KR102075103B1 - Cosmetic composition containing the fermented extract of okamejei kenojei - Google Patents

Abstract

The present invention provides a cosmetic composition containing a fermented extract of Okamejei kenojei or a nose thereof as an active ingredient. The fermented extract of Okamejei kenojei or a nose thereof is obtained by sterilizing a hydrolyzate obtained by hydrolyzing Okamejei kenojei or a nose of Okamejei kenojei with an enzyme, followed by fermenting with a fermentation strain, and suppress the production of collagen degrading enzyme MMP-1, thereby reducing skin wrinkles, and increase the expression of aquaporin-3 (AQP-3), thereby improving skin moisturization. In addition, the cosmetic composition according to the present invention contains a natural extract as an active ingredient, and thus has fewer side effects and is safer.

Description

Cosmetic composition containing a skate fermentation extract as an active ingredient {COSMETIC COMPOSITION CONTAINING THE FERMENTED EXTRACT OF OKAMEJEI KENOJEI}

The present invention relates to a cosmetic composition containing the skate fermentation extract as an active ingredient.

Skin is an important organ responsible for various physiological functions such as barrier function, temperature control, and excretion that protect the human body from the external environment and prevent internal moisture and useful components from leaking out. In general, however, as the skin ages, the skin loses its moisture, elasticity, color, and gloss due to internal and external environments. This process is called skin aging. Skin aging can be classified into intrinsic aging, which is an aging phenomenon with the passage of time, and photo aging, which is an aging phenomenon due to long exposure to sunlight. Both endogenous aging and photoaging are identical in that they are caused by harmful free radicals, but photoaging is different in that these free radicals are generated by ultraviolet light.

Skin aging can be promoted by matrix metalloproteinase-1 (hereinafter referred to as MMP-1), a collagen degrading enzyme. In vivo, the synthesis and degradation of extracellular matrix such as collagen is properly regulated, but as aging progresses, collagen synthesis decreases and the expression of collagen degrading enzyme MMP-1 is promoted, resulting in decreased skin elasticity and wrinkles. . Therefore, in order to prevent skin aging and wrinkles, it is necessary to regulate or inhibit the expression of MMP-1 in which activation is induced in cells.

The most effective way to improve wrinkles known to date is to use retinol and retinoic acid. Wrinkle improvement or elasticity improvement effect of retinol and retinoic acid is well known in the literature, but has a disadvantage of irritating the skin with only a small amount, there are many restrictions to use because it is easily oxidatively deteriorated when exposed to air due to instability. Research into stabilizing retinoids is ongoing, but the safety issue for the skin has not been solved.

On the other hand, ultraviolet light is known to reduce the expression of aquaporin-3 (hereinafter referred to as AQP-3) to cause skin dryness. AQPs are small hydrophobic integral membrane proteins that are responsible for the specific transport of moisture. Human keratinocytes express AQP-3 in their cell membranes, and when AQP-3 deficiency occurs, skin problems such as skin moisturization, skin barrier damage, and wound healing decrease.

Korea Registered Patent No. 10-1230650 (registered on Jan. 31, 2013)

In order to solve the above problems, the present invention provides a cosmetic composition containing the skate fermentation extract as an active ingredient.

The cosmetic composition according to the present invention may contain a skate oil fermentation extract or a skate oil fermentation extract as an active ingredient.

The cosmetic composition comprising the skate oil fermentation extract or skate oil fermentation extract according to the present invention as an active ingredient can be used as a functional cosmetics having an excellent effect. The skate oil fermentation extract or skate oil fermentation extract inhibits the production of collagen degrading enzyme MMP-1, can improve skin wrinkles, increase the expression of aquaporin-3 (AQP-3), improve skin moisture You can. In addition, since the cosmetic composition according to the present invention contains a natural extract as an active ingredient, it has little side effects and is safe, and can resolve consumer's rejection of various cosmetic products containing synthetic chemicals that irritate the skin.

