WO2021112297A1 - Cosmetic composition comprising composite fermented product of galactomyces and lichen-derived minerals - Google Patents

Abstract

The present invention provides a cosmetic composition for improving the skin barrier and alleviating ionic imbalance in skin cells, the composition comprising a composite fermented product produced by mixing and culturing galactomyces and lichen-derived minerals.

Description

Cosmetic composition containing lichen-derived minerals and Galactomyces complex fermented product

The present invention relates to a composition for alleviating ion imbalance in cells, and more specifically, improving the skin barrier and improving the skin barrier containing lichen-derived minerals and galactomyces complex fermented products having concentrations of minerals and salts similar to those of extracellular matrix components It relates to a functional cosmetic composition for alleviating ion imbalance.

Minerals, also called minerals, are elements that make up the human body and are not decomposed unlike organic substances that are decomposed when ingested. The minerals that make up the human body are potassium (K), calcium (Ca), selenium (Se), sodium (Na), iodine (I), zinc (Zn), magnesium (Mg), phosphorus (P), sulfur (S), There are chlorine (Cl), copper (Cu), manganese (Mn), iron (Fe), cobalt (Co), etc., which cannot be synthesized in the body and must be consumed through food. It accounts for about 4% of the body and is involved in various functions such as bone and tooth composition, blood oxygen transport, digestion and osmotic pressure regulation.

The minerals are involved in various metabolic processes of the body to maintain a healthy body, but modern people often lack or excessive certain minerals and thus are unbalanced. In particular, in relation to the skin, they suffer from itching of unknown causes, including atopic dermatitis, dry skin, and the like (pruritus, pruritus). The atopic itch is known to be due to histamine being unnecessarily discharged from the skin due to an abnormality in the immune system, but in most cases, it is difficult to clearly identify the cause. It is known that skin-related diseases occur because the skin barrier is damaged and the skin is not moisturized in time and is easily exposed to the attack of external bacteria or viruses.

As above, methods for strengthening the skin barrier to protect skin cells from external bacteria or viruses have been studied, and according to the research results, natural minerals play a role in resolving skin troubles, and do not directly kill viruses or bacteria, but reproduce. It has a function to prevent it from doing so after a certain period of time, and it has been found to have no side effects because it is a natural method. In addition, natural minerals are known to increase skin moisture by activating natural moisturizing factor (NMF) and to prevent skin irritation by activating enzymes that remove active oxygen (SOD).

There is a method of using minerals in skin cells as a component of functional cosmetics by eluting minerals from deep sea water, seaweed, natural minerals, and the like.

On the other hand, lichens are symbiotic complexes of fungi and algae or/and cyanobacteria, and are classified into about 14,000 species worldwide. It was once proposed to use secondary metabolites of lichens as antimicrobial agents or antiherbivore agents. In addition, some lichen extracts have been used as various therapeutic agents in the folklore, and lichen metabolites have antibiotic agent, antimycobacterial, antiviral, analgesic, antipyretic properties, etc. has been shown to have a variety of biological activities. At the same time, research results show that minerals extracted from lichens have an excellent effect in preventing skin aging due to cellular ion imbalance.

On the other hand, the fermented product of the fermented strain was used to improve skin moisturizing. Fermented products are known to have a function of activating natural moisturizing factor (NMF) to supply moisture to skin cells and help form a natural moisturizing barrier, so it is used as a cosmetic ingredient for moisture supply.

As a specific example, among fermented strains, Galactomyces is one of the natural yeasts fermented with yeast. Interest in the effectiveness and various active ingredients contained therein has increased. It started to attract attention as it was contained in the cosmetic brand SK-II products, and various vitamins such as vitamins B1, B2, pantothenic acid, B6, folic acid, carbohydrates, and various amino acids that are natural moisturizing factors that provide skin moisturizing, whitening, pores and soothing effects and rich in various minerals. The anti-aging effect of nucleic acid, which contains more than 7 times that of sardines, is known, and kojic acid, which is known as a representative whitening ingredient, was first known in the early 1900's in the process of fermenting steamed rice (koji) with yeast. On the other hand, various natural enzymes such as saccharification enzymes and proteases produced during the fermentation process are known to have antioxidant, anti-aging, whitening, and skin moisturizing effects by increasing the production and absorption of active substances.

