroxystearic acid isostearyl, 12-stearoyl stearyl hydroxystearate, 12-stearo And monohydroxystearic acid and esters such as sostearyl.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- , 3,5-triazine, 2- (2-hydroxy Methyl-5-methylphenyl) benzotriazole, and the like.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above components may be blended within the range not to impair the objects and effects of the present invention, but it is preferably 0.01 to 5% by weight, And preferably 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients conventionally used in cosmetic compositions in addition to the above-mentioned compounds, for example, conventional additives such as stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences, and hair cosmetics. Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The ramie leaf extract of the present invention effectively inhibits tyrosinase activity, which is an enzyme essential for the melanin biosynthetic process, and inhibits melanin biosynthesis of melanoma cells, thereby exhibiting a skin whitening effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example  One : Ramie leaves Crude extract  Produce

The dried rhizome leaves (300 g) were placed in 3.6 kg of 50% ethanol, connected with a reflux condenser, extracted with hot water at 40 ° C for 5 hours, filtered through 2500 mesh filter cloth, and filtered through a 0.45 μm filter. Then, the solvent was completely removed from the filtrate by using a rotary evaporator under reduced pressure to obtain 21 g of powdery rape seed extract.

Example  2 : Ramie leaves Nonpolar solvent  Preparation of soluble extract

2-1. Ramie leaves Hexane  Preparation of extract

11 g of the ramie leaf extract obtained in Example 1 was suspended in 220 g of distilled water, and then 220 g of the same amount of hexane was used for shaking and fractionation. After repeating this twice, the hexane layer was concentrated under reduced pressure to obtain 0.3 g (dry weight) of the dried leaf extract of hexane hexane.

2-2. Ramie leaves Methylene chloride  Preparation of extract

To the residue layer of Example 2-1, 400 g of methylene chloride was shaken out and fractionated. Thereafter, the mixture was repeated twice, and the methylene chloride layer was concentrated under reduced pressure to obtain 0.3 g (dry weight) of the methylene chloride soluble extract of rapeseed leaves.

2-3. Ramie leaves  Preparation of ethyl acetate extract

The residue layer of Example 2-2 was subjected to shaking extraction with 200 g of ethyl acetate and then fractionated. Thereafter, the mixture was repeated twice, and the ethyl acetate layer was concentrated under reduced pressure to obtain 0.1 g (dry weight) of ethyl acetate-soluble extract of rape leaf.

Example  3: Ramie leaves  Preparation of polar solvent-soluble extract

3-1. Ramie leaves  n- Butanol  Preparation of extract

The residue layer of Example 2-3 was fractionated after shaking with 200 g of n-butanol saturated with water. After repeating twice, the n-butanol layer was concentrated under reduced pressure to obtain 2.4 g (dry weight) of n-butanol soluble extract of rape leaf.

3-2. Ramie leaves Purified water  Preparation of extract

The residue layer of Example 3-1 was concentrated under reduced pressure to obtain 8.1 g (dry weight) of purified water soluble extract of rape leaf.

Experimental Example  1: inhibition of tyrosinase activity

In this Experimental Example, tyrosinase inhibitory effects of Examples 1 to 3-2 were measured. Inhibitory activity of tyrosinase is generally measured by a spectroscopic method. In this experiment, the inhibitory activity of tyrosinase was measured by Vanni et al. (Annali di Chimica, 80 (35), 1990). Tyrosinase is an enzyme purified from potato or mushroom. In this experiment, mushroom extract was used. 1.0 ml of a 0.1 M potassium phosphate buffer solution (pH 6.8), 1.0 ml of a 0.3 mg / ml L-tyrosine aqueous solution and 0.1 ml of 1250 unit / ml mushroom tyrosinase (Sigma Aldrich, USA) To each sample, 0.2 ml of each sample solution was added, and the mixture was incubated at 37 ° C for 10 minutes. Then, the absorbance of the reaction solution was measured at 480 nm (JASCO V-550, japan) and calculated according to the following formula 1 to determine the enzyme inhibition activity of the sample. As a positive control, 0.2 ml of arbutin (Bio Land) was used at the same concentration as that of the experimental group, and a reaction solution to which no sample was added was used as a control group in the experimental group. The results are shown in Table 1 below.