Figure 1 is a graph of the molecular weight of the Hong Sikko fermented extract according to the present invention using high performance liquid chromatography-gel permeation chromatography (HPLC-GPC).
Figure 2 is a graph measuring the inhibitory ability of collagen degrading enzyme MMP-1 production according to the concentration of the skates extract according to an embodiment of the present invention.
Figure 3 is a graph measuring the inhibitory ability of MMP-1 production according to the concentration of the skate oil extract according to an embodiment of the present invention.
Figure 4 is a graph measuring the inhibitory ability of MMP-1 production according to the concentration of the skate fermentation extract according to an embodiment of the present invention.
Figure 5 is a graph measuring the inhibitory ability of MMP-1 production according to the concentration of the skate oil fermentation extract according to an embodiment of the present invention.
Figure 6 is a graph measuring the effect of aquaporin-3 (AQP-3) expression according to the concentration of the skates according to an embodiment of the present invention.
Figure 7 is a graph measuring the effect of AQP-3 expression according to the concentration of the skate oil extract according to an embodiment of the present invention.
Figure 8 is a graph measuring the effect of AQP-3 expression according to the concentration of the skate fermentation extract according to an embodiment of the present invention.
Figure 9 is a graph measuring the effect of AQP-3 expression according to the concentration of the skate oil fermentation extract according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The present inventors focused on functional cosmetics containing natural extracts, and researched the applicability to skin wrinkle improvement or moisturizing cosmetic compositions in various raw materials, and completed the present invention by selecting skate as a natural product.

The present invention provides a cosmetic composition containing a skate oil fermentation extract or skate oil fermentation extract as an active ingredient.

In the present invention, "skate (Okamejei kenojei)" is a cartilage, benthic fish belonging to the stingray family is distributed in the southwest sea of Korea, the south central Japan and the East China Sea. Skates have high commercial value in Korea, and they are used as traditional fermented foods in Korea. Skates contain unsaturated fatty acids to lower blood cholesterol levels, and taurine and betaines help to relieve hangovers and improve liver function, and are rich in Chondroitin sulfate, which is used to treat arthritis. It is also effective in improving bone health, including prevention of osteoporosis. In addition, skates contain a variety of nutrients that are good for the skin, along with collagen, it can be used to create elasticity and reduce wrinkles on the skin. In particular, the skates in the triangle-shaped area in front of the skates contain a large amount of chondroitin sulfate, proteoglycans and the like.

The cosmetic composition according to the present invention may contain a skate oil fermentation extract or a skate oil fermentation extract, and the skate oil fermentation extract may be a fermentation extract obtained from any one or more selected from the tail, tail, or body of skate.

In the cosmetic composition according to the present invention, the skate oil fermentation extract or skate oil fermentation extract may be a fermentation strain after sterilizing the hydrolyzate obtained by hydrolyzing the skate oil or skate oil with enzyme.

The hydrolyzate may include those obtained by primary hydrolysis of the skate or redfish nose with alcalase, and secondary hydrolysis with protease. The enzyme is a proteolytic enzyme, and can be hydrolyzed by addition of chymotrypsin, pepsin, etc. in addition to the alkalase and protease, but is not limited thereto. According to the type of the enzyme, it can be hydrolyzed by adjusting the temperature, pH, and reaction time.

In the cosmetic composition according to the invention, the hydrolyzate may comprise a proteoglycan.

In the present invention, "Proteoglycan" is a core protein (glycosaminoglycan) is combined with the core protein (core protein), it is contained in bone, cartilage, skin, ligaments and the like. Representative glycosaminoglycans include heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate, keratan sulfate, and hyaluronan. There are two or more of these glycosaminoglycans combined in combination, showing very complex molecular diversity.

The proteoglycan molecule has a lot of hydroxyl groups (-OH), and has excellent water-binding ability because it has excellent binding force with water. In addition, it protects the fiber and cellular components of the tissue, and helps to improve skin elasticity by having elasticity to withstand the pressure applied to the surface of the tissue. However, in general, proteoglycan is a polymer having a molecular weight of about 1.2 million Da, so the absorption rate is low on the skin.

