KR102164692B1 - Cosmetics for skin whitening including mellisa extract and manufacturing method for the same - Google Patents

Abstract

The present invention relates to skin-whitening cosmetics comprising: a skin whitening agent containing a Melissa officinalis extract; a moisturizing agent; a viscosity-increasing agent; and a surfactant. More specifically, the present invention relates to skin-whitening cosmetics, wherein a tyrosinase secretion inhibition rate and a melanin synthesis inhibition rate of the skin whitening cosmetics increase depending on the concentration of the Melissa officinalis extract.

Description

Skin whitening cosmetics containing lemon balm extract and its manufacturing method {COSMETICS FOR SKIN WHITENING INCLUDING MELLISA EXTRACT AND MANUFACTURING METHOD FOR THE SAME}

The present application relates to a skin whitening cosmetic and a method of manufacturing the same.

Since human skin is directly exposed to the external environment among body tissues, it can be most sensitively affected by biological, chemical, and physical stimuli. Human skin is largely composed of the epidermal layer, the dermis layer, and the subcutaneous fat layer, and the most basic role of the skin is to prevent the evaporation of moisture from our body and to prevent the invasion of harmful ingredients from the outside. Since these skins are always exposed from external stimuli, they have various defense capabilities. In addition, it can be said that healthy and clean skin is directly related to human beauty.

Thanks to this social notion, in order to make the skin look white or to prevent the skin from showing dullness, pigmentation, etc., interest in skin whitening cosmetics has recently increased in the cosmetic industry. Such skin whitening cosmetics can suppress the phenomenon that the skin turns red or black through various principles, such as blocking UV rays and suppressing the synthesis of melanin pigments, as well as symptomatic therapy for simply whitening the skin.

Korean Patent Publication No. 10-1282335, which is the background technology of the present application, relates to a composition for improving skin whitening containing lemon balm extract as an active ingredient. The registered patent discloses that the skin whitening effect is improved when the lemon balm is irradiated with ultraviolet rays, but the case of changing the wavelength of ultraviolet rays when preparing the lemon balm extract is not recognized.

The present application is to solve the problems of the prior art described above, and an object thereof is to provide a skin whitening cosmetic and a method of manufacturing the same.

However, the technical problem to be achieved by the embodiments of the present application is not limited to the technical problems as described above, and other technical problems may exist.

As a technical means for achieving the above technical problem, the first aspect of the present application is to form a lemon balm extract by mixing a lemon balm and a solvent, forming a skin whitening agent comprising the lemon balm extract, and the Including the step of forming a skin whitening cosmetics by mixing a skin whitening agent, a moisturizing agent, a viscosity increasing agent, and a surfactant, the tyrosinase secretion inhibition rate and the melanin synthesis inhibition rate of the skin whitening cosmetics are the concentration of the lemon balm extract It provides a method of manufacturing a skin whitening cosmetic that increases depending on.

According to one embodiment of the present application, the forming of the lemon balm extract may include pulverizing the lemon balm, extracting the powder by mixing with a solvent and ethanol, and concentrating the extract. , But is not limited thereto.

According to the exemplary embodiment of the present disclosure, the extracting may be performed under a temperature rising condition of 1° C./min to 20° C./min, but is not limited thereto.

According to the exemplary embodiment of the present disclosure, the tyrosinase secretion inhibition rate may be 40% to 90%, and the melanin synthesis inhibition rate may be 60% to 90%, but is not limited thereto.

According to one embodiment of the present application, the skin whitening agent is buckwheat extract, green barley extract, natural extract, niacinamide, arbutin, ethyl ascorbic acid, ascorbyl tetraisopalmitate. tetraisopalmitate), and combinations thereof may further include, but are not limited thereto.

According to one embodiment of the present application, the natural extract is bee ant odor extract, mulberry extract, hibiscus callus extract, bamboo callus extract, rice cake butter extract, snow juniper extract, hampock flower extract, red sea ginseng extract, sea cucumber extract, civil scented extract, freshwater laver Extracts, and may include those selected from the group consisting of combinations thereof, but are not limited thereto.

According to an exemplary embodiment of the present disclosure, the concentration of the lemon balm extract may be 10 μg/ml to 400 μg/ml, but is not limited thereto.

According to the exemplary embodiment of the present disclosure, the solvent may include one selected from the group consisting of hexane, chloroform, ethanol, water, and combinations thereof, but is not limited thereto.

In addition, the second aspect of the present application is a skin whitening cosmetic comprising a skin whitening agent containing a lemon balm extract, a moisturizing agent, a viscosity increasing agent, and a surfactant, the inhibition rate of tyrosinase secretion of the skin whitening cosmetic and The melanin synthesis inhibition rate is to increase depending on the concentration of the lemon balm extract, provides a skin whitening cosmetics.

The above-described problem solving means are merely exemplary and should not be construed as limiting the present application. In addition to the above-described exemplary embodiments, additional embodiments may exist in the drawings and detailed description of the invention.

According to the above-described problem solving means of the present application, the skin whitening cosmetic manufactured by the method according to the present application has less toxicity to cells, including lemon balm extract, and can suppress the production of melanocytes, thereby having an excellent skin whitening effect. I can.

In addition, in addition to the lemon balm extract, the skin whitening cosmetics prepared by the method according to the present invention include buckwheat extract, green barley extract, bee ant odor extract, mulberry extract, hibiscus callus extract, bamboo callus extract, rice cake extract, snow juniper extract, and juniper extract. , Red sea ginseng extract, sea cucumber extract, earthy mok hyang extract, freshwater laver extract, and other natural extracts are included, so it is not only skin whitening, but also less irritation to the skin, excellent skin soothing effect, and is effective in improving skin troubles.

