KR20220018191A - A cosmetic composition for promoting growth of eyelash comprising fermentation product of jujube seed - Google Patents

Abstract

The present invention relates to a cosmetic composition for promoting the eyelash growth comprising a fermented jujube seed product by means of Phellinus linteus. More specifically, the present invention relates to a cosmetic composition for promoting the eyelash growth comprising a fermented product obtained by inoculating jujube seed powder with a culture solution of Phellinus linteus and culturing the same. The fermented jujube seed product fermented by means of Phellinus linteus of the present invention is a composition derived from natural products without cytotoxicity and thus has excellent eyelash growth promoting activity. Accordingly, the fermented jujube seed product can be applicable as a raw material composition of a functional cosmetic composition and thus can be very useful in the cosmetic industry.

Description

COSMETIC COMPOSITION FOR PROMOTING GROWTH OF EYELASH COMPRISING FERMENTATION PRODUCT OF JUJUBE SEED}

The present invention is situation mushroom mycelium ( Phellinus baumii Mycelium) relates to a cosmetic composition for promoting eyelash growth comprising a fermented product of jujube seeds, and more particularly, a fermented product obtained by inoculating and culturing a jujube seed powder in a Mycelium culture medium of Sanghwang mushroom. It relates to a cosmetic composition for promoting eyelash growth, comprising.

As interest in beauty increases in modern society, the desire for the functions of cosmetics is increasing, and high-functional products are continuously appearing accordingly.

In general, makeup is done much more by women than by men, and various types of cosmetics have been developed and used depending on the area where makeup is applied. In particular, women pay a lot of attention to eye makeup because the eyes occupy a very large part in judging a woman's beauty, and eye makeup is the most convenient and effective part of the face to change the impression. When it comes to eye makeup, the thicker and richer the eyebrows and eyelashes are, the clearer the eyes are to make the face stand out.

In particular, eyelashes are one of the facial parts that can emphasize femininity the most, and as the eyelashes are thick enough to stand out, the longer the eyelashes, the bigger and clearer the eyes can be seen.

These eyelashes show a significant difference from the hair of other parts, such as the hair of the scalp, in terms of the cycle of the growth and resting phases. In the case of hair, the growth phase lasts for about 3 to 4 years, the telogen lasts for 3 to 4 months, the growth phase accounts for 85-93% of the total cycle, and the telogen ratio accounts for 7-15% of the total cycle. The growth rate is about 0.4 mm/day. On the other hand, in the case of eyelashes, the growth phase lasts 6 to 10 weeks, and the resting period is maintained for 4 to 9 months, the growth phase accounts for 10-20% of the total cycle, and the resting phase accounts for 80-90% of the total cycle. The growth rate of eyelashes is about 80-280 μm/day, which is quite different from the growth rate of hair. Despite these differences, conventionally, a hair thickening agent (hair-growth agent) used for hair has been used to promote eyelash growth, but due to the difference between the hair and the eyelashes, the same effect as in the hair does not occur in the eyelashes. Furthermore, eyelashes are located in the vicinity of the weakest of the human body's membranes, so there has been a problem of causing a serious risk to the eyes when using a conventional hair thickening agent (hair growth agent).

On the other hand, cosmetics for eyelashes include eye shadow, eye line, eye pencil, mascara, and the like. Among them, mascara is a cosmetic for eyelashes that most women use essential for makeup. Its purpose is to darken and straighten the eyelashes to make the eyelashes appear clearer and longer to make the eyes look more beautiful.

However, even if mascara is applied to the eyelashes, the eyelashes themselves do not become longer. Therefore, if the eyelashes are used by women with short or thin eyelashes, the effect is not great, so artificial eyelashes are used to improve this problem. can be However, artificial eyelashes have the disadvantage that it is not easy for the user to directly attach the artificial eyelashes to the eyes, requiring a professional's hand. There is a problem that there are many cases where it is visible.

In the cosmetic field, research is being conducted to develop an eyelash nutrient or eyelash thickener that can grow or promote the growth of eyelashes rather than simply making eyelashes look longer. Therefore, it is essential to ensure human body stability.

