KR102051939B1 - Manufacturing method and cosmetic ingredient using Dendropanax morbifera Lev extraction fluid - Google Patents

Abstract

The present invention relates to a method for producing a cosmetic composition using a dendropanax morbifera extract fermentation product. The method for producing a cosmetic composition using a dendropanax morbifera extract fermentation product comprises: a first step of washing dendropanax morbifera with water and then, dipping and washing the same in distilled water of 45-50°C for an hour; a second step of removing distilled water by drying the dendropanax morbifera completed with a washing, pulverizing the dendropanax morbifera to be 200 mesh or less by using a mixer, and then performing ultrasonic treatment for 5-7 minutes by using an ultrasonic cleaner on 150 parts by weight of ethanol based on 100 parts by weight of the dendropanax morbifera; a third step of heating and drying dendropanax morbifera at 80°C to remove the ethanol after completing the ultrasonic treatment, and producing dendropanax morbifera extract from the dried dendropanax morbifera by using supercritical extraction; a fourth step of producing a dendropanax morbifera extract fermentation product by fermenting the dendropanax morbifera extract; a fifth step of adding a first additive made of a natural substance to the dendropanax morbifera extract fermentation product; a sixth step of, after finishing the addition of the first additive, stirring the same at 80-100 rpm at the temperature of 35-40°C while primarily aging for 6-8 hours to produce a first mixture; and a seventh step of producing a second mixture by adding herb extract to the first mixture. The present invention is able to enhance a whitening effect on skin and inhibit melanin production.

Description

Manufacturing method and cosmetic composition produced by the Hwangchil wood extract fermentation {Manufacturing method and cosmetic ingredient using Dendropanax morbifera Lev extraction fluid}

The present invention relates to a method for producing a cosmetic composition comprising the extract of the Hwangchil wood, and to a cosmetic composition produced by the same, and more particularly, to extract the Hwangchil wood in a novel manner, and the extract thus obtained is functional through fermentation Maximizing, and relates to a method for producing a cosmetic composition comprising the extract of the Hwangchil wood comprising a natural substance that can further improve the function of the Hwangchil wood and the cosmetic composition produced thereby.

Skin exposed to ultraviolet rays protects skin by tyrosine in melanocytes (tyrosine) oxidizes by tyrosinase biosynthetic action to produce melanin, increasing skin's resistance to ultraviolet rays, dryness, and extreme temperatures. Excessive melanin production leads to pigmentation, resulting in hyperpigmented diseases such as blemishes, freckles, blotch, spots, otamoban, milk spots, post-inflammatory pigmentation and clumping (Chenm, 1991; Seong Ki-cheon and Kim Jun-soo, 2005).

Blemishes are common in females of childbearing age and are pigmented disorders with brown irregular borders that show brown spots of various sizes and irregular shapes on the UV-exposed area. Freckles are also common in Caucasians and are often caused by genetic factors, and irregular, yellow, black, and brown pigment spots appear on environmental and UV-exposed areas (Reenstra et al., 1993). Black mushrooms are caused by weakening of melanin's defense mechanisms and a decrease in metabolic functions (Miyamura et al., 2006; Sulaimon, 2003).

The development of effective whitening agents for skin whitening is largely made in three directions: inhibiting melanin production in melanocytes, controlling melanocyte stimulants, and promoting melanin excretion. Melanocytes in the skin connect with adjacent keratinocytes through dendritic dendritic cells to form epidermal melanin units, which play an important role in the transport of melanosomes. Melanin is synthesized by the continuous oxidation of enzymes in melanocytes within the epidermal basal melanocytes. The starting material of melanin synthesis is tyrosine, a type of amino acid, tyrosine is oxidized by tyrosinase to L-3,4-dihydroxyl-L-phenylalanine (L-DOPA), which is oxidized to dopa quinone and DOPA quinone to DOPAchrome, 5,6-dihydroxyin-dole, indole 5,6-quinone to finally synthesize melanin (Prota, 1992; Lopez et al., 1992). The tyrosinase inhibitors known to date include resorcinol, hydroquinone, 4-hydroxyanisole, ascorbic acid and its derivatives, kojic acid, arbutin, glucosamine, oxyreseveratrol, viniferin, ferulic acid, etc. Only a limited amount of is used as a whitening additive (Ando et al., 1993; Imokawa et al., 1982). Therefore, the development or research of functional products derived from natural products is continuously underway (Lim Do-yeon, 2012).

There is a need for research on new materials that have whitening functionality but no problems such as side effects or toxicity. In this regard, researches are being actively conducted in the pharmaceutical and cosmetic industries to search for and isolate substances that effectively inhibit the biosynthesis of melanin using medicinal plants used in herbal or folk medicine to prevent pigmentation. (Jin Jong-eon, 2002). On the basis of these previous studies, studies on the verification of skin whitening activity using natural plants as a raw material source of cosmetics or quasi-drug products have been actively conducted.

It is an object of the present invention to improve the effect on skin whitening by using the extract and the natural fermentation of Hwangchil wood, a method for producing a cosmetic composition comprising the Hwangchil wood extract fermentation that can inhibit the production of melanin and produced by To provide a cosmetic composition.

In addition, another object of the present invention is a method for producing a cosmetic composition comprising a yellow lacquer extract fermentation that can streamline the production process to produce a uniform performance without producing a difference in batch sugar for the production of the yellow lacquer extract and It is to provide a cosmetic composition prepared thereby.

In addition, another object of the present invention can increase the shelf life of the extract and fermentation of the Hwangchil wood by further comprising a herbal material, it can omit a separate chemical fragrance additives by the herbal aroma generated by itself It is to provide a method for producing a cosmetic composition comprising the extract of the Hwangchil wood and a cosmetic composition produced thereby.