In addition, the skin whitening cosmetic manufactured by the method according to the present application can suppress the evaporation of moisture within the skin surface cells, thereby suppressing the skin from becoming crumbly.

Moreover, since the step of preparing a lemon balm extract in the method for producing a skin whitening cosmetic according to the present application gradually changes the temperature and the wavelength of the irradiated light, the skin whitening cosmetic can obtain a higher content of ingredients effective for skin whitening. And, the process cost of the manufacturing method can be reduced.

However, the effect obtainable in the present application is not limited to the effects as described above, and other effects may exist.

1 is a graph showing the tyrosinase inhibitory activity of lemon balm extract according to an embodiment of the present application.
2 is a graph showing the cytotoxicity of lemon balm extract according to an embodiment of the present application.
3 is a graph showing the cytotoxicity of lemon balm extract according to an embodiment of the present application.
Figure 4 is a graph showing the melanin synthesis inhibitory ability of lemon balm extract according to an embodiment of the present application.
5 is a graph showing the melanin synthesis inhibitory ability of lemon balm extract according to an embodiment of the present application.

Hereinafter, exemplary embodiments of the present application will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art may easily implement the present application.

However, the present application may be implemented in various different forms and is not limited to the embodiments described herein. In addition, in the drawings, parts not related to the description are omitted in order to clearly describe the present application, and similar reference numerals are attached to similar parts throughout the specification.

Throughout the present specification, when a part is said to be "connected" with another part, this includes not only the case that it is "directly connected", but also the case that it is "electrically connected" with another element interposed therebetween. do.

Throughout this specification, when a member is positioned "on", "upper", "upper", "under", "lower", and "lower" of another member, this means that a member is located on another member. It includes not only the case where they are in contact but also the case where another member exists between the two members.

Throughout the specification of the present application, when a certain part "includes" a certain component, it means that other components may be further included rather than excluding other components unless specifically stated to the contrary.

As used herein, the terms "about", "substantially" and the like are used at or close to the numerical value when manufacturing and material tolerances specific to the stated meaning are presented, and to aid understanding of the present application In order to prevent unreasonable use by unscrupulous infringers of the stated disclosures, either exact or absolute figures are used. In addition, throughout the specification of the present application, "step to" or "step of" does not mean "step for".

In the entire specification of the present application, the term "combination of these" included in the expression of the Makushi format refers to one or more mixtures or combinations selected from the group consisting of the components described in the expression of the Makushi format, and the component It means to include one or more selected from the group consisting of.

Throughout this specification, the description of "A and/or B" means "A or B, or A and B".

Hereinafter, the skin whitening cosmetics of the present application and a method of manufacturing the same will be described in detail with reference to embodiments and examples and drawings. However, the present application is not limited to these embodiments and examples and drawings.

As a technical means for achieving the above technical problem, the first aspect of the present application is to form a lemon balm extract by mixing a lemon balm and a solvent, forming a skin whitening agent comprising the lemon balm extract, and the Including the step of forming a skin whitening cosmetics by mixing a skin whitening agent, a moisturizing agent, a viscosity increasing agent, and a surfactant, the tyrosinase secretion inhibition rate and the melanin synthesis inhibition rate of the skin whitening cosmetics are the concentration of the lemon balm extract It provides a method of manufacturing a skin whitening cosmetic that increases depending on.

The skin whitening cosmetics according to the present application are not only cosmetics that reduce skin pores or make the skin whiter, but also cosmetics that can suppress the synthesis of melanin in the skin, such as adjusting irregular skin color regularly, or reducing skin dullness. it means.

These skin whitening cosmetics include skin whitening agents such as niacinamide, arbutin, Ethyl ascorbic acid, and Ascorbyl tetraisopalmitate, as well as skin conditioners for moisturizing the skin, A humectant that keeps the moisture of the cosmetic, a viscosity increasing agent that increases the viscosity so that the skin whitening cosmetic does not flow down from the skin, a surfactant for mixing the ingredients of the skin whitening cosmetic, and the pH of the skin whitening cosmetic A pH adjuster that adjusts to have weak acidity or weak alkalinity, a chelating agent that prevents a trace amount of metal ions from flowing into the skin whitening cosmetic, a fragrance to make the cosmetic have a scent, and the cosmetic contains all of the ingredients. It is possible to enable and may include a mixed solvent capable of performing various actions such as extraction of the active ingredient.

In this regard, the solvent for causing the cosmetic to have a liquid form generally refers to a substance such as water and ethanol, and is referred to herein as a "mixed solvent". Unless otherwise specified, the description of "solvent" herein refers to the material used when forming the extract, and the description of "concentration of the extract" is a value obtained by dividing the mass of the active ingredient obtained from the extract by the volume of the solvent. it means.

Lemon balm according to the present application is a kind of herb plant, used as a spice or tea material, refers to a plant having a scent similar to lemon. In this regard, lemon balm contains a lot of various ingredients such as polyphenols and rosmarinic acid, and various effects such as decreasing the number of visceral fat cells can be confirmed if lemon balm is consumed consistently.

According to the exemplary embodiment of the present disclosure, the lemon balm may include lemon balm grown for 1 to 24 months, but is not limited thereto.

According to the exemplary embodiment of the present disclosure, the lemon balm extract may include polyphenols and/or flavonoids as an active ingredient, but is not limited thereto.

Polyphenol according to the present application refers to a substance in which a part of sugar produced when plants photosynthesize is converted. The polyphenol may contain two or more hydroxyl groups (-OH) to perform strong antioxidant activity. The polyphenols of the lemon balm extract may contain ferura acid to inhibit the production of melanin pigments. In addition, the flavonoid according to the present application is a kind of polyphenol and refers to a substance associated with the pigment of plants.

