KR102124556B1 - Cosmetics for skin whitening including green barely extract and manufacturing method for the same - Google Patents

Abstract

The present invention relates to a skin whitening cosmetic comprising: a skin whitening agent containing a green barley extract; a moisturizing agent; a viscosity-increasing agent; and a surfactant and, more specifically, to a skin whitening cosmetic in which a tyrosinase secretion inhibition rate and a melanin synthesis inhibition rate of the skin whitening cosmetic increase depending on the concentration of the green barley extract.

Description

Skin whitening cosmetics containing green barley extract and manufacturing method thereof {COSMETICS FOR SKIN WHITENING INCLUDING GREEN BARELY EXTRACT AND MANUFACTURING METHOD FOR THE SAME}

The present application relates to skin whitening cosmetics and methods for manufacturing the same.

Human skin is most directly affected by biological, chemical, and physical stimuli because it is directly exposed to the external environment among body tissues. Human skin is largely composed of the epidermal layer, dermal layer, and subcutaneous fat layer. The most basic role of skin is to prevent the evaporation of moisture from our body and to prevent the intrusion of harmful components from the outside. Since these skins are always exposed from external stimuli, they have a variety of defenses. In addition, it can be said that the skin is healthy and clean, which is related to the beauty of a person.

Thanks to these social notions, in order to make the skin look white, or to show the dullness and pigmentation of the skin, interest in skin whitening cosmetics has recently increased. These skin whitening cosmetics can suppress the phenomenon that the skin turns red or black through various principles such as sunscreen, as well as symptomatic therapy to whiten the skin, and to block the synthesis of melanin pigments.

The background paper of this application (Meng, Tian, et al. "Melanin Biosynthesis Inhibitory Activity of a Compound Isolated from Young Green Barley (Hordeum Vulgare L.) in B16 Melanoma Cells." Journal of Natural Medicines, vol. 69, no 3, 2015, pp. 427-431.) is for skin lightening agents using young blue barley powder. The paper discloses that the blue barley powder inhibits melanin synthesis by tricin inside B16 melanoma cells, but fails to recognize that the skin whitening efficacy may be changed when irradiated with light on the blue barley. have.

The present application is to solve the problems of the prior art described above, and an object of the present invention is to provide a skin whitening cosmetic and a method for manufacturing the same.

However, the technical problems to be achieved by the embodiments of the present application are not limited to the technical problems as described above, and other technical problems may exist.

As a technical means for achieving the above technical problem, the first aspect of the present application is a step of forming a green barley extract by mixing green barley and a solvent, forming a skin lightening agent comprising the green barley extract, and the skin whitening agent, Mixing a moisturizer, a viscosity-increasing agent, and a surfactant to form a skin whitening cosmetic, and the skin whitening cosmetic's tyrosinase secretion inhibition rate and melanin synthesis inhibition rate depend on the concentration of the green barley extract. To provide a method for producing skin whitening cosmetics.

According to one embodiment of the present application, the step of forming the green barley extract includes crushing the green barley, extracting the crushed green barley powder by mixing with the solvent and ethanol, and concentrating the extract. However, it is not limited thereto.

According to one embodiment of the present application, the extraction step may be performed at a temperature increase condition of 1°C/min to 20°C/min, but is not limited thereto.

According to one embodiment of the present application, the tyrosinase (tyrosinase) secretion inhibition rate is 40% to 90%, and the melanin synthesis inhibition rate may be 35% to 90%, but is not limited thereto.

According to one embodiment of the present application, the skin whitening agent is buckwheat extract, lemon balm extract, natural extract, niacinamide, arbutin, ethyl ascorbic acid, ascorbyltetraisopalmitate ( Ascorbyl tetraisopalmitate), and combinations thereof, may further include, but are not limited to.

According to one embodiment of the present application, the natural extract is bee odor extract, mulberry extract, green light callus extract, bamboo callus extract, rice cake extract, snow juniper extract, hammock tree extract, red sea ginseng extract, sea cucumber extract, civil incense extract, freshwater seaweed Extracts, and combinations thereof, but are not limited thereto.

According to one embodiment of the present application, the concentration of the green barley extract may be 10 μg/ml to 400 μg/ml, but is not limited thereto.

According to one embodiment of the present application, the solvent may include one selected from the group consisting of hexane, chloroform, ethanol, water, and combinations thereof, but is not limited thereto.

In addition, the second aspect of the present application includes a skin whitening agent, a moisturizing agent, a viscosity-increasing agent, and a surfactant containing a green barley extract, in the skin whitening cosmetic, the inhibition rate of tyrosinase secretion and melanin of the skin whitening cosmetic Synthesis inhibition rate is increased depending on the concentration of the green barley extract, provides a skin whitening cosmetics.

The above-described problem solving means are merely exemplary and should not be construed as limiting the present application. In addition to the exemplary embodiments described above, additional embodiments may exist in the drawings and detailed description of the invention.

According to the above-mentioned problem solving means of the present application, the skin whitening cosmetic prepared by the method according to the present application contains a green barley extract, has low toxicity to cells, and can suppress the production of melanocytes, thereby having an excellent skin whitening effect. have.

In addition, skin whitening cosmetics prepared by the method according to the present application, in addition to the above barley barley extract, buckwheat extract, lemon balm extract, bee odor extract, mulberry extract, green light callus extract, bamboo callus extract, rice cake bottle extract, snow juniper extract, impurity tree extract , Since it contains various natural extracts such as red sea ginseng extract, sea cucumber extract, civil scent extract, and freshwater seaweed extract, it has less irritation to the skin as well as skin whitening, has excellent skin soothing effect, and is effective in improving skin trouble.