According to one aspect of the present invention, embodiments of the present invention after washing the yellow chilchi using water, the first step of washing by dipping for 1 hour in distilled water of 45 ℃ to 50 ℃; After the washing is completed to remove the distilled water by drying the yellow lacquer tree, using a mixer to grind the yellow lacquer tree to 200 mesh or less, 150 parts by weight of ethanol based on 100 parts by weight of the yellow lacquer tree using an ultrasonic cleaner for 5 minutes to A second step of performing sonication for 7 minutes; After the sonication is completed by heating to 80 ℃ to remove the ethanol and dried, the third step of producing the extract from the yellow lacquer wood by supercritical extraction; A fourth step of preparing the fermented hwangchil wood extract by fermenting the hwangchil wood extract; A fifth step of adding a first additive made of a natural substance to the extract of the hwangchil wood; A sixth step of preparing the first mixture by first aging for 6 to 8 hours with stirring at 80 rpm to 100 rpm at a temperature of 35 ° C. to 40 ° C. after the addition of the first additive; And a seventh step of preparing a second mixture by adding a herb extract to the first mixture.

In the third step, the supercritical extraction is performed in a supercritical extraction tank having a pressure of 400 bar and a temperature of 50 ° C., and a separation tank having a pressure of 40 bar and a temperature of 40 ° C., and a flow rate of ethanol as a cosolvent is 5 per minute. It can be carried out for 2 hours by adding to ml.

In the fourth step, the yellow lacquer extract fermentation, 600 parts by weight of distilled water is added to 100 parts by weight of the yellow lacquer extract extract, and 5 parts by weight of the lactobacillus culture solution to 100 parts by weight of the yellow lacquer extract extract. The fermentation broth was prepared by fermentation at 36 ° C. for 2 weeks, and the lactic acid bacteria culture solution was added with 4 g of co-enzyme alpha-herbzyme to 50 g of the yellow lacquer powder and contacted with lactic acid bacteria at 37 ° C. It can be obtained by incubating for 3 days at.

The lactic acid bacteria, Lactobacillus sakei (Lactobacillus sakei B2-16), Lactobacillus brevis (Lactobacillus brevis), Lactobacillus plantarum P23, Lactococcus lactis (Lactococcus lactis A164) and Bacillus coagulus (Bacillus coagulance) may be any one or more selected from the group consisting of.

In the fifth step, the first additive comprises Centella asiatica extract and Angelica Root Extract, wherein the first additive is to prepare the Centella asiatica extract, to prepare the Angelica nigrai extract, the Centella asiatica and Angelica The root extract may be mixed to produce nanoparticles.

Preparation of the centella extract, after washing the centella is hot-air dried at 40 ℃ and powdered to a diameter of 10 ㎛ to 0.5mm by using a blender, ethanol 1000 based on 100 parts by weight of the centellae in the powdered centella Ultrasonic extraction for 30 minutes by weight, centrifugation at 3000rpm for 10 minutes after the ultrasonic extraction is completed, the supernatant was filtered with a 0.45㎛ syringe filter and concentrated with a reduced pressure concentrator, and concentrated centrifugal extract After storage for 24 hours in a freezer at -70 ℃ can be powdered with a freeze dryer.

The extract of the Angelica Root Extract, after washing the Angelica Root in water and dried for 10 days in a cool place, and the 1000 mg of ethanol on the basis of 100 parts by weight of the Angelica Root in 100 rpm After stirring three times a day in a shaking incubator at 25 ° C. with stirring, the supernatant and sediment were filtered and concentrated by a vacuum condenser. After storage for 24 hours in the powder may be lyophilized.

To prepare the nanoparticles by mixing the Centella asiatica extract and Angelica Root Extract, adding lecithin and cholesterol in a molar ratio into the reactor, chloroform and methanol, and stirring at 100 rpm to 200 rpm for 3 hours to dissolve lecithin and cholesterol, The chloroform and methanol were dried at 40 ° C. for 4 hours until the chloroform and methanol were completely removed, thereby preparing a lipid layer, and the powdered Centella asiatica and Chalcatas chinensis extract were dissolved in distilled water at a ratio of 1: 1. After preparing at a concentration of 10mg / mL, the lipid membrane is put into a prepared reactor can be prepared as nanoparticles by homogenizing for 1 hour at room temperature using an ultrasonic mill.

In the seventh step, the herbal extract contains tea tree oil, the herbal extract is mixed with 25ml to 50ml of the tea tree oil with respect to 1000ml of distilled water, stirred at 250rpm for 30 minutes at room temperature, and stirred After the completion of the high pressure emulsification process at 3 cycles at 1,000 bar in order to reduce and homogenize the particle size of the tea tree oil mixed in the distilled water, the frequency of 65kHz to 75kHz at a temperature of 80 ℃ Ultrasound having can be applied for 2 hours to 3 hours.

According to another aspect of the present invention, it comprises a cosmetic composition using the extract of the Hwangchil wood produced by the method described above.

The cosmetic composition may further include one or more additives selected from the group consisting of antioxidants, stabilizers, solubilizers, vitamins, pigments, flavors and carriers.

The cosmetic composition may be prepared in one or more formulations selected from lotion, nutrition cream, eye cream, moisture cream, lotion, pack, essence.

According to the present invention as described above, the method for producing a cosmetic composition comprising an extract fermentation Hwangchil wood to improve the effect on skin whitening and inhibit the production of melanin by using the extract from the Hwangchil wood and natural substances and Thereby it can provide a cosmetic composition prepared.

In addition, according to the present invention and a method for producing a cosmetic composition comprising the extract from the yellow lacquer wood that can streamline the production process so as to produce a uniform performance without producing a difference in batch sugar during the production It can provide a prepared cosmetic composition.

In addition, according to the present invention by further including the herbal material can be increased storage period of the extract and fermentation of the Hwangchil wood, Hwangchil wood extract that can omit a separate chemical fragrance additive by the herb generated by itself It can provide a method for producing a cosmetic composition comprising a fermented product and a cosmetic composition produced thereby.

1 is a flow chart showing a method for manufacturing a cosmetic composition using the extract of the Hwangchil wood according to an embodiment of the present invention.
2 is a graph showing DPPH Free Radical scavenging activity according to Examples and Comparative Examples of the present invention.

Specific details of other embodiments are included in the detailed description and the drawings.

Advantages and features of the present invention, and methods for achieving them will become apparent with reference to the embodiments described below in detail in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various forms. In the following description, when a part is connected to another part, it is only directly connected. It also includes cases where other media are connected in between. In the drawings, parts irrelevant to the present invention are omitted for clarity, and like reference numerals designate like parts throughout the specification.