In the cosmetic composition according to the present invention, the skate oil fermentation extract or skate oil fermentation extract may be the fermentation of the proteoglycan with the fermentation strain. If the proteoglycan polymer is low molecular weight through fermentation, it can be better absorbed by the skin.

The fermentation strain may include brewer's yeast ( Saccharomyces cerevisiae ), yeast ( Aspergillus oryzae ), Lactobacillus rhamnosus , Galactomyces reessii , black yeast ( Aureobasidium pullulans ), etc., preferably Lactobacillus rhamnosus.

In the present invention, " Lactobacillus rhamnosus " is a beneficial bacterium found in the human digestive system, the lining of the vagina, and the oral cavity of the human body, which helps to improve overall digestion and prevents the death of visceral cells by diarrhea and visceral ulcers. And so on. It has an antibiotic effect against Escherichia coli, Staphylococcus aureus, and can alleviate allergies and heal dermatitis, eczema, acne and atopy. In addition, it contains an extracellular polysaccharide (exopolysaccharide), a metabolite produced during the cultivation process, thereby increasing the skin's moisturizing ability and clearing the skin. In the present invention, Lactobacillus rhamnosus can be low molecular weight by decomposing the polysaccharide proteoglycan during the fermentation process.

In the cosmetic composition according to the present invention, the skate oil fermentation extract or skate oil fermentation extract after the fermentation, water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, It may be prepared by extraction with a solvent selected from the group consisting of chloroform, butyl acetate, diethyl ether, dichloromethane, hexane and mixtures thereof. In addition to the solvent extraction may be prepared by adding, extracting and purifying additional methods.

Not limited to the solvent extraction, the skate oil fermentation extract or skate oil fermentation extract is a variety of extraction and separation methods commonly used in the art, for example, extraction by ultra-high pressure extraction, constant molecular weight cut-off It may be prepared by an additional method such as separation using an ultrafiltration membrane having a value, and separation by chromatography prepared for separation according to size, charge, hydrophobicity or affinity.

The ultra-high pressure extraction method can be extracted by treating the skates or skates 20 to 30 hours at a pressure of 100 to 3000 MPa, temperature 40 to 90 ℃, preferably 23 to 25 hours at a pressure of 500 to 1000 MPa, temperature 40 to 60 ℃ have. In addition, the pH at the time of ultra-high pressure extraction is not limited to a specific pH, but it is more preferable to lower the pH of the water inside the ultra-high pressure to 1 to 6.

The ultra-high pressure skated fermented extract or skated fermented extract was refluxed and chilled using a solvent selected from water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, hexane, and mixtures thereof. Can be filtered. The filtrate may be transferred to a concentration tank and concentrated under reduced pressure and freeze-dried at 50 ° C or less.

The extract, reduced pressure concentrate or lyophilized product obtained according to the above method may be formulated in a liquid phase with one or more solvents selected from the group consisting of purified water, ethanol, butylene glycol, and propylene glycol. Without being limited thereto, the cosmetic composition may be prepared in any dosage form that is commonly prepared, and more specifically, may be prepared in the form of a lotion, essence, lotion, cream, pack, foundation, gel, ointment or spray. .

In the cosmetic composition according to the present invention, the skate oil fermentation extract or skate oil fermentation extract may be contained 0.001 to 20% by weight relative to the total weight of the cosmetic composition. When the fermentation extract is included in less than 0.001% by weight, the effect of exhibiting the various skin functionalities identified in the present invention is insignificant, and when it exceeds 20% by weight, the efficiency of the material input is not economically low.

Cosmetic compositions according to the present invention include auxiliaries commonly used in the cosmetic field, for example, hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, odorants, Dyes and the like. The amount of these various adjuvants is conventionally used in the art and may contain, for example, 0.001 to 20% by weight relative to the total weight of the composition. In addition, the cosmetic composition may include one or more other active ingredients exhibiting antioxidant, whitening, anti-wrinkle and anti-inflammatory effect in addition to the skate oil fermentation extract or skate oil fermentation extract. In any case, the adjuvant, the active ingredient and the content thereof will be selected so as not to adversely affect the desirable properties of the cosmetic composition according to the present invention.