For example, Korean Patent Registration No. 10-1270965 discloses a composition comprising natural products such as paprika, chrysanthemum, goji berries, mulberry leaves, pine needles, seaweed and black hemp extract as active ingredients as a cosmetic composition for promoting eyebrow or eyelash growth. In the Republic of Korea Patent No. 10-12073175, Geumju green, a mixture selected from the group consisting of extracts of Manhyeongja, Guritdae, amethyst, chrysanthemum, fenugreek, Bokbunja, Saengjihwang, Youngneunghyang, Mangcho, Clove, Gwonbaek, Chookchokhwa, and Gajabi. A cosmetic composition for promoting growth of eyebrows and eyelashes has been described containing a herbal extract of Unyu, and in Korean Patent Publication No. 10-2005-0077310, an eyelash growth promoter comprising an extract of polygonum tinctorium belonging to the Polygonaceae family. has been disclosed.

On the other hand, in the case of a natural extract through fermentation, the sugar component present in the natural product is used as a carbon source in the metabolic process of microorganisms without using an organic solvent harmful to the human body and converted into alcohol. Polyphenols, catechins, etc. are saccharified to exist as stable compounds, so the extraction rate of the active ingredient content is increased through fermentation, and excellent efficacy is expected by the active ingredient activated by excluding sugar.

Recently, as consumers' awareness of mushrooms has been expanded to not only edible but also medicinal and favorite foods, and mushroom fruiting bodies and extracts of mycelium or mycelium cultures have been found to be effective in the prevention and treatment of various diseases, mushrooms are a new bio-industrial material. It has emerged as an important biological resource for the development of functional foods and pharmaceuticals. About 20,000 species of mushrooms are known worldwide, of which about 2,000 can be developed for food. There are about 992 types of mushrooms distributed in Korea, of which 100 are edible and 50 are poisonous, and 20 are particularly toxic. More than 20 kinds of mushrooms can be cultivated in Korea and have been proven to have anti-cancer properties, lowering cholesterol, and lowering blood sugar.

As a representative example, the Sanghwang mushroom is also called a woody mud mushroom, and the cap has various shapes such as a diameter of 6 to 12 cm, a thickness of 2 to 10 cm, a semicircle shape, a flat shape, a round mountain shape, and a horseshoe shape. The dark brown hairs are short and dense on the surface, disappear as they grow and become keratinized. In the early stages of growth, it looks like a lump of mud, but when it is fully grown, its tongue sticks out of a tree stump, so it is also called Suseol (樹舌). Many studies have been conducted since it was known to have anticancer effects by Kiekawa et al. in Japan in 1968. In addition to the anticancer effect, it exhibits various pharmacological effects such as immunomodulatory effect, antioxidant effect, liver function improvement effect, hyperlipidemia improvement effect, and whitening effect. For medicinal purposes, it is yellow or pale yellow when it is moonshine, and it is characterized by its lack of taste and fragrance. It is native to Korea, Japan, Australia, and North America.

On the other hand, jujube contains about 28% of its main components, glucose and fructose, and about 1.5% of protein, and other calcium, iron, phosphorus, vitamin A and vitamin B. Jujube extract has been reported to have good liver recovery and protective effects. In addition, jujube is used as a relieving tonic in oriental medicine, and it is known that jujube has various pharmacological effects such as antipyretic, relieving gastrointestinal disease, relieving insomnia, and relieving systemic weakness. It is known that the ingredients that implement the efficacy of these jujubes are especially contained in jujube seeds. In addition, it is known that jujube seeds contain a significant amount of active ingredients such as flavonoids and polyphenols compared to other types of seeds.

However, the reality is that jujube seeds are not used for food as well as other purposes because their tissues are dense and hard, and most of them are thrown away. Research on jujube seeds is very scarce.

KR 10-2012-0039384 A KR 10-2019-0012602 A KR 10-2019-0001854 A

The present invention has been devised in order to solve the problems of the prior art as described above, and the problem to be solved in the present invention is to promote eyelash growth, including a fermented product obtained by fermenting jujube seeds with Sanghwang mushroom mycelium as a natural product without cytotoxicity. An object of the present invention is to provide a cosmetic composition.

The present invention has been devised to solve the above problems, and the present invention provides a cosmetic composition for promoting eyelash growth comprising a fermented product of jujube seed (seed of Ziziphus jujube ) by inoculation with the mycelium of the Sangha mushroom.