Hereinafter, the present invention will be described with reference to the accompanying drawings.

1 is a flow chart showing a method for manufacturing a cosmetic composition using the extract of the Hwangchil wood according to an embodiment of the present invention.

According to an embodiment of the present invention, a method of preparing a cosmetic composition using the extract of Hwangchil-tree is a first step of immersing Hwangchil-tree in water using distilled water at 45 ° C. to 50 ° C. after washing with water; After the washing is completed to remove the distilled water by drying the yellow lacquer tree, using a mixer to grind the yellow lacquer tree to 200 mesh or less, 150 parts by weight of ethanol based on 100 parts by weight of the yellow lacquer tree using an ultrasonic cleaner for 5 minutes to A second step of performing sonication for 7 minutes; After the sonication is completed by heating to 80 ℃ to remove the ethanol and dried, the third step of producing the extract from the yellow lacquer wood by supercritical extraction; A fourth step of preparing the fermented hwangchil wood extract by fermenting the hwangchil wood extract; A fifth step of adding a first additive made of a natural substance to the extract of the hwangchil wood; A sixth step of preparing the first mixture by first aging for 6 to 8 hours with stirring at 80 rpm to 100 rpm at a temperature of 35 ° C. to 40 ° C. after the addition of the first additive; And a seventh step of preparing a second mixture by adding the herb extract to the first mixture.

Hwangchil-tree is a subtropical evergreen tree, and it is reported to grow on the southwestern coast of Jeollanam-do and Halla-san of Jeju Island such as Wando, Bogil-do and Haenam (Cho Jong-soo, 1990; Choi Byung-wook, 2002). As far as is known in relation to Hwangchil-tree, it has been disclosed as a raw material for paints used in crafts. It has anti-cancer activity in the leaf extract fraction of Hwangchil-tree and its slightly weaker antioxidant activity than α-tocopherol in the Hwangchil-tree extract fraction. . In addition, the aroma component of Hwangchil-tree has a calming effect and a tonic effect on the nervous system, and the antioxidant activity of Hwangchil-tree has been reported to have higher antioxidant effect than the stem (Moon Chang-gon, 2006).

In the first step, the yellow lacquer tree can be washed. At this time, the hwangchil wood may be divided into fruits, leaves and roots, preferably the hwangchil wood may use a root. In the first step, the roots of Hwangchil-tree can be washed with water. After removing soil, dust and the like using water, it is cut to have a length of approximately 2 cm to 5 cm. The cut hwangchil wood may be immersed in distilled water of 45 ℃ to 50 ℃ for 1 hour. Distilled water may be absorbed into the internal structure of the yellow lacquer tree to soften the yellow lacquer tree tissue, and the extraction of the active ingredient may be efficiently performed. If the temperature of the distilled water is less than 45 ℃, it takes a long time when the distilled water penetrates into the internal structure of the yellow lacquer tree, and if the temperature of the distilled water exceeds 50 ℃, the process of crushing the yellow lacquer tree in the subsequent process is not easily performed Do not.

The hwangchil wood may use the root, the root plant is a problem because the residual pesticides in the soil can be concentrated. On the other hand, the Hwangchil wood extract according to the present embodiment can remove the residual pesticide through the ultrasonic treatment in the second step. In addition, by maintaining the hwangchil wood in distilled water at 45 ℃ to 50 ° C for 1 hour in the first step performed before the second step, it is possible to deeply penetrate the moisture into the cell structure of the hwangchil wood, thereby The ultrasonic treatment can effectively remove the residual pesticides that may remain inside the hwangchil wood. In addition, in the second step, the hwangchil wood can be pulverized to a size of 200 mesh or less, by making the hwangchil wood to 200 mesh or less, it is possible to more effectively perform the ultrasonic cleaning performed in the second step.

In the third step, the supercritical extraction is performed in a supercritical extraction tank having a pressure of 400 bar and a temperature of 50 ° C., and a separation tank having a pressure of 40 bar and a temperature of 40 ° C., and a flow rate of ethanol as a cosolvent is 5 per minute. It can be carried out for 2 hours by adding to ml.

In the third step, the hwangchil wood extract may be prepared by supercritical extraction. The conventional method of extracting hot water using water or the like has a problem that it is difficult to sufficiently extract the functional material contained in the yellow lacquer tree, and it takes a long time to extract it. In addition, some of the active ingredient of the hwangchil wood also caused a problem of inert at high temperatures. On the other hand, in the present embodiment, the hwangchil wood extract may be prepared by supercritical extraction. The supercritical extraction can efficiently produce the hwangchil wood extract, and at the same time it is possible to prevent the destruction of the functional material contained in the hwangchil wood because high temperature heat is not used. In addition, it can further promote the activation of the active ingredient contained in the yellow lacquer tree.

In the fourth step, the yellow lacquer extract fermentation is added 600 parts by weight of distilled water with respect to 100 parts by weight of the yellow lacquer extract extract, the lactic acid bacteria culture medium to 5 parts by weight based on 100 parts by weight of the yellow lacquer extract Can be added and fermented at 36 ° C. for 2 weeks.

The lactic acid bacteria culture solution may be obtained by adding 4 g of co-enzyme alpha-herbzyme (α-herbzyme) to 50 g of the yellow lacquer tree powder and contacting with lactic acid bacteria for 3 days at 37 ° C.

The hwangchil wood extract is fermented using lactic acid bacteria can be prepared as a hwangchil wood extract fermentation. The fermentation extract of the Hwangchil wood is functional ingredients and antioxidant power is further improved by the fermentation, when used as the cosmetic composition, the whitening effect can be more effectively exhibited.

The lactic acid bacteria ferment the extract of Hwangchil, thereby increasing the active ingredient and physiological activity contained in the Hwangchil wood extract. For example, the lactic acid bacteria are matrix metalloproteinase-1 (MMP) -1 that degrades skin constituent proteins and the expression of procollagen type I (COL1A1), which is involved in sulfate activity and collagen synthesis, and tumor necrosis that induces skin inflammation. It can improve the inhibitory effect of factor (TNF) -α.

