KR102156898B1 - Ingestible cosmetic composition for skin whitening, skin moisturizing, antioxidant, anti-inflammatory, skin barrier strengthening and skin absorption containing the lactic acid fermentation mixture - Google Patents

Abstract

The present invention relates to a cosmetic composition. More particularly, the present invention provides a cosmetic composition which contains a lactobacillus fermentation mixture in the cosmetic composition, and the mixture is fermented with lactobacillus, but the mixture contains a Camellia sinensis seed oil, a Centella asiatica extract, astaxanthin, lecithin, a rice bran extract, ceramide 3, and dead sea bath salt, thereby being able to improve skin whitening, skin moisturizing, antioxidant, skin barrier strengthening, and skin absorption functions.

Description

Ingestible cosmetic composition for skin whitening, skin moisturizing, antioxidant, anti-inflammatory, skin barrier strengthening containing lactic acid bacteria fermentation mixture, skin whitening, skin moisturizing, antioxidant, anti-inflammatory, skin barrier strengthening and skin absorption function improvement and skin absorption containing the lactic acid fermentation mixture}

The present invention relates to a cosmetic composition, and more particularly, to an intakeable cosmetic composition containing a mixture of lactic acid bacteria fermentation and improving skin whitening, skin moisturizing, antioxidant, anti-inflammatory, skin barrier reinforcement and skin absorption function.

In general, human skin loses its elasticity and shine with aging, and becomes keratinized and becomes rough. Clinically, aging is characterized by wrinkle formation, reduction of skin elasticity, damage and death of skin cells, and systematically, loss of mature collagen and damaged elastin fibers accumulate over time. Such skin aging varies in progression speed and degree depending on several factors, such as genetic factors, exposure to ultraviolet rays, and generation of active oxygen due to physicochemical stress. Therefore, in order to maintain young and supple skin, efforts to supply externally, such as nutritional substances that can prevent the aging process, are generally made.

As the interest in skin beauty continues to increase, the cosmetics industry is expanding all over the world and various cosmetics are being developed, but since skin side effects from synthetic cosmetics are constantly being pointed out, cosmetic technology using natural ingredients that can minimize side effects There is interest in development.

In addition, toxic substances produced by harmful bacteria in the human body's stool enter the blood, causing toxicity in the face where blood vessels are relatively exposed, causing skin troubles. When lactic acid bacteria are ingested to eliminate these causes, there is a cosmetic effect on the skin by removing harmful bacteria and toxic substances present in the sukbyeon by removing sukbyeon.

The problem to be solved by the present invention is to provide an intakeable cosmetic composition containing a mixture of fermented lactic acid bacteria and improving skin whitening, skin moisturizing, antioxidant, anti-inflammatory, skin barrier enhancement and skin absorption.

The problems to be solved by the present invention are not limited to the problems mentioned above, and other problems that are not mentioned will be clearly understood by those skilled in the art from the following description.

In order to achieve the object of the present invention as described above, the cosmetic composition according to an embodiment of the present invention is green tea seed oil (Camellia Sinesis seed oil), centella asiatica extract, astaxanthin, lecithin ( Lecithin), rice bran extract (Oryza Sativa (rice) bran extract), ceramide 3 (Ceramide 3) and dead sea salt (sea salt) fermented with a lactic acid bacteria fermentation mixture.

In addition, the lactic acid bacteria may include Lactobacillus.

In addition, the green tea seed oil (Camellia Sinesis seed oil) is 0.1 to 50 parts by weight, the centella asiatica extract is 0.1 to 25 parts by weight, the astaxanthin (Astaxanthin) is 0.1 to 30 parts by weight, the lecithin (Lecithin) is 0.1 to 15 parts by weight, the rice bran extract (Oryza Sativa (rice) bran extract) is 0.1 to 30 parts by weight, the ceramide 3 is 0.1 to 25 parts by weight and the dead sea salt (sea salt) May be a mixture of 0.1 to 25 parts by weight.

In addition, the cosmetic composition may contain the lactic acid bacteria fermentation mixture in an amount of 0.001 to 90% by weight based on the total weight.

In addition, the cosmetic composition may include skin ointment, cream, softening lotion, nutritional lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, lotion , Balm, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, nutrition essence, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser It can be any one formulation.

The cosmetic composition containing the lactic acid bacteria fermentation mixture according to the present invention has the effect of improving skin whitening, skin moisturizing, antioxidant, anti-inflammatory, skin barrier strengthening and skin absorption.

Green tea seed oil, centella extract, astaxanthin, lecithin, rice bran extract, ceramide 3, and dead sea salt have effects such as skin whitening, skin moisturization, antioxidant, anti-inflammatory, skin barrier strengthening and skin absorption, so this is an active ingredient. The composition of the present invention including the composition can effectively improve the skin and can be usefully used as a cosmetic composition.