When the skin is exposed to ultraviolet rays for a long time, melanin may be produced in the skin. Specifically, skin keratinocytes exposed to ultraviolet rays secrete melanocyte stimulating hormone, and the melanocyte stimulating hormone binds to melanocortin 1 receptor to secrete enzymes such as tyrosinase and TYRP1. The tyrosinase serves as a catalyst to transform tyrosine into L-DOPA (L-3,4-dihydroxyl-L-phenylalanine), and to transform the L-DOPA into L-dopaquinone, and the L-dopaquinone can function as a precursor of melanin. On the other hand, melanin generated from B16-F10 melanocyte cells present in the base layer among skin cells may be delivered to the surface of the skin by the L-dopaquinone.

In this regard, the color of the skin is determined by the molecular structure and color of the melanin, and the melanin serves to inhibit the penetration of the ultraviolet rays into the dermal layer for about 2 months. However, the melanin does not inhibit 100% of ultraviolet rays, and if the ultraviolet rays are continuously received, the color of the skin may be semi-permanently darkened, and various problems such as aging or pigmentation of the skin may occur.

As will be described later, the skin whitening cosmetic according to the present application is intended to suppress the synthesis of melanin and not to irritate skin cells.

According to the exemplary embodiment of the present disclosure, the inhibition rate of tyrosinase secretion and the inhibition rate of melanin synthesis of the skin whitening cosmetic may increase depending on the concentration of the lemon balm extract, but are not limited thereto.

According to the exemplary embodiment of the present disclosure, the inhibition rate of the tyrosinase secretion of the skin whitening cosmetic may be 40% to 90%, and the melanin synthesis inhibition rate may be 60% to 90%, but is not limited thereto.

For example, the tyrosinase secretion inhibition rate is about 40% to about 90%, about 45% to about 90%, about 50% to about 90%, about 55% to about 90%, about 60% to about 90%. , About 65% to about 90%, about 70% to about 90%, about 75% to about 90%, about 80% to about 90%, about 85% to about 90%, about 40% to about 45%, about 40% to about 50%, about 40% to about 55%, about 40% to about 60%, about 40% to about 65%, about 40% to about 70%, about 40% to about 75%, about 40% To about 80%, about 40% to about 85%, about 45% to about 85%, about 50% to about 80%, about 55% to about 75%, about 60% to about 70%, or about 65% days However, it is not limited thereto.

In addition, the melanin synthesis inhibition rate is about 60% to about 90%, about 65% to about 90%, about 70% to about 90%, about 75% to about 90%, about 80% to about 90%, about 85% To about 90%, about 60% to about 65%, about 60% to about 70%, about 60% to about 75%, about 60% to about 80%, about 60% to about 85%, about 65% to about It may be 85%, about 70% to about 80%, or about 75%, but is not limited thereto.

The rate of inhibition of tyrosinase secretion according to the present application refers to the degree of inhibition of the production of L-DOPA and the production of melanin. In addition, the melanin synthesis inhibition rate according to the present application refers to a degree of inhibition of the synthesis of melanin in the skin, but may not have a form consistent with the tyrosinase secretion inhibition rate.

According to the exemplary embodiment of the present disclosure, the lemon balm extract may have a cell viability of 20% to 100%, but is not limited thereto.

The cell viability according to the present application means the degree to which the mitochondria of the cells can survive by the lemon balm extract. Specifically, the dehydrogenase in the mitochondria of the cells is a yellow water-soluble tetrazoliumsalt[3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide] (MTT) as a non-aqueous dark purple MTT formazan crystal. When reduced, the cell viability can be analyzed through the degree to which the crystal absorbs light having a wavelength of 500 nm to 600 nm. It is possible to determine how toxic a specific substance has to cells through the cell viability.

First, lemon balm and a solvent are mixed to form a lemon balm extract.

According to an exemplary embodiment of the present disclosure, the forming of the lemon balm extract includes pulverizing the lemon balm, extracting the pulverized lemon balm powder by mixing it with the solvent and ethanol, and fractionating the extract. It may be included, but is not limited thereto.

According to the exemplary embodiment of the present disclosure, the step of forming the lemon balm extract may further include concentrating the extracted material, but is not limited thereto.

According to the exemplary embodiment of the present disclosure, the lemon balm powder may include crushed lemon balm leaves, roots, stems, fruits, or seeds, but is not limited thereto.

In this regard, the sum of the masses of the solvent and ethanol relative to the mass of the powder may be 15 to 25 times, but is not limited thereto.

A material used when extracting the powder, such as a mixed solution of the solvent and the ethanol, may be referred to as an extraction solution.

According to the exemplary embodiment of the present disclosure, the extraction solution may be pure ethanol, or a mixed solution of more than 30% by volume and less than 100% by volume, and of more than 0% by volume and less than 70% by volume of the solvent, but is not limited thereto.

According to the exemplary embodiment of the present disclosure, the solvent may include one selected from the group consisting of hexane, chloroform, ethanol, water, and combinations thereof, but is not limited thereto.

According to the exemplary embodiment of the present disclosure, the extracting may be performed under a temperature rising condition of 1° C./min to 20° C./min, but is not limited thereto.

According to an exemplary embodiment of the present disclosure, the extracting may include selected from the group consisting of reflux extraction, stationary extraction, ultrasonic extraction, high temperature pressure extraction, low temperature high pressure extraction, and combinations thereof, but is limited thereto. no.

When the extracting step is performed by a reflux extraction process, the solvent in which the active ingredient in the powder is dissolved flows downward by gravity, and when the solvent is evaporated, it is liquefied in the process of moving to the riser to remove the active ingredient from the powder. It is known to have a large energy saving effect, such as being able to be extracted.