The lactic acid bacteria, Lactobacillus sakei (Lactobacillus sakei B2-16), Lactobacillus brevis (Lactobacillus brevis), Lactobacillus plantarum P23, Lactococcus lactis (Lactococcus lactis A164) and Bacillus coagulus (Bacillus coagulance) may be any one or more selected from the group consisting of. You can also add Saccharomyces cerevisiae strain to yeast Saccharomyces cerevisiae.

The inoculation amount of the lactic acid bacteria or yeast is preferably 10 ⁵ to 10 ⁸ cfu / g or 10 ⁵ to 10 ⁸ cfu / ㎖ initial inoculation, when the initial inoculation bacteria is less than 10 ⁵ cfu / g or 10 ⁵ cfu / ㎖ There is a problem in that the fermentation time is long, and when the amount exceeds 10 ⁸ cfu / g or 10 ⁸ cfu / ㎖ there is a problem that the inoculation amount is excessive, even if the increase in effect is not large.

In the fifth step, the first additive may include Centella asiatica extract and Angelica Root Extract.

Centella asiatica L. Urban is native to the tropics of the Indian Ocean. About 50 species are found in the Centella species and the most common is C. astiatica, which is known to live in wetlands in India, Sri Lanka, Africa and China. Verma et al. 1999; al. 2003). Centella as an antioxidant activity (Pittella et al. 2009; Ariffin et al. 2011), antibacterial activity (Zaidan et al. 2005; Taemchuay et al. 2009; Bibi et al. 2011; Lalitha et al. 2013; Idris and Nadzir 2017) , Oxidative stress alleviation effect by heavy metals (Flora and Gupta 2007), anticancer effect against various cancers (Babykutty et al. 2009), neurostable effect (Krishnamurthy et al. 2009; Orhan 2012), enhancement of immune activity (Keon et al. (2008; Ha et al. 2009) and other studies on various bioactive functions have been reported. In addition, collagen synthesis efficacy (Kim 2010; Hashim et al. 2011), anti-inflammatory and scalp improvement (Jo et al. 2014), lip wrinkle improvement (Suh et al. 2007), keratinocyte growth (Kim et al. Kim, 1995) and skin regeneration (Bylka et al. 2014) have been reported to have an excellent effect on skin beauty (Bylka et al. 2013).

Angelica gigas (AG) Radix is the dried root of perennial herb belonging to the family Umbelliferae, and is distributed in Korea, China, and Japan. Dongguk, which grows in Korea, grows in the mountainous highlands of the northern and northern parts of Korea at 200 ~ 1500m above sea level in Pyeongchang, Inje, Hongcheon, Taebaek, and Jeonbuk Muju (Yu et al., 2004). The flowers of Angelica are purple, and they are grouped in a double inflorescence in August-September. Fruits are oval, with wide wings at the edges, slightly bitter, with an unusual aroma. Angelica is known to be effective in circulating blood in the body in oriental medicine and supplementing insufficient blood to strengthen menstruation or to strengthen the uterus before and after childbirth (Kim et al., 2004). The pharmacologically active ingredients of Angelica gigas include decursin, decursinol angelate, isoimperatonin, nodakenin, and β-sitosterol. Among them, more than 90% of coumarin derivatives decursin and decursinol angelate are reported (Han et al. , 1998).

The first additive may be made of nanoparticles by preparing the Centella asiatica extract, by preparing the Angelica Angelica Root Extract, by mixing the Centella asiatica and Angelica Angelica Root Extract.

Preparation of the centella extract, after washing the centella is hot-air dried at 40 ℃ and powdered to a diameter of 10 ㎛ to 0.5mm by using a blender, ethanol 1000 based on 100 parts by weight of the centellae in the powdered centella Ultrasonic extraction can be performed for 30 minutes by weight. Subsequently, after ultrasonic extraction was completed, centrifugation was performed at 3000 rpm for 10 minutes, the supernatant was filtered with a 0.45 μm syringe filter, and then concentrated with a reduced pressure concentrator. After concentration was completed, the liquid Centella extract was concentrated in a freezer at -70 ° C. for 24 hours. After storage for a powdered centrifugal extract can be prepared by lyophilized powder.

The extract of the Angelica Root Extract, after washing the Angelica Root in water and dried for 10 days in a cool place, and the 1000 mg of ethanol on the basis of 100 parts by weight of the Angelica Root in 100 rpm After stirring for 3 days once a day in a shaking incubator at 25 ℃ with stirring, the supernatant and the precipitate was filtered and concentrated in a vacuum concentrator. Subsequently, after the concentration is completed, the liquid Angelica ginseng root extract may be stored in a freezer at -70 ° C. for 24 hours, and then powdered by a lyophilizer to prepare a powdered Angelica ginseng root extract.

Since the Centella asiatica extract and Angelica Root Extract can be stored in powder form by lyophilization and thus can be stored for a long time without deterioration. In addition, the fermentation extract of the Hwangchil wood fermented by using lactic acid bacteria, so that it is preferable to manufacture when used in order to maximize its performance, Hwangchil wood extract fermentation by manufacturing so as to allow long-term storage of the Centella extract and Angelica Root Extract Cosmetic compositions containing water can be made easier to mass production.

To prepare the nanoparticles by mixing the Centella asiatica extract and Angelica Root Extract, adding lecithin and cholesterol in a molar ratio into the reactor, chloroform and methanol, and stirring at 100 rpm to 200 rpm for 3 hours to dissolve lecithin and cholesterol, Lipid layer may be prepared by drying at 40 ° C. for 4 hours with a vacuum concentrator until chloroform and methanol are completely removed. The powdered Centella asiatica extract and Chalcedony root extract were dissolved in distilled water at a ratio of 1: 1 (weight ratio) to prepare a concentration of 10 mg / mL, and then, the lipid membrane was placed in a reactor prepared at room temperature using an ultrasonic grinder for 1 hour. Can be made into nanoparticles by homogenization.