With reference to the accompanying drawings, skin whitening, skin moisturizing, antioxidant, anti-inflammatory, skin barrier reinforcement, and skin-absorbing function improved intakeable cosmetic composition containing the lactic acid bacteria fermentation mixture according to embodiments of the present invention will be described in detail. Since the present invention can apply various changes and have various forms, specific embodiments will be illustrated in the drawings and described in detail in the text. However, this is not intended to limit the present invention to a specific form of disclosure, and it should be understood that all changes, equivalents, and substitutes included in the spirit and scope of the present invention are included.

Further, terms such as first and second may be used to describe various components, but the components should not be limited by the terms. These terms are used only for the purpose of distinguishing one component from another component. For example, without departing from the scope of the present invention, a first element may be referred to as a second element, and similarly, a second element may be referred to as a first element. Meanwhile, unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms as defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and should not be interpreted as an ideal or excessively formal meaning unless explicitly defined in this application. Does not.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The present invention provides an intakeable cosmetic composition containing a mixture of lactic acid bacteria fermentation with improved skin whitening, skin moisturizing, antioxidant, anti-inflammatory, skin barrier enhancement and skin absorption. The mixture is green tea seed oil (Camellia Sinesis seed oil), Centella asiatica extract, Astaxanthin, Lecithin, Rice bran extract (Oryza Sativa (rice) bran extract), Ceramide 3 ) And Dead Sea Salt (sea salt) are mixed. In addition, the lactic acid bacteria fermentation mixture is obtained by fermenting the mixture with lactic acid bacteria.

The cosmetic composition of the present invention can be prepared through various methods, and according to an embodiment, a first step of preparing a mixture of green tea seed oil, centella extract, astaxanthin, lecithin, rice bran extract, ceramide 3, and dead sea salt , The mixture can be prepared by performing the second step of inoculating the lactic acid bacteria, stirring and fermenting the mixture, and the third step of preparing the lactic acid bacteria fermentation mixture by centrifuging the obtained fermented product and filtering the supernatant.

The mixture of the above manufacturing method includes 0.1 to 50 parts by weight of green tea seed oil, 0.1 to 25 parts by weight of centella asiatica extract, 0.1 to 30 parts by weight of astaxanthin, 0.1 to 15 parts by weight of lecithin, 0.1 to 30 parts by weight of rice bran extract, ceramide 3 0.1 to 25 parts by weight and 0.1 to 25 parts by weight of Dead Sea salt may be included.

At this time, if the green tea seed oil is less than 0.1 parts by weight, there may be a problem of lowering the efficacy such as antioxidant and skin barrier reinforcement, and even if it exceeds 50 parts by weight, there may be a problem that side effects, solubility in the formulation, and economy are lowered. If the extract is less than 0.1 parts by weight, there may be a problem that the efficacy of antioxidant, anti-inflammatory, whitening, etc. is lowered, and if it exceeds 25 parts by weight, only the manufacturing cost may increase, and if astaxanthin is less than 0.1 parts by weight, wrinkle improvement and whitening , If the effective effect such as antioxidant is weak, and if it exceeds 30 parts by weight, the particles are not uniform, or there may be a problem that the skin sensitivity increases and the stability decreases when the powder formulation is manufactured.If the lecithin is less than 0.1 part by weight, skin aging and There may be a problem that it is difficult to exhibit the effect of preventing skin deposition, and if it exceeds 15 parts by weight, there may be a problem that the slipperiness and emulsion stability are lowered, and if the rice bran extract is less than 0.1 part by weight, the effect of antioxidant and skin barrier strengthening, etc. There may be a problem of lowering, and if it exceeds 30 parts by weight, there may be a problem that the enhanced effect does not appear, and if ceramide 3 is less than 0.1 parts by weight, the effect such as moisturizing effect is weak, and if it exceeds 25 parts by weight, It may be difficult to dissolve and precipitate at low temperatures.If the Dead Sea salt is less than 0.1, there may be a problem that the effect on skin antioxidant and skin moisturization decreases, and if it exceeds 25 parts by weight, the Dead Sea salt is not sufficiently dissociated and There may be a problem of deteriorating stability.

Green tea seed oil is an oil extracted from green tea seeds and contains a large amount of vitamins A, B, and C. It reduces melanin pigmentation such as spots and freckles, so it clears the skin and has a whitening effect, sterilization, and sebum control. As it contains ingredients, it relieves skin problems, and tocopherol and amino acids prevent oxidation of cell membranes, so it has good effects on skin aging and moisturizing.

Centella asiatica extract is extracted from Centella asiatica and contains asiaticoside, madecassic acid and asiatic acid as main components, plays an important role in regulating the normalization of connective tissues in the skin and induces keratinocytes. It also promotes fast and healthy growth of tissue components. The molecular biological function is to promote the production and secretion of collagen I and III, and ultimately has the effect of restoring wounds by regulating collagen formation in damaged epithelium.

Astaxanthin (AST) is a type of pigment called carotenoide of the xanthophyll family, and is included in many red marine organisms such as algae, salmon, lobster, and shrimp. Astaxanthin acts as a precursor of vitamin A in the human body, and has antioxidant activity at least 10 times more than other carotenoids and 500 times more than α-tocopherol. In addition, by reducing the immune function activity due to cytokine induction activity, carcinogenesis by removing oxygen radicals and the process of spreading cancer, it has been proven that the prevention effect of ovarian cancer, liver cancer, etc.