Therefore, the present inventors have made continuous efforts to develop a cosmetic composition that improves the skin barrier and alleviates mineral imbalance from lichen extracts, and as a result, it has been found that natural minerals derived from lichens are composed of various components, and fermented strains Found that galactomyces fermented product contains a large amount of organic matter including natural moisturizing factors, the fermented galactomyces fermented material added with the above lichen-derived minerals has the effect of improving the skin barrier and resolving cellular ion imbalance was found to complete the present invention.

An object of the present invention is to alleviate ionic imbalance in cells, specifically, improving the skin barrier and ion imbalance containing a biological composition similar to the extracellular matrix component, minerals and salts derived from lichen, and a galactomyces complex fermented product. To provide a cosmetic composition for relief.

As a means for solving the above problems,

The present invention provides a cosmetic composition comprising a complex fermented product prepared by mixing and culturing lichen-derived minerals and galactomyces.

In addition, the lichen-derived minerals are Umbilicaria antarctica, Peltigera canina (Peltigera canina), Peltigera praetextata (Peltigera praetextata), Lamarina conduprikans (Ramalina, conduplicans), Ramalina intermedia, Xanthoparmelia farinose, Diplocia canescens, Cetraria aculeate, Cladonia furcata, sudepe Pseudephebe pubescens, Sphaerophorus globosus, Stereocaulon alpinum, Umbilicaria antarctica, Usnea antarctica Any one or more selected from the group comprising , Usnea longissima, Usnea barbata and Usnea aurantiacoatra or Stictanylanderiana It provides a cosmetic composition, characterized in that it is a mineral derived from lichens.

In addition, the lichen-derived minerals are from the group comprising Na, Fe, K, Mg, Al, Mn, Si, Ca, Zn, I, S, P, Se, Mg or pharmaceutically or cosmetically acceptable minerals thereof. It provides a cosmetic composition comprising one or more selected minerals.

In addition, the cosmetic composition provides a cosmetic composition, characterized in that it has the effect of improving the skin barrier and alleviating skin cell ion imbalance.

The cosmetic composition of the present invention contains a mineral complex extracted from lichens and a fermented Galactomyces complex cultured by adding it, thereby improving the skin barrier and alleviating ion imbalance in skin cells. In addition, it has an osmotic balance control of skin cells, activation of gallbladder metabolism, alleviation of inflammation and moisturizing effect.

1 shows Il-6 values generated during hypertonic solution stress to prove the effect of regulating osmotic pressure balance in hypertonic solution according to an embodiment of the present invention.

Figure 2 shows Il-33 values generated during stock solution stress to prove the effect of regulating the osmotic pressure balance in the stock solution according to an embodiment of the present invention.

3 is a graph showing transdermal water loss (TEWL) values for demonstrating the skin barrier effect according to an embodiment of the present invention.

Prior to describing the present invention in detail below, it is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention, which is limited only by the appended claims. shall. All technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art, unless otherwise stated.

Throughout this specification and claims, unless stated otherwise, the term comprise, comprises, comprising is meant to include the stated object, step or group of objects, and steps, and any other object. It is not used in the sense of excluding steps or groups of objects or groups of steps.

On the other hand, various embodiments of the present invention may be combined with any other embodiments unless clearly indicated to the contrary. Any feature indicated as particularly preferred or advantageous may be combined with any other feature and features indicated as preferred or advantageous. Hereinafter, embodiments of the present invention and effects thereof will be described with reference to the accompanying drawings.

The cosmetic composition according to an embodiment of the present invention uses a complex fermented product produced by culturing Galactomyces to which lichen-derived minerals are added as an active ingredient, and when applied to the skin, improves the skin barrier through activation of keratin metabolism, moisturizing and ionization. It provides the effect of improving skin health by alleviating imbalances.

Minerals included in the cosmetic composition according to an embodiment of the present invention are derived from lichens, which are defined as a symbiotic complex of fungi and algae or/and cyanobacteria.

Lichens of the present invention are lichens that are native to the polar or alpine regions, and are Umbilicaria antarctica, Peltigera canina, Peltigera praetextata, Lamarina condu. Freecans (Ramalina, conduplicans), Ramalina intermedia, Xanthoparmelia farinose, Diplocia canescens, Cetraria aculeate, Cladonia pur Cata (Cladonia furcata), Pseudephebe pubescens, Sphaerophorus globosus, Stereocaulon alpinum, Umbilicaria antarctica, Us Includes Usnea antarctica, Usnea longissima, Usnea barbata and Usnea aurantiacotra or Stictanylanderiana, etc. Any one or more lichens selected from the group

The Umbilicaria antarctica is a lichen collected from King George Island in Antarctica by researchers, Usnea longissima is a lichen native to Mt. It is a lichen collected from the alpine region of Yunnan, China. These lichens are a kind of lichen that live in an environment unsuitable for normal living things such as strong and dry winds, irregular fluctuations in moisture and nutrients, and strong ultraviolet rays throughout the year.