In the cosmetic composition according to the present invention, the proteoglycan becomes low molecular weight through the fermentation process, thereby increasing the absorption rate in the skin. In addition, the cosmetic composition may have the effect of improving skin wrinkles or skin moisturizing. Through the following examples, the effect can be confirmed.

Hereinafter, examples will be described in detail to help understand the present invention. However, the following examples are merely to illustrate the content of the present invention is not limited to the scope of the present invention. The embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

[Example 1: Proteoglycan Yield and Content (%) According to Enzyme Treatment of Skate or Skate Nose Cartilage]

In this example, the yield of proteoglycans was determined after hydrolysis of each of the skaters or skateboard cartilage. Water equivalent to four times the cartilage volume of the chopped skates was added and hydrolysis was performed using five proteolytic enzymes for hydrolysis. By measuring the yield and proteoglycan of the hydrolyzate, the optimal enzyme concentration, pH, and reaction time for enzymatic hydrolysis were selected.

To select the appropriate proteolytic enzymes to increase the extraction efficiency of proteoglycans from skates, Flavorzyme, Protamex 1.5MG, Neutrase, Alcalase, and Protease Hydrolysis was carried out using Protease). Each of the five proteolytic enzymes was added in about 2.0%, and the pH and temperature of each enzyme were flavozyme pH 6.5, 45 ° C .; Protamex 1.5MG, pH 5.5, 40 ° C; Neutrase, pH 6.5, 40 ° C .; Alkalase, pH 7.0, 50 ° C .; Protease, adjusted to pH 7, 37 ° C., was hydrolyzed for 24 hours, respectively, and the proteoglycan yield and content of each digest was measured.

enzyme yield content Alcalase 19.6 17.1 Protease 20.5 17.8 Flavorzyme 11.2 8.8 Neutrase 13.1 6.9 Protamex 1.5MG 15.3 9.1

Table 1 shows the yield and content of proteoglycans according to proteolytic enzymes according to Example 1. Referring to Table 1, when hydrolysis was performed using alkalase and protease, the yield and content of the proteoglycan were the highest.

Example 2 Low Molecular Weight of Proteoglycans during Fermentation

In this embodiment, after the fermentation of the skates or skateboard cartilage for each fermentation strain, the low molecular weight of the proteoglycan was to be confirmed. Four times the volume of cartilage of chopped skates was added, followed by hydrolysis using alkalase and protease, which had high yield and content of proteoglycans in Example 1, followed by fermentation strains of beer yeast, koji, lactose. Bacillus rhamnosus. Galactomyces, fermented with black yeast.

Saccharomyces cerevisiae YM medium, 30 ° C .; Aspergillus oryzae PDA medium, 26 ° C., Lactobacillus rhamnosus MRS medium, 37 ° C .; Galactomyces reessii 4% Malt medium, 21 ° C. Black yeast ( Aureobasidium pullulans ) PDA medium, adjusted to 26 ℃ fermented for 24 hours, respectively, the proteoglycan molecular weight of each fermentation was measured.

Proteoglycan Brewer's yeast Yeast Lactobacillus rhamnosus Galactomyces Black yeast Molecular Weight (Da) 1,200,000 No change No change 5,900 No change No change

Table 2 measures the molecular weight of the proteoglycans of hydrolysates fermented using each fermentation strain according to Example 2. Referring to Table 2, when 1.2 million Da of proteoglycans were fermented by Lactobacillus rhamnosus, the molecular weight was about 1/200 of the initial molecular weight. In comparison, there was no change in molecular weight in the remaining fermentation strains.