Lecithin is one of the phospholipids containing glycerin phosphate, and is one of the main components that make up the biological membrane, and is contained in large amounts in egg yolk, soybean oil, liver, and brain. Lecithin is a substance that regulates moisture in cells, and when it is rich in lecithin, the skin becomes shiny and shiny. In addition, linoleic acid, linoleic acid, and the like, which are fatty acids that make up lecithin, contain unsaturated fatty acids, and are effective in lowering cholesterol levels.

Rice bran refers to a pulverized mixture of rind, seed skin, and aling layer, which is produced when brown rice is milled to make polished rice. The composition varies depending on the degree of degree, and if you pound a lot, fat, vitamin B1 phosphorus increases and fiber decreases. The standard chemical composition of rice bran is water 13.5%, protein 13.2%, fat 18.3%, sugar 38.3%, fiber 7.8%, ash 8.9%, and vitamin B1 contains 2.5mg of 100g, and contains a large amount of vitamin E. Has been. Rice bran extract (Oryza Sativa (Rice) Bran Extract) extracted from rice bran is an ingredient that provides moisture to the skin and provides moisture.

Ceramide is a component present in the stratum corneum of the skin epidermis and accounts for about 40 to 50% of the lipid component between keratinocytes. Ceramide inhibits evaporation of moisture from the skin by forming a multi-lamella structure between keratinocytes with lipid components between other keratinocytes and maintains moisture to soften the skin and keep the skin healthy. In addition, since the ceramide has a hydrophilic group and a lipophilic group at the same time, it can suppress evaporation of moisture in the skin, and acts as a skin barrier that blocks the penetration of foreign substances into the skin, thereby controlling inflammation and healing wounds.

Dead Sea salt (Sea salt/Mineral salt) is salt mined in the Dead Sea and belongs to the high mineral content in salt used as a cosmetic ingredient. General salt contains 97% of sodium chloride, but Dead Sea salt contains 40 to 50% of magnesium chloride, about 30% of sodium chloride and 12 to 15% of calcium chloride. Dead Sea salt functions as antibacterial and skin softening. Sodium chloride is physiologically indispensable to animals, and is included in body fluids to control the osmotic pressure of body fluids, and sodium, a constituent of sodium chloride, binds to phosphoric acid and can maintain the acid base balance of body fluids as a buffer material. It has a positive effect on the human body when applied to the skin as well as ingestion, and it has been reported to have superior skin moisturizing power than urea, a natural moisturizing factor component of the skin.Relieve various skin problems (soothe skin), remove skin waste And it is effective in removing dead skin cells, acne, wrinkles, and atopy.

In particular, since the composition of the mixture according to the present invention contains only EWG grades 1 to 2, it satisfies both the functionality as a cosmetic raw material and safety according to the grade, and it is not harmful according to the safety grade, so it is easy to apply to sensitive skin. It can also reduce concerns about toxic substances.

The EWG grade is a harmful grade for cosmetic ingredients published by the Environmental Working Group (EWG), a non-profit environmental civic group in the United States, and it is classified into grades 1 to 10 and classifies the harmfulness of the ingredients into grades 1 to 10 by investigating the harmfulness of cosmetic ingredients. The grades are classified into low-risk safety grades, grades 3 to 6 are classified as normal grades for attention, and grades 7 to 10 are classified as high-risk caution grades.

The lactic acid bacteria used for fermentation of the lactic acid bacteria may include Lactobacillus, Bacillus, and the like, but preferably include Lactobacillus, but are not limited thereto.

Strains refer to bacteria or fungi purely isolated and cultured, and a strain of the genus Lactobacillus may be used for the Lactobacillus, Lactobacillus sakei B2-16, Lactobacillus brevis, Lactobacillus plantarum (Lactobacillus plantarum P23), Lactococcus lactis (Lactococcus lactis A164), Bacillus coagulance (Bacillus coagulance) one or more may be selected from the group consisting of, but is not limited thereto. Bacillus strains can be used for the Bacillus genus, consisting of Bacillus licheniformis, Bacillus pumilus, Bacillus sonorensis, and Bacillus subtilis One or more may be selected from the group, but is not limited thereto.

The mixture may be a fermentation mixture prepared by fermenting with lactic acid bacteria. Fermentation refers to a process of decomposing organic matter using enzymes possessed by lactic acid bacteria, and it is known that raw materials that have undergone fermentation through lactic acid bacteria and fermentation strains increase various effects compared to before fermentation. Substances that have undergone fermentation can contain various amino acids, organic acids, and antioxidants that are good for the skin, and through the fermentation process, particles become smaller and toxicity is decomposed, thereby increasing absorption or alleviating side effects.

The cosmetic composition according to the present invention contains 0.001 to 90% by weight of the lactic acid bacteria fermentation mixture. It is preferable that the lactic acid bacteria fermentation mixture is contained within the range of 0.001 to 90% by weight relative to the total weight.If it is less than 0.001% by weight, there may be a problem that the effective effect of the lactic acid bacteria fermentation mixture may not be achieved, and when it exceeds 90% by weight, the effect There may be a problem in that only side effects such as a difficult problem of formulation, a decrease in the user's feeling of use, and an increase in skin irritation occur without increasing the amount of.