The first additive may be nanoparticles in which the Centella asiatica extract and Angelica Root Extract are mixed. Preferably, the first additive may have a particle size of 50 nm to 1000 nm. By preparing the first additive by mixing with the Centella asiatica extract and the Angelica Root Extract, the effect by the Centella asiatica extract and the effect by the Angelica Root Extract can be generated uniformly for a long time, some part in the cosmetic composition Only the effect of the extract is present, and in some, it can be uniformly mixed so that the minute imbalance does not occur that the effect by the true donkey root extract. In addition, the first additive may be provided in the size of the nanoparticles, less than 50nm is difficult to produce a uniform particle size problem, when more than 1000nm where the effect of the Centella extract and Angelica Root Extract deep into the skin Difficult to penetrate the problem.

The cosmetic composition according to the present embodiment may be mixed with Centennial extract fermentation extract and the first additive, Centella asiatica, Chalcedony root extract, which may act on skin whitening, sulfate, and the like. The fermentation extract of the Hwangchil wood is provided with a relatively larger particle size than the additive to be absorbed in the dermal layer of the user to further improve the skin whitening effect and to prevent the formation of blemishes such as newly generated blemishes.

In addition, the Centella asiatica extract and the Angelica Root Extract, which are mixed with the first additive, are provided as nanoparticles, so that they can penetrate deep into the base of the skin to activate collagen production in the skin. In addition, the function of supporting the whitening effect by the extract from the yellow lacquer tree, it is possible to prevent the generation of blemishes such as blemishes that can occur deep inside.

That is, the cosmetic composition according to the present embodiment uses the first additive together with the extract from the yellow lacquer tree, but the production method is different so that the effect of the extract from the lacquer tree and the effect by the first additive interact with each other. As well as skin whitening, it can be more effective to suppress skin aging, improve the damaged skin, and the like.

In the seventh step, the herbal extract contains tea tree oil.

The herbal extract, after mixing 25ml to 50ml of the tea tree oil with respect to 1000ml of distilled water and stirred for 30 minutes at 250rpm at room temperature, after the stirring is completed to reduce the particle size of the tea tree oil mixed in the distilled water The high pressure emulsification process can be carried out at 1,000 bar and 3 cycles to homogenize and homogenize. Subsequently, after completion of the high pressure emulsification process, ultrasonic waves having a frequency of 65 kHz to 75 kHz may be applied at a temperature of 80 ° C. for 2 to 3 hours.

The herbal extract may be included in the cosmetic composition to increase the shelf life while reducing the content of chemicals in the cosmetic composition consisting of natural substances.

The tea tree oil may have an antibacterial effect on the skin, and the herbal extract may reduce particle size through high pressure emulsification. In general, tea tree oil is present in an oil phase (oil phase) is provided as a relatively large particles have a disadvantage that the skin absorption is slower and thereby the effect is not fully exhibited. On the other hand, in the herbal extract according to the present embodiment, the tea tree oil reduces the particle size through high pressure emulsification, and the skin permeability of the active substance of the herbal extract is improved, thereby improving the effect on the skin.

The tea tree oil used as the herbal extract provides antimicrobial properties, thereby preventing the growth of mold or bacteria and the like so that the yellow-fermented tree extract fermentation and natural substance extracts can be maintained for a long time without deterioration. By applying ultrasonic waves having a frequency of 65kHz to 75kHz in the hub extract, it is possible to maintain the tea tree oil emulsified for a long time without being separated from distilled water at room temperature.

According to another aspect of the present invention, the present invention includes a cosmetic composition using the extract from the Hwangchil wood produced by the method described above.

In addition, the cosmetic composition may further include one or more additives selected from the group consisting of antioxidants, stabilizers, solubilizers, vitamins, pigments, flavors and carriers.

For example, the cosmetic composition may be prepared in one or more formulations selected from lotion, nourishing cream, eye cream, moisture cream, lotion, pack, essence.

Hereinafter, examples and comparative examples of the present invention are described. However, the following examples are only preferred embodiments of the present invention and the scope of the present invention is not limited by the following examples.

Production Example

1. Centella extract is manufactured

Centella is washed several times with water from a farmhouse in Hapcheon-gun, Gyeongnam, and washed several times with water, dried at 40 ° C, ground finely in a blender, weighed 10 times with 50% ethanol, and extracted at 30 rpm for 10 minutes. Centrifuged for a minute and the supernatant was filtered through a 0.45 μm syringe filter. Subsequently, the resultant was concentrated in a rotary vacuum concentrator (R-1001, Eyela, Japan), and then the extract of the liquid Centella was prepared in powder at a freeze dryer (FD5508, Korea) after storing for 24 hours in a freezer at -70 ℃.

2. Preparation of Angelica Root Extract

The mulberry root was washed several times in water and dried for 10 days in a cool place out of direct sunlight. Extraction was performed by adding 10 times 50% ethanol to the Angelica Root root and stirring it at 100 rpm for 3 days once a day in a shaking incubator at 25 ° C. After filtering the supernatant and the precipitate, a rotary depressurizer (R- 1001, Eyela, Japan). Subsequently, after the concentration was completed, the liquid donkey root extract was stored for 24 hours in a freezer at -70 ℃ and then prepared as a powder by a freeze dryer (FD5508, Korea).

3. Preparation of the First Additive

Lecithin (Sigma Chemical Co., USA) and Cholesterol (Sigma Chemical Co., USA) were added to the reactor in a molar ratio, followed by chloroform (Sigma Chemical Co., USA) and 50% methanol (Sigma Chemical Co., USA). Stir at 100 rpm to 200 rpm for 3 hours to dissolve lecithin and cholesterol, and dry for 4 hours at 40 ° C with a rotary vacuum concentrator (R-1001, Eyela, Japan) until the chloroform and methanol are completely removed. ) Was prepared. 50g of Centella asiatica extract and 50g of Angelica Root Extract prepared in powder form were dissolved in distilled water and prepared at a concentration of 10mg / mL, and then placed in a reactor in which a lipid membrane was prepared, using an ultrasonic mill (S150, Fisher scientific, Mexico) at room temperature. Homogenization was carried out for 1 hour at to prepare a first additive which is a nanoparticle.