In addition, the skin whitening cosmetics prepared by the method according to the present application can suppress the evaporation of moisture in the skin surface cells, thereby suppressing the skin from becoming cloudy.

In addition, the skin whitening cosmetics according to the present application contain a large amount of powerful antioxidants such as beta carotene, vitamin C, vitamin E, and SOD of green barley extract to maintain the gloss of the skin when applied to the skin, and aging, wrinkles, spots, and sebum Etc. can be prevented.

Moreover, in the manufacturing method of skin whitening cosmetics according to the present application, since the step of preparing the green barley extract gradually changes the temperature and the wavelength of the irradiated light, the skin whitening cosmetics can obtain a higher content of ingredients effective for skin whitening, , The process cost of the manufacturing method may be reduced.

However, the effects obtainable herein are not limited to the above-described effects, and other effects may exist.

1 is a graph showing tyrosinase inhibitory activity of green barley extract according to an embodiment of the present application.
2 is a graph showing the cytotoxicity of green barley extract according to an embodiment of the present application.
3 is a graph showing the cytotoxicity of green barley extract according to an embodiment of the present application.
4 is a graph showing the melanin synthesis inhibitory ability of green barley extract according to an embodiment of the present application.
Figure 5 is a graph showing the melanin synthesis inhibitory ability of green barley extract according to an embodiment of the present application.

Hereinafter, embodiments of the present application will be described in detail with reference to the accompanying drawings so that those skilled in the art to which the present application pertains may easily practice.

However, the present application may be implemented in various different forms and is not limited to the embodiments described herein. In addition, in order to clearly describe the present application in the drawings, parts irrelevant to the description are omitted, and like reference numerals are assigned to similar parts throughout the specification.

Throughout this specification, when a part is "connected" to another part, this includes not only "directly connected" but also "electrically connected" with another element in between. do.

Throughout the present specification, when a member is positioned on another member “on”, “on the top”, “top”, “bottom”, “bottom”, “bottom”, this means that one member is attached to another member. This includes cases where there is another member between the two members as well as when in contact.

Throughout this specification, when a part “includes” a certain component, it means that the component may further include other components, not to exclude other components, unless otherwise stated.

As used herein, the terms “about”, “substantially”, and the like are used in or near the numerical values when manufacturing and substance tolerances unique to the stated meanings are presented, to aid understanding of the present application Hazards are used to prevent unreasonable abuse by unscrupulous infringers of the disclosures that are either accurate or absolute. In addition, throughout the present specification, "step of" or "step of" does not mean "step for".

Throughout the present specification, the term “combination of these” included in the expression of the marki form means one or more mixtures or combinations selected from the group consisting of the components described in the expression of the marki form, the component. It means to include one or more selected from the group consisting of.

Throughout this specification, the description of “A and/or B” means “A or B, or A and B”.

Hereinafter, the skin whitening cosmetic and its manufacturing method of the present application will be described in detail with reference to embodiments and examples and drawings. However, the present application is not limited to these embodiments and examples and drawings.

As a technical means for achieving the above technical problem, the first aspect of the present application is a step of forming a green barley extract by mixing green barley and a solvent, forming a skin lightening agent comprising the green barley extract, and the skin whitening agent, Mixing a moisturizer, a viscosity-increasing agent, and a surfactant to form a skin whitening cosmetic, and the skin whitening cosmetic's tyrosinase secretion inhibition rate and melanin synthesis inhibition rate depend on the concentration of the green barley extract. To provide a method for producing skin whitening cosmetics.

The skin whitening cosmetics according to the present application can reduce the pores of the skin, make the skin white, as well as adjust the irregular skin color regularly, reduce the dullness of the skin, etc., which can suppress the synthesis of melanin in the skin. it means.

These skin whitening cosmetics are not only skin whitening agents such as niacinamide, arbutin, ethyl ascorbic acid, ascorbyl tetraisopalmitate, but also a skin conditioner for moisturizing the skin, The moisturizing agent (humectant) that maintains the moisture of the cosmetic, a viscosity increasing agent that increases the viscosity so that the skin whitening cosmetic does not flow off the skin, a surfactant for mixing the ingredients of the skin whitening cosmetic, the pH of the skin whitening cosmetic PH adjusting agent to adjust to have weak acidity or weak alkalinity, chelating agent to prevent traces of metal ions from entering the skin whitening cosmetics, fragrance to make the cosmetics have a fragrance, and the cosmetics to include all the ingredients And a mixed solvent capable of performing various functions such as extraction of an active ingredient.

In this regard, the solvent for causing the cosmetic product to have a liquid form generally means a substance such as water or ethanol, and is referred to herein as a “mixed solvent”. Unless otherwise specified, the term "solvent" as used herein refers to the substance used to form the extract, and the term "concentration of extract" refers to the value of the mass of the active ingredient obtained in the extract divided by the volume of the solvent. it means.

Green barley according to the present application is a kind of barley, and means a barley having a blue-colored light, not a yellow color. In general, barley is known to contain various bioactive substances such as beta carotene (β-carotene), vitamin C, vitamin E, and superoxide dismutase (SOD). In this regard, green barley, like common barley, contains various bioactive substances, and can be used for various purposes, such as energy activation in the body, protein synthesis, digestive action, skin moisture, and anti-aging.

According to one embodiment of the present application, the green barley may include green barley grown for 1 to 6 months, but is not limited thereto.

According to one embodiment of the present application, the green barley extract may include polyphenols and/or flavonoids as active ingredients, but is not limited thereto.

A polyphenol according to the present application means a substance in which a part of sugars produced when a plant is photosynthesized is converted. The polyphenol may perform strong antioxidant action by including two or more hydroxyl groups (-OH). The phenol of the green barley extract may contain feruraic acid to inhibit the production of melanin pigments. In addition, the flavonoid according to the present application is a type of polyphenol, and refers to a substance associated with plant pigments.