The lactic acid bacteria fermentation mixture according to the present invention is a skin ointment, cream, soft lotion, nutrient lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent , Lotion, balm, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, nutrition essence, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser It can be applied to any one formulation selected from, but is not limited thereto.

The cosmetic composition may contain a generally acceptable carrier, for example, oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc. may be appropriately blended, but are limited thereto. no.

The acceptable carrier may vary depending on the formulation. For example, when formulated as an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, or their Mixtures can be used.

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or mixtures thereof may be used as a carrier component. It may further comprise a propellant such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated as a solution or emulsion, a solvent, a solubilizing agent, or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil may be used, and in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.

When the cosmetic composition is formulated as a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the cosmetic composition is formulated with soap, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, a fatty alcohol, vegetable oil, glycerol, sugar, etc. Can be used.

The cosmetic composition is a fatty substance, an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, and a fragrance, which are commonly used in the industry depending on the quality or function of the final product. , Surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, commonly used in cosmetics It may additionally contain adjuvants commonly used in the field of cosmetology or dermatology, such as any other ingredients.

However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

In the cosmetic composition, in addition to the essential ingredients in each formulation, other ingredients may be appropriately blended within a range that does not impair the object according to the present invention depending on the type or purpose of use, desired skin improvement effect, and the like.

Hereinafter, the present invention will be further described through examples and experimental examples. These examples are for explaining the present invention more specifically, and the scope of the present invention is not limited to these examples.

1. Manufacturing example

After homogeneously mixing the extracts and oils and commercially available components obtained according to the conventional natural product extraction method and oil extraction method in the composition ratio shown in Tables 1 and 2 below, Comparative Examples 1 to 7, Experimental Examples 1 to Experimental Examples Used in 8.

In Experimental Examples 9 to 16, the mixtures of Experimental Examples 1 to 8 were autoclaved at 120° C. for 60 minutes, and then lactic acid bacteria Lactobacillus strain was inoculated, stirred at 27° C. at 150 rpm, and fermented for 72 hours. The obtained fermentation product was centrifuged to remove insoluble substances, and the supernatant was collected and filtered through a 0.45 μm filter to prepare a lactic acid fermentation mixture of Experimental Examples 9 to 16.

ingredient Control Example 1 Control Example 2 Control Example 3 Control Example 4 Control Example 5 Control Example 6 Control Example 7 mix
water Green Tea Seed Oil 0.0 38.9 41.2 36.8 41.2 38.9 38.9 Centella asiatica extract 15.4 0.0 11.8 10.5 11.8 11.1 11.1 Astaxanthin 23.1 16.7 0.0 15.8 17.6 16.7 16.7 lecithin 7.7 5.6 5.9 0.0 5.9 5.6 5.6 Rice bran extract 23.1 16.7 17.6 15.8 0.0 16.7 16.7 Ceramide 3 15.4 11.1 11.8 10.5 11.8 0.0 11.1 Dead Sea Salt 15.4 11.1 11.8 10.5 11.8 11.1 0.0

ingredient Experimental Example 1 Experimental Example 2 Experimental Example 3 Experimental Example 4 Experimental Example 5 Experimental Example 6 Experimental Example 7 Experimental Example 8 mix
water Green Tea Seed Oil 35.0 60.0 25.3 24.7 29.5 24.7 25.3 25.3 Centella asiatica extract 10.0 6.2 35.0 7.1 8.4 7.1 7.2 7.2 Astaxanthin 15.0 9.2 10.8 40.0 12.6 10.6 10.8 10.8 lecithin 5.0 3.1 3.6 3.5 20.0 3.5 3.6 3.6 Rice bran extract 15.0 9.2 10.8 10.6 12.6 40.0 10.8 10.8 Ceramide 3 10.0 6.2 7.2 7.1 8.4 7.1 35.0 7.2 Dead Sea Salt 10.0 6.2 7.2 7.1 8.4 7.1 7.2 35.0 Lactobacillus fermentation mixture Experimental Example 9 Experimental Example 10 Experimental Example 11 Experimental Example 12 Experimental Example 13 Experimental Example 14 Experimental Example 15 Experimental Example 16

2-1. Formulation Example 1

Cosmetics were prepared according to a conventional cosmetic manufacturing method in the composition ratios of Tables 3 and 4 below.