Example 3: Preparation of Skate Extract

Skates were purchased, washed with water to remove impurities, and cartilage was collected from the trunk and tail of the skates using a knife as a starting material. Solid fat was removed from the cartilage of the skate using tweezers and washed with sterile saline. Subsequently, finely pulverized with a grinder to obtain cartilage of chopped skates. Hydrolysis was performed by adding water corresponding to four times the volume of cartilage and sequentially adding two enzymes.

First, the first hydrolysis was performed by adding an alkalase enzyme at 50 ° C. and pH 7 to react for 1 hour. At this time, alkalase to skate was mixed in a ratio of 1: 100 (W / W). After performing the first hydrolysis, a second hydrolysis was performed in which the protease, another enzyme, was added to the reaction product at 37 ° C. and pH 7 to react for 4 hours. At this time, the protease to skate was reacted by mixing at a ratio of 1: 100 (W / W).

After the second hydrolysis reaction was completed, the enzyme was inactivated by heating in a hot water bath for 10 minutes, and the hydrolyzate was separated. The hydrolyzate was filtered through whatman # 5 filter paper to prepare a skate extract.

Example 4 Preparation of Skate Nose Extract

Skates were purchased, washed with water to remove impurities, cartilage was collected from skates using a knife as a starting material. Solid fat was removed using tweezers from the cartilage of the skate, and washed with sterile saline. Subsequently, finely pulverized with a grinder to obtain finely sliced red nose nose cartilage. Hydrolysis was performed by adding water corresponding to four times the volume of cartilage and sequentially adding two enzymes.

First, the first hydrolysis was performed by adding an alkalase enzyme at 50 ° C. and pH 7 to react for 1 hour. At this time, alkalase to red nose was mixed and reacted at a ratio of 1: 100 (W / W). After performing the first hydrolysis, a second hydrolysis was performed in which the protease, another enzyme, was added to the reaction product at 37 ° C. and pH 7 to react for 4 hours. At this time, the protease to red nose was mixed and reacted at a ratio of 1: 100 (W / W).

After the second hydrolysis reaction was completed, the enzyme was inactivated by heating in a hot water bath for 10 minutes, and the hydrolyzate was separated. The hydrolyzate was filtered through a Whatman # 5 filter paper to prepare a skate oil extract.

Example 5 Preparation of Skate Fermented Extract

The skate extract prepared according to Example 3 was autoclaved at 121 ° C. and 50,000 cfu / L of Lactobacillus rhamnosus mycelium was added at 37 ° C., at pH 5 to 7, preferably at about 1 day in a fermenter at an aerobic condition of pH 6. The cultures were fermented for 7 days, preferably 3 days. After fermentation, the fermented product was sterilized at 121 ° C. and 1.5 atm for 15 minutes to kill bacteria, and then filtered using a 0.45 μm filtration membrane. The filtered fermented product was concentrated under reduced pressure at 50 ° C. or lower and freeze dried. The vacuum concentrated and freeze-dried products were prepared by concentration according to the following experimental example.

Example 6 Preparation of Safflower Fermented Extract

Safflower extract prepared according to Example 4 was autoclaved at 121 ° C. and 50,000 cfu / L of Lactobacillus rhamnosus mycelium was added thereto at 37 ° C., pH 5 to 7, preferably pH 6 for about 1 day in a fermentor. It was fermented by culturing for 7 days, preferably 3 days. After fermentation, the fermented product was sterilized at 121 ° C. and 1.5 atm for 15 minutes to kill bacteria, and then filtered using a 0.45 μm filtration membrane. The filtered fermented product was concentrated under reduced pressure at 50 ° C. or lower and freeze dried. The vacuum concentrated and freeze-dried products were prepared by concentration according to the following experimental example.

Dry Weight of Final Degradation (Unit: g) Skate Extract 61.5 Skate Nose Extract 60.7 Skate Ferment Extract 58.2 Safflower Ferment Extract 55.3

Table 3 shows the dry weights of the skates extracts, skates extract, skate fermentation extract, and the final digest of the skates fermentation extract prepared according to Examples 3-6.