In this regard, the extracting may include a stirring condition and an irradiation condition of ultrasonic waves. The stirring and irradiation of the ultrasonic wave may improve the extraction efficiency of active ingredients such as polyphenol from the powder.

According to the exemplary embodiment of the present disclosure, the stirring may be performed at a speed of 10 rpm to 300 rpm, and the ultrasonic wave may have a frequency of 10 kHz to 1 MHz, but is not limited thereto.

According to the exemplary embodiment of the present disclosure, the extracting may be performed under a temperature condition of 60° C. to 100° C. through the heating condition, but is not limited thereto.

When the extracting step performed without going through the heating condition, that is, heating so that the temperature of the solvent and the ethanol is located within the range of 60°C to 100°C, and then mixing and extracting the lemon balm powder, the active ingredient of the lemon balm The reduction of the extract may reduce skin whitening performance.

The temperature raising condition according to the present application means defining the degree to which the temperature rises with time. When extracting a component in a material under elevated temperature conditions, the component is slowly extracted through the cell wall of the material, so that when the component is extracted at a constant temperature, it can be extracted with higher purity.

The step of fractionating according to the present application means a step of separating the extract after mixing with the solvent.

According to an exemplary embodiment of the present disclosure, the concentration of the lemon balm extract may be 10 μg/ml to 400 μg/ml, but is not limited thereto. For example, the concentration of the lemon balm extract is about 10 μg/ml to about 400 μg/ml, about 25 μg/ml to about 400 μg/ml, about 50 μg/ml to about 400 μg/ml, about 75 μg /ml to about 400 μg/ml, about 100 μg/ml to about 400 μg/ml, about 150 μg/ml to about 400 μg/ml, about 200 μg/ml to about 400 μg/ml, about 250 μg/ml To about 400 μg/ml, about 300 μg/ml to about 400 μg/ml, about 350 μg/ml to about 400 μg/ml, about 10 μg/ml to about 25 μg/ml, about 10 μg/ml to about 50 μg/ml, about 10 μg/ml to about 75 μg/ml, about 10 μg/ml to about 100 μg/ml, about 10 μg/ml to about 150 μg/ml, about 10 μg/ml to about 200 μg /ml, about 10 μg/ml to about 250 μg/ml, about 10 μg/ml to about 300 μg/ml, about 10 μg/ml to about 350 μg/ml, about 25 μg/ml to about 350 μg/ml , About 50 μg/ml to about 300 μg/ml, about 75 μg/ml to about 250 μg/ml, about 100 μg/ml to about 200 μg/ml, or about 150 μg/ml, but are limited thereto. It is not.

In this regard, the concentration of the lemon balm extract refers to the concentration of the concentrated lemon balm extract or the lemon balm extract before concentration, and does not refer to the degree to which the skin whitening agent to be described later contains the lemon balm extract.

As will be described later, depending on the type of solvent used to extract the lemon balm extract and the concentration of the lemon balm extract, the whitening performance of the skin whitening cosmetic may vary.

Then, a skin whitening agent containing the lemon balm extract is formed.

According to one embodiment of the present application, the skin whitening agent is buckwheat extract, green barley extract, natural extract, niacinamide, arbutin, ethyl ascorbic acid, ascorbyl tetraisopalmitate. tetraisopalmitate), and combinations thereof may further include, but are not limited thereto. In this regard, the skin whitening agent may include a material that is helpful for skin whitening, such as decolorizing the color of the synthesized melanin or inhibiting the production of melanin by inhibiting the secretion of tyrosinase.

Niacinamide according to the present application refers to vitamin B3, and can have various effects such as shrinking pores, adjusting unbalanced skin tone, and improving skin aging.Arbutin can activate melanin pigment and inhibit skin pigmentation. Ethyl ascorbyl ether and ascorbyl tetraisopalmitate are derivatives of ascorbic acid (vitamin C), and unlike ascorbic acid, they can be soluble in various organic solvents and stably pigmented even when applied to weakly acidic skin. It refers to a substance that has excellent skin whitening effect, such as relieving the skin.

According to one embodiment of the present application, the buckwheat extract, the green barley extract, and the natural extract are reflux extraction, stationary extraction, ultrasonic extraction, high-temperature pressure extraction, low-temperature high-pressure extraction, and a combination thereof by a process selected from the group consisting of It may be formed, but is not limited thereto.

According to the exemplary embodiment of the present disclosure, the skin whitening agent may include 0.1 to 10 parts by weight of the lemon balm extract based on 100 parts by weight of the skin whitening agent, but is not limited thereto. For example, based on 100 parts by weight of the skin whitening agent, the skin whitening agent contains about 0.1 parts by weight to about 10 parts by weight of the lemon balm extract, about 1 part by weight to about 10 parts by weight, about 2 parts by weight to about 10 parts by weight , About 3 parts by weight to about 10 parts by weight, about 4 parts by weight to about 10 parts by weight, about 5 parts by weight to about 10 parts by weight, about 6 parts by weight to about 10 parts by weight, about 7 parts by weight to about 10 parts by weight , About 8 parts by weight to about 10 parts by weight, about 9 parts by weight to about 10 parts by weight, about 0.1 parts by weight to about 1 parts by weight, about 0.1 parts by weight to about 2 parts by weight, about 0.1 parts by weight to about 3 parts by weight , About 0.1 parts by weight to about 4 parts by weight, about 0.1 parts by weight to about 5 parts by weight, about 0.1 parts by weight to about 6 parts by weight, about 0.1 parts by weight to about 7 parts by weight, about 0.1 parts by weight to about 8 parts by weight , About 0.1 parts by weight to about 9 parts by weight, about 1 part by weight to about 9 parts by weight, about 2 parts by weight to about 8 parts by weight, about 3 parts by weight to about 7 parts by weight, about 4 parts by weight to about 6 parts by weight Or may be included as about 5 parts by weight, but is not limited thereto.