In addition, the lichen-derived minerals added to the Galactomyces culture of the present invention refer to minerals, and calcium (Ca), phosphorus (P), potassium (K), iron (Fe), zinc (Zn), copper ( Cu), manganese (Mn), selenium (Se), etc. are contained. Minerals are essential for maintaining healthy cells and the human body, such as improving the skin health of modern people, especially atopic symptoms, and delaying skin aging.

90% of diseases are caused by free radicals, and free radicals in the body promote diseases and skin aging, and destroy cells by making lipids, the main component of cell membrane formation, into lipid peroxides. In the middle-aged and older people, the mineral balance is disrupted and deficient due to a decrease in the intake and absorption of minerals, which inhibits the physiological activity of the skin and interferes with the action of the enzyme (SOD) that removes the active oxygen in the body. As essential components of the enzyme that removes these reactive oxygen species, iron, copper, zinc, selenium, and manganese contained in lichens.

Even when the protective function of the stratum corneum is damaged, the homeostasis function to restore it works. In this case, there are studies that the abundant calcium (Ca) and magnesium (Mg) of lichens can effectively improve it. In addition, calcium (Ca) promotes the production of intercellular lipids and strengthens keratinocytes, and magnesium (Mg) helps transport intercellular substances to the stratum corneum and repairs the stratum corneum. (Mg) has the function of increasing the penetrating pressure and excreting the waste products in the pores, so it is possible to remove old dead skin cells.

In addition, zinc (Zn) plays a very important function in maintaining and regenerating the skin, and selenium (Se) acts as an antioxidant and an essential component of glutathione peroxidase, a type of antioxidant enzyme. It has been reported that it inhibits the production of caine and causes anti-inflammatory action.

In addition, phosphorus (P) is involved in acid-alkali balance within the skin cells and buffers the skin cell fluid. It contributes to the equilibrium, maintains the osmotic pressure inside and outside the skin cells, and resolves the ion imbalance. Another mineral, iron (Fe), binds to oxygen and maintains the metabolism of all tissue cells, and when it is deficient, it causes glossitis, cheilitis, spoon-type nail of the nail and hair loss, and copper (Cu) It activates the melanin-forming enzyme, and when it is deficient, it causes torsion hair, thermophilia nodosa, and bead hair.

In the present invention, "mineral" refers to a mineral extracted from or separated from lichens by a conventional method. In addition, in the present invention, "mineral" refers to a material produced through the extraction process, desalination and filtration process or drying process, and the calcium (Ca), phosphorus (P), potassium (K), iron (Fe), In addition to those referred to as general minerals such as zinc (Zn), copper (Cu), manganese (Mn), and selenium (Se), vitamins, organic substances, etc. other than the above minerals may be included in the extraction process. “Mineral” may be used interchangeably with “mineral complex”.

In addition, in the present invention, "mineral" refers to a mineral extracted from lichens by a conventional method or separated therefrom. In addition, in the present invention, "mineral" is used to include not only the extract, but also its dry powder or all forms formulated using the same.

The extract refers to a solvent in which the active ingredient contained in the extraction material is transferred by contacting the solvent and the extraction material under specific conditions. can be used regardless.

In addition, the dry powder may be produced through a process of desalting and filtering and drying from the extract. The desalting and filtration process is a process of removing suspended solid particles from the extract, and may be used to filter particles using two sides, nylon, etc., or to use ultrafiltration, freezing filtration, centrifugal separation, etc., but is not limited thereto. In addition, a separation process by various chromatography (chromatography according to size, charge, hydrophobicity or affinity) may be further included. In addition, the drying step includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, reduced pressure drying, foam drying, high frequency drying, infrared drying, and the like. In some cases, a process of pulverizing the final dried extract may be added.

The mineral of the present invention refers to a material produced through the extraction process, desalination and filtration process, and drying process, and the calcium (Ca), phosphorus (P), potassium (K), iron (Fe), zinc (Zn), In addition to those referred to as general minerals such as copper (Cu), manganese (Mn), and selenium (Se), vitamins, organic substances, etc. other than the above minerals may be included in the extraction process.