When the skin is exposed to ultraviolet light for a long time, melanin may be generated in the skin. Specifically, skin keratinocytes exposed to ultraviolet rays secrete melanocyte stimulating hormone, and the melanocyte stimulating hormone binds to the melanocortin 1 receptor and secretes enzymes such as tyrosinase and TYRP1. The tyrosinase serves as a catalyst for transforming tyrosine into L-DOPA (L-3,4-dihydroxyl-L-phenylalanine), and transforming L-DOPA into L-dopaquinone, L-Dopaquinone can function as a precursor of melanin. On the other hand, melanin produced from B16-F10 melanocyte cells present in the base layer of skin cells may be delivered to the surface of the skin by the L-dopaquinone.

In this regard, the color of the skin is determined by the molecular structure and color of the melanin, and the melanin serves to inhibit the penetration of the ultraviolet light into the dermal layer for about 2 months. However, the melanin does not suppress 100% of ultraviolet rays, and if the ultraviolet rays are continuously received, the color of the skin may become semi-permanently dark, and various problems such as aging of the skin or pigmentation may occur.

As will be described later, the skin whitening cosmetic product according to the present application is intended to suppress the synthesis of the melanin and not to stimulate the skin cells.

According to the exemplary embodiment of the present application, the inhibition rate of tyrosinase secretion and the inhibition of melanin synthesis of the skin whitening cosmetics may be increased depending on the concentration of the barley extract, but is not limited thereto.

According to one embodiment of the present application, the tyrosinase secretion inhibition rate of the skin whitening cosmetic is 40% to 90%, and the melanin synthesis inhibition rate may be 35% to 90%, but is not limited thereto.

For example, the tyrosinase secretion inhibition rate is about 40% to about 90%, about 45% to about 90%, about 50% to about 90%, about 55% to about 90%, about 60% to about 90% , About 65% to about 90%, about 70% to about 90%, about 75% to about 90%, about 80% to about 90%, about 85% to about 90%, about 40% to about 45%, about 40% to about 50%, about 40% to about 55%, about 40% to about 60%, about 40% to about 65%, about 40% to about 70%, about 40% to about 75%, about 40% To about 80%, about 40% to about 85%, about 45% to about 85%, about 50% to about 80%, about 55% to about 75%, about 60% to about 70%, or about 65% days However, it is not limited thereto.

In addition, the melanin synthesis inhibition rate is about 35% to about 90%, about 40% to about 90%, about 45% to about 90%, about 50% to about 90%, about 55% to about 90%, about 60% To about 90%, about 65% to about 90%, about 70% to about 90%, about 75% to about 90%, about 80% to about 90%, about 85% to about 90%, about 35% to about 40%, about 35% to about 45%, about 35% to about 50%, about 35% to about 55%, about 35% to about 60%, about 35% to about 65%, about 35% to about 70% , About 35% to about 75%, about 35% to about 80%, about 35% to about 85%, about 40% to about 85%, about 45% to about 80%, about 50% to about 75%, about 55% to about 70%, or about 60% to about 65%, but is not limited thereto.

The inhibition rate of tyrosinase secretion according to the present application means the degree of inhibiting the production of the L-DOPA and the production of the melanin. In addition, the inhibition rate of melanin synthesis according to the present application means the degree of inhibiting the synthesis of melanin in the skin, but may not have a form consistent with the inhibition rate of tyrosinase secretion.

According to one embodiment of the present application, the barley barley extract may have a cell survival rate of 20% to 100%, but is not limited thereto.

Cell viability according to the present application means the degree to which the mitochondria of the cells can survive by the barley barley extract. Specifically, the dehydrogenase in the cell's mitochondria is yellow water-soluble tetrazoliumsalt[3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide] (MTT) as a water-insoluble dark purple MTT formazan crystal When reduced, the cell viability can be analyzed through the degree to which the crystal absorbs light having a wavelength of 500 nm to 600 nm. Through the cell viability, it can be confirmed how much toxicity a specific substance has to the cell.

First, green barley and a solvent are mixed to form a green barley extract.

According to one embodiment of the present application, the step of forming the green barley extract may include grinding the green barley, extracting the ground green barley powder by mixing with the solvent and ethanol, and fractionating the extract. However, it is not limited thereto.

According to one embodiment of the present application, the step of forming the green barley extract may further include a step of concentrating the extracted material, but is not limited thereto.

According to one embodiment of the present application, the green barley powder may include crushed green barley leaves, roots, stems, fruits, or seeds, but is not limited thereto.

In this regard, the mass ratio of the powder to the mass of the solvent and ethanol may be 15 to 25 times, but is not limited thereto.

The substance used when extracting the powder, such as a mixed solution of the solvent and the ethanol, may be referred to as an extraction solution.

According to one embodiment of the present application, the extraction solution may be pure ethanol, or a mixed solution of 30% by volume or more and less than 100% by volume, and 0% by volume or more and 70% by volume or less of the solvent, but is not limited thereto.

According to one embodiment of the present application, the solvent may include one selected from the group consisting of hexane, chloroform, ethanol, water, and combinations thereof, but is not limited thereto.

According to one embodiment of the present application, the extraction step may be performed at a temperature increase condition of 1°C/min to 20°C/min, but is not limited thereto.

According to the exemplary embodiment of the present application, the extracting step may include one selected from the group consisting of reflux extraction, stationary extraction, ultrasonic extraction, high temperature pressure extraction, low temperature high pressure extraction, and combinations thereof, but is not limited thereto. no.