ingredient Toner formulation Lotion formulation Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Purified water 89 89 89 88.39 84.46 86.644 81.224 75.204 Butylene Glycol 5 5 5 5 5 3 3 3 glycerin 2 2 2 2 2 2 2 2 Pentylene glycol - - - - - - - - Carbomer 0.05 0.05 0.05 0.05 0.05 0.1 0.1 0.1 1,2-hexanediol 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 c12-14 pares-12 One One One One One 0.5 0.5 0.5 Control Example 1 0.5 - - - - - - - Experimental Example 1 - 0.5 - - - - - - Experimental Example 2 - - 0.5 - - 0.1 - - Experimental Example 9 - - - One - - 5 - Experimental Example 10 - - - - 3 - - 10 Beeswax - - - - - 0.5 0.5 0.5 Shea Butter - - - - - 2 2 2 Cetearyl alcohol - - - - - One One One C14-22 alcohol - - - - - 1.193 1.193 1.193 C12-20 alkyl glucoside - - - - - 0.003 0.003 0.003 Glucose - - - - - 0.01 0.01 0.01 Glycerylcaprylate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopherol 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Arginine 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Spices 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Niacinamide - - - 0.1 2 - One 2 Adenosine - - - 0.01 0.04 - 0.02 0.04 Alpha Bisabolol - - - - - 0.5 - - Retinyl Palmitate - - - - - - - -

ingredient Cream formulation Chestnut Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Purified water 78.009 64.999 46.469 0 0 0 Butylene Glycol 5 5 5 - - - glycerin 2 2 2 - - - Pentylene glycol One One One - - - Carbomer 0.3 0.3 0.3 - - - 1,2-hexanediol 1.2 1.2 1.2 - - - c12-14 pares-12 0.5 0.5 0.5 - - - Control Example 1 - - - - - - Experimental Example 1 - - - - - - Experimental Example 2 One - - 40 - - Experimental Example 9 - 15 - - 60 - Experimental Example 10 - - 30 - - 80 Beeswax One One One 28.8 18.3 9 Shea Butter 3 3 3 30 20 8.8 Cetearyl alcohol 1.5 1.5 1.5 - - - C14-22 alcohol 2.385 2.385 2.385 - - - C12-20 alkyl glucoside 0.6 0.6 0.6 - - - Glucose 0.006 0.006 0.006 - - - Glycerylcaprylate 0.5 0.5 0.5 - - - Tocopherol 0.5 0.5 0.5 0.5 0.5 0.5 Arginine 0.3 0.3 0.3 - - - Spices 0.2 0.2 0.2 0.2 0.2 0.2 Niacinamide - - 2 - - - Adenosine - 0.01 0.04 - - - Alpha Bisabolol - - 0.5 0.5 - 0.5 Retinyl Palmitate One - One - One One

2-2. Formulation Example 2

The lactic acid bacteria fermentation mixture of Experimental Example 9 250 mg, dextrin 195 mg, and glucose 200 mg were mixed, granulated using a fluid bed dryer, and then 5 mg of sugar ester was added thereto, followed by tableting with a tablet press to prepare tablets. This tablet was called Example 13.

2-3. Formulation Example 3

After mixing 100 mg of the lactic acid bacteria fermentation mixture of Experimental Example 9, 10 g of glucose and 2 g of citric acid, 185 g of purified water was added, and the bottle was filled to 200 ml. After sterilization at 90 ℃ 4 hours to prepare a beverage. This beverage was referred to as Example 14.

3. Test example

1) Evaluation of whitening efficacy (tyrosinase activity inhibition test)

In order to test the whitening effect of the control examples and experimental examples prepared in Tables 1 and 2, the degree of inhibition of the function of an enzyme called tyrosinase was evaluated. Tyrosinase is an enzyme that helps the production of melanin by promoting the oxidation process of a substance called tyrosine in the body. Melanin is a black polymer. When melanin is formed by the above oxidation in a living body and moves to the upper layer of the skin, the skin turns black and creates spots and freckles. It was evaluated by applying a method of testing the whitening effect (Pomerantz S.H, J Biochem, 24:161-168, 1966) by measuring the degree of inhibition of the action of tyrosinase. The test method is as follows.

0.9 ml of each of the samples in Tables 1 and 2, 1.0 ml of 0.1 M phosphate buffer (pH 6.8), and 1.0 ml of 1.5 mM L-tyrosine solution were added, and then maintained at 37° C. for 10 minutes. After adding 0.1 ml of mushroom tyrosinase (1,500 units/ml) and reacting for 10 minutes at 37°C, the absorbance was measured at 475 nm using a microplate reader (iMark ^TM , Biorad, USA) to prevent tyrosinase. The inhibition rate was measured.

The control for the tyrosinase activity inhibition test was measured in the same way by putting a buffer solution instead of a sample solution, and a buffer solution was added instead of tyrosinase to obtain each color correction value for the sample and the control group. .

In the comparative group used in the experiment to determine the effect, Kojic acid (synthetic, Sigma), which is known to have excellent whitening effect from various research results, was compared, and the tyrosinase inhibition rate was calculated numerically using the following Equation 1. It is shown in Table 5.

In Table 5, IC ₅₀ is the concentration of the sample required to inhibit the tyrosinase activity by 50%, and the smaller the value, the higher the inhibition rate.