In this regard, the balance of the skin whitening agent, that is, 90 parts by weight to 99.9 parts by weight of natural extract, niacinamide, arbutin, ethyl ascorbic acid, ascorbyl tetraisopalmitate (Ascorbyl tetraisopalmitate), and combinations thereof, or a solvent for mixing the skin whitening agent with other substances, but is not limited thereto.

According to one embodiment of the present application, the natural extract is bee ant odor extract, mulberry extract, hibiscus callus extract, bamboo callus extract, rice cake butter extract, snow juniper extract, hampock flower extract, red sea ginseng extract, sea cucumber extract, civil scented extract, freshwater laver Extracts, and may include those selected from the group consisting of combinations thereof, but are not limited thereto.

The mulberry extract according to the present application may contain kazinol F to inhibit the activity of the tyrosinase, and in addition to the mulberry extract, beehive extract, hibiscus callus extract, bamboo callus extract, rice cake bottle extract, juniper extract , Hambak flower extract, red sea ginseng extract, sea cucumber extract, geomok hyang extract, and/or freshwater seaweed extract may have an effect of skin whitening.

The buckwheat extract according to the present application itself does not function as a skin whitening agent, but the buckwheat extract can inhibit aging of cells by performing an antioxidant action, and the green barley extract according to the present application may function as a skin whitening agent.

According to one embodiment of the present application, the lemon balm extract, the natural extract, the buckwheat extract, and the green barley extract may be prepared using all parts of plants, animals, or organisms as raw materials. For example, the plant extract may be extracted from a portion selected from the group consisting of roots, stems, leaves, flowers, fruits, seeds, and combinations thereof of the plant.

In this regard, depending on various factors such as scientific name, variety, and growth environment, the substances contained in living organisms may be partially different. Specifically, the lemon balm may include Melissa officinalis , and the green barley is Secale cereale in Hordeum vulgare . L may be included, the buckwheat may include Fagopyrum esculentum , the oak tree may include Broussonetia kazinoki , the beehive may include Aster koraiensis , and the rose of sharon may include Hibiscus syriacus , , The bamboo may include Sasa coreana Nakai , and the rice cake may include Salix hallaisanensist , and the snow juniper is Juniperus chinensis var. may include sargentii, the Magnolia sieboldii may include the Magnolia sieboldii, the honghaesam may include the Stichopus japonicus Red or Stichopus japonicus Orange, the civil fragrance can contain Inula helenium, the freshwater Kim May include Prasiola japonic , but is not limited thereto. In addition, the sea cucumber may include a creature of the genus Holothuroidea , but is not limited thereto.

According to one embodiment of the present application, based on 100 parts by weight of the skin whitening agent, the skin whitening agent is each independently 0.01 parts by weight to 1 part by weight of beehivele extract, 0.01 parts by weight to 1 part by weight of mulberry extract, 0.01 parts by weight of Mugunghwa callus extract To 1 part by weight, bamboo callus extract 0.01 part by weight to 1 part by weight, rice cake buttle extract 0.01 part by weight to 1 part by weight, snow juniper extract 0.01 part by weight to 1 part by weight, chinensis extract 0.01 part by weight to 1 part by weight, red sea ginseng 0.01 part by weight to 1 part by weight of extract, 0.01 part by weight to 1 part by weight of sea cucumber extract, 0.01 part by weight to 1 part by weight of civil scent extract, 0.01 part by weight to 1 part by weight of freshwater laver extract, 0.01 part by weight to 1 part by weight of buckwheat extract , And 0.1 parts by weight to 5 parts by weight of the green barley extract, but are not limited thereto.

According to the exemplary embodiment of the present disclosure, the step of forming the skin whitening agent may be performed before or after the step of concentrating the lemon balm extract, but is not limited thereto.

For example, the skin whitening agent is the buckwheat extract, the green barley extract, the natural extract, the niacinamide, the arbutin, the ethyl ascorbic acid in the extracted lemon balm extract solution. , And the ascorbyl tetraisopalmitate (Ascorbyl tetraisopalmitate) is added, or the concentrated lemon balm extract is dissolved again in the solvent, and the natural extract, the buckwheat extract, the green barley extract, the niacin amide, the arbutin, After preparing a solution mixed with the ethyl ascorbyl ether and the ascorbyl tetraisopalmitate, it may be obtained by removing the solvent from the solution, such as filtering the solution.

According to one embodiment of the present application, the method of manufacturing the skin whitening cosmetic includes the lemon balm extract, the natural extract, the buckwheat extract, and the green barley extract Lactobacillus acidophilus , Lactobacillus sakei ( Lactobacillus sakei ), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus casei ), Lactobacillus paracasei ( Lactobacillus Paracasei ), and combinations thereof selected from the group consisting of It may further include a step of fermenting by lactic acid bacteria, including, but is not limited thereto.

The lactic acid bacteria according to the present application can assist the skin whitening agent to suppress the production of melanin by providing sufficient nutrients to the skin, improving blood circulation and smoothing metabolism.

According to one embodiment of the present application, the method of manufacturing the skin whitening cosmetic may further include light-treating the lemon balm extract, the natural extract, the buckwheat extract, and the green barley extract with ultraviolet rays, visible rays, or infrared rays. However, it is not limited thereto. In this regard, when the lemon balm powder or the green barley powder is light-treated or the extract is light-treated, the skin whitening effect may be improved.