4. Preparation of Herb Extracts

50 ml of the tea tree oil was mixed with 1000 ml of distilled water, followed by stirring at 250 rpm for 30 minutes at room temperature. After performing the high pressure emulsification process (M110H-30, Microfluidics, USA) at 3 cycles at 1,000 bar, 2 hours using an ultrasonic device (S150, Fisher scientific, Mexico) with a frequency of 65 kHz to 75 kHz at a temperature of 80 ℃ Ultrasound was applied.

Preparation of Examples and Comparative Examples

Example 1

Hwangchil-tree was purchased and used in Jangheung-gun, Jeollanam-do. Hwangchil trees were selected only for the roots and washed several times with running water. Then, the cut was made to be approximately 5 cm in length. The cut yellow chile was put in distilled water at a temperature of 45 ℃ was maintained for 1 hour. After removing the hwangchil wood, placed on a sieve and remove the distilled water and dried in the shade for 24 hours. The dried hwangchil wood was put into a blender and pulverized, and the size was selected using a 200 mesh mesh network. 1 kg of hwangchil wood of 200 mesh or less was put in 15 kg of 50% ethanol at room temperature and sonicated for 7 minutes using an ultrasonic cleaner (OMAX Cancer Precision Precision Co., Ltd.), Ultrasonic Cleaner (Model: AJC-4020, Korea). Sonication was performed using ultrasonic waves at a wavelength of 40 kHz, and the output power was approximately 300 Watts, followed by removing ethanol using a sieve, and then heating to 80 ° C. for 10 minutes to remove ethanol. Using supercritical extraction equipment (Ari instrument, Namyangju-si, Gyunggi-do, Korea), the supercritical extraction tank has a pressure of 400bar and a temperature of 50 ° C. In a separation tank, the pressure is 40bar and the temperature is 40 ° C. In addition, the flow rate of the co-solvent ethanol was added to 5 ml per minute to perform supercritical extraction for 2 hours to prepare the Hwangchil wood extract was used as part of the lactic acid bacteria culture solution as follows. Separately, 600 g of distilled water was added to 100 g of the prepared Hwangchil-tree extract, 5 g of lactic acid bacteria culture medium was added, and fermented at 36 ° C. for 2 weeks to prepare a fermentation solution of Hwangchil-tree extract. Lactobacillus extract was prepared by freeze-drying (FD5508, Korea) freeze-dried as a yellow lacquer powder, and then 4 g of co-enzyme alpha-herbzyme was added to 50 g of sulfur lacquer powder. bacteria) were contacted and incubated for 3 days at 37 ° C. 30 g of nanoparticles (first additive) consisting of Centella asiatica extract and Angelica rhizome root extract prepared by the above-described method were added to 100 g of the extract of the fermented hwangchil tree. The mixture was stirred at 100 rpm for 6 hours at 35 ° C. While maintaining the temperature of 35 ° C., the herbal extract prepared by the above-described method was added thereto, and the mixture was stirred at 100 rpm again for 6 hours, and the temperature was maintained. It was reduced to room temperature, stirred for 24 hours to 80rpm, and it was prepared in the freeze-dryer in the functional mixture to a powder-form (FD5508, Korea). Using the prepared functional mixture was prepared as a lotion in the configuration as shown in Table 1.

ingredient content Triethanolamine 0.12㎏ Sodium hyaluronate 3.5 kg 1,3-butylene glycol 2.4 kg glycerin 4.0 kg Carboxyl Vinyl Polymer 0.12 kg Tocopherol Acetate 0.5 kg Dimethicone 0.8 kg Trioctanoine 2.0 kg Squalane 3.0 kg Mineral oil 4.0 kg Cured vegetable oil 1.0 kg Glyceryl Stearate 1.5 kg Stearyl alcohol 1.5 kg lanolin 1.5 kg Polysorbate 1.3 kg Distilled water 72.15ℓ Functional Blend of Example 1 0.6 kg

Comparative Example 1

Comparative Example 1 was prepared in the same manner as in Example 1 except for extracting by using ethanol instead of ultrasonic extraction when extracting the Hwangchil wood extract. Extraction using ethanol was put into 10kg of 50% ethanol in 1kg of Hwangchil wood and immersed for 24 hours at room temperature and centrifuged at 300rpm for 10 minutes using a centrifuge (HRT-620v, Hanil Industrial Co., Korea), Filtering with Whatman # 2 filter paper to separate the supernatant and the precipitate to prepare the Hwangchil wood extract. 30g of the nanoparticles (first additive) consisting of Centella asiatica and Angelica Root Extract prepared by the above-described method was added to 100 g of the Hwangchil-tree extract, which was stirred at 35 ° C. and 100 rpm for 6 hours. Maintaining the 35 ℃, the herbal extract prepared by the above-described method was added and stirred again at 100rpm for 6 hours, the temperature was reduced to room temperature and then stirred at 80rpm for 24 hours, which was powdered with a freeze dryer (FD5508, Korea) Functional mixtures were prepared. Comparative Example 1 was prepared in Table 1 by adding 0.6kg instead of the functional mixture of Example 1 in Table 1.

Comparative Example 2

Hwangchil-tree was purchased and used in Jangheung-gun, Jeollanam-do. Hwangchil tree was selected only the roots, washed several times with running water, dried for 24 hours in the shade and cut into 5cm. 10 kg of 50% ethanol was added to 1 kg of the cut Hwangchil wood and immersed at room temperature for 24 hours, and then centrifuged at 300 rpm for 10 minutes using a centrifuge (HRT-620v, Hanil Industrial Co., Korea). Whatman) # 2 was filtered through a filter paper to separate the supernatant and the precipitate to prepare a Hilchi chinensis extract. Comparative Example 2 was prepared in Table 1 by adding 0.6kg instead of the functional mixture of Example 1 in Table 1.

Evaluation of Examples and Comparative Examples

1. Determination of total flavonoid content

In order to find out the flavonoid content, which is known to exhibit various functionalities among phenolic compounds, it was measured by modifying the method of Moreno et al. (Moreno et al., 2000). 100 μL of the sample solution dissolved in 100% methanol, 20 μL of 10% aluminum nitrate, 20 μL of 1M potassium acetate, and 860 μL of methanol were reacted for 40 minutes, and then absorbance was measured at 415 nm. Rutin 0 to 500 μg / mL as a standard material was prepared in the same manner as the standard calibration curve was prepared and the total flavonoid content in mg / g are shown in Table 2.