The mineral of the present invention is characterized in that it has a biological composition and a salt concentration similar to that of the extracellular matrix component. The extracellular matrix is composed of biopolymers filling the tissue or extracellular space and polymers and minerals supplied from body fluid, which exist in large amounts in connective tissue, are synthesized by cells, and are secreted or accumulated outside the cells. The main component is composed of fibrous proteins such as collagen and elastin, complex proteins such as proteoglycans and glycosaminoglycans, and cytotoxic glycoproteins such as fibronectin and laminin. The mineral has an effect of alleviating ion imbalance by supplying minerals to the skin while maintaining the osmotic pressure of skin cells due to the characteristic having a salt concentration similar to that of the extracellular matrix component.

On the other hand, the cosmetic composition according to an embodiment of the present invention may include a complex fermented Galactomyces.

The fermented Galactomyces complex is a natural material containing a large amount of organic substances and can be obtained by fermenting lichen-derived minerals with a Galactomyces strain.

As the yeast of the genus Galactomyces, the fermented Galactomyces promotes the conversion of profilagrin, a precursor of natural moisturizing factor (NMF), into a natural moisturizing factor to moisturize the skin. can be improved In addition, the rich polysaccharide component contained in Galactomyces prevents moisture evaporation within the skin surface, and is rich in various vitamins such as vitamins B1, B2, pantothenic acid, B6, and folic acid. Various natural enzymes such as proteases increase the production and absorption of active substances, and thus have antioxidant, anti-aging, whitening, and skin moisturizing effects.

In the method for manufacturing complex fermentation using Galactomyces and lichen-derived minerals of the present invention, after adding lichen-derived minerals to the Galactomyces culture, the secondary cultured material is crushed and filtered. .

The complex fermented material using the galactomyces and lichen-derived minerals is composed of a biological composition similar to the extracellular matrix component, and thus has the effect of improving the skin barrier and alleviating ion imbalance.

In addition, by adding a separate inoculum other than Galactomyces and combining the metabolites of the separate inoculum, a wide range of skin improvement effects can be obtained.

In another aspect, the present invention relates to a functional cosmetic for alleviating ion imbalance containing a lichen-derived mineral and a Galactomyces complex fermented product.

The functional cosmetics of the present invention can be prepared in the form of general emulsified formulations and solubilized formulations. Cosmetics with emulsified formulations include nutritional lotions, creams, and essences, and solubilized cosmetics include softened lotions.

Formulations of cosmetics suitable for the present invention include, for example, solutions, gels, solid or kneaded dry products, emulsions obtained by dispersing an oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes), nonionic It may be provided in the form of a vesicular dispersant in the form of a cream, skin, lotion, powder, ointment, spray, or in the form of a cone stick. In addition, it may be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

The cosmetic of the present invention further comprises fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, film formers, water, ions Type or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any of the conventionally used in cosmetics. It may contain adjuvants commonly used in the field of cosmetology or dermatology, such as other ingredients. And, the above ingredients may be introduced in an amount generally used in the field of dermatology.

The functional cosmetic of the present invention has the effect of improving the skin barrier and alleviating ion imbalance, and furthermore, since it has excellent antioxidant and whitening effects, it can be usefully used as a functional cosmetic for preventing skin aging.

Hereinafter, the content of the present invention will be described in more detail with reference to the following examples. However, it should be noted that the scope of protection of the present invention is not limited by the following examples.

1. Preparation of lichen-derived minerals and fermented Galactomyces complex

<Example 1> Lichen collection and identification

Eight species of lichens (Cetraria aculeate, Cladoniafurcata, Pseudephebe pubescens, Sphaerophorus globosus, Stereocaulon alpinum, Umbilicaria antarctica, Usnea antarctica and Ucoatsnea aurantia) collected from the southwest of King George Island in the South Shetland Islands of Antarctica Lichens) and 9 types of lichens (hereinafter referred to as Chinese lichens for convenience) and Usnea longissima (hereinafter referred to as lichens from Jirisan Mountain for convenience) collected from the alpine region were collected. Eight species of Antarctic lichens were identified by the method described by Kim et al. (2006) (Kim et al, Lichen Flora around Korean Antarctic Scientific Station, King George Island, Antartic. JMicrobiol 44:480-491 (2006)), and Suncheon University 9 kinds of Chinese lichens were identified by the Kunming Institute of Botany of the Chinese Academy of Science (CAS) and stored in CAS, China, and their copies were stored in Suncheon. It was also kept in the University's Korean Lithium Research Center.