According to the exemplary embodiment of the present disclosure, the wavelength of light used in the light processing step may be 200 nm to 900 nm, but is not limited thereto. For example, the wavelength of the light is about 200 nm to about 900 nm, about 250 nm to about 900 nm, about 300 nm to about 900 nm, about 350 nm to about 900 nm, about 400 nm to about 900 nm, about 450 nm to about 900 nm, about 500 nm to about 900 nm, about 550 nm to about 900 nm, about 600 nm to about 900 nm, about 650 nm to about 900 nm, about 700 nm to about 900 nm, about 750 nm To about 900 nm, about 800 nm to about 900 nm, about 850 nm to about 900 nm, about 200 nm to about 250 nm, about 200 nm to about 300 nm, about 200 nm to about 350 nm, about 200 nm to about 400 nm, about 200 nm to about 450 nm, about 200 nm to about 500 nm, about 200 nm to about 550 nm, about 200 nm to about 600 nm, about 200 nm to about 650 nm, about 200 nm to about 700 nm , About 200 nm to about 750 nm, about 200 nm to about 800 nm, about 200 nm to about 850 nm, about 250 nm to about 850 nm, about 300 nm to about 800 nm, about 350 nm to about 750 nm, about It may be 400 nm to about 700 nm, about 450 nm to about 650 nm, about 500 nm to about 600 nm, or about 550 nm, but is not limited thereto.

According to the exemplary embodiment of the present disclosure, the wavelength of the light may be changed at a rate of 1 nm/min to 100 nm/min, but is not limited thereto.

For example, the wavelength of the light is about 1 nm/min to about 100 nm/min, about 5 nm/min to about 100 nm/min, about 10 nm/min to about 100 nm/min, about 20 nm/min To about 100 nm/min, about 30 nm/min to about 100 nm/min, about 40 nm/min to about 100 nm/min, about 50 nm/min to about 100 nm/min, about 60 nm/min to about 100 nm/min, about 70 nm/min to about 100 nm/min, about 80 nm/min to about 100 nm/min, about 90 nm/min to about 100 nm/min, about 1 nm/min to about 5 nm /min, about 1 nm/min to about 10 nm/min, about 1 nm/min to about 20 nm/min, about 1 nm/min to about 30 nm/min, about 1 nm/min to about 40 nm/min , About 1 nm/min to about 50 nm/min, about 1 nm/min to about 60 nm/min, about 1 nm/min to about 70 nm/min, about 1 nm/min to about 80 nm/min, about 1 nm/min to about 90 nm/min, about 5 nm/min to about 90 nm/min, about 10 nm/min to about 80 nm/min, about 20 nm/min to about 70 nm/min, about 30 nm /min to about 60 nm/min, or about 40 nm/min to about 50 nm/min, but it is not limited thereto.

In the light processing step, unlike the case where the wavelength of light is kept constant, when the wavelength of light is changed at a constant rate, it was confirmed that more active ingredients were extracted.

In this regard, a condition for changing the wavelength of light at a constant speed in the light processing step may be referred to as a wavelength shift condition, and a condition for maintaining the wavelength of light constant may be referred to as a wavelength maintenance condition.

According to one embodiment of the present application, the step of fermenting by the lactic acid bacteria and the step of light treatment may be performed before or after preparing the lemon balm extract, or before or after preparing the skin whitening agent, but is limited thereto. It is not.

Subsequently, the skin whitening agent, moisturizing agent, viscosity increasing agent, and surfactant are mixed to form skin whitening cosmetics.

The surfactant according to the present application is a detergent for removing contaminants in the skin whitening cosmetics, a solubilizer that transparently dissolves a small amount of oil in a solvent, an emulsifier that allows oil and solvent to be well mixed, and the efficacy of other surfactants by forming bubbles. It may include a foam accelerator or the like to increase the, but is not limited thereto.

According to the exemplary embodiment of the present application, the moisturizing agent may include glycerin, but is not limited thereto.

According to one embodiment of the present application, the skin whitening cosmetic is a viscosity increasing agent including xanthan gum, hydroxyethyl cellulose, and a viscosity increasing agent selected from the group consisting of combinations thereof, and cocamido propyl betaine, sodium chloride, lauryl A surfactant including hydroxy sultaine, decylglucoside, and octyldodeces-16 may be further included, but is not limited thereto.

Xanthan gum according to the present application refers to a high molecular weight heteropolysaccharide gum obtained by pure culture and release of carbohydrates using soybean blight bacteria ( Xanthomonas campestris ). The xanthan gum not only increases the viscosity of the skin whitening cosmetic, but also inhibits separation of components on the skin whitening cosmetic, and provides moisture to the skin.

Hydroxyethyl cellulose according to the present application refers to a modified cellulose polymer component having a hydroxyethyl side chain structure, and is known to have excellent salt resistance and pH stability.

Cocamido propyl betaine according to the present application means an amphoteric surfactant obtained by neutralizing fatty acids obtained from coconut oil with dimethylaminopropylamine, and can be used as a foam accelerator or a viscosity increasing agent.

Sodium chloride according to the present application is to increase the viscosity of the surfactant, and may additionally provide an acidity control and antiseptic function.

Lauryl hydroxy sultaine according to the present application, as an amphoteric surfactant, may additionally play a role of controlling the viscosity of the skin whitening cosmetics.

Decyl glucoside according to the present application is a component obtained by the condensation reaction of decyl alcohol and glucose, and is a nonionic vegetable surfactant extracted from corn, coconut, palm cornel oil, and the like. The decyl glucoside provides skin moisturization and hardly irritates the skin, so it can be used as a skin conditioner.

Octyldodeces-16 according to the present application is a type of surfactant, which is a polyethylene glycol ether of octyldodecanol. The octyldodeces-16 has excellent skin affinity and may help the skin absorb the active ingredient.

In addition, the second aspect of the present application is a skin whitening cosmetic comprising a skin whitening agent containing a lemon balm extract, a moisturizing agent, a viscosity increasing agent, and a surfactant, the inhibition rate of tyrosinase secretion of the skin whitening cosmetic and The melanin synthesis inhibition rate is to increase depending on the concentration of the lemon balm extract, provides a skin whitening cosmetics.