[Formula 1]

A: absorbance at 480 nm of the reaction solution without addition of the sample

B: Absorbance at 480 nm of the reaction solution to which the sample was added

sample Mushroom tyrosinase inhibition (%) at a concentration of 10 [mu] g / ml Example 1 42.72 Example 2-1 11.58 Example 2-2 19.20 Example 2-3 53.12 Example 3-1 41.46 Example 3-2 16.24 Arbutin 41.51

From the above results, the ethyl acetate of Example 2-3 showed 58.12%, which is the most excellent inhibitory rate of tyrosinase activity. In addition, the crude extract of Example 1 and the n-butanol extract of Example 3-1 showed a similar effect as compared with the positive control group of 41.51% to 42.72% to 41.46%. Therefore, it can be seen that the ramie leaf extract of the present invention is excellent in inhibiting tyrosinase activity.

Experimental Example  2: B16-F1 Melanoma  Cell Cytotoxicity Measurement

In this Experimental Example, the cell survival rate of B16-F1 melanoma cells (CRL-6323, ATCC) was measured using Examples 1 to 3-2 as samples. In this experiment, MTT experiment was performed for cytotoxicity measurement. Specific experimental methods are as follows. B16-F1 melanoma cells, the 10% FBS (Fetal bovine serum, Gibco) was added a DMEM (Dulbecco's modified eagle's medium , Gibco) in 1 × 10 ⁵ density in 6 well plate 5 during the inoculation and day (Nunc) of cell % CO _2, and incubated at 37 ℃. Then, the cells were replaced with fresh DMEM supplemented with 10% FBS, and the samples were treated for different concentrations and cultured for 3 days. After 3 days, the medium was removed and 2 ml of 0.25 mg / ml MTT (3- [4,5-dimethylthiazole-2-yl] -2,5-diphenyltetrazolium bromide) solution was treated and reacted at 37 ° C for 4 hours. Then, 2 ml of DMSO (dimethylsulfoxide) was added to the cells from which the MTT solution had been removed to dissolve the MTT formazan, and the absorbance at 570 nm was measured. The control was experimented without sample and it was used as a control. All experiments were repeated three times and statistically processed to obtain a mean value. The results are shown in Table 2 below.

[Formula 2]

A: Absorbance at 570 nm of the sample

B: Absorbance at 570 nm of the control

Experimental concentration (/ / ml) Cell survival rate (%) Example 1 0.1 100.32 10 101.04 Example 2-1 0.1 102.25 10 106.33 Example 2-2 0.01 93.92 One 95.95 Example 2-3 0.1 100.92 10 100.14 Example 3-1 0.1 99.47 10 99.80 Example 3-2 0.1 99.13 10 99.21 Arbutin 100 101.09 250 97.19

As shown in Table 2, it was found that the cell survival rate of the radish leaf extracts was close to 100%, indicating no cytotoxicity.

Experimental Example  3: B16-F1 Melanoma  Measurement of inhibitory effect on melanin biosynthesis using cells

In this Experimental Example, the melanogenesis inhibitory effect of Examples 1 to 3-2 was measured. The melanogenesis inhibitory effect of B16-F1 melanoma cells was determined by modifying the method of Fukuda et al., J. Soc. Cosmetic Chem., 42 (361), 1991). The number of wells according to all concentrations was prepared in triplicate. Specifically, B16-F1 melanoma cells were inoculated into a 6-well plate at a density of 1 × 10 ⁵ cells in DMEM supplemented with 10% FBS, and cultured at 37 ° C. in 5% CO ₂ for 1 day. Then, the cells were replaced with fresh DMEM supplemented with 10% FBS, and the samples were treated for different concentrations and cultured for 3 days. After 3 days, the cells were washed with 2 ml of PBS (phosphate buffer, saline), treated with 0.5 ml of 1N NaOH, and the cells were collected in a 1.5 ml tube to obtain intracellular melanin. The collected cells were transferred to a 96-well plate and the absorbance at 450 nm was measured to determine the amount of melanin per protein. As a control group, arbutin (Bioland Co.) at a concentration of 100, 250 ㎍ / ml was used, and a reaction solution without sample was used as a control group in the experimental group. The results are shown in Table 3 below.