<Example 2> Lichen-derived mineral extraction

Lichens according to Preparation Examples 1 to 5 are separated, washed with purified water, dried at room temperature so that the moisture content is about 10% or less, and pulverized to a size of 350 μm to 2,000 μm through a stainless steel mixer, and the pulverized product is crushed to 0.05 to It was dispersed in purified water at a concentration of 0.5 wt%. Thereafter, extraction was performed with stirring at 5 to 30 rpm at 60 to 120° C. for 1 to 6 hours, desalted in an ultrafiltration device (Pall, SLP-3053), and freeze-dried to obtain a lichen-derived mineral complex.

<Preparation Example 1> Preparation of mineral complex derived from Antarctic lichen

A mineral complex was prepared from the lichen Umbilicaria antarctica species according to the method of Example 2.

<Preparation Example 2> Manufacture of Chinese lichen-derived mineral complex

A mineral complex was prepared from the lichen Usnea barbata species according to the method of Example 2.

<Preparation Example 3> Preparation of mineral complex derived from lichens from Jirisan

A mineral complex was prepared from the lichen Usnea longissima species according to the method of Example 2.

<Preparation Example 4> Manufacture of mineral complexes of Antarctic lichen and Chinese lichen

A mineral complex was prepared according to the method of Example 2 using the lichen Umbilicaria antarctica species and the lichen lichen Usnea barbata species in a 1:1 ratio.

<Preparation Example 5> Preparation of mineral complexes of Antarctic lichens and Jirisan lichens

A mineral complex was prepared according to the method of Example 2 using the lichen Umbilicaria antarctica species and the lichen Usnea longissima species in a 1:1 ratio.

<Preparation Example 6> Manufacture of mineral composites of Chinese lichens and lichens from Jiri

A mineral complex was prepared according to the method of Example 2 by using the lichen Usnea barbata species and the lichen Usnea longissima species in a 1:1 ratio.

<Example 3> Preparation of mineral galactomyces complex fermented product

Fermentation medium containing glucose, sugar, starch syrup and yeast (glucose 1.5% (v/v), sugar 1.5% (v/v), starch syrup 1.5% (v/v), yeast extract 0.3% (v/v) v)) was inoculated with 5 to 10 parts by weight of the dried Galactomyces strain compared to yeast, and then pre-cultivated at 35° C. for 24 to 36 hours.

0.01 to 0.5 parts by weight of the lyophilized lichen-derived minerals of Preparation Examples 1 to 6 were added to a medium having the same composition as the fermentation medium, and then further cultured at 35° C. for 12 to 24 hours, at this time Galactomyces (Galactomyces) Glucose and sugar contents were controlled to improve the growth rate during culture.

Then, after crushing to a predetermined size, centrifugation and filtration were performed to remove the strain, and a mineral Galactomyces complex fermented material was obtained.

<Comparative Example> Preparation of Galactomyces Complex Fermented Product

Fermentation medium containing glucose, sugar, starch syrup and yeast (glucose 1.5% (v/v), sugar 1.5% (v/v), starch syrup 1.5% (v/v), yeast extract 0.3% (v/v) v)) was inoculated with 5 to 10 parts by weight of the dried Galactomyces strain compared to yeast, and then cultured at 35° C. for 24 to 36 hours. At this time, in order to improve the growth rate during culturing Galactomyces, glucose and Sugar content was controlled.

Then, after crushing to a certain size, centrifugation and filtration were performed to remove the strain, and a Galactomyces complex fermented material was obtained.

2. Galactomyces complex fermented mineral analysis containing lichen-derived minerals

The mineral analysis of the Galactomyces complex fermented product cultured with the addition of the lichen-derived minerals of Preparation Examples 1 to 6 and the Galactomyces complex fermented product cultured without the addition of the lichen-derived mineral prepared in Comparative Examples is inductively coupled plasma mass spectrometry mass Mineral components and content were analyzed using an analyzer (ICP-MS, X-series (X5, X7), Thermo Elemental, UK), and the analysis results are shown in Table 1 below.

division	Ca	P	K	Fe	Zn	Cu	Mn	Se	Mg
Preparation Example 1	269	27	11	ND	ND	ND	9	ND	13
Preparation 2	447	52	25	29	26	15	21	3	21
Preparation 3	432	40	13	17	18	26	12	4	17
Preparation 4	386	38	15	10	9	8	14	3	15
Preparation 5	347	42	27	32	22	14	23	8	19
Preparation 6	570	77	48	20	36	84	52	12	34
comparative example	15	5	ND	ND	ND	3	ND	ND	ND

In Table 1, the unit of all components is “ppb”.