With respect to the skin whitening cosmetics according to the second aspect of the present application, detailed descriptions of parts that overlap with the first aspect of the present application have been omitted, but the content described in the first aspect of the present application is the second aspect of the present application even if the description is omitted. The same can be applied to

According to the exemplary embodiment of the present disclosure, the skin whitening cosmetic may have a form selected from the group consisting of gels, creams, ointments, lotions, packs, and combinations thereof, but is not limited thereto.

The present invention will be described in more detail through the following examples, but the following examples are for illustrative purposes only and are not intended to limit the scope of the present application.

[Example 1]

The leaves and stems of lemon balm ( Melissa officinalis ) fermented by Lactobacillus acidophilus and irradiated with light for 1 hour were crushed. Subsequently, 200 g of the powder was placed in an extraction flask, and 2 L of ethanol and 2 L of chloroform were mixed, and the temperature was increased by 5° C. per minute, followed by reflux extraction twice for 2 hours at 80° C.

In this regard, the initial wavelength of the light was 500 nm, but decreased by 10 nm per minute, and finally became 300 nm. One hour of irradiation was the time taken from 500 nm to 300 nm and the light of 300 nm wavelength. Including the treatment time, the reflux extraction process was performed while stirring at a speed of 60 rpm and irradiating ultrasonic waves at 50 kHz.

Subsequently, the reflux extract was filtered using a filter paper (Whatman No. 2, GE Health care, UK), concentrated under reduced pressure to obtain an extracted concentrate, and then suspended with 100 ml of distilled water.

Then, the suspension and 100 ml of chloroform were mixed and fractionated over 3 hours. The fractionation process was repeated 3 times, and then refrigerated at 4°C.

[Example 2]

Same as Example 1, except that hexane was used instead of chloroform.

[Example 3]

Same as Example 1, but purified water was used instead of chloroform.

[Example 4]

Same as Example 1, but did not perform a separate fractionation step.

[Example 5]

In the lemon balm extract obtained through the process of Example 1, green barley ( Hordeum vulgare ) extract, buckwheat ( Fagopyrum esculentum ) extract, mulberry ( Broussonetia kazinoki ) extract, bee ants ( Aster koraiensis ) extract, hibiscus syriacus extract, Shini Sasa coreana Nakai extract, Salix hallaisanensist extract, Juniperus chinensis var.sargentii extract, Magnolia sieboldii extract, red sea ginseng ( Stichopus japonicus Red or Stichopus japonicus Orange ) extract, civil scent Inula helenium ) extract, freshwater laver ( Prasiola japonic ) extract, and sea cucumber ( Holothuroidea ) extract, niacin amide, arbutin, ethyl ascorbyl ether, ascorbyl tetraisopalmitate, and purified water were mixed to prepare a skin whitening agent.

At this time, with respect to 1 g of the skin whitening agent, 100 mg of lemon balm extract, 10 mg of green barley extract, 10 mg of buckwheat extract, 10 mg of mulberry extract, 10 mg of Mugunghwa extract, 10 mg of Shinidae extract, 10 mg of pickbutel extract, Snow juniper extract 10 mg, Psyllium chinensis extract 10 mg, Red sea ginseng extract 10 mg, Geomok flavor extract 10 mg, Freshwater seaweed extract 10 mg, Sea cucumber extract 10 mg, Niacin amide 20 mg, Arbutin 20 mg, Ethyl ascorbyl ether 10 mg, And 20 mg of ascorbyl tetraisopalmitate and the remaining amount of purified water. Subsequently, glycerol, xanthan gum, hydroxy ethyl, cellulose, cocamido propyl betaine, sodium chloride, lauryl hydroxy sultaine, decyl glucoside, octyldodeces-16, purified water, and ethanol are added to the skin whitening agent. Whitening cosmetics were prepared.

[Comparative Example 1]

The same as in Example 1, but without heating conditions, the lemon balm powder was mixed in a mixture of 2 L of ethanol and 2 L of hexane at room temperature, and then extracted at room temperature for 3 days to prepare a lemon balm extract.

[Comparative Example 2]

Same as Example 1, but the fermentation process by Lactobacillus acidophilus was not performed.

[Comparative Example 3]

Same as Example 1, except that the light treatment process by ultraviolet rays was not performed.

[Comparative Example 4]

The same as in Example 1, except that the fermentation process by Lactobacillus acidophilus and the light treatment process by UV light were not performed.

[Comparative Example 5]

The same as in Example 1, except for 1 hour by irradiating ultraviolet rays of 300 nm wavelength to prepare a lemon balm extract.

[Comparative Example 6]

Same as Example 1, but not stirred during the reflux extraction process.

[Comparative Example 7]

Same as Example 1, except that ultrasonic waves were not irradiated during the reflux extraction process.

[Comparative Example 8]

The same as in Example 5, but to prepare a skin whitening cosmetics containing only the lemon balm extract.

[Comparative Example 9]

The same as in Example 5, but to prepare a skin whitening cosmetics that does not contain the lemon balm extract.

[Comparative Example 10]

The same as in Example 5, except that the lemon balm extract of Comparative Example 4 was used as the lemon balm extract.

[Experimental Example 1]

The contents of polyphenols and flavonoids in the lemon balm extracts of Examples 1 to 4 were analyzed through the Folin-Denis method and Folin-Ciocalteu, and the contents are shown in Table 1 below.

[Table 1]

Referring to Table 1, it can be seen that the content of polyphenols and flavonoids in the lemon balm extract was highest when fractionated with chloroform.