When the extracting step is performed by a reflux extraction process, the solvent in which the active ingredient in the powder is dissolved flows downward by gravity, and when the solvent is vaporized, the solvent is liquefied in the process of moving to the rising part to extract the active ingredient from the powder. It is known that the energy saving effect is great, such as being able to extract.

In this regard, the extracting step may include stirring conditions and ultrasonic irradiation conditions. The stirring and irradiation of the ultrasonic wave may improve the extraction efficiency of active ingredients such as polyphenols from the powder.

According to the exemplary embodiment of the present application, the stirring may be performed at a speed of 10 rpm to 300 rpm, and the ultrasonic waves may have a frequency of 10 kHz to 1 MHz, but are not limited thereto.

According to one embodiment of the present application, the extraction step may be performed at a temperature condition of 60°C to 100°C through the temperature increase condition, but is not limited thereto.

The extraction step performed without going through the temperature rising condition, that is, when the temperature of the solvent and the ethanol is heated to be located within a range of 60° C. to 100° C., and then mixed with the green barley powder to extract reflux, the active ingredient of the green barley Skin whitening performance may be reduced due to reduced extraction.

The temperature increase condition according to the present application means that the degree of temperature rise with time is defined. In the case of extracting the components in the substance under the elevated temperature condition, the components are slowly extracted through the cell wall of the substance and may be extracted with higher purity when extracting the components at a constant temperature.

The step of fractionation according to the present application means the step of separating after mixing the reflux extract with the solvent.

According to one embodiment of the present application, the concentration of the green barley extract may be 10 μg/ml to 400 μg/ml, but is not limited thereto. For example, the concentration of the green barley extract is about 10 μg/ml to about 400 μg/ml, about 25 μg/ml to about 400 μg/ml, about 50 μg/ml to about 400 μg/ml, about 75 μg/ ml to about 400 μg/ml, about 100 μg/ml to about 400 μg/ml, about 150 μg/ml to about 400 μg/ml, about 200 μg/ml to about 400 μg/ml, about 250 μg/ml to About 400 μg/ml, about 300 μg/ml to about 400 μg/ml, about 350 μg/ml to about 400 μg/ml, about 10 μg/ml to about 25 μg/ml, about 10 μg/ml to about 50 μg/ml, about 10 μg/ml to about 75 μg/ml, about 10 μg/ml to about 100 μg/ml, about 10 μg/ml to about 150 μg/ml, about 10 μg/ml to about 200 μg/ ml, about 10 μg/ml to about 250 μg/ml, about 10 μg/ml to about 300 μg/ml, about 10 μg/ml to about 350 μg/ml, about 25 μg/ml to about 350 μg/ml, About 50 μg/ml to about 300 μg/ml, about 75 μg/ml to about 250 μg/ml, about 100 μg/ml to about 200 μg/ml, or about 150 μg/ml, but is not limited to no.

In this regard, the concentration of the green barley extract means the concentration of the concentrated green barley extract or the green barley extract before concentration, and does not mean the extent to which the skin whitening agent described later includes the green barley extract.

As will be described later, the whitening performance of the skin whitening cosmetic may vary depending on the type of solvent used when extracting the green barley extract and the concentration of the green barley extract.

Subsequently, a skin whitening agent containing the green barley extract is formed.

According to one embodiment of the present application, the skin whitening agent is buckwheat extract, lemon balm extract, natural extract, niacinamide, arbutin, ethyl ascorbic acid, ascorbyltetraisopalmitate ( Ascorbyl tetraisopalmitate), and combinations thereof, may further include, but are not limited to. In this regard, the skin whitening agent may include substances that help skin whitening, such as decolorizing the color of pre-synthesized melanin or inhibiting tyrosinase secretion to suppress the production of melanin itself.

Niacin amide according to the present application means vitamin B3, and may have various effects, such as shrinking pores, adjusting unbalanced skin tone, and improving aging of the skin, and arbutin can inhibit melanin's pigment activation and skin pigmentation. Ethyl ascorbyl ether and ascorbyl tetraisopalmitate are derivatives of ascorbic acid (vitamin C), and unlike ascorbic acid, can be dissolved in various organic solvents and stably pigmented even when applied to weakly acidic skin. It means a substance that has an excellent skin whitening effect, such as resolving it.

According to one embodiment of the present application, the buckwheat extract, the lemon balm extract, and the natural extract are selected from the group consisting of reflux extraction, stationary extraction, ultrasonic extraction, high temperature pressure extraction, low temperature high pressure extraction, and combinations thereof. It may be formed by, but is not limited to.

According to one embodiment of the present application, the skin whitening agent may include the green barley extract as 0.1 parts by weight to 10 parts by weight based on 100 parts by weight of the skin whitening agent, but is not limited thereto. For example, with respect to 100 parts by weight of the skin whitening agent, the skin whitening agent may contain about 0.1 parts by weight to about 10 parts by weight, about 1 part by weight to about 10 parts by weight, about 2 parts by weight to about 10 parts by weight, About 3 parts to about 10 parts, about 4 parts to about 10 parts, about 5 parts to about 10 parts, about 6 parts to about 10 parts, about 7 parts to about 10 parts, About 8 parts to about 10 parts, about 9 parts to about 10 parts, about 0.1 parts to about 1 part, about 0.1 parts to about 2 parts, about 0.1 parts to about 3 parts, About 0.1 parts to about 4 parts by weight, about 0.1 parts to about 5 parts, about 0.1 parts to about 6 parts, about 0.1 parts to about 7 parts, about 0.1 parts to about 8 parts, About 0.1 parts by weight to about 9 parts by weight, about 1 part by weight to about 9 parts by weight, about 2 parts by weight to about 8 parts by weight, about 3 parts by weight to about 7 parts by weight, about 4 parts by weight to about 6 parts by weight. Or about 5 parts by weight, but is not limited thereto.