3. Preparation of standard cosmetic samples containing Galactomyces complex fermentation

Using the Galactomyces complex fermented product prepared in Examples and Comparative Examples, cosmetic standard samples were prepared as shown in Table 2 below.

Main Phase	Raw material name	control	Formulation Example 1	Formulation Example 2	Formulation Example 3	Formulation Example 4	Formulation Example 5	Formulation Example 6
A	Purified water	56.18	56.18	56.18	56.18	56.18	56.18	56.18
	Galactomyces Complex Fermentation Filtrate	10.00	10.00	10.00	10.00	10.00	10.00	10.00
	Glycerine	5.00	5.00	5.00	5.00	5.00	5.00	5.00
	propanediol	5.00	5.00	5.00	5.00	5.00	5.00	5.00
	Betain	5.00	5.00	5.00	5.00	5.00	5.00	5.00
	Dipropylene Glycol	3.00	3.00	3.00	3.00	3.00	3.00	3.00
	1,2-Hexanediol	0.80	0.80	0.80	0.80	0.80	0.80	0.80
	Disodium EDTA	0.02	0.02	0.02	0.02	0.02	0.02	0.02
B	Xanthan Gum	0.05	0.05	0.05	0.05	0.05	0.05	0.05
	Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer	0.40	0.40	0.40	0.40	0.40	0.40	0.40
	Polyacrylate Crosspolymer-6	0.40	0.40	0.40	0.40	0.40	0.40	0.40
C	Polyglyceryl-3 Methylglucose Distearate	2.00	2.00	2.00	2.00	2.00	2.00	2.00
	Cetearyl Alcohol	3.00	3.00	3.00	3.00	3.00	3.00	3.00
	Butyropermum Parkii (Shea) Butter	1.00	1.00	1.00	1.00	1.00	1.00	1.00
	Caprylic/Capric Triglycerides	3.00	3.00	3.00	3.00	3.00	3.00	3.00
D	Methyl Trimethicone	3.00	3.00	3.00	3.00	3.00	3.00	3.00
	Dimethicone	2.00	2.00	2.00	2.00	2.00	2.00	2.00
E	Fragrance	0.15	0.15	0.15	0.15	0.15	0.15	0.15

In Table 2, the units of the components are all g units, Formulation Examples 1 to 6 are galactomyces complex fermented products containing lichen-derived minerals prepared in Examples 1 to 6, and Formulation Examples 1 to 6 are in Preparation Examples 1 to 6 above. Each prepared lichen-derived mineral was used. In contrast, the control group was prepared by using the Galactomyces complex fermented product not containing the lichen-derived minerals prepared in Comparative Example.

4. Cytotoxicity test

Human Dermal fibroblasts (HDFa) mixed with Medium 106 and Low Serum Growth Supplement in a medium containing penicillin (100IU/ml) and streptomycin (100μg/ml) at 37°C in an incubator containing 5% CO2 cultured within. In order to confirm the toxicity of the prepared cosmetic composition, cells were dispensed in a 96 well plate and then cultured for 18 hours in cell culture conditions. After removing the medium and washing with PBS, a new medium and a cosmetic composition were added at 0.1, 0.5, 1, 2, and 5% concentrations, respectively, and incubated for 18 hours. After 18 hours, in order to measure the viability of the cells, the mitochondria activity of the living cells was measured to confirm the cell viability by performing the MTT ASSAY to check the viability of the cells, and the results are shown in Table 3 below.

division	Cell viability (%)
Formulation Example 1	104.6
Formulation Example 2	109.3
Formulation Example 3	107.2
Formulation Example 4	107.5
Formulation Example 5	106.2
Formulation Example 6	112.4
control	93.8

As shown in Table 3, the cosmetic compositions of Formulation Examples 1 to 6 of the present invention showed a high cell viability of 100% or more for fibroblasts compared to the cosmetic composition according to the control.