[Experimental Example 2]

1 is a graph showing the tyrosinase inhibitory activity of lemon balm extracts according to Examples 1 to 4.

Referring to FIG. 1, it can be seen that when the lemon balm extract is fractionated, the tyrosinase inhibitory effect tends to increase according to the concentration of the lemon balm extract.

2 and 3 are graphs showing the cytotoxicity of lemon balm extract according to the embodiment. Specifically, FIG. 2 is a lemon balm extract of Example 1, and FIG. 3 is a lemon balm extract of Example 2.

2 and 3, when the solvent used to prepare the lemon balm extract is chloroform, the cytotoxicity increases significantly depending on the concentration of the lemon balm extract, but when hexane is used, the cytotoxicity and the lemon It can be seen that the relationship between the concentration of chestnut extract is small.

4 and 5 are graphs showing the ability to inhibit melanin synthesis of lemon balm extract according to an embodiment of the present application. Specifically, FIG. 4 is a lemon balm extract of Example 1, and FIG. 5 is a lemon balm extract of Example 2.

Referring to FIGS. 4 and 5, it can be seen that as the concentration of the lemon balm extract increases, the amount of melanin biosynthesis is suppressed.

1 to 5, the lemon balm extract of Example 1 has excellent overall skin whitening performance when the concentration is 0.01 μg/ml to 100 μg/ml, and the lemon balm extract of Example 2 has a concentration When is 0.01 μg/ml to 100 μg/ml, it can be seen that the overall skin whitening performance is excellent.

[Experimental Example 3]

Table 2 below shows the performance of the lemon balm extracts of Example 1 and Comparative Examples 2 to 5.

[Table 2]

Referring to Table 2, when a lemon balm extract is prepared at room temperature (Comparative Example 1), when a fermentation process is not performed (Comparative Example 2), and when a light treatment process by ultraviolet rays is not performed (Comparative Example 3). , When the fermentation process and the light treatment process are not performed (Comparative Example 4), when the wavelength is maintained during light treatment (Comparative Example 5), or when not irradiated with stirring or ultrasonic waves (Comparative Example 6 or 7), the heating condition It can be seen that the amount of extraction of polyphenols and flavonoids was reduced compared to the case where both the fermentation process and the light treatment process under wavelength shifting conditions were performed (Example 1).

[Experimental Example 4]

100 adult men and women aged 20 to 50 years old were asked to use the skin whitening cosmetics of Example 5 and Comparative Examples 8 to 10 once a day for one month, and the skin condition before and after use was surveyed and the results The average of is summarized in the form of Table 3 below.

[Table 3]

In Table 3, 1 point means very poor, 2 points are poor, 3 points are normal, 4 points are excellent, and 5 points are very excellent.

Referring to Table 3, rather than using only the lemon balm extract (Comparative Example 8) or using only the aforementioned various extracts and niacinamide (Comparative Example 9), other natural extracts are used together (Example 5) turned out to be more desirable. In addition, referring to Tables 2 and 3 together, it can be seen that skin whitening performance varies depending on the method of forming the lemon balm extract.

The foregoing description of the present application is for illustrative purposes only, and those of ordinary skill in the art to which the present application pertains will be able to understand that it is possible to easily transform it into other specific forms without changing the technical spirit or essential features of the present application. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.

The scope of the present application is indicated by the claims to be described later rather than the detailed description, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be interpreted as being included in the scope of the present application.

Claims (9)

Fermenting lemon balm by Lactobacillus acidophilus lactic acid bacteria, light treatment and pulverization, reflux extraction by mixing the pulverized lemon balm powder with a solvent and ethanol, and fractionating the extract Forming a lemon balm extract comprising a;
Forming a skin whitening agent comprising the lemon balm extract; And
Forming skin whitening cosmetics by mixing the skin whitening agent, moisturizing agent, viscosity increasing agent, and surfactant;
Including,
The wavelength of light used in the step of photo-treating the lemon balm is 200 nm to 900 nm,
The wavelength of light changes at a rate of 1 nm/min to 20 nm/min,
The extracting step includes a heating condition, a stirring condition and an ultrasonic irradiation condition,
The extracting step is carried out under a temperature rising condition of 1 ℃ / min to 20 ℃ / min,
The stirring is performed at a speed of 10 rpm to 300 rpm,
The ultrasonic wave has a frequency of 10 kHz to 1 MHz,
The inhibition rate of tyrosinase secretion and the inhibition rate of melanin synthesis of the skin whitening cosmetic are increased depending on the concentration of the lemon balm extract,
Method of manufacturing skin whitening cosmetics.
delete delete The method of claim 1,
The tyrosinase (tyrosinase) secretion inhibition rate is 40% to 90%, the melanin synthesis inhibition rate is 60% to 90%, the manufacturing method of skin whitening cosmetics.
The method of claim 1,
The skin whitening agent is buckwheat extract, green barley extract, natural extract, niacinamide, arbutin, ethyl ascorbic acid, ascorbyl tetraisopalmitate, and combinations thereof The method of manufacturing a skin whitening cosmetic that further comprises selected from the group consisting of.
The method of claim 5,
The natural extracts include beehives extract, Japanese oak extract, Mugunghwa callus extract, bamboo callus extract, rice cake buttele extract, snow juniper extract, hambak flower extract, red sea ginseng extract, sea cucumber extract, civil scent extract, freshwater seaweed extract, and combinations thereof. The method of manufacturing a skin whitening cosmetic that includes those selected from the group consisting of.
The method of claim 1,
The concentration of the lemon balm extract is 10 μg/ml to 400 μg/ml, a method for producing a skin whitening cosmetic.
The method of claim 1,
The solvent is hexane, chloroform, ethanol, water, and a method for producing a skin whitening cosmetic comprising those selected from the group consisting of combinations thereof.
delete

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