In this regard, the remaining amount of the skin lightening agent, that is, 90 parts by weight to 99.9 parts by weight of natural extract, niacinamide, arbutin, ethyl ascorbic acid, ascorbyltetraisopalmitate tetraisopalmitate), and combinations thereof, or a solvent for mixing the skin whitening agent with other substances, but are not limited thereto.

According to one embodiment of the present application, the natural extract is bee odor extract, mulberry extract, green light callus extract, bamboo callus extract, rice cake extract, snow juniper extract, hammock tree extract, red sea ginseng extract, sea cucumber extract, civil incense extract, freshwater seaweed Extracts, and combinations thereof, but are not limited thereto.

The mulberry extract according to the present application can inhibit the activity of the tyrosinase, including kazinol F (kazinol F), in addition to the mulberry extract, bee odor extract, green light callus extract, bamboo callus extract, rice cake extract, snowwood extract , Ham flower extract, red sea ginseng extract, sea cucumber extract, civil incense extract, and/or freshwater seaweed extract may have the effect of skin whitening.

The buckwheat extract itself according to the present application does not function as a skin whitening agent, but the buckwheat extract may inhibit aging of cells by performing an antioxidant action, and the lemon balm extract according to the present application may function as a skin whitening agent.

According to the exemplary embodiment of the present application, the green barley extract, the natural extract, the buckwheat extract, and the lemon balm extract may be prepared using all parts of plants, animals, or organisms as raw materials. For example, the plant extract may be extracted from a site selected from the group consisting of roots, stems, leaves, flowers, fruits, seeds and combinations of the plants.

In this regard, depending on various factors such as scientific name, varieties, and growth environment, a substance contained in an organism may be different. Specifically, the green barley is Secale cereale of Hordeum vulgare . L may include, the lemon balm may include Melissa officinalis , the buckwheat may include Fagopyrum esculentum , the mulberry may include Broussonetia kazinoki , and the hummingbird may include Aster koraiensis The Hibiscus syriacus may include Hibiscus syriacus , the bamboo may include Sasa coreana Nakai , and the Mochibuta may include Salix hallaisanensist, and the snowwood tree may include Juniperus chinensis var. sargentii , the impregnated tree may include Magnolia sieboldii , the red sea ginseng may include Stichopus japonicus Red or Stichopus japonicus Orange , and the civil scent may include Inula helenium , the freshwater seaweed May include, but is not limited to, Prasiola japonic . In addition, the sea cucumber may include organisms of the genus Holothuroidea , but is not limited thereto.

According to one embodiment of the present application, with respect to 100 parts by weight of the skin whitening agent, each of the skin whitening agent independently 0.01 parts by weight to 1 part by weight of the extract of antler odor, 0.01 part by weight to 1 part by weight of the mulberry extract, 0.01 part by weight of the green light callus extract To 1 part by weight, from 0.01 parts by weight to 1 part by weight of bamboo callus extract, from 0.01 parts by weight to 1 part by weight of rice cake, extract from 0.01 parts by weight to 1 part by weight of snow stalk extract, from 0.01 parts by weight to 1 part by weight of extract from imperial tree, red sea ginseng Extract 0.01 part by weight to 1 part by weight, sea cucumber extract 0.01 part by weight to 1 part by weight, civil scent extract 0.01 part by weight to 1 part by weight, freshwater seaweed extract 0.01 part by weight to 1 part by weight, buckwheat extract 0.01 part by weight to 1 part by weight , And 0.1 parts by weight to 5 parts by weight of lemon balm extract, but is not limited thereto.

According to one embodiment of the present application, the step of forming the skin whitening agent may be performed before or after performing the step of concentrating the green barley extract, but is not limited thereto.

For example, the skin whitening agent is the buckwheat extract, the lemon balm extract, the natural extract, the niacinamide, the arbutin, the ethyl ascorbic acid in the extracted barley barley extract solution , And adding the ascorbyl tetraisopalmitate, or re-dissolving the concentrated green barley extract in the solvent, the natural extract, the buckwheat extract, the lemon balm extract, the niacin amide, the arbutin, It can be obtained by preparing a solution mixed with the ethyl ascorbyl ether and the ascorbyl tetraisopalmitate, and then removing the solvent from the solution, such as filtering the solution.

According to one embodiment of the present application, the method for manufacturing the skin whitening cosmetic product comprises the barley barley extract, the natural extract, the buckwheat extract, and the lemon balm extract, Lactobacillus acidophilus , Lactobacillus sake ( Lactobacillus sakei ), Lactobacillus rhamnosus , Lactobacillus plantarum , Lactobacillus casei , Lactobacillus paracasei , and combinations of these selected from Lactobacillus Paracasei It may further include the step of fermentation by lactic acid bacteria containing it, but is not limited thereto.

The lactic acid bacteria according to the present application may provide sufficient nutrient supply to the skin, improve blood circulation, and facilitate metabolism, so that the skin whitening agent can help suppress the production of melanin.

According to one embodiment of the present application, the method for manufacturing the skin whitening cosmetic may further include the step of photo-treating the green barley extract, the natural extract, the buckwheat extract, and the lemon balm extract as ultraviolet light, visible light, or infrared light. However, it is not limited thereto. In this regard, the skin whitening effect may be improved when the green barley powder or the lemon balm powder is photo-treated or the extract is photo-treated.