Item Tyrosinase inhibition rate (IC _50, ug/ml) Control Example 1 35.6 Control Example 2 38.4 Control Example 3 39.5 Control Example 4 34.1 Control Example 5 41.1 Control Example 6 33.4 Control Example 7 35.7 Experimental Example 1 24.9 Experimental Example 2 30.2 Experimental Example 3 33.6 Experimental Example 4 34.3 Experimental Example 5 39.2 Experimental Example 6 35.7 Experimental Example 7 28.3 Experimental Example 8 30.2 Experimental Example 9 23.0 Experimental Example 10 25.0 Experimental Example 11 28.9 Experimental Example 12 29.2 Experimental Example 13 34.2 Experimental Example 14 30.9 Experimental Example 15 23.7 Experimental Example 16 25.2 Kojic acid 48.2

According to the results of Table 5, Experimental Examples 1 to 8 had higher whitening effects than Control Examples 1 to 7, and the tyrosinase inhibition rate in Experimental Examples 9 to 16 fermented with lactic acid bacteria was higher. . It can be expected that the mixture and the composition containing the lactic acid bacteria fermentation mixture obtained by fermenting the mixture with lactic acid bacteria enhance the whitening effect.

2) Antioxidant effect measurement

Free radical scavenging test and active oxygen scavenging test were conducted to confirm the antioxidant effect of the control examples and experimental examples prepared in Tables 1 and 2, and to determine the effect, Ascorbic acid, which is known to have excellent antioxidant effect, was used. It was used as a control group.

2-1) Free radical elimination test

The free radical scavenging test uses that the absorbance of stable DPPH shows the maximum absorbance at 517 nm, and the free radical DPPH is erased by the sample and becomes pale yellow from purple (the free radical scavenging rate increases) at the wavelength of 517 nm. By applying the decrease in absorbance, it was carried out by the following calibration test method.

First, 1 ml of a 0.1 mM 2,2-diphenyl-1-picryl-hydrazyl radical (DPPH, Sigma) solution was diluted with methanol to an appropriate concentration, 1 ml was mixed, and left at room temperature for 15 minutes. Absorbance was measured at a wavelength of 517 nm using a microplate reader (iMark ^TM , Biorad, USA).

In the free radical scavenging test, the control was measured in the same manner by adding 1 ml of DPPH and 1 ml of methanol, and 1 ml of methanol and 1 ml of the sample were added to obtain color correction values for the sample and the control.

The free radical elimination rate was calculated using Equation 3 below and shown in Table 6 below. In Table 6, FSC ₅₀ is the concentration of the sample required to remove 50% of free radicals, and the smaller the value, the higher the antioxidant activity.

3-2) Free radical scavenging test

The active oxygen scavenging test uses the generation of active oxygen by the enzymatic reaction of xanthine/xanthine oxidase (Sigma) to determine the change in absorbance by oxidation of nitroblue tetrazolium (NBT) by active oxygen. By measuring, it is possible to know the scavenging ability of active oxygen.

Na ₂ CO ₃ 2.4 ml, xanthine (Sigma) 0.1 ml, ethylenediamine tetraacetic acid (EDTA) 0.1 ml, bovine serum albumin (BSA, Sigma) 0.1 ml, NBT 0.1 ml, and sample 0.1 ml are added to the vortex mixer (MS1 Minishaker, IKA). , Germany) after voltexing and allowed to stand at 25°C for 10 minutes, add 0.1 ml of xanthine oxidase and react for 30 minutes at 37°C, and then add 6 mM CuCl ₂ to stop the reaction, microplate reader (iMark ^TM ,, Biorad, USA) was used to measure absorbance at a wavelength of 550 nm.

The control group in the reactive oxygen scavenging activity test was measured in the same way by adding tertiary distilled water instead of the sample solution, and tertiary distilled water was added instead of the xanthine oxidase solution to obtain color correction values for the extracted sample and the control.

The active oxygen scavenging rate was calculated numerically using Equation 3 and shown in Table 6 below. In Table 6, SC ₅₀ is a sample concentration required to remove 50% of active oxygen, and the smaller the value, the stronger the antioxidant activity.

Item Free radical elimination rate
FSC50 (ug/ml) Active oxygen scavenging rate
SC50 (ug/ml) Control Example 1 23.1 30.1 Control Example 2 12.4 18.4 Control Example 3 28.1 35.9 Control Example 4 11.9 19.8 Control Example 5 12.0 17.0 Control Example 6 14.0 16.9 Control Example 7 22.2 40.4 Experimental Example 1 8.6 14.0 Experimental Example 2 13.4 24.0 Experimental Example 3 9.5 16.4 Experimental Example 4 9.7 33.0 Experimental Example 5 9.3 17.0 Experimental Example 6 10.4 16.5 Experimental Example 7 11.6 14.4 Experimental Example 8 18.4 33.7 Experimental Example 9 5.2 7.0 Experimental Example 10 11.3 20.1 Experimental Example 11 8.4 14.6 Experimental Example 12 8.9 30.1 Experimental Example 13 7.5 16.0 Experimental Example 14 8.9 14.3 Experimental Example 15 10.5 12.9 Experimental Example 16 16.4 30.7 Ascorbic acid 6.5 10.3

According to the results of Table 6, the free radical scavenging rate or the free radical scavenging rate of Experimental Examples 1 to 8 was significantly higher than that of Comparative Examples 1 to 7, and in Experimental Examples 9 to 16 fermented with lactic acid bacteria. The resulting value was even higher. It can be expected that the mixture and the composition containing the lactic acid fermentation mixture obtained by fermenting the mixture with lactic acid bacteria enhance the antioxidant efficacy.