In addition, Figure 1 is a graph of the molecular weight of the Sikko fermented extract according to the present invention using high performance liquid chromatography-gel permeation chromatography (HPLC-GPC). Referring to Figure 1, the Hakko fermented extract prepared according to Example 6 can be confirmed that the molecular weight is measured to a low molecular weight of 5,900 Da.

Experimental Example 1: Determination of MMP-1 Production Inhibition of Skate Fermented Extract or Skate Fermented Extract

In this experimental example, the inhibitory effect of the production of MMP-1, a collagen degrading enzyme of the skates, skates, skates fermented extracts, and skates fermented extracts obtained in Examples 3 to 6 was confirmed.

Human skin fibroblasts were aliquoted in a 24-well plate at a concentration of 2 × 10 ⁵ cells / well and incubated at 37 ° C., 5% CO ₂ for 24 hours. After washing twice with physiological saline (Phosphate buffer saline; hereinafter, PBS), UV was irradiated by 6 J / cm ² using a UV irradiator with physiological saline. The skate extract, skate skate extract, skate skate fermentation extract, and skate skate fermentation extract of Examples 3 to 6, DMEM (Dulbeco's Modified Eagle's Media) medium with 2% FBS (fetal bovine serum) and the extract and the fermentation extract This was incubated for 48 hours in 2% DMEM medium with no control.

After incubation, the supernatant of each well was collected and the amount (ng / ml) of newly synthesized MMP-1 was measured using an MMP-1 assay kit (R & D system, United States of America), and MMP-1 was generated according to Equation 1 below. Inhibition% was calculated.

[Equation 1]

2 to 5 are graphs of MMP-1 production inhibitory ability of collagen degrading enzymes according to the concentration of the skate, extract, skate, fermented extract, or skate, fermented extract according to an embodiment of the present invention.

Referring to Figures 2 and 3, it can be seen that the skate extract exhibits about 25% MMP-1 production inhibitory effect at 3% concentration, and the skate oil extract inhibits about 40% MMP-1 production at 3% concentration. As a result, the production inhibition of MMP-1 was higher than that of the skate.

Referring to FIGS. 4 and 5, the skate oil fermentation extract and the skate oil fermentation extract exhibited about 50% MMP-1 production inhibitory activity at a concentration of 3%, showing an effect similar to that of 100 μg / ml of adenosine. Can be confirmed.

That is, all of the skate extract, skate extract, skate fermentation extract and skate extract fermentation extract according to the present invention was found to have the ability to inhibit MMP-1 production, and among them, the excellent MMP-1 in the Shows the ability to inhibit production, it can be said that the fermented extract has better skin aging and anti-wrinkle effect than the extract.

Experimental Example 2: Confirmation of AQP-3 Expression Effect of Skate Fermented Extract or Skate Fermented Extract

In the present experimental example, it was intended to confirm the aquaporin-3 (AQP-3) expression effect of the skates, skates extract, skates fermented extract, and skates fermented extract obtained in Examples 3 to 6.

The skin keratinocytes (HaCaT) were dispensed and stabilized in 6 wells at 2/10 ⁵ cells / ml, and then stabilized. Cells were obtained using PBS, and then reacted for 30 minutes on ice using a cell lysis buffer to destroy cell membranes and centrifuged at 1,200 rpm for 15 minutes. After centrifugation, only the supernatant was transferred separately and the protein was quantified using BCA (Bicinchoninic acid) assay.

A certain amount of protein was mixed with SDS gel-loading buffer and heated at 100 ° C. for 3 minutes to inactivate it, followed by electrophoresis using SDS-polyacrylamide gel. PAGE). SDS-polyacrylamide gel was transferred to a polyvinylidene fluoride (PVDF) membrane at 60 mM for 90 minutes and blocked for 1 hour in 5% skim milk. The primary antibody (Aquaporin-3) was reacted with the blocked PVDF membrane at 4 ° C. for 24 hours, and washed with 30 × by replacing 3 × of the 1X TTBS solution at 10 minute intervals. The primary-specific secondary antibody in 5% skim milk was reacted for 2 hours, and washed again for 30 minutes by exchanging 3 times at 10 minute intervals with 1X TTBS solution. PVDF membranes were reacted with enhanced chemiluminescence (ECL) solution and bands were analyzed using a Chemi DocTM XRS + device.