According to one embodiment of the present application, the wavelength of light used in the photo-processing step may be 200 nm to 900 nm, but is not limited thereto. For example, the wavelength of the light is about 200 nm to about 900 nm, about 250 nm to about 900 nm, about 300 nm to about 900 nm, about 350 nm to about 900 nm, about 400 nm to about 900 nm, about 450 nm to about 900 nm, about 500 nm to about 900 nm, about 550 nm to about 900 nm, about 600 nm to about 900 nm, about 650 nm to about 900 nm, about 700 nm to about 900 nm, about 750 nm To about 900 nm, about 800 nm to about 900 nm, about 850 nm to about 900 nm, about 200 nm to about 250 nm, about 200 nm to about 300 nm, about 200 nm to about 350 nm, about 200 nm to about 400 nm, about 200 nm to about 450 nm, about 200 nm to about 500 nm, about 200 nm to about 550 nm, about 200 nm to about 600 nm, about 200 nm to about 650 nm, about 200 nm to about 700 nm , About 200 nm to about 750 nm, about 200 nm to about 800 nm, about 200 nm to about 850 nm, about 250 nm to about 850 nm, about 300 nm to about 800 nm, about 350 nm to about 750 nm, about 400 nm to about 700 nm, about 450 nm to about 650 nm, about 500 nm to about 600 nm, or about 550 nm, but is not limited thereto.

According to the exemplary embodiment of the present application, the wavelength of the light may be changed at a rate of 1 nm/min to 100 nm/min, but is not limited thereto.

For example, the wavelength of the light is about 1 nm/min to about 100 nm/min, about 5 nm/min to about 100 nm/min, about 10 nm/min to about 100 nm/min, about 20 nm/min To about 100 nm/min, about 30 nm/min to about 100 nm/min, about 40 nm/min to about 100 nm/min, about 50 nm/min to about 100 nm/min, about 60 nm/min to about 100 nm/min, about 70 nm/min to about 100 nm/min, about 80 nm/min to about 100 nm/min, about 90 nm/min to about 100 nm/min, about 1 nm/min to about 5 nm /min, about 1 nm/min to about 10 nm/min, about 1 nm/min to about 20 nm/min, about 1 nm/min to about 30 nm/min, about 1 nm/min to about 40 nm/min , About 1 nm/min to about 50 nm/min, about 1 nm/min to about 60 nm/min, about 1 nm/min to about 70 nm/min, about 1 nm/min to about 80 nm/min, about 1 nm/min to about 90 nm/min, about 5 nm/min to about 90 nm/min, about 10 nm/min to about 80 nm/min, about 20 nm/min to about 70 nm/min, about 30 nm /min to about 60 nm/min, or from about 40 nm/min to about 50 nm/min, but is not limited thereto.

In the photo-processing step, when the wavelength of the light was changed at a constant speed, unlike the case where the wavelength of the light was kept constant, it was confirmed that more active ingredients were extracted.

In this regard, a condition for changing the wavelength of light at a constant speed in the photo-processing step may be referred to as a wavelength shift condition and a condition for keeping the wavelength of light constant.

According to one embodiment of the present application, the step of fermentation by the lactic acid bacteria and the step of photo-processing may be performed before or after preparing the green barley extract, or before or after preparing the skin lightening agent, but are not limited thereto. no.

Subsequently, the skin whitening agent, moisturizing agent, viscosity increasing agent, and surfactant are mixed to form a skin whitening cosmetic.

The surfactant according to the present application is a detergent for removing contaminants in the skin whitening cosmetics, a solubilizer that dissolves a small amount of oil in a solvent transparently, an emulsifier that mixes oil and a solvent well, forms a foam, and the effectiveness of other surfactants It may include, for example, a foam accelerator to increase, but is not limited thereto.

According to one embodiment of the present application, the humectant may include glycerin, but is not limited thereto.

According to one embodiment of the present application, the skin whitening cosmetic comprises a xanthan gum, a hydroxy ethyl cellulose, and a viscosity-increasing agent comprising one selected from the group consisting of cocamidopropyl betaine, sodium chloride, lauryl Surfactants including hydroxy sultaine, decyl glucoside, and octyldodeceth-16 may be further included, but are not limited thereto.

Xanthan gum according to the present application refers to a high molecular weight heteropolysaccharide gum obtained by purely culturing and presenting carbohydrates using soybean germ disease ( Xanthomonas campestris ). The xanthan gum not only increases the viscosity of the skin whitening cosmetic, but also suppresses the separation of components on the skin whitening cosmetic and serves to supply moisture to the skin.

Hydroxy ethyl cellulose according to the present application means a modified cellulose polymer component having a hydroxy ethyl side chain structure, and is known to have excellent salt resistance and pH stability.

Cocamidopropyl betaine according to the present application means an amphoteric surfactant obtained by neutralizing fatty acids obtained from coconut oil with dimethylaminopropylamine, and may be used as a foam accelerator or a viscosity-increasing agent.

Sodium chloride according to the present application is to increase the viscosity of the surfactant, it may further provide an acidity control and antiseptic function.

The lauryl hydroxy sultaine according to the present application is an amphoteric surfactant, and may further perform a role of controlling the viscosity of the skin whitening cosmetics.

The decyl glucoside according to the present application is a component obtained by a condensation reaction between decyl alcohol and glucose, and is a nonionic vegetable surfactant extracted from corn, coconut, and palm cornel oil. The decyl glucoside provides skin moisturizing and can be used as a skin conditioner as it has little irritation to the skin.

Octyldodeceth-16 according to the present application is a type of surfactant which is a polyethylene glycol ether of octyldodecanol. The octyldodeceth-16 has excellent skin affinity and can help the skin absorb the active ingredient.