Example 1 Comparative Example 1 Comparative Example 2 Flavonoid (mg / g RE ¹⁾ ) 192.09 ± 7.51 ²⁾ 87.35 ± 7.23 56.12 ± 4.25

¹⁾ RE: rutin equivalent.

²⁾ Values are mean ± SD (n = 6).

Referring to Table 2, it could be confirmed that Example 1 contains more flavonoid content than Comparative Example 1. This Example 1 was extracted by using the supercritical extract of the Hwangchil wood, and proceeded to fermentation using lactic acid bacteria, whereby it is determined that the flavonoid content contained more than Comparative Example 1. On the other hand, in the case of Comparative Example 2, compared with Comparative Example 1, the first additives (Centella extract, Angelica Root Extract) and herb composition is not included, and it is determined that the flavonoid content is lower than that of Comparative Example 1 due to these effects. do.

2. Tyrosinase Inhibitory Activity

Tyrosinase inhibitory activity to confirm the whitening effect in skin is described by Yagi et al. It was carried out in accordance with the method of (1986). To 1.9 mL of a solution of L-tyrosine (0.3 mg / mL) dissolved in 0.1 M of Potassium phosphate buffer (pH 6.8), 0.1 mL of the sample prepared by concentration and 0.1 mL of mushroom tyrosinase (1,250 units / mL) were added. Incubate for 15 min. After that, the enzyme reaction was stopped and the absorbance was measured at 475 nm using a spectrophotometer. Mushroom tyrosinase inhibitory activity was expressed as a percentage of the absorbance reduction rate of the sample solution addition group and no addition group and are shown in Table 3.

Tyrosinase activity (475 nm) Concentration (μg / g) Example 1 Comparative Example 1 Comparative Example 2 Mean ± SD % of control Mean ± SD % of control Mean ± SD % of control 10 1.751 ± 0.061 77.5 *** 1.902 ± 0.059 80.2 *** 2.028 ± 0.061 82.7 *** 100 1.494 ± 0.038 65.7 *** 1.734 ± 0.041 75.6 *** 1.862 ± 0.038 76.4 *** 500 0.845 ± 0.017 34.3 *** 0.915 ± 0.015 40.2 *** 1.035 ± 0.017 41.2 *** 1000 0.660 ± 0.027 28.4 *** 0.870 ± 0.021 37.5 *** 2.005 ± 0.027 398 ***

Values are mean ± SD of three independent experiments. *** p / 0.001 significantly different from the control (2.341 ± 0.024).

Referring to Table 3, when the tyrosinase inhibitory activity test at a concentration of 10 ~ 1,000 μg / mL, it was confirmed that Example 1 is superior to Comparative Example 1 and Comparative Example 2. On the other hand, Example 1 was not significantly different from Comparative Example 1, it could be confirmed that it appears much higher than Comparative Example 2. In Example 1 and Comparative Example 1, since the first additive is further included, it is determined that tyrosinase inhibitory activity is higher than that of Comparative Example 2, which is composed solely of Hwangchil-tree extract. In addition, considering that the present experiment is not a tyrosinase inhibitory activity using melanocytes but a mushroom tyrosinase inhibitory activity using a reagent, the particle size is reduced to about 1/10 by nanoparticles of the first additive, and thus the nanoparticles are compared with the extracts. It is expected to increase the permeability to skin cells and effectively play a role as a skin whitening activator.

3. Measurement of DPPH Free Radical Scavenging Activity

Scavenging activity against DPPH radical was measured by modifying Blois' method. 100 μL (0.4 mM) of DPPH solution dissolved in ethanol and the same amount were added to a 96 well plate and reacted at 37 ° C. for 30 min. After the reaction, the absorbance was measured at 517 nm to measure DPPH radical scavenging activity, which is shown in Table 4 and FIG. 2. BHT was used as a comparative material.

DPPH free radical scavenging activity Example 1 87.36% Comparative Example 1 57.21% Comparative Example 2 24.85%

Referring to Table 4 and Figure 2, it can be seen that Example 1 is more sulfate activity than Comparative Examples 1, 2. In particular, when comparing Example 1 and Comparative Example 1, it was confirmed that Example 1 is more excellent, which with the difference in the method of extracting the Hwangchil wood, in Example 1 fermentation of the Hwangchil wood extract using lactic acid bacteria It is judged to be used.

4. Antibacterial activity evaluation

Antimicrobial activity was evaluated by direct smear and visual inspection, and the test strains were E. coli and C. albicans, a dermatitis-inducing fungus. The test method is to adjust the bacteria incubated in YM bath for 24 hours to the concentration of # 0.5 McFarland standard, and then spread the cultures on each plate medium, dried for 3 to 5 minutes, and then The plate soaked with was placed on the medium and completely contacted and then examined after 28 days, 1 day, 3 days, and 7 days, and the results are shown in Table 5.

division Test strain 0 days 1 day 5 days 10 days Example 1 Escherichia coli 2.0 * 10 ⁷ 0 0 0 Candida albicans 2.0 * 10 ⁷ 8 0 0 Comparative Example 1 Escherichia coli 2.0 * 10 ⁷ 0 0 0 Candida albicans 2.0 * 10 ⁷ 7 0 0 Comparative Example 2 Escherichia coli 2.0 * 10 ⁷ 0 5 25 Candida albicans 2.0 * 10 ⁷ 12 21 25

Referring to Table 5, Example 1 and Comparative Example 1 can be seen to show a strong antimicrobial effect against the two strains commonly used in the antimicrobial activity test of cosmetics. In particular, by maintaining the antimicrobial effect for more than one week, it appears that there is a very good effect on the alleviation of the symptoms of the skin infection caused by the skin scratch and inhibiting bacterial growth. On the other hand, Comparative Example 2 was confirmed that the antimicrobial activity is very low. Example 1 and Comparative Example 1, Comparative Example 1, and the lotion prepared by Comparative Example 2 does not contain a separate preservatives, while Example 1, Comparative Example 1, unlike Comparative Example 2, and thus further contains the herbal extracts It is believed that antibacterial activity is exerted.