[Formula 3]

xCVa: cell survival rate of the reaction solution treated with the sample

MCa: [(A-B) / A] x 100

A: Absorbance at 450 nm of the reaction solution without sample treatment

B: Absorbance at 450 nm of the reaction solution treated with the sample

Experimental concentration (/ / ml) Per unit cell
Melanin production inhibition rate (%) Example 1 0.1 38.00 10 50.52 Example 2-1 0.1 3.26 10 8.20 Example 2-2 0.01 3.72 One 7.53 Example 2-3 0.1 46.72 10 58.12 Example 3-1 0.1 31.98 10 42.60 Example 3-2 0.1 12.74 10 26.53 Arbutin 100 34.20 250 48.49

As shown in Table 3, the ethyl acetate of Example 2-3 showed the most excellent inhibitory effect on melanin formation, being 58.12% at 10 占 퐂 / ml. In addition, similar melanin formation inhibitory effect was shown in comparison with the positive control group from 50.52% to 42.60% at 48.49% at 10 μg / ml of the crude extract of Example 1 and the n-butanol extract of Example 3-1. Therefore, it was found that the extract of Rhodiola leaves has an excellent effect on skin whitening by inhibiting melanin production.

Experimental Example  4 : Ramie leaves  Whitening clinical trial of extract

In this Experimental Example, in order to examine in vivo whitening activity in Example 2-3, in which the whitening activity effect in vitro was the best among the rape leaf extracts of Examples 1 to 3-2 for skin whitening, Lotions from the formulation (experimental group) and the comparative formulation (control group) in Table 4 were used to induce artificial pigmentation in 19 to 22 year old women with healthy skin and then double blind and randomized human test methods were applied And the skin whitening effect was evaluated after using the test formulation for 6 weeks.

Raw material Execution Formulation Example 1 Comparative Formulation Example 1 Example 2-3 1.0 - Sitosterol 1.70 1.70 Polyglyceryl 2-oleate 1.50 1.50 Setares 1.2 1.2 cholesterol 1.5 1.5 Dicetyl phosphate 0.4 0.4 Concentrated glycerin 5.0 5.0 Sunflower oil 10.0 10.0 Carboxyvinyl polymer 0.2 0.2 Xanthan gum 0.3 0.3 antiseptic a very small amount a very small amount Spices a very small amount a very small amount Purified water Balance Balance

Experimental Example  5: Visual evaluation

The skin whitening effect was evaluated before the use of formulations and at 1 week, 2 weeks, 4 weeks, and 6 weeks after use. The evaluation criteria were evaluated by two observers using a scale of 1 to 7 points. Table 5 shows the results of visual evaluation by observer 1, and Table 6 shows results of visual evaluation by observer 2. The test group and the control group were repeatedly evaluated according to the viewpoints, and the test results were subjected to the repeated measures analysis to determine the difference between the groups (p <0.05).

<Evaluation Criteria>

0: no pigmentation 1: no or light pigmentation

2: Light pigmentation 3: Light or moderate pigmentation

4: Normal pigmentation 5: Normal or severe pigmentation

6: Severe pigmentation 7: Very severe pigmentation

week N Evaluation value S.E.Mean S.D. Control group 0 weeks 10 6.50 0.1439 0.7647 1 week 10 6.72 0.2141 0.8654 2 weeks 10 5.97 0.1457 0.8341 3 weeks 10 5.82 0.1892 0.8774 4 weeks 10 5.73 0.1573 0.8546 Test group 0 weeks 10 6.31 0.1783 0.7227 1 week 10 6.20 0.1259 0.7699 2 weeks 10 5.32 0.1439 0.7578 3 weeks 10 5.17 0.1578 0.7566 4 weeks 10 4.39 0.1411 0.8347

week N Evaluation value S.E.Mean S.D. Control group 0 weeks 10 6.33 0.1365 0.6413 1 week 10 6.31 0.1423 0.6352 2 weeks 10 5.67 0.1469 0.6465 3 weeks 10 5.35 0.1378 0.6678 4 weeks 10 5.39 0.1245 0.6657 Test group 0 weeks 10 6.42 0.1116 0.6233 1 week 10 6.29 0.1236 0.6198 2 weeks 10 5.31 0.1696 0.6392 3 weeks 10 5.19 0.1345 0.6466 4 weeks 10 4.02 0.1998 0.6250

According to the above Tables 5 and 6, the use of the formulation containing the rape leaf extract of the present invention was significantly improved as compared with the untreated control group.