In addition, the second aspect of the present application includes a skin whitening agent, a moisturizing agent, a viscosity-increasing agent, and a surfactant containing a green barley extract, in the skin whitening cosmetic, the inhibition rate of tyrosinase secretion and melanin of the skin whitening cosmetic Synthesis inhibition rate is increased depending on the concentration of the green barley extract, provides a skin whitening cosmetics.

For skin whitening cosmetics according to the second aspect of the present application, detailed descriptions of parts overlapping with the first aspect of the present application have been omitted, but the description of the first aspect of the present application may be omitted even if the description is omitted. The same can be applied to.

According to one embodiment of the present application, the skin whitening cosmetic may have a form selected from the group consisting of gel, cream, ointment, lotion, pack, and combinations thereof, but is not limited thereto.

The present invention will be described in more detail through the following examples, but the following examples are for illustrative purposes only and are not intended to limit the scope of the present application.

[Example 1]

Leaves and stems of green barley ( Hordeum vulgare ) that were fermented by Lactobacillus acidophilus and irradiated for 1 hour with light were crushed. Subsequently, 200 g of the powder was added to the extraction flask, 2 L of ethanol and 2 L of hexane were mixed, the temperature was increased by 5°C per minute, and reflux was extracted twice at a final temperature of 80°C for 2 hours.

In this regard, the initial wavelength of the light was 500 nm, but decreased by 10 nm per minute, and finally became 300 nm, and 1 hour of light irradiation was from 500 nm to 300 nm and 300 nm wavelength light. Including the treatment time, the reflux extraction process was performed while stirring at a speed of 60 rpm and irradiating 50 kHz ultrasonic waves.

Subsequently, the reflux extract was filtered using a filter paper (Whatman No. 2, GE Health care, UK), concentrated under reduced pressure to obtain an extract concentrate, and then suspended with 100 ml of distilled water.

Then, the suspension and 100 ml of hexane were mixed and fractionated over 3 hours. After repeating the fractionation process 3 times, it was kept refrigerated at 4°C.

[Example 2]

It was the same as in Example 1, but chloroform was used instead of hexane.

[Example 3]

The same as in Example 1, but purified water was used instead of hexane.

[Example 4]

The same as in Example 1, but the fractionation step was omitted.

[Example 5]

To the green barley extract obtained through the process of Example 1, lemon balm ( Melissa officinalis ) extract, buckwheat ( Fagopyrum esculentum ) extract, mulberry tree ( Broussonetia kazinoki ) extract, bee odor ( Aster koraiensis ) extract, Hibiscus syriacus extract, god Sasa coreana Nakai extract, Salix hallaisanensist extract, Snow tree ( Juniperus chinensis var. sargentii ) extract, Magnolia sieboldii extract, Red sea ginseng ( Stichopus japonicus Red or Stichopus japonicus Orange ) extract, Civil incense ( Skin whitening agents were prepared by mixing Inula helenium ) extract, freshwater seaweed ( Prasiola japonic ) extract, and sea cucumber ( Holothuroidea ) extract, niacin amide, arbutin, ethyl ascorbyl ether, ascorbyltetraisopalmitate, and purified water.

At this time, with respect to 1 g of the skin whitening agent, 100 mg of the green barley extract, 10 mg of the lemon balm extract, 10 mg of buckwheat extract, 10 mg of mulberry extract, 10 mg of green light extract, 10 mg of Shinidae extract, 10 mg of pickle butter extract, 10 mg of snowwood extract, 10 mg of imperial tree extract, 10 mg of red sea ginseng extract, 10 mg of geoscent extract, 10 mg of freshwater seaweed extract, 10 mg of sea cucumber extract, 20 mg of niacin amide, 20 mg of arbutin, 10 mg of ethyl ascorbyltel, And 20 mg of ascorbyltetraisopalmitate and the balance of purified water. Subsequently, glycerol, xanthan gum, hydroxy ethyl, cellulose, cocamidopropyl betaine, sodium chloride, lauryl hydroxy sultaine, decylglucoside, octyldodeceth-16, purified water, and ethanol are added to the skin whitening agent. Whitening cosmetics were prepared.

[Comparative Example 1]

The same as in Example 1, but without heating conditions, after mixing the green barley powder in a mixture of 2 L of ethanol and 2 L of hexane at 80° C., reflux extraction was performed to prepare a green barley extract.

[Comparative Example 2]

The same as in Example 1, but did not perform the fermentation process by Lactobacillus acidophilus.

[Comparative Example 3]

The same as in Example 1, but did not perform the light treatment process by ultraviolet light.

[Comparative Example 4]

The same as in Example 1, but did not perform the fermentation process by Lactobacillus acidophilus and the light treatment process by UV light.

[Comparative Example 5]

The same as in Example 1, the lemon balm extract was prepared by irradiating UV light having a wavelength of 300 nm for 1 hour.

[Comparative Example 6]

Same as in Example 1, but was not stirred in the reflux extraction process.

[Comparative Example 7]

The same as in Example 1, but ultrasonic waves were not irradiated in the reflux extraction process.

[Comparative Example 8]

Same as in Example 5, a skin whitening cosmetic product containing only the green barley extract was prepared.

[Comparative Example 9]

The same as in Example 5, the skin whitening cosmetic product that does not contain the green barley extract was prepared.

[Comparative Example 10]

The same as in Example 5, the green barley extract of Comparative Example 4 was used as the green barley extract.

[Experimental Example 1]

The polyphenol and flavonoid contents of the green barley extracts of Examples 1 to 4 were analyzed through the Folin-Denis method and Folin-Ciocalteu, and the contents are shown in Table 1 below.

[Table 1]

Referring to Table 1, it can be seen that the polyphenol content in the green barley extract was the highest when fractionated with chloroform, and the flavonoid content was the highest when fractionated with hexane.