Those skilled in the art will appreciate that the present invention can be embodied in other specific forms without changing the technical spirit or essential features of the present invention. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive. The scope of the present invention is shown by the claims rather than the detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents are included in the scope of the present invention. Should be interpreted.

Claims (12)

Hwangchil wood is washed with water using a first step of immersing in distilled water of 45 ℃ to 50 ℃ for 1 hour;
After washing the dried Hwangchil wood to remove the distilled water, using a mixer to crush the hwangchil wood to a size of 200 mesh or less, based on 100 parts by weight of the Hwangchil wood ethanol 150 parts by weight using an ultrasonic cleaner 5 A second step of performing sonication for minutes to 7 minutes;
After the sonication is completed by heating to 80 ℃ to remove the ethanol and dried, a third step of producing a yellow chile extract by supercritical extraction of the dried yellow chile;
A fourth step of preparing the fermented hwangchil wood extract by fermenting the hwangchil wood extract;
A fifth step of adding a first additive made of a natural substance to the extract of the hwangchil wood;
A sixth step of preparing the first mixture by first aging for 6 to 8 hours with stirring at 80 rpm to 100 rpm at a temperature of 35 ° C. to 40 ° C. after the addition of the first additive; And
A seventh step of preparing a second mixture by adding the herb extract to the first mixture;
In the third step,
The supercritical extraction is performed in a supercritical extraction tank having a pressure of 400 bar and a temperature of 50 ° C., and a separation tank having a pressure of 40 bar and a temperature of 40 ° C., and the flow rate of the co-solvent ethanol is 5 ml / min for 2 hours. Is performed during
In the fourth step,
The yellow lacquer extract fermentation, 600 parts by weight of distilled water is added to 100 parts by weight of the yellow lacquer extract extract, and lactic acid bacteria culture medium is added to 5 parts by weight based on 100 parts by weight of the yellow lacquer extract extract at 36 ℃ After fermentation for 2 weeks, the lactic acid bacteria culture solution was added to 4 g of co-enzyme alpha-herbzyme (α-herbzyme) with respect to 50 g of the yellow lacquer powder, and incubated at 37 ° C. for 3 days by contacting with lactic acid bacteria. Obtained,
The lactic acid bacteria are Lactobacillus sakei (Lactobacillus sakei B2-16), Lactobacillus brevis (Lactobacillus brevis), Lactobacillus plantarum P23, Lactococcus lactis (Lactococcus lactis A164) and Bacillus coagulus (Bacillus coagulance) is any one or more selected from the group consisting of,
In the fifth step,
The first additive is composed of Centella asiatica extract and Angelica Root Extract, The first additive is,
Preparing the Centella extract,
To prepare the extract of Angelica Root,
Mixing the Centella asiatica extracts and extracts made of Angelica Root;
Preparation of the centella extract, after washing the centella is hot-air dried at 40 ℃ and powdered to a diameter of 10 ㎛ to 0.5mm by using a blender, ethanol 1000 based on 100 parts by weight of the centellae in the powdered centella Ultrasonic extraction for 30 minutes by weight, centrifugation at 3000rpm for 10 minutes after the ultrasonic extraction is complete, the supernatant was filtered with a 0.45㎛ syringe filter and concentrated with a reduced pressure concentrator, and after concentration was completed Stored in a freezer at -70 ℃ for 24 hours and then powdered with a lyophilizer,
The extract of the Angelica Root Extract, after washing the Angelica Root in water and dried for 10 days in a cool place, and the 1000 mg of ethanol on the basis of 100 parts by weight of the Angelica Root in 100 rpm After stirring three times a day in a shaking incubator at 25 ° C. with stirring, the supernatant and sediment were filtered and concentrated with a reduced pressure concentrator. After the concentration was completed, the liquid Angelica ginseng root extract was -70 ° C. freezer. After storage for 24 hours in the use of the extract extract fermented hwangchil wood powdered by a lyophilizer,
Preparation of nanoparticles by mixing the Centella asiatica extract and Angelica Root Extract, Lecithin and Cholesterol in a molar ratio, chloroform and methanol in a reactor and then stirred at 100rpm to 200rpm for 3 hours to dissolve lecithin and cholesterol, The chloroform and methanol were dried at 40 ° C. for 4 hours until the chloroform and methanol were completely removed, thereby preparing a lipid layer, and the powdered Centella asiatica and Chaff extract were dissolved in distilled water at a ratio of 1: 1. After preparing to a concentration of 10mg / mL, the lipid membrane was put into a reactor prepared by homogenization at room temperature for 1 hour using an ultrasonic mill, to prepare a nanoparticle,
In the seventh step,
The herb extract is made of tea tree oil, the herb extract is mixed with 25 ml to 50 ml of the tea tree oil with respect to 1000 ml of distilled water and stirred at 250 rpm for 30 minutes at room temperature, and after the stirring is completed, the distilled water In order to reduce and homogenize the particle size of the tea tree oil mixed in the mixture, a high pressure emulsification process was performed at 1,000 bar at 3 cycles, and after completion of the high pressure emulsification process, an ultrasonic wave having a frequency of 65 kHz to 75 kHz was performed at a temperature of 80 ° C. for 2 hours. Method for producing a cosmetic composition using the extract of Hwangchil wood applied for 3 hours. delete delete delete delete delete delete delete delete Cosmetic composition using the Hwangchil wood extract fermentation prepared by claim 1. The method of claim 10,
The cosmetic composition is a cosmetic composition using the extract from the Hwangchil wood, characterized in that it further comprises one or more additives selected from the group consisting of antioxidants, stabilizers, solubilizers, vitamins, pigments, flavors and carriers. The method of claim 10,
The cosmetic composition is a cosmetic composition using the extract from the Hwangchil wood, characterized in that it is prepared in one or more formulations selected from lotion, nutrition cream, eye cream, moisture cream, lotion, pack, essence.

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