5. Moisturizing power measurement

In order to measure the moisturizing power of the cosmetic prepared from the Galactomyces complex fermented product cultured by adding the lichen-derived mineral of the present invention, the moisturizing power was confirmed for Preparation Examples 1 to 6 and the control group of the cosmetic standard samples prepared in Table 4 above. This was measured using a Corneometer® CM825 (Courage and Khazaka electronic GmbH, Germany).

Before using the cosmetic standard sample, the skin moisture content was measured, and after use, the skin moisture content over time was re-measured. In addition, the moisture content increase rate of the skin moisture content and the moisture content before use was measured when 10 minutes elapsed after using the cosmetic standard sample, and the measurement results are shown in Table 4 below.

division	Skin moisture content over time (AU)						Increase rate of skin moisture content ((After use - Before use)
	before use	10 minutes	20 minutes	30 minutes	60 minutes	120 minutes	120 minutes
Preparation Example 1	29.8	37.1	41.8	42.5	44.1	45.6	53.0%
Preparation 2	27.8	38.2	42.7	43.4	45.8	45.9	65.1%
Preparation 3	26.5	33.8	39.5	40.7	41.8	42.7	61.1%
Preparation 4	27.2	36.3	39.8	42.1	44.2	43.1	58.5%
Preparation 5	26.2	30.7	36.2	38.7	39.7	40.9	56.1%
Preparation 6	28.3	38.6	43.2	46.1	47.2	48.4	71.0%
control	28.5	33.9	36.5	38.2	38.9	39.4	38.2%

As a result of measuring the moisturizing power by the above method, it was found that the moisture content of the skin coated with the Galactomyces complex fermented material cultured with lichen-derived minerals was higher than that of the Galactomyces fermented material cultured without containing the lichen-derived minerals.

6. Osmotic pressure balance effect

In order to measure the osmotic pressure balance effect of a cosmetic prepared from a Galactomyces complex fermented product cultured by adding a lichen-derived mineral of the present invention, IL- showing changes during hyperosmotic strees and hypoosmotic stress 6 and IL-33 expression inhibition experiments were performed. Specifically, human fibroblasts (Human Dermal fibroblast: HDFa) ^{were inoculated in a Petri dish at a density of 104/cm 2} and cultured for 24 hours, including 200 mmol/L of NaCl for IL-6 expression due to hypertonic stress. was replaced with a hypertonic solution stress medium and cultured for 24 hours. At this time, in order to confirm the hypertonic solution stress inhibitory effect of the Galactomyces complex fermented product, 5% v/v of the Galactomyces complex fermented product was added to the hypertonic solution medium and cultured for additional 24 hours. After culturing, cells were obtained by the following method. Cells were lysed on ice for 1 hour with a lysis buffer containing IGEPAL-Nonidet 1% (Sigma) and 1% protease inhibitor complex (Sigma) in PBS solution, then the cells were obtained with a scraper, followed by the Bradford method to measure the total protein content. Thereafter, the expression level of IL-6 is measured for the obtained solution through ELISA analysis. For the stock solution stress experiment, the stock solution stress medium using a calcium-free medium was replaced with the hypertonic solution stress medium in the above experimental method and performed in the same manner.

(1) Reduction of IL-6 generated during hyper-osmetic stress

The results are shown in Table 5 and FIG. 1 .

sample		IL-6 expression level (pg/mL)
contrast example	Hypertonic solution stress(-) / normal medium	82 ± 6
comparative example	Hypertonic solution stress(+) / normal medium	194 ± 10
Preparation 6	Hypertonic solution stress(+) / Galactomyces complex fermentation mixture medium	103 ± 7

The cosmetic standard sample prepared from the Galactomyces complex fermented product with the addition of lichen-derived minerals of the present invention compared to the cosmetic standard sample prepared from the Galactomyces fermented product cultured without the lichen-derived mineral was subjected to hypertonic solution stress ( It can be seen that the reduction range of IL-6 generated during hyper-osmetic stress) is large, confirming that it is advantageous in regulating the balance of hypertonic solution osmotic pressure.

(2) Reduction of IL-33 generated during hypo-osmetic stress

The results are shown in Table 6 and FIG. 2 .

sample		IL-33 expression level (pg/mL)
contrast example	Stock Stress(-) / Normal Medium	71 ± 4
comparative example	Stock Stress(+) / Normal Medium	148 ± 21
Preparation 6	Stock Stress(+) / Galactomyces Complex Fermentation Mixed Medium	92 ± 14

The cosmetic standard sample prepared from the Galactomyces complex fermented product with the addition of lichen-derived minerals of the present invention compared with the stock solution stress ( Hypo-osmetic stress), the reduction range of IL-33 generated is large, so it can be confirmed that it is advantageous to control the osmotic pressure balance of the stock solution.