[Experimental Example 2]

1 is a graph showing tyrosinase inhibitory activity of green barley extracts according to Examples 1 to 4 above.

Referring to FIG. 1, it can be seen that when the green barley extract is fractionated, the tyrosinase inhibitory effect is increased according to the concentration of the green barley extract.

2 and 3 is a graph showing the cytotoxicity of green barley extract according to the above embodiment. Specifically, FIG. 2 is the green barley extract of Example 1, and FIG. 3 is the green barley extract of Example 2 above.

2 and 3, it can be seen that the higher the concentration of the green barley extract is 100 μg/ml or less, the more toxic the cells become.

4 and 5 is a graph showing the melanin synthesis inhibitory ability of green barley extract according to an embodiment of the present application. Specifically, FIG. 4 is the green barley extract of Example 1, and FIG. 5 is the green barley extract of Example 2 above.

4 and 5, when the concentration of the green barley extract of Example 1 is in the range of 50 μg/ml or less, or as the concentration of green barley extract of Example 2 increases, it can be confirmed that the biosynthesis amount of melanin is inhibited. have.

1 to 5, when the concentration of the green barley extract of Example 1 is 0.01 μg/ml to 50 μg/ml, the overall skin whitening performance is excellent, and the green barley extract of Example 2 has a concentration of 0.01. When it is from μg/ml to 100 μg/ml, it can be seen that the overall skin whitening performance is excellent.

[Experimental Example 3]

Table 2 below shows the performance of the green barley extract of Example 1 and Comparative Examples 2 to 5.

[Table 2]

Referring to Table 2, when the temperature is not raised and the green barley extract is prepared under certain conditions (Comparative Example 1), when the fermentation process is not performed (Comparative Example 2), when the light treatment process by ultraviolet light is not performed (Comparative Example 3), when the fermentation process and the light treatment process are not performed (Comparative Example 4), when the wavelength is maintained during the light treatment (Comparative Example 5), or when agitation or ultrasonic irradiation is not performed (Comparative Example 6 or 7) ) Can be confirmed that the extraction amount of the polyphenol and flavonoid is less than when the temperature treatment, the fermentation process, and the light treatment process under the wavelength shift condition are all performed (Example 1).

[Experimental Example 4]

100 adult men and women of 20 to 50 years of age were allowed to use the skin whitening cosmetics of Example 5 and Comparative Examples 8 to 10 once a day for 1 month, and the skin condition before and after use was surveyed and the results were obtained. The average of was summarized in the form of Table 3 below.

[Table 3]

In Table 3, 1 point is very poor, 2 points are poor, 3 points are normal, 4 points are excellent, and 5 points are excellent.

Referring to Table 3, using only the green barley extract (Comparative Example 8) or using only the various extracts and niacin amide described above (Comparative Example 9), using other natural extracts together (Example 5) ) Turned out to be more desirable. In addition, referring to Tables 2 and 3, it can be seen that the skin whitening performance varies according to the method of forming the green barley extract.

The above description of the present application is for illustrative purposes, and those skilled in the art to which the present application pertains will understand that it is possible to easily modify to other specific forms without changing the technical spirit or essential features of the present application. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present application is indicated by the claims below, rather than the detailed description, and it should be interpreted that all modifications or variations derived from the meaning and scope of the claims and equivalent concepts thereof are included in the scope of the present application.

Claims (9)

Comprising the step of fermenting green barley by Lactobacillus acidophilus lactic acid bacteria, photo-processing and crushing, mixing the crushed green barley powder with solvent and ethanol to extract reflux, and fractionating the extract The step of forming a green barley extract;
Forming a skin lightening agent comprising the green barley extract; And
Mixing the skin whitening agent, moisturizing agent, viscosity increasing agent, and surfactant to form a skin whitening cosmetic;
Including,
The wavelength of light used in the step of photo-processing the green barley is 200 nm to 900 nm,
The wavelength of light is changed at a rate of 1 nm/min to 20 nm/min,
The extraction step includes an elevated temperature condition, agitation conditions, and ultrasonic irradiation conditions,
The extraction step is performed at a temperature increase of 1 ℃ / min to 20 ℃ / min,
The stirring is performed at a speed of 10 rpm to 300 rpm,
The ultrasound has a frequency of 10 kHz to 1 MHz,
The skin whitening cosmetic tyrosinase (tyrosinase) secretion inhibition rate and melanin synthesis inhibition rate is increased depending on the concentration of the green barley extract,
Method of manufacturing skin whitening cosmetics.
delete delete According to claim 1,
The tyrosinase (tyrosinase) secretion inhibition rate is 40% to 90%, the melanin synthesis inhibition rate is 35% to 90%, the method for producing skin whitening cosmetics.
According to claim 1,
The skin lightening agent is buckwheat extract, lemon balm extract, natural extract, niacinamide, arbutin, ethyl ascorbic acid, ascorbyl tetraisopalmitate, and combinations thereof The method comprising the one further selected from the group consisting of, skin whitening cosmetics.
The method of claim 5,
The natural extracts include bee odor extract, mulberry extract, green light callus extract, bamboo callus extract, rice cake butt extract, snow juniper extract, imperial flower extract, red sea ginseng extract, sea cucumber extract, civil incense extract, freshwater laver extract, and combinations thereof The method comprising the one selected from the group consisting of skin whitening cosmetics.
According to claim 1,
The concentration of the green barley extract is 10 μg/ml to 400 μg/ml, a method for producing skin whitening cosmetics.
According to claim 1,
The solvent is hexane, chloroform, ethanol, water, and those selected from the group consisting of combinations thereof, the method for producing skin whitening cosmetics.
delete

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