Formulation Example  1: Flexible lotion ( skin )

As described below, a soft lotion (skin) containing the ragweed extract obtained in Example 2-3 was prepared according to a conventional method. Of the following raw materials, the ramie leaf extract of the present invention is not limited to Examples 2-3 and may be any one selected from Examples 1 to 3-2.

Raw material content Example 2-3 3.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Polyoxyethylene (60) Hardened castor oil 1.00 ethanol 10.0 Triethanolamine 0.1 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

Formulation Example  2: Manufacture of nutrition lotion (lotion)

As described below, a nutritional lotion (lotion) containing the ragweed extract obtained in Example 2-3 was prepared according to a conventional method. Of the following raw materials, the ramie leaf extract of the present invention is not limited to Examples 2-3 and may be any one selected from Examples 1 to 3-2.

Raw material content Example 2-3 1.0 Sitosterol 1.70 Polyglyceryl 2-oleate 1.50 Setares 1.2 cholesterol 1.5 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower oil 10.0 Carboxyvinyl polymer 0.2 Xanthan gum 0.3 antiseptic a very small amount Spices a very small amount Purified water Balance

Formulation Example  3: Manufacture of nutritional cream

As shown below, a nutritive cream containing the ragweed extract obtained in Example 2-3 was prepared according to a conventional method. Of the following raw materials, the ramie leaf extract of the present invention is not limited to Examples 2-3 and may be any one selected from Examples 1 to 3-2.

Raw material content Example 2-3 1.0 Sitosterol 4.0 Polyglyceryl 2-olate 3.0 3.0 Setares-4 2.0 cholesterol 3.0 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower oil 22.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 antiseptic a very small amount Spices a very small amount Purified water Balance

Formulation Example  4: Preparation of essence

As described below, an essence containing the ragweed extract obtained in Example 2-3 was prepared according to a conventional method. Of the following raw materials, the ramie leaf extract of the present invention is not limited to Examples 2-3 and may be any one selected from Examples 1 to 3-2.

Raw material content Example 2-3 1.0 Sitosterol 1.70 Polyglyceryl 2-oleate 1.50 Setares-4 2.0 cholesterol 3.0 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower oil 22.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 antiseptic a very small amount Spices a very small amount Purified water Balance

Formulation Example  5: Manufacture of foundation

As described below, a foundation containing the ragweed extract obtained in Example 2-3 was prepared by a conventional method. Of the following raw materials, the ramie leaf extract of the present invention is not limited to Examples 2-3 and may be any one selected from Examples 1 to 3-2.

Raw material content Example 2-3 1.0 Wax 2.0 Cyclomethicone 2.0 Liquid paraffin 5.0 Squalane 5.0 Stearic acid 2.0 Lipophilic monostearic acid glycerin 3.0 Caprylic / capric triglyceride 4.0 glycerin 4.0 Propylene glycol 3.0 Butylene glycol 3.0 Triethanolamine 1.0 Aluminum magnesium silicate 0.5 Pigment 12 antiseptic a very small amount Spices a very small amount Purified water Balance

INDUSTRIAL APPLICABILITY As described above, according to the present invention, the rape leaf extract of the present invention can be used as a skin whitening cosmetic composition containing a rape leaf extract having a skin whitening effect as an active ingredient.

Claims (8)

A cosmetic composition for skin whitening comprising an extract of ramie leaves as an active ingredient.
The cosmetic composition for skin whitening according to claim 1, wherein the extract is a crude extract, a polar solvent-soluble extract, or a non-polar solvent-soluble extract.
The cosmetic composition for skin whitening according to claim 2, wherein the crude extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin, or a mixed solvent thereof.
The cosmetic composition for skin whitening according to claim 2, wherein the polar solvent-soluble extract is extracted from crude extract with water and n-butanol.
The cosmetic composition for skin whitening according to claim 2, wherein the non-polar solvent-soluble extract is extracted from crude extract with hexane, methylene chloride or ethyl acetate.
The cosmetic composition for skin whitening according to claim 1, wherein the extract is contained in an amount of 0.1 to 10% by weight based on the total composition.
The cosmetic composition for skin whitening according to claim 1, wherein the composition has a skin whitening effect by inhibiting tyrosinase activity and inhibiting melanin biosynthesis.
The composition according to claim 1, wherein the composition is at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizing cream, a hand cream, Wherein the cosmetic composition has one formulation selected from the group consisting of a pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.