7. Skin barrier improvement effect

In order to measure the skin barrier improvement effect of the standard cosmetic sample prepared from the Galactomyces complex fermented product cultured by adding the lichen-derived mineral of the present invention, TEWameter TM 300 (Courage & Khazaka, Cologne, Germany) was used for transdermal moisture loss. (TEWL, transepidermal water loss) was measured.

The results are shown in Table 7 and FIG. 3 .

sample		TEWL (% of control)
contrast example	Tape Stripping(-) / No Treatment	100 ± 6
comparative example	Tape Stripping (+) / Control	251 ± 16
Preparation 6	Tape Stripping(+) / Galactomyces Complex Fermentation Mixed Standard Sample	143 ± 9

The cosmetic standard sample prepared from the Galactomyces complex fermented product grown by adding the lichen-derived mineral of the present invention has a lower TEWL level than the cosmetic standard sample prepared from the Galactomyces fermented product cultured without the lichen-derived mineral. It can be seen that the degree of damage to the epidermal permeation barrier due to damage to the stratum corneum is reduced, thereby confirming the effect of improving the skin barrier.

8. Skin elasticity improvement effect

In order to measure the skin elasticity improvement effect of the standard cosmetic sample prepared from the Galactomyces complex fermented product grown by adding the lichen-derived mineral of the present invention, a skin elasticity measuring instrument (Cutometer MPA580, Conrage + Khazaka, Germany) was used. Skin elasticity was measured.

The measurement unit value is an arbitrarily set unit of the measuring device. The R value indicates the firmness of the skin, and the value decreases as the elasticity of the skin is good. The F value indicates the elasticity of the skin and the measurement value is close to 1. The higher the value, the higher the elasticity, the Q value indicates the degree of tiredness of the skin, and the smaller the value, the less the guideline, and the measurement results are as shown in Table 8 below.

division	R(mean)	F(mean)	Q(mean)
Preparation Example 1	0.299	0.039	0.351
Preparation 2	0.284	0.043	0.343
Preparation 3	0.338	0.026	0.404
Preparation 4	0.321	0.029	0.397
Preparation 5	0.317	0.036	0.375
Preparation 6	0.279	0.047	0.331
control	0.384	0.021	0.462

The cosmetic standard sample prepared from the Galactomyces complex fermented product grown by adding the lichen-derived mineral of the present invention has a smaller R value than the cosmetic standard sample prepared from the Galactomyces fermented product cultured without the lichen-derived mineral. It can be seen that the skin is firm and has good elasticity, the F value is close to 1, the skin elasticity is excellent, and the small Q value indicates a low level of tiredness of the skin. Features, structures, effects, etc. can be combined or modified for other embodiments by those of ordinary skill in the art to which the embodiments belong. Accordingly, the contents related to such combinations and modifications should be interpreted as being included in the scope of the present invention.

Claims (4)

1. Cosmetic composition comprising a complex fermented product prepared by mixing and culturing lichen-derived minerals and galactomyces
2. The method according to claim 1, wherein the lichen-derived minerals are Umbilicaria antarctica, Peltigera canina, Peltigera praetextata, Lamarina conduprikans (Ramalina). , conduplicans), Ramalina intermedia, Xanthoparmelia farinose, Diplocia canescens, Cetraria aculeate, Cladonia furcata ), Pseudephebe pubescens, Sphaerophorus globosus, Stereocaulon alpinum, Umbilicaria antarctica, Usnea antartica (Usnea antarctica), Usnea longissima (Usnea longissima), Usnea barbata (Usnea barbata) and Usnea aurantiacoatra (Usnea aurantiacotra) or any one selected from the group comprising Stictanylanderiana A cosmetic composition characterized in that it is a mineral derived from one or more lichens.
3. According to claim 1, wherein the lichen-derived minerals include Na, Fe, K, Mg, Al, Mn, Si, Ca, Zn, I, S, P, Se, Mg or pharmaceutically or cosmetically acceptable minerals thereof. A cosmetic composition comprising one or more minerals selected from the group comprising
4. The cosmetic composition according to claim 1, wherein the cosmetic composition has an effect of improving the skin barrier and alleviating skin cell ion imbalance.

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