The fermented jujube seed by inoculation of the Sangha mushroom mycelium includes the steps of (1) drying and pulverizing jujube seeds; (2) inoculating the mycelium culture medium of Sanghwang mushroom into a culture flask containing powdered jujube seeds; (3) culturing under the conditions of a culture temperature of 20 to 35° C., a relative humidity of 70 to 90%, and a culture period of 7 to 45 days; and (4) hot water extraction and pulverization.

It is preferable that the cosmetic composition for promoting the growth of the eyelashes further comprises a fermented jujube seed by inoculation of the Cordyceps mycelium.

The fermented jujube seed by the Cordyceps mycelium inoculation step (1) drying and pulverizing the jujube seeds; (2) inoculating a culture solution of Cordyceps mycelium in a culture flask containing powdered jujube seeds; (3) culturing under the conditions of a culture temperature of 20 to 35° C., a relative humidity of 70 to 90%, and a culture period of 7 to 45 days; and (4) hot water extraction and pulverization.

The cosmetic composition is a formulation selected from the group consisting of mascara, eyeliner, eye shadow, eyebrow, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, nourishing lotion, nourishing cream, essence, nourishing essence and pack. It is preferable to be

The fermented jujube seed product fermented with the Sanghwang mushroom mycelium of the present invention is a composition derived from a natural product without cytotoxicity, and has excellent eyelash growth promoting activity.

1 is a flowchart showing a manufacturing process of the cosmetic composition for promoting eyelash growth of the present invention.

Hereinafter, the present invention will be described in detail.

As a result of repeated research to develop a material for a functional cosmetic composition using a natural product, the inventors of the present invention inoculate the jujube seed powder with the Sangha mushroom mycelium, and culture it according to a solid culture method. It is confirmed that the fermented product has an excellent eyelash growth promoting effect, and it is suggested that the fermented jujube seed by the Sangha mushroom mycelium of the present invention can be used as a raw material composition for a functional cosmetic composition.

Accordingly, the present invention provides a cosmetic composition for promoting eyelash growth comprising a fermented product of jujube seeds by inoculation of the mycelium of Sangha mushroom.

As a preferred embodiment, the fermented jujube seed by inoculation of the mycelium of the Sangha mushroom of the present invention comprises the steps of (1) drying and pulverizing jujube seeds; (2) inoculating the mycelium culture medium of Sanghwang mushroom into a culture flask containing powdered jujube seeds; (3) culturing under the conditions of a culture temperature of 20 to 35° C., a relative humidity of 70 to 90%, and a culture period of 7 to 45 days; and (4) hot water extraction and pulverization.

Jujube seeds of the present invention refer to seeds of jujube, and after washing the prepared jujube seeds with water, dehydrating and drying them, pulverizing them, and then sterilizing them by various known sterilization methods. In a preferred embodiment, the powdering has a diameter of 0.1 to 5 mm, and sterilization may be performed by autoclaving at 121° C. for 30 minutes.

The Sanghwang mushroom mycelium culture medium of the present invention can be prepared by culturing the Sanghwang mushroom spawn. The Sanghwang mushroom starter can be periodically subcultured and maintained until use. As a preferred embodiment, after inoculating the Sanghwang mushroom mycelium in PDA (Potato Dextrose Agar) medium, passage at a temperature of 20 to 25° C. at intervals of 3 to 4 weeks. It can be cultivated and maintained. The seed culture is a process of culturing the Sanghwang mushroom mycelium spawn prior to the main culture. In a preferred embodiment, the seed culture can be obtained as a liquid culture by inoculating a seed, ie, mycelium, in a medium for the mycelium of the Sangha mushroom mycelium, and then culturing it with rotation shaking.

The method of inoculating the Sanghwang mushroom mycelium of the present invention into the sterilized jujube seed powder is aseptically inoculating a certain amount of the liquid culture obtained by the above-described Sanghwang mushroom seed culture into the sterilized jujube seed powder. For example, 30 to 70 parts by weight of the Sangha mushroom mycelium culture solution can be inoculated with respect to 100 parts by weight of jujube seed powder in a flask container with a volume of 5 to 10 times.