3) Anti-inflammatory evaluation

3-1) Nitrogen monoxide (NO) production inhibitory activity measurement

In order to confirm the anti-inflammatory effect of the composition according to the present invention, the ability to inhibit the production of nitrogen monoxide (NO) causing inflammation was analyzed.

RAW 264.7 cells were placed in a 48-well plate and allowed to adhere for 20 hours. Lipopolysaccharide (LPS, 1 μg/ml) was treated to induce NO production, and then the ability to inhibit NO production was analyzed through a Greiss reaction. Grease reaction is a method of quantitatively analyzing the amount of NO generated from cultured cells and released into the medium. After 24 hours of sample treatment, 100 μL of cell culture medium was dispensed into a 96-well plate, and then grease reaction solution A (5% phosphoric acid). 1% sulfanilamide solution dissolved in the solution) and B (0.1% naphthylenediaminedihydrochloride) buffer solution were mixed at a ratio of 1:1 and dispensed into a 96-well plate by 100 μL, and the color change due to NO generation was observed on a multi-plate The absorbance was measured at 540 nm using a reader, and the value was expressed as the average value of 5 repeated experiments. In order to prepare the basis for the judgment on the effect, 2-amino-4-methypyridine, which is known to inhibit NO production in several studies, was used as a comparative group, and the results are shown in Table 7.

3-2) Measurement of inhibitory activity to produce pro-inflammatory cytokines (tumor necrosis factor-alpha; TNF-α, interleukin-6; IL-6)

In addition, in order to confirm the anti-inflammatory effect of the composition according to the present invention, it was to analyze the inhibitory activity of pro-inflammatory ytokines (TNF, IL-6) production.

RAW 264.7 cells were placed in a 48-well plate and allowed to adhere for 20 hours. Each extract and Lipopolysaccharide (LPS, 1 μg/mL) were treated together and incubated for a certain time. Thereafter, the culture medium was centrifuged (12,500 rpm, 3 min) to measure the content of pro-inflammatory cytokine produced in the obtained supernatant, and Dexamethason was used as a control group to determine the effect thereof. All samples were stored frozen (-20°C) until quantification. Proinflammatory cytokines were quantified using a mouse enzymelinked immnunosorbent assay (ELISA) kit (R&D Systems Inc, Minneapolis, MN, USA), and the r2 value of the standard curve for the standard was 0.99 or higher, and the results are shown in Table 7.

Item NO production inhibition TNF-alpha IL-6 Control Example 1 40.6 38.0 45.4 Control Example 2 54.6 39.0 56.8 Control Example 3 46.1 44.0 49.6 Control Example 4 44.0 37.3 46.3 Control Example 5 27.9 25.0 33.8 Control Example 6 50.6 48.6 54.6 Control Example 7 46.6 44.4 48.6 Experimental Example 1 61.0 55.1 62.0 Experimental Example 2 49.6 50.0 53.6 Experimental Example 3 58.6 47.6 60.6 Experimental Example 4 53.4 40.4 60.4 Experimental Example 5 52.0 47.7 54.0 Experimental Example 6 46.9 46.0 53.9 Experimental Example 7 59.6 50.0 65.6 Experimental Example 8 54.7 46.6 68.7 Experimental Example 9 68.4 59.9 73.4 Experimental Example 10 57.8 50.0 60.8 Experimental Example 11 62.6 48.0 66.6 Experimental Example 12 59.4 51.3 63.4 Experimental Example 13 60.0 51.7 64.0 Experimental Example 14 59.9 50.1 66.7 Experimental Example 15 60.6 49.0 63.8 Experimental Example 16 60.9 48.7 66.0 2-amino-4-methypyridine (5 ppm) 65.0 - 65.0 Dexamethason (100 ppm) - 51.3 71.6

According to the results of Table 7, the anti-inflammatory efficacy of Experimental Examples 1 to 8 was superior to that of Control Examples 1 to 7, and Experimental Examples 9 to 16 fermented with lactic acid bacteria were more excellent. It can be expected that the mixture and the composition containing the lactic acid fermentation mixture obtained by fermenting the mixture with lactic acid bacteria enhances anti-inflammatory efficacy.

4) Usability evaluation

For the cosmetic compositions prepared according to Comparative Example 1, Comparative Example 2, Example 2, Example 3, Example 8 and Example 11, the feeling of use of the subject was evaluated for the effects of skin barrier, moisturizing, elasticity, and skin absorption. . For ingestable tablets and beverages prepared according to Formulation Example 2 and Formulation Example 3, Examples 13 and 14, the feeling of use of the subject was evaluated for the effects of skin barrier, moisturizing, elasticity, and skin absorption. Comparative Example 1, Comparative Example 2, Example 2, Example 3, Example 8 and Example 11 were used for 12 weeks, and Examples 13 and Example 11 were used for 12 weeks, each of 5 for a total of 40 healthy women aged 25 to 40 years. 14 is an objective evaluation of the degree of improvement of skin barrier, moisture, elasticity, and skin absorption effect felt by the subject by ingesting it for 12 weeks within 30 minutes after meals three times a day. = Normal, 2 = bad, 1 = very bad) and evaluated, and the results are shown in Table 8.