6 to 9 are graphs measuring the effect of aquaporin-3 (AQP-3) expression according to the concentration of the skate, extract, skate, fermented, or skate fermentation extract according to an embodiment of the present invention .

6 to 9, it can be seen that the skate, extract, skate, fermented extract, skate, fermented extract, and skate, all of which increase the expression of AQP-3 in a concentration-dependent manner. In particular, the expression of AQP-3 was more clearly observed in the skate and fermented extracts, compared to the skate and the extract of skate, the skate and fermented extracts were the positive control dexamethasone at 3% concentration. Dexamethason) showed almost the same efficacy as 1μM.

In other words, through the present experiment, it was confirmed that all of the skate, extract, skate, fermented extract and skate, fermented extract according to the present invention increased the expression of AQP-3, and among them, skate and fermented extract In the expression of high AQP-3 is observed, it can be seen that the fermented extract exhibits a better skin moisturizing effect than the extract.

Preparation Example: Preparation of Cosmetics Containing Skate Fermented Extract and Skate Fermented Extract

Cosmetic compositions containing 3.0% by weight of the skate oil fermentation extract or skate oil fermentation extract according to Examples 5 and 6 were prepared in the composition of Table 4, Preparation Example 1 and Preparation Example 2. On the other hand, in order to confirm the efficacy cosmetic composition that does not include the skates fermented extract or skates fermented extract, that is, the cosmetic composition including the skates and skates extract as Comparative Preparation Example 1 and Comparative Preparation Example 2 in Table 4 Indicated.

division
ingredient Preparation Example 1
Content (% by weight) Preparation Example 2
Content (% by weight) Comparative Production Example 1
Content (% by weight) Comparative Production Example 2
Content (% by weight)

A Example 3 - - 3.0 - Example 4 - - - 3.0 Example 5 3.0 - - - Example 6 - 3.0 - - EDTA-2Na 0.02 0.02 0.02 0.02 Purified water to 100 to 100 to 100 to 100




B Cetostearyl alcohol 2.0 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 1.5 Microcrystallin 0.7 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 3.0 Trioctanoine 5.0 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 0.5 Tocopheryl Acetate 0.2 0.2 0.2 0.2 Cyclomethicone 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 C Incense, preservative Appropriate Appropriate Appropriate Appropriate

Experimental Example 3: Confirmation of Skin Wrinkle Improvement Effect of Cosmetic Composition Containing Skate Fermented Extract or Skate Fermented Extract

In this experimental example, the human body application test was performed on the skin wrinkle improvement effect of the cosmetic composition containing the skate oil fermentation extract or skate oil fermentation extract.

The human application test is a cosmetic composition containing the cosmetic composition of Preparation Example 1 or 2 containing Example 5 or 6, which showed the efficacy in Experimental Example 1, and Comparative Preparation Example 1 or 2 comprising a skate or extract The skin wrinkle improvement effect of the use of was confirmed and compared. The test was evaluated through the actual use test of each cosmetic.

Experimental (20-35 year old female) cosmetic composition comprising a skate fermentation extract or skate oil fermentation extract for 20 women, Preparation Examples 1 and 2, the control group of the experimental group to the right side of the face, Preparation Example 1 And 2 were applied to the left side of the face, respectively, to proceed with the experiment. Skin wrinkle improvement was evaluated by visual observation and instrumental measurement (cutometer SEM 575, C + K Electronic Co., Germany) of experienced doctors before and after 8 weeks of use. It is shown in Table 5 below.