After inoculation of the Sangha mushroom mycelium, a culture process is performed, and the culture conditions are preferably a temperature of 20 to 35 ° C, a relative humidity of 70 to 90%, and a culture period of 7 to 45 days, and in a preferred embodiment, at 24 ° C. It can be cultured for 20 days in an incubator of 85% relative humidity at temperature.

The fermented jujube seed of Sanghwang mushroom mycelium, which has been fermented, has a shape in which the Sanghwang mushroom mycelium is entirely covered on the surface of the jujube seed powder. .

The solvent used for extraction may be water or lower ethanol, but is preferably hot water extraction. In a preferred embodiment, about 5-150 times the volume of hot water is added to the fermented jujube seed of the dried and pulverized Sanghwang mushroom mycelium, shaken for 1-5 hours, and filtered or centrifuged to obtain the extract. The above process may be performed 1 to 5 times, and preferably, the extraction may be repeated 3 times. The obtained extract is sterilized in the same manner as in the sterilization process described above.

It is preferable that the cosmetic composition for promoting eyelash growth of the present invention further comprises a fermented jujube seed by inoculation of a Cordyceps mycelium in addition to the fermented jujube seed product fermented with the above-mentioned Sanghwang mushroom mycelium.

As a preferred embodiment, the fermented jujube seed by the Cordyceps mycelium inoculation comprises the steps of (1) drying and pulverizing jujube seeds; (2) inoculating a culture solution of Cordyceps mycelium in a culture flask containing powdered jujube seeds; (3) culturing under the conditions of a culture temperature of 20 to 35° C., a relative humidity of 70 to 90%, and a culture period of 7 to 45 days; and (4) hot water extraction and pulverization.

Cordyceps of the present invention is a fungus belonging to the Ascomycetes ergotomycetes Cordyceps, specifically, Cordyceps spp., Paecilomyces spp., Isaria spp., Phytocody It is classified into about 100 genera including Phytocordyceps spp., and about 750 species are known worldwide.

In the present invention, all of these cordyceps can be used, and preferably, Cordyceps sinensis , Cordyceps militaris , Cordyceps scarabaecola , Cordyceps nutans ( Cordyceps nutans ), Cordyceps sphecocephala , Cordyceps tricentri , Paecilomyceps tenuipes and Paecilomyces japonica selected from the group consisting of , More preferably, Paecilomyces japonica also named Snow Cordyceps ( Paecilomyces japonica ).

The Cordyceps mycelium culture solution of the present invention can be prepared by culturing the Cordyceps seed strain. The cordyceps seed can be periodically subcultured and maintained until use. As a preferred embodiment, after inoculating the Cordyceps mycelium in PDA (Potato Dextrose Agar) medium, subculture at 20-25 ° C. at intervals of 3 to 4 weeks. can keep The seed culture is a process of culturing the stored Cordyceps mycelium spawn prior to the main culture. As a preferred embodiment, the seed culture can be obtained as a liquid culture by inoculating a seed, that is, mycelium in a medium for cordyceps mycelium, and then culturing it with rotation shaking.

The method of inoculating the Cordyceps mycelium of the present invention into the sterilized jujube seed powder is aseptically inoculating a certain amount of the liquid culture obtained by the above-described Cordyceps seed culture into the sterilized jujube seed powder. For example, 30 to 70 parts by weight of the Cordyceps mycelium culture solution may be inoculated with respect to 100 parts by weight of jujube seed powder in a flask container with a volume of 5 to 10 times.

After inoculation of the Cordyceps mycelium, a culture process is performed, and the culture conditions are preferably a temperature of 20 to 35 ° C, a relative humidity of 70 to 90%, and a culture period of 7 to 45 days, and in a preferred embodiment, a temperature of 24 ° C. It can be cultured for 20 days in an incubator at 85% relative humidity.

The fermented fermented jujube seed of the Cordyceps mycelium is a shape in which the Cordyceps mycelium is entirely covered on the surface of the date seed powder, and after drying and pulverizing it, an extract of the fermented jujube seed of the Cordyceps mycelium can be prepared.