Evaluation item No. Comparative Example 1 Comparative Example 2 Example 2 Example 3 Example 8 Example 11 Example 13 Example 14 Skin barrier
Improvement effect
(A) One One 3 4 3 5 4 4 3 2 2 3 5 3 5 4 4 3 3 One 2 4 3 4 5 3 4 4 2 2 4 3 5 5 4 4 5 One 2 5 3 4 5 3 3 Average 1.4 2.4 4.4 3 4.6 4.6 3.6 3.4 Moisturizing effect
(B) One 2 3 4 3 5 4 3 4 2 2 2 4 2 5 5 4 3 3 One 2 5 3 5 5 3 3 4 3 3 5 3 4 4 4 3 5 2 2 4 5 5 5 3 3 Average 2 2.4 4.4 3.2 4.8 4.6 3.4 3.2 Elasticity effect
(C) One One One 2 3 4 5 3 4 2 One 3 2 4 4 5 4 4 3 2 2 3 3 4 4 3 3 4 One 3 2 5 5 3 5 3 5 2 One One 3 5 5 4 3 Average 1.4 2 2 3.6 4.4 4.4 3.8 3.4 Skin absorption effect
(D) One One 2 5 3 4 3 3 4 2 One 2 4 4 4 5 3 4 3 2 2 5 3 5 4 4 3 4 One 2 5 3 5 4 3 3 5 One 3 5 4 4 4 3 4 Average 1.2 2.2 4.8 3.4 4.4 4 3.2 3.6

Looking at the results of Table 8, through Comparative Example 1 and Comparative Example 2, compared with no specific ingredients in the mixture, green tea seed oil, centella extract, astaxanthin, lecithin, rice bran extract, ceramide 3, and dead sea salt were all included. The user's sense of satisfaction with the mixture was high, and the user's satisfaction was significantly different depending on whether or not fermentation with lactic acid bacteria was fermented through Comparative Example 2 and Example 2. In addition, the feeling of use was somewhat low when specific ingredients in the mixture were excessively contained through Examples 2 and 3, and user satisfaction was high even when various formulations were used through Examples 8 and 11. Able to know.

In addition, in the case of ingesting the lactic acid bacteria fermentation mixture of Examples 13 and 13, the user's satisfaction through the answer that the skin barrier improvement, moisturizing, elasticity, and skin absorption effects were improved when compared with Comparative Example 1 and Comparative Example 2. It can be seen that this was high.

Summarizing the above results, the lactic acid bacteria fermentation mixture in which a mixture containing green tea seed oil, centella asiatica extract, astaxanthin, lecithin, rice bran extract, ceramide 3, and dead sea salt is fermented with lactic acid bacteria is a variety of examples containing individual components. By comparison, the effect of improving skin whitening, skin moisturizing, antioxidant, anti-inflammatory, skin barrier strengthening and skin absorption function was remarkably excellent, and even when used in various formulations such as toners, lotions, creams, balms, etc. It was very useful to apply to cosmetics, and it was confirmed that the aforementioned improvement effect was excellent even when applied to ingestible tablets and beverages.

The above description is merely illustrative of the present invention, and those of ordinary skill in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form within the scope not departing from the essential characteristics of the present invention. I will be able to. Therefore, the disclosed embodiments and experimental examples should be considered from a descriptive point of view rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (5)

Green tea seed oil (Camellia Sinesis seed oil), Centella asiatica extract, Astaxanthin, Lecithin, Oryza Sativa (rice) bran extract, Ceramide 3 and Dead Sea Contains a lactic acid bacteria fermentation mixture obtained by fermenting a mixture containing sea salt with lactic acid bacteria,
The lactic acid bacteria is a cosmetic composition, characterized in that it contains Lactobacillus (Lactobacillus).
delete The method of claim 1,
The mixture,
Green tea seed oil (Camellia Sinesis seed oil) is 0.1 to 50 parts by weight, Centella asiatica extract is 0.1 to 25 parts by weight, Astaxanthin is 0.1 to 30 parts by weight, Lecithin is 0.1 to 15 parts by weight, rice bran extract (Oryza Sativa (rice) bran extract) is 0.1 to 30 parts by weight, ceramide 3 is 0.1 to 25 parts by weight, and the sea salt is a mixture of 0.1 to 25 parts by weight. Cosmetic composition characterized in that.
The method of claim 1,
The cosmetic composition is a cosmetic composition comprising the amount of 0.001 to 90% by weight of the lactic acid bacteria fermentation mixture based on the total weight.
The method of claim 1, wherein the cosmetic composition is a skin ointment, cream, softening lotion, nutrient lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner , Astringent, lotion, balm, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, nutrition essence, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser Cosmetic composition, characterized in that any one formulation selected from the group consisting of.