Wrinkle improvement
% Improvement Great slightly none Preparation Example 1 (Example 5) 12 4 4 70.0 Preparation Example 2 (Example 6) 15 3 2 82.5 Comparative Production Example 1 (Example 3) 0 11 9 27.5 Comparative Production Example 2 (Example 4) 0 13 7 30.0

Table 5 shows the wrinkle-improving effect of the cosmetic composition comprising the skate oil fermentation extract or skate oil fermentation extract by visual evaluation and instrumental evaluation. Referring to Table 5, the cosmetic compositions of Preparation Examples 1 and 2 containing the skate oil fermentation extract and skate oil fermentation extract according to the present invention showed a high improvement rate for skin wrinkle improvement as compared to Comparative Preparation Examples 1 and 2 as a control group. . In particular, the cosmetic composition containing Preparation Example 2, Sikko fermented extract showed a very good skin wrinkle improvement effect. In addition, the experimental results indicate that the low-molecular fermentation extracts, such as the skates fermentation extracts, skates fermentation extracts are better absorbed into the skin than the macromolecular extracts, such as the skates, skates extracts, and more effective in improving wrinkles.

[Experimental Example 4: Confirming the skin moisturizing effect of the cosmetic composition containing the skate oil fermentation extract or skate oil fermentation extract]

In the present experimental example, it was intended to confirm the skin moisturizing improvement effect of the cosmetic composition containing the skates fermented extract or skates fermented extract prepared in the preparation example.

For 25 experimenters (20 ~ 40s) who do not have skin diseases, divided into 2 sets of 20 persons per set, and the cosmetic preparations of Preparation Examples 1 and 2 and Comparative Preparation Examples 1 and 2 were applied to the face and forearm. Application was made. Before the start of the application, the skin conductivity was measured by using a moisture meter (conneometer, CM820 courage Khazaka electronic GmbH, Germany) at constant temperature and humidity conditions (24 ° C., 40% humidity) in advance to evaluate the increase rate. The results are shown in Table 6 below.

Sample name 1 week later 2 weeks later 4 weeks later Preparation Example 1 48 52 55 Preparation Example 2 50 55 57 Comparative Production Example 1 26 30 35 Comparative Production Example 2 30 34 39

Table 6 shows the results of the skin moisturizing effect of the cosmetics containing the skates fermented extract or skates fermented extract according to Experimental Example 4. Referring to Table 6, Preparation Examples 1 and 2, which are the cosmetics containing the skates fermented extract or skates fermented extract according to the present invention, skin conductivity compared to the cosmetics containing the skates or skates extract of Comparative Preparation Examples 1 and 2 It can be seen that the increase rate is very excellent. In general, since the skin conductivity is proportional to the skin moisture content, the above results mean that the present invention cosmetic composition containing a skate fermentation extract or skate oil fermentation extract maintains a high skin moisture content to exert an excellent skin moisturizing effect .

So far, specific embodiments of the present invention have been described. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.

Claims (9)

As a cosmetic composition containing a skate fermentation extract or skate oil fermentation extract as an active ingredient,
The skate fermentation extract or skate oil fermentation extract is a cosmetic composition, characterized in that the proteoglycan obtained by hydrolyzing the skate or skate with an enzyme fermented with Lactobacillus rhamnosus ( Lactobacillus rhamnosus ). The method of claim 1,
The skates fermented extract,
Cosmetic composition, characterized in that the fermented extract obtained from any one or more selected from the tail, kelp, or body of the skate. delete delete The method of claim 1,
The proteoglycan,
Cosmetics, characterized in that obtained by the primary hydrolysis of the skate or the skate nose with alcalase, and secondary hydrolysis with protease (Protease). The method of claim 1,
The skate oil fermentation extract or skate oil fermentation extract,
Water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane and mixtures thereof Cosmetic composition, characterized in that prepared by extraction with a solvent. The method of claim 1,
The skate oil fermentation extract or skate oil fermentation extract,
Cosmetic composition, characterized in that contained 0.001 to 20% by weight relative to the total weight of the cosmetic composition. The method of claim 1,
The cosmetic composition,
Cosmetic composition, characterized in that for improving skin wrinkles. The method of claim 1,
The cosmetic composition,
Cosmetic composition for skin moisturizing.

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