The solvent used for extraction may be water or lower ethanol, but is preferably hot water extraction. As a preferred embodiment, about 5-150 times the volume of hot water is added to the fermented jujube seed of the dried and pulverized Cordyceps mycelium, shaken for 1-5 hours, and filtered or centrifuged to obtain the extract. The above process may be performed 1 to 5 times, and preferably, the extraction may be repeated 3 times. The obtained extract is sterilized in the same manner as in the sterilization process described above.

The cosmetic composition for promoting eyelash growth includes mascara, eyeliner, eye shadow, eyebrow, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, nourishing lotion, nourishing cream, essence, nourishing essence and pack. It is preferred that the formulation is selected from

As a preferred embodiment, the cosmetic composition for promoting eyelash growth of the present invention may be prepared to contain 0.001 to 50% by weight of the fermented jujube seed by inoculation of the mycelium of the Sangha mushroom.

As another preferred embodiment, the cosmetic composition for promoting eyelash growth of the present invention may be prepared to contain 0.001 to 50% by weight of a mixture of a fermented jujube seed product by inoculation with Sangha mushroom mycelium and a fermented jujube seed product by inoculation with Cordyceps mycelium. , the mixing ratio of the mixture may be in the range of 1:99 to 99:1.

In this content range, proper formulation stability can be ensured, and the desired effect of promoting eyelash growth can be expected.

In addition to the fermented jujube seed, the cosmetic composition of the present invention can be used by additionally including a known natural extract or other ingredients for the effects of antioxidant, skin aging prevention and skin regeneration promotion within the range that does not inhibit the effective activity of the present invention. have.

In addition, the composition may contain ingredients commonly added to cosmetic compositions, for example, fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, Cosmetics, such as fragrances, surfactants, water, ionic or non-ionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives; It may be molded into a specific formulation including an adjuvant or carrier commonly used in the field of dermatology.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, pack, soap, surfactant- It may be formulated as containing cleansing, oil and spray, but is not limited thereto.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propellants such as propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used.

Hereinafter, the present invention will be described in more detail through preferred embodiments. The following examples describe a preferred embodiment of the present invention, and the scope of the present invention is not limited and interpreted by the following examples.

[Example]

1. Jujube seeding by Sanghwang mushroom mycelium filial piety hot water extract preparation

Longevity mushroom ( Phellinus ) baumii ) was used after receiving a strain from Farm Bios Co., Ltd. for passage. It was cultured using PDA (Potato Dextrose Agar, Difco, Co., USA) medium at 25°C, subcultured at 4 week intervals, and stored at 4°C. After inoculation of the mycelium of the longevity sphagnum mycelium under passage into a 100ml volume of mushroom mycelium solid medium (1 cm × 1 cm, 3 pieces) in a 500ml flask, in a rotary shaker incubator (Daihan Labtech Co.Korea, 25±1℃, 130rpm) It was cultured for 7 days and used as a liquid seed culture.

Jujubes were purchased from the Daegu herbal medicine market, the jujube seeds were selected, washed with water before use to remove impurities, dehydrated, and then powdered. 100 g of jujube seed powder was placed in a 3L culture vessel, and after high-pressure sterilization at 121° C. for 30 minutes, it was cooled at room temperature, and 60 ml of the prepared liquid seed was inoculated aseptically into 100 g of sterilized jujube seed powder. Fermentation was carried out for 20 days in an incubator at 24° C. and 85% relative humidity. After fermentation, 10 times the volume of hot water was added, shaken for 2 hours, immersed for 72 hours, centrifuged at 10,000 rpm for 10 minutes, and the supernatant was taken. After repeating this 3 times, the extract was concentrated under reduced pressure to prepare a powder was used.

2. Preparation of hot water extract of fermented jujube seeds by Cordyceps mycelium

Snow Cordyceps Cordyceps ( Paecilomyces japonica ) was used after receiving the strain under passage from Farm Bios Co., Ltd. It was cultured using PDA (Potato Dextrose Agar, Difco, Co., USA) medium at 25°C, subcultured at 4 week intervals, and stored at 4°C. After inoculating the sub-cultured Cordyceps Cordyceps mycelium in a 100ml volume of mushroom mycelium solid medium (1 cm × 1 cm, 3 pcs.) in a 500ml flask, 7 in a rotary shaking incubator (Daihan Labtech Co.Korea, 25±1℃, 130rpm) It was cultured for one day and used as a liquid seed culture.

Jujubes were purchased from the Daegu herbal medicine market, the jujube seeds were selected, washed with water before use to remove impurities, dehydrated, and then powdered. 100 g of jujube seed powder was placed in a 3L culture vessel, and after high-pressure sterilization at 121° C. for 30 minutes, it was cooled at room temperature, and 60 ml of the prepared liquid seed was inoculated aseptically into 100 g of sterilized jujube seed powder. Fermentation was carried out for 20 days in an incubator at 24° C. and 85% relative humidity. After fermentation, 10 times the volume of hot water was added, shaken for 2 hours, immersed for 72 hours, centrifuged at 10,000 rpm for 10 minutes, and the supernatant was taken. After repeating this 3 times, the extract was concentrated under reduced pressure to prepare a powder was used.

3. Preparation of test samples

The extract powder of the fermented jujube seed prepared in items 1 and 2 above was dissolved in lotion to a final concentration of 20% by weight at the content shown in Table 1 below, and prepared as samples of Examples 1 to 5, respectively.

division situation mushroom
Jujube seed fermented product (wt%) cordyceps
Jujube seed fermented product (wt%) Total (wt%) Example 1 20 0 100 Example 2 15 5 100 Example 3 10 10 100 Example 4 5 15 100 Example 5 0 20 100 comparative example 0 0 100

4. Test the effect of promoting eyelash growth

In order to compare the eyelash growth promoting effect of the samples obtained in Examples 1 to 5 and Comparative Examples, the preparations in Examples 1 to 5 and Comparative Examples for each test group (n = 5) targeting 30 healthy adult women The eyelash growth effect was measured by applying a sample for promoting eyelash growth before and after washing twice a day for 3 weeks, respectively. The average value of the length of eyelashes grown in each test group (n = 5) was arithmetic and shown in Table 2 below.

division Eyelash growth length (mm) Example 1 0.4 Example 2 1.3 Example 3 0.8 Example 4 0.3 Example 5 0.2 comparative example 0

As shown in Table 2, it was confirmed that the fermented jujube seed of the present invention had an effect of promoting growth of eyelash length without any side effects. In particular, the fermented jujube seed obtained by inoculation of the Sanghang mushroom mycelium had better eyelash growth promoting activity than the fermented jujube seed obtained by inoculation with the mycelium of Cordyceps mycelium. When the fermented jujube seed by mycelium inoculation was mixed in a weight ratio of 50:50 to 75:25, it was confirmed that the most excellent eyelash growth promoting effect was obtained.

Claims (5)

A cosmetic composition for promoting eyelash growth, comprising a fermented product of jujube seeds (seed of Ziziphus jujube ) by mycelium inoculation of Sanghwang mushroom. [Claim 2] The cosmetic composition for promoting eyelash growth according to claim 1, wherein the fermented jujube seed by inoculation of the mycelium of the Sangha mushroom mycelium is obtained by comprising the following steps:
(1) drying and pulverizing jujube seeds;
(2) inoculating the mycelium culture medium of Sanghwang mushroom into a culture flask containing powdered jujube seeds;
(3) culturing under the conditions of a culture temperature of 20 to 35° C., a relative humidity of 70 to 90%, and a culture period of 7 to 45 days; and
(4) hot water extraction and pulverization. [Claim 2] The cosmetic composition for promoting eyelash growth according to claim 1, further comprising a fermented jujube seed product by inoculation of the Cordyceps mycelium. The cosmetic composition for promoting eyelash growth according to claim 3, wherein the fermented jujube seed by inoculation of the Cordyceps mycelium is obtained by comprising the following steps:
(1) drying and pulverizing jujube seeds;
(2) inoculating a culture solution of Cordyceps mycelium in a culture flask containing powdered jujube seeds;
(3) culturing under the conditions of a culture temperature of 20 to 35° C., a relative humidity of 70 to 90%, and a culture period of 7 to 45 days; and
(4) hot water extraction and pulverization. According to any one of claims 1 to 4, wherein the cosmetic composition is mascara, eyeliner, eye shadow, eyebrow, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, nourishing lotion, nourishing cream A cosmetic composition for promoting eyelash growth, characterized in that the formulation is selected from the group consisting of , essence, nutritional essence and pack.

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